MC09g1270 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1270
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPyruvate kinase family protein
LocationMC09: 18811954 .. 18816148 (+)
RNA-Seq ExpressionMC09g1270
SyntenyMC09g1270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCTTTTTTCAATTTTTAGGTCAATATTTCGACAAATATTCTCTGTTGAATATATAGTTTTACGGAGCGGTATAGGGAAGAACCAGAAACGGTGGTAGCGGAATCCTGAGGCCTTCTCCATTTCACACAACTCCACGCTACAGACACAGCTGAAGAACAAGAACAAGAACAATAACAACAAGAACAAGTTCAGTTGATCTCAAATCTGTTCTTCACCAACACGAGGTAGATGAAGATCTCTCTCGGATATTCATCTTTGTCTCCGATTTTTTCTGTTTCTGTTTTTCTTTTTCTCAAACTTGTCATAGATTTTGATGTTTTGATGTTTCCTTCCTAGTTTTCTGTTCCTTGAGTTATCAGTTAATCTCTACTCTTCGAGTTTTTCGGATCTCATTCTATTTACCTATCCTGGATTGATTGATTTTTTTTTCCCATTTTGTTTTTCTTTTTGTATATATGTTTCACGTTTTTGGGTTGATTTACAGAACCCTAGCGAAGAGGAAGCAGTAGCAAATGGCCAATATTGATATTGAGGGGATTCTCAAGGAACTTCCTAGTGATGGACGTATCCCTAAAACGAAAATTGTCTGTACTCTGGGACCTGCTTCCCGATCGGTGCCTATGATTGAGAAGCTTCTCAGGGCTGGCATGAATGTGGCTCGTTTCAATTTCTCTCATGGTACCCATGAGTATCATCAAGAAACCTTGAACAATCTCAGGGCCGCCATGCAGAATACTCAAATCCTTTGCGCCGTCATGCTCGATACCAAGGTATTTTTCTTTTCCCGCTATGGTCTCTGTTAAAAAATGACGAGGCATGCGATAATTCGAGATGGTATTTTATGTTTCTCTAATATTTATCATTGGTTCTGGCTTGGGTGATTTGAAAATGGATGCAAGAACGATCTGGATTATAGATTGTGGAATATGAAAATTCCTGATGTTTTGCTTGTCAATGCACTTGCAAGGTTAAGAACCTCGTTGAAATTGTTTTCTCATTTGTTTCACGTCAAGATCCAATGACAATTGACACCATCTTAGTTGTACGAAAACCACCATATCAAATACAATGGTGACACTTGGGGCAAATCTTGTATTATTCGCCTTTCGGTGTCTCTGGAGATTTTTTTCCCTATTGCAAATAAGTAAATTTTGGGTTCCCTTTGAAATTCAGACAACCTGATAAGCTGATATGCAGTTTACGAAAAATATTCAATCTATATCTTAGTCTGAGAATTAGTTGCGCTGGCTGCATGATTTTTCACTTTAAATCTGGTACATATTGACGGATTTTCAAAAGCTATATATGGCCTCTAAATAAGTGATTGTGGAGTAACAATTTGCTGTTAGTTTGTGTAGTTGGATGGTATTGATATGTATGAATATCATCATATTTGGCAGTTTTGTTGCTGGTGGTAGATATACTGGCACAGCTCCTTTTGGTATTTTCACTTGCCTGTGGTCAGGTTAATATAGTGTTTGGAAGCCTTTTTATTTCTTTTTCCAACTCCTGAACTTTAAATTCTGAAGTCTTACCTATCTTGCAACACTTTCATGATCCTTTGCGTGTAAAATGTCTAATGCAAAGACGATGTCTTGTAGGAGAGTTTGCCTAATACTGTCTTTTTGCTACTATTCATGCAAACATTTTAACTAGAGAACTTAAACATCTGTATAGTCCTTTGGTTATCTGAGGCGACACATGTTTGTTTCCTTGGTAAAGTTGGAGCTTGAGGATCAAATATATACTGTATTAAAAAATACTTGCACTTCTGAAAAAAAAATGCATCAAGTTAAAAAAATTCCTGGCGTACTAGGTTCATTTAATTTTTTGTTTTTTTTTGGCATTTGCATACTGAATTCTCATGATGCTTCTCCTCTTATTCACCATGGTAGGGACCAGAGATTCGAACTGGTTTCCTGAAGGATGGGAAACCTATTCAACTGAAGGAAGGTCAGGAAGTTATCATAACTACAGATTATAGCATCAAGGGGGATGAGAAAATGATCTCAATGAGTTATCAGAAGCTTGCTGTTGACTTGAAGCCAGGAAATACTATATTGTGCTCAGATGGCACCATCACCCTCACTGTCCTGTCTTGTGATGCAGTAGGTGGGACGGTGAGATGTCGTTGTGAAAACACTGCCATGTTGGGAGAAAGAAAGAATGTGAATCTTCCTGGTGTAGTGGTGGATCTTCCTACTCTGACAGATAAGGATAAAGAGGATATTCTCGATTGGGGTGTTCCCAACAGTATCGATATGATTGCTCTTTCATTTGTCCGTAAGGGTTCTGATCTAGTCAATGTCCGCAAGGTTCTTGGCCCCCATTCAAAGCATATAAAACTGATGTCAAAGGTCTGTATTGTGCTTCCAGTTCCAGTTCTTATTTTATTTATCTACAATGATAATTATCATCATCTGTTGCTTCTTATGGTTAGACAGTCTTGGATAGTTTTAGACATTGCATGCGGTTGCTTGACTACTTTGTTATTTAGGTTAGGAATTGTACATTTTCATCGTGGAAATCAGTATTACACGACTAAATGCATTAGGAGCTGCTCAGCTGGCTTTGATTTGGTGCTTTCTGCCTTGCGAAATCCTTATTTTGTTGTTGCTTCCATGTATGCATGCTTTGTGCACAAAAGTTTTGGATTCTTCGTTTGATTCATACATCAATGTGGGCTAGTTGAGTGGGTTGTCAGTTAATGAGTTGTTACATTTGATGCCAAGGTATTGCTCGCAACCATATTGGGCATTTTATATGCATACTTTCAATATTTAGTCTTTCTTTGTACATGCTTTTCATCTAGTATATTTCTTACTTTAAAAACAATGTTTATTTCCAATATTGTTGCATTACATATTGTTTGGATCTAATGGTTATTTATCTTTTGCTGTGGATACCCAAGGTTGAGAACCAGGAGGGAGTTATCAATTTTGATGAAATCTTGCGCGAGACTGATGCATTCATGGTTGCTCGAGGTGATCTTGGAATGGAAATTCCAGTTGAGAAGATCTTCTTGGCGCAGAAAATGATGATTTACAAGTGTAATCTTGCTGGCAAACCTGTGGTTACAGCCACCCAAATGCTTGAATCAATGATCAAATCTCCACGGCCAACTCGTGCAGAAGCAACAGATGTAGCTAACGCCGTTCTTGATGGCACTGATTGTGTTATGCTTAGTGGTGAGAGTGCAGCTGGGTCATATCCTGAACTTGCTGTCAAGATCATGGCTCGTATTTGTATTGAAGCAGAATCATCCTTGGACTATGGGGTCATCTTTAAGGAGATGATAAGGGCCACCCCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCAGCTGTTCGAACCGCTAACAAGGCAAAAGCGAAGCTCATTGTCGTGTTGACTCGTGGTGGAACCACAGCAAAGTTGGTTGCCAAGTATAGGCCAGCTGTTCCGATCCTCTCAGTCGTCGTCCCTGTTTTAACTACTGATTCTTTTGATTGGACCTGCAGCGATGAGGCCCCAGCCCGACACAGCCTGATCCACAGGGGCTTGATTCCCATACTTGCTGAAGGATCTGCAAAGGCCACTGATGCCGAATCCACAGAAGTGATACTAGAAGCTGCTCTGAAATCAGCCATGGGAAAGGGACTGTGCAATCCTGGCGACGCCATTGTTGCACTTCACCGTATCGGAGCTGCCTCCGTCATCAAGATATGCATCGTGAAGTGAATAAAATAGACAAGCGAAATTCGGAATCGCTCCCAGGTGTGCGTTCCTTATCTCTATTTACCATTGCAGTCGGATCTCAATCTATCCTTTTTTGCTGATTATTACAAGTATAGTGATTTAGTATTTCATCTAAAGAGGGCTGAACCGGTATTTTGATTTTCTTGTTGTGAGAAGGGGGTGGTGGATATTCCCCTGGATTTTTTTGGACTGATTGCTGTGTAATTTTGAATAGTGAAGAGTTTATGCTATTAATCCAATCTTCTTCTTTTAGGGAGATGAAGAATTGGATCAGACTTTCATCAATTCCCCAAAATTTTCTGCTCTACTTTCAGTTTGTAGCATTCTAAATCATTAGTATGGGAAAATTTTGGTCGTAATAGATCGACTTTCATGCTAGTTCTTGCTCCAGCATGAAAGTTCCATTTCAAGGAAGGACGAATAGCTACTTCCTCCATTCACAATTTGACA

mRNA sequence

ATTTCTTTTTTCAATTTTTAGGTCAATATTTCGACAAATATTCTCTGTTGAATATATAGTTTTACGGAGCGGTATAGGGAAGAACCAGAAACGGTGGTAGCGGAATCCTGAGGCCTTCTCCATTTCACACAACTCCACGCTACAGACACAGCTGAAGAACAAGAACAAGAACAATAACAACAAGAACAAGTTCAGTTGATCTCAAATCTGTTCTTCACCAACACGAGAACCCTAGCGAAGAGGAAGCAGTAGCAAATGGCCAATATTGATATTGAGGGGATTCTCAAGGAACTTCCTAGTGATGGACGTATCCCTAAAACGAAAATTGTCTGTACTCTGGGACCTGCTTCCCGATCGGTGCCTATGATTGAGAAGCTTCTCAGGGCTGGCATGAATGTGGCTCGTTTCAATTTCTCTCATGGTACCCATGAGTATCATCAAGAAACCTTGAACAATCTCAGGGCCGCCATGCAGAATACTCAAATCCTTTGCGCCGTCATGCTCGATACCAAGGGACCAGAGATTCGAACTGGTTTCCTGAAGGATGGGAAACCTATTCAACTGAAGGAAGGTCAGGAAGTTATCATAACTACAGATTATAGCATCAAGGGGGATGAGAAAATGATCTCAATGAGTTATCAGAAGCTTGCTGTTGACTTGAAGCCAGGAAATACTATATTGTGCTCAGATGGCACCATCACCCTCACTGTCCTGTCTTGTGATGCAGTAGGTGGGACGGTGAGATGTCGTTGTGAAAACACTGCCATGTTGGGAGAAAGAAAGAATGTGAATCTTCCTGGTGTAGTGGTGGATCTTCCTACTCTGACAGATAAGGATAAAGAGGATATTCTCGATTGGGGTGTTCCCAACAGTATCGATATGATTGCTCTTTCATTTGTCCGTAAGGGTTCTGATCTAGTCAATGTCCGCAAGGTTCTTGGCCCCCATTCAAAGCATATAAAACTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAATTTTGATGAAATCTTGCGCGAGACTGATGCATTCATGGTTGCTCGAGGTGATCTTGGAATGGAAATTCCAGTTGAGAAGATCTTCTTGGCGCAGAAAATGATGATTTACAAGTGTAATCTTGCTGGCAAACCTGTGGTTACAGCCACCCAAATGCTTGAATCAATGATCAAATCTCCACGGCCAACTCGTGCAGAAGCAACAGATGTAGCTAACGCCGTTCTTGATGGCACTGATTGTGTTATGCTTAGTGGTGAGAGTGCAGCTGGGTCATATCCTGAACTTGCTGTCAAGATCATGGCTCGTATTTGTATTGAAGCAGAATCATCCTTGGACTATGGGGTCATCTTTAAGGAGATGATAAGGGCCACCCCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCAGCTGTTCGAACCGCTAACAAGGCAAAAGCGAAGCTCATTGTCGTGTTGACTCGTGGTGGAACCACAGCAAAGTTGGTTGCCAAGTATAGGCCAGCTGTTCCGATCCTCTCAGTCGTCGTCCCTGTTTTAACTACTGATTCTTTTGATTGGACCTGCAGCGATGAGGCCCCAGCCCGACACAGCCTGATCCACAGGGGCTTGATTCCCATACTTGCTGAAGGATCTGCAAAGGCCACTGATGCCGAATCCACAGAAGTGATACTAGAAGCTGCTCTGAAATCAGCCATGGGAAAGGGACTGTGCAATCCTGGCGACGCCATTGTTGCACTTCACCGTATCGGAGCTGCCTCCGTCATCAAGATATGCATCGTGAAGTGAATAAAATAGACAAGCGAAATTCGGAATCGCTCCCAGGTGTGCGTTCCTTATCTCTATTTACCATTGCAGTCGGATCTCAATCTATCCTTTTTTGCTGATTATTACAAGTATAGTGATTTAGTATTTCATCTAAAGAGGGCTGAACCGGTATTTTGATTTTCTTGTTGTGAGAAGGGGGTGGTGGATATTCCCCTGGATTTTTTTGGACTGATTGCTGTGTAATTTTGAATAGTGAAGAGTTTATGCTATTAATCCAATCTTCTTCTTTTAGGGAGATGAAGAATTGGATCAGACTTTCATCAATTCCCCAAAATTTTCTGCTCTACTTTCAGTTTGTAGCATTCTAAATCATTAGTATGGGAAAATTTTGGTCGTAATAGATCGACTTTCATGCTAGTTCTTGCTCCAGCATGAAAGTTCCATTTCAAGGAAGGACGAATAGCTACTTCCTCCATTCACAATTTGACA

