MC09g1010 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1010
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionsec14 cytosolic factor-like
LocationMC09: 15798866 .. 15803318 (+)
RNA-Seq ExpressionMC09g1010
SyntenyMC09g1010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGATTTATTTTCTGTGTGTTTGCAGGCGTACGGCGACGCGACGATGATGAGATTCTTGATCGCGCGATCGATGGAGCCGGCGAAGGGAGCGAAGATGTTCGTGCAGTGGAGGAAGTGGAGGGAGGCGATGGTTCCGGAGGGATCCGTGTGCGAATCGGAGGTTGCGGAGGAGTTGGAAGCGAGGAAGATTTTCTTGCAGGGATCGTCGAAGAAGGGACTGGCCGTGGTGATTGTGAAGGCCAGTAGGCATTTTCCTTCCAAGGATCAACTCCAGTTCAAGAGTAGTTTTGTTTTCTCATCATTTCATCTTCTTTTCTTTTCTTTTCTTTTCAGTTCCAACTTCCAATGCGTGAAAATTTTTCCGCTGTTTCGTTTTCTGTTTTCCAAATTAAAATTCTCACTTCTTTTCTTTTCTTCTCTCTCTTTCTTTCCTTTTTCCATTTTAACTGTTGGAGTGGTTTCCATTTTAATGATTCAATTCCAACCTACAATTGCTAAACCAAAATAATGAAAATTTAAGATGTAAAATATATTTCTGGTCATTTTGAGTCATATCGGTACAAAGAAGATTGAAAAACTTTTAGAAATTGAAGGGTTAAAACAATGCGTAAGTTGCAAATATGACAAAAAAAAAAAAAATTAGTAAAATTAGCAAAGTAGCAGATCCAATTTTAAAATGACAACTTTATCTAAAATAGTATACATCTTTAATATATCTGATTACTAATATATATATGTGATATACATATGATATATTGCAATATGTATATGATATACATCTAATATATTATTGATATATGTGTCTGTGCAATATGTATGTGATATATATCTAATGTACTATTTATATGAGTGTTTGATATACTACTTATATATAATTATTGATTTATTGATTTTAATATCTCATATACTATTAATATAAGTGAATATTTTTACTATATTTAAAAATTTTAAAACTAGATAATATATTTTGAAATACATAAAATTTGCCGAGCATTTTATATAATTTAACCTACCTTTTAAAAATAATATTATTTAAGAAAGTATGAGAGTGGTTAATTAATAGTCACATATTTTATAGTCGTCTATAAATAAATTTCGTTTATTTTTCATTTTTGATAGGTAGGATTTTATAGTTTGTTCATCTGATTTAATCTCCTCTCTCTCTCTTCTATGCAGAGTTTGTGGTTCATCTCTTGGATAAGGTCATTGCAAGGTGACCTACATAATCTCAATCATAATTAACAAAATTAGAATTTTAAATTATTATCCTGTATCCCGTTATTGATTCTTATTATGAATAATCTCAATCTTTTATATATATATATATATATATATATATATATATAANATTATATAGTTTTTTAAACATGTCAGATAAGATGCTACCTTCAATTTCAATTCCTTACCCTTTTTCTATAAATCTGAAAATATATTGCTATAAATGAAGACCTTATTTTTATTTCTATGCTTTAATTTTCTCTGATAATTTCATGGGTTAATTGAAAACTACTATTTTACAACCATTTTGTTTGTCTTTTATCAATATTTATTAAAAAAAATACTTTTATTTTTAGTTTTTTTATTTGTTTTTTTTTTAAATAAATTTTGAATCTAAGTTATAAAAACAGGATCATATTTTTTTAACACTACTCTTTTCTTTTACTTTTTTATATTTTGCTATAATTTTTAAATTGTATTAAACTATATATTACACATAAAATAAATTAGCTTTGAAAAAAGAACATGTGGGACAAGACAAAATGGTTTCCAATAAAATTTTCTGTTTTTTTTTTAATTTCTAAATAATGTTATCGAAATGTTTTTCAATAGTAACCTTAAAAATAATAATAACAATAATAGTAACCGCTATTGAAAATTAAGTAAAAAATTCAAAAACATTTATTTTTAAAAATGGAAATGAAATTTTTAAGAGGAGAAAAATAAAAGTAAAGATTTTTATATATGGACAGCGGTTGCAAAGGAAGAGAAATTGGGAATGAAAAGTGGATTGCCATTGTTGATCTGCATCAAATCTCATATAAGAATGTTGATCCACGTGGCCTCATCACAGGGTTTCAATTTTTACAGGTATCCGCTAATTTTTATTTTTTATTTTCCAAAATATTACATTTATACGTTTGGGTCAATTAAGACCTGTCTGTTGACCATTCGATTCTGTTTCTTTTGTTTTCTGATTTACTGAATATTTTTTTCAAATTGTTTTCGGATGATCAAAATAATGAAAATTCTGAAATAATATTTTTATGTTTATGATTTTGAATTTGATACTAAAAAATGTAAAATTATATTAAAAACAATACAAAAACGTTGGTTAAGATATCATTTTATTTTATAAACTCATTTATTCGTTTTAAAATTAAAACATGAAAATATAATATATTATAAATTATATAATATTATAAAACAATAATTATCCAAAATTTATAACATAAATGTTAATAAATTAATAAATATTATTTTATATTAAACTAAATATTTACTTTGTAATTTCTACTTGTTTACAAATATATTGTTAAACAAACGCTGGATAATTTTTTAAATTGAGATTCAATTTATCTAAAAATACTAAGATAAAGAATACAATAATGTTTTTCTATTAAATCTCTTGTTTTTAGATTTTTTATTTTTTGATCATCAATCAAACGCACTTTTAAAAACTTAGGGATTAAATAGAACCATCCTCCAAATCTCAAGACCAAAAAAATAATTTATCATATAATTTTCTTTATATAATTTTAAAATTTTTACTAACATACCTCTATTTCTCTACTTTATACTTGTAACATAATATTCTTTAAAAAAAAAACAAAGAAAGAAAGAAAGAAAATAAACTATAAATTTTAAAAGTTCTTTCAATTTCAGCTGTATTTTTTAGTTTCGATGTTTATATTTAAGGAAAAACCAACTTTTTACCTAAACGTTTGATTTCACTCCACTAAATCTTAGACTTAGATAAGTGTGGTAAATTTTTTTCCTCAATAAGTATTTATTCTCCGAAAAATAATTTTTTTTTTCCTTTTTCAAGTTTTACCAAGGTAAGAATTCGATAGGATATGTATGTAATTTTCTTTTTTAGGGTGAGAGTTATATTCAAATTAACATTAATTTTACATTTAATAGTTGTTAACATTTTATATGTAATATATTTTAAATATGTTGCAGAACTATTATCCGGAGCGTTTAGGGAAGTGCTTCATTTTAAACATGCCATGGTTTTTTGTTAGCATTTGGAGAATGGTTTCTCGGTTCGTCGATAGTGCAACGTTGAAAAACGTAAGCCGTTCCTATTATTTCATCTCATTTACTTTTCTTTCAATTAACCGTTCTATTTAGTTAAATTACACAATGTTGTTATGCTTATTTTGTTTTAATTTGGTCTCATTTAAAAATCTTGATTTAGTCTATAATTTGAACTTCATTTTATTGTGTGACTCTCCCTTTCATTTACCATTTTAAAGTTTAATAAAAGTTAATATATTTATTATCATCTTTGAATATTATTTTAGAAAATAGGAATAAAATTTTTAAAAAATTCTATTTATTTTCAAATTTAAATATTATTTTGGTTCCATTATTTTGACTTTGGTTAATTTTCGTTCGAGTACTTTTAGAACTCCTATTTTAGTTCACCTACTTTCAATTTTTTAATTTTATTTTTTTGTCTATTTTAATCCGTATACTTTCAAAACATCTTATTTTGATCTTTAGAACTTTTGAAACATTGTCATTTTAGTCTTTTCATTTTATTTTTTATCTCATTTTTTCAACACAAATTTCAGTATGATATCACTAGCCATAAATTTTTATTAAAGAATTTTTTAAGTGTATAGTATTTGTGTATAAACTTTGCAAACAAATACCAAAATTTCAGCTTTAGTTTCTGTAATCTCAATTTTTATTCATTTTAGTTATCGTACTCTCAACTTTCATATATTCTAATTTAATTATTATAATTCTAATACATCTGTTTATGATTGAAACTGAAAAGTATAAAGACTAAAATAGTATTTAAATCTACTTTTCCAATTCAATTATATATTTAGTGTATGTATGCATGATTTATATGCGCATAAACACTGATTTTCAGATTCTAATTGTGAGCAATGAAGAAGAAAGAAGAATTATGATAGAGGAAGTTGGAGAAGTATTGCCTGTCGAGTATGGTGGAAAGGCAAAGCTTATTGCTCTTCAAGATGCTATCATAATTCCTCACTTGGATGCTTAATAATTTAATGTTAGGGAAATTAAATTAGCTCCTCTCTCTCTCTGTCTCTCTCATGGATGTTTTTATGTTGTAAAATTTGTTTCTATCTCTTCAAAATGGTCATTGTGAATTGCAACCTTCAATAATCAAACTTGATCACTGACCCAACTCCATAGCAAAAATAGGTTCGATGTTTGAATAATATTTAGGTGTTTATCGTTCGGTA

mRNA sequence

GTGGATTTATTTTCTGTGTGTTTGCAGGCGTACGGCGACGCGACGATGATGAGATTCTTGATCGCGCGATCGATGGAGCCGGCGAAGGGAGCGAAGATGTTCGTGCAGTGGAGGAAGTGGAGGGAGGCGATGGTTCCGGAGGGATCCGTGTGCGAATCGGAGGTTGCGGAGGAGTTGGAAGCGAGGAAGATTTTCTTGCAGGGATCGTCGAAGAAGGGACTGGCCGTGGTGATTGTGAAGGCCAGTAGGCATTTTCCTTCCAAGGATCAACTCCAGTTCAAGAAGTTTGTGGTTCATCTCTTGGATAAGGTCATTGCAAGCGGTTGCAAAGGAAGAGAAATTGGGAATGAAAAGTGGATTGCCATTGTTGATCTGCATCAAATCTCATATAAGAATGTTGATCCACGTGGCCTCATCACAGGGTTTCAATTTTTACAGAACTATTATCCGGAGCGTTTAGGGAAGTGCTTCATTTTAAACATGCCATGGTTTTTTGTTAGCATTTGGAGAATGGTTTCTCGGTTCGTCGATAGTGCAACGTTGAAAAACATTCTAATTGTGAGCAATGAAGAAGAAAGAAGAATTATGATAGAGGAAGTTGGAGAAGTATTGCCTGTCGAGTATGGTGGAAAGGCAAAGCTTATTGCTCTTCAAGATGCTATCATAATTCCTCACTTGGATGCTTAATAATTTAATGTTAGGGAAATTAAATTAGCTCCTCTCTCTCTCTGTCTCTCTCATGGATGTTTTTATGTTGTAAAATTTGTTTCTATCTCTTCAAAATGGTCATTGTGAATTGCAACCTTCAATAATCAAACTTGATCACTGACCCAACTCCATAGCAAAAATAGGTTCGATGTTTGAATAATATTTAGGTGTTTATCGTTCGGTA

Coding sequence (CDS)

GTGGATTTATTTTCTGTGTGTTTGCAGGCGTACGGCGACGCGACGATGATGAGATTCTTGATCGCGCGATCGATGGAGCCGGCGAAGGGAGCGAAGATGTTCGTGCAGTGGAGGAAGTGGAGGGAGGCGATGGTTCCGGAGGGATCCGTGTGCGAATCGGAGGTTGCGGAGGAGTTGGAAGCGAGGAAGATTTTCTTGCAGGGATCGTCGAAGAAGGGACTGGCCGTGGTGATTGTGAAGGCCAGTAGGCATTTTCCTTCCAAGGATCAACTCCAGTTCAAGAAGTTTGTGGTTCATCTCTTGGATAAGGTCATTGCAAGCGGTTGCAAAGGAAGAGAAATTGGGAATGAAAAGTGGATTGCCATTGTTGATCTGCATCAAATCTCATATAAGAATGTTGATCCACGTGGCCTCATCACAGGGTTTCAATTTTTACAGAACTATTATCCGGAGCGTTTAGGGAAGTGCTTCATTTTAAACATGCCATGGTTTTTTGTTAGCATTTGGAGAATGGTTTCTCGGTTCGTCGATAGTGCAACGTTGAAAAACATTCTAATTGTGAGCAATGAAGAAGAAAGAAGAATTATGATAGAGGAAGTTGGAGAAGTATTGCCTGTCGAGTATGGTGGAAAGGCAAAGCTTATTGCTCTTCAAGATGCTATCATAATTCCTCACTTGGATGCTTAA

Protein sequence

VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA
Homology
BLAST of MC09g1010 vs. ExPASy Swiss-Prot
Match: P45816 (SEC14 cytosolic factor OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 3.7e-11
Identity = 54/223 (24.22%), Postives = 106/223 (47.53%), Query Frame = 0

Query: 13  DATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQ---GS 72
           DAT++RFL AR  +     +M+    KWR+       + +    E+ E  K++ Q    +
Sbjct: 54  DATLLRFLRARKFDVPLAQEMWENCEKWRKEFGTNTILEDFWYKEKKEVAKLYPQYYHKT 113

Query: 73  SKKGLAVVIVKASR-------HFPSKDQL------QFKKFVVHLLDKVIASGCKGREIGN 132
            K G  V +    +          +++++      +++ FV H L       C  R +G+
Sbjct: 114 DKDGRPVYVENVGKVNIHEMYKITTQERMLRNLVWEYESFVRHRL-----PAC-SRVVGH 173

Query: 133 --EKWIAIVDLHQISYKNVDPRGLITGF-----QFLQNYYPERLGKCFILNMPWFFVSIW 192
             E    I+DL  +S  +      + GF        QNYYPER+GK +++N P+ F +++
Sbjct: 174 LIETSCTILDLKGVSLSSASQ---VYGFLKDASNIGQNYYPERMGKFYLINAPFGFSTVF 233

Query: 193 RMVSRFVDSATLKNILIVSNEEERRIMIEEVGEVLPVEYGGKA 213
            ++ RF+D  T+  I +  +  + +++ +     LP+++GG++
Sbjct: 234 SVIKRFLDPVTVSKIHVYGSNYKEKLLAQVPAYNLPIKFGGQS 267

BLAST of MC09g1010 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 55/224 (24.55%), Postives = 98/224 (43.75%), Query Frame = 0

Query: 13  DATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQGSSKK 72
           DAT++RFL AR     +  +MF++  KWR+    +  +      E+    K + Q   K 
Sbjct: 50  DATLLRFLRARKFNLQQSLEMFIKCEKWRKEFGVDDLIKNFHYDEKEAVSKYYPQFYHKT 109

Query: 73  GL--AVVIVKASRHFPSK-------------------DQLQFKKFVV--HLLDKVIASGC 132
            +    V V+   +   K                   + L  K+F         +I + C
Sbjct: 110 DIDGRPVYVEQLGNIDLKKLYQITTPERMMQNLVYEYEMLALKRFPACSRKAGGLIETSC 169

Query: 133 KGREIGNEKWIAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIW 192
               I + K + I  +H + Y  +     I+     Q+YYPER+GK +++N PW F S +
Sbjct: 170 ---TIMDLKGVGITSIHSV-YSYIRQASSIS-----QDYYPERMGKFYVINAPWGFSSAF 229

Query: 193 RMVSRFVDSATLKNILIVSNEEERRIMIEEVGEVLPVEYGGKAK 214
            ++  F+D AT+K I I+ +  +  ++ +   + LP + GG  +
Sbjct: 230 NLIKGFLDEATVKKIHILGSNYKSALLEQIPADNLPAKLGGNCQ 264

BLAST of MC09g1010 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 58/222 (26.13%), Postives = 107/222 (48.20%), Query Frame = 0

Query: 8   LQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQ 67
           ++   D+T++RFL AR  +     +MF    KWR+    +  + +    E+    K + Q
Sbjct: 50  IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYGTDTILQDFHYDEKPLIAKFYPQ 109

Query: 68  ---GSSKKGLAVVIVK---ASRHFPSK--DQLQFKKFVVHLLDKVI---ASGCKGREIGN 127
               + K G  V   +    + H  +K   + +  K +V   + V+      C  R  G+
Sbjct: 110 YYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNLVWEYESVVQYRLPAC-SRAAGH 169

Query: 128 --EKWIAIVDLHQISYKNV-DPRGLITGFQFL-QNYYPERLGKCFILNMPWFFVSIWRMV 187
             E    I+DL  IS  +       +    ++ QNYYPER+GK +I+N P+ F + +R+ 
Sbjct: 170 LVETSCTIMDLKGISISSAYSVMSYVREASYISQNYYPERMGKFYIINAPFGFSTAFRLF 229

Query: 188 SRFVDSATLKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKL 215
             F+D  T+  I I+ +  ++ ++ +   E LPV++GGK+++
Sbjct: 230 KPFLDPVTVSKIFILGSSYQKELLKQIPAENLPVKFGGKSEV 270

BLAST of MC09g1010 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 59/223 (26.46%), Postives = 106/223 (47.53%), Query Frame = 0

Query: 13  DATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSV------CESEVAEELEARKIFL 72
           D+T++RFL AR  +     +MFV+  +WRE       +       E+E  E ++  K++ 
Sbjct: 51  DSTLLRFLRARKFDINASVEMFVETERWREEYGANTIIEDYENNKEAEDKERIKLAKMYP 110

Query: 73  Q---GSSKKG-------LAVVIVKASRHFPSKDQL---QFKKFVVHLLDKVIASGCKGRE 132
           Q      K G       L  + +K      ++ Q+     K++ +    +V A   +   
Sbjct: 111 QYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLRNLVKEYELFATYRVPACSRRAGY 170

Query: 133 IGNEKWIAIVDLHQISYKNVDP--RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRM 192
           +  E    ++DL  IS  N       +       QNYYPER+GK +I++ P+ F ++++M
Sbjct: 171 L-IETSCTVLDLKGISLSNAYHVLSYIKDVADISQNYYPERMGKFYIIHSPFGFSTMFKM 230

Query: 193 VSRFVDSATLKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKL 215
           V  F+D  T+  I I+ +  ++ ++ +   E LPV+YGG + L
Sbjct: 231 VKPFLDPVTVSKIFILGSSYKKELLKQIPIENLPVKYGGTSVL 272

BLAST of MC09g1010 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 44/183 (24.04%), Postives = 90/183 (49.18%), Query Frame = 0

Query: 13  DATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQGSSKK 72
           D   +R+L AR+   +K  KM     +WR+   P+      ++ E   A  +++    KK
Sbjct: 73  DMCFLRYLRARNYIVSKSEKMLRDTLEWRKKFRPQDIQLGGDIREIGSAGCVYVNKRDKK 132

Query: 73  GLAVVIV----KASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQI 132
           G  ++         ++ PS  +L+FK  +V+ L++  +   + +  G E++  IVD    
Sbjct: 133 GRPIIFAVPRNDTLKNVPS--ELKFKN-LVYWLEQGFSRMDEPK--GIEQFCFIVDYKDF 192

Query: 133 SYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVS 192
              N+D +  +    FL ++ PER+G+   L+ P  F   W+++S F++  TL  +  ++
Sbjct: 193 GSGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKIISPFLNEVTLSKVRFIN 250

BLAST of MC09g1010 vs. NCBI nr
Match: XP_022146735.1 (sec14 cytosolic factor-like [Momordica charantia])

HSP 1 Score: 438 bits (1126), Expect = 2.17e-154
Identity = 220/228 (96.49%), Postives = 222/228 (97.37%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+      +AYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE
Sbjct: 16  VEKLGFSTEAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI
Sbjct: 76  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT
Sbjct: 136 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA
Sbjct: 196 LKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA 243

BLAST of MC09g1010 vs. NCBI nr
Match: KAG6599921.1 (hypothetical protein SDJN03_05154, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 380 bits (976), Expect = 1.57e-131
Identity = 194/229 (84.72%), Postives = 207/229 (90.39%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+   +  Q Y DATMMRFLIARSMEP+KGAKMFVQWRKWR+A+VP+G +CESEV EELE
Sbjct: 16  VEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFICESEVKEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           A+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVIASG KG+EIGNEKWI
Sbjct: 76  AKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD AT
Sbjct: 136 AIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIVSNEEERR MIEEVGE VLP EYGGKAK IALQDA++ PHLDA
Sbjct: 196 LKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-PHLDA 243

BLAST of MC09g1010 vs. NCBI nr
Match: XP_022941638.1 (SEC14 cytosolic factor-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 376 bits (966), Expect = 5.22e-130
Identity = 193/229 (84.28%), Postives = 206/229 (89.96%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+   +  Q Y DATMMRFLIARSMEP+KGAKMFVQWRKWR+A+VP+G + ESEV EELE
Sbjct: 16  VEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFISESEVKEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           A+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVIASG KG+EIGNEKWI
Sbjct: 76  AKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD AT
Sbjct: 136 AIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIVSNEEERR MIEEVGE VLP EYGGKAK IALQDA++ PHLDA
Sbjct: 196 LKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-PHLDA 243

BLAST of MC09g1010 vs. NCBI nr
Match: XP_023537304.1 (SEC14 cytosolic factor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 376 bits (966), Expect = 5.22e-130
Identity = 192/229 (83.84%), Postives = 205/229 (89.52%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+   +  Q Y DATMMRFLIARSMEP+KGAKMFVQWRKWR+A+VP+G +CESEV EELE
Sbjct: 16  VEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFICESEVKEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           A+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVIASG KG+EIGNEKWI
Sbjct: 76  AKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD  T
Sbjct: 136 AIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPTT 195

Query: 181 LKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNI IVSNEEERR MIEEVGE VLP EYGGKAK IALQDA++ PHLDA
Sbjct: 196 LKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-PHLDA 243

BLAST of MC09g1010 vs. NCBI nr
Match: XP_022996420.1 (SEC14 cytosolic factor-like [Cucurbita maxima])

HSP 1 Score: 371 bits (953), Expect = 4.99e-128
Identity = 190/229 (82.97%), Postives = 204/229 (89.08%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+   +  Q Y DATMMRFLIARSMEP+KGAKMFVQWR WR+AMVP+G +CESEV EELE
Sbjct: 16  VEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMVPKGFICESEVKEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           A+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVIASG +G+EIGNEKWI
Sbjct: 76  AKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSEGKEIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD AT
Sbjct: 136 AIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIV N+EERR MIEEVGE VLP EYGGKAK IALQDA++ P LDA
Sbjct: 196 LKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-PRLDA 243

BLAST of MC09g1010 vs. ExPASy TrEMBL
Match: A0A6J1CY34 (sec14 cytosolic factor-like OS=Momordica charantia OX=3673 GN=LOC111015872 PE=4 SV=1)

HSP 1 Score: 438 bits (1126), Expect = 1.05e-154
Identity = 220/228 (96.49%), Postives = 222/228 (97.37%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+      +AYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE
Sbjct: 16  VEKLGFSTEAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI
Sbjct: 76  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT
Sbjct: 136 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA
Sbjct: 196 LKNILIVSNEEERRIMIEEVGEVLPVEYGGKAKLIALQDAIIIPHLDA 243

BLAST of MC09g1010 vs. ExPASy TrEMBL
Match: A0A6J1FSN7 (SEC14 cytosolic factor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446938 PE=4 SV=1)

HSP 1 Score: 376 bits (966), Expect = 2.53e-130
Identity = 193/229 (84.28%), Postives = 206/229 (89.96%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+   +  Q Y DATMMRFLIARSMEP+KGAKMFVQWRKWR+A+VP+G + ESEV EELE
Sbjct: 16  VEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFISESEVKEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           A+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVIASG KG+EIGNEKWI
Sbjct: 76  AKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD AT
Sbjct: 136 AIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIVSNEEERR MIEEVGE VLP EYGGKAK IALQDA++ PHLDA
Sbjct: 196 LKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-PHLDA 243

BLAST of MC09g1010 vs. ExPASy TrEMBL
Match: A0A6J1K6Q5 (SEC14 cytosolic factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491647 PE=4 SV=1)

HSP 1 Score: 371 bits (953), Expect = 2.41e-128
Identity = 190/229 (82.97%), Postives = 204/229 (89.08%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELE 60
           V+   +  Q Y DATMMRFLIARSMEP+KGAKMFVQWR WR+AMVP+G +CESEV EELE
Sbjct: 16  VEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMVPKGFICESEVKEELE 75

Query: 61  ARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWI 120
           A+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVIASG +G+EIGNEKWI
Sbjct: 76  AKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSEGKEIGNEKWI 135

Query: 121 AIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSAT 180
           AIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD AT
Sbjct: 136 AIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPAT 195

Query: 181 LKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHLDA 228
           LKNILIV N+EERR MIEEVGE VLP EYGGKAK IALQDA++ P LDA
Sbjct: 196 LKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-PRLDA 243

BLAST of MC09g1010 vs. ExPASy TrEMBL
Match: A0A1S3BT62 (sec14 cytosolic factor-like OS=Cucumis melo OX=3656 GN=LOC103493455 PE=4 SV=1)

HSP 1 Score: 358 bits (920), Expect = 2.56e-123
Identity = 180/219 (82.19%), Postives = 198/219 (90.41%), Query Frame = 0

Query: 9   QAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQG 68
           Q Y DATMMRFLIARSME +K AKMFVQW+KWR+A VP+G + ESEV +EL+A+KIFLQG
Sbjct: 24  QDYSDATMMRFLIARSMEVSKAAKMFVQWKKWRDATVPKGYIAESEVEDELKAKKIFLQG 83

Query: 69  SSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQI 128
            S K L V+IV A+RHF SKDQLQFKKFVVH+LDKVIASGCKG+EIGNEKWIAIVDL QI
Sbjct: 84  MSIKQLPVMIVIANRHFHSKDQLQFKKFVVHILDKVIASGCKGKEIGNEKWIAIVDLQQI 143

Query: 129 SYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVS 188
           SYKNVDPRGLIT FQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVD+ATLKNILIVS
Sbjct: 144 SYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDTATLKNILIVS 203

Query: 189 NEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHL 226
           NEEE+R MIEEVGE VLP+EYGGKAK  ALQD +++PHL
Sbjct: 204 NEEEKRNMIEEVGEDVLPIEYGGKAKFFALQD-VVVPHL 241

BLAST of MC09g1010 vs. ExPASy TrEMBL
Match: A0A0A0KL83 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G021320 PE=4 SV=1)

HSP 1 Score: 357 bits (917), Expect = 7.33e-123
Identity = 178/219 (81.28%), Postives = 198/219 (90.41%), Query Frame = 0

Query: 9   QAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQG 68
           Q Y DATMMRFLIARSME  K AKMFVQW+KWR+A VP+G + ESEV +EL+A+KIFLQG
Sbjct: 24  QDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATVPKGLIAESEVEDELKAKKIFLQG 83

Query: 69  SSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQI 128
            S K L V+IV A+RHF SKDQ+QFKKF+VHLLDKVIASGCKG+EIGNEKWIAIVDL QI
Sbjct: 84  ISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVIASGCKGKEIGNEKWIAIVDLQQI 143

Query: 129 SYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVS 188
           SYKNVDPRGLIT FQFLQNYYPERLGKCFILNMPWFFVSIWRM+SRFVD+ATLKNILIVS
Sbjct: 144 SYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFFVSIWRMISRFVDTATLKNILIVS 203

Query: 189 NEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIPHL 226
           +EEE+RIMIEEVGE VLP+EYGGKAK I LQD +++PHL
Sbjct: 204 SEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQD-VVLPHL 241

BLAST of MC09g1010 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 261.5 bits (667), Expect = 6.1e-70
Identity = 128/224 (57.14%), Postives = 169/224 (75.45%), Query Frame = 0

Query: 1   VDLFSVCLQAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV-PEGSVCESEVAEEL 60
           V+  S   + Y   T+MRFL+ARSM+P K AKMFV W+KWR +MV P G + ESEV +EL
Sbjct: 16  VEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDEL 75

Query: 61  EARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKW 120
           E RK+ LQG +K G  +V+V  S+HF SKD   FKKFVV+ LDK IASG  G+E+G EK 
Sbjct: 76  EFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKL 135

Query: 121 IAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSA 180
           +A++DL  I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++ A
Sbjct: 136 VAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKA 195

Query: 181 TLKNILIVSNEEERRIMIEEVG-EVLPVEYGGKAKLIALQDAII 223
           T + I+IV++ EE+R   EE+G + LP EYGG+AKL A+QD ++
Sbjct: 196 TQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLL 239

BLAST of MC09g1010 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 260.0 bits (663), Expect = 1.8e-69
Identity = 126/216 (58.33%), Postives = 166/216 (76.85%), Query Frame = 0

Query: 9   QAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV-PEGSVCESEVAEELEARKIFLQ 68
           + Y   T+MRFL+ARSM+P K AKMFV W+KWR +MV P G + ESEV +ELE RK+ LQ
Sbjct: 11  KGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQ 70

Query: 69  GSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQ 128
           G +K G  +V+V  S+HF SKD   FKKFVV+ LDK IASG  G+E+G EK +A++DL  
Sbjct: 71  GPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLAN 130

Query: 129 ISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIV 188
           I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++ AT + I+IV
Sbjct: 131 ITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIV 190

Query: 189 SNEEERRIMIEEVG-EVLPVEYGGKAKLIALQDAII 223
           ++ EE+R   EE+G + LP EYGG+AKL A+QD ++
Sbjct: 191 TDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLL 226

BLAST of MC09g1010 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 260.0 bits (663), Expect = 1.8e-69
Identity = 126/216 (58.33%), Postives = 166/216 (76.85%), Query Frame = 0

Query: 9   QAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV-PEGSVCESEVAEELEARKIFLQ 68
           + Y   T+MRFL+ARSM+P K AKMFV W+KWR +MV P G + ESEV +ELE RK+ LQ
Sbjct: 11  KGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQ 70

Query: 69  GSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQ 128
           G +K G  +V+V  S+HF SKD   FKKFVV+ LDK IASG  G+E+G EK +A++DL  
Sbjct: 71  GPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLAN 130

Query: 129 ISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIV 188
           I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++ AT + I+IV
Sbjct: 131 ITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIV 190

Query: 189 SNEEERRIMIEEVG-EVLPVEYGGKAKLIALQDAII 223
           ++ EE+R   EE+G + LP EYGG+AKL A+QD ++
Sbjct: 191 TDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLL 226

BLAST of MC09g1010 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-36
Identity = 77/209 (36.84%), Postives = 127/209 (60.77%), Query Frame = 0

Query: 13  DATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQGSSKK 72
           D  + RFL AR ++  K + MF+ +  W+ +M+P+G + E+E+A +L   K+ +QG  K 
Sbjct: 50  DLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIANDLSHNKMCMQGHDKM 109

Query: 73  GLAVVIVKASRHFPSK-DQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQISYK 132
           G  + +   +RH PSK +  +FK+FVV+ L+K+    C     G EK++AI DL    Y 
Sbjct: 110 GRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKI----CARMPRGQEKFVAIGDLQGWGYS 169

Query: 133 NVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEE 192
           N D RG +     LQ+ YPERLGK +I++ P+ F++ W+++  F+D+ T K I+ V N++
Sbjct: 170 NCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPFIDANTKKKIVFVENKK 229

Query: 193 ERRIMIEEVGE-VLPVEYGGKAKLIALQD 220
               ++E++ E  LP  YGGK  L+ +Q+
Sbjct: 230 LTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of MC09g1010 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.9e-13
Identity = 63/207 (30.43%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 13  DATMMRFLIARSMEPAKGAKMFVQWRKWREAMVPEGSVCESEVAEELEARKIFLQGSSKK 72
           DA++ RFL AR+ +  K  KM  +  KWR    P+  +  ++VA E E  K        +
Sbjct: 41  DASLRRFLDARNWDVEKAKKMIQETLKWRSTYKPQ-EIRWNQVAHEGETGKASRASFHDR 100

Query: 73  GLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVIASGCKGREIGNEKWIAIVDLHQISYKN 132
              VV++       S  Q    + +V+LL+  I +  KG++     W  ++D    S   
Sbjct: 101 QGRVVLIMRPAMQNSTSQEGNIRHLVYLLENAIINLPKGQK--QMSW--LIDFTGWSMA- 160

Query: 133 VDPRGLITG--FQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIV--S 192
           V+P    T      LQNYYPERLG  F+ N P  F +++R    F+D  T + +  V   
Sbjct: 161 VNPPMKTTREIIHILQNYYPERLGIAFLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPK 220

Query: 193 NEEERRIMIEEVG-EVLPVEYGGKAKL 215
           ++    +M      E LP E+GG+A L
Sbjct: 221 DKASDELMTTHFDVENLPKEFGGEATL 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P458163.7e-1124.22SEC14 cytosolic factor OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28459... [more]
Q101371.1e-1024.55Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX... [more]
P242801.4e-1026.13SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ... [more]
P333241.8e-1026.46CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ... [more]
Q55CU81.6e-0924.04Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
Match NameE-valueIdentityDescription
XP_022146735.12.17e-15496.49sec14 cytosolic factor-like [Momordica charantia][more]
KAG6599921.11.57e-13184.72hypothetical protein SDJN03_05154, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941638.15.22e-13084.28SEC14 cytosolic factor-like isoform X1 [Cucurbita moschata][more]
XP_023537304.15.22e-13083.84SEC14 cytosolic factor-like [Cucurbita pepo subsp. pepo][more]
XP_022996420.14.99e-12882.97SEC14 cytosolic factor-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CY341.05e-15496.49sec14 cytosolic factor-like OS=Momordica charantia OX=3673 GN=LOC111015872 PE=4 ... [more]
A0A6J1FSN72.53e-13084.28SEC14 cytosolic factor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1K6Q52.41e-12882.97SEC14 cytosolic factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491647 PE=4 SV=... [more]
A0A1S3BT622.56e-12382.19sec14 cytosolic factor-like OS=Cucumis melo OX=3656 GN=LOC103493455 PE=4 SV=1[more]
A0A0A0KL837.33e-12381.28CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G021320 P... [more]
Match NameE-valueIdentityDescription
AT1G14820.36.1e-7057.14Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.21.8e-6958.33Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.11.8e-6958.33Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G01630.11.5e-3636.84Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.16.9e-1330.43Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 58..213
e-value: 2.4E-28
score: 110.1
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 57..211
e-value: 8.6E-27
score: 93.8
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 52..216
score: 16.46241
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 65..211
e-value: 4.74497E-25
score: 94.3742
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 5..219
e-value: 4.8E-39
score: 136.2
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 53..213
NoneNo IPR availablePANTHERPTHR46277:SF7SEC14 CYTOSOLIC FACTOR-LIKEcoord: 4..222
NoneNo IPR availablePANTHERPTHR46277OS03G0850700 PROTEINcoord: 4..222
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 8..48

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1010.1MC09g1010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane