MC09g0755 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0755
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionSingle-stranded DNA-binding protein
LocationMC09: 7251887 .. 7257828 (-)
RNA-Seq ExpressionMC09g0755
SyntenyMC09g0755
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAATAAAAAAAATCTAAAACCCTTCGTCAAAACCCTGGCCCGAACAGTCTTGGCGCGCTCAAACACAGCGCAAGATAAGAGCGAGAGCGATGGTAATGGCGCTTGAACAAACCCTATCTCTTTCCTCATCGCTGTTACCAAAACACACCCTCCTCTCCATCCCAAGAAACAGATTCCGAATCGCTTCTCTCTCGCATTTAGATGCTGCGAAACGGCTCCGATTCGCCAGACGCTTCCCAAAATGCTCAGCTATGGAGAACTTCAGCAATTACTCTACATCCTACCGTAAACCTCCCGAAATTCCATGGAGTAAGGAGCTAAGCAACTCCGTAAGTCTCATCGGGACCGTTGGTACGCCTGTGGAGATCAAGCACCTAGCCTCGGGCAAGGATGTTGCTTGGACGCGGCTCGCCGTGTGGAAGTCATCTACTGAAACATCTTGGTACGAAATTCTATTGGTTTCTTTTTTGGATTGGGCTTTAGTATTGTTGCATTTTGGTAGAAGTTTGAATAAGAACGTTTACGAATATTGAAGAATAAACTCTTGAGTAGTTGGAGTTGAGATGTTAAGACTTTTTATTTGAGAATATGGGAAATTTAGGACCATGGTTAAAGACTTCGTTTACTCATCTGTTTAGGTGGGAGTGGCTCCCGAACATTTGTGTCTCAATTTCAGTATGATGTAGAAAGCATATGGATCTGTAATGGATAAACTTATTTCGTTAAAATGGCGGCTTTGCTTTACCTTCCATTCCATCTTAAAGAAATTCGGGGTTTTGACAAAAAAATGTATGAAAAATTTTAATTGCTTTTCTGTTTGAAGAATGTTGCGTCTTTTATTTGTATGAATCTATCCTACCATGAAGCCAACTTATCAATTGTAATTTGAAGGAAATACTATCTGCCAGAGCATCCTAGAACTATTTCATCCCAACAATCTCTAGACTCTGTGACCCATATCAGCCACCAAATTGCTAGCCCTTTAACCATATTTAACTGTTTAATCATCCAACATAGTTGCAATTTTGTTAATTCTGTTAGCAGTTCTAAAAATGTTGTAAGGTAATTGAGCAGAATTATAAGTCGCATGAAGTACTTGGTACTCATATAACATGGAATATACGTTTTTTTAATTAGTAACAACTTCCCCTTAAGAAAGAATAAAAAGGGAAGCAAGAAGTCATCCAAGGGCCGAAGGAGCTTGCAAATAATTACTCCAATTGACATTGATAAAGAAAGAATCATAATTGCAACAATATATGAGGTCACTAGGAGTAGGGGAAAAAAAAAAACAGAAGGCACCAAAATATGATAGAGCTCACTAGTGTCGTCCTTGAAATCCTATTCTTTTCCGGCCAAAATTTCCAAAGCAAACCAAAGCTGACATTGGTCAAAGAATCTTCCTTTTTCTCTTGAATGTGGAACTCTTACATACTTGATAGGAGCACTAGGTTAAACTAAATTGACTCTGTAAGGGGTCACCGGCCAATGAATTGACATTAGTCTATGGCATTATTTTTTTGATATCCGTGTACCTAGGCCAGCTTGGACATTAATTTATGGCATATTGATTGTCTCGCCTTTCGGACATATGTCAAACTAGCCTTTGAAAACGAGGAATTTATCCAATGCACCGAAGGAAGATAGATGAGGCCATTGTCATGTATAAATTTTCCATTGAGCTTTATAGATTTAGTCACTCCACCCAATTTAATTTTTATGAAGGAAATACAGTGATATTTAAATCACGTGCTAAGCACTAAGCTTGCTTGAATATTCTATTAATTTCCCAACTCTCTCCAAAAAAATAACTCCTTCAGTGTGTTTAGATGGCAAACCATAATACTTTTTTGAACAAGATATCAATTTTTCATTGAAAGTATGAAAAGGAACAAAAATTGTTCAAAAATACAAACTGCTGAAGGAGCGACAGAGAAAAACTAAAAGAGACAAACTTCCTAAAGAGAGCAATAAACGCGAACAAGAAATAAACGAGAGCAATAAACGCCACCCAAAGGCAGACCATAATACTTGAAAGCTACCAAATAAAATTATTGAAATTACAAAATTGAATAATTAAAGAGAATGATTTAAGAACCACTTCCTGGAACCAAATAGAACCAGAGTCCCACAAGAAAATAATACCATCTGAAGAGCCCCACAGTCCACACCTTTGATTCCTTTCACACATCCAAATACTTTTAACTAAACTAGCAGGGCAAGAAGTCTGTTTGGTTTCTTGAAGAATAATGACCTCAGGCCCCTTCCACAGTAAAACCTCGCCAAGCCTCGTTTAAAATTCCAAGGATGCTATTTGTCCACTCCCCCTTGATGACTTGGCGTGGAACCTTGGTGGACTAGACAAAGGCCTACCCATCTTGCCCAACAAAATTCTCAAGTTGGACCACTCCTCAAAAGGCAAATCTAACAGTCCCACCCCTCCTAGAACCTTGGGGAACTGGACAAAGGGCCATCCATCTTAGCCAACAAAATTCTCAGGTTAGACCACTTCTTGAAAGACAAATCTCCCACCTTGCTGATATACCTTACAGTTCCTTGGTATTAGCTAGTAAGTTCGTTAGAGGAGTGATTTTAAGTAAAGTAGCCTGGAGAGGTCAGAGATAGGCATTCTTTCAAAAAGGATTTGGGGAGAGACCAAGTCTTTCAAATTTTTAGTTACCCTTAGGCCTTATCCATTAGCAAGTCCAATCGTGTCTTTGTTGTTAATTTACTATAAGGAAAGAAAGAAAGAAAAAGGGTGGAATAAGGGATAGCATTATTGAAAGACTCTGATACTGATAGAACCTCTTGTATTATTTATGTTAAGAGAGAAACAGAGCAAGGGAGTACTTGAAGCTACCCCAAACCACAAAATAACCAAAATGAACAAGGTACCTGTTCTCTCTCTCTCACTATGGTATTTAACCATATGTACACATTCCCTCACAGCCCTACAAATTAACTGGGCCTCACCATTAAAACAGAATCACTCATCCTCACTCACATATCTTTCTTATTTTCTCTCCCCCTTCTTCCCACTATGTACTTCCAGTCTTCCACAATCGGGAGCCTATCAGACTCTTCTATTTCTTTGAAGCCAAATTTTCCTCAAAGTGCTACTTTCACTCCTTTGCATTGCAGAACTTTAACTTTGATTAGCATTTCATTCTTCATGCTTTCATATAAAACGACAATTAGAACTTAAAAGATCTTTTTGTTCTTCTAATCCATATCATCAATGAAATTGTTTCTTCTATACAAAAAACAATTAGGACTTCAGAGAGAAGAATCAGATCTAACCAGTTATTGACTAATTTTCCTCTCCTGATAATCTATGTATAAAACAATACGAATTGTTATCTTGCTGGGATTTCATTTTCCTATTCAATGTGGATAGTTGGCCCTTTCAGGATAAATTTAACATTCTGGGATGAGTTGGCACACATTGCTTTTCAACACGTCCAGAAAGGCAACCAGATACATGTATCTGGTCGGCTGGTAGCAGATCTAGTTGAAGCTGATGATGGGAAACAGCAAACATACTACAAGGTTATATAATCCTTGTTCATATCGTTCCATGGTTTTCTATTCGTTTCCTGAGGTAAATACTGATGCTTTCTACATCTTTCATAGGTAGTAGTTCAGCAGTTGAATTTTATAGAAAGAAGCATCTCAACAGCACCCCATCATGATCAGGACTCTAATTCCATAGTGGCAGGCATGGGATATTTTTGAATTTATGATTATTGATCTGACACTTTATTTTGCTTCAGAATCTTATTAGTTGATGTCCTATCATAATTTTTTAACAGGGAGAAAGCTTGGCAATGGTGCTGCTGATAATATGGGGTCCACACAAGAATTATGGCAAGCATTCTTTGCTAATCCTGTCGACTGGTGGGACAATAGGAAGAACAAGGTACTTGATTTATTCTCTTGAAATTCCTAAGATTCACGTATCTAGTTTTTGGATTCAAATGTTCTTAACATTGACGAAGTTTGTTATTTACTAGTGCTTCTATGTATATCATTGCCATTATTATATTTTGACGAGAAACAAGTAAAGCTTTTCATAGATGCGTTAAGAGAACCAAAGGAACTTACAACAAGCTGCCCAATTGGCAGAATTACAAAAAAGCTTTCATGTAAATTATGGAAAGAAGACTTCTTGTATACTGCCTATAATCTTTATCTTTAAAGATTTTTTATGCATATTTATTATTTCCTCAATGAAATTATCAGTGAAAGGAGAGAGGATTCTTATATTTGGACGAGTTTTGTAATTCAAACCTTCTTAGTTCCATTAATAGTCGAAAACACCATTAAAATTTACTTATGCTAGGATGAATAGGAGGGCCCAACAAAATGAACCCTCAGTGGGTTTCCCAAGAGATGTTAAGATTTCTTGAAGGGGGCCTGTGATGTAACTAATAACTATACCCCTAGTTCGTAATAGGATGGCGGGGAAAATTAAATAAGATATGTTAGCAGAGAGCCTTTGTGGTGGTAGGTGGGGGAATAATAGTTCTTTTAAAAAATATCTATGAGAACGGGCACGAAATAACCACTTTACCCTACAACATTTGTTGTCAAGAAAATTCGCAAGATATTAAATTTTAGGTAGGTGGCTACTTGGGGTTTTAACTCATTCTCTTTTAAGTTTCATTACTTTTTCTTTAGCACATTTGATCACTAGGCCAACTCGGGAGATATTAGGTATTTAGTTGGAAAAGCAAGAGCAGACCTCTTGAATGGCTGCATTTTCTTGGAAATCAACTATATCATGCTGCTTGCATTATCTTGGGCTCTAACAGATATTAACCGAGTGTATTACCTTTTCAAGTTTTGTTAGAACTCAAAGACACGGTACTACAACAACCTATAGTACATATCCTATTCAAGTAAGATCGTGAGCGTATTACCTTTTATGGTATGAAATCACAGAAGAGGAGGGTAGAGTCGAGTCCTGAGCCAATGAAGACAAAAAGCTCCTCCAATTAGCTGCAAGAGCATTTAAGTTATATTTGTTAAAAGAGGCATAAAAGTAATCGACCGAGTGGATTTAACCAAGAGCAACAAGGGAACAAAGGAAAGTAGTCAACTGATTTCAAAAAAAAAAAAAAAAAAAAGATTTCCACATTTTGCAGGCCACTTCAGACATTCCAAAAGTTACCTTTTGTGTGTATTTATAGGTTCTTCCCTATGGTCCTGTCTCGTTTGATAACCATTTTATTTTTTGTTTTCTGTTTTTGAAAATTAAACTTACAATTACAAATACTACTTTCACATGTGTTCTTTTATTTTCTTTTATTTTGTTATCTACCCTCTAAAAACATTTTAAAAGTCTAAACCAAATTTTTTGAACTAAAGAGAATAGTTTTTAAAAACTTGTTTGTGTTTTTGAAATTTGGCTAAGAACTGAAAAAGAAACTTGGAGGAACTAGACATCATTTTTAAAAATAGAAAACAAGAAACGAAATTGTTATCAAATGATATCAAATGAGGCCTTATATGATGCAGTTGTTGAACACTCACCCATTTGATTTTGGTTGCAGAAGAACCCTAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGTACAATCCACCTTGGGTGAAGTCCCAACTGGCAGTACTGGATATGAGAATGGGTTCTCTTGATGAACAGGAAGCCAAAATGGCTAACTTTGTTACCAATCTTGATTTCCCATCTTATTAAATCCCGACTCTGGATTCCATAAAATTGTTTCCAACCACCAAATATTTTGGTAAGATTCTCTCTGTGCTGTGGCTTGTATAGTTTGTAAATGTATCTGTTGTGGCCTGTATCTCATCATTGTTAATCATCTCTCAGTAATTTCAAATTGAAGAGAATGCCCCATAAAGAGACATTTGGAATTTTATGATATCTTGAATG