Coding sequence (CDS)

ATGGCCAATATTGATATTGAGGGGATTCTCAAGGAACTTCCTAGTGATGGACGTATCCCTAAAACGAAAATTGTCTGTACTCTGGGACCTGCTTCCCGATCGGTGCCTATGATTGAGAAGCTTCTCAGGGCTGGCATGAATGTGGCTCGTTTCAATTTCTCTCATGGTACCCATGAGTATCATCAAGAAACCTTGAACAATCTCAGGGCCGCCATGCAGAATACTCAAATCCTTTGCGCCGTCATGCTCGATACCAAGGGACCAGAGATTCGAACTGGTTTCCTGAAGGATGGGAAACCTATTCAACTGAAGGAAGGTCAGGAAGTTATCATAACTACAGATTATAGCATCAAGGGGGATGAGAAAATGATCTCAATGAGTTATCAGAAGCTTGCTGTTGACTTGAAGCCAGGAAATACTATATTGTGCTCAGATGGCACCATCACCCTCACTGTCCTGTCTTGTGATGCAGTAGGTGGGACGGTGAGATGTCGTTGTGAAAACACTGCCATGTTGGGAGAAAGAAAGAATGTGAATCTTCCTGGTGTAGTGGTGGATCTTCCTACTCTGACAGATAAGGATAAAGAGGATATTCTCGATTGGGGTGTTCCCAACAGTATCGATATGATTGCTCTTTCATTTGTCCGTAAGGGTTCTGATCTAGTCAATGTCCGCAAGGTTCTTGGCCCCCATTCAAAGCATATAAAACTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAATTTTGATGAAATCTTGCGCGAGACTGATGCATTCATGGTTGCTCGAGGTGATCTTGGAATGGAAATTCCAGTTGAGAAGATCTTCTTGGCGCAGAAAATGATGATTTACAAGTGTAATCTTGCTGGCAAACCTGTGGTTACAGCCACCCAAATGCTTGAATCAATGATCAAATCTCCACGGCCAACTCGTGCAGAAGCAACAGATGTAGCTAACGCCGTTCTTGATGGCACTGATTGTGTTATGCTTAGTGGTGAGAGTGCAGCTGGGTCATATCCTGAACTTGCTGTCAAGATCATGGCTCGTATTTGTATTGAAGCAGAATCATCCTTGGACTATGGGGTCATCTTTAAGGAGATGATAAGGGCCACCCCACTTCCAATGAGCCCATTGGAGAGTCTTGCATCCTCAGCTGTTCGAACCGCTAACAAGGCAAAAGCGAAGCTCATTGTCGTGTTGACTCGTGGTGGAACCACAGCAAAGTTGGTTGCCAAGTATAGGCCAGCTGTTCCGATCCTCTCAGTCGTCGTCCCTGTTTTAACTACTGATTCTTTTGATTGGACCTGCAGCGATGAGGCCCCAGCCCGACACAGCCTGATCCACAGGGGCTTGATTCCCATACTTGCTGAAGGATCTGCAAAGGCCACTGATGCCGAATCCACAGAAGTGATACTAGAAGCTGCTCTGAAATCAGCCATGGGAAAGGGACTGTGCAATCCTGGCGACGCCATTGTTGCACTTCACCGTATCGGAGCTGCCTCCGTCATCAAGATATGCATCGTGAAGTGA

Protein sequence

MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALHRIGAASVIKICIVK
Homology
BLAST of MC09g1270 vs. ExPASy Swiss-Prot
Match: Q42806 (Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 3.0e-263
Identity = 466/511 (91.19%), Postives = 484/511 (94.72%), Query Frame = 0

Query: 1   MANIDIEGILK-ELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHE 60
           MANIDIEGILK + P DGR+PKTKIVCTLGPASRSV M EKLLRAGMNVARFNFSHGTH+
Sbjct: 1   MANIDIEGILKQQQPYDGRVPKTKIVCTLGPASRSVEMTEKLLRAGMNVARFNFSHGTHD 60

Query: 61  YHQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKG 120
           YHQETLNNL+ AM NT ILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEV ITTDY IKG
Sbjct: 61  YHQETLNNLKTAMHNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVTITTDYDIKG 120

