MC09g0775 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0775
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	MC09: 7634695 .. 7637377 (+)
RNA-Seq Expression	MC09g0775
Synteny	MC09g0775

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGCATCAAAACAAGAACCAACGGAGGTGGTGAACAAGCATCCTCCAAGGCTCTTTAGGAGGAAAAAATGGACAAAGTTGGATATAAACTTTGCTATTTCCTTTGCCCTTTTTCATCTCCCGTGTATTTTTGCCCCGTTCTATTTCAATTGGACTGCCTTTTGGATCAGTTTCGTATTGGGTCATTCTACAGGTATAGCCATTAGTGTTTCGTATCATAGAAATCTTGCACATCGGAGCTTCAAGCTTCCTAAATGGCTCGAGTATTTGTTGGCTTATTGGGCAGCCCATGCTCTTCAGGTTCGATATCTTGGATCCTTACTCTCTTGGTATTATAAATATATGTAATGTAATAGATACCTACGACACTTAGACATCAAAAATCATAGAAGTTTTTTTTTTTTTTAAGATAAAGACAGAAGTTTCTTTTAATATTATAGAATATCATTATAGTATACAAAAATATGAATGATACATGTCATCATCAAAAGCATTGAGTTTAATAAATATTTTCTTATTCTTTTTTTTTGTTTTTTTTTTTTTTTTTGTTATTGATTGAACTATGGAAGATGAACTTGCCAAAACTAGTGACAAGTTATAGAGCATTCCCAAATATGTTTTACTAATCTTTTGTTTCGAAATATTTATTGTGTTTTAGGGTGATCCGATCGATTGGGTGAGCACGCACAGGTGTCATCATCAATATGTTGATTCAGAAAGAGACCCACATAGTCCTATTTATGGATTCTGGTTTAGTCATCTGATATGGCTTTTTGATTCCTATAATTTGACCCGAAAAGTTTGTCCGAACTATTTCAATAATTTTCAAAAGACAGAAAGAACCGTATTTACATTTTTCTTAAAGCATGGAAATCCGAGCAATGTCAATGACCTGGAGAAGCAAGCCTTTTACAGGTTCATGCGTAGAACGTACTTTCTTCATCCAATTGTTCTTGGGATATTCCTATATGCAGTCGGAGGAATTCCTTTTATTATTTGGGGAATGGTAAAATCAACTTACTCTCCTTTTTCCTTTTAACTATTAAGTTGTAATTGGATTGAATTTTATTTATTTATTTATTTATTGCTTTAGCCTATATATATATATATATATATATATNTCTATGATAGATATATGAATGAAATTTTACATTTTTACCAGAAATGGAATATTATGAGATTTGTTAAAAAGTAATGGAAATGTGAAGCTTTAATTTTAAAAGTATTTGGAAATGTGTAGTTTTACAAGAATAATTATAAATAGTATAGTTTAAAAAGTAATGACATATATAACTTTTTTTTTAATTCGTGAATCACTTGTATAATATAATTATTGTTGATTTACATTGTTGATAATTTATCTAAATAATTTTTAATTATAATTATAATCACTTGTCAATCTAAATAGGTTATGATTATAACAATCACTAATTTCACAATATTTGATTCTAGTTATATGTGTTCGATTTATTCCAACCATGTGTATGATATACTCTTTTTTTTTTCTTTTTTTTGTAATTCCCTCTGTCAAATATTTTACTTACGAAAGTCTTTTGTGTTTTTGAAACTATTCGTAGTGCATGAGGGTCGTATTACTCTTACACGTAACATCAATGGTAAATTCAGTATGCCATATATGGGGAAAGCAACCGTGGAATACTGGCGATTTGTCGAAGAATAATTGGTAACACACTTTTCCAATCTTCTCTTTTGTTCCTCTACTCATTCGCAAGTAAAAGATGATTTTTAAAAAAAGAAGTGTTTGAAATTTAATTTGGTTCCTAAACTTCCATGCTTATATTGTATCGTGAATAATTTTTTAAATATTCTATTTAAATTCCTCAAAGTTTCAAATGTTTTATTCTAGCCGTTGAATTTGGTATAAAAAAAAACTACCGAAGTCGTTTTTACCATTAGTCTTCTGCTAATCATTTCAAACATCCTAATATAAAGTTTAAAAACTAATTAAAAAAATTTTAAAATTTAGGGCTTGTTTGGTAACGTTTCTATTATTTCTTATTTTTGTTTTCGTCATTTTTAATAAAGAAAAATGAATTGCATGACCCCAAAATCCACAAATTCAAATATGATAAAATAATCTTTTTATTTGTGTTTTTTGAACAAATGGAGAATGGGAACCATATATAAGAAACTTGGTTGTTTCCAAAATTTCTATACAATCTCGAGAATGTTCCAAAAAAATGATAAACAATTAATAAACATAGTTAGTTGCAAAATGGTTACACTTCTTAAGAAAATTGGAAATTATAAAACAATAAACTAGAACGTTACCAAACGGGTCCTTAGAAATCGAAGTGGAATAAACATAACCGTCGATAAAGTTTAAATCTTTTTTTCTTTCAGGTTTGTGGCTATATTAACCTATGGAGAAGGATGGCACAATAACCACCATGCATTTGAATATTCAGCAAGACATGGTCATCGATGGTGGCAAATTGACTTTGGGTGGTATCTTATTATGTTTCTTGAAGCCATTGGAATGGCTACTGATGTGAAATTACCCTCTAAAAAGTACAAGAACCAAAAACTCCTCCTTCTTAAGCCTATGATTGATAATTAAGATATGATTAACTTAATGATATGGGTGGTTGTTTGGAAGATGGTGTAATCCAAATATATTATTATATTAATAACACTACTTTTCCTTATTATTAATCCTTCA

mRNA sequence

GAAGCATCAAAACAAGAACCAACGGAGGTGGTGAACAAGCATCCTCCAAGGCTCTTTAGGAGGAAAAAATGGACAAAGTTGGATATAAACTTTGCTATTTCCTTTGCCCTTTTTCATCTCCCGTGTATTTTTGCCCCGTTCTATTTCAATTGGACTGCCTTTTGGATCAGTTTCGTATTGGGTCATTCTACAGGTATAGCCATTAGTGTTTCGTATCATAGAAATCTTGCACATCGGAGCTTCAAGCTTCCTAAATGGCTCGAGTATTTGTTGGCTTATTGGGCAGCCCATGCTCTTCAGGGTGATCCGATCGATTGGGTGAGCACGCACAGGTGTCATCATCAATATGTTGATTCAGAAAGAGACCCACATAGTCCTATTTATGGATTCTGGTTTAGTCATCTGATATGGCTTTTTGATTCCTATAATTTGACCCGAAAAGTTTGTCCGAACTATTTCAATAATTTTCAAAAGACAGAAAGAACCGTATTTACATTTTTCTTAAAGCATGGAAATCCGAGCAATGTCAATGACCTGGAGAAGCAAGCCTTTTACAGGTTCATGCGTAGAACGTACTTTCTTCATCCAATTGTTCTTGGGATATTCCTATATGCAGTCGGAGGAATTCCTTTTATTATTTGGGGAATGTGCATGAGGGTCGTATTACTCTTACACGTAACATCAATGGTAAATTCAGTATGCCATATATGGGGAAAGCAACCGTGGAATACTGGCGATTTGTCGAAGAATAATTGGTTTGTGGCTATATTAACCTATGGAGAAGGATGGCACAATAACCACCATGCATTTGAATATTCAGCAAGACATGGTCATCGATGGTGGCAAATTGACTTTGGGTGGTATCTTATTATGTTTCTTGAAGCCATTGGAATGGCTACTGATGTGAAATTACCCTCTAAAAAGTACAAGAACCAAAAACTCCTCCTTCTTAAGCCTATGATTGATAATTAAGATATGATTAACTTAATGATATGGGTGGTTGTTTGGAAGATGGTGTAATCCAAATATATTATTATATTAATAACACTACTTTTCCTTATTATTAATCCTTCA

Coding sequence (CDS)

GAAGCATCAAAACAAGAACCAACGGAGGTGGTGAACAAGCATCCTCCAAGGCTCTTTAGGAGGAAAAAATGGACAAAGTTGGATATAAACTTTGCTATTTCCTTTGCCCTTTTTCATCTCCCGTGTATTTTTGCCCCGTTCTATTTCAATTGGACTGCCTTTTGGATCAGTTTCGTATTGGGTCATTCTACAGGTATAGCCATTAGTGTTTCGTATCATAGAAATCTTGCACATCGGAGCTTCAAGCTTCCTAAATGGCTCGAGTATTTGTTGGCTTATTGGGCAGCCCATGCTCTTCAGGGTGATCCGATCGATTGGGTGAGCACGCACAGGTGTCATCATCAATATGTTGATTCAGAAAGAGACCCACATAGTCCTATTTATGGATTCTGGTTTAGTCATCTGATATGGCTTTTTGATTCCTATAATTTGACCCGAAAAGTTTGTCCGAACTATTTCAATAATTTTCAAAAGACAGAAAGAACCGTATTTACATTTTTCTTAAAGCATGGAAATCCGAGCAATGTCAATGACCTGGAGAAGCAAGCCTTTTACAGGTTCATGCGTAGAACGTACTTTCTTCATCCAATTGTTCTTGGGATATTCCTATATGCAGTCGGAGGAATTCCTTTTATTATTTGGGGAATGTGCATGAGGGTCGTATTACTCTTACACGTAACATCAATGGTAAATTCAGTATGCCATATATGGGGAAAGCAACCGTGGAATACTGGCGATTTGTCGAAGAATAATTGGTTTGTGGCTATATTAACCTATGGAGAAGGATGGCACAATAACCACCATGCATTTGAATATTCAGCAAGACATGGTCATCGATGGTGGCAAATTGACTTTGGGTGGTATCTTATTATGTTTCTTGAAGCCATTGGAATGGCTACTGATGTGAAATTACCCTCTAAAAAGTACAAGAACCAAAAACTCCTCCTTCTTAAGCCTATGATTGATAATTAA

Protein sequence

EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSKKYKNQKLLLLKPMIDN

Homology

[Close]

BLAST of MC09g0775 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 1.6e-100
Identity = 166/304 (54.61%), Postives = 214/304 (70.39%), Query Frame = 0


Query: 10  VVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTG-IAI 69
           +V K    ++  ++W  +D          HL  + APF FNW A  ++F L   TG + I
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 70  SVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPIY 129
           ++S+HRNL+H++FKLPKWLEYL AY  A ALQG+PIDWVSTHR HHQ+ DS+RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 130 GFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRFM 189
           GFWFSH+ W+FD+  +T++                       G P+NV DLEKQ FYRF+
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 190 RRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDLS 249
           R TY LHP+ L + LYA+GG PFI+WGM +R+V + H+T +VNS CH+WGKQ WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 250 KNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSKK 309
           KNNW+VA L +GEGWHNNHHAFE+SARHG  WWQ+D  WY++ FL+AIG+ATDVKLPS+ 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 365

Query: 310 YKNQ 313
            K +
Sbjct: 384 QKQR 365

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BLAST of MC09g0775 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 338.2 bits (866), Expect = 1.0e-91
Identity = 156/309 (50.49%), Postives = 201/309 (65.05%), Query Frame = 0


Query: 8   TEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTGIA 67
           +EVV     R F  + WT  D++  + F   HL  + APFYF+W AFW+   L    GI 
Sbjct: 75  SEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC 134

Query: 68  ISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPI 127
           I++SYHRNL+HRSF LPKWLEYL AY    A QGDPI+WVS HR HH++ +++RDPHSP 
Sbjct: 135 ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPT 194

Query: 128 YGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRF 187
            GFWFSH+ W+FD+ ++                          G   NV+DL +Q FYRF
Sbjct: 195 QGFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRF 254

Query: 188 MRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDL 247
           ++RT  LH +      Y  GG+P ++WG+ + + + LH+T +VNSVCHIWG + WNT D 
Sbjct: 255 LQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDF 314

Query: 248 SKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSK 307
           SKNNW+VAILT GEGWHNNHHAFE+SARHG  WWQ+D  W LI FLEAIG+AT+VKLP++
Sbjct: 315 SKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTE 361

Query: 308 KYKNQKLLL 317
                K L+
Sbjct: 375 TQMKGKALV 361

[Close]

BLAST of MC09g0775 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 5.1e-91
Identity = 155/312 (49.68%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 7   PTEVVNKHPPR-LFRRKKWTKLD-INFAISFALFHLPCIFAPFYFNWTAFWISFVLGHST 66
           P  V  K   R +F  ++W +LD + F+ SF + H   + APFYF W+A W++F+     
Sbjct: 18  PAAVEEKKKRRWVFWDRRWRRLDYVKFSASFTV-HSLALLAPFYFTWSALWVTFLFYTIG 77

Query: 67  GIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPH 126
           G+ I+VSYHRNLAHRSFK+PKWLEYLLAY A  A+QGDPIDWVSTHR HHQ+ DSERDPH
Sbjct: 78  GLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPH 137

Query: 127 SPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAF 186
           SP  GFWFSHL+W++DS  L  K                       G  +NV DL++Q F
Sbjct: 138 SPKEGFWFSHLLWIYDSAYLVSKC----------------------GRRANVEDLKRQWF 197

Query: 187 YRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNT 246
           YRF+++T   H + LG FL+ +GG+ F+ WGM +   L +HVT ++NS+CHIWG + W T
Sbjct: 198 YRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKT 257

Query: 247 GDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKL 306
            D S+N W++++ ++GE WHNNHHAFE SAR G  WWQID  WY++ F E IG+ATDVK+
Sbjct: 258 NDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKV 306

Query: 307 PSKKYKNQKLLL 317
           P++  + +  ++
Sbjct: 318 PTEAQRRRMAIV 306

[Close]

BLAST of MC09g0775 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.5e-90
Identity = 152/292 (52.05%), Postives = 192/292 (65.75%), Query Frame = 0


Query: 22  KKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTGI-AISVSYHRNLAHRS 81
           +KW   D+   +     H+ C+FAP  F+W +FW+ F L    G+   ++S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 82  FKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPIYGFWFSHLIWLFD 141
           FKLPK+LEY  AY   HALQGDP+ WVSTHR HH+Y D+  DPHSPI GFWF H+ WLFD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 142 SYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRFMRRTYFLHPIVLG 201
           S  +  K C  Y                      N  DL KQ++YRF+ RT+  H  +  
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 202 IFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDLSKNNWFVAILTYG 261
             LY  GG PFI+WGM +R +L  H++ +VNSVCH WG +PWNTGDLS NNWF+A+LT G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 262 EGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSKKYKNQ 313
           EGWHNNHHAFEYSARHG  WWQID  WY+I  LE +G+ATD+K+PS+ +K +
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRK 307

[Close]

BLAST of MC09g0775 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.7e-89
Identity = 159/309 (51.46%), Postives = 204/309 (66.02%), Query Frame = 0


Query: 8   TEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFV-LGHSTGI 67
           +EVV +   + F R+KW   DI  A++  + H     APFYF+W AFWISF+ LG ++G+
Sbjct: 65  SEVVLERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGV 124

Query: 68  -AISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHS 127
             I++ +HR L H  FKLPK +EY  AY  + ALQGDP++WVS HR HHQ+VD+ERD HS
Sbjct: 125 LGITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHS 184

Query: 128 PIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFY 187
           P  GFWF H+ W+ D      K                     + G  +NVNDL+KQAFY
Sbjct: 185 PTQGFWFCHIGWVLDKDLFVEK---------------------RGGRRNNVNDLKKQAFY 244

Query: 188 RFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTG 247
           RF+++TY  H + L   LY VGG P+I+WGM  R+V + H T  +NSVCH WG +PWNTG
Sbjct: 245 RFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTG 304

Query: 248 DLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLP 307
           DLS NN FVA+  +GEGWHNNHHAFE SARHG  WW+ID  WY+I  L+AIG+AT+VKLP
Sbjct: 305 DLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLP 351

Query: 308 SKKYKNQKL 315
           ++  K QKL
Sbjct: 365 TEAQK-QKL 351

[Close]

BLAST of MC09g0775 vs. NCBI nr
Match: XP_022143593.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 697 bits (1800), Expect = 3.60e-254
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL
Sbjct: 2   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 61

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
           GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE
Sbjct: 62  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 121

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE
Sbjct: 122 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 181

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ
Sbjct: 182 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 241

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT
Sbjct: 242 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 301

Query: 301 DVKLPSKKYKNQKLLLLKPMIDN 323
           DVKLPSKKYKNQKLLLLKPMIDN
Sbjct: 302 DVKLPSKKYKNQKLLLLKPMIDN 324

[Close]

BLAST of MC09g0775 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 539 bits (1389), Expect = 1.90e-191
Identity = 243/327 (74.31%), Postives = 272/327 (83.18%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASK+ P E+V K PPRLFRRKKWTK D N A  F + HL CI APFYF+WT  +++  L
Sbjct: 3   EASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLALGL 62

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TG+ ISVSYHRNLAHRSFKLPKWLEY LAY A HA+QGDPIDWVSTHRCHH+YVDSE
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVDSE 122

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFS + W FDSYNLTRKVCPNYFNNFQK ER  F F++KHG+P NV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTDLE 182

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQ FYRF+ +TY+LHP+ L + LYA+GG+PFIIWGMC+R V  LHVTSMVNSVCHIWGKQ
Sbjct: 183 KQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWGKQ 242

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNW VAILT+GEGWHNNHHAFEYSARHGH WWQIDFGWY+I FL+ IG+AT
Sbjct: 243 PWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGLAT 302

Query: 301 DVKLPSKKYK-----NQKLLLLKPMID 322
           +VKLPS+K K       K   LKPMID
Sbjct: 303 NVKLPSEKQKLAALNKSKPTFLKPMID 329

[Close]

BLAST of MC09g0775 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 522 bits (1345), Expect = 9.55e-185
Identity = 236/327 (72.17%), Postives = 266/327 (81.35%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASK+E  E+V   P RLF+RKKWTK D N A  F L HL CI APFYF+WT  ++S  L
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TG+ ISVSYHRNLAHRSFKLPKWLEY LAY AAHA+QGDPIDWVSTHRCHH++VDSE
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFS + W FDSYNLTRKVCPNYFNNFQK ER  F F++KHG   NV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQ FYRF+ +TY+LHP+ L + LYA+GG+PFIIWGMC+R V+ LH TSM+NSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNW VAI T+GEGWHNNHHAFEYSARHGH WWQID+GWY+I FL+ IG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 301 DVKLPSKKYK-----NQKLLLLKPMID 322
           +VKLPS+K K       K   LKPMID
Sbjct: 303 NVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]

BLAST of MC09g0775 vs. NCBI nr
Match: XP_011659261.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8646293.1 hypothetical protein Csa_016268 [Cucumis sativus])

HSP 1 Score: 521 bits (1343), Expect = 1.93e-184
Identity = 235/327 (71.87%), Postives = 267/327 (81.65%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           E SK++P E+V K PPRLFRRKKWTK D N A  F   HL CI APFYF+WT   ++  L
Sbjct: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TG+ ISVSYHRNLAHRSFKLPKWLEY LAY A HA+QGDPIDWVSTHRCHH++VDS 
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFS + W FDSYNLTRKVCPNYFNN+QK ER +  F++KHG P NV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLE 182

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQ FYRF+ +TY+LHP+ L + LYA+GG+PFIIWGMC+R V  LHVTSMVNSVCHIWG Q
Sbjct: 183 KQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNW VA+LT+GEGWHNNHHAFEYSARHGH WWQID+GWYLI FL+ IG+AT
Sbjct: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302

Query: 301 DVKLPSKKYK-----NQKLLLLKPMID 322
           +VKLPS+K K       K + LK MID
Sbjct: 303 NVKLPSEKQKLAALNKSKPIFLKLMID 329

[Close]

BLAST of MC09g0775 vs. NCBI nr
Match: XP_022155832.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 514 bits (1325), Expect = 7.37e-182
Identity = 240/319 (75.24%), Postives = 269/319 (84.33%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASKQEP E+V K P R F RK WTKLD N A S  L HL CI APF+FNW AFWI  VL
Sbjct: 2   EASKQEPKEMV-KLPLRPFDRKTWTKLDKNIASSLFLLHLLCILAPFHFNWGAFWIFLVL 61

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TGIAISVSYH+NLAHRSFKLPKWLEYLLAY+AAHA QGDPIDWVSTHR HHQYVD+E
Sbjct: 62  TNLTGIAISVSYHKNLAHRSFKLPKWLEYLLAYYAAHAFQGDPIDWVSTHRSHHQYVDTE 121

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSP  GFWFSH+IWLFDSY LT++VCPNYFN+FQ+ ER +F  +LKHGNPSNV+DLE
Sbjct: 122 RDPHSPTNGFWFSHIIWLFDSYTLTKRVCPNYFNDFQEIERNIFMTYLKHGNPSNVHDLE 181

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQAFYRFM++TY LH   L I LY VGG+P+IIWGMCMR+V+ LH+T MVNSVCHIWGKQ
Sbjct: 182 KQAFYRFMQKTYLLHIFALAILLYFVGGVPYIIWGMCMRIVVTLHLTFMVNSVCHIWGKQ 241

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
            WNT DLSKNNW VA+LT+GEGWHNNHHAFEYSARHG  WWQID+GWYLIM  +AIG+AT
Sbjct: 242 QWNTRDLSKNNWLVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYLIMLFQAIGLAT 301

Query: 301 DVKLPSKKYKNQKLLLLKP 319
           DVKLPS+K K Q+L   +P
Sbjct: 302 DVKLPSEKQK-QRLAKTQP 318

[Close]

BLAST of MC09g0775 vs. ExPASy TrEMBL
Match: A0A6J1CR34 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111013452 PE=3 SV=1)

HSP 1 Score: 697 bits (1800), Expect = 1.74e-254
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL
Sbjct: 2   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 61

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
           GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE
Sbjct: 62  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 121

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE
Sbjct: 122 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 181

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ
Sbjct: 182 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 241

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT
Sbjct: 242 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 301

Query: 301 DVKLPSKKYKNQKLLLLKPMIDN 323
           DVKLPSKKYKNQKLLLLKPMIDN
Sbjct: 302 DVKLPSKKYKNQKLLLLKPMIDN 324

[Close]

BLAST of MC09g0775 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 524 bits (1350), Expect = 8.01e-186
Identity = 236/327 (72.17%), Postives = 268/327 (81.96%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           E SK++P E+V K PPRLFRRKKWTK D N A  F   HL CI APFYF+WT   ++  L
Sbjct: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TG+ ISVSYHRNLAHRSFKLPKWLEY LAY A HA+QGDPIDWVSTHRCHH++VDS 
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFS + W FDSYNLTRKVCPNYFNN+QK ER +F F++KHG P NV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 182

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQ FYRF+ +TY+LHP+ L + LYA+GG+PFIIWGMC+R V  LHVTSMVNSVCHIWG Q
Sbjct: 183 KQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNW VA+LT+GEGWHNNHHAFEYSARHGH WWQID+GWYLI FL+ IG+AT
Sbjct: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302

Query: 301 DVKLPSKKYK-----NQKLLLLKPMID 322
           +VKLPS+K K       K + LK MID
Sbjct: 303 NVKLPSEKQKLAALNKSKPIFLKLMID 329

[Close]

BLAST of MC09g0775 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 522 bits (1345), Expect = 4.62e-185
Identity = 236/327 (72.17%), Postives = 266/327 (81.35%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASK+E  E+V   P RLF+RKKWTK D N A  F L HL CI APFYF+WT  ++S  L
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TG+ ISVSYHRNLAHRSFKLPKWLEY LAY AAHA+QGDPIDWVSTHRCHH++VDSE
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFS + W FDSYNLTRKVCPNYFNNFQK ER  F F++KHG   NV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQ FYRF+ +TY+LHP+ L + LYA+GG+PFIIWGMC+R V+ LH TSM+NSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLSKNNW VAI T+GEGWHNNHHAFEYSARHGH WWQID+GWY+I FL+ IG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 301 DVKLPSKKYK-----NQKLLLLKPMID 322
           +VKLPS+K K       K   LKPMID
Sbjct: 303 NVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]

BLAST of MC09g0775 vs. ExPASy TrEMBL
Match: A0A6J1DQF8 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022861 PE=3 SV=1)

HSP 1 Score: 514 bits (1325), Expect = 3.57e-182
Identity = 240/319 (75.24%), Postives = 269/319 (84.33%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASKQEP E+V K P R F RK WTKLD N A S  L HL CI APF+FNW AFWI  VL
Sbjct: 2   EASKQEPKEMV-KLPLRPFDRKTWTKLDKNIASSLFLLHLLCILAPFHFNWGAFWIFLVL 61

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TGIAISVSYH+NLAHRSFKLPKWLEYLLAY+AAHA QGDPIDWVSTHR HHQYVD+E
Sbjct: 62  TNLTGIAISVSYHKNLAHRSFKLPKWLEYLLAYYAAHAFQGDPIDWVSTHRSHHQYVDTE 121

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSP  GFWFSH+IWLFDSY LT++VCPNYFN+FQ+ ER +F  +LKHGNPSNV+DLE
Sbjct: 122 RDPHSPTNGFWFSHIIWLFDSYTLTKRVCPNYFNDFQEIERNIFMTYLKHGNPSNVHDLE 181

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQAFYRFM++TY LH   L I LY VGG+P+IIWGMCMR+V+ LH+T MVNSVCHIWGKQ
Sbjct: 182 KQAFYRFMQKTYLLHIFALAILLYFVGGVPYIIWGMCMRIVVTLHLTFMVNSVCHIWGKQ 241

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
            WNT DLSKNNW VA+LT+GEGWHNNHHAFEYSARHG  WWQID+GWYLIM  +AIG+AT
Sbjct: 242 QWNTRDLSKNNWLVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYLIMLFQAIGLAT 301

Query: 301 DVKLPSKKYKNQKLLLLKP 319
           DVKLPS+K K Q+L   +P
Sbjct: 302 DVKLPSEKQK-QRLAKTQP 318

[Close]

BLAST of MC09g0775 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 7.74e-182
Identity = 232/319 (72.73%), Postives = 263/319 (82.45%), Query Frame = 0


Query: 1   EASKQEPTEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVL 60
           EASKQ P  V +  PP LF RKKWTK D N A  F L HL CI APFYF WT F+++ +L
Sbjct: 2   EASKQHPNMVQS--PPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 61

Query: 61  GHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSE 120
            + TG+AISVSYHRNLAHR+FKLPKWLEY LAY AAHA+QGDPIDWVSTHRCHH+YVD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 121 RDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLE 180
           RDPHSPIYGFWFS + W FDSYNLTRKVCPNYF NF+K ER +F FF+KHG P NV DLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 181 KQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQ 240
           KQ FYRFMR+TY+LHP+ L   LYA GGIP++IWGMC+R VL LHVTSMVNSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 241

Query: 241 PWNTGDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMAT 300
           PWNTGDLS+NNW VA++T+GEGWHNNHHAFEYSARHGH WWQID+GWY+I FL+ IG+AT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 301

Query: 301 DVKLPSKKYKNQKLLLLKP 319
            VKLPS+ +    L   KP
Sbjct: 302 CVKLPSQTHNLAALNKSKP 318

[Close]

BLAST of MC09g0775 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 367.5 bits (942), Expect = 1.1e-101
Identity = 166/304 (54.61%), Postives = 214/304 (70.39%), Query Frame = 0


Query: 10  VVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTG-IAI 69
           +V K    ++  ++W  +D          HL  + APF FNW A  ++F L   TG + I
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 70  SVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPIY 129
           ++S+HRNL+H++FKLPKWLEYL AY  A ALQG+PIDWVSTHR HHQ+ DS+RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 130 GFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRFM 189
           GFWFSH+ W+FD+  +T++                       G P+NV DLEKQ FYRF+
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 190 RRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDLS 249
           R TY LHP+ L + LYA+GG PFI+WGM +R+V + H+T +VNS CH+WGKQ WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 250 KNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSKK 309
           KNNW+VA L +GEGWHNNHHAFE+SARHG  WWQ+D  WY++ FL+AIG+ATDVKLPS+ 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 365

Query: 310 YKNQ 313
            K +
Sbjct: 384 QKQR 365

[Close]

BLAST of MC09g0775 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.3e-93
Identity = 156/309 (50.49%), Postives = 201/309 (65.05%), Query Frame = 0


Query: 8   TEVVNKHPPRLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTGIA 67
           +EVV     R F  + WT  D++  + F   HL  + APFYF+W AFW+   L    GI 
Sbjct: 75  SEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC 134

Query: 68  ISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPI 127
           I++SYHRNL+HRSF LPKWLEYL AY    A QGDPI+WVS HR HH++ +++RDPHSP 
Sbjct: 135 ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPT 194

Query: 128 YGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRF 187
            GFWFSH+ W+FD+ ++                          G   NV+DL +Q FYRF
Sbjct: 195 QGFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRF 254

Query: 188 MRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDL 247
           ++RT  LH +      Y  GG+P ++WG+ + + + LH+T +VNSVCHIWG + WNT D 
Sbjct: 255 LQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDF 314

Query: 248 SKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSK 307
           SKNNW+VAILT GEGWHNNHHAFE+SARHG  WWQ+D  W LI FLEAIG+AT+VKLP++
Sbjct: 315 SKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTE 361

Query: 308 KYKNQKLLL 317
                K L+
Sbjct: 375 TQMKGKALV 361

[Close]

BLAST of MC09g0775 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 335.9 bits (860), Expect = 3.6e-92
Identity = 155/312 (49.68%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 7   PTEVVNKHPPR-LFRRKKWTKLD-INFAISFALFHLPCIFAPFYFNWTAFWISFVLGHST 66
           P  V  K   R +F  ++W +LD + F+ SF + H   + APFYF W+A W++F+     
Sbjct: 18  PAAVEEKKKRRWVFWDRRWRRLDYVKFSASFTV-HSLALLAPFYFTWSALWVTFLFYTIG 77

Query: 67  GIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPH 126
           G+ I+VSYHRNLAHRSFK+PKWLEYLLAY A  A+QGDPIDWVSTHR HHQ+ DSERDPH
Sbjct: 78  GLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPH 137

Query: 127 SPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAF 186
           SP  GFWFSHL+W++DS  L  K                       G  +NV DL++Q F
Sbjct: 138 SPKEGFWFSHLLWIYDSAYLVSKC----------------------GRRANVEDLKRQWF 197

Query: 187 YRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNT 246
           YRF+++T   H + LG FL+ +GG+ F+ WGM +   L +HVT ++NS+CHIWG + W T
Sbjct: 198 YRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKT 257

Query: 247 GDLSKNNWFVAILTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKL 306
            D S+N W++++ ++GE WHNNHHAFE SAR G  WWQID  WY++ F E IG+ATDVK+
Sbjct: 258 NDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKV 306

Query: 307 PSKKYKNQKLLL 317
           P++  + +  ++
Sbjct: 318 PTEAQRRRMAIV 306

[Close]

BLAST of MC09g0775 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 324.7 bits (831), Expect = 8.4e-89
Identity = 146/300 (48.67%), Postives = 194/300 (64.67%), Query Frame = 0


Query: 17  RLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTGIAISVSYHRNL 76
           R    +KW +LDI  A +    H  C+ APF F W A  ++ ++    G+ I+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 77  AHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPIYGFWFSHLI 136
           AHRSFK+PKWLEY  AY    A+QGDPIDWVSTHR HHQ+ DS+RDPHSP  GFWFSHL+
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 137 WLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRFMRRTYFLHP 196
           WLFD+  L  K                       G  +NV DL++Q +Y+F++RT   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 197 IVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDLSKNNWFVAI 256
           +  G  LY  GG+ F+ WGM + V +  HVT ++NS+CH+WG + W T D S+N W++++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 257 LTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSKKYKNQKLLL 316
            ++GE WHNNHHAFE SAR G  WWQID  WY++ FLE IG+ATDVKLPS+  + +  ++
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAMV 304

[Close]

BLAST of MC09g0775 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.3e-85
Identity = 146/299 (48.83%), Postives = 191/299 (63.88%), Query Frame = 0


Query: 17  RLFRRKKWTKLDINFAISFALFHLPCIFAPFYFNWTAFWISFVLGHSTGIAISVSYHRNL 76
           R F  +KWT++D+    +    HL C+ APF + W AF  + ++G ST ++I+ SYHRNL
Sbjct: 21  RAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGISTNLSITFSYHRNL 80

Query: 77  AHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVDSERDPHSPIYGFWFSHLI 136
            HRSFKLPKWLEY  AY A  ALQG PIDWVSTHR HHQ+ DS+RDPHSPI GFWFSH+ 
Sbjct: 81  THRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHVF 140

Query: 137 WLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVNDLEKQAFYRFMRRTYFLHP 196
           W+FD+ +  R+ C                     G   NV DL++Q FYRF++ T  LH 
Sbjct: 141 WIFDT-SYIREKC---------------------GGRDNVMDLKQQWFYRFLQNTIGLHI 200

Query: 197 IVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWGKQPWNTGDLSKNNWFVAI 256
           +   I +Y  GG+P++ W + +   +  H T ++NS CHIWG + WNT D S+N W++  
Sbjct: 201 LTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWNTKDTSRNIWWLGP 260

Query: 257 LTYGEGWHNNHHAFEYSARHGHRWWQIDFGWYLIMFLEAIGMATDVKLPSKKYKNQKLL 316
            T GE WHNNHHAFE SARHG  W+Q+D  WYLI F + +G+ATDVKLP+   K +  L
Sbjct: 261 FTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLATDVKLPTDAQKRKMSL 297

The following BLAST results are available for this feature:

BLAST of MC09g0775 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	1.6e-100	54.61	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
Q9LVZ3	1.0e-91	50.49	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SID2	5.1e-91	49.68	Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=...	[more]
P0DOW2	1.5e-90	52.05	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9FV68	3.7e-89	51.46	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]

BLAST of MC09g0775 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143593.1	3.60e-254	100.00	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_038899717.1	1.90e-191	74.31	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]
XP_008451258.1	9.55e-185	72.17	PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas...	[more]
XP_011659261.2	1.93e-184	71.87	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi...	[more]
XP_022155832.1	7.37e-182	75.24	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]

BLAST of MC09g0775 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CR34	1.74e-254	100.00	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A0A0KAB1	8.01e-186	72.17	FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763...	[more]
A0A1S3BS49	4.62e-185	72.17	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DQF8	3.57e-182	75.24	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1JUL6	7.74e-182	72.73	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of MC09g0775 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	1.1e-101	54.61	fatty acid desaturase 5	[more]
AT3G15870.1	7.3e-93	50.49	Fatty acid desaturase family protein	[more]
AT2G31360.1	3.6e-92	49.68	16:0delta9 desaturase 2	[more]
AT1G06080.1	8.4e-89	48.67	delta 9 desaturase 1	[more]
AT1G06120.1	4.3e-85	48.83	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 73..93 score: 37.34 coord: 110..139 score: 48.42
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 14..314
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 50..303 e-value: 1.20376E-69 score: 212.801
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 50..284 e-value: 8.6E-17 score: 61.8
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 14..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0775.1	MC09g0775.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water