MC09g0654 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0654
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionE3 ubiquitin-protein ligase MIEL1
LocationMC09: 5940433 .. 5944007 (+)
RNA-Seq ExpressionMC09g0654
SyntenyMC09g0654
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTATACAATTTAACGTAAAACAACTTAGAATTTTTTGGTCCTGTGATAATTCTTCTCAAATCGAGGAGCACGAACTGGGCGGTCTCCAATGGAGAAAGAGGACTCCTCTGTGCCTCAATCTGCCAGGAGAGAAGATTTCGGAAAATTTCAGCACGGGTATGAAATTTCTTTCCTCCATTGTTGTCTCAACTCGAGGTTTCCTTGGTTTTTTTGACCGAGTTATTGTTCTTGCTTTCATGACCAGTAAGTTGTTTTTGATTTCTATTAGATGCGAGCATTACAGGAGACGATGCAGAATCCGAGCCCCATGCTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGGTATTTCTCTCTTCTCTGTGTCCTTGCGTTTCGTGCGTGTGTTTGATATAGAGAGATGCATTAGAAAGGATTGCGTTTATGTTTGTTAAAGTGGAGCTTTGAGTAATTCTGTAAAGAAGTTCTCTGAATCTTTCTCTAATTATCCCTGATTTTAGAGCTCGCTGAGCAATCCGAAGGACCGTCATGAACTTGTTCGGCAGGACGTTAGACAAGTATGTTTCTTATAATCCTTTTAAGTACACTGTTTTATTGATTTTTTTGAGTAATCGTATCTGATGGGATTTGTTTGGTTGATCAATTTTGCTCTGGAATTGATGTTTTAGGTGGTTTGCTTGATTTGCAATACAGAGCAAGAGGTTTGGGGGCTTTTTTTCTGGCTAATTTTGTTCCTTGCGTTGATTTATTCCTTTTTTTTCCATTCCTTTTCTTTTTCTGCTAACCGTTTCAATTTTGTACATTTTAGGTTGCCAAGGTTTGTCGAAAGTGTAATGTTAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTTTGATGATGATGTAAGAGAAGTTTCCATCAACCTGTCTTTTTTTCCCCCTTCTCTTTCTTGAACAACTTTAAAAGTTTGAAATGTGGAATGGAATGATTTTTTTATCCTATATACTGATAGTTTCTGTTATGAATGCAGACCAAGAAGAAGCAGTTTCATTGTGATGCATGTGGTATCTGTAGGTTAGTGTGCTGTTTGCGTATTCTTATGTAATTCAGCTGTTTCTGTTATGTTTCTCAACACAAGTTTTTTTTGTTATACGATTTTTAGAGTTGGTGGCCGAGAGAATTTCTTTCACTGTGAAAAATGTGGTATGTTATTCAACTATCTTGATGTTTTGTTATAAACTAGTATTATGTTATTGTTTGCAGTATGTAGCAGAAGAGTGTTGTGTGTTTGTCTGTAAAGTCCTCATTCGTATGTAATAAACCACGTTCGCACATTCACATAAGATTGATATGCATTGGTAAAAGGTGAATAATTGAATTGAATGGTTTATTTAGAAGAATGAAAGTAGTATTGTGCTCATACTGCATGCAGTTTCCATCAGTTTTTTGCTTGTGATGTTTTCAGTGTTAATGCAACTAATTCGTTCCTTCTTGTGATGTTTTCAGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACTTGTGTGTTGAGAATTCAATGAAAAGTTTTTGCCCTATCTGTTATGAGGTTTGTGATTTCAGAATATATGTTTTCTGTGGGAGTACAATCTAAACTGTTCTCTTCTAACAATTTTGTTGATGCCTGCAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCGCTGCGGTCACACAATACATTTGGATTGTTTCAGGGAGATGGAATCGCAGAATCAGTAATCCAAACTCTTTATTCAAATCCAGTTTCTGAATCTACTTTCAAAATATTTCCAAAAACTTGGAAAATACAAAACACAACTCAAATTTTCTATTATATCCATTGCCTTCTATTCTCAAACCAAAGAGGCTCTAGTTTCTTATTTTGAATATTACAGATTTCCACTTCTTCTCCAATTCTAATGTATTAACATTTGTCTCTCAGATACCGTTGTCCTATCTGCTCGAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGGTGTGCAGTTTCTCTTTTGTTTCACATTTCTATAGGTTTATTTGTAAATTCTTAGTTGTGCAAGGCAATTAGATAACTTTCATTTTAATCCTTAGAGAGGTTTCTGCAGTTAGCGAACATGGTGGAACAGGTGGCTTCACATAAGTTTTAGCAAGTTTAGAATCTATATTTTATCATTATATATTGTACCCACTTTTCGACCTCCTGACTGATCCTTTCATTTCTTTCTTTTTCATGATTTAGATGTATGTTTATACGTTACCCAAGTCTCTGTCTATAGCATTGGGTTGATTCTTAGAAAGTTGGTGGTTGCAGACTTATTATCTTATTAGAAATTCCATGAAGTGAGAGAAAAAAAGTTAAGATAGGAGTGTAGATTCAAGGCTTTAGTTATATATTCCAGTTGGGTTGAGAGGGATAGAGAGACAGTAACATACAATAAAGATGAAAGAGGAAAATAGCAGGCCAGAGCTAAATACATTCTGTTTCGGGCGAATATGTTTTTGACTTGCTCTGTCTACGAACTTAAATTATGTTCTTCTTTGAACAATGGTTTCTTCGAATTATTTTCCTCATATCTTATTTACCCATTACCTGTTAGCTAGTTGATATATTATGGTATTCTATTTCTTCTTCTGTCAAGTTCTGGTAGTGAAATTCCAAAGTTTCTTTGGATTCTGAATTATCTTTACTGTACCTTACATCTTTTTTTGCTACATATGGTCTTAAATTTTAAAATCTTTCCATCTTATCTATAGATTGACTGCACACCAATGCCTGAAGAATACAGCCATGAGGTAGTTTCTTGTTTACACGTTTAGATTACCACAAGCTATGTGTTCTTCTTTATCACTCTGCATGTTACAAGTCTTCTAACTTAAACCAACTACCGGGTTAAACAGGTATCGATTCTCTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGATCATTAATGAAAACTGAAGGTAAACAACAAATATGGGGCTCTCTTAATCTTAACAATTAGGTTTTTTGTTGAAAGTTTGATTATTGGAGTCATTTGATCCATTTAGGAAGTCAACCCGTTTTGTTCAGAGGCTGACTAAGATTGGCTAAACCCATGAAGGGAAGTTTATAGTGCAACATGAAGTGAAATGGTGTTATCTTCATTAGGAAGAATCCCAGATTTTTTTTTTCTTTCATTGTATCATTTAATATTCATATTGAAAAAATATTATATCATATTAGATACCTTATGCCGATGTGAGTATATCATAAATCTTAATGAATCCTGGTCTAAAAACTTGAACCTCAAATATAGCAAGAAATAGATAGTTGAATGATTATTTATTTCTCTTAAAAAGGATATAGTTCGGGGGATGGATGTTAAGGGCCGTAATGTTCTCGTTTCTTACTTAGGACATGAGAGTGTACAGATATATGGTAGTGCCACCGGTTCATAGTTTTATCACATACAAGTTAGCATGCGAT

mRNA sequence

ATTTTATACAATTTAACGTAAAACAACTTAGAATTTTTTGGTCCTGTGATAATTCTTCTCAAATCGAGGAGCACGAACTGGGCGGTCTCCAATGGAGAAAGAGGACTCCTCTGTGCCTCAATCTGCCAGGAGAGAAGATTTCGGAAAATTTCAGCACGGATGCGAGCATTACAGGAGACGATGCAGAATCCGAGCCCCATGCTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGAGCTCGCTGAGCAATCCGAAGGACCGTCATGAACTTGTTCGGCAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAATACAGAGCAAGAGGTTGCCAAGGTTTGTCGAAAGTGTAATGTTAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTTTGATGATGATACCAAGAAGAAGCAGTTTCATTGTGATGCATGTGGTATCTGTAGAGTTGGTGGCCGAGAGAATTTCTTTCACTGTGAAAAATGTGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACTTGTGTGTTGAGAATTCAATGAAAAGTTTTTGCCCTATCTGTTATGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCGCTGCGGTCACACAATACATTTGGATTGTTTCAGGGAGATGGAATCGCAGAATCAATACCGTTGTCCTATCTGCTCGAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCAATGCCTGAAGAATACAGCCATGAGGTATCGATTCTCTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGATCATTAATGAAAACTGAAGGAAGTCAACCCGTTTTGTTCAGAGGCTGACTAAGATTGGCTAAACCCATGAAGGGAAGTTTATAGTGCAACATGAAGTGAAATGGTGTTATCTTCATTAGGAAGAATCCCAGATTTTTTTTTTCTTTCATTGTATCATTTAATATTCATATTGAAAAAATATTATATCATATTAGATACCTTATGCCGATGTGAGTATATCATAAATCTTAATGAATCCTGGTCTAAAAACTTGAACCTCAAATATAGCAAGAAATAGATAGTTGAATGATTATTTATTTCTCTTAAAAAGGATATAGTTCGGGGGATGGATGTTAAGGGCCGTAATGTTCTCGTTTCTTACTTAGGACATGAGAGTGTACAGATATATGGTAGTGCCACCGGTTCATAGTTTTATCACATACAAGTTAGCATGCGAT

Coding sequence (CDS)

ATGGAGAAAGAGGACTCCTCTGTGCCTCAATCTGCCAGGAGAGAAGATTTCGGAAAATTTCAGCACGGATGCGAGCATTACAGGAGACGATGCAGAATCCGAGCCCCATGCTGCGACCGAATCTTTACCTGTCGCCATTGCCACAACGAGGCCATGAGCTCGCTGAGCAATCCGAAGGACCGTCATGAACTTGTTCGGCAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAATACAGAGCAAGAGGTTGCCAAGGTTTGTCGAAAGTGTAATGTTAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTTTGATGATGATACCAAGAAGAAGCAGTTTCATTGTGATGCATGTGGTATCTGTAGAGTTGGTGGCCGAGAGAATTTCTTTCACTGTGAAAAATGTGGATCTTGCTATTCCACTCCTCTGCGTGATAACCACTTGTGTGTTGAGAATTCAATGAAAAGTTTTTGCCCTATCTGTTATGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCGCTGCGGTCACACAATACATTTGGATTGTTTCAGGGAGATGGAATCGCAGAATCAATACCGTTGTCCTATCTGCTCGAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCAATGCCTGAAGAATACAGCCATGAGGTATCGATTCTCTGCAACGACTGCAGCCATACGAGCAAAGTTCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGGAGATCATTAA

Protein sequence

MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSKVRFHILGHKCSQCNSFNTRRISTGDH
Homology
[Close]
BLAST of MC09g0654 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-104
Identity = 161/254 (63.39%), Postives = 205/254 (80.71%), Query Frame = 0 

Query: 14  REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVV 73
           R  FGK   GC+HY+RRC+IRAPCC+ +F CRHCHNE+ S+L N  DRH+LVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ  A+VC  C VNMGEYFC IC F+DDDT+K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHLDCFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM+CGHT+H++C+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSKVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T++V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]
BLAST of MC09g0654 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 5.6e-91
Identity = 145/267 (54.31%), Postives = 184/267 (68.91%), Query Frame = 0 

Query: 4   EDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLS-NPKDRH 63
           E+    + A   + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RH
Sbjct: 7   ENEQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRH 66

Query: 64  ELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGIC 123
           EL R +V +V+C +C TEQ+V + C  C V MG+YFC  CKFFDDD  KKQ+HCD CGIC
Sbjct: 67  ELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGIC 126

Query: 124 RVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTI 183
           R GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+RCGHT+
Sbjct: 127 RTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTM 186

Query: 184 HLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSH 243
           HL+C ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC  
Sbjct: 187 HLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGS 246

Query: 244 TSKVRFHILGHKCSQCNSFNTRRISTG 269
            + VRFH++ HKCS C S+NTR+   G
Sbjct: 247 NTNVRFHLIAHKCSSCGSYNTRQTQRG 273

[Close]
BLAST of MC09g0654 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.2e-90
Identity = 145/265 (54.72%), Postives = 189/265 (71.32%), Query Frame = 0 

Query: 2   EKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLS-NPKD 61
           EK +   P    + + G  Q+GC HYRRRCRIRAPCC+ IF CRHCHNE  +S+  +   
Sbjct: 39  EKTEDVDPDDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVK 98

Query: 62  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 121
           RHEL R +V+QV+C +C TEQEV +VC  C V MG+YFC++CK FDDD  K+Q+HC+ CG
Sbjct: 99  RHELPRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCG 158

Query: 122 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 181
           ICR+GG+ENFFHC KCG CYS  L+++H CVE +M   CPIC+E+LF+S  D +V+ CGH
Sbjct: 159 ICRIGGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGH 218

Query: 182 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 241
           TIH+ C REME   Q+ CP+CSK+V DMS +W  L  E+  T      +  V ILCNDC 
Sbjct: 219 TIHVKCLREMEEHCQFACPLCSKSVCDMSKAWERLDEEL-ATISDTCDNKMVRILCNDCG 278

Query: 242 HTSKVRFHILGHKCSQCNSFNTRRI 266
            TS+V+FH++ HKC +C S+NTR+I
Sbjct: 279 ATSEVQFHLIAHKCQKCKSYNTRQI 302

[Close]
BLAST of MC09g0654 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 1.8e-89
Identity = 147/266 (55.26%), Postives = 185/266 (69.55%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME +D+S      R  FG+   GC+HYRRRCRIRAPCC+ +F CRHCHNE      + KD
Sbjct: 1   MELDDAS------RHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNE------STKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
            HEL R  V  V+CL+C+TEQ VA+VC  C V MGEYFC  CKFFDDD  ++ FHC  CG
Sbjct: 61  GHELDRHAVESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGG++NFFHCEKCGSCYS  LRD H C+ENSMK+ CPICYE+LFDS+++T+V+RCGH
Sbjct: 121 ICRVGGKDNFFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           T+HL CF EM   +++ CPICS  + DM      L  EI+   +  +Y  +  I+CNDC 
Sbjct: 181 TMHLQCFHEMLKHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMGKGWIVCNDCR 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIS 267
            T++V   + GHKC  C S NT R++
Sbjct: 241 DTTQVYARVAGHKCCHCQSHNTCRVA 254

[Close]
BLAST of MC09g0654 vs. ExPASy Swiss-Prot
Match: Q96PM5 (RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=RCHY1 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.5e-64
Identity = 116/252 (46.03%), Postives = 154/252 (61.11%), Query Frame = 0 

Query: 21  QHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVVCLICNTE 80
           Q GCEHY R C ++APCCD+++TCR CH+          + H+L R  V++V C+ C   
Sbjct: 17  QRGCEHYDRGCLLKAPCCDKLYTCRLCHDN--------NEDHQLDRFKVKEVQCINCEKI 76

Query: 81  QEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCY 140
           Q   + C +C+   GEY+CDIC  FD D  KKQ+HC+ CGICR+G +E+FFHC KC  C 
Sbjct: 77  QHAQQTCEECSTLFGEYYCDICHLFDKD--KKQYHCENCGICRIGPKEDFFHCLKCNLCL 136

Query: 141 STPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHLDCFREMESQNQYRCPI 200
           +  L+  H C+EN  +  CPIC E +  S     V+ CGH +H  C+ EM  +  YRCP+
Sbjct: 137 AMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEG-YRCPL 196

Query: 201 CSKTVMDMSASWALLKMEIDCTPMPEEYSH-EVSILCNDCSHTSKVRFHILGHKCSQCNS 260
           C  + +DM+  W  L  E+  TPMP EY +  V ILCNDC+  S V+FHILG KC  C S
Sbjct: 197 CMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICES 256

Query: 261 FNT-----RRIS 267
           +NT     RRIS
Sbjct: 257 YNTAQAGGRRIS 257

[Close]
BLAST of MC09g0654 vs. NCBI nr
Match: XP_022150148.1 (E3 ubiquitin-protein ligase MIEL1 [Momordica charantia] >XP_022150149.1 E3 ubiquitin-protein ligase MIEL1 [Momordica charantia])

HSP 1 Score: 588 bits (1515), Expect = 1.58e-212
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDH 270
           HTSKVRFHILGHKCSQCNSFNTRRISTGDH
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDH 270

[Close]
BLAST of MC09g0654 vs. NCBI nr
Match: XP_022953225.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562 bits (1448), Expect = 2.91e-202
Identity = 257/269 (95.54%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAK C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of MC09g0654 vs. NCBI nr
Match: XP_023549279.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560 bits (1444), Expect = 1.19e-201
Identity = 256/269 (95.17%), Postives = 262/269 (97.40%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAK C KCNVNMGEYFC ICKF+DDDT+K QFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCSKCNVNMGEYFCGICKFYDDDTRKMQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of MC09g0654 vs. NCBI nr
Match: XP_022992470.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima])

HSP 1 Score: 560 bits (1444), Expect = 1.19e-201
Identity = 255/269 (94.80%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+K C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of MC09g0654 vs. NCBI nr
Match: XP_023004622.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquitin-protein ligase MIEL1 [Cucurbita pepo subsp. pepo] >KAG6593162.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551 bits (1421), Expect = 3.54e-198
Identity = 251/267 (94.01%), Postives = 257/267 (96.25%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME++D  VPQSA REDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  +VCR CNVNMGEYFCDICKF+DDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267

[Close]
BLAST of MC09g0654 vs. ExPASy TrEMBL
Match: A0A6J1D947 (E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400 PE=4 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 7.65e-213
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDH 270
           HTSKVRFHILGHKCSQCNSFNTRRISTGDH
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGDH 270

[Close]
BLAST of MC09g0654 vs. ExPASy TrEMBL
Match: A0A6J1GP21 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC111455836 PE=4 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 1.41e-202
Identity = 257/269 (95.54%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEVAK C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of MC09g0654 vs. ExPASy TrEMBL
Match: A0A6J1JVS4 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC111488790 PE=4 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 5.74e-202
Identity = 255/269 (94.80%), Postives = 263/269 (97.77%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME+EDS VPQSA REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQEV+K C KCNVNMGEYFC ICKF+DDDT+KKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269
           HTSKVRFHILGHKCSQCNSFNTRRISTGD
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRISTGD 269

[Close]
BLAST of MC09g0654 vs. ExPASy TrEMBL
Match: A0A6J1KQY2 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE=4 SV=1)

HSP 1 Score: 551 bits (1421), Expect = 1.71e-198
Identity = 251/267 (94.01%), Postives = 257/267 (96.25%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME++D  VPQSA REDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  +VCR CNVNMGEYFCDICKF+DDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267

[Close]
BLAST of MC09g0654 vs. ExPASy TrEMBL
Match: A0A6J1H4Y6 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 4.04e-197
Identity = 249/267 (93.26%), Postives = 256/267 (95.88%), Query Frame = 0 

Query: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60
           ME++D  VPQSA REDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAMSSLSNP D
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPND 60

Query: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  +VCR CNVNMGEYFCDICKF+DDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCS+CNSFNTRRIST
Sbjct: 241 HTSNVRFHILGHKCSRCNSFNTRRIST 267

[Close]
BLAST of MC09g0654 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 421.4 bits (1082), Expect = 5.5e-118
Identity = 182/263 (69.20%), Postives = 217/263 (82.51%), Query Frame = 0 

Query: 5   DSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKDRHEL 64
           DSS+P   R +DFGKFQ GCEHY+RRC+IRAPCC+ IF+CRHCHN++ +SL +PK+RH+L
Sbjct: 19  DSSIP---RDKDFGKFQFGCEHYKRRCKIRAPCCNLIFSCRHCHNDSANSLPDPKERHDL 78

Query: 65  VRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRV 124
           VRQ+V+QVVC IC TEQEVAKVC  C VNMGEYFCDICKFFDDD  K+QFHCD CGICRV
Sbjct: 79  VRQNVKQVVCSICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQFHCDDCGICRV 138

Query: 125 GGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHL 184
           GGR+ FFHC+ CG+CY   LRD H C+ENS K+ CP+CYE+LFDS+K   VM+CGHT+H+
Sbjct: 139 GGRDKFFHCQNCGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAAHVMKCGHTMHM 198

Query: 185 DCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSK 244
           DCF +M ++NQYRCPIC+K+++DMS SW LL  EI  T MP EY  EVSILCNDC+  SK
Sbjct: 199 DCFEQMINENQYRCPICAKSMVDMSPSWHLLDFEISATEMPVEYKFEVSILCNDCNKGSK 258

Query: 245 VRFHILGHKCSQCNSFNTRRIST 268
             FHILGHKCS C S+NTRRIST
Sbjct: 259 AMFHILGHKCSDCGSYNTRRIST 278

[Close]
BLAST of MC09g0654 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.1e-105
Identity = 161/254 (63.39%), Postives = 205/254 (80.71%), Query Frame = 0 

Query: 14  REDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVV 73
           R  FGK   GC+HY+RRC+IRAPCC+ +F CRHCHNE+ S+L N  DRH+LVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ  A+VC  C VNMGEYFC IC F+DDDT+K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHLDCFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM+CGHT+H++C+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSKVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T++V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]
BLAST of MC09g0654 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 335.5 bits (859), Expect = 4.0e-92
Identity = 145/267 (54.31%), Postives = 184/267 (68.91%), Query Frame = 0 

Query: 4   EDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLS-NPKDRH 63
           E+    + A   + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RH
Sbjct: 7   ENEQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRH 66

Query: 64  ELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGIC 123
           EL R +V +V+C +C TEQ+V + C  C V MG+YFC  CKFFDDD  KKQ+HCD CGIC
Sbjct: 67  ELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGIC 126

Query: 124 RVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTI 183
           R GG ENFFHC++C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+RCGHT+
Sbjct: 127 RTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTM 186

Query: 184 HLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSH 243
           HL+C ++M   N+Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC  
Sbjct: 187 HLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGS 246

Query: 244 TSKVRFHILGHKCSQCNSFNTRRISTG 269
            + VRFH++ HKCS C S+NTR+   G
Sbjct: 247 NTNVRFHLIAHKCSSCGSYNTRQTQRG 273

[Close]
BLAST of MC09g0654 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.5e-91
Identity = 143/251 (56.97%), Postives = 179/251 (71.31%), Query Frame = 0 

Query: 16  DFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLS-NPKDRHELVRQDVRQVVC 75
           D G  ++GC HYRRRC IRAPCC+ IF C HCH EA ++++ + K RH++ R  V QV+C
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVIC 114

Query: 76  LICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRVGGRENFFHCE 135
           L+C TEQEV ++C  C V MG+YFC +CK +DDDT KKQ+HCD CGICR+GGRENFFHC 
Sbjct: 115 LLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHCY 174

Query: 136 KCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHLDCFREMESQN 195
           KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+ CGHTIH  C  EM    
Sbjct: 175 KCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDHY 234

Query: 196 QYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTSKVRFHILGHK 255
           QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   ++V++H++  K
Sbjct: 235 QYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVVAQK 294

Query: 256 CSQCNSFNTRR 265
           C  C S+NTR+
Sbjct: 295 CPNCKSYNTRQ 305

[Close]
BLAST of MC09g0654 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 325.5 bits (833), Expect = 4.1e-89
Identity = 143/261 (54.79%), Postives = 179/261 (68.58%), Query Frame = 0 

Query: 16  DFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLS-NPKDRHELVRQDVR---- 75
           D G  ++GC HYRRRC IRAPCC+ IF C HCH EA ++++ + K RH++ R  V     
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQLTR 114

Query: 76  ------QVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACGICRV 135
                 QV+CL+C TEQEV ++C  C V MG+YFC +CK +DDDT KKQ+HCD CGICR+
Sbjct: 115 PLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRI 174

Query: 136 GGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGHTIHL 195
           GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+ CGHTIH 
Sbjct: 175 GGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQ 234

Query: 196 DCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTS 255
            C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   +
Sbjct: 235 KCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKA 294

Query: 256 KVRFHILGHKCSQCNSFNTRR 265
           +V++H++  KC  C S+NTR+
Sbjct: 295 EVQYHVVAQKCPNCKSYNTRQ 315

The following BLAST results are available for this feature:
BLAST of MC09g0654 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8VZK01.5e-10463.39E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q9FFB65.6e-9154.31E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q5JL966.2e-9054.72Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q10LI11.8e-8955.26E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q96PM54.5e-6446.03RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9... [more]
BLAST of MC09g0654 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022150148.11.58e-212100.00E3 ubiquitin-protein ligase MIEL1 [Momordica charantia] >XP_022150149.1 E3 ubiqu... [more]
XP_022953225.12.91e-20295.54E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubi... [more]
XP_023549279.11.19e-20195.17E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo][more]
XP_022992470.11.19e-20194.80E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima][more]
XP_023004622.13.54e-19894.01E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquiti... [more]
BLAST of MC09g0654 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D9477.65e-213100.00E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400... [more]
A0A6J1GP211.41e-20295.54E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1JVS45.74e-20294.80E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC1114887... [more]
A0A6J1KQY21.71e-19894.01E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE... [more]
A0A6J1H4Y64.04e-19793.26E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 ... [more]
BLAST of MC09g0654 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G62970.15.5e-11869.20zinc finger (C3HC4-type RING finger) family protein [more]
AT5G18650.11.1e-10563.39CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G22920.14.0e-9254.31CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.11.5e-9156.97CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.34.1e-8954.79CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 159..201
e-value: 4.7E-7
score: 39.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 159..202
score: 11.913911
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 24..104
e-value: 2.7E-20
score: 72.6
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 17..92
score: 24.551851
NoneNo IPR availableGENE3D2.20.28.10coord: 229..269
e-value: 1.7E-17
score: 64.4
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 15..265
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 158..209
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 159..199
e-value: 2.2E-7
score: 30.8
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 206..263
e-value: 1.6E-23
score: 82.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 146..222
e-value: 2.9E-10
score: 41.2
IPR031109Ring finger and CHY zinc finger domain-containing protein, plantPANTHERPTHR21319:SF12ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEINcoord: 15..265
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 94..158
score: 33.23344
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 20..93
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 95..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0654.1MC09g0654.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding