MC09g1251 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1251
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein FAR1-RELATED SEQUENCE
LocationMC09: 18632568 .. 18634123 (-)
RNA-Seq ExpressionMC09g1251
SyntenyMC09g1251
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCAAAAATCCATGGCCGCTGCCGCCGGAAGCTGTTCTTCATACAAGAAATACGAGATCCGCCATAGACTTCCGGATCCCAAAATCGCCGCCCTCTTAGTCATCGATATGCAGAACTACTTCTCCGCCATGGCCAAGCCCATCCTCCCCAATGTCCTCGCCACCATCCGCCTCTGCCGCGCCGCCTCCATCCCCGTCATCTTCACCCGCCACGCCCACTCCTCTCCCTCCGACAACCCCATTCTCGGCGAGTGGTGGAACGGCGACATCATCCTCGACGGCACCCCCGCCGCCGACCTCATCCCCGATCTCGGCCCGGTCGCCGCTGACGAGGTCATCCGGAAGAACACCTACTCCGCGTTCAGGAACACGCCGCTGGAGGACTGCTTGAGAGCGAAGGGCGTGGAGGAGGTGATTGTGACTGGGGTTATGACCAACGTCTGCTGCGAGACGACGGCGCGTGAGGCGTTTGTGAGGGGCTACAGAGTGTTCTTCTCGACGGACGCGACGGCGACGGCGGACGAGGACCTGCACGAGGCTACGCTGAAGACTTTGGCTTATGGATTTGCTTACTTGGTGGATCGCCAGAGGCTGGAAGAACTTTCTGGTAATTGAACACCAAATTAGGCACTTATTATGCTAATCTTCTGTTTACTAGAAATTTAGAATCTCTGGAATTTTACCGTGCCAAATATGTATAGCTTTGTTTTTGGTTCTGTCTTTTAGCTATTGGGTTTTCTTTTTCTTCTTCTTGAAGACATGGGGAAGATGGAAGATGCAAACTTACATGGGCAAGTGTGTAATAGTTCTGAGTTTGATTTGGAAGATAATATAGAGTCTAGGATCAATACTGAACTTGAAATTATGGAGCCAGGAATTGGGATGAAGTTTGGTTCTATTGAGGAAGCATATCAGTTCTATGTCAATTATGGAGTGAAAGTTGGTTTTAGTGTAAGGAAAAACCATCGTAAGAAGAAAAAGAATGGAGTTATTTCTAGAGCAGTTTTTAAAGAGGGGTTTCGCCAAGTAGACAAACGAAGAGAAAATGTTTTGTTTACTCGGCCAATTTCAAGAACAGGGTGCCAAGCTCAGTTAACATTCCTGCATGGAAAGAATGGTCAATACAAAGTACTATCTTTTAATAGAAATTAAAATCATGATCTCAGTTAACATGCCTGCTTGGAAAGAATGGTCAGTACTGTCTTTTAATAGAAATTACAATCATGATCTTGGTAGAACACGAATGAAGTATGCTTTAAGTGTCAATGGGAACGTTTCCAACACTAAAAAAGTTTATGATGCTGACGGATCAAGAAGTTCATCTAATACAACCATGGAATTTATGAGAAGGAAAGTTGGTGGTGTAGAAAATTTGGAAAATTATACACACAGAAAAAAACAAGATTTGGAAAGGAGAGGAAGCTACATCACTCTTACAGTATTTTAGAGAAAGACAAAAGGGTGGGATCCTTCTTTTTTTTTTTTTTTTTAATGCATTTCAACTTGATCAAGATGACAAGATCGTTAATATTTTTTGGACAGATGCACGTTCTATT

mRNA sequence

AACCAAAAATCCATGGCCGCTGCCGCCGGAAGCTGTTCTTCATACAAGAAATACGAGATCCGCCATAGACTTCCGGATCCCAAAATCGCCGCCCTCTTAGTCATCGATATGCAGAACTACTTCTCCGCCATGGCCAAGCCCATCCTCCCCAATGTCCTCGCCACCATCCGCCTCTGCCGCGCCGCCTCCATCCCCGTCATCTTCACCCGCCACGCCCACTCCTCTCCCTCCGACAACCCCATTCTCGGCGAGTGGTGGAACGGCGACATCATCCTCGACGGCACCCCCGCCGCCGACCTCATCCCCGATCTCGGCCCGGTCGCCGCTGACGAGGTCATCCGGAAGAACACCTACTCCGCGTTCAGGAACACGCCGCTGGAGGACTGCTTGAGAGCGAAGGGCGTGGAGGAGGTGATTGTGACTGGGGTTATGACCAACGTCTGCTGCGAGACGACGGCGCGTGAGGCGTTTGTGAGGGGCTACAGAGTGTTCTTCTCGACGGACGCGACGGCGACGGCGGACGAGGACCTGCACGAGGCTACGCTGAAGACTTTGGCTTATGGATTTGCTTACTTGGTGGATCGCCAGAGGCTGGAAGAACTTTCTGATGCAAACTTACATGGGCAAGTGTGTAATAGTTCTGAGTTTGATTTGGAAGATAATATAGAGTCTAGGATCAATACTGAACTTGAAATTATGGAGCCAGGAATTGGGATGAAGTTTGGTTCTATTGAGGAAGCATATCAGTTCTATGTCAATTATGGAGTGAAAGTTGGTTTTAGTGTAAGGAAAAACCATCGTAAGAAGAAAAAGAATGGAGTTATTTCTAGAGCAGTTTTTAAAGAGGGGTTTCGCCAAGTAGACAAACGAAGAGAAAATGTTTTGTTTACTCGGCCAATTTCAAGAACAGGGTGCCAAGCTCAGTTAACATTCCTGCATGGAAAGAATGTTAACATGCCTGCTTGGAAAGAATGGTCAGTACTGTCTTTTAATAGAAATTACAATCATGATCTTGGTAGAACACGAATGAAGTATGCTTTAAGTGTCAATGGGAACGTTTCCAACACTAAAAAAGTTTATGATGCTGACGGATCAAGAAGTTCATCTAATACAACCATGGAATTTATGAGAAGGAAAGTTGGTGGTGTAGAAAATTTGGAAAATTATACACACAGAAAAAAACAAGATTTGGAAAGGGAAGCTACATCACTCTTACAGTATTTTAGAGAAAGACAAAAGGGTGGGATCCTTCTTTTTTTTTTTTTTTTTAATGCATTTCAACTTGATCAAGATGACAAGATCGTTAATATTTTTTGGACAGATGCACGTTCTATT

