MC09g0696 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0696
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionBifunctional inhibitor/plant lipid transfer protein/seed storage helical domain containing protein
LocationMC09: 6447098 .. 6447615 (+)
RNA-Seq ExpressionMC09g0696
SyntenyMC09g0696
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGTCCGGGATCCTCGTACTGGTATTAGCGCTGTTCTTGGTGCTGGTTACCAACACAGAGAGTCAATGGCAGCCGCCAGAGGTGCCAGAGGTTGCTCCCCCTCCGCTCTGCGCCTCCCAGATAGCGCTAGTAAACTATGCCTGCGGAATGCTGCCTTACACCCCAGTGCTGCCAACTCCGCCTTCACCACCATCACTCTTTGATAACCATGAAACCCAATGCCCTCAGCCCAGCATCCGCCCCCGCCCCCACCCCCGCCCTCGCCCCNCCCCCACCCCCGCCCTCGCCCCCACCACAGGCACCGGCACAGACACGAGCAGAGTCACCAGCTCAAGCTCCCGATGTCACCAATGGAGGAGAATTGTTGCAAGTGGGTGCAGCAGTTGGATAGTGTATGCGTATGCGGGCTGCTATCCCGCTTGCCCGCCTTCCTCGAGAGGCCTCAGCATAACTTTTCTATTACTGTTGGCGGTTCTTGTGATGCTACATACTCATGTGGAGGAGGGATCAAA

mRNA sequence

ATGGCGAGGTCCGGGATCCTCGTACTGGTATTAGCGCTGTTCTTGGTGCTGGTTACCAACACAGAGAGTCAATGGCAGCCGCCAGAGGTGCCAGAGGTTGCTCCCCCTCCGCTCTGCGCCTCCCAGATAGCGCTAGTAAACTATGCCTGCGGAATGCTGCCTTACACCCCAGTGCTGCCAACTCCGCCTTCACCACCATCACTCTTTGATAACCATGAAACCCAATGCCCTCAGCCCAGCATCCGCCCCCGCCCCCACCCCCGCCCTCGCCCCCACCCCCGCCCTCGCCCCCACCACAGGCACCGGCACAGACACGAGCAGAGTCACCAGCTCAAGCTCCCGATGTCACCAATGGAGGAGAATTGTTGCAAGTGGGTGCAGCAGTTGGATAGTGTATGCGTATGCGGGCTGCTATCCCGCTTGCCCGCCTTCCTCGAGAGGCCTCAGCATAACTTTTCTATTACTGTTGGCGGTTCTTGTGATGCTACATACTCATGTGGAGGAGGGATCAAA

Coding sequence (CDS)

ATGGCGAGGTCCGGGATCCTCGTACTGGTATTAGCGCTGTTCTTGGTGCTGGTTACCAACACAGAGAGTCAATGGCAGCCGCCAGAGGTGCCAGAGGTTGCTCCCCCTCCGCTCTGCGCCTCCCAGATAGCGCTAGTAAACTATGCCTGCGGAATGCTGCCTTACACCCCAGTGCTGCCAACTCCGCCTTCACCACCATCACTCTTTGATAACCATGAAACCCAATGCCCTCAGCCCAGCATCCGCCCCCGCCCCCACCCCCGCCCTCGCCCCCACCCCCGCCCTCGCCCCCACCACAGGCACCGGCACAGACACGAGCAGAGTCACCAGCTCAAGCTCCCGATGTCACCAATGGAGGAGAATTGTTGCAAGTGGGTGCAGCAGTTGGATAGTGTATGCGTATGCGGGCTGCTATCCCGCTTGCCCGCCTTCCTCGAGAGGCCTCAGCATAACTTTTCTATTACTGTTGGCGGTTCTTGTGATGCTACATACTCATGTGGAGGAGGGATCAAA

Protein sequence

MARSGILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLPTPPSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEENCCKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGGGIK
Homology
BLAST of MC09g0696 vs. NCBI nr
Match: XP_038899044.1 (uncharacterized protein LOC120086453 [Benincasa hispida])

HSP 1 Score: 215 bits (548), Expect = 1.15e-68
Identity = 112/171 (65.50%), Postives = 125/171 (73.10%), Query Frame = 0

Query: 1   MARSGILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLP 60
           M R  IL+  + LFL L+T+TE QWQPP+   VAP PLCASQIAL NYAC MLPY+ VLP
Sbjct: 1   MGRFKILIFSVTLFLALITDTEGQWQPPD---VAPRPLCASQIALANYACAMLPYSTVLP 60

Query: 61  TPPSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEE 120
            PP   SL DNHE+Q   P+ R R              H H+H H   HQ K+P+SPMEE
Sbjct: 61  PPPPSLSLSDNHESQG-SPNQRHR--------------HTHQHGHHHGHQQKIPLSPMEE 120

Query: 121 NCCKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGGGIK 171
           NCCKWVQQLDS C+CGLLSRLPAFLERPQHNFS+TVGGSCDATYSCGGGIK
Sbjct: 121 NCCKWVQQLDSECICGLLSRLPAFLERPQHNFSVTVGGSCDATYSCGGGIK 153

BLAST of MC09g0696 vs. NCBI nr
Match: XP_023548911.1 (uncharacterized protein LOC111807420 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176 bits (446), Expect = 3.28e-53
Identity = 95/169 (56.21%), Postives = 113/169 (66.86%), Query Frame = 0

Query: 1   MARSGILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLP 60
           M R  IL+  + LFL L+ +TE+QWQPP+   VAP P C SQIAL N AC MLPY+ +  
Sbjct: 1   MGRFKILLFAVTLFLALIPDTEAQWQPPK---VAPRPPCPSQIALANLACAMLPYSTMAL 60

Query: 61  TPPSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEE 120
           +PPS  SL DNHE+  P P+                  HRH H H   HQ ++ +SPMEE
Sbjct: 61  SPPSV-SLSDNHESHGPPPN------------------HRHLHTHWHGHQHQISLSPMEE 120

Query: 121 NCCKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGGG 169
           NCCKWVQQLDS C+C LLSRLPAFLERPQH  S +VGGSC+AT+SCGGG
Sbjct: 121 NCCKWVQQLDSQCLCRLLSRLPAFLERPQHKISFSVGGSCNATFSCGGG 147

BLAST of MC09g0696 vs. NCBI nr
Match: XP_031744964.1 (uncharacterized protein LOC116405195 [Cucumis sativus] >KGN44871.1 hypothetical protein Csa_016586 [Cucumis sativus])

HSP 1 Score: 175 bits (444), Expect = 6.40e-53
Identity = 92/168 (54.76%), Postives = 110/168 (65.48%), Query Frame = 0

Query: 1   MARSGILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLP 60
           M R  IL+L + LFL L+T++ESQWQPP    VAP PLCASQI L NYAC  LPY  + P
Sbjct: 1   MGRFKILILSVVLFLTLITDSESQWQPPG---VAPRPLCASQITLANYACATLPYAKLPP 60

Query: 61  TPPSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEE 120
            PP   SL +N ++Q                     P H H H H   H  K+P++P+EE
Sbjct: 61  PPPPSSSLSNNQDSQG-------------------SPVHGHHHGHHHRHHHKIPLTPIEE 120

Query: 121 NCCKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGG 168
           NCCKWVQQ+DS CVC LLSRLPAFL+RP HNFS+T+GGSC+ATY CGG
Sbjct: 121 NCCKWVQQVDSECVCELLSRLPAFLKRPIHNFSVTIGGSCNATYWCGG 146

BLAST of MC09g0696 vs. NCBI nr
Match: KAA0057606.1 (hypothetical protein E6C27_scaffold497G00830 [Cucumis melo var. makuwa] >TYK20987.1 hypothetical protein E5676_scaffold328G00120 [Cucumis melo var. makuwa])

HSP 1 Score: 161 bits (407), Expect = 1.77e-46
Identity = 84/147 (57.14%), Postives = 96/147 (65.31%), Query Frame = 0

Query: 24  QWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLPTPPSPPSLFDNHETQCPQPSIRP 83
           QWQPP    VAP PLCASQIAL NYAC  LPY+ +LP PP   SL  NH++Q        
Sbjct: 84  QWQPPA---VAPRPLCASQIALANYACATLPYSKLLPPPPPSSSLSSNHDSQG------- 143

Query: 84  RPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEENCCKWVQQLDSVCVCGLLSRLPA 143
                        P H H H H   H  K+P+SP+EENCCKWVQQ+DS CVC LLSRLPA
Sbjct: 144 ------------SPVHTHHHGHHHRHHHKIPLSPIEENCCKWVQQVDSECVCELLSRLPA 203

Query: 144 FLERPQHNFSITVGGS--CDATYSCGG 168
           FL+RP HNFS+ +GGS  C+ATYSCGG
Sbjct: 204 FLKRPIHNFSVIIGGSLSCNATYSCGG 208

BLAST of MC09g0696 vs. NCBI nr
Match: KAG7014427.1 (hypothetical protein SDJN02_24604, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 148 bits (373), Expect = 7.23e-42
Identity = 85/159 (53.46%), Postives = 103/159 (64.78%), Query Frame = 0

Query: 1   MARSGILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLP 60
           M R  IL+  + LFL L+ +TE+QWQPP+   VAP P C SQIAL N AC MLPY+ +  
Sbjct: 1   MERFKILIFAVTLFLALIPDTEAQWQPPK---VAPRPPCPSQIALANLACAMLPYSTMAL 60

Query: 61  TPPSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEE 120
           +PPS  SL DNHE+                 H  P P+H+H   H   HQ ++ +SPMEE
Sbjct: 61  SPPSL-SLSDNHES-----------------HGLP-PNHKHLRTHWHGHQHQISLSPMEE 120

Query: 121 NCCKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGS 159
           NCCKWVQQLDS C+C LLSRLPAFLERPQH  S +VGG+
Sbjct: 121 NCCKWVQQLDSQCLCRLLSRLPAFLERPQHKISFSVGGN 137

BLAST of MC09g0696 vs. ExPASy TrEMBL
Match: A0A0A0KAN7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392440 PE=4 SV=1)

HSP 1 Score: 175 bits (444), Expect = 3.10e-53
Identity = 92/168 (54.76%), Postives = 110/168 (65.48%), Query Frame = 0

Query: 1   MARSGILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLP 60
           M R  IL+L + LFL L+T++ESQWQPP    VAP PLCASQI L NYAC  LPY  + P
Sbjct: 1   MGRFKILILSVVLFLTLITDSESQWQPPG---VAPRPLCASQITLANYACATLPYAKLPP 60

Query: 61  TPPSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEE 120
            PP   SL +N ++Q                     P H H H H   H  K+P++P+EE
Sbjct: 61  PPPPSSSLSNNQDSQG-------------------SPVHGHHHGHHHRHHHKIPLTPIEE 120

Query: 121 NCCKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGG 168
           NCCKWVQQ+DS CVC LLSRLPAFL+RP HNFS+T+GGSC+ATY CGG
Sbjct: 121 NCCKWVQQVDSECVCELLSRLPAFLKRPIHNFSVTIGGSCNATYWCGG 146

BLAST of MC09g0696 vs. ExPASy TrEMBL
Match: A0A5A7UTY5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00120 PE=4 SV=1)

HSP 1 Score: 161 bits (407), Expect = 8.56e-47
Identity = 84/147 (57.14%), Postives = 96/147 (65.31%), Query Frame = 0

Query: 24  QWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLPTPPSPPSLFDNHETQCPQPSIRP 83
           QWQPP    VAP PLCASQIAL NYAC  LPY+ +LP PP   SL  NH++Q        
Sbjct: 84  QWQPPA---VAPRPLCASQIALANYACATLPYSKLLPPPPPSSSLSSNHDSQG------- 143

Query: 84  RPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEENCCKWVQQLDSVCVCGLLSRLPA 143
                        P H H H H   H  K+P+SP+EENCCKWVQQ+DS CVC LLSRLPA
Sbjct: 144 ------------SPVHTHHHGHHHRHHHKIPLSPIEENCCKWVQQVDSECVCELLSRLPA 203

Query: 144 FLERPQHNFSITVGGS--CDATYSCGG 168
           FL+RP HNFS+ +GGS  C+ATYSCGG
Sbjct: 204 FLKRPIHNFSVIIGGSLSCNATYSCGG 208

BLAST of MC09g0696 vs. ExPASy TrEMBL
Match: A0A6P4AZX7 (uncharacterized protein DDB_G0272724 OS=Ziziphus jujuba OX=326968 GN=LOC107426015 PE=3 SV=1)

HSP 1 Score: 125 bits (315), Expect = 9.59e-34
Identity = 71/160 (44.38%), Postives = 88/160 (55.00%), Query Frame = 0

Query: 15  LVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTPVLPTPPSPPSLFD---- 74
           L+LV   ESQ  PP    VAP PLCASQ AL +YAC ++P+TP+ P PPSPPS  D    
Sbjct: 7   LLLVPKLESQVLPP----VAPRPLCASQFALASYACSLIPFTPLPPEPPSPPSPPDFLGH 66

Query: 75  --NHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEENCCKWVQQ 134
              H                          HRH H H   H+ +   SP EE+CC+W++Q
Sbjct: 67  SHGHGHG-----------------------HRHGHGHRHGHRHRHRESPAEESCCRWLKQ 126

Query: 135 LDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGG 168
           LDS CVC +L  LP FL RP H +S+ VG +CD T+SC G
Sbjct: 127 LDSQCVCDVLVHLPNFLSRPAHEYSVIVGDACDVTFSCEG 139

BLAST of MC09g0696 vs. ExPASy TrEMBL
Match: A0A0B2QE80 (Uncharacterized protein OS=Glycine soja OX=3848 GN=D0Y65_007235 PE=4 SV=1)

HSP 1 Score: 125 bits (313), Expect = 1.92e-33
Identity = 73/167 (43.71%), Postives = 94/167 (56.29%), Query Frame = 0

Query: 5   GILVLVLALFLVLVTNTESQWQPPEVPEVAPPPLCASQIALVNYACGMLPYTP-VLPTPP 64
            ++ L L LFL +V   ESQ +P  +P+  P PLCASQ ALVNYAC  LP++P V P  P
Sbjct: 5   NLIALALTLFLAIVPKMESQIKP--IPKT-PRPLCASQFALVNYACSRLPFSPGVPPDSP 64

Query: 65  SPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEENCC 124
           SP    +NH  +                       HRH HRH   H+ +   +P E+NCC
Sbjct: 65  SPDEENNNHHNRS----------------------HRHGHRH--GHRHRHHQTPDEDNCC 124

Query: 125 KWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGGGI 170
           +W +++D  CVC LL RLP FL RP H +++ VG SCD TYSCG  I
Sbjct: 125 RWAKEVDHQCVCELLLRLPPFLIRPSHQYTLKVGASCDITYSCGAPI 144

BLAST of MC09g0696 vs. ExPASy TrEMBL
Match: A0A4D6MCY0 (Uncharacterized protein OS=Vigna unguiculata OX=3917 GN=DEO72_LG6g2365 PE=4 SV=1)

HSP 1 Score: 125 bits (313), Expect = 2.23e-33
Identity = 70/168 (41.67%), Postives = 87/168 (51.79%), Query Frame = 0

Query: 7   LVLVLALFLVLVTNTESQWQPPEVPEV-APPPLCASQIALVNYACGMLPY---TPVLPTP 66
           L+ V  L L L   TESQ +PP +P   AP PLC SQ ALVNYAC  LP+    P   +P
Sbjct: 6   LITVALLLLALAPKTESQLKPPLIPVAKAPRPLCGSQFALVNYACSRLPFRHGAPPADSP 65

Query: 67  PSPPSLFDNHETQCPQPSIRPRPHPRPRPHPRPRPHHRHRHRHEQSHQLKLPMSPMEENC 126
           P P    + H                          H HRH H   H  +   +P EENC
Sbjct: 66  PPPEGDGERHHN------------------------HNHRHNHRHGHSHRHHQTPEEENC 125

Query: 127 CKWVQQLDSVCVCGLLSRLPAFLERPQHNFSITVGGSCDATYSCGGGI 170
           C+W +++D+ CVC LL RLP FL RP H +++ VG +CD TYSCG  I
Sbjct: 126 CRWAKEVDNQCVCELLLRLPPFLVRPVHQYTLNVGAACDITYSCGSPI 149

BLAST of MC09g0696 vs. TAIR 10
Match: AT3G63095.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.2e-13
Identity = 72/250 (28.80%), Postives = 94/250 (37.60%), Query Frame = 0

Query: 7   LVLVLALFLVLVTNTESQWQPPEVPEVAPP-PLCASQIALVNYACGMLPYTPVLP----T 66
           L + + L L L + T  Q     V ++ PP PLCASQ AL NYAC  LP   V P    T
Sbjct: 4   LFIAVTLALALSSFTRGQ----RVIQIPPPRPLCASQYALANYACSRLPMHTVPPPAPIT 63

Query: 67  PP-----SPPSLFDNH---------------------------------------ETQCP 126
           PP      PP   D+H                                       +    
Sbjct: 64  PPPAPIAPPPPEHDHHDHDHDHDHDHDHDDDHDDDDDHDHDDDHDHDHDDDHDHDDDDRD 123

Query: 127 QPSIRPRPHPRPRPHPRP---------------------------------------RPH 169
           +   R R   R R H R                                        R H
Sbjct: 124 RDRDRDRDRDRDRDHDRAHDDHDHNHDHDNDHNDNNHDHNHDADHHNNDDNSHDQHRRRH 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_038899044.11.15e-6865.50uncharacterized protein LOC120086453 [Benincasa hispida][more]
XP_023548911.13.28e-5356.21uncharacterized protein LOC111807420 [Cucurbita pepo subsp. pepo][more]
XP_031744964.16.40e-5354.76uncharacterized protein LOC116405195 [Cucumis sativus] >KGN44871.1 hypothetical ... [more]
KAA0057606.11.77e-4657.14hypothetical protein E6C27_scaffold497G00830 [Cucumis melo var. makuwa] >TYK2098... [more]
KAG7014427.17.23e-4253.46hypothetical protein SDJN02_24604, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A0A0KAN73.10e-5354.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392440 PE=4 SV=1[more]
A0A5A7UTY58.56e-4757.14Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6P4AZX79.59e-3444.38uncharacterized protein DDB_G0272724 OS=Ziziphus jujuba OX=326968 GN=LOC10742601... [more]
A0A0B2QE801.92e-3343.71Uncharacterized protein OS=Glycine soja OX=3848 GN=D0Y65_007235 PE=4 SV=1[more]
A0A4D6MCY02.23e-3341.67Uncharacterized protein OS=Vigna unguiculata OX=3917 GN=DEO72_LG6g2365 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G63095.15.2e-1328.80Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..111
NoneNo IPR availablePANTHERPTHR34377TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEINcoord: 9..170
NoneNo IPR availablePANTHERPTHR34377:SF6SUBFAMILY NOT NAMEDcoord: 9..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0696.1MC09g0696.1mRNA