mRNA sequence

AAAAAATAAAAAAAATCTAAAACCCTTCGTCAAAACCCTGGCCCGAACAGTCTTGGCGCGCTCAAACACAGCGCAAGATAAGAGCGAGAGCGATGGTAATGGCGCTTGAACAAACCCTATCTCTTTCCTCATCGCTGTTACCAAAACACACCCTCCTCTCCATCCCAAGAAACAGATTCCGAATCGCTTCTCTCTCGCATTTAGATGCTGCGAAACGGCTCCGATTCGCCAGACGCTTCCCAAAATGCTCAGCTATGGAGAACTTCAGCAATTACTCTACATCCTACCGTAAACCTCCCGAAATTCCATGGAGTAAGGAGCTAAGCAACTCCGTAAGTCTCATCGGGACCGTTGGTACGCCTGTGGAGATCAAGCACCTAGCCTCGGGCAAGGATGTTGCTTGGACGCGGCTCGCCGTGTGGAAGTCATCTACTGAAACATCTTGGATAAATTTAACATTCTGGGATGAGTTGGCACACATTGCTTTTCAACACGTCCAGAAAGGCAACCAGATACATGTATCTGGTCGGCTGGTAGCAGATCTAGTTGAAGCTGATGATGGGAAACAGCAAACATACTACAAGGTAGTAGTTCAGCAGTTGAATTTTATAGAAAGAAGCATCTCAACAGCACCCCATCATGATCAGGACTCTAATTCCATAGTGGCAGGCAGGAGAAAGCTTGGCAATGGTGCTGCTGATAATATGGGGTCCACACAAGAATTATGGCAAGCATTCTTTGCTAATCCTGTCGACTGGTGGGACAATAGGAAGAACAAGAAGAACCCTAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGTACAATCCACCTTGGGTGAAGTCCCAACTGGCAGTACTGGATATGAGAATGGGTTCTCTTGATGAACAGGAAGCCAAAATGGCTAACTTTGTTACCAATCTTGATTTCCCATCTTATTAAATCCCGACTCTGGATTCCATAAAATTGTTTCCAACCACCAAATATTTTGGTAAGATTCTCTCTGTGCTGTGGCTTGTATAGTTTGTAAATGTATCTGTTGTGGCCTGTATCTCATCATTGTTAATCATCTCTCAGTAATTTCAAATTGAAGAGAATGCCCCATAAAGAGACATTTGGAATTTTATGATATCTTGAATG

Coding sequence (CDS)

ATGGTAATGGCGCTTGAACAAACCCTATCTCTTTCCTCATCGCTGTTACCAAAACACACCCTCCTCTCCATCCCAAGAAACAGATTCCGAATCGCTTCTCTCTCGCATTTAGATGCTGCGAAACGGCTCCGATTCGCCAGACGCTTCCCAAAATGCTCAGCTATGGAGAACTTCAGCAATTACTCTACATCCTACCGTAAACCTCCCGAAATTCCATGGAGTAAGGAGCTAAGCAACTCCGTAAGTCTCATCGGGACCGTTGGTACGCCTGTGGAGATCAAGCACCTAGCCTCGGGCAAGGATGTTGCTTGGACGCGGCTCGCCGTGTGGAAGTCATCTACTGAAACATCTTGGATAAATTTAACATTCTGGGATGAGTTGGCACACATTGCTTTTCAACACGTCCAGAAAGGCAACCAGATACATGTATCTGGTCGGCTGGTAGCAGATCTAGTTGAAGCTGATGATGGGAAACAGCAAACATACTACAAGGTAGTAGTTCAGCAGTTGAATTTTATAGAAAGAAGCATCTCAACAGCACCCCATCATGATCAGGACTCTAATTCCATAGTGGCAGGCAGGAGAAAGCTTGGCAATGGTGCTGCTGATAATATGGGGTCCACACAAGAATTATGGCAAGCATTCTTTGCTAATCCTGTCGACTGGTGGGACAATAGGAAGAACAAGAAGAACCCTAAATATCCAGATTTTAAACACAAGGACACTGGGGAAGCCTTGTGGGTTGAGGGCAGGTACAATCCACCTTGGGTGAAGTCCCAACTGGCAGTACTGGATATGAGAATGGGTTCTCTTGATGAACAGGAAGCCAAAATGGCTAACTTTGTTACCAATCTTGATTTCCCATCTTATTAA

Protein sequence

MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSNYSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY
Homology
BLAST of MC09g0755 vs. ExPASy Swiss-Prot
Match: Q9SX99 (Protein OSB1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB1 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.7e-27
Identity = 80/257 (31.13%), Postives = 127/257 (49.42%), Query Frame = 0

Query: 29  FRIASLSHLDAAKRLRFARRFPKCSAMEN----FSNYSTSYRKPPEIPWSKELSNSVSLI 88
           F++ SL    A+   + +  FPK     +      +++  ++KP        L NSVSL+
Sbjct: 5   FKLGSLIQRTAS---QISSSFPKSRFFSDGESAVYHHARLFKKPLSTKLKFNLVNSVSLM 64

Query: 89  GTVGTPVEIKHLASGKDVAWTRLAVWK--SSTETSWINLTFWDELAHIAFQHVQKGNQIH 148
           G V   +++ +    +   +T L V    +   +  I+L  WD +A     H++  + I 
Sbjct: 65  GFVDRSIQVMNTGPDRFGVFTILRVKDPLNPNRSFRISLRMWDAMARTCIAHLKLNDHIL 124

Query: 149 VSGRLVADLVEADD--GKQQTYYKVVVQQLNFIERSISTAPHHDQDSNSIVAGRRKLGNG 208
           VSGRL +    + D        Y+V V ++N+    ++  P H  DS      + K  + 
Sbjct: 125 VSGRLESYSKSSSDVYSGLNLDYQVKVAEVNY----VAAPPSHVLDSQISKNPKTKTEDD 184

Query: 209 AADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHKDTGEALWVEGRYNPPWVKSQ 268
             ++      LWQ FF+NP DWWDNR+NKKNPK PDFKHKDTGEALW+     P W+  +
Sbjct: 185 IEESKKDEIYLWQVFFSNPYDWWDNRRNKKNPKQPDFKHKDTGEALWLCSDL-PDWITRR 244

Query: 269 LAVLDMRMGSLDEQEAK 278
           L + D +    DE++ +
Sbjct: 245 LELFDQKNRFYDEEKTR 253

BLAST of MC09g0755 vs. ExPASy Swiss-Prot
Match: Q8GXH3 (Protein OSB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB2 PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.3e-21
Identity = 72/208 (34.62%), Postives = 111/208 (53.37%), Query Frame = 0

Query: 67  KPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS-WINLTFWD 126
           +P EI +  E++N V+LIG V  PV+ +  + GK  A T ++   +S  +  WI + F  
Sbjct: 87  RPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFEG 146

Query: 127 ELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIE-RSISTAPHHD 186
           +LA  A ++V K +QIHVSG+L  D    +    Q   +V+VQ LNFI+  S S +P   
Sbjct: 147 DLAKTAARYVSKDDQIHVSGKLFIDSPPPNMTYAQANVQVLVQNLNFIQPMSPSPSPFMV 206

Query: 187 QDSN-SIVAGRRKLGNGAADN--MGSTQELWQAFFANPVDWWDNRKNKKN----PKYPDF 246
             S+    +G +K    A  +  +    + W     NP +WWD+R+NK N    P++PDF
Sbjct: 207 MSSSEKEESGIKKQPARAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKPRHPDF 266

Query: 247 KHKDTGEALWVEGRYNPPWVKSQLAVLD 266
           K KD+  +LW+     P WV  +L  L+
Sbjct: 267 KSKDSSFSLWLNKA--PNWVLPKLEGLE 292

BLAST of MC09g0755 vs. ExPASy Swiss-Prot
Match: Q8GWJ4 (Protein OSB3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB3 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.1e-20
Identity = 67/204 (32.84%), Postives = 103/204 (50.49%), Query Frame = 0

Query: 68  PPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS-WINLTFWDE 127
           P +I +  E+SN ++LIG V  PV+    + GK  A T ++    S  ++ WI + F  +
Sbjct: 71  PKKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTVISQRSGSKSSNFWIPIIFEGD 130

Query: 128 LAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTAPHHDQD 187
           LA IA QHV+K ++IHVSG+L  D    +    Q+  +V+VQ LNF++ + ST       
Sbjct: 131 LAKIAVQHVKKEDRIHVSGKLFIDSPPPNVTYSQSNVQVMVQNLNFVQAATSTTKTISPP 190

Query: 188 SNSIVAGRRKLGNGAADNM--GSTQELWQAFFANPVDWWDNRKNKKN----PKYPDFKHK 247
              + + ++K        +    T   W+    NP +W D+R NK N    P +PDFK K
Sbjct: 191 EKEVTSIKKKPARSKKVKVIDEETSNSWKHLIENPKEWLDHRGNKANGLVKPGHPDFKMK 250

Query: 248 DTGEALWVEGRYNPPWVKSQLAVL 265
             G +LW+     P W   +L  L
Sbjct: 251 VGGLSLWLSTA--PDWALLKLEEL 272

BLAST of MC09g0755 vs. ExPASy Swiss-Prot
Match: Q9FYJ2 (Protein OSB4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB4 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.4e-17
Identity = 64/220 (29.09%), Postives = 108/220 (49.09%), Query Frame = 0

Query: 62  STSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS---- 121
           S  + +P E+P+  +++NS+ LIG V  PV+      GK  A T ++   SS   S    
Sbjct: 56  SEEWPRPMEVPYQPKIANSIDLIGYVHQPVQFDSTLDGKFWAGTVISHEPSSDSKSESDS 115

Query: 122 ----WINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTY----------- 181
               WI + F  +LAH A  +++K +++H++G+++ D++++     Q +           
Sbjct: 116 SSNFWIPVLFEGDLAHTANSYLKKNDRVHITGQILGDVIQSGANSDQAHVQLFKSFHGSF 175

Query: 182 -YKVVVQQLNFIERSIS---TAPHHDQD------SNSIVAGRRKLGNGAADNMGSTQELW 241
            ++V+V+ L++IE S +     P  DQ+      S S+  GR    N           LW
Sbjct: 176 SHQVMVRDLHYIEGSKAMPKVLPTLDQNEGVLKHSASVQRGREFGTN-----------LW 235

Query: 242 QAFFANPVDWWDNRKNKK----NPKYPDFKHKDTGEALWV 249
                 P +W D R+ K+    NPK+PDFK KD  +ALW+
Sbjct: 236 FDLVDKPNEWCDYREMKQNGSVNPKHPDFKKKDGSQALWL 264

BLAST of MC09g0755 vs. ExPASy Swiss-Prot
Match: Q8DCJ0 (Single-stranded DNA-binding protein OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=ssb PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 7.0e-07
Identity = 39/150 (26.00%), Postives = 71/150 (47.33%), Query Frame = 0

Query: 79  NSVSLIGTVGTPVEIKHLASGKDVA---------WTRLAVWKSSTETSWINLTFWDELAH 138
           N V L+G +G   EI+++ SG  VA         W   A  +   +T W  +T + +LA 
Sbjct: 7   NKVILVGNLGNDPEIRYMPSGGAVANITVATSETWRDKATGEPREKTEWHRVTLYGKLAE 66

Query: 139 IAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTAPHHDQDSNS 198
           +A ++++KG+Q+++ G+L     +  +G+ +   +VVVQ  N I +              
Sbjct: 67  VAGEYLRKGSQVYIEGQLQTRKWQDQNGQDRYSTEVVVQGYNGIMQ-------------- 126

Query: 199 IVAGRRKLGNGAADNMGSTQELWQAFFANP 220
           ++ GR++   G A  MG   +  Q  +  P
Sbjct: 127 MLGGRQQ--QGGAPAMGGAPQQQQGGWGQP 140

BLAST of MC09g0755 vs. NCBI nr
Match: XP_022155802.1 (protein OSB2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 584 bits (1506), Expect = 1.71e-210
Identity = 289/290 (99.66%), Postives = 289/290 (99.66%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60
           MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60

Query: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120
           YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN
Sbjct: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120

Query: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180
           LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA
Sbjct: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180

Query: 181 PHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK 240
           PHHDQDSNSIVAGR KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK
Sbjct: 181 PHHDQDSNSIVAGR-KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK 240

Query: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY
Sbjct: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 289

BLAST of MC09g0755 vs. NCBI nr
Match: XP_022155803.1 (protein OSB2, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 578 bits (1489), Expect = 6.43e-208
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60
           MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60

Query: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120
           YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN
Sbjct: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120

Query: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180
           LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA
Sbjct: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180

Query: 181 PHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK 240
           PHHDQDSNSIVAGR KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNK NPKYPDFKHK
Sbjct: 181 PHHDQDSNSIVAGR-KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNK-NPKYPDFKHK 240

Query: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY
Sbjct: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 288

BLAST of MC09g0755 vs. NCBI nr
Match: XP_038896738.1 (protein OSB2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 504 bits (1298), Expect = 9.97e-179
Identity = 246/294 (83.67%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIAS----LSHLDAAKRLRFARRFPKCSAME 60
           M+MALEQTL+LSSSLL K+++ SIPRN  RI+S    LS L+A K LRFA RFPKCSAME
Sbjct: 1   MIMALEQTLTLSSSLLSKNSIFSIPRNELRISSFYPFLSGLNAPKHLRFATRFPKCSAME 60

Query: 61  NFSNYSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTET 120
           N+S+YS +Y KP EIPW K+LSNSVSLIGTVG PVE+KHLA+GKDVAWTRLAVWKSSTET
Sbjct: 61  NYSSYSPTYPKPSEIPWKKDLSNSVSLIGTVGMPVELKHLATGKDVAWTRLAVWKSSTET 120

Query: 121 SWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERS 180
           SWINLTFWDELAH+A QH+QKGNQI+VSGRLVADLVEADDGKQQTYYKVVVQQLNFIERS
Sbjct: 121 SWINLTFWDELAHVASQHIQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERS 180

Query: 181 ISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240
            ST PH+DQDSNS++AG+ KLG+GA DN+GSTQELWQAFFANPVDWWDNRKNKKNPKYPD
Sbjct: 181 NSTGPHYDQDSNSVMAGK-KLGSGAVDNVGSTQELWQAFFANPVDWWDNRKNKKNPKYPD 240

Query: 241 FKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           FKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QEAKMANFVTN DFPSY
Sbjct: 241 FKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEAKMANFVTNFDFPSY 293

BLAST of MC09g0755 vs. NCBI nr
Match: XP_004146958.1 (protein OSB2, chloroplastic isoform X1 [Cucumis sativus] >KGN44765.1 hypothetical protein Csa_016591 [Cucumis sativus])

HSP 1 Score: 485 bits (1249), Expect = 3.01e-171
Identity = 243/295 (82.37%), Postives = 263/295 (89.15%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASL----SHLDAAKRLRFARRFPKCSAME 60
           MVMALEQTL LSSSL  K+++ SIPRN+  I+S     S+L+  K LRF   FPKCSAME
Sbjct: 1   MVMALEQTLILSSSLSSKNSIFSIPRNQLPISSFFPFHSYLNPPKHLRFPAPFPKCSAME 60

Query: 61  NFSNYST-SYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTE 120
           N++NYS  +Y KP +IPW KELSNSV+LIGTVG PVE+KHL SGKDVAWTRLAVWKSSTE
Sbjct: 61  NYTNYSAVTYPKPSQIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 120

Query: 121 TSWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180
           TSWI LTFWDELAHIA QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKVVVQQLNFIER
Sbjct: 121 TSWIGLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180

Query: 181 SISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240
           S STAPH+DQDSNSI+AGR K G+GA DN GSTQELWQAFFANPVDWWDNRKNKKNPKYP
Sbjct: 181 SNSTAPHYDQDSNSIMAGR-KPGSGAVDNTGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240

Query: 241 DFKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DFKHKDTGEALWVEGRYNPPWVKSQLA+LDMRMGSLD+QEAKMANFVT+ DFPSY
Sbjct: 241 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMGSLDDQEAKMANFVTSFDFPSY 294

BLAST of MC09g0755 vs. NCBI nr
Match: XP_008451268.1 (PREDICTED: protein OSB2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 481 bits (1239), Expect = 1.08e-169
Identity = 239/295 (81.02%), Postives = 261/295 (88.47%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASL----SHLDAAKRLRFARRFPKCSAME 60
           M +ALEQTL+LSSS   K+++ SIPRN+ RI+S     S+L+  K LRFA RFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NFSNYST-SYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTE 120
           N++NYS  +Y KP EIPW KELSNSV+LIGTVG PVE+KHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180
           TSWI+LTFWDELAHIA QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKVVVQQLNFIER
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 182

Query: 181 SISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240
           S STAPH+DQDSNSI+AGR K  +GA DN  STQELWQAFFANPVDWWDNRKNKKNPKYP
Sbjct: 183 SNSTAPHYDQDSNSIMAGR-KPSSGAVDNTASTQELWQAFFANPVDWWDNRKNKKNPKYP 242

Query: 241 DFKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DFKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QE KM NFV+N DFPSY
Sbjct: 243 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPSY 296

BLAST of MC09g0755 vs. ExPASy TrEMBL
Match: A0A6J1DQC6 (protein OSB2, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022838 PE=4 SV=1)

HSP 1 Score: 584 bits (1506), Expect = 8.27e-211
Identity = 289/290 (99.66%), Postives = 289/290 (99.66%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60
           MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60

Query: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120
           YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN
Sbjct: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120

Query: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180
           LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA
Sbjct: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180

Query: 181 PHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK 240
           PHHDQDSNSIVAGR KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK
Sbjct: 181 PHHDQDSNSIVAGR-KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK 240

Query: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY
Sbjct: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 289

BLAST of MC09g0755 vs. ExPASy TrEMBL
Match: A0A6J1DNW7 (protein OSB2, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022838 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 3.11e-208
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60
           MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN
Sbjct: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASLSHLDAAKRLRFARRFPKCSAMENFSN 60

Query: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120
           YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN
Sbjct: 61  YSTSYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETSWIN 120

Query: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180
           LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA
Sbjct: 121 LTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTA 180

Query: 181 PHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHK 240
           PHHDQDSNSIVAGR KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNK NPKYPDFKHK
Sbjct: 181 PHHDQDSNSIVAGR-KLGNGAADNMGSTQELWQAFFANPVDWWDNRKNK-NPKYPDFKHK 240

Query: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY
Sbjct: 241 DTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 288

BLAST of MC09g0755 vs. ExPASy TrEMBL
Match: A0A0A0K4W2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378540 PE=4 SV=1)

HSP 1 Score: 485 bits (1249), Expect = 1.46e-171
Identity = 243/295 (82.37%), Postives = 263/295 (89.15%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASL----SHLDAAKRLRFARRFPKCSAME 60
           MVMALEQTL LSSSL  K+++ SIPRN+  I+S     S+L+  K LRF   FPKCSAME
Sbjct: 1   MVMALEQTLILSSSLSSKNSIFSIPRNQLPISSFFPFHSYLNPPKHLRFPAPFPKCSAME 60

Query: 61  NFSNYST-SYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTE 120
           N++NYS  +Y KP +IPW KELSNSV+LIGTVG PVE+KHL SGKDVAWTRLAVWKSSTE
Sbjct: 61  NYTNYSAVTYPKPSQIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 120

Query: 121 TSWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180
           TSWI LTFWDELAHIA QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKVVVQQLNFIER
Sbjct: 121 TSWIGLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180

Query: 181 SISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240
           S STAPH+DQDSNSI+AGR K G+GA DN GSTQELWQAFFANPVDWWDNRKNKKNPKYP
Sbjct: 181 SNSTAPHYDQDSNSIMAGR-KPGSGAVDNTGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240

Query: 241 DFKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DFKHKDTGEALWVEGRYNPPWVKSQLA+LDMRMGSLD+QEAKMANFVT+ DFPSY
Sbjct: 241 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMGSLDDQEAKMANFVTSFDFPSY 294

BLAST of MC09g0755 vs. ExPASy TrEMBL
Match: A0A1S3BRV3 (protein OSB2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492617 PE=4 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 5.24e-170
Identity = 239/295 (81.02%), Postives = 261/295 (88.47%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASL----SHLDAAKRLRFARRFPKCSAME 60
           M +ALEQTL+LSSS   K+++ SIPRN+ RI+S     S+L+  K LRFA RFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NFSNYST-SYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTE 120
           N++NYS  +Y KP EIPW KELSNSV+LIGTVG PVE+KHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180
           TSWI+LTFWDELAHIA QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKVVVQQLNFIER
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 182

Query: 181 SISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240
           S STAPH+DQDSNSI+AGR K  +GA DN  STQELWQAFFANPVDWWDNRKNKKNPKYP
Sbjct: 183 SNSTAPHYDQDSNSIMAGR-KPSSGAVDNTASTQELWQAFFANPVDWWDNRKNKKNPKYP 242

Query: 241 DFKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DFKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QE KM NFV+N DFPSY
Sbjct: 243 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPSY 296

BLAST of MC09g0755 vs. ExPASy TrEMBL
Match: A0A5D3DR47 (Protein OSB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00530 PE=4 SV=1)

HSP 1 Score: 479 bits (1234), Expect = 3.02e-169
Identity = 238/295 (80.68%), Postives = 261/295 (88.47%), Query Frame = 0

Query: 1   MVMALEQTLSLSSSLLPKHTLLSIPRNRFRIASL----SHLDAAKRLRFARRFPKCSAME 60
           M +ALEQTL+LSSS   K+++ SIPRN+ RI+S     S+L+  K LRFA RFPKCSAME
Sbjct: 3   MALALEQTLTLSSSFSSKNSIFSIPRNQLRISSFFPFHSYLNPPKHLRFATRFPKCSAME 62

Query: 61  NFSNYST-SYRKPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTE 120
           N++NYS  +Y KP EIPW KELSNSV+LIGTVG PVE+KHL SGKDVAWTRLAVWKSSTE
Sbjct: 63  NYTNYSAVTYPKPSEIPWKKELSNSVTLIGTVGLPVELKHLPSGKDVAWTRLAVWKSSTE 122

Query: 121 TSWINLTFWDELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 180
           TSWI+LTFWDELAHIA QHVQKGNQI+VSGRLVADLVEADDGKQQTYYKVVVQQLNFIER
Sbjct: 123 TSWISLTFWDELAHIASQHVQKGNQIYVSGRLVADLVEADDGKQQTYYKVVVQQLNFIER 182

Query: 181 SISTAPHHDQDSNSIVAGRRKLGNGAADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYP 240
           S STAPH+DQDSNSI+AGR K  +GA DN  STQELWQAFFANPVDWWDNRKNKK+PKYP
Sbjct: 183 SNSTAPHYDQDSNSIMAGR-KPSSGAVDNTASTQELWQAFFANPVDWWDNRKNKKSPKYP 242

Query: 241 DFKHKDTGEALWVEGRYNPPWVKSQLAVLDMRMGSLDEQEAKMANFVTNLDFPSY 290
           DFKHKDTGEALWVEGRYNPPWVKSQLA+LDMRM SLD+QE KM NFV+N DFPSY
Sbjct: 243 DFKHKDTGEALWVEGRYNPPWVKSQLALLDMRMSSLDDQEVKMTNFVSNFDFPSY 296

BLAST of MC09g0755 vs. TAIR 10
Match: AT1G47720.1 (Primosome PriB/single-strand DNA-binding )

HSP 1 Score: 123.2 bits (308), Expect = 3.3e-28
Identity = 80/257 (31.13%), Postives = 127/257 (49.42%), Query Frame = 0

Query: 29  FRIASLSHLDAAKRLRFARRFPKCSAMEN----FSNYSTSYRKPPEIPWSKELSNSVSLI 88
           F++ SL    A+   + +  FPK     +      +++  ++KP        L NSVSL+
Sbjct: 5   FKLGSLIQRTAS---QISSSFPKSRFFSDGESAVYHHARLFKKPLSTKLKFNLVNSVSLM 64

Query: 89  GTVGTPVEIKHLASGKDVAWTRLAVWK--SSTETSWINLTFWDELAHIAFQHVQKGNQIH 148
           G V   +++ +    +   +T L V    +   +  I+L  WD +A     H++  + I 
Sbjct: 65  GFVDRSIQVMNTGPDRFGVFTILRVKDPLNPNRSFRISLRMWDAMARTCIAHLKLNDHIL 124

Query: 149 VSGRLVADLVEADD--GKQQTYYKVVVQQLNFIERSISTAPHHDQDSNSIVAGRRKLGNG 208
           VSGRL +    + D        Y+V V ++N+    ++  P H  DS      + K  + 
Sbjct: 125 VSGRLESYSKSSSDVYSGLNLDYQVKVAEVNY----VAAPPSHVLDSQISKNPKTKTEDD 184

Query: 209 AADNMGSTQELWQAFFANPVDWWDNRKNKKNPKYPDFKHKDTGEALWVEGRYNPPWVKSQ 268
             ++      LWQ FF+NP DWWDNR+NKKNPK PDFKHKDTGEALW+     P W+  +
Sbjct: 185 IEESKKDEIYLWQVFFSNPYDWWDNRRNKKNPKQPDFKHKDTGEALWLCSDL-PDWITRR 244

Query: 269 LAVLDMRMGSLDEQEAK 278
           L + D +    DE++ +
Sbjct: 245 LELFDQKNRFYDEEKTR 253

BLAST of MC09g0755 vs. TAIR 10
Match: AT4G20010.2 (plastid transcriptionally active 9 )

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-23
Identity = 72/208 (34.62%), Postives = 108/208 (51.92%), Query Frame = 0

Query: 67  KPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS-WINLTFWD 126
           +P EI +  E++N V+LIG V  PV+ +  + GK  A T ++   +S  +  WI + F  
Sbjct: 87  RPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFEG 146

Query: 127 ELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIE-RSISTAPHHD 186
           +LA  A ++V K +QIHVSG+L  D    +    Q   +V+VQ LNFI+  S S +P   
Sbjct: 147 DLAKTAARYVSKDDQIHVSGKLFIDSPPPNMTYAQANVQVLVQNLNFIQPMSPSPSPFMV 206

Query: 187 QDSNSIVAGRRKLGNGAADN---MGSTQELWQAFFANPVDWWDNRKNKKN----PKYPDF 246
             S+       K   G A     +    + W     NP +WWD+R+NK N    P++PDF
Sbjct: 207 MSSSEKEESGIKKQPGIAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKPRHPDF 266

Query: 247 KHKDTGEALWVEGRYNPPWVKSQLAVLD 266
           K KD+  +LW+     P WV  +L  L+
Sbjct: 267 KSKDSSFSLWLNKA--PNWVLPKLEGLE 292

BLAST of MC09g0755 vs. TAIR 10
Match: AT4G20010.1 (plastid transcriptionally active 9 )

HSP 1 Score: 105.1 bits (261), Expect = 9.4e-23
Identity = 72/208 (34.62%), Postives = 111/208 (53.37%), Query Frame = 0

Query: 67  KPPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS-WINLTFWD 126
           +P EI +  E++N V+LIG V  PV+ +  + GK  A T ++   +S  +  WI + F  
Sbjct: 87  RPNEIAYESEVANWVNLIGFVDQPVQFEASSDGKFWAGTVISQRSASDSSGFWIPIIFEG 146

Query: 127 ELAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIE-RSISTAPHHD 186
           +LA  A ++V K +QIHVSG+L  D    +    Q   +V+VQ LNFI+  S S +P   
Sbjct: 147 DLAKTAARYVSKDDQIHVSGKLFIDSPPPNMTYAQANVQVLVQNLNFIQPMSPSPSPFMV 206

Query: 187 QDSN-SIVAGRRKLGNGAADN--MGSTQELWQAFFANPVDWWDNRKNKKN----PKYPDF 246
             S+    +G +K    A  +  +    + W     NP +WWD+R+NK N    P++PDF
Sbjct: 207 MSSSEKEESGIKKQPARAKQDIVIDEASDSWNHLIENPKEWWDHRENKVNGLVKPRHPDF 266

Query: 247 KHKDTGEALWVEGRYNPPWVKSQLAVLD 266
           K KD+  +LW+     P WV  +L  L+
Sbjct: 267 KSKDSSFSLWLNKA--PNWVLPKLEGLE 292

BLAST of MC09g0755 vs. TAIR 10
Match: AT5G44785.2 (organellar single-stranded DNA binding protein 3 )

HSP 1 Score: 102.1 bits (253), Expect = 7.9e-22
Identity = 67/204 (32.84%), Postives = 103/204 (50.49%), Query Frame = 0

Query: 68  PPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS-WINLTFWDE 127
           P +I +  E+SN ++LIG V  PV+    + GK  A T ++    S  ++ WI + F  +
Sbjct: 71  PKKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTVISQRSGSKSSNFWIPIIFEGD 130

Query: 128 LAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTAPHHDQD 187
           LA IA QHV+K ++IHVSG+L  D    +    Q+  +V+VQ LNF++ + ST       
Sbjct: 131 LAKIAVQHVKKEDRIHVSGKLFIDSPPPNVTYSQSNVQVMVQNLNFVQAATSTTKTISPP 190

Query: 188 SNSIVAGRRKLGNGAADNM--GSTQELWQAFFANPVDWWDNRKNKKN----PKYPDFKHK 247
              + + ++K        +    T   W+    NP +W D+R NK N    P +PDFK K
Sbjct: 191 EKEVTSIKKKPARSKKVKVIDEETSNSWKHLIENPKEWLDHRGNKANGLVKPGHPDFKMK 250

Query: 248 DTGEALWVEGRYNPPWVKSQLAVL 265
             G +LW+     P W   +L  L
Sbjct: 251 VGGLSLWLSTA--PDWALLKLEEL 272

BLAST of MC09g0755 vs. TAIR 10
Match: AT5G44785.1 (organellar single-stranded DNA binding protein 3 )

HSP 1 Score: 102.1 bits (253), Expect = 7.9e-22
Identity = 67/204 (32.84%), Postives = 103/204 (50.49%), Query Frame = 0

Query: 68  PPEIPWSKELSNSVSLIGTVGTPVEIKHLASGKDVAWTRLAVWKSSTETS-WINLTFWDE 127
           P +I +  E+SN ++LIG V  PV+    + GK  A T ++    S  ++ WI + F  +
Sbjct: 71  PKKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTVISQRSGSKSSNFWIPIIFEGD 130

Query: 128 LAHIAFQHVQKGNQIHVSGRLVADLVEADDGKQQTYYKVVVQQLNFIERSISTAPHHDQD 187
           LA IA QHV+K ++IHVSG+L  D    +    Q+  +V+VQ LNF++ + ST       
Sbjct: 131 LAKIAVQHVKKEDRIHVSGKLFIDSPPPNVTYSQSNVQVMVQNLNFVQAATSTTKTISPP 190

Query: 188 SNSIVAGRRKLGNGAADNM--GSTQELWQAFFANPVDWWDNRKNKKN----PKYPDFKHK 247
              + + ++K        +    T   W+    NP +W D+R NK N    P +PDFK K
Sbjct: 191 EKEVTSIKKKPARSKKVKVIDEETSNSWKHLIENPKEWLDHRGNKANGLVKPGHPDFKMK 250

Query: 248 DTGEALWVEGRYNPPWVKSQLAVL 265
             G +LW+     P W   +L  L
Sbjct: 251 VGGLSLWLSTA--PDWALLKLEEL 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX994.7e-2731.13Protein OSB1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB1 PE=1 SV=1[more]
Q8GXH31.3e-2134.62Protein OSB2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB2 PE=1 SV=2[more]
Q8GWJ41.1e-2032.84Protein OSB3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OSB... [more]
Q9FYJ24.4e-1729.09Protein OSB4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OSB4 PE=2 SV=1[more]
Q8DCJ07.0e-0726.00Single-stranded DNA-binding protein OS=Vibrio vulnificus (strain CMCP6) OX=21689... [more]
Match NameE-valueIdentityDescription
XP_022155802.11.71e-21099.66protein OSB2, chloroplastic-like isoform X1 [Momordica charantia][more]
XP_022155803.16.43e-20899.31protein OSB2, chloroplastic-like isoform X2 [Momordica charantia][more]
XP_038896738.19.97e-17983.67protein OSB2, chloroplastic-like [Benincasa hispida][more]
XP_004146958.13.01e-17182.37protein OSB2, chloroplastic isoform X1 [Cucumis sativus] >KGN44765.1 hypothetica... [more]
XP_008451268.11.08e-16981.02PREDICTED: protein OSB2, chloroplastic-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1DQC68.27e-21199.66protein OSB2, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1DNW73.11e-20899.31protein OSB2, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0K4W21.46e-17182.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G378540 PE=4 SV=1[more]
A0A1S3BRV35.24e-17081.02protein OSB2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492617 PE=4 SV... [more]
A0A5D3DR473.02e-16980.68Protein OSB2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00530 P... [more]
Match NameE-valueIdentityDescription
AT1G47720.13.3e-2831.13Primosome PriB/single-strand DNA-binding [more]
AT4G20010.22.5e-2334.62plastid transcriptionally active 9 [more]
AT4G20010.19.4e-2334.62plastid transcriptionally active 9 [more]
AT5G44785.27.9e-2232.84organellar single-stranded DNA binding protein 3 [more]
AT5G44785.17.9e-2232.84organellar single-stranded DNA binding protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.40.50.140coord: 75..181
e-value: 2.3E-20
score: 74.8
NoneNo IPR availablePANTHERPTHR10302:SF15OS03G0633900 PROTEINcoord: 29..282
IPR000424Primosome PriB/single-strand DNA-bindingPFAMPF00436SSBcoord: 79..172
e-value: 3.5E-16
score: 59.1
IPR000424Primosome PriB/single-strand DNA-bindingPROSITEPS50935SSBcoord: 77..174
score: 21.196428
IPR000424Primosome PriB/single-strand DNA-bindingCDDcd04496SSB_OBFcoord: 81..173
e-value: 7.80248E-19
score: 77.6525
IPR011344Single-stranded DNA-binding proteinTIGRFAMTIGR00621TIGR00621coord: 79..186
e-value: 1.1E-15
score: 56.3
IPR011344Single-stranded DNA-binding proteinPANTHERPTHR10302SINGLE-STRANDED DNA-BINDING PROTEINcoord: 29..282
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 79..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0755.1MC09g0755.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006264 mitochondrial DNA replication
biological_process GO:0051096 positive regulation of helicase activity
biological_process GO:0006260 DNA replication
cellular_component GO:0042645 mitochondrial nucleoid
molecular_function GO:0003697 single-stranded DNA binding