Coding sequence (CDS)

AACCAAAAATCCATGGCCGCTGCCGCCGGAAGCTGTTCTTCATACAAGAAATACGAGATCCGCCATAGACTTCCGGATCCCAAAATCGCCGCCCTCTTAGTCATCGATATGCAGAACTACTTCTCCGCCATGGCCAAGCCCATCCTCCCCAATGTCCTCGCCACCATCCGCCTCTGCCGCGCCGCCTCCATCCCCGTCATCTTCACCCGCCACGCCCACTCCTCTCCCTCCGACAACCCCATTCTCGGCGAGTGGTGGAACGGCGACATCATCCTCGACGGCACCCCCGCCGCCGACCTCATCCCCGATCTCGGCCCGGTCGCCGCTGACGAGGTCATCCGGAAGAACACCTACTCCGCGTTCAGGAACACGCCGCTGGAGGACTGCTTGAGAGCGAAGGGCGTGGAGGAGGTGATTGTGACTGGGGTTATGACCAACGTCTGCTGCGAGACGACGGCGCGTGAGGCGTTTGTGAGGGGCTACAGAGTGTTCTTCTCGACGGACGCGACGGCGACGGCGGACGAGGACCTGCACGAGGCTACGCTGAAGACTTTGGCTTATGGATTTGCTTACTTGGTGGATCGCCAGAGGCTGGAAGAACTTTCTGATGCAAACTTACATGGGCAAGTGTGTAATAGTTCTGAGTTTGATTTGGAAGATAATATAGAGTCTAGGATCAATACTGAACTTGAAATTATGGAGCCAGGAATTGGGATGAAGTTTGGTTCTATTGAGGAAGCATATCAGTTCTATGTCAATTATGGAGTGAAAGTTGGTTTTAGTGTAAGGAAAAACCATCGTAAGAAGAAAAAGAATGGAGTTATTTCTAGAGCAGTTTTTAAAGAGGGGTTTCGCCAAGTAGACAAACGAAGAGAAAATGTTTTGTTTACTCGGCCAATTTCAAGAACAGGGTGCCAAGCTCAGTTAACATTCCTGCATGGAAAGAATGTTAACATGCCTGCTTGGAAAGAATGGTCAGTACTGTCTTTTAATAGAAATTACAATCATGATCTTGGTAGAACACGAATGAAGTATGCTTTAAGTGTCAATGGGAACGTTTCCAACACTAAAAAAGTTTATGATGCTGACGGATCAAGAAGTTCATCTAATACAACCATGGAATTTATGAGAAGGAAAGTTGGTGGTGTAGAAAATTTGGAAAATTATACACACAGAAAAAAACAAGATTTGGAAAGGGAAGCTACATCACTCTTACAGTATTTTAGAGAAAGACAAAAGGGTGGGATCCTTCTTTTTTTTTTTTTTTTTAATGCATTTCAACTTGATCAAGATGACAAGATCGTTAATATTTTTTGGACAGATGCACGTTCTATT

Protein sequence

NQKSMAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEFDLEDNIESRINTELEIMEPGIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVFKEGFRQVDKRRENVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGNVSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLENYTHRKKQDLEREATSLLQYFRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARSI
Homology
BLAST of MC09g1251 vs. ExPASy Swiss-Prot
Match: Q9FMX7 (Nicotinamidase 2 OS=Arabidopsis thaliana OX=3702 GN=NIC2 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.1e-66
Identity = 125/188 (66.49%), Postives = 151/188 (80.32%), Query Frame = 0

Query: 13  SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASIPVIFTRHA 72
           SS + YE R R P+PKIAALLVIDMQN+F +MA+PIL N L TI +CR ASIPV FTRH 
Sbjct: 4   SSSRTYETRKREPNPKIAALLVIDMQNHFYSMAEPILQNALTTIDICRRASIPVFFTRHN 63

Query: 73  HSSPSDNPILGEWWNGDIILDGTPAADLIPDLG--PVAADEVIRKNTYSAFRNTPLEDCL 132
           H SP+D+ +LGEWWNGD+ILDGT  +++IP++       DE++ K+TYSAF NT L++ L
Sbjct: 64  HKSPTDHGMLGEWWNGDLILDGTTDSEIIPEINRQVTGPDEIVEKSTYSAFNNTHLQEKL 123

Query: 133 RAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLAYGFA 192
              GV+EVIV GVMTN+CCETTAREAFV+G+RVFFSTDATAT +E+LHEATL  LAYGFA
Sbjct: 124 DKIGVKEVIVIGVMTNLCCETTAREAFVKGFRVFFSTDATATVNEELHEATLMNLAYGFA 183

Query: 193 YLVDRQRL 199
           YLVD  RL
Sbjct: 184 YLVDCDRL 191

BLAST of MC09g1251 vs. ExPASy Swiss-Prot
Match: Q9FMX8 (Nicotinamidase 3 OS=Arabidopsis thaliana OX=3702 GN=NIC3 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.6e-64
Identity = 124/188 (65.96%), Postives = 152/188 (80.85%), Query Frame = 0

Query: 13  SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASIPVIFTRHA 72
           SS +KYE R R P+ KIAALLVIDMQN+FS+MAKPIL NVL TI +CR AS+PV FTRH 
Sbjct: 4   SSTRKYETRKRDPNSKIAALLVIDMQNHFSSMAKPILNNVLTTIDICRRASVPVFFTRHN 63

Query: 73  HSSPSDNPILGEWWNGDIILDGTPAADLIPDL-GPVAA-DEVIRKNTYSAFRNTPLEDCL 132
           H SP+D+ +LGEW NGD+ILDGT  +++I ++ G V   DE++ KNTYSAF  T L++ L
Sbjct: 64  HKSPTDHGMLGEWCNGDVILDGTTDSEIIQEIQGQVTGPDEMVEKNTYSAFNKTRLQENL 123

Query: 133 RAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLAYGFA 192
              GV+EVIV GVMTN+CCETTAREAF++G+RVFFSTDATAT +E+LHEATL  LA+GFA
Sbjct: 124 EKIGVKEVIVIGVMTNLCCETTAREAFIKGFRVFFSTDATATFNEELHEATLMNLAFGFA 183

Query: 193 YLVDRQRL 199
           YLVD  +L
Sbjct: 184 YLVDCDKL 191

BLAST of MC09g1251 vs. ExPASy Swiss-Prot
Match: P45743 (Isochorismatase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbB PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.7e-18
Identity = 61/178 (34.27%), Postives = 93/178 (52.25%), Query Frame = 0

Query: 24  LPDPKIAALLVIDMQNY----FSAMAKPIL---PNVLATIRLCRAASIPVIFTRHAHS-S 83
           +PDP  A LL+ DMQNY    F+A A P+     N+      C    IPV++T    S +
Sbjct: 25  VPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPGSQN 84

Query: 84  PSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNTPLEDCLRAKGV 143
           P D  +L ++W G  +  G     +I +L P   D V+ K  YSAF+ T L + +R +G 
Sbjct: 85  PDDRALLTDFW-GPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGR 144

Query: 144 EEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLAYGFAYLV 194
           +++I+TG+  ++ C  TA EAF+   + FF  DA A    + H+  L+  A   A+ V
Sbjct: 145 DQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTV 201

BLAST of MC09g1251 vs. ExPASy Swiss-Prot
Match: P0ADI5 (Enterobactin synthase component B OS=Escherichia coli O157:H7 OX=83334 GN=entB PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.1e-18
Identity = 58/169 (34.32%), Postives = 89/169 (52.66%), Query Frame = 0

Query: 26  DPKIAALLVIDMQNYFSA-------MAKPILPNVLATIRLCRAASIPVIFT-RHAHSSPS 85
           +P+ AALL+ DMQ+YF +       M + ++ N+ A    C+  +IPV +T +    S  
Sbjct: 27  EPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDE 86

Query: 86  DNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNTPLEDCLRAKGVEE 145
           D  +L + W G  +        ++  L P A D V+ K  YSAF  +PLE  L+  G  +
Sbjct: 87  DRALLNDMW-GPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQ 146

Query: 146 VIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLA 187
           +I+TGV  ++ C TTA +AF+R  + F   DA A    D H  +LK +A
Sbjct: 147 LIITGVYAHIGCMTTATDAFMRDIKPFMVADALADFSRDEHLMSLKYVA 194

BLAST of MC09g1251 vs. ExPASy Swiss-Prot
Match: P0ADI4 (Enterobactin synthase component B OS=Escherichia coli (strain K12) OX=83333 GN=entB PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.1e-18
Identity = 58/169 (34.32%), Postives = 89/169 (52.66%), Query Frame = 0

Query: 26  DPKIAALLVIDMQNYFSA-------MAKPILPNVLATIRLCRAASIPVIFT-RHAHSSPS 85
           +P+ AALL+ DMQ+YF +       M + ++ N+ A    C+  +IPV +T +    S  
Sbjct: 27  EPQRAALLIHDMQDYFVSFWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDE 86

Query: 86  DNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNTPLEDCLRAKGVEE 145
           D  +L + W G  +        ++  L P A D V+ K  YSAF  +PLE  L+  G  +
Sbjct: 87  DRALLNDMW-GPGLTRSPEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQ 146

Query: 146 VIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLA 187
           +I+TGV  ++ C TTA +AF+R  + F   DA A    D H  +LK +A
Sbjct: 147 LIITGVYAHIGCMTTATDAFMRDIKPFMVADALADFSRDEHLMSLKYVA 194

BLAST of MC09g1251 vs. NCBI nr
Match: KAA0034066.1 (nicotinamidase 2-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 545 bits (1405), Expect = 8.51e-189
Identity = 296/462 (64.07%), Postives = 351/462 (75.97%), Query Frame = 0

Query: 1   NQKSMAAAAGSC----SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATI 60
           N KS  AAA S     SSYKKYEIRHRLP+PKIAALLVIDMQNYFSAMA+PI PN+L TI
Sbjct: 3   NPKSKPAAATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPNILTTI 62

Query: 61  RLCRAASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKN 120
           RLCRAASIPVIFTRHAH+S SDNPIL EWWNGDIILDGT AA LI DL    ADEVIRKN
Sbjct: 63  RLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADEVIRKN 122

Query: 121 TYSAFRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADED 180
           TYSAFRNT LEDCL+ KGVEEVIVTGVMTN+CCETTAREAFVRGYRVFFSTDATAT D +
Sbjct: 123 TYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLE 182

Query: 181 LHEATLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEF-DLEDNIESRINTELEIMEP 240
           LHEATLK LAYGFAYLVD +RL E+S     GQ CN+SE  DLED+ ++RI+ +LEI+EP
Sbjct: 183 LHEATLKNLAYGFAYLVDCKRLGEISAMEKIGQPCNNSEVVDLEDDRKARIDPQLEIVEP 242

Query: 241 GIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVF---KEGFRQVDKRRE 300
            IGMKF S+EE YQFYVNY  KVGF+VRK + KKKKNG++SRA F   KEGFRQV+KR+E
Sbjct: 243 VIGMKFSSVEETYQFYVNYATKVGFTVRKQYHKKKKNGIVSRASFCCSKEGFRQVNKRKE 302

Query: 301 NVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGN 360
            V +TRPISRTGC+A L  L GKN       ++ V+SF  N+NHDLG+ +MK  + V+ N
Sbjct: 303 FVHYTRPISRTGCEASLACLLGKNG------QYRVVSFKGNHNHDLGKMKMKRTMDVDTN 362

Query: 361 VSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLE-------NYTH-RKKQDLER-EATS 420
           +SN +KV+DAD SR+S   TM  + ++V G E          NY    KK  +E+ E  +
Sbjct: 363 ISNAQKVHDADSSRTSFRATMGLINKEVDGREGTGFVDKDYINYVPIEKKARIEKGEVEA 422

Query: 421 LLQYFRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARSI 445
           L+QYF ++Q+    +F+    A QL++D+ + +IFWTD RS+
Sbjct: 423 LVQYFEKKQRDNSSIFY----ALQLNEDNMVASIFWTDTRSV 454

BLAST of MC09g1251 vs. NCBI nr
Match: XP_016900280.1 (PREDICTED: uncharacterized protein LOC103488764 isoform X1 [Cucumis melo] >XP_016900281.1 PREDICTED: uncharacterized protein LOC103488764 isoform X1 [Cucumis melo])

HSP 1 Score: 525 bits (1353), Expect = 4.81e-181
Identity = 288/441 (65.31%), Postives = 333/441 (75.51%), Query Frame = 0

Query: 1   NQKSMAAAAGSC----SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATI 60
           N KS  AAA S     SSYKKYEIRHRLP+PKIAALLVIDMQNYFSAMA+PI PN+L TI
Sbjct: 3   NPKSKPAAATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPNILTTI 62

Query: 61  RLCRAASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKN 120
           RLCRAASIPVIFTRHAH+S SDNPIL EWWNGDIILDGT AA LI DL    ADEVIRKN
Sbjct: 63  RLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADEVIRKN 122

Query: 121 TYSAFRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADED 180
           TYSAFRNT LEDCL+ KGVEEVIVTGVMTN+CCETTAREAFVRGYRVFFSTDATAT D +
Sbjct: 123 TYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLE 182

Query: 181 LHEATLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEF-DLEDNIESRINTELEIMEP 240
           LHEATLK LAYGFAYLVD +RL E+S     GQ CN+SE  DLED+ ++RI+ +LEI+EP
Sbjct: 183 LHEATLKNLAYGFAYLVDCKRLGEISAMEKIGQPCNNSEVVDLEDDRKARIDPQLEIVEP 242

Query: 241 GIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVF---KEGFRQVDKRRE 300
            IGMKF S+EE YQFYVNY  KVGF+VRK + KKKKNG++SRA F   KEGFRQV+KR+E
Sbjct: 243 VIGMKFSSVEETYQFYVNYATKVGFTVRKQYHKKKKNGIVSRASFCCSKEGFRQVNKRKE 302

Query: 301 NVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGN 360
            V +TRPISRTGC+A L  L GKN       ++ V+SF  N+NHDLG+ +MK  + V+ N
Sbjct: 303 FVHYTRPISRTGCEASLACLLGKNG------QYRVVSFKGNHNHDLGKMKMKRTMDVDTN 362

Query: 361 VSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLE-------NYTH-RKKQDLEREATSL 420
           +SN +KV+DAD SR+S   TM  + ++V G E          NY    KKQ+L+RE    
Sbjct: 363 ISNAQKVHDADSSRTSFRATMGLINKEVDGREGTGFVDKDYINYVPIEKKQELKREKLKH 422

Query: 421 LQYFRERQKGGILLFFFFFNA 425
           L     + +G  LLFF   N+
Sbjct: 423 LYSILRKNRGTTLLFFMHCNS 437

BLAST of MC09g1251 vs. NCBI nr
Match: XP_031741897.1 (protein FAR1-RELATED SEQUENCE 5 isoform X1 [Cucumis sativus] >KGN50450.1 hypothetical protein Csa_000454 [Cucumis sativus])

HSP 1 Score: 537 bits (1383), Expect = 7.08e-181
Identity = 289/458 (63.10%), Postives = 350/458 (76.42%), Query Frame = 0

Query: 1   NQKSMAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCR 60
           N KS  +AA    SYKKYEIRHRLP+PK+AALLVIDMQNYFSAMAKPI P++L TIRLCR
Sbjct: 3   NPKSKPSAA--IPSYKKYEIRHRLPNPKVAALLVIDMQNYFSAMAKPIFPHILTTIRLCR 62

Query: 61  AASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSA 120
           AASIPVIFTRHAH+S SDNPIL EWWNGDIIL+GTPAA LI DL    ADEVIRKNTYSA
Sbjct: 63  AASIPVIFTRHAHASASDNPILNEWWNGDIILEGTPAAGLISDLDREDADEVIRKNTYSA 122

Query: 121 FRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEA 180
           FRNT LEDCL+ KGVEEVIVTGVMTN+CCETTAR+AFVRGYRVFFSTDATAT D +LHEA
Sbjct: 123 FRNTRLEDCLKEKGVEEVIVTGVMTNICCETTARDAFVRGYRVFFSTDATATVDLELHEA 182

Query: 181 TLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEF-DLEDNIESRINTELEIMEPGIGM 240
           TLK LAYGFAYLVD +RL E+S     GQ CN+SE  D+ED+ E+RI+ +LEI+EP IGM
Sbjct: 183 TLKNLAYGFAYLVDCKRLGEISAMKKTGQPCNNSEVVDVEDDREARIDPQLEIVEPVIGM 242

Query: 241 KFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVF---KEGFRQVDKRRENVLF 300
           KF S+EE Y+FYVNY  KVGF+VRK + KKKKNG++SRA F   KEGFRQV+KR+E V +
Sbjct: 243 KFISVEETYRFYVNYATKVGFTVRKQYHKKKKNGIVSRASFCCSKEGFRQVNKRKEFVHY 302

Query: 301 TRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGNVSNT 360
           TRPISRTGC+A L  L GKN       ++ V+SF  N+NHDLG+  +K  ++V+ N+SN 
Sbjct: 303 TRPISRTGCEASLACLLGKNG------QYRVVSFKGNHNHDLGKMTLKRTINVDMNISNA 362

Query: 361 KKVYDADGSRSSSNTTMEFMRRKVGGVE-------NLENYTH-RKKQDLER-EATSLLQY 420
           +KV+DAD SR+S   TM  + ++V G E       +  NY    KK  +E+ E  +L+Q+
Sbjct: 363 QKVHDADSSRTSFGATMGLINKEVDGREGTGFVDRDYMNYVPIEKKARIEKGEVEALVQF 422

Query: 421 FRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARSI 445
           F  +Q+    +F+    A Q ++D+ + NIFWTDARS+
Sbjct: 423 FENKQRDNSSIFY----ALQHNEDNMVTNIFWTDARSV 448

BLAST of MC09g1251 vs. NCBI nr
Match: XP_022139322.1 (nicotinamidase 2-like isoform X3 [Momordica charantia])

HSP 1 Score: 399 bits (1025), Expect = 3.94e-136
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 5   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 64
           MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI
Sbjct: 1   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 60

Query: 65  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 124
           PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT
Sbjct: 61  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 120

Query: 125 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 184
           PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT
Sbjct: 121 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 180

Query: 185 LAYGFAYLVDRQRLEELS 202
           LAYGFAYLVDRQRLEELS
Sbjct: 181 LAYGFAYLVDRQRLEELS 198

BLAST of MC09g1251 vs. NCBI nr
Match: XP_022139321.1 (nicotinamidase 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 399 bits (1025), Expect = 6.06e-136
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 5   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 64
           MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI
Sbjct: 1   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 60

Query: 65  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 124
           PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT
Sbjct: 61  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 120

Query: 125 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 184
           PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT
Sbjct: 121 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 180

Query: 185 LAYGFAYLVDRQRLEELS 202
           LAYGFAYLVDRQRLEELS
Sbjct: 181 LAYGFAYLVDRQRLEELS 198

BLAST of MC09g1251 vs. ExPASy TrEMBL
Match: A0A5A7SVW7 (Protein FAR1-RELATED SEQUENCE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00530 PE=3 SV=1)

HSP 1 Score: 545 bits (1405), Expect = 4.12e-189
Identity = 296/462 (64.07%), Postives = 351/462 (75.97%), Query Frame = 0

Query: 1   NQKSMAAAAGSC----SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATI 60
           N KS  AAA S     SSYKKYEIRHRLP+PKIAALLVIDMQNYFSAMA+PI PN+L TI
Sbjct: 3   NPKSKPAAATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPNILTTI 62

Query: 61  RLCRAASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKN 120
           RLCRAASIPVIFTRHAH+S SDNPIL EWWNGDIILDGT AA LI DL    ADEVIRKN
Sbjct: 63  RLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADEVIRKN 122

Query: 121 TYSAFRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADED 180
           TYSAFRNT LEDCL+ KGVEEVIVTGVMTN+CCETTAREAFVRGYRVFFSTDATAT D +
Sbjct: 123 TYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLE 182

Query: 181 LHEATLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEF-DLEDNIESRINTELEIMEP 240
           LHEATLK LAYGFAYLVD +RL E+S     GQ CN+SE  DLED+ ++RI+ +LEI+EP
Sbjct: 183 LHEATLKNLAYGFAYLVDCKRLGEISAMEKIGQPCNNSEVVDLEDDRKARIDPQLEIVEP 242

Query: 241 GIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVF---KEGFRQVDKRRE 300
            IGMKF S+EE YQFYVNY  KVGF+VRK + KKKKNG++SRA F   KEGFRQV+KR+E
Sbjct: 243 VIGMKFSSVEETYQFYVNYATKVGFTVRKQYHKKKKNGIVSRASFCCSKEGFRQVNKRKE 302

Query: 301 NVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGN 360
            V +TRPISRTGC+A L  L GKN       ++ V+SF  N+NHDLG+ +MK  + V+ N
Sbjct: 303 FVHYTRPISRTGCEASLACLLGKNG------QYRVVSFKGNHNHDLGKMKMKRTMDVDTN 362

Query: 361 VSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLE-------NYTH-RKKQDLER-EATS 420
           +SN +KV+DAD SR+S   TM  + ++V G E          NY    KK  +E+ E  +
Sbjct: 363 ISNAQKVHDADSSRTSFRATMGLINKEVDGREGTGFVDKDYINYVPIEKKARIEKGEVEA 422

Query: 421 LLQYFRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARSI 445
           L+QYF ++Q+    +F+    A QL++D+ + +IFWTD RS+
Sbjct: 423 LVQYFEKKQRDNSSIFY----ALQLNEDNMVASIFWTDTRSV 454

BLAST of MC09g1251 vs. ExPASy TrEMBL
Match: A0A1S4DX27 (uncharacterized protein LOC103488764 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488764 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 2.33e-181
Identity = 288/441 (65.31%), Postives = 333/441 (75.51%), Query Frame = 0

Query: 1   NQKSMAAAAGSC----SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATI 60
           N KS  AAA S     SSYKKYEIRHRLP+PKIAALLVIDMQNYFSAMA+PI PN+L TI
Sbjct: 3   NPKSKPAAATSTDAISSSYKKYEIRHRLPNPKIAALLVIDMQNYFSAMAEPIFPNILTTI 62

Query: 61  RLCRAASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKN 120
           RLCRAASIPVIFTRHAH+S SDNPIL EWWNGDIILDGT AA LI DL    ADEVIRKN
Sbjct: 63  RLCRAASIPVIFTRHAHASASDNPILNEWWNGDIILDGTHAAGLISDLDREDADEVIRKN 122

Query: 121 TYSAFRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADED 180
           TYSAFRNT LEDCL+ KGVEEVIVTGVMTN+CCETTAREAFVRGYRVFFSTDATAT D +
Sbjct: 123 TYSAFRNTRLEDCLKEKGVEEVIVTGVMTNICCETTAREAFVRGYRVFFSTDATATVDLE 182

Query: 181 LHEATLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEF-DLEDNIESRINTELEIMEP 240
           LHEATLK LAYGFAYLVD +RL E+S     GQ CN+SE  DLED+ ++RI+ +LEI+EP
Sbjct: 183 LHEATLKNLAYGFAYLVDCKRLGEISAMEKIGQPCNNSEVVDLEDDRKARIDPQLEIVEP 242

Query: 241 GIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVF---KEGFRQVDKRRE 300
            IGMKF S+EE YQFYVNY  KVGF+VRK + KKKKNG++SRA F   KEGFRQV+KR+E
Sbjct: 243 VIGMKFSSVEETYQFYVNYATKVGFTVRKQYHKKKKNGIVSRASFCCSKEGFRQVNKRKE 302

Query: 301 NVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGN 360
            V +TRPISRTGC+A L  L GKN       ++ V+SF  N+NHDLG+ +MK  + V+ N
Sbjct: 303 FVHYTRPISRTGCEASLACLLGKNG------QYRVVSFKGNHNHDLGKMKMKRTMDVDTN 362

Query: 361 VSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLE-------NYTH-RKKQDLEREATSL 420
           +SN +KV+DAD SR+S   TM  + ++V G E          NY    KKQ+L+RE    
Sbjct: 363 ISNAQKVHDADSSRTSFRATMGLINKEVDGREGTGFVDKDYINYVPIEKKQELKREKLKH 422

Query: 421 LQYFRERQKGGILLFFFFFNA 425
           L     + +G  LLFF   N+
Sbjct: 423 LYSILRKNRGTTLLFFMHCNS 437

BLAST of MC09g1251 vs. ExPASy TrEMBL
Match: A0A0A0KLA3 (Protein FAR1-RELATED SEQUENCE OS=Cucumis sativus OX=3659 GN=Csa_5G175740 PE=3 SV=1)

HSP 1 Score: 537 bits (1383), Expect = 3.43e-181
Identity = 289/458 (63.10%), Postives = 350/458 (76.42%), Query Frame = 0

Query: 1   NQKSMAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCR 60
           N KS  +AA    SYKKYEIRHRLP+PK+AALLVIDMQNYFSAMAKPI P++L TIRLCR
Sbjct: 3   NPKSKPSAA--IPSYKKYEIRHRLPNPKVAALLVIDMQNYFSAMAKPIFPHILTTIRLCR 62

Query: 61  AASIPVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSA 120
           AASIPVIFTRHAH+S SDNPIL EWWNGDIIL+GTPAA LI DL    ADEVIRKNTYSA
Sbjct: 63  AASIPVIFTRHAHASASDNPILNEWWNGDIILEGTPAAGLISDLDREDADEVIRKNTYSA 122

Query: 121 FRNTPLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEA 180
           FRNT LEDCL+ KGVEEVIVTGVMTN+CCETTAR+AFVRGYRVFFSTDATAT D +LHEA
Sbjct: 123 FRNTRLEDCLKEKGVEEVIVTGVMTNICCETTARDAFVRGYRVFFSTDATATVDLELHEA 182

Query: 181 TLKTLAYGFAYLVDRQRLEELSDANLHGQVCNSSEF-DLEDNIESRINTELEIMEPGIGM 240
           TLK LAYGFAYLVD +RL E+S     GQ CN+SE  D+ED+ E+RI+ +LEI+EP IGM
Sbjct: 183 TLKNLAYGFAYLVDCKRLGEISAMKKTGQPCNNSEVVDVEDDREARIDPQLEIVEPVIGM 242

Query: 241 KFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVF---KEGFRQVDKRRENVLF 300
           KF S+EE Y+FYVNY  KVGF+VRK + KKKKNG++SRA F   KEGFRQV+KR+E V +
Sbjct: 243 KFISVEETYRFYVNYATKVGFTVRKQYHKKKKNGIVSRASFCCSKEGFRQVNKRKEFVHY 302

Query: 301 TRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALSVNGNVSNT 360
           TRPISRTGC+A L  L GKN       ++ V+SF  N+NHDLG+  +K  ++V+ N+SN 
Sbjct: 303 TRPISRTGCEASLACLLGKNG------QYRVVSFKGNHNHDLGKMTLKRTINVDMNISNA 362

Query: 361 KKVYDADGSRSSSNTTMEFMRRKVGGVE-------NLENYTH-RKKQDLER-EATSLLQY 420
           +KV+DAD SR+S   TM  + ++V G E       +  NY    KK  +E+ E  +L+Q+
Sbjct: 363 QKVHDADSSRTSFGATMGLINKEVDGREGTGFVDRDYMNYVPIEKKARIEKGEVEALVQF 422

Query: 421 FRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARSI 445
           F  +Q+    +F+    A Q ++D+ + NIFWTDARS+
Sbjct: 423 FENKQRDNSSIFY----ALQHNEDNMVTNIFWTDARSV 448

BLAST of MC09g1251 vs. ExPASy TrEMBL
Match: A0A6J1CDM5 (nicotinamidase 2-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010264 PE=3 SV=1)

HSP 1 Score: 399 bits (1025), Expect = 1.91e-136
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 5   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 64
           MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI
Sbjct: 1   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 60

Query: 65  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 124
           PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT
Sbjct: 61  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 120

Query: 125 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 184
           PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT
Sbjct: 121 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 180

Query: 185 LAYGFAYLVDRQRLEELS 202
           LAYGFAYLVDRQRLEELS
Sbjct: 181 LAYGFAYLVDRQRLEELS 198

BLAST of MC09g1251 vs. ExPASy TrEMBL
Match: A0A6J1CCB3 (nicotinamidase 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010264 PE=3 SV=1)

HSP 1 Score: 399 bits (1025), Expect = 2.93e-136
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 5   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 64
           MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI
Sbjct: 1   MAAAAGSCSSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASI 60

Query: 65  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 124
           PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT
Sbjct: 61  PVIFTRHAHSSPSDNPILGEWWNGDIILDGTPAADLIPDLGPVAADEVIRKNTYSAFRNT 120

Query: 125 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 184
           PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT
Sbjct: 121 PLEDCLRAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKT 180

Query: 185 LAYGFAYLVDRQRLEELS 202
           LAYGFAYLVDRQRLEELS
Sbjct: 181 LAYGFAYLVDRQRLEELS 198

BLAST of MC09g1251 vs. TAIR 10
Match: AT5G23230.1 (nicotinamidase 2 )

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-67
Identity = 125/188 (66.49%), Postives = 151/188 (80.32%), Query Frame = 0

Query: 13  SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASIPVIFTRHA 72
           SS + YE R R P+PKIAALLVIDMQN+F +MA+PIL N L TI +CR ASIPV FTRH 
Sbjct: 4   SSSRTYETRKREPNPKIAALLVIDMQNHFYSMAEPILQNALTTIDICRRASIPVFFTRHN 63

Query: 73  HSSPSDNPILGEWWNGDIILDGTPAADLIPDLG--PVAADEVIRKNTYSAFRNTPLEDCL 132
           H SP+D+ +LGEWWNGD+ILDGT  +++IP++       DE++ K+TYSAF NT L++ L
Sbjct: 64  HKSPTDHGMLGEWWNGDLILDGTTDSEIIPEINRQVTGPDEIVEKSTYSAFNNTHLQEKL 123

Query: 133 RAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLAYGFA 192
              GV+EVIV GVMTN+CCETTAREAFV+G+RVFFSTDATAT +E+LHEATL  LAYGFA
Sbjct: 124 DKIGVKEVIVIGVMTNLCCETTAREAFVKGFRVFFSTDATATVNEELHEATLMNLAYGFA 183

Query: 193 YLVDRQRL 199
           YLVD  RL
Sbjct: 184 YLVDCDRL 191

BLAST of MC09g1251 vs. TAIR 10
Match: AT5G23220.1 (nicotinamidase 3 )

HSP 1 Score: 246.5 bits (628), Expect = 4.0e-65
Identity = 124/188 (65.96%), Postives = 152/188 (80.85%), Query Frame = 0

Query: 13  SSYKKYEIRHRLPDPKIAALLVIDMQNYFSAMAKPILPNVLATIRLCRAASIPVIFTRHA 72
           SS +KYE R R P+ KIAALLVIDMQN+FS+MAKPIL NVL TI +CR AS+PV FTRH 
Sbjct: 4   SSTRKYETRKRDPNSKIAALLVIDMQNHFSSMAKPILNNVLTTIDICRRASVPVFFTRHN 63

Query: 73  HSSPSDNPILGEWWNGDIILDGTPAADLIPDL-GPVAA-DEVIRKNTYSAFRNTPLEDCL 132
           H SP+D+ +LGEW NGD+ILDGT  +++I ++ G V   DE++ KNTYSAF  T L++ L
Sbjct: 64  HKSPTDHGMLGEWCNGDVILDGTTDSEIIQEIQGQVTGPDEMVEKNTYSAFNKTRLQENL 123

Query: 133 RAKGVEEVIVTGVMTNVCCETTAREAFVRGYRVFFSTDATATADEDLHEATLKTLAYGFA 192
              GV+EVIV GVMTN+CCETTAREAF++G+RVFFSTDATAT +E+LHEATL  LA+GFA
Sbjct: 124 EKIGVKEVIVIGVMTNLCCETTAREAFIKGFRVFFSTDATATFNEELHEATLMNLAFGFA 183

Query: 193 YLVDRQRL 199
           YLVD  +L
Sbjct: 184 YLVDCDKL 191

BLAST of MC09g1251 vs. TAIR 10
Match: AT2G27110.1 (FAR1-related sequence 3 )

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-12
Identity = 59/219 (26.94%), Postives = 97/219 (44.29%), Query Frame = 0

Query: 226 INTELEIMEPGIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVFKEGFR 285
           +  E+ I EP +GM+F S +EA  FY  Y  ++GF+ +   R      V  R        
Sbjct: 40  VQDEIGIAEPCVGMEFNSEKEAKSFYDEYSRQLGFTSKLLPR--TDGSVSVREFVCSSSS 99

Query: 286 QVDKRRENVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKY 345
           +  KRR +           C A +       + +   ++W V  F + + H L  + M +
Sbjct: 100 KRSKRRLS---------ESCDAMV------RIELQGHEKWVVTKFVKEHTHGLASSNMLH 159

Query: 346 ALSVNGNVSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLENYTHRKKQDLEREATSLL 405
            L    + +N++K    +G    S      M     G  N    T+ K+  + R+A +LL
Sbjct: 160 CLRPRRHFANSEKSSYQEGVNVPSGMMYVSMDANSRGARNASMATNTKR-TIGRDAHNLL 219

Query: 406 QYFRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARS 445
           +YF+  Q         FF A QLD+D+++ N+FW D+RS
Sbjct: 220 EYFKRMQAENP----GFFYAVQLDEDNQMSNVFWADSRS 236

BLAST of MC09g1251 vs. TAIR 10
Match: AT2G27110.2 (FAR1-related sequence 3 )

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-12
Identity = 59/219 (26.94%), Postives = 97/219 (44.29%), Query Frame = 0

Query: 226 INTELEIMEPGIGMKFGSIEEAYQFYVNYGVKVGFSVRKNHRKKKKNGVISRAVFKEGFR 285
           +  E+ I EP +GM+F S +EA  FY  Y  ++GF+ +   R      V  R        
Sbjct: 40  VQDEIGIAEPCVGMEFNSEKEAKSFYDEYSRQLGFTSKLLPR--TDGSVSVREFVCSSSS 99

Query: 286 QVDKRRENVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKY 345
           +  KRR +           C A +       + +   ++W V  F + + H L  + M +
Sbjct: 100 KRSKRRLS---------ESCDAMV------RIELQGHEKWVVTKFVKEHTHGLASSNMLH 159

Query: 346 ALSVNGNVSNTKKVYDADGSRSSSNTTMEFMRRKVGGVENLENYTHRKKQDLEREATSLL 405
            L    + +N++K    +G    S      M     G  N    T+ K+  + R+A +LL
Sbjct: 160 CLRPRRHFANSEKSSYQEGVNVPSGMMYVSMDANSRGARNASMATNTKR-TIGRDAHNLL 219

Query: 406 QYFRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARS 445
           +YF+  Q         FF A QLD+D+++ N+FW D+RS
Sbjct: 220 EYFKRMQAENP----GFFYAVQLDEDNQMSNVFWADSRS 236

BLAST of MC09g1251 vs. TAIR 10
Match: AT4G15090.1 (FRS (FAR1 Related Sequences) transcription factor family )

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-07
Identity = 57/229 (24.89%), Postives = 99/229 (43.23%), Query Frame = 0

Query: 233 MEPGIGMKFGSIEEAYQFYVNYGVKVGF--SVRKNHRKKKKNGVISR--AVFKEGFRQVD 292
           +EP  G+ F + E AY FY  Y   +GF  S++ + R KK    I    A  + G     
Sbjct: 49  LEPRNGIDFDTHEAAYIFYQEYAKSMGFTTSIKNSRRSKKTKDFIDAKFACSRYGVTPES 108

Query: 293 KRRENVLFTRPISRTGCQAQLTFLHGKNVNMPAWKEWSVLSFNRNYNHDLGRTRMKYALS 352
           +   +      + +T C+A +      +V      +W +  F +++NH+L    + Y   
Sbjct: 109 ESSGSSSRRSTVKKTDCKASM------HVKRRPDGKWIIHEFVKDHNHEL-LPALAYHFR 168

Query: 353 VNGNVSNTKKVYDADGSRSSSNTTMEF---MRRKVGGVENLENYTHRKKQDL-------- 412
           +  NV   +K  + D   + S  T +    M R+ GG +N+ +                 
Sbjct: 169 IQRNVKLAEK-NNIDILHAVSERTKKMYVEMSRQSGGYKNIGSLLQTDVSSQVDKGRYLA 228

Query: 413 --EREATSLLQYFRERQKGGILLFFFFFNAFQLDQDDKIVNIFWTDARS 445
             E ++  LL+YF+  +K        FF A  L++D ++ N+FW DA+S
Sbjct: 229 LEEGDSQVLLEYFKRIKKENPK----FFYAIDLNEDQRLRNLFWADAKS 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMX72.1e-6666.49Nicotinamidase 2 OS=Arabidopsis thaliana OX=3702 GN=NIC2 PE=1 SV=1[more]
Q9FMX85.6e-6465.96Nicotinamidase 3 OS=Arabidopsis thaliana OX=3702 GN=NIC3 PE=1 SV=1[more]
P457432.7e-1834.27Isochorismatase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbB PE=1 SV=1[more]
P0ADI56.1e-1834.32Enterobactin synthase component B OS=Escherichia coli O157:H7 OX=83334 GN=entB P... [more]
P0ADI46.1e-1834.32Enterobactin synthase component B OS=Escherichia coli (strain K12) OX=83333 GN=e... [more]
Match NameE-valueIdentityDescription
KAA0034066.18.51e-18964.07nicotinamidase 2-like isoform X2 [Cucumis melo var. makuwa][more]
XP_016900280.14.81e-18165.31PREDICTED: uncharacterized protein LOC103488764 isoform X1 [Cucumis melo] >XP_01... [more]
XP_031741897.17.08e-18163.10protein FAR1-RELATED SEQUENCE 5 isoform X1 [Cucumis sativus] >KGN50450.1 hypothe... [more]
XP_022139322.13.94e-136100.00nicotinamidase 2-like isoform X3 [Momordica charantia][more]
XP_022139321.16.06e-136100.00nicotinamidase 2-like isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5A7SVW74.12e-18964.07Protein FAR1-RELATED SEQUENCE OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S4DX272.33e-18165.31uncharacterized protein LOC103488764 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KLA33.43e-18163.10Protein FAR1-RELATED SEQUENCE OS=Cucumis sativus OX=3659 GN=Csa_5G175740 PE=3 SV... [more]
A0A6J1CDM51.91e-136100.00nicotinamidase 2-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010264 ... [more]
A0A6J1CCB32.93e-136100.00nicotinamidase 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010264 ... [more]
Match NameE-valueIdentityDescription
AT5G23230.11.5e-6766.49nicotinamidase 2 [more]
AT5G23220.14.0e-6565.96nicotinamidase 3 [more]
AT2G27110.13.9e-1226.94FAR1-related sequence 3 [more]
AT2G27110.23.9e-1226.94FAR1-related sequence 3 [more]
AT4G15090.11.3e-0724.89FRS (FAR1 Related Sequences) transcription factor family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004330FAR1 DNA binding domainPFAMPF03101FAR1coord: 249..338
e-value: 9.9E-13
score: 48.6
IPR036380Isochorismatase-like superfamilyGENE3D3.40.50.850coord: 2..220
e-value: 4.4E-53
score: 182.1
IPR036380Isochorismatase-like superfamilySUPERFAMILY52499Isochorismatase-like hydrolasescoord: 17..198
IPR000868Isochorismatase-likePFAMPF00857Isochorismatasecoord: 31..196
e-value: 1.3E-44
score: 152.4
NoneNo IPR availablePANTHERPTHR43540:SF6NICOTINAMIDASE 2-RELATEDcoord: 12..200
NoneNo IPR availablePANTHERPTHR43540PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATEDcoord: 12..200
NoneNo IPR availableCDDcd00431cysteine_hydrolasescoord: 31..186
e-value: 1.54348E-56
score: 182.468

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1251.1MC09g1251.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding