MC09g0730 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0730
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionsucrose-binding protein-like
LocationMC09: 6846476 .. 6849632 (+)
RNA-Seq ExpressionMC09g0730
SyntenyMC09g0730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCCAAAATCAAGCTCTCTCTGCCGCTGCTGCTCCTCCTCGTCCTCTCTGTCGCCGTGCTGGCCGGCAAGGACCCGGACCTGAAGCAGTGCCGGAGCCAGTGCAAACTCAAGCGGGAGACCGCCGAGACACAGAAGCGAGACTGCGAGAAAAGGTGCGTGTGCGAGGAAATGAAGAAGAAGAAGAATGAGAGCGAAGGAGGAAAAACCAACCAGATTTCAGGAGAAGAAACCGTCGCCGCCGCCCTGGCCGGCAAAGACCCCGCCACGAAGCAGTGCCGGAGCCAGTGCAAACTCGAGCTGGAGATCGCCGAGAGACAGAGACGAGACTGCGAGAAAAGGTGCGAAGAAACGAAGAAGAAGAAGAAGAAGAAGGAGAAGGAGAACGAAGGAGGAAGAACCAACCAGATTTCAGAAGTAGAAGGAGAAGAAACAGAGTATGAGGAGAGAGAGAAAGAGGAGGAGAATCCGTATGTGTTCGACAACGAACATTTCAAAGCTAGGATTGAAACCGAGGAAGGCACGGTTAGGGTTCTTCCCAAATTCACCCAGAAATCCCAACTTCTCCGCGGGATTGAGAACTTCCGGATCTCGATTCTCGAAGCCAATCACTCGACCTTCGTGGCTCCCAGCCATTTCGATGCCGAGATTATTCTGTTCGTTGCTCAGGGTATGTTGTGTGATTTCTGCCTTCTTGGGTGTCGAATTGTTGCGCGGAAGGTGTTTGTGAAAATGATTCTTTGGGGATTTTTGGTGCAGGGCGGGCTACGGTTACCGTTATCAGGGAGAGGAGAGCGAGCTTCGAACTGCAACCTGGAGATGTCTTCAGAGTTCCATCTGGAGCTCCGTTTTATTTGATAAACAAGGACGAACGTGAGAAACTGAGGATTTTTAATCTTCTTCAAGCCACCTCCATTCCTGGACAACTTGAGGTAGCCAAAGCTTTTCTGTGCATTGGTCTTTATACGTCTTTTTATTACTTTGAAGGTTAAGTTATGAATTAATTTGGTCCTGTGACTTGGATATAATTTTGGACATAATTGTAGGGACAATCGACAAATTGGAAGTATCCTCAAACAATAGGGACAGATTTTTGTAACTTAATCAAATTCTAAATTATGGGGTCAACTTCTATTTCATGTGAATGAATATTTAGAATTTGGGCAGGTTTTTCATGGGCCAGGTGGAGAAGACCCAGAATCATTCTACACAGCATTCAGCTGGGAGTTGCTTGAAGCAGCCTTGAAGGTAATCAAAATTCTTTTTCCAAACTGTGTAAACAGTGCGCCTGTCATACATTTTCCCTCATACTTTCTCGGCTGCCAAACAGGTTCCAAGAGACGAGCTGGAGAGATTTTTCAAGCAACAAAAAGGAGGGGCCATTATGAAGGCTTCTAGAGAACAGATCCAATCACTTAGCCAACACGAAGAGATCATCCCAAGGATCTGGCCCTTCTCAGAAGGCAAAACCGAGCGCCCATTCAATCTATTCAAGCAAAGTCCATGGCAGTCGAACAAGTTCGGTCACTTCCACATAGGCCACCCTCACGAGTTCAGCCAGCTCCAAGACCTCGGCATTGCCATCTCCTTTGCCGACTTCACAGAAGTAATTACCTGTTCCCTCTCATTTGAAATGCTGGATATATTTTTTATCTTCTAAATATTTGTTAAATTACAAAGTTGCTGAAACTTTACGGCCTATTAGTCTTCATAGACCCTTTTGAAGTATTATGAAATCTGACTATTCTAGAGAAGGGAAGAAAGTTTCTGATTCTTCTTATTCTCCTAAATTTGCATCACACGATACTGATAATGAGTGAGTCATGAGTGAGTGAGTAGACATTTAGAAGAATCTAAGTCATATCTTCCCTTCAAAAATTCAGAAGAATTAGGTCAATATCCCCCCTCCAAATTGGCAGAATTTGACTTTGGAACTCACTCACTAATTATAACTATAATCATCATATCATATTTATGAAAAATGTCAAAACATAATTGAAAAAAATCTGAGATTAAGAATTTGAAATCACGAAAATCATAAAACAAATTATTATTCAGCAGCTGAGCTTATTTTTGCTGTGTGTTTTTGGGAGGGTTCAATGATGGCTCCCCACTACAACTCCAAAGCTATGACGATAGGGGTGGTGGTAGACGGTGGAGGGGGGTTTCAAATGGCGTGTCCCCACCTTGGGCGCCGTGGACAGAGGGGTGAGTCCAGTTACCAGAAAATAAGAGGGGTGCTGAGACGTGATGTGGTGATCATAGCTCCAGCAGGGCATCCATTTTCAATCTTCGCTTCACCGGGACATGGTCTCCGGATCGTCTTCTTTGAAATCAATGCCGATGGCAATATCAAGTATCTTCTTGCAGGTAAAAACGACTACACCATAAAACCATCCAACAAAGTTTTTTTTAAACCCAATGTAGCCTAAAATAGACTGAAAAAACTGCAGGGAAAGAGAACATAGTAAACAAGATGGAGAGAATAGCAAGAGGGTTGGGATTCGGCACAGCAGGAAAAGAGGTGAAGAGGATCTTCAGACATCAAGAGGAGGAGTTTTTCTTCCCAGGGCCGACCCAGCTGCAGGAAGAACGTGGTCAACATCAATGGGCTGATGCCTGAGTGAGGAACAACCCAAGAGACAAGTGTTAAATAAACGGGTATGGTACAGTTTAAATTTACTACTCCTAGGCTCTACTCCCTTGTACAGTGTACAGATTACAGACTTTGCATAAATAAAGATGAGTTGGGGTTTGCGTAGATGAATGAATCAGGGTTTAAGTTTTGTTTTGGCGTCGATTACATTTAATTTTGAGAAAAGAGATGAACAGTGCCCCTACTTTCAGGGGCTAGTTTGCTTCGTTTCTGGTTGACTTTTTTAATTTCGAGTGGTTGGTAGTGCTGAGGTGGTGAGAGGTTGAAGAAGATTGGTTCAGAAAACAATAAAATCATAATCAGAGATTCTCAGATTGGCTGCGGCTGCTATGGAAACCAAAGCTTATGCTCATGGCTGTAAATTCCAAGTTTTGTGATATGTCCATGAGGAAATTTTGACTGGTGATATGTTTTTTATTTTTATCTGTACGAGTGAAAAAGCATATTTGAAACAGTTTTGTGTTGAATTGGAACCAAATTACAGGATCTTAGTTTTGG

mRNA sequence

ATGGCTCCCAAAATCAAGCTCTCTCTGCCGCTGCTGCTCCTCCTCGTCCTCTCTGTCGCCGTGCTGGCCGGCAAGGACCCGGACCTGAAGCAGTGCCGGAGCCAGTGCAAACTCAAGCGGGAGACCGCCGAGACACAGAAGCGAGACTGCGAGAAAAGGTGCGAAGAAACGAAGAAGAAGAAGAAGAAGAAGGAGAAGGAGAACGAAGGAGGAAGAACCAACCAGATTTCAGAAGTAGAAGGAGAAGAAACAGAGTATGAGGAGAGAGAGAAAGAGGAGGAGAATCCGTATGTGTTCGACAACGAACATTTCAAAGCTAGGATTGAAACCGAGGAAGGCACGGTTAGGGTTCTTCCCAAATTCACCCAGAAATCCCAACTTCTCCGCGGGATTGAGAACTTCCGGATCTCGATTCTCGAAGCCAATCACTCGACCTTCGTGGCTCCCAGCCATTTCGATGCCGAGATTATTCTGTTCGTTGCTCAGGGGCGGGCTACGGTTACCGTTATCAGGGAGAGGAGAGCGAGCTTCGAACTGCAACCTGGAGATGTCTTCAGAGTTCCATCTGGAGCTCCGTTTTATTTGATAAACAAGGACGAACGTGAGAAACTGAGGATTTTTAATCTTCTTCAAGCCACCTCCATTCCTGGACAACTTGAGGTTTTTCATGGGCCAGGTGGAGAAGACCCAGAATCATTCTACACAGCATTCAGCTGGGAGTTGCTTGAAGCAGCCTTGAAGGTTCCAAGAGACGAGCTGGAGAGATTTTTCAAGCAACAAAAAGGAGGGGCCATTATGAAGGCTTCTAGAGAACAGATCCAATCACTTAGCCAACACGAAGAGATCATCCCAAGGATCTGGCCCTTCTCAGAAGGCAAAACCGAGCGCCCATTCAATCTATTCAAGCAAAGTCCATGGCAGTCGAACAAGTTCGGTCACTTCCACATAGGCCACCCTCACGAGTTCAGCCAGCTCCAAGACCTCGGCATTGCCATCTCCTTTGCCGACTTCACAGAAGGTTCAATGATGGCTCCCCACTACAACTCCAAAGCTATGACGATAGGGGTGGTGGTAGACGGTGGAGGGGGGTTTCAAATGGCGTGTCCCCACCTTGGGCGCCGTGGACAGAGGGGTGAGTCCAGTTACCAGAAAATAAGAGGGGTGCTGAGACGTGATGTGGTGATCATAGCTCCAGCAGGGCATCCATTTTCAATCTTCGCTTCACCGGGACATGGTCTCCGGATCGTCTTCTTTGAAATCAATGCCGATGGCAATATCAAGTATCTTCTTGCAGGGAAAGAGAACATAGTAAACAAGATGGAGAGAATAGCAAGAGGGTTGGGATTCGGCACAGCAGGAAAAGAGGTGAAGAGGATCTTCAGACATCAAGAGGAGGAGTTTTTCTTCCCAGGGCCGACCCAGCTGCAGGAAGAACGTGGTCAACATCAATGGGCTGATGCCTGAGTGAGGAACAACCCAAGAGACAAGTGTTAAATAAACGGGTATGGTACAGTTTAAATTTACTACTCCTAGGCTCTACTCCCTTGTACAGTGTACAGATTACAGACTTTGCATAAATAAAGATGAGTTGGGGTTTGCGTAGATGAATGAATCAGGGTTTAAGTTTTGTTTTGGCGTCGATTACATTTAATTTTGAGAAAAGAGATGAACAGTGCCCCTACTTTCAGGGGCTAGTTTGCTTCGTTTCTGGTTGACTTTTTTAATTTCGAGTGGTTGGTAGTGCTGAGGTGGTGAGAGGTTGAAGAAGATTGGTTCAGAAAACAATAAAATCATAATCAGAGATTCTCAGATTGGCTGCGGCTGCTATGGAAACCAAAGCTTATGCTCATGGCTGTAAATTCCAAGTTTTGTGATATGTCCATGAGGAAATTTTGACTGGTGATATGTTTTTTATTTTTATCTGTACGAGTGAAAAAGCATATTTGAAACAGTTTTGTGTTGAATTGGAACCAAATTACAGGATCTTAGTTTTGG

Coding sequence (CDS)

ATGGCTCCCAAAATCAAGCTCTCTCTGCCGCTGCTGCTCCTCCTCGTCCTCTCTGTCGCCGTGCTGGCCGGCAAGGACCCGGACCTGAAGCAGTGCCGGAGCCAGTGCAAACTCAAGCGGGAGACCGCCGAGACACAGAAGCGAGACTGCGAGAAAAGGTGCGAAGAAACGAAGAAGAAGAAGAAGAAGAAGGAGAAGGAGAACGAAGGAGGAAGAACCAACCAGATTTCAGAAGTAGAAGGAGAAGAAACAGAGTATGAGGAGAGAGAGAAAGAGGAGGAGAATCCGTATGTGTTCGACAACGAACATTTCAAAGCTAGGATTGAAACCGAGGAAGGCACGGTTAGGGTTCTTCCCAAATTCACCCAGAAATCCCAACTTCTCCGCGGGATTGAGAACTTCCGGATCTCGATTCTCGAAGCCAATCACTCGACCTTCGTGGCTCCCAGCCATTTCGATGCCGAGATTATTCTGTTCGTTGCTCAGGGGCGGGCTACGGTTACCGTTATCAGGGAGAGGAGAGCGAGCTTCGAACTGCAACCTGGAGATGTCTTCAGAGTTCCATCTGGAGCTCCGTTTTATTTGATAAACAAGGACGAACGTGAGAAACTGAGGATTTTTAATCTTCTTCAAGCCACCTCCATTCCTGGACAACTTGAGGTTTTTCATGGGCCAGGTGGAGAAGACCCAGAATCATTCTACACAGCATTCAGCTGGGAGTTGCTTGAAGCAGCCTTGAAGGTTCCAAGAGACGAGCTGGAGAGATTTTTCAAGCAACAAAAAGGAGGGGCCATTATGAAGGCTTCTAGAGAACAGATCCAATCACTTAGCCAACACGAAGAGATCATCCCAAGGATCTGGCCCTTCTCAGAAGGCAAAACCGAGCGCCCATTCAATCTATTCAAGCAAAGTCCATGGCAGTCGAACAAGTTCGGTCACTTCCACATAGGCCACCCTCACGAGTTCAGCCAGCTCCAAGACCTCGGCATTGCCATCTCCTTTGCCGACTTCACAGAAGGTTCAATGATGGCTCCCCACTACAACTCCAAAGCTATGACGATAGGGGTGGTGGTAGACGGTGGAGGGGGGTTTCAAATGGCGTGTCCCCACCTTGGGCGCCGTGGACAGAGGGGTGAGTCCAGTTACCAGAAAATAAGAGGGGTGCTGAGACGTGATGTGGTGATCATAGCTCCAGCAGGGCATCCATTTTCAATCTTCGCTTCACCGGGACATGGTCTCCGGATCGTCTTCTTTGAAATCAATGCCGATGGCAATATCAAGTATCTTCTTGCAGGGAAAGAGAACATAGTAAACAAGATGGAGAGAATAGCAAGAGGGTTGGGATTCGGCACAGCAGGAAAAGAGGTGAAGAGGATCTTCAGACATCAAGAGGAGGAGTTTTTCTTCCCAGGGCCGACCCAGCTGCAGGAAGAACGTGGTCAACATCAATGGGCTGATGCCTGA

Protein sequence

MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKKKKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNLFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERGQHQWADA
Homology
BLAST of MC09g0730 vs. ExPASy Swiss-Prot
Match: A0A2I4E5L6 (Vicilin Jug r 6.0101 OS=Juglans regia OX=51240 GN=LOC108986502 PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.8e-138
Identity = 254/496 (51.21%), Postives = 350/496 (70.56%), Query Frame = 0

Query: 3   PKIKLSLPLL--LLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 62
           PKI ++L LL  LL + +   LA +DP+LKQC+ QC+ +R+  E +K  C++ C+E   +
Sbjct: 5   PKIPIALLLLTSLLAICAGLALAMQDPELKQCKHQCRHQRQFDEQEKEHCQRSCDEYHIE 64

Query: 63  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 122
           KK +E+          S  EG E E    E+EEENPYVF++E F+ R+ T+EG ++VL K
Sbjct: 65  KKARERAERRRSEEGSSREEGYEEEELGGEREEENPYVFEDEDFETRVRTDEGRIQVLEK 124

Query: 123 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIR-ERRASFEL 182
           FT++S+LLRGIENFR++ILEAN  TF++P+HFDAE+++FVA+GRAT+T +R E+R +F +
Sbjct: 125 FTKRSKLLRGIENFRVAILEANPQTFISPAHFDAELVVFVAKGRATITTVREEKRENFNV 184

Query: 183 QPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSW 242
           + GD+ R+P+G P YLIN+DE EKL I  +L+  S+PG  E FHG GGEDPESFY AFSW
Sbjct: 185 EQGDIMRIPAGTPVYLINRDENEKLYIVKILRPVSVPGHFEAFHGSGGEDPESFYRAFSW 244

Query: 243 ELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFN 302
           E+LEAALK  RD+LE+ F +Q  G I+KAS+EQI+S+S+HEE  PRIWPF  G +  PFN
Sbjct: 245 EVLEAALKTRRDQLEKLFGKQTQGVIIKASKEQIRSMSKHEETTPRIWPFG-GDSTHPFN 304

Query: 303 LFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVD 362
           LF + P QSN+FG      P E  QLQDL + +SFA+ T+GSM  P+YNS+A  I VV++
Sbjct: 305 LFHKRPSQSNQFGRLFETDPKECKQLQDLDLMVSFANITKGSMAGPYYNSRATKISVVIE 364

Query: 363 GGGGFQMACPHLGRRGQRGE--------------SSYQKIRGVLRRDVVIIAPAGHPFSI 422
           G G F+MACPHL   G RG+               SYQ+IRG LR  +V +APAGHP ++
Sbjct: 365 GEGYFEMACPHLSSSGSRGQREGSGSSRRRSRSGPSYQQIRGRLRPGMVFVAPAGHPVAV 424

Query: 423 FASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEE 482
            AS    L+++ F++NA GNI++ LAGK NIVN+ E+ A+ L F    +EV++IFR+Q++
Sbjct: 425 IASRNKNLQVLCFDVNAQGNIRFPLAGKNNIVNEFEKEAKELAFNFPAREVEKIFRNQDQ 484

BLAST of MC09g0730 vs. ExPASy Swiss-Prot
Match: Q8S4P9 (Vicilin Cor a 11.0101 OS=Corylus avellana OX=13451 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 8.1e-133
Identity = 242/462 (52.38%), Postives = 335/462 (72.51%), Query Frame = 0

Query: 21  VLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKKKKKKEKENEGGRTNQISEVE 80
           +L  +DP+LK+C+ +C+ +R+  E Q+RD ++ CEE     K +E++ E          E
Sbjct: 1   MLPKEDPELKKCKHKCRDERQFDEQQRRDGKQICEE-----KARERQQE----------E 60

Query: 81  GEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPKFTQKSQLLRGIENFRISILE 140
           G  +E    +++EENPYVF +EHF++R++TEEG V+VL  FT++S+LL GIENFR++ILE
Sbjct: 61  GNSSEESYGKEQEENPYVFQDEHFESRVKTEEGRVQVLENFTKRSRLLSGIENFRLAILE 120

Query: 141 ANHSTFVAPSHFDAEIILFVAQGRATVTVIR-ERRASFELQPGDVFRVPSGAPFYLINKD 200
           AN  TF++P+HFDAE++LFVA+GRAT+T++R E+R SF ++ GD+ R+P+G P Y+IN+D
Sbjct: 121 ANPHTFISPAHFDAELVLFVAKGRATITMVREEKRESFNVEHGDIIRIPAGTPVYMINRD 180

Query: 201 EREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWELLEAALKVPRDELERFFKQ 260
           E EKL I  +LQ  S PG  E F+G GGEDPESFY AFSWE+LEAALKV R++LE+ F +
Sbjct: 181 ENEKLFIVKILQPVSAPGHFEAFYGAGGEDPESFYRAFSWEVLEAALKVRREQLEKVFGE 240

Query: 261 QKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNLFKQSPWQSNKFGHFHIGHP 320
           Q  G+I+KASRE+I++LSQHEE  PRIWPF  G++  P NL  + P QSN+FG  +  HP
Sbjct: 241 QSKGSIVKASREKIRALSQHEEGPPRIWPFG-GESSGPINLLHKHPSQSNQFGRLYEAHP 300

Query: 321 HEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMACPHLGRRGQRGE 380
            +  QLQDL + +SFA+ T+GSM  P+YNS+A  I VVV+G G F+MACPHL        
Sbjct: 301 DDHKQLQDLDLMVSFANITKGSMAGPYYNSRATKISVVVEGEGFFEMACPHL----SSSS 360

Query: 381 SSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINADGNIKYLLAGKENIVNK 440
            SYQKI   LRR VV +APAGHP ++ AS  + L+++ FE+NA GN ++ LAGK NIVN+
Sbjct: 361 GSYQKISARLRRGVVFVAPAGHPVAVIASQNNNLQVLCFEVNAHGNSRFPLAGKGNIVNE 420

Query: 441 MERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERGQ 482
            ER A+ L F    +EV+RIF++Q++ FFFPGP + QEE G+
Sbjct: 421 FERDAKELAFNLPSREVERIFKNQDQAFFFPGPNKQQEEGGR 442

BLAST of MC09g0730 vs. ExPASy Swiss-Prot
Match: B4X640 (Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2)

HSP 1 Score: 426.4 bits (1095), Expect = 4.3e-118
Identity = 242/544 (44.49%), Postives = 333/544 (61.21%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVA---VLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEET 60
           M  + K  L L L+ VL +     LA  DP+LKQC+ QCK++R+  E QK  C K CE+ 
Sbjct: 1   MGSRTKFCLTLFLVSVLILCAGLALAKTDPELKQCKHQCKVQRQYDEEQKEQCAKGCEKY 60

Query: 61  KKKKKKKEKENE------------------------------------GGRTNQI----- 120
            K+KK +E+E E                                    GG+  Q+     
Sbjct: 61  YKEKKGREQEEEEEEEWGSGRGRGDEFSTHEPGEKRLSQCMKQCERQDGGQQKQLCRFRC 120

Query: 121 --------------SEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPKFT 180
                          + E EE   EE+E+E+ENPYVF++EHF  R++TE+G V VLPKFT
Sbjct: 121 QEKYKKERREHSYSRDEEEEEEGDEEQEEEDENPYVFEDEHFTTRVKTEQGKVVVLPKFT 180

Query: 181 QKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRE-RRASFELQP 240
           ++S+LLRG+E +R++ L AN   FV P+H DA+ I FV+ GR T+T IRE +R S  ++ 
Sbjct: 181 KRSKLLRGLEKYRLAFLVANPQAFVVPNHMDADSIFFVSWGRGTITKIRENKRESMNVKQ 240

Query: 241 GDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWEL 300
           GD+ R+ +G PFY++N DE EKL I  LLQ  ++PG  EVFHGPGGE+PESFY AFS E+
Sbjct: 241 GDIIRIRAGTPFYIVNTDENEKLYIVKLLQPVNLPGHYEVFHGPGGENPESFYRAFSREV 300

Query: 301 LEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNLF 360
           LEAALK PRD+LE+ F++Q  GAI+KAS+EQI+++S+  E  P IWPF+ GK+   FNLF
Sbjct: 301 LEAALKTPRDKLEKLFEKQDEGAIVKASKEQIRAMSRRGE-GPSIWPFT-GKSTGTFNLF 360

Query: 361 KQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGG 420
           K+ P QSN +G        ++  LQ+L I +S+ + T+G M  P YNS+A  I +VV G 
Sbjct: 361 KKDPSQSNNYGQLFESEFKDYPPLQELDIMVSYVNITKGGMSGPFYNSRATKIAIVVSGE 420

Query: 421 GGFQMACPHLGRRGQRGES----SYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVF 480
           G  ++ACPHL      G+     SY+K+   +R D V + PAGHPF   AS    L I+ 
Sbjct: 421 GRLEIACPHLSSSKNSGQEKSGPSYKKLSSSIRTDSVFVVPAGHPFVTVASGNQNLEILC 480

Query: 481 FEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQE 482
           FE+NA+GNI+Y LAGK+NI+  ME+ A+ L F T G+EV ++F  Q+EEFFF GP   Q 
Sbjct: 481 FEVNAEGNIRYTLAGKKNIIEVMEKEAKELAFKTKGEEVDKVFGKQDEEFFFQGPKWRQH 540

BLAST of MC09g0730 vs. ExPASy Swiss-Prot
Match: Q9SEW4 (Vicilin Jug r 2.0101 (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.2e-88
Identity = 187/466 (40.13%), Postives = 288/466 (61.80%), Query Frame = 0

Query: 31  QCRSQCKLKRETAETQKRDCEKRCEETKKKKKKKEKENEGGRTNQISEVEGEETEYEERE 90
           +C+ +C+++ ++ E Q R C++RCE   K+++ +E+  E             E+   E E
Sbjct: 125 RCQRRCQIQEQSPERQ-RQCQQRCERQYKEQQGRERGPEAS--------PRRESRGREEE 184

Query: 91  KEEENPYVFDNEHFKARIETEEGTVRVLPKFTQKSQLLRGIENFRISILEANHSTFVAPS 150
           ++  NPY F ++  ++R E+EEG V+ L +FT++++LLRGIEN+R+ IL+AN +T + P 
Sbjct: 185 QQRHNPYYFHSQSIRSRHESEEGEVKYLERFTERTELLRGIENYRVVILDANPNTSMLPH 244

Query: 151 HFDAEIILFVAQGRATVTVI-RERRASFELQPGDVFRVPSGAPFYLINKDEREKLRIFNL 210
           H DAE +  V +GRAT+T++ +E R SF L+ GDV RVP+GA  Y+IN+D  E+L +  L
Sbjct: 245 HKDAESVAVVTRGRATLTLVSQETRESFNLECGDVIRVPAGATVYVINQDSNERLEMVKL 304

Query: 211 LQATSIPGQLEVFHGPGGEDP-ESFYTAFSWELLEAALKVPRDELERFFKQ--QKGGAIM 270
           LQ  + PGQ   ++  G + P +S+   FS ++L AAL  PRD LERFF Q  Q+ G I+
Sbjct: 305 LQPVNNPGQFREYYAAGAKSPDQSYLRVFSNDILVAALNTPRDRLERFFDQQEQREGVII 364

Query: 271 KASREQIQSLSQHEEIIPRIWPFSEGKTERPFNLFKQSPWQSNKFGHFHIGHPHEFSQLQ 330
           +AS+E++++LSQH     +  P+    +  P +L  +SP  SN+FG F    P E  QLQ
Sbjct: 365 RASQEKLRALSQHAMSAGQ-RPWGRRSSGGPISLKSESPSYSNQFGQFFEACPEEHRQLQ 424

Query: 331 DLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMACPHL--------GRRGQRG 390
           ++ + +++A+   G+MM PHYNSKA  +  VV+G G ++MACPH+        GRR Q  
Sbjct: 425 EMDVLVNYAEIKRGAMMVPHYNSKATVVVYVVEGTGRYEMACPHVSSQSYEGQGRREQEE 484

Query: 391 ESS---YQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINADGNIKYLLAGKEN 450
           E S   +QK+   L R  + + PAGHP +I AS    LR++ F+IN + N +  LAG+ N
Sbjct: 485 EESTGRFQKVTARLARGDIFVIPAGHPIAITASQNENLRLLGFDINGENNQRDFLAGQNN 544

Query: 451 IVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERGQ 482
           I+N++ER A+ L F    +E++ IF  Q E +F   PT+ Q  RGQ
Sbjct: 545 IINQLEREAKELSFNMPREEIEEIFESQMESYFV--PTERQSRRGQ 578

BLAST of MC09g0730 vs. ExPASy Swiss-Prot
Match: B3STU4 (Vicilin Car i 2.0101 OS=Carya illinoinensis OX=32201 GN=pec2a1a PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.6e-88
Identity = 191/467 (40.90%), Postives = 279/467 (59.74%), Query Frame = 0

Query: 31  QCRSQCKLKRETAETQKRDCEKRCEETKKKKKKKEKENEGGRTNQISEVEGEETEYEERE 90
           +C+ +C+ + ++ E Q R C++RCE       ++ KE +G            E+   E E
Sbjct: 321 RCQRRCQTQEQSPERQ-RQCQQRCE-------RQYKEQQGREWGPDQASPRRESRGREEE 380

Query: 91  KEEENPYVFDNEHFKARIETEEGTVRVLPKFTQKSQLLRGIENFRISILEANHSTFVAPS 150
           ++  NPY F ++  ++R E+ EG V+ L +FT++++LLRGIEN+R+ ILEAN +TFV P 
Sbjct: 381 QQRHNPYYFHSQGLRSRHESGEGEVKYLERFTERTELLRGIENYRVVILEANPNTFVLPY 440

Query: 151 HFDAEIILFVAQGRATVT-VIRERRASFELQPGDVFRVPSGAPFYLINKDEREKLRIFNL 210
           H DAE ++ V +GRAT+T V +ERR SF L+ GDV RVP+GA  Y+IN+D  E+L +  L
Sbjct: 441 HKDAESVIVVTRGRATLTFVSQERRESFNLEYGDVIRVPAGATEYVINQDSNERLEMVKL 500

Query: 211 LQATSIPGQLEVFHGPGGEDPESFYTAFSWELLEAALKVPRDELERFFKQ--QKGGAIMK 270
           LQ  + PGQ   ++  G +  ES+   FS ++L AAL  PRD LERFF Q  Q+ G I++
Sbjct: 501 LQPVNNPGQFREYYAAGAQSTESYLRVFSNDILVAALNTPRDRLERFFDQQEQREGVIIR 560

Query: 271 ASREQIQSLSQHEEIIPRIWPFSEGKTERPFNLFKQSPWQSNKFGHFHIGHPHEFSQLQD 330
           AS+E++++LSQH     +  P+    +  P +L  Q    SN+FG F    P E  QLQ+
Sbjct: 561 ASQEKLRALSQHAMSAGQ-RPWGRRSSGGPISLKSQRSSYSNQFGQFFEACPEEHRQLQE 620

Query: 331 LGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMACPH---------LGRRGQRG 390
           + + +++A+   G+MM PHYNSKA  +  VV+G G F+MACPH          GRR Q  
Sbjct: 621 MDVLVNYAEIKRGAMMVPHYNSKATVVVYVVEGTGRFEMACPHDVSSQSYEYKGRREQEE 680

Query: 391 ESS----YQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINADGNIKYLLAGKE 450
           E S    +QK+   L R  + + PAGHP +I AS    LR+V F IN   N +  LAG+ 
Sbjct: 681 EESSTGQFQKVTARLARGDIFVIPAGHPIAITASQNENLRLVGFGINGKNNQRNFLAGQN 740

Query: 451 NIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERGQ 482
           NI+N++ER A+ L F    +E++ IF  Q E +F   P + Q  RGQ
Sbjct: 741 NIINQLEREAKELSFNMPREEIEEIFERQVESYFV--PMERQSRRGQ 776

BLAST of MC09g0730 vs. NCBI nr
Match: XP_022150287.1 (sucrose-binding protein-like isoform X1 [Momordica charantia])

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60
           MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK
Sbjct: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60

Query: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120
           KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK
Sbjct: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120

Query: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180
           FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ
Sbjct: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180

Query: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240
           PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE
Sbjct: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240

Query: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300
           LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL
Sbjct: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300

Query: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360
           FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG
Sbjct: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360

Query: 361 GGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEI 420
           GGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEI
Sbjct: 361 GGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEI 420

Query: 421 NADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERG 480
           NADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERG
Sbjct: 421 NADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERG 480

Query: 481 QHQWADA 487
           QHQWADA
Sbjct: 481 QHQWADA 487

BLAST of MC09g0730 vs. NCBI nr
Match: XP_022150288.1 (sucrose-binding protein-like isoform X2 [Momordica charantia])

HSP 1 Score: 849 bits (2194), Expect = 1.04e-308
Identity = 439/486 (90.33%), Postives = 457/486 (94.03%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60
           MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLK E AE Q+RDCEKRCEETKKK
Sbjct: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKLEIAERQRRDCEKRCEETKKK 60

Query: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120
           KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK
Sbjct: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120

Query: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180
           FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRAT+TVIRERRASFEL+
Sbjct: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATITVIRERRASFELK 180

Query: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240
           PGDVFRVPSGAPFYL NKDEREKLRIF LLQATSIPGQ EVFHGPGG++PESFYTAFSWE
Sbjct: 181 PGDVFRVPSGAPFYLTNKDEREKLRIFKLLQATSIPGQFEVFHGPGGDNPESFYTAFSWE 240

Query: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300
           LLEAALKVPRDELERFFKQQKGGAIM+ASREQIQSLSQ EEIIPRIWPFSEGKTERPFNL
Sbjct: 241 LLEAALKVPRDELERFFKQQKGGAIMRASREQIQSLSQQEEIIPRIWPFSEGKTERPFNL 300

Query: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360
           FKQSPWQSNKFGHF I HPHEFSQLQDLGIA+SFA+FTEGSMMAPHYNSKA+ +GVVVDG
Sbjct: 301 FKQSPWQSNKFGHFFIAHPHEFSQLQDLGIAVSFANFTEGSMMAPHYNSKAIKVGVVVDG 360

Query: 361 GGGFQMACPHLGRR---GQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVF 420
            G FQMACPH GR    G RGESSYQ+IRGVLRRD+VIIAPAGHPFSI A+P H L+IVF
Sbjct: 361 EGEFQMACPHRGRHHGGGHRGESSYQRIRGVLRRDMVIIAPAGHPFSIIAAPNHRLQIVF 420

Query: 421 FEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQE 480
           FEINADGNIKYLLAGKENI+NKMERIAR LGFGT+GK V R+FR QEEEFFFPGPTQ   
Sbjct: 421 FEINADGNIKYLLAGKENIINKMERIARELGFGTSGKAVGRMFRQQEEEFFFPGPTQ--- 480

Query: 481 ERGQHQ 483
           + GQHQ
Sbjct: 481 QHGQHQ 483

BLAST of MC09g0730 vs. NCBI nr
Match: XP_008451293.1 (PREDICTED: sucrose-binding protein-like [Cucumis melo])

HSP 1 Score: 629 bits (1621), Expect = 4.76e-221
Identity = 328/503 (65.21%), Postives = 393/503 (78.13%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVL----AGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEE 60
           MA KIK++L L LLL+  +  L    A KDP+LKQC+ QCK++R+  E QKRDCE+ C+E
Sbjct: 26  MAFKIKINLNLHLLLLFLITALCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDE 85

Query: 61  TKKKKKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVR 120
             K KK+K      GR  +  E E EE E  E E+EEENPYVFD+EHF  ++ET EG ++
Sbjct: 86  YYKMKKEK------GRNYESEEEEEEEEE--EEEEEEENPYVFDDEHFVGKVETGEGKIK 145

Query: 121 VLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRAS 180
           VL KFTQ+SQLLRGIENFR+SI+EAN STFV P+HFDAEII+FVAQGR T+TVI+E+R S
Sbjct: 146 VLQKFTQRSQLLRGIENFRVSIVEANPSTFVVPTHFDAEIIMFVAQGRGTITVIKEKRGS 205

Query: 181 FELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTA 240
           F+L+ GDVFR+PSGAPFY INKDE +KL+I  LLQ+TS+PG  + F   GGE+PESFYTA
Sbjct: 206 FDLKCGDVFRIPSGAPFYFINKDEHQKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTA 265

Query: 241 FSWELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTER 300
           FSW+LLEAA K+PRD+LERFFKQQ+ G I+KASREQI+SLSQHEEIIP+IWPFSEG+TER
Sbjct: 266 FSWDLLEAAFKIPRDKLERFFKQQRPGTIIKASREQIRSLSQHEEIIPKIWPFSEGETER 325

Query: 301 PFNLFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGV 360
           PFNL KQ PWQSNKFG     +P EFSQL+DLG+AI+FA+ T+GSMM PHYNSK+M I V
Sbjct: 326 PFNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANITKGSMMTPHYNSKSMKIAV 385

Query: 361 VVDGGGGFQMACPHL-------GR-----RGQRGESSYQKIRGVLRRDVVIIAPAGHPFS 420
           V+DG GGFQMACPHL       GR      G++GE +YQKIRG L R VV + PAGHPFS
Sbjct: 386 VLDGEGGFQMACPHLSSSSGRSGRWSEREEGRKGERTYQKIRGRLSRGVVFVVPAGHPFS 445

Query: 421 IFASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQE 480
           +FASP H L+IV FE+NA GN KY LAGKENIVNKME IAR LGF T G+EV+R+F+ QE
Sbjct: 446 VFASPNHSLQIVCFEVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQE 505

Query: 481 EEFFFPGPTQLQEERGQHQWADA 487
           EEFFFPGP Q +     H+WA A
Sbjct: 506 EEFFFPGPNQQE-----HEWASA 515

BLAST of MC09g0730 vs. NCBI nr
Match: XP_038898840.1 (vicilin Jug r 6.0101-like [Benincasa hispida])

HSP 1 Score: 627 bits (1616), Expect = 9.00e-221
Identity = 325/499 (65.13%), Postives = 395/499 (79.16%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60
           MA K KL L LL LL L+ A LA KDP+LKQC+ QCK++R+  E Q+RDCE+ C+E  K 
Sbjct: 1   MASKFKLPL-LLFLLFLTAACLASKDPELKQCKHQCKVQRQFDEQQRRDCERSCDEYYKM 60

Query: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120
           KK++E+   G   N   E EG++ +      EE+NPYVF+++HF+ ++ET EG +RVL K
Sbjct: 61  KKERER---GKGRNYEREEEGDDDD------EEKNPYVFEDKHFEGKVETGEGRIRVLQK 120

Query: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180
           FTQ+S+LLRGIENFR+SI+EAN STFV P+HFDAEI+LFVAQGR T+TVI+E+R SF+++
Sbjct: 121 FTQRSELLRGIENFRVSIVEANPSTFVIPTHFDAEILLFVAQGRGTITVIKEKRGSFDVK 180

Query: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240
            GDVFR+PSGAPFY INKDE +KL+I  LLQ TS+PG  E+F   GGE+ ESFYTAFSW+
Sbjct: 181 CGDVFRIPSGAPFYFINKDEHQKLKIVKLLQPTSVPGHFEIFQPAGGENSESFYTAFSWD 240

Query: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300
           LLEAA K+PR++LERFF QQK G I+KASREQI+SLSQHEEIIPRIWPFSEG+TERPFNL
Sbjct: 241 LLEAAFKIPREKLERFFDQQKPGTIIKASREQIRSLSQHEEIIPRIWPFSEGETERPFNL 300

Query: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360
            KQ PWQSNKFG     +P EFSQL+DLG+A++FA+ T+GSMMAPHYNSKAM I VVV+G
Sbjct: 301 LKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAVAFANMTKGSMMAPHYNSKAMKIAVVVEG 360

Query: 361 GGGFQMACPHL----GRRGQ--------RGESSYQKIRGVLRRDVVIIAPAGHPFSIFAS 420
            GGFQMACPHL    GR G+        +G  +YQKIRG LRR VV I PAGHPFS+FAS
Sbjct: 361 EGGFQMACPHLSSSSGRSGRWSEREQERKGGRTYQKIRGQLRRGVVFIVPAGHPFSVFAS 420

Query: 421 PGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFF 480
           P HGL+IV FE+NA GN KYLLAGK+NIVNKMER+AR LGF T G+EV+R+FR QEEEFF
Sbjct: 421 PNHGLQIVCFEVNAYGNTKYLLAGKDNIVNKMERVARELGFNTPGREVERMFRQQEEEFF 480

Query: 481 FPGPTQLQEERGQHQWADA 487
           FPGP Q +     H+WA+A
Sbjct: 481 FPGPNQQE-----HEWAEA 484

BLAST of MC09g0730 vs. NCBI nr
Match: XP_011659275.1 (sucrose-binding protein [Cucumis sativus] >KAE8646304.1 hypothetical protein Csa_016372 [Cucumis sativus])

HSP 1 Score: 619 bits (1595), Expect = 3.54e-217
Identity = 331/503 (65.81%), Postives = 388/503 (77.14%), Query Frame = 0

Query: 1   MAPKIKLSLPL-LLLLVLSVAV---LAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEE 60
           MA KIK++L L LLLL L  AV   LA KDP+LKQC+ QCK++R+  E QKRDCE+ C+E
Sbjct: 26  MAFKIKINLNLHLLLLFLITAVCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDE 85

Query: 61  TKKKKKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVR 120
             K KK+K      GR  +  E E EE E E       NPYVFD+EHF  +IET EG ++
Sbjct: 86  YYKMKKEK------GRNYESEEEEEEEEEVE-------NPYVFDDEHFVGQIETGEGKIK 145

Query: 121 VLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRAS 180
           VL KFTQ+S LLRGIENFR+SI+EAN STFV P+HFDAEIILFVAQGR T+TVI+E+R S
Sbjct: 146 VLQKFTQRSHLLRGIENFRVSIVEANPSTFVVPTHFDAEIILFVAQGRGTITVIKEKRGS 205

Query: 181 FELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTA 240
           FEL+ GDVFR+PSGAPFY INKDE  KL+I  LLQ+TS+PG  + F   GGE+PESFYTA
Sbjct: 206 FELKCGDVFRIPSGAPFYFINKDEHRKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTA 265

Query: 241 FSWELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTER 300
           FSW+LLEAA K+PRD+LERFFKQQK G I+KASREQI+SLS+HEEIIP+IWPFSEG+TER
Sbjct: 266 FSWDLLEAAFKIPRDKLERFFKQQKPGTIIKASREQIRSLSRHEEIIPKIWPFSEGETER 325

Query: 301 PFNLFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGV 360
           PFNL KQ P QSNKFG     +P EFSQL+DLG+AI+FA+ TEGSM+APHYNSK+M I V
Sbjct: 326 PFNLLKQHPCQSNKFGRLFEAYPDEFSQLRDLGVAIAFANITEGSMVAPHYNSKSMKIAV 385

Query: 361 VVDGGGGFQMACPHL-------GRRGQR-----GESSYQKIRGVLRRDVVIIAPAGHPFS 420
           V+DG GGFQMACPHL       GR  +R     GE +YQKIRG L R VV + PAGHPFS
Sbjct: 386 VLDGQGGFQMACPHLSSSSRRSGRWSEREEERKGERTYQKIRGRLSRGVVFVVPAGHPFS 445

Query: 421 IFASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQE 480
           +FASP H L+IV FE+NA GN KY LAGKENIVNKME IAR LGF T G+EV+R+F+ QE
Sbjct: 446 VFASPNHSLQIVCFEVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQE 505

Query: 481 EEFFFPGPTQLQEERGQHQWADA 487
           EEFFFPGP Q +     H+WADA
Sbjct: 506 EEFFFPGPNQQE-----HEWADA 510

BLAST of MC09g0730 vs. ExPASy TrEMBL
Match: A0A6J1DAC2 (sucrose-binding protein-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018489 PE=4 SV=1)

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60
           MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK
Sbjct: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60

Query: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120
           KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK
Sbjct: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120

Query: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180
           FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ
Sbjct: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180

Query: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240
           PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE
Sbjct: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240

Query: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300
           LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL
Sbjct: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300

Query: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360
           FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG
Sbjct: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360

Query: 361 GGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEI 420
           GGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEI
Sbjct: 361 GGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEI 420

Query: 421 NADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERG 480
           NADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERG
Sbjct: 421 NADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERG 480

Query: 481 QHQWADA 487
           QHQWADA
Sbjct: 481 QHQWADA 487

BLAST of MC09g0730 vs. ExPASy TrEMBL
Match: A0A6J1D921 (sucrose-binding protein-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018489 PE=4 SV=1)

HSP 1 Score: 849 bits (2194), Expect = 5.02e-309
Identity = 439/486 (90.33%), Postives = 457/486 (94.03%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60
           MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLK E AE Q+RDCEKRCEETKKK
Sbjct: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKLEIAERQRRDCEKRCEETKKK 60

Query: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120
           KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK
Sbjct: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVRVLPK 120

Query: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFELQ 180
           FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRAT+TVIRERRASFEL+
Sbjct: 121 FTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATITVIRERRASFELK 180

Query: 181 PGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFSWE 240
           PGDVFRVPSGAPFYL NKDEREKLRIF LLQATSIPGQ EVFHGPGG++PESFYTAFSWE
Sbjct: 181 PGDVFRVPSGAPFYLTNKDEREKLRIFKLLQATSIPGQFEVFHGPGGDNPESFYTAFSWE 240

Query: 241 LLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPFNL 300
           LLEAALKVPRDELERFFKQQKGGAIM+ASREQIQSLSQ EEIIPRIWPFSEGKTERPFNL
Sbjct: 241 LLEAALKVPRDELERFFKQQKGGAIMRASREQIQSLSQQEEIIPRIWPFSEGKTERPFNL 300

Query: 301 FKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDG 360
           FKQSPWQSNKFGHF I HPHEFSQLQDLGIA+SFA+FTEGSMMAPHYNSKA+ +GVVVDG
Sbjct: 301 FKQSPWQSNKFGHFFIAHPHEFSQLQDLGIAVSFANFTEGSMMAPHYNSKAIKVGVVVDG 360

Query: 361 GGGFQMACPHLGRR---GQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVF 420
            G FQMACPH GR    G RGESSYQ+IRGVLRRD+VIIAPAGHPFSI A+P H L+IVF
Sbjct: 361 EGEFQMACPHRGRHHGGGHRGESSYQRIRGVLRRDMVIIAPAGHPFSIIAAPNHRLQIVF 420

Query: 421 FEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQE 480
           FEINADGNIKYLLAGKENI+NKMERIAR LGFGT+GK V R+FR QEEEFFFPGPTQ   
Sbjct: 421 FEINADGNIKYLLAGKENIINKMERIARELGFGTSGKAVGRMFRQQEEEFFFPGPTQ--- 480

Query: 481 ERGQHQ 483
           + GQHQ
Sbjct: 481 QHGQHQ 483

BLAST of MC09g0730 vs. ExPASy TrEMBL
Match: A0A1S3BS82 (sucrose-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103492635 PE=3 SV=1)

HSP 1 Score: 629 bits (1621), Expect = 2.30e-221
Identity = 328/503 (65.21%), Postives = 393/503 (78.13%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVL----AGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEE 60
           MA KIK++L L LLL+  +  L    A KDP+LKQC+ QCK++R+  E QKRDCE+ C+E
Sbjct: 26  MAFKIKINLNLHLLLLFLITALCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDE 85

Query: 61  TKKKKKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVR 120
             K KK+K      GR  +  E E EE E  E E+EEENPYVFD+EHF  ++ET EG ++
Sbjct: 86  YYKMKKEK------GRNYESEEEEEEEEE--EEEEEEENPYVFDDEHFVGKVETGEGKIK 145

Query: 121 VLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRAS 180
           VL KFTQ+SQLLRGIENFR+SI+EAN STFV P+HFDAEII+FVAQGR T+TVI+E+R S
Sbjct: 146 VLQKFTQRSQLLRGIENFRVSIVEANPSTFVVPTHFDAEIIMFVAQGRGTITVIKEKRGS 205

Query: 181 FELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTA 240
           F+L+ GDVFR+PSGAPFY INKDE +KL+I  LLQ+TS+PG  + F   GGE+PESFYTA
Sbjct: 206 FDLKCGDVFRIPSGAPFYFINKDEHQKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTA 265

Query: 241 FSWELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTER 300
           FSW+LLEAA K+PRD+LERFFKQQ+ G I+KASREQI+SLSQHEEIIP+IWPFSEG+TER
Sbjct: 266 FSWDLLEAAFKIPRDKLERFFKQQRPGTIIKASREQIRSLSQHEEIIPKIWPFSEGETER 325

Query: 301 PFNLFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGV 360
           PFNL KQ PWQSNKFG     +P EFSQL+DLG+AI+FA+ T+GSMM PHYNSK+M I V
Sbjct: 326 PFNLLKQHPWQSNKFGRLFEAYPDEFSQLRDLGVAIAFANITKGSMMTPHYNSKSMKIAV 385

Query: 361 VVDGGGGFQMACPHL-------GR-----RGQRGESSYQKIRGVLRRDVVIIAPAGHPFS 420
           V+DG GGFQMACPHL       GR      G++GE +YQKIRG L R VV + PAGHPFS
Sbjct: 386 VLDGEGGFQMACPHLSSSSGRSGRWSEREEGRKGERTYQKIRGRLSRGVVFVVPAGHPFS 445

Query: 421 IFASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQE 480
           +FASP H L+IV FE+NA GN KY LAGKENIVNKME IAR LGF T G+EV+R+F+ QE
Sbjct: 446 VFASPNHSLQIVCFEVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQE 505

Query: 481 EEFFFPGPTQLQEERGQHQWADA 487
           EEFFFPGP Q +     H+WA A
Sbjct: 506 EEFFFPGPNQQE-----HEWASA 515

BLAST of MC09g0730 vs. ExPASy TrEMBL
Match: A0A0A0KAE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G380130 PE=4 SV=1)

HSP 1 Score: 619 bits (1595), Expect = 7.01e-218
Identity = 331/503 (65.81%), Postives = 388/503 (77.14%), Query Frame = 0

Query: 1   MAPKIKLSLPL-LLLLVLSVAV---LAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEE 60
           MA KIK++L L LLLL L  AV   LA KDP+LKQC+ QCK++R+  E QKRDCE+ C+E
Sbjct: 1   MAFKIKINLNLHLLLLFLITAVCLALASKDPELKQCKHQCKVQRQFDEQQKRDCERSCDE 60

Query: 61  TKKKKKKKEKENEGGRTNQISEVEGEETEYEEREKEEENPYVFDNEHFKARIETEEGTVR 120
             K KK+K      GR  +  E E EE E E       NPYVFD+EHF  +IET EG ++
Sbjct: 61  YYKMKKEK------GRNYESEEEEEEEEEVE-------NPYVFDDEHFVGQIETGEGKIK 120

Query: 121 VLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRAS 180
           VL KFTQ+S LLRGIENFR+SI+EAN STFV P+HFDAEIILFVAQGR T+TVI+E+R S
Sbjct: 121 VLQKFTQRSHLLRGIENFRVSIVEANPSTFVVPTHFDAEIILFVAQGRGTITVIKEKRGS 180

Query: 181 FELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTA 240
           FEL+ GDVFR+PSGAPFY INKDE  KL+I  LLQ+TS+PG  + F   GGE+PESFYTA
Sbjct: 181 FELKCGDVFRIPSGAPFYFINKDEHRKLKIVKLLQSTSVPGHFQTFQPAGGENPESFYTA 240

Query: 241 FSWELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTER 300
           FSW+LLEAA K+PRD+LERFFKQQK G I+KASREQI+SLS+HEEIIP+IWPFSEG+TER
Sbjct: 241 FSWDLLEAAFKIPRDKLERFFKQQKPGTIIKASREQIRSLSRHEEIIPKIWPFSEGETER 300

Query: 301 PFNLFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGV 360
           PFNL KQ P QSNKFG     +P EFSQL+DLG+AI+FA+ TEGSM+APHYNSK+M I V
Sbjct: 301 PFNLLKQHPCQSNKFGRLFEAYPDEFSQLRDLGVAIAFANITEGSMVAPHYNSKSMKIAV 360

Query: 361 VVDGGGGFQMACPHL-------GRRGQR-----GESSYQKIRGVLRRDVVIIAPAGHPFS 420
           V+DG GGFQMACPHL       GR  +R     GE +YQKIRG L R VV + PAGHPFS
Sbjct: 361 VLDGQGGFQMACPHLSSSSRRSGRWSEREEERKGERTYQKIRGRLSRGVVFVVPAGHPFS 420

Query: 421 IFASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQE 480
           +FASP H L+IV FE+NA GN KY LAGKENIVNKME IAR LGF T G+EV+R+F+ QE
Sbjct: 421 VFASPNHSLQIVCFEVNAYGNTKYFLAGKENIVNKMESIARELGFNTPGREVERMFKQQE 480

Query: 481 EEFFFPGPTQLQEERGQHQWADA 487
           EEFFFPGP Q +     H+WADA
Sbjct: 481 EEFFFPGPNQQE-----HEWADA 485

BLAST of MC09g0730 vs. ExPASy TrEMBL
Match: A0A6J1JS85 (sucrose-binding protein-like OS=Cucurbita maxima OX=3661 GN=LOC111488449 PE=4 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 3.93e-209
Identity = 318/501 (63.47%), Postives = 385/501 (76.85%), Query Frame = 0

Query: 1   MAPKIKLSLPLLLLLVLSVAVLAGKDPDLKQCRSQCKLKRETAETQKRDCEKRCEETKKK 60
           MA KI L   LLLLL ++V  LA KDP+L+QC+ QC+++++  E QKRDCEK C+E  K 
Sbjct: 1   MASKIYL---LLLLLSVTVGCLAFKDPELEQCKHQCRVQQKFDEKQKRDCEKMCDEYHKM 60

Query: 61  KKKKEKENEGGRTNQISEVEGEETEYEEREKE--EENPYVFDNEHFKARIETEEGTVRVL 120
           KK  E+E E GR  +  E         ERE+E  E NPYVFD+EHF+ R+ET EG +RVL
Sbjct: 61  KK--EREREEGRYYESEE---------EREEEYGESNPYVFDDEHFEERVETGEGRIRVL 120

Query: 121 PKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATVTVIRERRASFE 180
            +FTQ+S+LLRGIEN+R+S++E N STFV+PSHFDAEIILFVA+GR T+TVI+E+R SF+
Sbjct: 121 QRFTQRSELLRGIENYRVSLVEVNPSTFVSPSHFDAEIILFVAEGRGTITVIKEKRQSFD 180

Query: 181 LQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPGGEDPESFYTAFS 240
           ++ GDVFRVPSGAPFY INKDE +KL+I  LLQ+TS+PG  E+F  PGGE+PESFYTAFS
Sbjct: 181 VKCGDVFRVPSGAPFYFINKDEHQKLKIVKLLQSTSVPGSFEIFQ-PGGENPESFYTAFS 240

Query: 241 WELLEAALKVPRDELERFFKQQKGGAIMKASREQIQSLSQHEEIIPRIWPFSEGKTERPF 300
           W+LLEAA K+P+D+L+RFF+QQK G I+KASREQI+SLS+ EE IPRIWPFSEG+TERPF
Sbjct: 241 WDLLEAAFKIPKDKLKRFFEQQKEGTIIKASREQIRSLSRPEEFIPRIWPFSEGETERPF 300

Query: 301 NLFKQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVV 360
           NL KQ PWQSNKFG      P EF QL+DL +A++FA+ T+GSMM PHY SKA  I VVV
Sbjct: 301 NLLKQDPWQSNKFGRLFEADPGEFRQLRDLNVAVAFANMTKGSMMTPHYYSKATKIAVVV 360

Query: 361 DGGGGFQMACPHL-------GRRGQR-----GESSYQKIRGVLRRDVVIIAPAGHPFSIF 420
           DG GGFQMACPHL       GR  +R     GE +YQKIRG LRR VV I PAGHPFS+F
Sbjct: 361 DGEGGFQMACPHLSSSSGRGGRWSEREHKRTGEGTYQKIRGRLRRGVVFIIPAGHPFSVF 420

Query: 421 ASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEE 480
           ASP H L+IV FE+NA GN KYLLAGK+NIVNKME +AR LGF   G+EV+R+FR QEEE
Sbjct: 421 ASPNHNLQIVCFEVNAYGNTKYLLAGKDNIVNKMESVARELGFNIPGREVERMFRQQEEE 480

Query: 481 FFFPGPTQLQEERGQHQWADA 487
           FF PGP Q +     H+W DA
Sbjct: 481 FFLPGPDQQE-----HEWTDA 481

BLAST of MC09g0730 vs. TAIR 10
Match: AT3G22640.1 (cupin family protein )

HSP 1 Score: 226.1 bits (575), Expect = 6.1e-59
Identity = 136/420 (32.38%), Postives = 218/420 (51.90%), Query Frame = 0

Query: 90  EKEEENPYVFDNEHFKARIETEEGTVRVLPKFTQKS-QLLRGIENFRISILEANHSTFVA 149
           E+   +PY F    F    +++EG VRVLPKFT+ +  L RGIEN+R S++E   +TF  
Sbjct: 57  EESTNHPYHFRKRSFSDWFQSKEGFVRVLPKFTKHAPALFRGIENYRFSLVEMEPTTFFV 116

Query: 150 PSHFDAEIILFVAQGRATVTVIRER-RASFELQPGDVFRVPSGAPFYLINKDEREKLRIF 209
           P H DA+ +  V QG+  +  + ++ + SF +  GDV R+PSG   ++ N ++   LR+ 
Sbjct: 117 PHHLDADAVFIVLQGKGVIEFVTDKTKESFHITKGDVVRIPSGVTNFITNTNQTVPLRLA 176

Query: 210 NLLQATSIPGQLEVFHGPGGEDPESFYTAFSWELLEAALKVPRDELERFFKQQK---GGA 269
            +    + PG  + +     +  +S++  F+ E+L  +  VP + L R   + K    G 
Sbjct: 177 QITVPVNNPGNYKDYFPAASQFQQSYFNGFTKEVLSTSFNVPEELLGRLVTRSKEIGQGI 236

Query: 270 IMKASREQIQSLSQHEEIIPRIWPFSEGKTE--------RPFNLFKQSPWQSNKFGHFHI 329
           I + S +QI+ L++H           + K E         PFNLF   P  SN FGHFH 
Sbjct: 237 IRRISPDQIKELAEHATSPSNKHKAKKEKEEDKDLRTLWTPFNLFAIDPIYSNDFGHFHE 296

Query: 330 GHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMACPHLGRRGQ 389
            HP  ++QLQDL IA ++A+ T+GS+  PH+NSK   +  V +G   F+MA P+  +RGQ
Sbjct: 297 AHPKNYNQLQDLHIAAAWANMTQGSLFLPHFNSKTTFVTFVENGCARFEMATPYKFQRGQ 356

Query: 390 R-------------GESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINAD 449
           +              E+ ++ +  V + +V I+ PAGHPF+I  S       V F I A 
Sbjct: 357 QQWPGQGQEEEEDMSENVHKVVSRVCKGEVFIV-PAGHPFTIL-SQDQDFIAVGFGIYAT 416

Query: 450 GNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQLQEERGQHQ 484
            + +  LAG+EN+++ +   A  + FG   K  +++F  Q   +F P     Q+   +H+
Sbjct: 417 NSKRTFLAGEENLLSNLNPAATRVTFGVGSKVAEKLFTSQNYSYFAPTSRSQQQIPEKHK 474

BLAST of MC09g0730 vs. TAIR 10
Match: AT2G28490.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 3.9e-21
Identity = 97/426 (22.77%), Postives = 169/426 (39.67%), Query Frame = 0

Query: 108 IETEEGTVRVLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATV 167
           I++E G +RV+   + + +++   +   I  L     T   P + D+ +++F+ QG AT+
Sbjct: 95  IKSEGGEMRVV--LSPRGRIIE--KPMHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATL 154

Query: 168 TVI-RERRASFELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPG--QLEVFHG 227
            VI ++     +L+ GD++ +P+G+ FYL N    ++L +   +  T   G    + F+ 
Sbjct: 155 GVICKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQRLHVICSIDPTQSLGFETFQPFYI 214

Query: 228 PGGEDPESFYTAFSWELLEAALKVPRDELERFFKQQKGGAIM------------------ 287
            GG  P S    F    L +A  V   EL++    Q  G I+                  
Sbjct: 215 GGG--PSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQPQPQSTVWTQF 274

Query: 288 -----KASREQIQSLSQHEEIIPRIWPFSEG------------------------KTERP 347
                +   +Q++ L + ++  P+   +S G                        + E  
Sbjct: 275 LGLRGEEKHKQLKKLLETKQGSPQDQQYSSGWSWRNIVRSILDLTEEKNKGSGSSECEDS 334

Query: 348 FNLF--KQSPWQSNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIG 407
           +N++  K  P   NK+G        ++  L+  GI +   + T G+MMAPH N  A   G
Sbjct: 335 YNIYDKKDKPSFDNKYGWSIALDYDDYKPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYG 394

Query: 408 VVVDGGGGFQMACPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRI 467
           +V+ G G  Q+  P+       G S+      V   DV  I P    F   AS       
Sbjct: 395 IVLAGSGEIQVVFPN-------GTSAMNTRVSV--GDVFWI-PRYFAFCQIASRTGPFEF 454

Query: 468 VFFEINADGNIKYLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEEEFFFPGPTQL 482
           V F  +A  N    L G  +++  +   +  + FG   + ++R    Q E    P P   
Sbjct: 455 VGFTTSAHKNRPQFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPAAA 504

BLAST of MC09g0730 vs. TAIR 10
Match: AT2G18540.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 90.5 bits (223), Expect = 4.0e-18
Identity = 83/385 (21.56%), Postives = 157/385 (40.78%), Query Frame = 0

Query: 108 IETEEGTVRVLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDAEIILFVAQGRATV 167
           ++ ++ T  V  +F   S +  G + + I  +    +  + P    ++++ FV  G   +
Sbjct: 45  VKKDQRTSVVATEFGNISAVQIG-DGYHIQFITLEPNALLLPLLLHSDMVFFVHTGTGIL 104

Query: 168 TVI-RERRASFELQPGDVFRVPSGAPFYLINKDEREKLRIFNLLQATSIPGQLEVFHGPG 227
             I  E     EL+ GDVFR+ SG  FY+ + ++     IFN+ +  + P  L  +    
Sbjct: 105 NWIDEESERKLELRRGDVFRLRSGTVFYVHSNEKLRVYAIFNVGKCLNDP-CLGAY---- 164

Query: 228 GEDPESFYTAFSWELLEAALKVPRDELERFFKQQKGGAIMKA-SREQIQSLSQH--EEII 287
                     F    L +A  VP D L +     K   I+ A  R + Q L +   +  +
Sbjct: 165 -SSVRDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIVNALPRNRTQGLEEDKWQSRL 224

Query: 288 PRIWPFSEGKTE---------------RPFNLFKQSPWQSNKFGHFHIGHPHEFSQLQDL 347
            R++   E  T+               R FN+F++ P   N  G   +    +   L+  
Sbjct: 225 VRLFVSVEDVTDHLAMKPIVDTNKKKSRTFNVFEEDPDFENNNGRSIVVDEKDLDALKGS 284

Query: 348 GIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMACPHLGRRGQRGESSYQKIRG- 407
              +   + T+GSM+ PH+N  A  I +V++G G  ++         Q+  SS +  R  
Sbjct: 285 RFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVV-------NQQSLSSCKNDRKS 344

Query: 408 ---VLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINADGNIKYLLAGKENIVNKMERIA 467
              ++    V + P  HP +  +        + F  +A  N    L G+ +++  ++R  
Sbjct: 345 ESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFLVGQSSVLKVLDRDV 404

Query: 468 RGLGFGTAGKEVKRIFRHQEEEFFF 470
             + F  + + +K + + Q+E   F
Sbjct: 405 VAVSFNLSNETIKGLLKAQKESVIF 415

BLAST of MC09g0730 vs. TAIR 10
Match: AT4G36700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 8.4e-16
Identity = 78/398 (19.60%), Postives = 154/398 (38.69%), Query Frame = 0

Query: 95  NPYVFDNEHFKARIETEEGTVRVLPKFTQKSQLLRGIENFRISILEANHSTFVAPSHFDA 154
           +P +   + +K   ET+ G +  +    Q      G+  ++I  +    +T + P    +
Sbjct: 45  SPLLIKKDQWKPIFETKFGQISTV----QIGNGCGGMGPYKIHSITLEPNTILLPLLLHS 104

Query: 155 EIILFVAQGRATVTVIRERRASFELQPGDVFRVPSGAPFYLINKDE----REKLRIFNLL 214
           +++ FV  G   +  + E   S E++ GDV+R+  G+ FYL +K        KL+++ + 
Sbjct: 105 DMVFFVDSGSGILNWVDEEAKSTEIRLGDVYRLRPGSVFYLQSKPVDIFLGTKLKLYAIF 164

Query: 215 QATSIPGQLEVFHGP---GGEDPESFYTAFSWELLEAALKVPRDELERFFKQQKGGAIMK 274
                    E  H P              F   +L++A  VP   +E    + K   I+ 
Sbjct: 165 SNND-----ECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEGIIELMRNRTKPPLIVS 224

Query: 275 ASREQIQSLSQHEEIIPRIWPFSEG--------------------KTERPFNLFKQSPWQ 334
            +      ++   ++ PR+     G                    K  + FN+F+  P  
Sbjct: 225 ETL-CTPGVANTWQLQPRLLKLFAGSADLVDNKKKKEKKEKKEKVKKAKTFNVFESEPDF 284

Query: 335 SNKFGHFHIGHPHEFSQLQDLGIAISFADFTEGSMMAPHYNSKAMTIGVVVDGGGGFQMA 394
            + +G     +  +   L+   + +S  + T+GSMM PH+N  A  I +V+ G G  ++ 
Sbjct: 285 ESPYGRTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKGAGMVRVL 344

Query: 395 CPHLGRRGQRGESSYQKIRGVLRRDVVIIAPAGHPFSIFASPGHGLRIVFFEINADGNIK 454
              +        S  + +R  +    +   P  HP +  +     L  V F  +A  N  
Sbjct: 345 RSSI---SSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEP 404

Query: 455 YLLAGKENIVNKMERIARGLGFGTAGKEVKRIFRHQEE 466
             LAG+++ +  ++R         +   +  +   Q+E
Sbjct: 405 QFLAGEDSALRMLDRQVLAASLNVSSVTIDGLLGAQKE 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A2I4E5L63.8e-13851.21Vicilin Jug r 6.0101 OS=Juglans regia OX=51240 GN=LOC108986502 PE=1 SV=1[more]
Q8S4P98.1e-13352.38Vicilin Cor a 11.0101 OS=Corylus avellana OX=13451 PE=1 SV=1[more]
B4X6404.3e-11844.49Vicilin Pis v 3.0101 OS=Pistacia vera OX=55513 PE=1 SV=2[more]
Q9SEW41.2e-8840.13Vicilin Jug r 2.0101 (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1[more]
B3STU41.6e-8840.90Vicilin Car i 2.0101 OS=Carya illinoinensis OX=32201 GN=pec2a1a PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022150287.10.0100.00sucrose-binding protein-like isoform X1 [Momordica charantia][more]
XP_022150288.11.04e-30890.33sucrose-binding protein-like isoform X2 [Momordica charantia][more]
XP_008451293.14.76e-22165.21PREDICTED: sucrose-binding protein-like [Cucumis melo][more]
XP_038898840.19.00e-22165.13vicilin Jug r 6.0101-like [Benincasa hispida][more]
XP_011659275.13.54e-21765.81sucrose-binding protein [Cucumis sativus] >KAE8646304.1 hypothetical protein Csa... [more]
Match NameE-valueIdentityDescription
A0A6J1DAC20.0100.00sucrose-binding protein-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1D9215.02e-30990.33sucrose-binding protein-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3BS822.30e-22165.21sucrose-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103492635 PE=3 SV=1[more]
A0A0A0KAE27.01e-21865.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G380130 PE=4 SV=1[more]
A0A6J1JS853.93e-20963.47sucrose-binding protein-like OS=Cucurbita maxima OX=3661 GN=LOC111488449 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT3G22640.16.1e-5932.38cupin family protein [more]
AT2G28490.13.9e-2122.77RmlC-like cupins superfamily protein [more]
AT2G18540.14.0e-1821.56RmlC-like cupins superfamily protein [more]
AT4G36700.18.4e-1619.60RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 40..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..79
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 4..480
NoneNo IPR availablePANTHERPTHR31189:SF1363 KDA GLOBULIN-LIKE PROTEINcoord: 4..480
NoneNo IPR availableCDDcd02245cupin_7S_vicilin-like_Ccoord: 305..466
e-value: 2.2404E-57
score: 185.798
NoneNo IPR availableCDDcd02244cupin_7S_vicilin-like_Ncoord: 107..281
e-value: 1.25425E-80
score: 246.266
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 102..254
e-value: 1.0E-4
score: 22.1
coord: 298..457
e-value: 2.8E-38
score: 143.2
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 298..455
e-value: 3.7E-22
score: 78.5
coord: 138..206
e-value: 1.8E-7
score: 30.9
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 289..473
e-value: 2.8E-50
score: 172.3
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 86..277
e-value: 6.8E-73
score: 246.2
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 266..470
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 95..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0730.1MC09g0730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034214 protein hexamerization
biological_process GO:0070207 protein homotrimerization
biological_process GO:0010431 seed maturation
cellular_component GO:0043245 extraorganismal space
molecular_function GO:0005507 copper ion binding
molecular_function GO:0045735 nutrient reservoir activity