Query: 121 DEKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVN 180
           D +MISMSY+KL V LKPGNTILCSDGTITLTVLSCD   GTVRCRCENTA LGERKNVN
Sbjct: 121 DPEMISMSYKKLPVHLKPGNTILCSDGTITLTVLSCDPDAGTVRCRCENTATLGERKNVN 180

Query: 181 LPGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMS 240
           LPGVVVDLPTLT+KDKEDIL WGVPN IDMIALSFVRKGSDLVNVRKVLGPH+K+I+LMS
Sbjct: 181 LPGVVVDLPTLTEKDKEDILGWGVPNKIDMIALSFVRKGSDLVNVRKVLGPHAKNIQLMS 240

Query: 241 KVENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQ 300
           KVENQEGV+NFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNL GKPVVTATQ
Sbjct: 241 KVENQEGVLNFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLVGKPVVTATQ 300

Query: 301 MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSL 360
           MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAG+YPELAVKIMARICIEAESSL
Sbjct: 301 MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMARICIEAESSL 360

Query: 361 DYGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVP 420
           DYG IFKEMIR+TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGG+TAKLVAKYRPAVP
Sbjct: 361 DYGAIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGSTAKLVAKYRPAVP 420

Query: 421 ILSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKS 480
           ILSVVVPVL+TDSFDWTCSDE PARHSLI+RGLIPIL EGSAKATDAESTEVILEAALKS
Sbjct: 421 ILSVVVPVLSTDSFDWTCSDETPARHSLIYRGLIPILGEGSAKATDAESTEVILEAALKS 480

Query: 481 AMGKGLCNPGDAIVALHRIGAASVIKICIVK 511
           A  + LC PGDA+VALHRIGAASVIKICIVK
Sbjct: 481 ATERALCKPGDAVVALHRIGAASVIKICIVK 511

BLAST of MC09g1270 vs. ExPASy Swiss-Prot
Match: P22200 (Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 1.4e-260
Identity = 452/510 (88.63%), Postives = 489/510 (95.88%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDI GI+K+LP+DGRIPKTKIVCTLGP+SR+VPM+EKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIAGIMKDLPNDGRIPKTKIVCTLGPSSRTVPMLEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETL+NL+ AMQNTQILCAVMLDTKGPEIRTGFL DGKPIQLKEGQE+ ++TDY+IKG+
Sbjct: 61  HQETLDNLKIAMQNTQILCAVMLDTKGPEIRTGFLTDGKPIQLKEGQEITVSTDYTIKGN 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSY+KL +DLKPGNTILC+DGTITLTVLSCD   GTVRCRCENTA LGERKNVNL
Sbjct: 121 EEMISMSYKKLVMDLKPGNTILCADGTITLTVLSCDPPSGTVRCRCENTATLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLT+KDKEDIL+WGVPN+IDMIALSFVRKGSDLVNVRKVLGPH+K I+LMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILEWGVPNNIDMIALSFVRKGSDLVNVRKVLGPHAKRIQLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETD+FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGK VVTATQM
Sbjct: 241 VENQEGVINFDEILRETDSFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKAVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSP PTRAEATDVANAVLDGTDCVMLSGESAAG+YPELAVKIM+RICIEAESSLD
Sbjct: 301 LESMIKSPAPTRAEATDVANAVLDGTDCVMLSGESAAGAYPELAVKIMSRICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
              IFKEMIR TPLPMSPLESLASSAVRTANKA+AKLIVVLTRGG+TAKLVAKYRPAVPI
Sbjct: 361 NEAIFKEMIRCTPLPMSPLESLASSAVRTANKARAKLIVVLTRGGSTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDW+ SDE PARHSL++RGLIP+L EGSAKATD+ESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWSISDETPARHSLVYRGLIPLLGEGSAKATDSESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 511
           + +GLC PGDA+VALHRIG+ASVIKIC+VK
Sbjct: 481 VTRGLCKPGDAVVALHRIGSASVIKICVVK 510

BLAST of MC09g1270 vs. ExPASy Swiss-Prot
Match: Q42954 (Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 4.0e-223
Identity = 388/491 (79.02%), Postives = 438/491 (89.21%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAMQNTQILC 79
           PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHG+H+YHQET++NLR AM++T ILC
Sbjct: 18  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGSHDYHQETIDNLRQAMESTGILC 77

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKD KP+QLK+GQE+ I+TDYSIKGDE MI MSY+KLA D+KP +
Sbjct: 78  AVMLDTKGPEIRTGFLKDAKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKPQS 137

Query: 140 TILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDIL 199
            ILC+DG IT TVLSCD   G  RCRCENTA+LGERKNVNLPGV+VDLPTLTDKDK+DIL
Sbjct: 138 VILCADGQITFTVLSCDKENGLDRCRCENTAVLGERKNVNLPGVIVDLPTLTDKDKDDIL 197

Query: 200 DWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDA 259
           +WGVPN IDMIALSFVRKGSDLV VRK+LG H+K+I LMSKVENQEGV NFD+IL  +DA
Sbjct: 198 NWGVPNHIDMIALSFVRKGSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDDILLNSDA 257

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FMVARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 258 FMVARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 317

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YP+LAV  MA+ICIEAES++DY  +FK ++   P+PMSPL
Sbjct: 318 NAVLDGTDCVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPDVFKRIMSNAPVPMSPL 377

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSD 439
           ESLASSAVRTAN AKA LI+VLTRGG+TAKLVAKYRP +PILSVVVP + TDSFDWTCSD
Sbjct: 378 ESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSVVVPEIKTDSFDWTCSD 437

Query: 440 EAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALHRIG 499
           E+PARHSLI RGL+P+L  GSA+A+  ESTE  L+ AL+ A  KGLC  GD++VALHR+G
Sbjct: 438 ESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTKGLCKQGDSVVALHRVG 497

Query: 500 AASVIKICIVK 511
            ASVIKI  VK
Sbjct: 498 TASVIKIVTVK 508

BLAST of MC09g1270 vs. ExPASy Swiss-Prot
Match: O65595 (Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g26390 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 8.0e-216
Identity = 378/491 (76.99%), Postives = 431/491 (87.78%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAMQNTQILC 79
           PKTKIVCTLGPASRSVPM+EKLL AGM+VARFNFSHG++EYHQETL+NLR AM NT +LC
Sbjct: 7   PKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLC 66

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKDGKPIQLK+GQE+ I+TDY +KGDEK I MSY+KLA D+ PG 
Sbjct: 67  AVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPGM 126

Query: 140 TILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDIL 199
            ILC+DGTI+L VLSCD   GTVRCRCENT+MLGERKNVNLPGVVVDLPTLT+KDK+DIL
Sbjct: 127 VILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEKDKQDIL 186

Query: 200 DWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDA 259
           +WGVPN IDMIALSFVRKGSDLV VRK+LG H+K I LMSKVENQEGV NFD+IL  +DA
Sbjct: 187 EWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDILINSDA 246

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FM+ARGDLGMEIP+EKIFLAQK+MIYKCN  GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 247 FMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRAEATDVA 306

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YPELAV+ MA+IC+EAES+LDYG IFK ++    +PMSP+
Sbjct: 307 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAAVPMSPM 366

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSD 439
           ESLASSAVRTA  ++A L++VLTRGG+TA+LVAKYRP +PILSVVVP +T+DSFDW CS+
Sbjct: 367 ESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSN 426

Query: 440 EAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALHRIG 499
           EAPARHSLI+RGL+P+L  GSA+A+  ESTE  LE A +    K LC  GD++VAL R G
Sbjct: 427 EAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVVALFRTG 486

Query: 500 AASVIKICIVK 511
            A VIKI  VK
Sbjct: 487 NAIVIKILTVK 497

BLAST of MC09g1270 vs. ExPASy Swiss-Prot
Match: O44006 (Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.5e-124
Identity = 241/496 (48.59%), Postives = 330/496 (66.53%), Query Frame = 0

Query: 21  KTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAM-QNTQILC 80
           +TKIVCT+GP+   V  + +L+ AGMNV R NFSHG HE H   + NL+ A+ Q      
Sbjct: 57  RTKIVCTMGPSCWDVDKMVQLIDAGMNVCRLNFSHGDHEAHGRVVKNLQEALKQRPGKRV 116

Query: 81  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGN 140
           A++LDTKGPEIRTG L+  KPI+L  G  + I TDYS  G++  I+ SY+KL   +KPGN
Sbjct: 117 ALLLDTKGPEIRTGMLEGDKPIELHAGDMLKIVTDYSFVGNKSCIACSYEKLPSSVKPGN 176

Query: 141 TILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDIL 200
           TIL +DG++++ V+ C      V  R  N A++G +KN+NLPGV VDLP + +KDK DIL
Sbjct: 177 TILIADGSLSVEVVECGK--DYVMTRVMNPAIIGNKKNMNLPGVKVDLPVIGEKDKNDIL 236

Query: 201 DWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDA 260
           ++G+P   + IA SFV+   D+  +R +LG   ++IK++ K+EN EG++NFDEIL+E D 
Sbjct: 237 NFGIPMGCNFIAASFVQSADDVRYIRSILGTKGRNIKIIPKIENVEGLLNFDEILQEADG 296

Query: 261 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 320
            M+ARGDLGMEIP EK+FLAQKMMI KCN+AGKPV+TATQMLESM K+PRPTRAEA DVA
Sbjct: 297 IMIARGDLGMEIPPEKVFLAQKMMISKCNVAGKPVITATQMLESMTKNPRPTRAEAADVA 356

Query: 321 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPL 380
           NAVLDGTDCVMLSGE+A GS+P  AV +M+R+C EAE  +DY  +F+   +AT  P+   
Sbjct: 357 NAVLDGTDCVMLSGETANGSFPVQAVTVMSRVCFEAEGCIDYQQVFRATCQATMTPIDTQ 416

Query: 381 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSD 440
           E++A +AV TA    A LI+ LT  G TA+L+AKYRP  PIL++              + 
Sbjct: 417 EAVARAAVETAQSINASLILALTETGRTARLIAKYRPMQPILAL-------------SAS 476

Query: 441 EAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALHRI- 500
           E   +   + RG+   L          + T+ ++  AL +A    L + GD+IVA+H I 
Sbjct: 477 EETIKQLQVIRGVTTFL------VPTFQGTDQLIRNALSAAKELQLVSEGDSIVAVHGIK 531

Query: 501 ----GAASVIKICIVK 511
               G ++++K+ +V+
Sbjct: 537 EEVAGWSNLLKVLVVE 531

BLAST of MC09g1270 vs. NCBI nr
Match: XP_022138945.1 (pyruvate kinase, cytosolic isozyme [Momordica charantia])

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL
Sbjct: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK
Sbjct: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLCNPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. NCBI nr
Match: XP_038892449.1 (pyruvate kinase, cytosolic isozyme [Benincasa hispida] >XP_038892452.1 pyruvate kinase, cytosolic isozyme [Benincasa hispida])

HSP 1 Score: 961 bits (2484), Expect = 0.0
Identity = 492/510 (96.47%), Postives = 501/510 (98.24%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN+ILCSDGTITLTVLSCD   G V CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNSILCSDGTITLTVLSCDPEAGRVICRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PG+VVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGVIFKEMIR+TPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVIFKEMIRSTPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. NCBI nr
Match: XP_008445909.1 (PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0034093.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa] >TYK15825.1 pyruvate kinase, cytosolic isozyme [Cucumis melo var. makuwa])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 490/510 (96.08%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPLESLASSAVRTANKA+AKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKARAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. NCBI nr
Match: XP_004147096.1 (pyruvate kinase, cytosolic isozyme [Cucumis sativus] >XP_031741666.1 pyruvate kinase, cytosolic isozyme [Cucumis sativus] >KGN51576.1 hypothetical protein Csa_008390 [Cucumis sativus])

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 490/510 (96.08%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PG+VVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. NCBI nr
Match: AKN59232.1 (pyruvate kinase [Cucumis sativus])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 487/510 (95.49%), Postives = 496/510 (97.25%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKN  L
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNXXL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PG+VVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPL SLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLXSLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. ExPASy TrEMBL
Match: A0A6J1CAX5 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111009998 PE=3 SV=1)

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL
Sbjct: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK
Sbjct: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLCNPGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. ExPASy TrEMBL
Match: A0A5A7SUF8 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003300 PE=3 SV=1)

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 490/510 (96.08%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPLESLASSAVRTANKA+AKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKARAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. ExPASy TrEMBL
Match: A0A0A0KV32 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_5G580610 PE=3 SV=1)

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 490/510 (96.08%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PG+VVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. ExPASy TrEMBL
Match: A0A1S3BDT2 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103488792 PE=3 SV=1)

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 490/510 (96.08%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKNVNL
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGVVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKILGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPLESLASSAVRTANKA+AKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLESLASSAVRTANKARAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. ExPASy TrEMBL
Match: A0A218KBQ1 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=PK32 PE=2 SV=1)

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 487/510 (95.49%), Postives = 496/510 (97.25%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           MANIDIEGILKELP+DGRIPKTKIVCTLGPASRSV MIEKLLRAGMNVARFNFSHGTHEY
Sbjct: 1   MANIDIEGILKELPNDGRIPKTKIVCTLGPASRSVTMIEKLLRAGMNVARFNFSHGTHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETLNNLR AMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD
Sbjct: 61  HQETLNNLRTAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E+MISMSYQKLAVDLKPGN ILCSDGTITLTVLSCD   G V CRCENTAMLGERKN  L
Sbjct: 121 EEMISMSYQKLAVDLKPGNNILCSDGTITLTVLSCDPEAGRVVCRCENTAMLGERKNXXL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PG+VVDLPTLT+KDKEDIL WGVPNSIDMIALSFVRKGSDLV VRK+LGPH+KHIKLMSK
Sbjct: 181 PGIVVDLPTLTEKDKEDILGWGVPNSIDMIALSFVRKGSDLVTVRKLLGPHAKHIKLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPE+AVKIMARICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPEIAVKIMARICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           YGV+FKEMIRATPLPMSPL SLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YGVVFKEMIRATPLPMSPLXSLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPVLTTDSFDWTCSDE+PARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA
Sbjct: 421 LSVVVPVLTTDSFDWTCSDESPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 510
           MGKGLC PGDAIVALHRIGAASVIKICIVK
Sbjct: 481 MGKGLCKPGDAIVALHRIGAASVIKICIVK 510

BLAST of MC09g1270 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 891.0 bits (2301), Expect = 4.6e-259
Identity = 449/510 (88.04%), Postives = 485/510 (95.10%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           M+NIDIEGILKELP+DGRIPKTKIVCTLGPASR+V MIEKLL+AGMNVARFNFSHG+HEY
Sbjct: 1   MSNIDIEGILKELPNDGRIPKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETL+NLR+AM NT IL AVMLDTKGPEIRTGFLKDG PIQLKEGQE+ ITTDY I+GD
Sbjct: 61  HQETLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           E  ISMSY+KL +D+KPGNTILC+DG+I+L VLSCD   GTVRCRCEN+AMLGERKNVNL
Sbjct: 121 ESTISMSYKKLPLDVKPGNTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLTDKD EDIL WGVPNSIDMIALSFVRKGSDLVNVRKVLG H+K I LMSK
Sbjct: 181 PGVVVDLPTLTDKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGVINFDEILRETDAFMVARGDLGMEIP+EKIFLAQK+MIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAG+YPE+AVK+MA+ICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           Y  IFKEMIRATPLPMSPLESLASSAVRTANKA+AKLI+VLTRGG+TA LVAKYRPAVPI
Sbjct: 361 YNTIFKEMIRATPLPMSPLESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPV+TTDSFDW+CSDE+PARHSLI+RGLIP+LAEGSAKATD+E+TEVI+EAALKSA
Sbjct: 421 LSVVVPVMTTDSFDWSCSDESPARHSLIYRGLIPMLAEGSAKATDSEATEVIIEAALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 511
             +GLCN GDAIVALHRIGAASVIKIC+VK
Sbjct: 481 TQRGLCNRGDAIVALHRIGAASVIKICVVK 510

BLAST of MC09g1270 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-257
Identity = 449/510 (88.04%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 1   MANIDIEGILKELPSDGRIPKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEY 60
           M+NIDIEGILKELP+DGR PKTKIVCTLGPASRSV MIEKLL+AGMNVARFNFSHG+HEY
Sbjct: 1   MSNIDIEGILKELPNDGRTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEY 60

Query: 61  HQETLNNLRAAMQNTQILCAVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGD 120
           HQETL+NLR AMQNT IL AVMLDTKGPEIRTGFLKDG PIQLKEGQE+ ITTDY IKGD
Sbjct: 61  HQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIKGD 120

Query: 121 EKMISMSYQKLAVDLKPGNTILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNL 180
           EK ISMSY+KL VD+KPGNTILC+DG+I+L V+SCD   GTV CRCENTAMLGERKNVNL
Sbjct: 121 EKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL 180

Query: 181 PGVVVDLPTLTDKDKEDILDWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSK 240
           PGVVVDLPTLTDKD EDIL WGVPN+IDMIALSFVRKGSDLVNVRKVLG HSK I LMSK
Sbjct: 181 PGVVVDLPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSKSIMLMSK 240

Query: 241 VENQEGVINFDEILRETDAFMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQM 300
           VENQEGV+NFDEILRETDAFMVARGDLGMEIP+EKIFLAQKMMIYKCNLAGKPVVTATQM
Sbjct: 241 VENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQM 300

Query: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLD 360
           LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAG+YPE+AVK MA+ICIEAESSLD
Sbjct: 301 LESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSLD 360

Query: 361 YGVIFKEMIRATPLPMSPLESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPI 420
           Y  IFKEMIRATPLPMS LESLASSAVRTANKAKAKLI+VLTRGGTTAKLVAKYRPAVPI
Sbjct: 361 YNTIFKEMIRATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPI 420

Query: 421 LSVVVPVLTTDSFDWTCSDEAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSA 480
           LSVVVPV T+D+F+W+CSDE+PARHSLI+RGLIP+L EGSAKATD+ESTE I+E+ALKSA
Sbjct: 421 LSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTEEIIESALKSA 480

Query: 481 MGKGLCNPGDAIVALHRIGAASVIKICIVK 511
             KGLCN GDA+VALHRIGAASVIKIC+VK
Sbjct: 481 TEKGLCNHGDAVVALHRIGAASVIKICVVK 510

BLAST of MC09g1270 vs. TAIR 10
Match: AT5G56350.1 (Pyruvate kinase family protein )

HSP 1 Score: 767.7 bits (1981), Expect = 5.9e-222
Identity = 385/491 (78.41%), Postives = 435/491 (88.59%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAMQNTQILC 79
           PKTKIVCTLGPASRSVPM+EKLLRAGMNVARFNFSHG+HEYHQETL+NL  AM NT ILC
Sbjct: 8   PKTKIVCTLGPASRSVPMVEKLLRAGMNVARFNFSHGSHEYHQETLDNLHQAMLNTGILC 67

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKDGKPIQLK+GQE+ I+TDY +KGDE  I MSY+KLAVD+ PG 
Sbjct: 68  AVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDENTICMSYKKLAVDVNPGM 127

Query: 140 TILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDIL 199
            ILC+DGTI+L VLSCD   GTVRCRCEN+AMLGERKNVNLPGVVVDLPTLT+KDKEDI+
Sbjct: 128 VILCADGTISLLVLSCDKENGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTEKDKEDIM 187

Query: 200 DWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDA 259
            WGVPN IDMIALSFVRKGSDLV VRK+LG H+K+I LMSKVENQEGV NFD+IL  +DA
Sbjct: 188 QWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKNILLMSKVENQEGVANFDDILVNSDA 247

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FM+ARGDLGMEIP+EKIFLAQK+MIYKCN+ GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 248 FMIARGDLGMEIPIEKIFLAQKVMIYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVA 307

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YPELAV+ MA+IC+EAES+LDYG +FK ++  +P+PMSPL
Sbjct: 308 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDVFKRIMLYSPVPMSPL 367

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSD 439
           ESLASSAVRTAN A+A LI+VLTRGG+TA+LVAKYRP +PILSVVVP + TD FDW+CSD
Sbjct: 368 ESLASSAVRTANSARATLIMVLTRGGSTARLVAKYRPGMPILSVVVPEIKTDFFDWSCSD 427

Query: 440 EAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALHRIG 499
           E+PARHSLI RGLIP+L  GSA+A+  ESTE  +E A +    K LC  GD++VAL R+G
Sbjct: 428 ESPARHSLIFRGLIPVLYAGSARASHDESTEEAIEFATQYGKEKELCKTGDSVVALLRVG 487

Query: 500 AASVIKICIVK 511
            ASVIKI  VK
Sbjct: 488 NASVIKILTVK 498

BLAST of MC09g1270 vs. TAIR 10
Match: AT4G26390.1 (Pyruvate kinase family protein )

HSP 1 Score: 751.1 bits (1938), Expect = 5.7e-217
Identity = 378/491 (76.99%), Postives = 431/491 (87.78%), Query Frame = 0

Query: 20  PKTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAMQNTQILC 79
           PKTKIVCTLGPASRSVPM+EKLL AGM+VARFNFSHG++EYHQETL+NLR AM NT +LC
Sbjct: 7   PKTKIVCTLGPASRSVPMVEKLLMAGMSVARFNFSHGSYEYHQETLDNLRQAMLNTGMLC 66

Query: 80  AVMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGN 139
           AVMLDTKGPEIRTGFLKDGKPIQLK+GQE+ I+TDY +KGDEK I MSY+KLA D+ PG 
Sbjct: 67  AVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDLKGDEKTICMSYKKLAQDVNPGM 126

Query: 140 TILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDIL 199
            ILC+DGTI+L VLSCD   GTVRCRCENT+MLGERKNVNLPGVVVDLPTLT+KDK+DIL
Sbjct: 127 VILCADGTISLKVLSCDKEKGTVRCRCENTSMLGERKNVNLPGVVVDLPTLTEKDKQDIL 186

Query: 200 DWGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDA 259
           +WGVPN IDMIALSFVRKGSDLV VRK+LG H+K I LMSKVENQEGV NFD+IL  +DA
Sbjct: 187 EWGVPNQIDMIALSFVRKGSDLVQVRKLLGKHAKTILLMSKVENQEGVANFDDILINSDA 246

Query: 260 FMVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVA 319
           FM+ARGDLGMEIP+EKIFLAQK+MIYKCN  GKPVVTATQMLESMIKSPRPTRAEATDVA
Sbjct: 247 FMIARGDLGMEIPIEKIFLAQKVMIYKCNFMGKPVVTATQMLESMIKSPRPTRAEATDVA 306

Query: 320 NAVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPL 379
           NAVLDGTDCVMLSGE+AAG+YPELAV+ MA+IC+EAES+LDYG IFK ++    +PMSP+
Sbjct: 307 NAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGDIFKRIMLHAAVPMSPM 366

Query: 380 ESLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLTTDSFDWTCSD 439
           ESLASSAVRTA  ++A L++VLTRGG+TA+LVAKYRP +PILSVVVP +T+DSFDW CS+
Sbjct: 367 ESLASSAVRTATSSRATLMMVLTRGGSTARLVAKYRPGIPILSVVVPEITSDSFDWACSN 426

Query: 440 EAPARHSLIHRGLIPILAEGSAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALHRIG 499
           EAPARHSLI+RGL+P+L  GSA+A+  ESTE  LE A +    K LC  GD++VAL R G
Sbjct: 427 EAPARHSLIYRGLVPVLYAGSARASIDESTEETLEFASEYGKKKQLCKTGDSVVALFRTG 486

Query: 500 AASVIKICIVK 511
            A VIKI  VK
Sbjct: 487 NAIVIKILTVK 497

BLAST of MC09g1270 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 675.6 bits (1742), Expect = 3.1e-194
Identity = 345/494 (69.84%), Postives = 416/494 (84.21%), Query Frame = 0

Query: 21  KTKIVCTLGPASRSVPMIEKLLRAGMNVARFNFSHGTHEYHQETLNNLRAAMQNTQILCA 80
           KTKIVCTLGPASRSV M+EKLL+AGMNVARFNFSHG+H YHQETL+NLR AM+NT I CA
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCA 76

Query: 81  VMLDTKGPEIRTGFLKDGKPIQLKEGQEVIITTDYSIKGDEKMISMSYQKLAVDLKPGNT 140
           VMLDTKGPEIRTGFLK+GKP++L +GQE+ I+TDY+++GD   ISMSY+KLA DLK G+ 
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPVELIQGQEITISTDYTMEGDSNTISMSYKKLAEDLKSGDV 136

Query: 141 ILCSDGTITLTVLSCDAVGGTVRCRCENTAMLGERKNVNLPGVVVDLPTLTDKDKEDILD 200
           ILCSDGTI+LTVLSCD   G VR RCEN+A+LGERKNVNLPG+VVDLPTLT+KD+EDIL 
Sbjct: 137 ILCSDGTISLTVLSCDKNLGLVRARCENSAVLGERKNVNLPGIVVDLPTLTEKDQEDILQ 196

Query: 201 WGVPNSIDMIALSFVRKGSDLVNVRKVLGPHSKHIKLMSKVENQEGVINFDEILRETDAF 260
           WGVPN ID+IALSFVRKGSDLV VRK+LG ++K I LMSKVENQEGV+NFD+IL  +DAF
Sbjct: 197 WGVPNKIDIIALSFVRKGSDLVEVRKLLGENAKSIMLMSKVENQEGVMNFDKILEYSDAF 256

Query: 261 MVARGDLGMEIPVEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPTRAEATDVAN 320
           MVARGDLGMEIP+EK+FLAQKMMI K N  GKP+VTATQMLESM KSPRPTRAEATDVAN
Sbjct: 257 MVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRPTRAEATDVAN 316

Query: 321 AVLDGTDCVMLSGESAAGSYPELAVKIMARICIEAESSLDYGVIFKEMIRATPLPMSPLE 380
           AVLDGTDCVMLSGE+AAG++PE AV  M+RIC EAE  +DY  + K++     LP+SP+E
Sbjct: 317 AVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTMHKKIQDIVSLPLSPIE 376

Query: 381 SLASSAVRTANKAKAKLIVVLTRGGTTAKLVAKYRPAVPILSVVVPVLT-TDSFDWTCSD 440
           SLA+SAV TA    A  IVVLT+GG T +LVAKYRP+VPILSV+VP +T TD F+W+CS+
Sbjct: 377 SLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVIVPEITRTDDFEWSCSE 436

Query: 441 EAP--ARHSLIHRGLIPILAEG-SAKATDAESTEVILEAALKSAMGKGLCNPGDAIVALH 500
            A   AR  LI+RG++P++A G SA++++ +STE  ++ A++ A  KG+C  GD+IVALH
Sbjct: 437 SAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEFAKKKGICKTGDSIVALH 496

Query: 501 RIGAASVIKICIVK 511
           +I  +SV+KI  V+
Sbjct: 497 KIDGSSVVKILNVE 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q428063.0e-26391.19Pyruvate kinase, cytosolic isozyme OS=Glycine max OX=3847 PE=2 SV=1[more]
P222001.4e-26088.63Pyruvate kinase, cytosolic isozyme OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q429544.0e-22379.02Pyruvate kinase, cytosolic isozyme OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O655958.0e-21676.99Probable pyruvate kinase, cytosolic isozyme OS=Arabidopsis thaliana OX=3702 GN=A... [more]
O440062.5e-12448.59Pyruvate kinase OS=Eimeria tenella OX=5802 GN=PYK PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022138945.10.0100.00pyruvate kinase, cytosolic isozyme [Momordica charantia][more]
XP_038892449.10.096.47pyruvate kinase, cytosolic isozyme [Benincasa hispida] >XP_038892452.1 pyruvate ... [more]
XP_008445909.10.096.08PREDICTED: pyruvate kinase, cytosolic isozyme [Cucumis melo] >KAA0034093.1 pyruv... [more]
XP_004147096.10.096.08pyruvate kinase, cytosolic isozyme [Cucumis sativus] >XP_031741666.1 pyruvate ki... [more]
AKN59232.10.095.49pyruvate kinase [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1CAX50.0100.00Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111009998 PE=3 SV=1[more]
A0A5A7SUF80.096.08Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00330... [more]
A0A0A0KV320.096.08Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_5G580610 PE=3 SV=1[more]
A0A1S3BDT20.096.08Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103488792 PE=3 SV=1[more]
A0A218KBQ10.095.49Pyruvate kinase OS=Cucumis sativus OX=3659 GN=PK32 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08570.14.6e-25988.04Pyruvate kinase family protein [more]
AT5G63680.11.1e-25788.04Pyruvate kinase family protein [more]
AT5G56350.15.9e-22278.41Pyruvate kinase family protein [more]
AT4G26390.15.7e-21776.99Pyruvate kinase family protein [more]
AT3G04050.13.1e-19469.84Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 77..93
score: 62.9
coord: 333..349
score: 54.03
coord: 264..288
score: 64.86
coord: 314..332
score: 77.25
coord: 289..313
score: 74.58
coord: 237..263
score: 52.54
coord: 207..221
score: 51.79
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 21..497
e-value: 2.7E-156
score: 518.8
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 1..510
IPR001697Pyruvate kinaseCDDcd00288Pyruvate_Kinasecoord: 19..509
e-value: 0.0
score: 731.038
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 20..361
e-value: 1.5E-158
score: 527.3
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 21..355
e-value: 6.9E-197
score: 656.6
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 88..188
e-value: 6.9E-197
score: 656.6
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 380..502
e-value: 9.7E-22
score: 77.4
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 356..497
e-value: 6.9E-197
score: 656.6
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 344..509
NoneNo IPR availablePANTHERPTHR11817:SF87PYRUVATE KINASEcoord: 1..510
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 235..247
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 18..361
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 88..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1270.1MC09g1270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity