MC09g0644 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0644
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionOTU domain-containing protein 6B
LocationMC09: 5895776 .. 5896960 (-)
RNA-Seq ExpressionMC09g0644
SyntenyMC09g0644
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATCTGCATGCAGGAAAGAGATATCACAGTTGCAGGACAAAGAAATTGAACTTAAAAAGACAGCAGCTAGAGGTAGCAAAGCTAAACAAGGAGTGAAGAAGAAACAGGTGGAGGAAGAGATATCTCAACTTTTTGCTAAGCTAAAAAAGAAACACACTGAAGAACTTGCGTCGTTAGGCTATAGTAACAAGAACAGCAACAGTAATGGAAATGAAAAAAATAACCTTGACAATCTAGTGAAGGCCATAGCTGGAGTGTCGATGACAGCTCAATCTGACCATTCAAAGCCCAGCAAAAGCACGAAGAGGAGGGAGAAGAGGGCTCAACAAGAAGCGGAAAGAGACAGAAGAATCGAAGAGGAGCAGAGCAACATTGTGAGTAATAGAATGGTTGAGAATGAGCAGTTGGAAAAGAAGCTTGGACCTCTTGGTTTGACTGTTCACGAAATCAAGCCAGATGGGCATTGCCTCTATCGAGCAGTCGAAAACCAGCTCACCTTCCTTTCCGGTGGTTCGCCTCCATACACATTCCAAGAACTTCGCAAGATGATGGCAGTTTATATGAGAGACTGTTAAGGATCCCACGTTGAAAAAAGAAAGGACCTTACGATGATTTATAATAAATATGGGCTACTCCTCTCATTCCCAATTGGTTTTGAGATGGAACCTCATGAATATTAATATGGTATCAGAATCCAAAACGCTTAAACGAGCAACCGATCCAAAAATGGGATTCGGCCAAGATCGATGAGCTAAAAGAGTCACGATCTTGAAGGGGCATGTAAAGGATCTCACATTGAAAAGAGAAGAGACCTTACAATGATTTATAATAAACATTGGCTACTCCTCTCATTGCCAATTGATTTTGAGATGGAACTCCATGAATATTAATAGAGACCATTCACCTAATTACATGCCATTTTTTCTCTCAGACAACACCATAGATGGCAACACCGATGATTTGAAAATTACTGCAAGGAAGTCGAGTCAACAGCAGCGTGGGGAGGGCAGCTGGAGCTCGGAGCTTTGACCCATTGCCTTAAAAAACATATCATGATATTTTCAGGATCCTTCCCAGAAGTGGAAATGGGGAAGGAATACAAATCAGAGGGCTTAGTGAAGAGGCTGTCGTACCATAAGCATGCCTTTGGACTTGGGGAGCACTACAATTCTGTGGTTCCTGTA

mRNA sequence

TTATCTGCATGCAGGAAAGAGATATCACAGTTGCAGGACAAAGAAATTGAACTTAAAAAGACAGCAGCTAGAGGTAGCAAAGCTAAACAAGGAGTGAAGAAGAAACAGGTGGAGGAAGAGATATCTCAACTTTTTGCTAAGCTAAAAAAGAAACACACTGAAGAACTTGCGTCGTTAGGCTATAGTAACAAGAACAGCAACAGTAATGGAAATGAAAAAAATAACCTTGACAATCTAGTGAAGGCCATAGCTGGAGTGTCGATGACAGCTCAATCTGACCATTCAAAGCCCAGCAAAAGCACGAAGAGGAGGGAGAAGAGGGCTCAACAAGAAGCGGAAAGAGACAGAAGAATCGAAGAGGAGCAGAGCAACATTGTGAGTAATAGAATGGTTGAGAATGAGCAGTTGGAAAAGAAGCTTGGACCTCTTGGTTTGACTGTTCACGAAATCAAGCCAGATGGGCATTGCCTCTATCGAGCAGTCGAAAACCAGCTCACCTTCCTTTCCGGTGGTTCGCCTCCATACACATTCCAAGAACTTCGCAAGATGATGGCAGTTTATATGAGAGACTACCATTCACCTAATTACATGCCATTTTTTCTCTCAGACAACACCATAGATGGCAACACCGATTTTGAAAATTACTGCAAGGAAGTCGAGTCAACAGCAGCGTGGGGAGGGCAGCTGGAGCTCGGAGCTTTGACCCATTGCCTTAAAAAACATATCATGATATTTTCAGGATCCTTCCCAGAAGTGGAAATGGGGAAGGAATACAAATCAGAGGGCTTAGTGAAGAGGCTGTCGTACCATAAGCATGCCTTTGGACTTGGGGAGCACTACAATTCTGTGGTTCCTGTA

Coding sequence (CDS)

TTATCTGCATGCAGGAAAGAGATATCACAGTTGCAGGACAAAGAAATTGAACTTAAAAAGACAGCAGCTAGAGGTAGCAAAGCTAAACAAGGAGTGAAGAAGAAACAGGTGGAGGAAGAGATATCTCAACTTTTTGCTAAGCTAAAAAAGAAACACACTGAAGAACTTGCGTCGTTAGGCTATAGTAACAAGAACAGCAACAGTAATGGAAATGAAAAAAATAACCTTGACAATCTAGTGAAGGCCATAGCTGGAGTGTCGATGACAGCTCAATCTGACCATTCAAAGCCCAGCAAAAGCACGAAGAGGAGGGAGAAGAGGGCTCAACAAGAAGCGGAAAGAGACAGAAGAATCGAAGAGGAGCAGAGCAACATTGTGAGTAATAGAATGGTTGAGAATGAGCAGTTGGAAAAGAAGCTTGGACCTCTTGGTTTGACTGTTCACGAAATCAAGCCAGATGGGCATTGCCTCTATCGAGCAGTCGAAAACCAGCTCACCTTCCTTTCCGGTGGTTCGCCTCCATACACATTCCAAGAACTTCGCAAGATGATGGCAGTTTATATGAGAGACTACCATTCACCTAATTACATGCCATTTTTTCTCTCAGACAACACCATAGATGGCAACACCGATTTTGAAAATTACTGCAAGGAAGTCGAGTCAACAGCAGCGTGGGGAGGGCAGCTGGAGCTCGGAGCTTTGACCCATTGCCTTAAAAAACATATCATGATATTTTCAGGATCCTTCCCAGAAGTGGAAATGGGGAAGGAATACAAATCAGAGGGCTTAGTGAAGAGGCTGTCGTACCATAAGCATGCCTTTGGACTTGGGGAGCACTACAATTCTGTGGTTCCTGTA

Protein sequence

LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLGYSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEEEQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQELRKMMAVYMRDYHSPNYMPFFLSDNTIDGNTDFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPEVEMGKEYKSEGLVKRLSYHKHAFGLGEHYNSVVPV
Homology
BLAST of MC09g0644 vs. ExPASy Swiss-Prot
Match: Q9LYC7 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 OS=Arabidopsis thaliana OX=3702 GN=OTU5 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.8e-95
Identity = 190/297 (63.97%), Postives = 237/297 (79.80%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           L+  R+EI QLQ+KE E+KK AA+GSKA+Q  KKKQVE++IS+L  KLK K  +ELAS G
Sbjct: 32  LARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDDISKLSTKLKDKQLKELASQG 91

Query: 61  YSNKNSNS-----NGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERD 120
           +S+ +SN+        +K ++D LV+AIAGVS+TAQ +HSKPSKS KRREKRA++EA+R+
Sbjct: 92  FSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHSKPSKSVKRREKRAKEEADRE 151

Query: 121 RRIEEEQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPY 180
           +RI+EEQS++ S+RMVEN +LEKKL PLGLTV EIKPDGHCLYRAVENQL   SGG+ PY
Sbjct: 152 QRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGHCLYRAVENQLANRSGGASPY 211

Query: 181 TFQELRKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD-----FENYCKEVESTAAWGGQLE 240
           T+Q LR+M A YMR+ H  +++PFFLS+   D N+      FE YC+EVESTAAWG QLE
Sbjct: 212 TYQNLREMAASYMRE-HKTDFLPFFLSETEGDSNSGSAEERFEKYCREVESTAAWGSQLE 271

Query: 241 LGALTHCLKKHIMIFSGSFPEVEMGKEYKS-EGLVKRLSYHKHAFGLGEHYNSVVPV 287
           LGALTHCL+KHI ++SGSFP+VEMGKEY+S +     LSYH+HAFGLGEHYNSVV V
Sbjct: 272 LGALTHCLRKHIKVYSGSFPDVEMGKEYRSGDDSSLMLSYHRHAFGLGEHYNSVVLV 327

BLAST of MC09g0644 vs. ExPASy Swiss-Prot
Match: Q5ZIP6 (Deubiquitinase OTUD6B OS=Gallus gallus OX=9031 GN=OTUD6B PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.9e-37
Identity = 105/285 (36.84%), Postives = 160/285 (56.14%), Query Frame = 0

Query: 5   RKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLGYSNK 64
           R+E  +LQ K   +K    +  K     ++KQ+ EE+++L A+L++KH EEL  L  +  
Sbjct: 23  RREKRELQAKIQGMKNAVPKNDKK----RRKQLAEEVAKLEAELEQKHKEELKQLKEAMP 82

Query: 65  NSNSNGNEKNNLDNLVKAIAGVSMTA---QSDHSKPSKSTKRREKRAQQEAERDRRI-EE 124
                  E+N +D++   +A   +     Q  H + SK+ KRREK+A  E ER+ RI E 
Sbjct: 83  -------EQNKIDSIADGVANFELEGREQQIQHPRISKAQKRREKKAALEKEREERIAEA 142

Query: 125 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 184
           E  N+   R +E+++L   L    L + +I  DGHC+YRA+E+Q   L      +T   L
Sbjct: 143 EIENLTGARHLESQKLASLLAARHLEIKQIPSDGHCMYRAIEDQ---LKDHHNSWTVATL 202

Query: 185 RKMMAVYMRDYHSPNYMPFFLSDNTID--GNTDFENYCKEVESTAAWGGQLELGALTHCL 244
           R   A Y+   H  +++PF  + NT D     +FE YC ++ +TAAWGGQLEL AL+H L
Sbjct: 203 RNQTAKYIHS-HFDDFLPFLTNPNTGDMYSKEEFEKYCDDIANTAAWGGQLELRALSHIL 262

Query: 245 KKHIMIFSGSFPEVEMGKEYKSEGLVKRLSYHKHAFGLGEHYNSV 284
           +  I +     P + +G+EY  + ++  L Y +HA+GLGEHYNSV
Sbjct: 263 QTPIEVVQMDSPSIIVGEEYSGKPII--LVYMRHAYGLGEHYNSV 290

BLAST of MC09g0644 vs. ExPASy Swiss-Prot
Match: Q8N6M0 (Deubiquitinase OTUD6B OS=Homo sapiens OX=9606 GN=OTUD6B PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.2e-35
Identity = 104/282 (36.88%), Postives = 163/282 (57.80%), Query Frame = 0

Query: 5   RKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLGYSNK 64
           RKE  +LQ K   +K    +  K     ++KQ+ E++++L  ++++KH EEL  L  + K
Sbjct: 20  RKEKKELQAKIQGMKNAVPKNDKK----RRKQLTEDVAKLEKEMEQKHREELEQLKLTTK 79

Query: 65  NSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRI-EEEQS 124
                   +N +D++   I+  ++  ++   + SK+ KRREK+A  E ER+ RI E E  
Sbjct: 80  --------ENKIDSVAVNIS--NLVLENQPPRISKAQKRREKKAALEKEREERIAEAEIE 139

Query: 125 NIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQELRKM 184
           N+   R +E+E+L + L    L + +I  DGHC+Y+A+E+Q   L       T   LR  
Sbjct: 140 NLTGARHMESEKLAQILAARQLEIKQIPSDGHCMYKAIEDQ---LKEKDCALTVVALRSQ 199

Query: 185 MAVYMRDYHSPNYMPFFLSDNTIDGNT--DFENYCKEVESTAAWGGQLELGALTHCLKKH 244
            A YM+  H  +++PF  + NT D  T  +F+ YC+++ +TAAWGGQLEL AL+H L+  
Sbjct: 200 TAEYMQS-HVEDFLPFLTNPNTGDMYTPEEFQKYCEDIVNTAAWGGQLELRALSHILQTP 259

Query: 245 IMIFSGSFPEVEMGKEYKSEGLVKRLSYHKHAFGLGEHYNSV 284
           I I     P + +G+EY  + L+  L Y +HA+GLGEHYNSV
Sbjct: 260 IEIIQADSPPIIVGEEYSKKPLI--LVYMRHAYGLGEHYNSV 281

BLAST of MC09g0644 vs. ExPASy Swiss-Prot
Match: Q7ZV00 (Deubiquitinase OTUD6B OS=Danio rerio OX=7955 GN=otud6b PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.7e-35
Identity = 116/288 (40.28%), Postives = 161/288 (55.90%), Query Frame = 0

Query: 5   RKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLGYSNK 64
           RKE   LQ K   +K    +  K     ++KQ+ E+I++L A+L +KH  EL       +
Sbjct: 16  RKEKKDLQAKIQSMKNAVPKNDKK----RRKQLTEDIAKLEAELSQKHENEL-----KLQ 75

Query: 65  NSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSK---PSKSTKRREKRAQQEAERDRRI-EE 124
           N++S     + LD++  A    +   QSD SK    SK+ KRR+K+A  E ER+ RI E 
Sbjct: 76  NTSSVEEVSDALDSMSVA----NHEEQSDPSKQSRTSKAQKRRDKKAALEKEREMRIAEA 135

Query: 125 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 184
           E  N+  +R  E  +L +KL    L + EI  DGHC+YRAVE+QLT           +EL
Sbjct: 136 EVENLSGSRHQEGLKLREKLVERHLQIKEISSDGHCMYRAVEHQLT---ERGLALGLKEL 195

Query: 185 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNT--DFENYCKEVESTAAWGGQLELGALTHCL 244
           R   A YMR  H+ ++MPF  + NT D  T  +FE YC +V  TAAWGGQLEL AL+  L
Sbjct: 196 RDQTAQYMRS-HADDFMPFLTNPNTGDMYTAEEFEKYCSDVADTAAWGGQLELKALSQVL 255

Query: 245 KKHIMIFSGSFPEVEMGKEYKSEGLVKRLSYHKHAFGLGEHYNSVVPV 287
           +  I +     P + +G+EY    +   L Y +HA+GLGEHYNSV P+
Sbjct: 256 QLPIEVIQADSPCITIGEEYDKPKIT--LIYMRHAYGLGEHYNSVEPL 284

BLAST of MC09g0644 vs. ExPASy Swiss-Prot
Match: Q6GM06 (Deubiquitinase OTUD6B OS=Xenopus laevis OX=8355 GN=otud6b PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-33
Identity = 101/284 (35.56%), Postives = 162/284 (57.04%), Query Frame = 0

Query: 5   RKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLGYSNK 64
           RKE  ++Q K   +K +  +  K     ++KQ+ E+I++L A+++ +H EEL +L     
Sbjct: 18  RKEKKEVQAKIQCMKNSVPKNDKK----RRKQMTEDIAKLEAEVEARHKEELEALA---- 77

Query: 65  NSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKP--SKSTKRREKRAQQEAERDRRI-EEE 124
                  E   + ++   +  + + ++    +P  SK+ KRREK+A QE ERD RI E E
Sbjct: 78  ---QKPTEPTQVSSITNGVTSLDLGSEEPVQQPRVSKAQKRREKKAAQEKERDDRIAEAE 137

Query: 125 QSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQELR 184
            +N+   R +E+++L + L    L + +I  DGHC+YRA+E+QL    G S   T   LR
Sbjct: 138 IANLSGARHLESQKLAQILAERELQIRQIPSDGHCMYRAIEHQLN-EQGNS--LTVANLR 197

Query: 185 KMMAVYMRDYHSPNYMPFFLSDNTIDGNT--DFENYCKEVESTAAWGGQLELGALTHCLK 244
              A YM+   + +++PF  + +T D  T  +F+ YC ++ +T AWGGQLEL AL+H LK
Sbjct: 198 SQTADYMQK-RAEDFLPFLTNSSTGDMYTQEEFQKYCTDIVNTPAWGGQLELRALSHILK 257

Query: 245 KHIMIFSGSFPEVEMGKEYKSEGLVKRLSYHKHAFGLGEHYNSV 284
             I +       + +G+EY ++ +   L Y +HA+GLGEHYNSV
Sbjct: 258 TPIEVIQAESLPIVIGEEYSNKPIT--LVYMRHAYGLGEHYNSV 284

BLAST of MC09g0644 vs. NCBI nr
Match: XP_022148608.1 (OTU domain-containing protein 6B [Momordica charantia] >XP_022148617.1 OTU domain-containing protein 6B [Momordica charantia])

HSP 1 Score: 491 bits (1263), Expect = 4.69e-173
Identity = 265/290 (91.38%), Postives = 273/290 (94.14%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q VKKKQVEEEISQL AKLKKKHTEELASLG
Sbjct: 31  LSRHRKEISQLQNKEIELKKAAARGSKAEQKVKKKQVEEEISQLSAKLKKKHTEELASLG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE
Sbjct: 91  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQL FLSGGSPPYTFQEL
Sbjct: 151 EQSNIVSDRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLAFLSGGSPPYTFQEL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD-FENYCKEVESTAAWGGQLELGALTHCLK 240
           RKM+A YMRD HSP+YMPFFLSDNT D   + FENYCKEVESTAAWGGQLELGALTHCL+
Sbjct: 211 RKMVATYMRD-HSPDYMPFFLSDNTDDSLAERFENYCKEVESTAAWGGQLELGALTHCLQ 270

Query: 241 KHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           KHIMIFSGSFP+VEMGKEYKSEGLV    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 KHIMIFSGSFPDVEMGKEYKSEGLVNSSIRLSYHKHAFGLGEHYNSVVPV 319

BLAST of MC09g0644 vs. NCBI nr
Match: XP_023514918.1 (OTU domain-containing protein 6B isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454 bits (1167), Expect = 5.45e-158
Identity = 242/295 (82.03%), Postives = 268/295 (90.85%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           +SACRKEISQLQ+KE+ELKK AARGSKA+Q +KKKQVEEEISQL AKLKKKH EELA LG
Sbjct: 59  VSACRKEISQLQNKEMELKKAAARGSKAEQKLKKKQVEEEISQLSAKLKKKHNEELAFLG 118

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           Y    S+SNGNE++NLDNLVKA+AGVSMTAQSDHSKPSKSTKRREKRAQQEA RDRRIEE
Sbjct: 119 Y----SSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKSTKRREKRAQQEAARDRRIEE 178

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENEQLEKKLGPLGLTV+EIKPDGHCLYRAVENQ+ FLSGGS PY +QEL
Sbjct: 179 EQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSPYNYQEL 238

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLS+N ++GN++      FENYCKEVESTAAWGGQLELGAL
Sbjct: 239 RQMVAAYMRD-HSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQLELGAL 298

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSGSFP+VEMGKEYKS+G++    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 299 THCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSVVPV 348

BLAST of MC09g0644 vs. NCBI nr
Match: XP_023514917.1 (OTU domain-containing protein 6B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454 bits (1167), Expect = 6.50e-158
Identity = 242/295 (82.03%), Postives = 268/295 (90.85%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           +SACRKEISQLQ+KE+ELKK AARGSKA+Q +KKKQVEEEISQL AKLKKKH EELA LG
Sbjct: 64  VSACRKEISQLQNKEMELKKAAARGSKAEQKLKKKQVEEEISQLSAKLKKKHNEELAFLG 123

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           Y    S+SNGNE++NLDNLVKA+AGVSMTAQSDHSKPSKSTKRREKRAQQEA RDRRIEE
Sbjct: 124 Y----SSSNGNERSNLDNLVKAVAGVSMTAQSDHSKPSKSTKRREKRAQQEAARDRRIEE 183

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENEQLEKKLGPLGLTV+EIKPDGHCLYRAVENQ+ FLSGGS PY +QEL
Sbjct: 184 EQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVENQMDFLSGGSSPYNYQEL 243

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLS+N ++GN++      FENYCKEVESTAAWGGQLELGAL
Sbjct: 244 RQMVAAYMRD-HSTDYMPFFLSENVVEGNSNESLSERFENYCKEVESTAAWGGQLELGAL 303

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSGSFP+VEMGKEYKS+G++    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 304 THCLKKHIMIFSGSFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSVVPV 353

BLAST of MC09g0644 vs. NCBI nr
Match: XP_038876728.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 [Benincasa hispida] >XP_038876729.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 [Benincasa hispida])

HSP 1 Score: 451 bits (1159), Expect = 3.32e-157
Identity = 244/295 (82.71%), Postives = 264/295 (89.49%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q VKKKQVEEEISQL   LKKKHTEELAS G
Sbjct: 31  LSRHRKEISQLQNKEIELKKAAARGSKAEQKVKKKQVEEEISQLSVNLKKKHTEELASFG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           +    SNSNGN KNN+DNLVKAIAGVSMTAQSDHSKPSKST+RREKRAQQEAERDRRIEE
Sbjct: 91  F----SNSNGNGKNNIDNLVKAIAGVSMTAQSDHSKPSKSTRRREKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENE LEKKLGPLGLTV+EIKPDGHCLYRAVENQL FLSGGS PY++QEL
Sbjct: 151 EQSNIVSDRMIENEHLEKKLGPLGLTVNEIKPDGHCLYRAVENQLAFLSGGSSPYSYQEL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLSDN ++GN++      FENYCKEVESTAAWGGQLELGAL
Sbjct: 211 REMVAAYMRD-HSTDYMPFFLSDNGVEGNSNGSLAERFENYCKEVESTAAWGGQLELGAL 270

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHI+IFSGSFP+VEMGKEYKS+G V    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 THCLKKHILIFSGSFPDVEMGKEYKSDGKVNSSIRLSYHKHAFGLGEHYNSVVPV 320

BLAST of MC09g0644 vs. NCBI nr
Match: XP_022953804.1 (OTU domain-containing protein 6B-like [Cucurbita moschata])

HSP 1 Score: 450 bits (1158), Expect = 4.71e-157
Identity = 242/295 (82.03%), Postives = 266/295 (90.17%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q +KKKQVEEEISQL AKLKKKHTEELASLG
Sbjct: 31  LSRHRKEISQLQNKEIELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKKKHTEELASLG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           +    S+SNGNEKNN++NLVKAIAGVS TAQSDHSKPSKST+RREKRAQQEAERDRRIEE
Sbjct: 91  F----SDSNGNEKNNIENLVKAIAGVSTTAQSDHSKPSKSTRRREKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENEQLEKKLGPLGLTV+EIKPDGHCLYRAVE+QL  LSGGS PY +QEL
Sbjct: 151 EQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVESQLALLSGGSSPYNYQEL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLSDN ++GN+D      FENYCKE+ESTAAWGGQLELGAL
Sbjct: 211 REMVAAYMRD-HSTDYMPFFLSDNVVEGNSDVSMAERFENYCKEIESTAAWGGQLELGAL 270

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSG FP+VEMGKEYKS+G++    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 THCLKKHIMIFSGCFPDVEMGKEYKSDGVLNSSVRLSYHKHAFGLGEHYNSVVPV 320

BLAST of MC09g0644 vs. ExPASy TrEMBL
Match: A0A6J1D4J8 (OTU domain-containing protein 6B OS=Momordica charantia OX=3673 GN=LOC111017224 PE=4 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 2.27e-173
Identity = 265/290 (91.38%), Postives = 273/290 (94.14%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q VKKKQVEEEISQL AKLKKKHTEELASLG
Sbjct: 31  LSRHRKEISQLQNKEIELKKAAARGSKAEQKVKKKQVEEEISQLSAKLKKKHTEELASLG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE
Sbjct: 91  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQL FLSGGSPPYTFQEL
Sbjct: 151 EQSNIVSDRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLAFLSGGSPPYTFQEL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD-FENYCKEVESTAAWGGQLELGALTHCLK 240
           RKM+A YMRD HSP+YMPFFLSDNT D   + FENYCKEVESTAAWGGQLELGALTHCL+
Sbjct: 211 RKMVATYMRD-HSPDYMPFFLSDNTDDSLAERFENYCKEVESTAAWGGQLELGALTHCLQ 270

Query: 241 KHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           KHIMIFSGSFP+VEMGKEYKSEGLV    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 KHIMIFSGSFPDVEMGKEYKSEGLVNSSIRLSYHKHAFGLGEHYNSVVPV 319

BLAST of MC09g0644 vs. ExPASy TrEMBL
Match: A0A6J1GQT5 (OTU domain-containing protein 6B-like OS=Cucurbita moschata OX=3662 GN=LOC111456222 PE=4 SV=1)

HSP 1 Score: 450 bits (1158), Expect = 2.28e-157
Identity = 242/295 (82.03%), Postives = 266/295 (90.17%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q +KKKQVEEEISQL AKLKKKHTEELASLG
Sbjct: 31  LSRHRKEISQLQNKEIELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKKKHTEELASLG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           +    S+SNGNEKNN++NLVKAIAGVS TAQSDHSKPSKST+RREKRAQQEAERDRRIEE
Sbjct: 91  F----SDSNGNEKNNIENLVKAIAGVSTTAQSDHSKPSKSTRRREKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENEQLEKKLGPLGLTV+EIKPDGHCLYRAVE+QL  LSGGS PY +QEL
Sbjct: 151 EQSNIVSDRMIENEQLEKKLGPLGLTVNEIKPDGHCLYRAVESQLALLSGGSSPYNYQEL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLSDN ++GN+D      FENYCKE+ESTAAWGGQLELGAL
Sbjct: 211 REMVAAYMRD-HSTDYMPFFLSDNVVEGNSDVSMAERFENYCKEIESTAAWGGQLELGAL 270

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSG FP+VEMGKEYKS+G++    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 THCLKKHIMIFSGCFPDVEMGKEYKSDGVLNSSVRLSYHKHAFGLGEHYNSVVPV 320

BLAST of MC09g0644 vs. ExPASy TrEMBL
Match: A0A1S3BRX4 (OTU domain-containing protein 6B OS=Cucumis melo OX=3656 GN=LOC103492860 PE=4 SV=1)

HSP 1 Score: 449 bits (1154), Expect = 8.95e-157
Identity = 245/295 (83.05%), Postives = 266/295 (90.17%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q VKKKQVEEEISQL  KLKKKH EELASLG
Sbjct: 31  LSRHRKEISQLQNKEIELKKAAARGSKAEQKVKKKQVEEEISQLSGKLKKKHAEELASLG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           +    SNSNGNEKN +DNLVKAIAGVSMTAQSDHSKPSKST+R+EKRAQQEAERDRRIEE
Sbjct: 91  F----SNSNGNEKN-IDNLVKAIAGVSMTAQSDHSKPSKSTRRKEKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENE LEKKLGPLGLTV+EIKPDGHCLYRAVENQL +LSGGS PY +Q+L
Sbjct: 151 EQSNIVSDRMIENEHLEKKLGPLGLTVNEIKPDGHCLYRAVENQLAYLSGGSSPYNYQQL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLSDN I+GN++      FENYCKEVESTAAWGGQLELGAL
Sbjct: 211 REMVAAYMRD-HSTDYMPFFLSDNGIEGNSNSSLAERFENYCKEVESTAAWGGQLELGAL 270

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSGSFP+VEMGKEYKSEG+V+   RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 THCLKKHIMIFSGSFPDVEMGKEYKSEGVVESSIRLSYHKHAFGLGEHYNSVVPV 319

BLAST of MC09g0644 vs. ExPASy TrEMBL
Match: A0A5D3D3S7 (OTU domain-containing protein 6B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00070 PE=4 SV=1)

HSP 1 Score: 449 bits (1154), Expect = 1.47e-156
Identity = 245/295 (83.05%), Postives = 266/295 (90.17%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS  RKEISQLQ+KEIELKK AARGSKA+Q VKKKQVEEEISQL  KLKKKH EELASLG
Sbjct: 45  LSRHRKEISQLQNKEIELKKAAARGSKAEQKVKKKQVEEEISQLSGKLKKKHAEELASLG 104

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           +    SNSNGNEKN +DNLVKAIAGVSMTAQSDHSKPSKST+R+EKRAQQEAERDRRIEE
Sbjct: 105 F----SNSNGNEKN-IDNLVKAIAGVSMTAQSDHSKPSKSTRRKEKRAQQEAERDRRIEE 164

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RM+ENE LEKKLGPLGLTV+EIKPDGHCLYRAVENQL +LSGGS PY +Q+L
Sbjct: 165 EQSNIVSDRMIENEHLEKKLGPLGLTVNEIKPDGHCLYRAVENQLAYLSGGSSPYNYQQL 224

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLSDN I+GN++      FENYCKEVESTAAWGGQLELGAL
Sbjct: 225 REMVAAYMRD-HSTDYMPFFLSDNGIEGNSNSSLAERFENYCKEVESTAAWGGQLELGAL 284

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSGSFP+VEMGKEYKSEG+V+   RLSYHKHAFGLGEHYNSVVPV
Sbjct: 285 THCLKKHIMIFSGSFPDVEMGKEYKSEGVVESSIRLSYHKHAFGLGEHYNSVVPV 333

BLAST of MC09g0644 vs. ExPASy TrEMBL
Match: A0A6J1JQT7 (OTU domain-containing protein 6B-like OS=Cucurbita maxima OX=3661 GN=LOC111488073 PE=4 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 7.59e-156
Identity = 241/295 (81.69%), Postives = 264/295 (89.49%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           LS   KEISQLQ+KEIELKK AARGSKA+Q +KKKQVEEEISQL AKLKKKHTEELASLG
Sbjct: 31  LSRHGKEISQLQNKEIELKKAAARGSKAEQKMKKKQVEEEISQLSAKLKKKHTEELASLG 90

Query: 61  YSNKNSNSNGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERDRRIEE 120
           +    S+SNGNEKNN++NLVKAIAGVS T QSDHSKPSKST+RREKRAQQEAERDRRIEE
Sbjct: 91  F----SDSNGNEKNNIENLVKAIAGVSTTGQSDHSKPSKSTRRREKRAQQEAERDRRIEE 150

Query: 121 EQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQEL 180
           EQSNIVS+RMVENEQLEKKLGPLGLTV+EIKPDGHCLYRAVE+QL  LSGGS PY +QEL
Sbjct: 151 EQSNIVSDRMVENEQLEKKLGPLGLTVNEIKPDGHCLYRAVESQLALLSGGSSPYNYQEL 210

Query: 181 RKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD------FENYCKEVESTAAWGGQLELGAL 240
           R+M+A YMRD HS +YMPFFLSDN ++GN+D      FENYCKE+ESTAAWGGQLELGAL
Sbjct: 211 REMVAAYMRD-HSTDYMPFFLSDNVVEGNSDVSMAARFENYCKEIESTAAWGGQLELGAL 270

Query: 241 THCLKKHIMIFSGSFPEVEMGKEYKSEGLVK---RLSYHKHAFGLGEHYNSVVPV 286
           THCLKKHIMIFSG FP+VEMGKEYKS+G++    RLSYHKHAFGLGEHYNSVVPV
Sbjct: 271 THCLKKHIMIFSGCFPDVEMGKEYKSDGVLNSSIRLSYHKHAFGLGEHYNSVVPV 320

BLAST of MC09g0644 vs. TAIR 10
Match: AT3G62940.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-96
Identity = 190/297 (63.97%), Postives = 237/297 (79.80%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           L+  R+EI QLQ+KE E+KK AA+GSKA+Q  KKKQVE++IS+L  KLK K  +ELAS G
Sbjct: 16  LARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDDISKLSTKLKDKQLKELASQG 75

Query: 61  YSNKNSNS-----NGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERD 120
           +S+ +SN+        +K ++D LV+AIAGVS+TAQ +HSKPSKS KRREKRA++EA+R+
Sbjct: 76  FSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHSKPSKSVKRREKRAKEEADRE 135

Query: 121 RRIEEEQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPY 180
           +RI+EEQS++ S+RMVEN +LEKKL PLGLTV EIKPDGHCLYRAVENQL   SGG+ PY
Sbjct: 136 QRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGHCLYRAVENQLANRSGGASPY 195

Query: 181 TFQELRKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD-----FENYCKEVESTAAWGGQLE 240
           T+Q LR+M A YMR+ H  +++PFFLS+   D N+      FE YC+EVESTAAWG QLE
Sbjct: 196 TYQNLREMAASYMRE-HKTDFLPFFLSETEGDSNSGSAEERFEKYCREVESTAAWGSQLE 255

Query: 241 LGALTHCLKKHIMIFSGSFPEVEMGKEYKS-EGLVKRLSYHKHAFGLGEHYNSVVPV 287
           LGALTHCL+KHI ++SGSFP+VEMGKEY+S +     LSYH+HAFGLGEHYNSVV V
Sbjct: 256 LGALTHCLRKHIKVYSGSFPDVEMGKEYRSGDDSSLMLSYHRHAFGLGEHYNSVVLV 311

BLAST of MC09g0644 vs. TAIR 10
Match: AT3G62940.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-96
Identity = 190/297 (63.97%), Postives = 237/297 (79.80%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           L+  R+EI QLQ+KE E+KK AA+GSKA+Q  KKKQVE++IS+L  KLK K  +ELAS G
Sbjct: 32  LARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDDISKLSTKLKDKQLKELASQG 91

Query: 61  YSNKNSNS-----NGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERD 120
           +S+ +SN+        +K ++D LV+AIAGVS+TAQ +HSKPSKS KRREKRA++EA+R+
Sbjct: 92  FSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHSKPSKSVKRREKRAKEEADRE 151

Query: 121 RRIEEEQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPY 180
           +RI+EEQS++ S+RMVEN +LEKKL PLGLTV EIKPDGHCLYRAVENQL   SGG+ PY
Sbjct: 152 QRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGHCLYRAVENQLANRSGGASPY 211

Query: 181 TFQELRKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD-----FENYCKEVESTAAWGGQLE 240
           T+Q LR+M A YMR+ H  +++PFFLS+   D N+      FE YC+EVESTAAWG QLE
Sbjct: 212 TYQNLREMAASYMRE-HKTDFLPFFLSETEGDSNSGSAEERFEKYCREVESTAAWGSQLE 271

Query: 241 LGALTHCLKKHIMIFSGSFPEVEMGKEYKS-EGLVKRLSYHKHAFGLGEHYNSVVPV 287
           LGALTHCL+KHI ++SGSFP+VEMGKEY+S +     LSYH+HAFGLGEHYNSVV V
Sbjct: 272 LGALTHCLRKHIKVYSGSFPDVEMGKEYRSGDDSSLMLSYHRHAFGLGEHYNSVVLV 327

BLAST of MC09g0644 vs. TAIR 10
Match: AT3G62940.3 (Cysteine proteinases superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-96
Identity = 190/297 (63.97%), Postives = 237/297 (79.80%), Query Frame = 0

Query: 1   LSACRKEISQLQDKEIELKKTAARGSKAKQGVKKKQVEEEISQLFAKLKKKHTEELASLG 60
           L+  R+EI QLQ+KE E+KK AA+GSKA+Q  KKKQVE++IS+L  KLK K  +ELAS G
Sbjct: 32  LARHRQEIKQLQNKETEMKKAAAKGSKAEQKAKKKQVEDDISKLSTKLKDKQLKELASQG 91

Query: 61  YSNKNSNS-----NGNEKNNLDNLVKAIAGVSMTAQSDHSKPSKSTKRREKRAQQEAERD 120
           +S+ +SN+        +K ++D LV+AIAGVS+TAQ +HSKPSKS KRREKRA++EA+R+
Sbjct: 92  FSSSSSNNIAKDETTEKKGDIDTLVRAIAGVSVTAQQEHSKPSKSVKRREKRAKEEADRE 151

Query: 121 RRIEEEQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPY 180
           +RI+EEQS++ S+RMVEN +LEKKL PLGLTV EIKPDGHCLYRAVENQL   SGG+ PY
Sbjct: 152 QRIKEEQSHVKSDRMVENAKLEKKLKPLGLTVSEIKPDGHCLYRAVENQLANRSGGASPY 211

Query: 181 TFQELRKMMAVYMRDYHSPNYMPFFLSDNTIDGNTD-----FENYCKEVESTAAWGGQLE 240
           T+Q LR+M A YMR+ H  +++PFFLS+   D N+      FE YC+EVESTAAWG QLE
Sbjct: 212 TYQNLREMAASYMRE-HKTDFLPFFLSETEGDSNSGSAEERFEKYCREVESTAAWGSQLE 271

Query: 241 LGALTHCLKKHIMIFSGSFPEVEMGKEYKS-EGLVKRLSYHKHAFGLGEHYNSVVPV 287
           LGALTHCL+KHI ++SGSFP+VEMGKEY+S +     LSYH+HAFGLGEHYNSVV V
Sbjct: 272 LGALTHCLRKHIKVYSGSFPDVEMGKEYRSGDDSSLMLSYHRHAFGLGEHYNSVVLV 327

BLAST of MC09g0644 vs. TAIR 10
Match: AT5G67170.2 (SEC-C motif-containing protein / OTU-like cysteine protease family protein )

HSP 1 Score: 66.6 bits (161), Expect = 3.6e-11
Identity = 43/149 (28.86%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 135 QLEKKLGPLGLTVHEIKPDGHCLYRAVENQLTFLSGGSPPYTFQELRKMMAVYMRDYHSP 194
           Q   +L  LGL + ++  DG+C +RA+ +Q   L G    +   + R M+ +Y+   +  
Sbjct: 26  QFRAQLDALGLKIIQVTADGNCFFRAIADQ---LEGNEDEH--NKYRNMIVLYIVK-NRE 85

Query: 195 NYMPFFLSDNTIDGNTDFENYCKEVESTAAWGGQLELGALTHCLKKHIMIFSGSFPEVEM 254
            + PF      I+ +  FE+YCK ++    W G +EL A +   + +I I     P   +
Sbjct: 86  MFEPF------IEDDVPFEDYCKTMDDDGTWAGNMELQAASLVTRSNICIHRNMSPRWYI 145

Query: 255 GKEYKSEGLVKRLSYHKHAFGLGEHYNSV 284
                +   +  LSYH      GEHYNSV
Sbjct: 146 RNFEDTRTRMIHLSYHD-----GEHYNSV 157

BLAST of MC09g0644 vs. TAIR 10
Match: AT5G67170.1 (SEC-C motif-containing protein / OTU-like cysteine protease family protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.6e-09
Identity = 47/178 (26.40%), Postives = 83/178 (46.63%), Query Frame = 0

Query: 106 KRAQQEAERDRRIEEEQSNIVSNRMVENEQLEKKLGPLGLTVHEIKPDGHCLYRAVENQL 165
           K  QQ+++  ++  ++Q      +  +  Q   +L  LGL + ++  DG+C +RA+ +Q 
Sbjct: 3   KTKQQKSKPKKQPHQKQ-----GKDCDLSQFRAQLDALGLKIIQVTADGNCFFRAIADQ- 62

Query: 166 TFLSGGSPPYTFQELRKMMAVYMRDYHSPNYMPFFLSDNTIDGNTDFENYCKEVESTAAW 225
             L G    +   + R M+ +Y+   +   + PF      I+ +  FE+YCK ++    W
Sbjct: 63  --LEGNEDEH--NKYRNMIVLYIVK-NREMFEPF------IEDDVPFEDYCKTMDDDGTW 122

Query: 226 GGQLELGALTHCLKKHIMIFSGSFPEVEMGKEYKSEGLVKRLSYHKHAFGLGEHYNSV 284
            G +EL A +   + +I I     P   +     +   +  LSYH      GEHYNSV
Sbjct: 123 AGNMELQAASLVTRSNICIHRNMSPRWYIRNFEDTRTRMIHLSYHD-----GEHYNSV 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYC71.8e-9563.97OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 OS=Arabidopsis thalian... [more]
Q5ZIP64.9e-3736.84Deubiquitinase OTUD6B OS=Gallus gallus OX=9031 GN=OTUD6B PE=2 SV=1[more]
Q8N6M01.2e-3536.88Deubiquitinase OTUD6B OS=Homo sapiens OX=9606 GN=OTUD6B PE=1 SV=1[more]
Q7ZV002.7e-3540.28Deubiquitinase OTUD6B OS=Danio rerio OX=7955 GN=otud6b PE=2 SV=1[more]
Q6GM063.3e-3335.56Deubiquitinase OTUD6B OS=Xenopus laevis OX=8355 GN=otud6b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022148608.14.69e-17391.38OTU domain-containing protein 6B [Momordica charantia] >XP_022148617.1 OTU domai... [more]
XP_023514918.15.45e-15882.03OTU domain-containing protein 6B isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023514917.16.50e-15882.03OTU domain-containing protein 6B isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_038876728.13.32e-15782.71OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 5 [Benincasa hispida] >X... [more]
XP_022953804.14.71e-15782.03OTU domain-containing protein 6B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1D4J82.27e-17391.38OTU domain-containing protein 6B OS=Momordica charantia OX=3673 GN=LOC111017224 ... [more]
A0A6J1GQT52.28e-15782.03OTU domain-containing protein 6B-like OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A1S3BRX48.95e-15783.05OTU domain-containing protein 6B OS=Cucumis melo OX=3656 GN=LOC103492860 PE=4 SV... [more]
A0A5D3D3S71.47e-15683.05OTU domain-containing protein 6B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1JQT77.59e-15681.69OTU domain-containing protein 6B-like OS=Cucurbita maxima OX=3661 GN=LOC11148807... [more]
Match NameE-valueIdentityDescription
AT3G62940.11.3e-9663.97Cysteine proteinases superfamily protein [more]
AT3G62940.21.3e-9663.97Cysteine proteinases superfamily protein [more]
AT3G62940.31.3e-9663.97Cysteine proteinases superfamily protein [more]
AT5G67170.23.6e-1128.86SEC-C motif-containing protein / OTU-like cysteine protease family protein [more]
AT5G67170.12.6e-0926.40SEC-C motif-containing protein / OTU-like cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 34..54
NoneNo IPR availableCOILSCoilCoilcoord: 102..122
NoneNo IPR availableCOILSCoilCoilcoord: 1..21
NoneNo IPR availableGENE3D3.90.70.80coord: 131..286
e-value: 1.3E-30
score: 108.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 88..121
NoneNo IPR availablePANTHERPTHR12419OTU DOMAIN CONTAINING PROTEINcoord: 5..285
NoneNo IPR availablePANTHERPTHR12419:SF10DEUBIQUITINASE OTUD6Bcoord: 5..285
IPR003323OTU domainPFAMPF02338OTUcoord: 151..280
e-value: 7.1E-28
score: 97.7
IPR003323OTU domainPROSITEPS50802OTUcoord: 145..286
score: 23.282404
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 111..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0644.1MC09g0644.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
molecular_function GO:0004843 thiol-dependent deubiquitinase