MC09g0999 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0999
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionUbiquitin-conjugating enzyme E2 E2
LocationMC09: 15514753 .. 15514932 (+)
RNA-Seq ExpressionMC09g0999
SyntenyMC09g0999
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGAGGTTTATTCAAACTCTCCATATATTTGCCTCCCGAGTATCCAATGAAGCCACCAAAAGTTACCTTCAAAACTAAAATTTACCATCCTAACATCAATAGTAAAGGTAATATTTGCCTTGACATTCTCAAGGGAAATTGGAGTCCTGCCTTTACACTTTCAAGTGTGTTGGTTGCC

mRNA sequence

GAGAGAGGTTTATTCAAACTCTCCATATATTTGCCTCCCGAGTATCCAATGAAGCCACCAAAAGTTACCTTCAAAACTAAAATTTACCATCCTAACATCAATAGTAAAGGTAATATTTGCCTTGACATTCTCAAGGGAAATTGGAGTCCTGCCTTTACACTTTCAAGTGTGTTGGTTGCC

Coding sequence (CDS)

GAGAGAGGTTTATTCAAACTCTCCATATATTTGCCTCCCGAGTATCCAATGAAGCCACCAAAAGTTACCTTCAAAACTAAAATTTACCATCCTAACATCAATAGTAAAGGTAATATTTGCCTTGACATTCTCAAGGGAAATTGGAGTCCTGCCTTTACACTTTCAAGTGTGTTGGTTGCC

Protein sequence

ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA
Homology
[Close]
BLAST of MC09g0999 vs. ExPASy Swiss-Prot
Match: P35134 (Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis thaliana OX=3702 GN=UBC11 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.8e-19
Identity = 40/58 (68.97%), Postives = 50/58 (86.21%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F +SI+ PP+YP KPPKV+FKTK+YHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. ExPASy Swiss-Prot
Match: P15732 (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UBC5 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 8.2e-19
Identity = 42/58 (72.41%), Postives = 47/58 (81.03%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F LSI+ P +YP KPPKV F TKIYHPNINS GNICLDILK  WSPA TLS VL++
Sbjct: 49  GVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLS 106

[Close]
BLAST of MC09g0999 vs. ExPASy Swiss-Prot
Match: Q9SLE4 (Ubiquitin-conjugating enzyme E2 29 OS=Arabidopsis thaliana OX=3702 GN=UBC29 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.1e-18
Identity = 38/58 (65.52%), Postives = 49/58 (84.48%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F ++I+ PP+YP KPPKV F+TK++HPNINS GNICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVNIHFPPDYPFKPPKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. ExPASy Swiss-Prot
Match: P15731 (Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UBC4 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-18
Identity = 40/58 (68.97%), Postives = 48/58 (82.76%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F LSI+ P +YP KPPK++F TKIYHPNIN+ GNICLDILK  WSPA TLS VL++
Sbjct: 49  GVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLS 106

[Close]
BLAST of MC09g0999 vs. ExPASy Swiss-Prot
Match: Q9FKT3 (Ubiquitin-conjugating enzyme E2 30 OS=Arabidopsis thaliana OX=3702 GN=UBC30 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.4e-18
Identity = 38/58 (65.52%), Postives = 49/58 (84.48%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F ++I+ PP+YP KPPKV F+TK+YHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVTIHFPPDYPFKPPKVAFRTKVYHPNINSNGSICLDILKEQWSPALTVSKVLLS 105

[Close]
BLAST of MC09g0999 vs. NCBI nr
Match: XP_022157782.1 (ubiquitin-conjugating enzyme E2 11-like [Momordica charantia])

HSP 1 Score: 127 bits (320), Expect = 6.40e-36
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 0 

Query: 1   ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA
Sbjct: 53  ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 112

[Close]
BLAST of MC09g0999 vs. NCBI nr
Match: GAV64832.1 (UQ_con domain-containing protein [Cephalotus follicularis])

HSP 1 Score: 95.9 bits (237), Expect = 2.14e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F ++IY PP+YP KPPKVTF+TK++HPNINSKG+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVTIYFPPDYPFKPPKVTFRTKVFHPNINSKGSICLDILKEQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. NCBI nr
Match: KAG8483662.1 (hypothetical protein CXB51_022511 [Gossypium anomalum])

HSP 1 Score: 94.7 bits (234), Expect = 2.36e-23
Identity = 41/58 (70.69%), Postives = 50/58 (86.21%), Query Frame = 0 

Query: 3  GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
          G+F +SI+ PP+YP KPPKV+FKTKIYHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 19 GVFLVSIHFPPDYPFKPPKVSFKTKIYHPNINSNGSICLDILKEQWSPALTISKVLLS 76

[Close]
BLAST of MC09g0999 vs. NCBI nr
Match: KAB2065921.1 (hypothetical protein ES319_A09G124300v1 [Gossypium barbadense] >TYI10413.1 hypothetical protein ES332_A09G140100v1 [Gossypium tomentosum] >TYJ18480.1 hypothetical protein E1A91_A09G126300v1 [Gossypium mustelinum])

HSP 1 Score: 95.5 bits (236), Expect = 3.04e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F +SI+ PP+YP KPPKV+F+TK+YHPNINS G+ICLDILK  WSPAFT+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFRTKVYHPNINSNGSICLDILKEQWSPAFTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. NCBI nr
Match: TYH02494.1 (hypothetical protein ES288_A09G145100v1 [Gossypium darwinii])

HSP 1 Score: 95.5 bits (236), Expect = 3.04e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F +SI+ PP+YP KPPKV+F+TK+YHPNINS G+ICLDILK  WSPAFT+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFRTKVYHPNINSNGSICLDILKEQWSPAFTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. ExPASy TrEMBL
Match: A0A6J1DVE6 (ubiquitin-conjugating enzyme E2 11-like OS=Momordica charantia OX=3673 GN=LOC111024403 PE=3 SV=1)

HSP 1 Score: 127 bits (320), Expect = 3.10e-36
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 0 

Query: 1   ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA
Sbjct: 53  ERGLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 112

[Close]
BLAST of MC09g0999 vs. ExPASy TrEMBL
Match: A0A1Q3BAK5 (UQ_con domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_08347 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.04e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F ++IY PP+YP KPPKVTF+TK++HPNINSKG+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVTIYFPPDYPFKPPKVTFRTKVFHPNINSKGSICLDILKEQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. ExPASy TrEMBL
Match: A0A5D2P3L5 (UBC core domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_A09G140100v1 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.47e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F +SI+ PP+YP KPPKV+F+TK+YHPNINS G+ICLDILK  WSPAFT+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFRTKVYHPNINSNGSICLDILKEQWSPAFTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. ExPASy TrEMBL
Match: A0A5J5UDT4 (UBC core domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_A09G124300v1 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.47e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F +SI+ PP+YP KPPKV+F+TK+YHPNINS G+ICLDILK  WSPAFT+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFRTKVYHPNINSNGSICLDILKEQWSPAFTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. ExPASy TrEMBL
Match: A0A5D2F8V7 (UBC core domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_A09G145100v1 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.47e-23
Identity = 40/58 (68.97%), Postives = 51/58 (87.93%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 60
           G+F +SI+ PP+YP KPPKV+F+TK+YHPNINS G+ICLDILK  WSPAFT+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFRTKVYHPNINSNGSICLDILKEQWSPAFTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-20
Identity = 40/58 (68.97%), Postives = 50/58 (86.21%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F +SI+ PP+YP KPPKV+FKTK+YHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-20
Identity = 40/58 (68.97%), Postives = 50/58 (86.21%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F +SI+ PP+YP KPPKV+FKTK+YHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. TAIR 10
Match: AT2G16740.1 (ubiquitin-conjugating enzyme 29 )

HSP 1 Score: 93.2 bits (230), Expect = 7.6e-20
Identity = 38/58 (65.52%), Postives = 49/58 (84.48%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F ++I+ PP+YP KPPKV F+TK++HPNINS GNICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVNIHFPPDYPFKPPKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLS 105

[Close]
BLAST of MC09g0999 vs. TAIR 10
Match: AT5G56150.1 (ubiquitin-conjugating enzyme 30 )

HSP 1 Score: 92.0 bits (227), Expect = 1.7e-19
Identity = 38/58 (65.52%), Postives = 49/58 (84.48%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F ++I+ PP+YP KPPKV F+TK+YHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVTIHFPPDYPFKPPKVAFRTKVYHPNINSNGSICLDILKEQWSPALTVSKVLLS 105

[Close]
BLAST of MC09g0999 vs. TAIR 10
Match: AT5G56150.2 (ubiquitin-conjugating enzyme 30 )

HSP 1 Score: 92.0 bits (227), Expect = 1.7e-19
Identity = 38/58 (65.52%), Postives = 49/58 (84.48%), Query Frame = 0 

Query: 3   GLFKLSIYLPPEYPMKPPKVTFKTKIYHPNINSKGNICLDILKGNWSPAFTLSSVLVA 61
           G+F ++I+ PP+YP KPPKV F+TK+YHPNINS G+ICLDILK  WSPA T+S VL++
Sbjct: 48  GVFLVTIHFPPDYPFKPPKVAFRTKVYHPNINSNGSICLDILKEQWSPALTVSKVLLS 105

The following BLAST results are available for this feature:
BLAST of MC09g0999 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P351342.8e-1968.97Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis thaliana OX=3702 GN=UBC11 PE=1... [more]
P157328.2e-1972.41Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae (strain ATCC ... [more]
Q9SLE41.1e-1865.52Ubiquitin-conjugating enzyme E2 29 OS=Arabidopsis thaliana OX=3702 GN=UBC29 PE=2... [more]
P157311.8e-1868.97Ubiquitin-conjugating enzyme E2 4 OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
Q9FKT32.4e-1865.52Ubiquitin-conjugating enzyme E2 30 OS=Arabidopsis thaliana OX=3702 GN=UBC30 PE=1... [more]
BLAST of MC09g0999 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022157782.16.40e-36100.00ubiquitin-conjugating enzyme E2 11-like [Momordica charantia][more]
GAV64832.12.14e-2368.97UQ_con domain-containing protein [Cephalotus follicularis][more]
KAG8483662.12.36e-2370.69hypothetical protein CXB51_022511 [Gossypium anomalum][more]
KAB2065921.13.04e-2368.97hypothetical protein ES319_A09G124300v1 [Gossypium barbadense] >TYI10413.1 hypot... [more]
TYH02494.13.04e-2368.97hypothetical protein ES288_A09G145100v1 [Gossypium darwinii][more]
BLAST of MC09g0999 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DVE63.10e-36100.00ubiquitin-conjugating enzyme E2 11-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1Q3BAK51.04e-2368.97UQ_con domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_0... [more]
A0A5D2P3L51.47e-2368.97UBC core domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_A09... [more]
A0A5J5UDT41.47e-2368.97UBC core domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_A09G... [more]
A0A5D2F8V71.47e-2368.97UBC core domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_A09G1... [more]
BLAST of MC09g0999 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G08690.12.0e-2068.97ubiquitin-conjugating enzyme 11 [more]
AT3G08690.22.0e-2068.97ubiquitin-conjugating enzyme 11 [more]
AT2G16740.17.6e-2065.52ubiquitin-conjugating enzyme 29 [more]
AT5G56150.11.7e-1965.52ubiquitin-conjugating enzyme 30 [more]
AT5G56150.21.7e-1965.52ubiquitin-conjugating enzyme 30 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 1..59
e-value: 9.4E-26
score: 90.1
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..60
score: 28.487476
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 1..60
e-value: 4.28246E-28
score: 95.3469
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..60
e-value: 8.4E-29
score: 102.6
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 1..59
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..59
NoneNo IPR availablePANTHERPTHR24068:SF346BNAA01G32320D PROTEINcoord: 3..59
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 29..44

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0999.1MC09g0999.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity