MC09g0634 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0634
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmetal tolerance protein C1
LocationMC09: 5772126 .. 5776646 (-)
RNA-Seq ExpressionMC09g0634
SyntenyMC09g0634
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAAAACTGAGTATTATTAAAATTAATACACCTGAATTAAGGTTAAAAACATCTTTTTCCAAATTAAAAAAAAAAAGAAAAAAAAACTGCATGATCGGTGCAGGGAGAAGGTTCTCAAATCTCAACTGGGGAATCTTTAATTTTGTACAGAATCGGTGCTTCATTGCCGTTCTACGCAGTGTTTGTTCTCTCTTCACCTTCAACTCTCGCGATTCAGAAATCAAACACCAAACTTCATAAATCTCTTAGTTTCTGTTAATCAAAAAATCCACCACTTCCCCATCAAACTCCATTAAAATGAGGTACAGATTCTACCGTCTCAACCCAATACTTCAATCCTTCTACACCAGGCTTTCCTCCGCAACCCACCAGGAATTTCATTCAATTCCGCCAATCCAATCCCTCAATCTTCAACCCCAGAACTCACTATTGGGAATCTACGACGATCCGAAGAACAGAATCTCCAGACGGTGGCATTTGGGCCACGCCCACCGCCACGACGACCACCATCGATTCGGCGAAGAGGGCGAGAGTATATTCAAGCTGGGTCTCGGCGCTGACATTGCCTTGGCTGCGGGGAAGGCTGTGACGGGTTATTTGTCAGGAAGCACAGCCATCATCGCCGATGCGGCTCATTCAGTGTCTGATGTGGTAATTTTTATCTGAAACTTTTGATTTCTTAAGTTGTCTTCTTGGTTGAGTTCTTGAAGCTGCTGCCCTGATCCCTCAATCGAGTTCAGCTTTTAACAACATTTGAAATTCTGCTCATTCTAAAGATGAATTGAATAGATTGTGCCTTTTGTTTTTCTACTTATTGTGTTTGGTTTCCTAATTTTTTCTTGAATCCAACGATATTTGACAATTACTTGCCTTATTCTTGTACGCTACAGGTTCTTAGTGGCATCGCTTTATGGTCGTTTAAAGCTGCAAAAGCTCCTAAAGACAAGGAGCACCCATATGGTCACTCTTGATTTGTACCTACATGCTACTCCATTTTTCTCTCCCTATATTATGAATTCTCAGCTTAATGGTTTTGTATCATGTAGAAAAACATTTTTTTTTCTTTAAGTTATCAACTCATGTTGATTATACATCTAGTTTACAAATCCGTGAGAAAAATATGACCATTGACAACAAATGTTAAACTATCTCTTTAAACTATCCTTTGGGTTTATGTTCATTTCAATTGTAAGACTTATGAGGCTCAGCACTTTAAATTTGAACCAAGGAGAATGATGCGCTTTTATATGACATTTAGAAAATCATTTGCGTGTGTTTACTACTATCTTCATATTGAAATATTTCTGATTCTTGGGTTTATAAAACATATGAATCATGAGAGTGGACTTTTTAATGATTCGTATTGTGTTCAATCCTGATCGAACTCATATTTTAAACAGATTTAGTTGTTAATAGTTAATACAGGGAATTTCCTTACCATCTAGTATATACTTGGTATACCTAAAAAGCTAGGTGTTCAGGATGGTTTTACTCCTACTCTGCATTTCCAGTTAAAAATAACATTGTCAAAATGATATTGTTTGTCTGAGATGCTAATGTCGTGCTTTCTCTCGCTTTGAAATACTATTATTTTACAACTCCTTCTCTGCCATATATTCTCAGGACATGGTAAATTTGAGACTCTGGGAGCCCTTGGAATCTCTTGCATGCTTTTAGCAACTGCTGGTGGCATTGCTTGGCATGCTTCAGAACTTTTACTGGTATGCTTGATTATCCATTTTGCTCCTAATTGAACTCAGAGTAATACAGATGTTATCTGAATCTTCAGAATCTAATCCCTCTTCCATTTTCACATAATCCTGGTGTTATTAGGATTTGAGGAATTTCACATTGTAACTTGTGGAAATTTTGTTTACATGGTAAAAGTTTTGGATCCGAATTTCGTGTCGTAGCCAACTAAGCCTCCTTTTTTTCCTTGTAAGCAGGGCTTGCTGTCAGCTGCTCCTGAAATAGTTAATCAGCCGTTGGGACATGATGATTTGCATAACCATAGCCATGGTGGACATCATCACGGGATCGACATGGACCATCCTATTCTAGCCTTGAATATGACTCTTGTATCAATATCTATCAAAGAAGGGTAAGGGTACTTGCCCGTGTTTCACTCTGTTAAGAACTGGAGCACTTTGAAATGCTCGTTCATTGACCATGTTTCCCTGTTATGCTACCTTATTAGTTTATAGTTTAGAAAACCTCAAGTTAATTAGTAAGCATCTTAGATGAGTGCTGTATCCAATCTTGTACTTTTGATAGTCATAATGCTTCCCATTCAGCCGAACTTTGAAAATATGCTAATGTCTTGCATCCTTAACTAGACAATAGAATGGTCATTATTTTGGCAGTTTGCTGCCACACTATGATAATTGGTTCTCGTCTTGACCCCTGATAGGCTTTATTGGATAACAAAGCGAGCTGGGGAAAAACAAGGTAGCGGACTCATGAAAGCAAATGCATGGCATCATCGGGCTGATGCTATATCATCAGTAGTTGCGCTCATTGGCGTTGGTGAGGAACTCTGGTCCTTGTAGTCAGCATTAAGCTATCTAGTAGTCTAGTCTGCATACTTCCAGCTCCTCTGGCAGGGATCAATGATTTTTATTTCAGGAGGTTCTATTCTCGGGGTGAAGTTTCTAGATCCCTTAGCTGGACTCGTTGTCTCGGGCATGATTCTGAAGGCTGGACTTGGAAGTGGACACCAAAGGTGTGTAATACATTTTAGTCAGAAACACGGGTCACGAAAACAATACTCAATTTTCATTTTTATTTCAGATCTGCATCACATTGATTATTTTTTTTAGGAATTGCTAATGTGATGTCTAATAATATGACTGTAACAAAACATTGAATAGTTCCAAGTAAAACTGCAGTGGGATAATGAATTTGTTTCCAGAATTTATTATTGTCCATAAACTTCAATCCACTGTTTCAGTTACTAGTTGAAGAGAATTTATGCATAATCGTTTTCTTGGTTTCTATATTTCTAATGTTCTTCGTAATAAATTTTAGCATCTTGGAATTAGTGGATGCTGCAATTCCAGCAGATCAGATAGAGCCTGTCAGACGAACAATACTGCAAGTTGAGGGTGTCATGGTAATTATTATAAGTTTTGTGTTATCTGTTAACTTTAAAGATGATGTGTAAAATAGAATTTTGACTTCTGGGTGTCTGGTTATTGGGAGAGGGGAGTTGAAAGTTTATGGTGTTGGCCTCCATTTCCATGCTTTCCTGTCTTGTTCATTATGAAAACAAGTCTCTGTGATCCTGCTGATGTCGAAAAACCATCTTTAGGGCTGCCATCGCCTTAGAGGAAGGAGAGCTGGTTCTTCTTTGTATCTCGATGTGCATATTGAGGTTCGACCTCTGATTGAATTACAAGTATTTCACTACTAAATGTGGTTGATGGACATCGGTTTTATACTTTGCTTTTGCTTGCAGGTTGATCCTTTTCTGAGTGTCAGTGCTGCTCATTGTATAGGTGAAAATGCTCGTCGAAAGATTCACACATCACATCCTGAAGTATCTGAAGTTTTTATACATATAGGTACATTGCTCAGTTCATTTTCCTTGCAGATCATCACACATGTTCTAGTTGAACGATTTCCAGAATCGGAACGTTTCCTTTTTCCTCATCACTAATAATGATCACCTGTAGATCCCTCAATTTCACATTTCCAACACAGTTTATCGGACTGGCAAGAAGGCTCGATCGGAACTTTAAATCAGAGTACAGATACCCTGCTGAACGAGAAGAAAATTGAATCCATGATTTCCAGAATCATCCAGTCACAATTTCCTGAGGTGAATTCCGTAAACGTTCTGAGCTGAAACTTTTGTTCATTTGACTGATTAGCATATTTCTCTTTGAACGTGTTTCAGAAAATGGTTGTTGAACGCATTACACCCCACTTGTTGCAAGGCAAGATTCTGCTAGAAATCGAGGTCTCCATGCCTCCCGACTTGCTAGTTCGGTAAAACTCTCAAACCCGACAAACCTTAAACTGCTTAGTTTGGTCTGTCGTTGTTGATTCTCCAAGCAAACGTGTTGTGTTGTGTTGTGTCCTCAGAAATGCAATGGAAGTAGCAAGAGTAGCAGAGATGCAGATCCTGAAGGCAGCCTCAAACATAGTTCATGTTAGCATTCAGCTGCGTTTGGGGCATAGAATTCCACAGCTAAGCCACCAGGCAAAATTTTAAAATGCGACTTTTTATTAGACTTCTCATGTTTCATAGCTAAATTTTTATTGGGGGTGACATCATTTGACCCCATTACCTATAGATTTTTTCTTGGAATCTCTCCTATGAGAAAGAGAGTTTGGTTGAAGGTCTCAAAAAGTAGCTCAAAATTTTATGTTATGGTAAATATTTGATTAACAACTAATCAAATTTAATAAAAATGTAAAGAAAATATTTGGTAAATTCAATAAGTTTCTATTTGATTACAGAATTTCAAAAATTCTCAATTTTGATAATTATCTTTTAGAAAATAGTTTTAAAAGAT

mRNA sequence

GTGAAAACTGAGTATTATTAAAATTAATACACCTGAATTAAGGTTAAAAACATCTTTTTCCAAATTAAAAAAAAAAAGAAAAAAAAACTGCATGATCGGTGCAGGGAGAAGGTTCTCAAATCTCAACTGGGGAATCTTTAATTTTGTACAGAATCGGTGCTTCATTGCCGTTCTACGCAGTGTTTGTTCTCTCTTCACCTTCAACTCTCGCGATTCAGAAATCAAACACCAAACTTCATAAATCTCTTAGTTTCTGTTAATCAAAAAATCCACCACTTCCCCATCAAACTCCATTAAAATGAGGTACAGATTCTACCGTCTCAACCCAATACTTCAATCCTTCTACACCAGGCTTTCCTCCGCAACCCACCAGGAATTTCATTCAATTCCGCCAATCCAATCCCTCAATCTTCAACCCCAGAACTCACTATTGGGAATCTACGACGATCCGAAGAACAGAATCTCCAGACGGTGGCATTTGGGCCACGCCCACCGCCACGACGACCACCATCGATTCGGCGAAGAGGGCGAGAGTATATTCAAGCTGGGTCTCGGCGCTGACATTGCCTTGGCTGCGGGGAAGGCTGTGACGGGTTATTTGTCAGGAAGCACAGCCATCATCGCCGATGCGGCTCATTCAGTGTCTGATGTGGTTCTTAGTGGCATCGCTTTATGGTCGTTTAAAGCTGCAAAAGCTCCTAAAGACAAGGAGCACCCATATGGACATGGTAAATTTGAGACTCTGGGAGCCCTTGGAATCTCTTGCATGCTTTTAGCAACTGCTGGTGGCATTGCTTGGCATGCTTCAGAACTTTTACTGGGCTTGCTGTCAGCTGCTCCTGAAATAGTTAATCAGCCGTTGGGACATGATGATTTGCATAACCATAGCCATGGTGGACATCATCACGGGATCGACATGGACCATCCTATTCTAGCCTTGAATATGACTCTTGTATCAATATCTATCAAAGAAGGGCTTTATTGGATAACAAAGCGAGCTGGGGAAAAACAAGGTAGCGGACTCATGAAAGCAAATGCATGGCATCATCGGGCTGATGCTATATCATCAGTAGTTGCGCTCATTGGCGTTGGAGGTTCTATTCTCGGGGTGAAGTTTCTAGATCCCTTAGCTGGACTCGTTGTCTCGGGCATGATTCTGAAGGCTGGACTTGGAAGTGGACACCAAAGCATCTTGGAATTAGTGGATGCTGCAATTCCAGCAGATCAGATAGAGCCTGTCAGACGAACAATACTGCAAGTTGAGGGTGTCATGGGCTGCCATCGCCTTAGAGGAAGGAGAGCTGGTTCTTCTTTGTATCTCGATGTGCATATTGAGGTTGATCCTTTTCTGAGTGTCAGTGCTGCTCATTGTATAGGTGAAAATGCTCGTCGAAAGATTCACACATCACATCCTGAAGTATCTGAAGTTTTTATACATATAGATCCCTCAATTTCACATTTCCAACACAGTTTATCGGACTGGCAAGAAGGCTCGATCGGAACTTTAAATCAGAGTACAGATACCCTGCTGAACGAGAAGAAAATTGAATCCATGATTTCCAGAATCATCCAGTCACAATTTCCTGAGAAAATGGTTGTTGAACGCATTACACCCCACTTGTTGCAAGGCAAGATTCTGCTAGAAATCGAGGTCTCCATGCCTCCCGACTTGCTAGTTCGAAATGCAATGGAAGTAGCAAGAGTAGCAGAGATGCAGATCCTGAAGGCAGCCTCAAACATAGTTCATGTTAGCATTCAGCTGCGTTTGGGGCATAGAATTCCACAGCTAAGCCACCAGGCAAAATTTTAAAATGCGACTTTTTATTAGACTTCTCATGTTTCATAGCTAAATTTTTATTGGGGGTGACATCATTTGACCCCATTACCTATAGATTTTTTCTTGGAATCTCTCCTATGAGAAAGAGAGTTTGGTTGAAGGTCTCAAAAAGTAGCTCAAAATTTTATGTTATGGTAAATATTTGATTAACAACTAATCAAATTTAATAAAAATGTAAAGAAAATATTTGGTAAATTCAATAAGTTTCTATTTGATTACAGAATTTCAAAAATTCTCAATTTTGATAATTATCTTTTAGAAAATAGTTTTAAAAGAT

Coding sequence (CDS)

ATGAGGTACAGATTCTACCGTCTCAACCCAATACTTCAATCCTTCTACACCAGGCTTTCCTCCGCAACCCACCAGGAATTTCATTCAATTCCGCCAATCCAATCCCTCAATCTTCAACCCCAGAACTCACTATTGGGAATCTACGACGATCCGAAGAACAGAATCTCCAGACGGTGGCATTTGGGCCACGCCCACCGCCACGACGACCACCATCGATTCGGCGAAGAGGGCGAGAGTATATTCAAGCTGGGTCTCGGCGCTGACATTGCCTTGGCTGCGGGGAAGGCTGTGACGGGTTATTTGTCAGGAAGCACAGCCATCATCGCCGATGCGGCTCATTCAGTGTCTGATGTGGTTCTTAGTGGCATCGCTTTATGGTCGTTTAAAGCTGCAAAAGCTCCTAAAGACAAGGAGCACCCATATGGACATGGTAAATTTGAGACTCTGGGAGCCCTTGGAATCTCTTGCATGCTTTTAGCAACTGCTGGTGGCATTGCTTGGCATGCTTCAGAACTTTTACTGGGCTTGCTGTCAGCTGCTCCTGAAATAGTTAATCAGCCGTTGGGACATGATGATTTGCATAACCATAGCCATGGTGGACATCATCACGGGATCGACATGGACCATCCTATTCTAGCCTTGAATATGACTCTTGTATCAATATCTATCAAAGAAGGGCTTTATTGGATAACAAAGCGAGCTGGGGAAAAACAAGGTAGCGGACTCATGAAAGCAAATGCATGGCATCATCGGGCTGATGCTATATCATCAGTAGTTGCGCTCATTGGCGTTGGAGGTTCTATTCTCGGGGTGAAGTTTCTAGATCCCTTAGCTGGACTCGTTGTCTCGGGCATGATTCTGAAGGCTGGACTTGGAAGTGGACACCAAAGCATCTTGGAATTAGTGGATGCTGCAATTCCAGCAGATCAGATAGAGCCTGTCAGACGAACAATACTGCAAGTTGAGGGTGTCATGGGCTGCCATCGCCTTAGAGGAAGGAGAGCTGGTTCTTCTTTGTATCTCGATGTGCATATTGAGGTTGATCCTTTTCTGAGTGTCAGTGCTGCTCATTGTATAGGTGAAAATGCTCGTCGAAAGATTCACACATCACATCCTGAAGTATCTGAAGTTTTTATACATATAGATCCCTCAATTTCACATTTCCAACACAGTTTATCGGACTGGCAAGAAGGCTCGATCGGAACTTTAAATCAGAGTACAGATACCCTGCTGAACGAGAAGAAAATTGAATCCATGATTTCCAGAATCATCCAGTCACAATTTCCTGAGAAAATGGTTGTTGAACGCATTACACCCCACTTGTTGCAAGGCAAGATTCTGCTAGAAATCGAGGTCTCCATGCCTCCCGACTTGCTAGTTCGAAATGCAATGGAAGTAGCAAGAGTAGCAGAGATGCAGATCCTGAAGGCAGCCTCAAACATAGTTCATGTTAGCATTCAGCTGCGTTTGGGGCATAGAATTCCACAGCTAAGCCACCAGGCAAAATTTTAA

Protein sequence

MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWHLGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAAPEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASNIVHVSIQLRLGHRIPQLSHQAKF
Homology
BLAST of MC09g0634 vs. ExPASy Swiss-Prot
Match: Q8L725 (Metal tolerance protein C1 OS=Arabidopsis thaliana OX=3702 GN=MTPC1 PE=2 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 4.1e-156
Identity = 291/440 (66.14%), Postives = 337/440 (76.59%), Query Frame = 0

Query: 51  PKNRISRRWHLGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIAD 110
           P    SRRWH GH   H  + + GEEGE IF+LGL ADI L+  KA+TGYL GSTAIIAD
Sbjct: 47  PSFDFSRRWHFGHPDHHQQYQKPGEEGEKIFRLGLTADIGLSVAKALTGYLCGSTAIIAD 106

Query: 111 AAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHAS 170
           AAHSVSDVVLSG+AL S++AA  PKDKEHPYGHGKFETLGALGIS MLLAT  GIAWHA 
Sbjct: 107 AAHSVSDVVLSGVALVSYRAANVPKDKEHPYGHGKFETLGALGISAMLLATGSGIAWHAL 166

Query: 171 ELLLGLLSAAPEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWI 230
           +LL   LSAAPE++             H GHHHGIDM+HPILAL +T+ SISIKEGLYWI
Sbjct: 167 DLLSIALSAAPEVI-------------HSGHHHGIDMNHPILALTVTIASISIKEGLYWI 226

Query: 231 TKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAG 290
           TKRAGEKQGSGLM ANAWHHR+DAISS+VAL+GVGGSILGV FLDPLAGLVVS MI+ AG
Sbjct: 227 TKRAGEKQGSGLMMANAWHHRSDAISSLVALVGVGGSILGVNFLDPLAGLVVSTMIVNAG 286

Query: 291 LGSGHQSILELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPF 350
           L +GHQSILELVDAAIPA Q+EP+R+TILQVEGV GCHRLRGRRAGSSLYLDVHI VDPF
Sbjct: 287 LKTGHQSILELVDAAIPAQQLEPIRQTILQVEGVKGCHRLRGRRAGSSLYLDVHIVVDPF 346

Query: 351 LSVSAAHCIGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL 410
            SVS AH +GE  RR+I+ +HPEVSEVFIHIDP+   F  S  D       ++ + ++  
Sbjct: 347 SSVSVAHEVGEYVRRQINLNHPEVSEVFIHIDPAFLQFSCSTKDHD-----SITKESNIC 406

Query: 411 LNEKKIESMISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVA 470
              K +E+ +S I  SQ  EK+ ++RITPHLL  KILL+I V+MP  + +++ M  A  A
Sbjct: 407 QEIKHVEATVSDIFSSQLSEKLTIKRITPHLLHSKILLQIVVAMPSTMSIQDVMIAAEHA 466

Query: 471 EMQILKAASNIVHVSIQLRL 491
           E +ILKAA N+  VSIQL L
Sbjct: 467 EKEILKAAPNVARVSIQLSL 468

BLAST of MC09g0634 vs. ExPASy Swiss-Prot
Match: Q10LJ2 (Metal tolerance protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP2 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.3e-128
Identity = 232/330 (70.30%), Postives = 278/330 (84.24%), Query Frame = 0

Query: 55  ISRRWHLGHAHRHDDHHR---FGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADA 114
           +  R H+GH+H H  HH     GE  E IF+LGL AD+ L  GKAVTGYLSGSTAI ADA
Sbjct: 53  VPSRGHVGHSHHH--HHGEEVGGEASERIFRLGLAADVVLTVGKAVTGYLSGSTAIAADA 112

Query: 115 AHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASE 174
           AHS+SD+VLSG+AL S+KAAKAP+DKEHPYGHGKFE+LGALGIS MLL TAGGIAWHA +
Sbjct: 113 AHSLSDIVLSGVALLSYKAAKAPRDKEHPYGHGKFESLGALGISSMLLVTAGGIAWHAFD 172

Query: 175 LLLGLLSAAPEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWIT 234
           +L G++S+AP+I+   + H   H+H   GHHHGID++HPILAL++T  +IS+KEGLYWIT
Sbjct: 173 VLQGVMSSAPDIIGN-VSHAH-HSHGSSGHHHGIDLEHPILALSVTAFAISVKEGLYWIT 232

Query: 235 KRAGEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL 294
           KRAGEK+GSGLMKANAWHHR+DAISSVVAL+GVGGSILGV +LDPLAGLVVSGMILKAG+
Sbjct: 233 KRAGEKEGSGLMKANAWHHRSDAISSVVALLGVGGSILGVPYLDPLAGLVVSGMILKAGV 292

Query: 295 GSGHQSILELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFL 354
            +G++S+LELVDAA+    ++P++ TILQV+GV GCHRLRGR+AG+SLYLDVHIEV PFL
Sbjct: 293 HTGYESVLELVDAAVDPSLLQPIKETILQVDGVKGCHRLRGRKAGTSLYLDVHIEVYPFL 352

Query: 355 SVSAAHCIGENARRKIHTSHPEVSEVFIHI 382
           SVSAAH IGE  R +I  SH +V+EVFIHI
Sbjct: 353 SVSAAHDIGETVRHQIQKSHNQVAEVFIHI 378

BLAST of MC09g0634 vs. ExPASy Swiss-Prot
Match: Q2W8L4 (Magnetosome protein MamB OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=mamB PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.3e-28
Identity = 80/298 (26.85%), Postives = 140/298 (46.98%), Query Frame = 0

Query: 87  ADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKF 146
           ADI +   K V G +SGS A++AD+ HS +DVV SG+   S K +  P D+ +P+G+G  
Sbjct: 20  ADICMTLFKGVLGLMSGSVALVADSLHSGADVVASGVTQLSLKISNKPADERYPFGYGNI 79

Query: 147 ETLGALGISCMLLATAGGIAWHASELLLGLLSAAPEIVNQPLGHDDLHNHSHGGHHHGID 206
           + + +  +  +LL  A  + + +   L+     AP                         
Sbjct: 80  QYISSSIVGSLLLIGASFLMYGSVMKLISGTYEAPS------------------------ 139

Query: 207 MDHPILALNMTLVSISIKEGLYWITKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGG 266
               I A     V++ + E +Y      G +  S  + ANAW +R+DAISS   ++GV  
Sbjct: 140 ----IFAAVGASVTVIVNELMYRYQICVGNENNSPAIIANAWDNRSDAISSAAVMVGVIA 199

Query: 267 SILGVKFLDPLAGLVVSGMILKAGLGSGHQSILELVDAAIPADQIEPVRRTILQVEGVMG 326
           S++G    D +A + VS ++ + GL     SI  L+D+++  + ++   +  +    V  
Sbjct: 200 SVIGFPIADTIAAIGVSALVGRIGLELIGTSIHGLMDSSVDTELLQTAWQVAMDTPMVHS 259

Query: 327 CHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGENARRKIHTSHPEVSEVFIHIDPS 385
            + LRGR  G  +  D+ + VDP L +  +  + E  RR+I    P   ++ + + P+
Sbjct: 260 IYFLRGRHVGEDVQFDIRLRVDPNLRIKDSSMVAEAVRRRIQEEIPHARDIRLFVSPA 289

BLAST of MC09g0634 vs. ExPASy Swiss-Prot
Match: Q57891 (Uncharacterized transporter MJ0449 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0449 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.6e-28
Identity = 87/307 (28.34%), Postives = 148/307 (48.21%), Query Frame = 0

Query: 76  EGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAAKAPK 135
           E E    L +  +I L   K + GY+  S ++I+D  HS+SDV+ S I +   K A  P 
Sbjct: 3   EVEKPLILSIVGNILLGLIKIIIGYVYSSISLISDGIHSLSDVITSIIGIIGVKIASKPP 62

Query: 136 DKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAAPEIVNQPLGHDDLHN 195
           D+ HPYGH +FE L +  I   L  TA  I   A E ++                     
Sbjct: 63  DESHPYGHSRFECLFSFFIGLALFFTAYEIGKFAVERII--------------------- 122

Query: 196 HSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGSGLMKANAWHHRADAI 255
                  +G  ++   + + + ++SI +KE +   +   G+K  S ++ A+A+HHR+DA+
Sbjct: 123 -------YGEVIEVNAIMVGVAILSIVVKELMTRYSLFVGKKLNSQVLIADAYHHRSDAL 182

Query: 256 SSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILELVDAAIPADQIEPVR 315
           SSVV L+G+     G+ + D +AG++V+ MI K        +I  L   A P    E + 
Sbjct: 183 SSVVVLVGLLLQKFGIYYGDAIAGIIVALMIAKVAFDICLTNIDYLTGRAPPKKFFELIE 242

Query: 316 RTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIGENARRKIHTSHPEVS 375
           +  L V+ V+G H ++    G  +++++H+EV   +S    H I    + ++  S   V 
Sbjct: 243 KEALNVDKVIGVHDIKAHYVGPRIHVELHVEVPSNISAKEMHDIEVAVKNRLE-SLENVE 280

Query: 376 EVFIHID 383
             ++H+D
Sbjct: 303 RAYVHVD 280

BLAST of MC09g0634 vs. ExPASy Swiss-Prot
Match: Q08970 (Mitochondrial metal transporter 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MMT2 PE=3 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-27
Identity = 87/273 (31.87%), Postives = 143/273 (52.38%), Query Frame = 0

Query: 63  HAHRHDD-------HHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSV 122
           H H HD+              G  I  +GL +++ +A GK V G    S A++AD+ H++
Sbjct: 108 HTHMHDNPLLSLNVQQIKKNPGVRITWIGLASNVGMAVGKFVGGITFHSQALLADSVHAL 167

Query: 123 SDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLG 182
           SD+V   + L+S + A      E+PYG+GK ET+G+L +S +L      I W +   ++G
Sbjct: 168 SDLVSDFLTLFSVQYASRKPTSEYPYGYGKVETVGSLAVSTILAMAGISIGWSSLCAIVG 227

Query: 183 LLSAAPEIVNQPLGH--DDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKR 242
                P  + + +     + H+HS        +++    A+ +   SI +KE ++  TK+
Sbjct: 228 --PVIPHAILESMAGLIGETHSHSQSLTQQATNVN----AVWIAAGSILVKEWVFQATKK 287

Query: 243 AGEKQGSGLMKANAWHHRADAISSVVALIGVGGS-ILGVKFLDPLAGLVVSGMILKAGLG 302
              +  S ++ ANAWHHR D+++S+VAL+ +  S    ++ LD L GLVVSG+I+K G  
Sbjct: 288 VAIQTNSNVLMANAWHHRVDSLTSLVALVAITSSYFFNIQSLDNLGGLVVSGLIIKTGGQ 347

Query: 303 SGHQSILELVDAAIPADQIEPVRRTILQVEGVM 326
               S+ ELVD +IP     P     L++E V+
Sbjct: 348 GILSSLKELVDQSIP-----PTDPRYLEIESVI 369

BLAST of MC09g0634 vs. NCBI nr
Match: XP_022151544.1 (metal tolerance protein C1 [Momordica charantia])

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH
Sbjct: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI 420
           ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI
Sbjct: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI 420

Query: 421 SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN 480
           SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN
Sbjct: 421 SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN 480

Query: 481 IVHVSIQLRLGHRIPQLSHQAKF 503
           IVHVSIQLRLGHRIPQLSHQAKF
Sbjct: 481 IVHVSIQLRLGHRIPQLSHQAKF 503

BLAST of MC09g0634 vs. NCBI nr
Match: XP_038876738.1 (metal tolerance protein 2 [Benincasa hispida])

HSP 1 Score: 855 bits (2210), Expect = 1.58e-310
Identity = 430/501 (85.83%), Postives = 466/501 (93.01%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNP+LQSFYT+LSS TH+EFH+IP IQSLNL PQ +LLGIYDDP ++I RRWH
Sbjct: 6   MGYRFHRLNPVLQSFYTKLSSPTHKEFHAIPSIQSLNLHPQTTLLGIYDDPTSKICRRWH 65

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LAAGKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 66  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAAGKAVTGYLSGSTAIIADAAHSVSDVVL 125

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA +APKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 126 SGIALWSFKAGQAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 185

Query: 181 PEIVNQPLGHDDLHNHSH--GGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQ 240
           PEIVNQP GH+ LHNHSH  GGHHHGIDMDHPILALNMT++SI IKEGLYW+TKRAGEKQ
Sbjct: 186 PEIVNQPFGHESLHNHSHNHGGHHHGIDMDHPILALNMTIISICIKEGLYWMTKRAGEKQ 245

Query: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSI 300
           GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSI
Sbjct: 246 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSI 305

Query: 301 LELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 360
           LELVDAAIPADQI PV++TIL+VEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC
Sbjct: 306 LELVDAAIPADQINPVKQTILEVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 365

Query: 361 IGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIES 420
           IGEN R+KIHTSHPEVSEVFIHIDPSISHF   LSD Q GS GT NQ+TD  L EK IE+
Sbjct: 366 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPKLSDQQAGSTGTSNQNTDFPLIEKNIEA 425

Query: 421 MISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAA 480
           ++S II S+FPE M+VERITPHLLQGKILL+IEVSMPP+LL+RNAME+A+ AEM+ILKAA
Sbjct: 426 IVSEIILSKFPENMLVERITPHLLQGKILLQIEVSMPPNLLIRNAMEIAKQAEMEILKAA 485

Query: 481 SNIVHVSIQLRLGHRIPQLSH 499
           SNIVHVSIQLRLGH+IPQL+H
Sbjct: 486 SNIVHVSIQLRLGHQIPQLTH 506

BLAST of MC09g0634 vs. NCBI nr
Match: KAG6575848.1 (Metal tolerance protein C1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 846 bits (2185), Expect = 8.08e-307
Identity = 426/500 (85.20%), Postives = 462/500 (92.40%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNPILQSFY+RLSSATH+EFH+IP  QS +L PQ +LLGIY DPK++I RRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSSATHKEFHAIPSTQSFDLHPQATLLGIYGDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH+ LHNHSHGGHHHGIDMDHP+LALNMT++SISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPLLALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TILQVEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL-LNEKKIESM 420
           EN R++IHTSHPE SEVFIHIDPSISH    LSD Q GS GT NQ+ D + L EK IE++
Sbjct: 361 ENVRQRIHTSHPEASEVFIHIDPSISHVPLKLSDQQGGSAGTSNQNIDDIALEEKNIEAI 420

Query: 421 ISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAAS 480
           +S+II S+F E MVVERITPHLLQGKILL+IEVSMPPDLL+RNAMEVA+ AE+++LKAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLKAAS 480

Query: 481 NIVHVSIQLRLGHRIPQLSH 499
           NIVHVSI LRLGH+IP L+H
Sbjct: 481 NIVHVSILLRLGHQIPHLTH 500

BLAST of MC09g0634 vs. NCBI nr
Match: XP_022954108.1 (metal tolerance protein 2 [Cucurbita moschata])

HSP 1 Score: 841 bits (2173), Expect = 5.43e-305
Identity = 425/500 (85.00%), Postives = 460/500 (92.00%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNPILQSFY+RLS ATH+EFH+IP  QS +L PQ +LLGIY DPK++I RRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSLATHKEFHAIPSTQSFDLHPQATLLGIYGDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH+ LHNHSHGGHHHGIDMDHPILALNMT++SISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTILSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TILQVEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL-LNEKKIESM 420
           EN R++IHTSHPE SEVFIHIDPSISH    LSD Q GS GT NQ+ D + L EK IE++
Sbjct: 361 ENVRQRIHTSHPEASEVFIHIDPSISHVPLKLSDQQGGSAGTSNQNIDDIALEEKNIEAI 420

Query: 421 ISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAAS 480
           +S+II S+F E MVVERITPHLLQGKILL+IEVSMPPDLL+RNAMEVA+ AE+++L AAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLMAAS 480

Query: 481 NIVHVSIQLRLGHRIPQLSH 499
           NIVHVSI LRLGH+IP L+H
Sbjct: 481 NIVHVSILLRLGHQIPHLTH 500

BLAST of MC09g0634 vs. NCBI nr
Match: XP_023548946.1 (metal tolerance protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 840 bits (2171), Expect = 1.09e-304
Identity = 423/500 (84.60%), Postives = 461/500 (92.20%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNPILQSFY+RLSSA+H+EFH+IP  QS +L PQ +LLGIY DPK++I RRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSSASHKEFHAIPSTQSFDLHPQTTLLGIYGDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH+ LHNHSHGGHHHGIDM+HP+LALNMT++SISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMNHPLLALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TILQVEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQST-DTLLNEKKIESM 420
           EN R++IHTSHPE SEVFIHIDPS+SH    LSD Q GS GT NQ+  D  L EK IE++
Sbjct: 361 ENVRQQIHTSHPEASEVFIHIDPSMSHVPLKLSDQQGGSAGTSNQNIGDIALEEKNIEAI 420

Query: 421 ISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAAS 480
           +S+II S+F E MVVERITPHLLQGKILL+IEVSMPPDLL+RNAMEVA+ AE+++LKAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLKAAS 480

Query: 481 NIVHVSIQLRLGHRIPQLSH 499
           NIVHVSI LRLGH+IP L+H
Sbjct: 481 NIVHVSILLRLGHQIPHLTH 500

BLAST of MC09g0634 vs. ExPASy TrEMBL
Match: A0A6J1DDD3 (metal tolerance protein C1 OS=Momordica charantia OX=3673 GN=LOC111019455 PE=4 SV=1)

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH
Sbjct: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI 420
           ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI
Sbjct: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIESMI 420

Query: 421 SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN 480
           SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN
Sbjct: 421 SRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAASN 480

Query: 481 IVHVSIQLRLGHRIPQLSHQAKF 503
           IVHVSIQLRLGHRIPQLSHQAKF
Sbjct: 481 IVHVSIQLRLGHRIPQLSHQAKF 503

BLAST of MC09g0634 vs. ExPASy TrEMBL
Match: A0A6J1GQ72 (metal tolerance protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111456474 PE=4 SV=1)

HSP 1 Score: 841 bits (2173), Expect = 2.63e-305
Identity = 425/500 (85.00%), Postives = 460/500 (92.00%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNPILQSFY+RLS ATH+EFH+IP  QS +L PQ +LLGIY DPK++I RRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRLSLATHKEFHAIPSTQSFDLHPQATLLGIYGDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH+ LHNHSHGGHHHGIDMDHPILALNMT++SISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTILSISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TILQVEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL-LNEKKIESM 420
           EN R++IHTSHPE SEVFIHIDPSISH    LSD Q GS GT NQ+ D + L EK IE++
Sbjct: 361 ENVRQRIHTSHPEASEVFIHIDPSISHVPLKLSDQQGGSAGTSNQNIDDIALEEKNIEAI 420

Query: 421 ISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAAS 480
           +S+II S+F E MVVERITPHLLQGKILL+IEVSMPPDLL+RNAMEVA+ AE+++L AAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMEVAKQAEIEVLMAAS 480

Query: 481 NIVHVSIQLRLGHRIPQLSH 499
           NIVHVSI LRLGH+IP L+H
Sbjct: 481 NIVHVSILLRLGHQIPHLTH 500

BLAST of MC09g0634 vs. ExPASy TrEMBL
Match: A0A6J1JQY4 (metal tolerance protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111488105 PE=4 SV=1)

HSP 1 Score: 836 bits (2160), Expect = 2.51e-303
Identity = 421/500 (84.20%), Postives = 459/500 (91.80%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNPILQSF++RLSSA+H+EFH+IP  QS +L PQ +LLGIY DPK++I RRWH
Sbjct: 1   MGYRFHRLNPILQSFHSRLSSASHKEFHAIPSTQSFDLHPQTTLLGIYGDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKA TGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAFTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGS 240
           PEIVNQPLGH+ LHNHSHGGHHHGIDMDHPILALNMT++SISIKEGLYWITKRAGEKQGS
Sbjct: 181 PEIVNQPLGHESLHNHSHGGHHHGIDMDHPILALNMTIISISIKEGLYWITKRAGEKQGS 240

Query: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSILE 300
           GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +G+QSILE
Sbjct: 241 GLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLETGYQSILE 300

Query: 301 LVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360
           LVDAAIPADQI+PVR+TILQVEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG
Sbjct: 301 LVDAAIPADQIDPVRKTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHCIG 360

Query: 361 ENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL-LNEKKIESM 420
           EN R++IHTSHPE SEVFIHIDPSISH     SD Q G+ GT NQ+ D + L EK IE++
Sbjct: 361 ENVRQQIHTSHPEASEVFIHIDPSISHVPLKFSDQQGGTAGTSNQNIDDIALEEKNIEAI 420

Query: 421 ISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAAS 480
           +S+II S+F E MVVERITPHLLQ KILL+IEVSMPPDLL+RNAMEVA+ AE+++ KAAS
Sbjct: 421 VSKIILSKFHESMVVERITPHLLQSKILLQIEVSMPPDLLIRNAMEVAKQAEIEVSKAAS 480

Query: 481 NIVHVSIQLRLGHRIPQLSH 499
           NIVHVSI LRLGH+IP L+H
Sbjct: 481 NIVHVSILLRLGHQIPHLTH 500

BLAST of MC09g0634 vs. ExPASy TrEMBL
Match: A0A1S3BRB4 (metal tolerance protein C1 OS=Cucumis melo OX=3656 GN=LOC103492849 PE=4 SV=1)

HSP 1 Score: 832 bits (2150), Expect = 8.68e-302
Identity = 424/501 (84.63%), Postives = 455/501 (90.82%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNP+LQSFY+R+S  T  EFHSIP  QSLNL PQ +LLGIYDDPK++I RRWH
Sbjct: 1   MGYRFHRLNPMLQSFYSRISPPTRNEFHSIPSFQSLNLHPQITLLGIYDDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SG+ALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGVALWSFKAGKAPKDKEHPYGHGKFETLGALGISTMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSH--GGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQ 240
           PEIVNQP G + LHNHSH  G HHHGIDMDHPILALNMT++SI IKEGLYWITKRAGEKQ
Sbjct: 181 PEIVNQPFGLESLHNHSHSHGEHHHGIDMDHPILALNMTIISICIKEGLYWITKRAGEKQ 240

Query: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSI 300
           GSGLMKANAWHH ADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +GHQSI
Sbjct: 241 GSGLMKANAWHHCADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLQTGHQSI 300

Query: 301 LELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 360
           LELVDAAIPADQI+P ++TILQ+EGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAH 
Sbjct: 301 LELVDAAIPADQIDPFKQTILQIEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHS 360

Query: 361 IGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIES 420
           IGEN R+KIHTSHPEVSEVFIHIDPSISHF   LS+ Q  S GT NQST+ LL EK IE+
Sbjct: 361 IGENVRQKIHTSHPEVSEVFIHIDPSISHFPPKLSNKQASSTGTSNQSTNFLLTEKNIEA 420

Query: 421 MISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAA 480
            +S II S+FPE MVVERITPHLLQGKILL+IEVSMPPDLL+RNAM+VA+ AE +ILKA 
Sbjct: 421 TVSDIILSKFPENMVVERITPHLLQGKILLQIEVSMPPDLLIRNAMDVAKRAENEILKAD 480

Query: 481 SNIVHVSIQLRLGHRIPQLSH 499
           SNIVHVSIQLRLGH+IPQLSH
Sbjct: 481 SNIVHVSIQLRLGHQIPQLSH 501

BLAST of MC09g0634 vs. ExPASy TrEMBL
Match: A0A0A0K5Y9 (Metal tolerance protein 6 OS=Cucumis sativus OX=3659 GN=MTP6 PE=2 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 1.38e-300
Identity = 423/501 (84.43%), Postives = 455/501 (90.82%), Query Frame = 0

Query: 1   MRYRFYRLNPILQSFYTRLSSATHQEFHSIPPIQSLNLQPQNSLLGIYDDPKNRISRRWH 60
           M YRF+RLNPILQSFY+R+S  TH+EFHSIP  QS +L PQ + LGIYDDPK++I RRWH
Sbjct: 1   MGYRFHRLNPILQSFYSRISPPTHKEFHSIPSFQSPSLHPQFTFLGIYDDPKSKICRRWH 60

Query: 61  LGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIADAAHSVSDVVL 120
           LGH+HRHDD HRFG+EGE+IFKLGLGADI LA GKAVTGYLSGSTAIIADAAHSVSDVVL
Sbjct: 61  LGHSHRHDDDHRFGQEGENIFKLGLGADIGLAVGKAVTGYLSGSTAIIADAAHSVSDVVL 120

Query: 121 SGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHASELLLGLLSAA 180
           SGIALWSFKA KAPKDKEHPYGHGKFETLGALGIS MLLATAGGIAWHASELLLGLLSAA
Sbjct: 121 SGIALWSFKAGKAPKDKEHPYGHGKFETLGALGISSMLLATAGGIAWHASELLLGLLSAA 180

Query: 181 PEIVNQPLGHDDLHNHSH--GGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQ 240
           PEIVNQP GH+ LHNHSH  G HHHGIDMDHPILALNMT++SI IKEGLYWITKRAGEK+
Sbjct: 181 PEIVNQPFGHESLHNHSHSHGEHHHGIDMDHPILALNMTIISICIKEGLYWITKRAGEKR 240

Query: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLGSGHQSI 300
           GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGL +GHQSI
Sbjct: 241 GSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAGLQTGHQSI 300

Query: 301 LELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHC 360
           LELVDAAIPADQI+P ++TILQVEGV GCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAH 
Sbjct: 301 LELVDAAIPADQIDPFKQTILQVEGVKGCHRLRGRRAGSSLYLDVHIEVDPFLSVSAAHS 360

Query: 361 IGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTLLNEKKIES 420
           IGEN R +IHTSHPEVSEVFIHIDPSISHF   LS+ Q  S GT NQSTD  L E  IE+
Sbjct: 361 IGENVRHEIHTSHPEVSEVFIHIDPSISHFPPKLSNQQAASAGTSNQSTDFPLTEN-IEA 420

Query: 421 MISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVAEMQILKAA 480
            +S I+QS+FPE M+VERITPHLLQGKILL+IEVSMPPDLL+RNAM+VA+ AEM+ILKA 
Sbjct: 421 TVSDIVQSKFPENMMVERITPHLLQGKILLQIEVSMPPDLLIRNAMDVAKRAEMEILKAD 480

Query: 481 SNIVHVSIQLRLGHRIPQLSH 499
           SNIVHVSIQLRLG +IPQLSH
Sbjct: 481 SNIVHVSIQLRLGQQIPQLSH 500

BLAST of MC09g0634 vs. TAIR 10
Match: AT2G47830.1 (Cation efflux family protein )

HSP 1 Score: 552.7 bits (1423), Expect = 2.9e-157
Identity = 291/440 (66.14%), Postives = 337/440 (76.59%), Query Frame = 0

Query: 51  PKNRISRRWHLGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIAD 110
           P    SRRWH GH   H  + + GEEGE IF+LGL ADI L+  KA+TGYL GSTAIIAD
Sbjct: 47  PSFDFSRRWHFGHPDHHQQYQKPGEEGEKIFRLGLTADIGLSVAKALTGYLCGSTAIIAD 106

Query: 111 AAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHAS 170
           AAHSVSDVVLSG+AL S++AA  PKDKEHPYGHGKFETLGALGIS MLLAT  GIAWHA 
Sbjct: 107 AAHSVSDVVLSGVALVSYRAANVPKDKEHPYGHGKFETLGALGISAMLLATGSGIAWHAL 166

Query: 171 ELLLGLLSAAPEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWI 230
           +LL   LSAAPE++             H GHHHGIDM+HPILAL +T+ SISIKEGLYWI
Sbjct: 167 DLLSIALSAAPEVI-------------HSGHHHGIDMNHPILALTVTIASISIKEGLYWI 226

Query: 231 TKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAG 290
           TKRAGEKQGSGLM ANAWHHR+DAISS+VAL+GVGGSILGV FLDPLAGLVVS MI+ AG
Sbjct: 227 TKRAGEKQGSGLMMANAWHHRSDAISSLVALVGVGGSILGVNFLDPLAGLVVSTMIVNAG 286

Query: 291 LGSGHQSILELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPF 350
           L +GHQSILELVDAAIPA Q+EP+R+TILQVEGV GCHRLRGRRAGSSLYLDVHI VDPF
Sbjct: 287 LKTGHQSILELVDAAIPAQQLEPIRQTILQVEGVKGCHRLRGRRAGSSLYLDVHIVVDPF 346

Query: 351 LSVSAAHCIGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL 410
            SVS AH +GE  RR+I+ +HPEVSEVFIHIDP+   F  S  D       ++ + ++  
Sbjct: 347 SSVSVAHEVGEYVRRQINLNHPEVSEVFIHIDPAFLQFSCSTKDHD-----SITKESNIC 406

Query: 411 LNEKKIESMISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVA 470
              K +E+ +S I  SQ  EK+ ++RITPHLL  KILL+I V+MP  + +++ M  A  A
Sbjct: 407 QEIKHVEATVSDIFSSQLSEKLTIKRITPHLLHSKILLQIVVAMPSTMSIQDVMIAAEHA 466

Query: 471 EMQILKAASNIVHVSIQLRL 491
           E +ILKAA N+  VSIQL L
Sbjct: 467 EKEILKAAPNVARVSIQLSL 468

BLAST of MC09g0634 vs. TAIR 10
Match: AT2G47830.2 (Cation efflux family protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.6e-155
Identity = 291/440 (66.14%), Postives = 335/440 (76.14%), Query Frame = 0

Query: 51  PKNRISRRWHLGHAHRHDDHHRFGEEGESIFKLGLGADIALAAGKAVTGYLSGSTAIIAD 110
           P    SRRWH GH   H  + + GEEGE IF+LGL ADI L+  KA+TGYL GSTAIIAD
Sbjct: 47  PSFDFSRRWHFGHPDHHQQYQKPGEEGEKIFRLGLTADIGLSVAKALTGYLCGSTAIIAD 106

Query: 111 AAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISCMLLATAGGIAWHAS 170
           AAHSVSDVVLSG+AL S++AA  PKDKEHPYGHGKFETLGALGIS MLLAT  GIAWHA 
Sbjct: 107 AAHSVSDVVLSGVALVSYRAANVPKDKEHPYGHGKFETLGALGISAMLLATGSGIAWHAL 166

Query: 171 ELLLGLLSAAPEIVNQPLGHDDLHNHSHGGHHHGIDMDHPILALNMTLVSISIKEGLYWI 230
           +LL   LSAAPE++             H GHHHGIDM+HPILAL +T+ SISIKEGLYWI
Sbjct: 167 DLLSIALSAAPEVI-------------HSGHHHGIDMNHPILALTVTIASISIKEGLYWI 226

Query: 231 TKRAGEKQGSGLMKANAWHHRADAISSVVALIGVGGSILGVKFLDPLAGLVVSGMILKAG 290
           TKRAGEKQGSGLM ANAWHHR+DAISS+VAL+GVGGSILGV FLDPLAGLVVS MI+ AG
Sbjct: 227 TKRAGEKQGSGLMMANAWHHRSDAISSLVALVGVGGSILGVNFLDPLAGLVVSTMIVNAG 286

Query: 291 LGSGHQSILELVDAAIPADQIEPVRRTILQVEGVMGCHRLRGRRAGSSLYLDVHIEVDPF 350
           L +GHQSILELVDAAIPA Q+EP+R+TILQVEGV GCHRLRGRRAGSSLYLDVHI VDPF
Sbjct: 287 LKTGHQSILELVDAAIPAQQLEPIRQTILQVEGVKGCHRLRGRRAGSSLYLDVHIVVDPF 346

Query: 351 LSVSAAHCIGENARRKIHTSHPEVSEVFIHIDPSISHFQHSLSDWQEGSIGTLNQSTDTL 410
            SVS AH +GE  RR+I+ +HPEVSEVFIHIDP+   F  S  D       ++ + ++  
Sbjct: 347 SSVSVAHEVGEYVRRQINLNHPEVSEVFIHIDPAFLQFSCSTKDHD-----SITKESNIC 406

Query: 411 LNEKKIESMISRIIQSQFPEKMVVERITPHLLQGKILLEIEVSMPPDLLVRNAMEVARVA 470
              K +E+ +S I  SQ  EK+ ++RITPHLL  KILL+I V+MP  +   + M  A  A
Sbjct: 407 QEIKHVEATVSDIFSSQLSEKLTIKRITPHLLHSKILLQIVVAMPSTM---DVMIAAEHA 465

Query: 471 EMQILKAASNIVHVSIQLRL 491
           E +ILKAA N+  VSIQL L
Sbjct: 467 EKEILKAAPNVARVSIQLSL 465

BLAST of MC09g0634 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 66.2 bits (160), Expect = 8.4e-11
Identity = 81/280 (28.93%), Postives = 127/280 (45.36%), Query Frame = 0

Query: 95  KAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGI 154
           + V G  + S AI+ DAAH +SDV    I+L+S  A+    + +  YG  + E LGAL  
Sbjct: 127 EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVS 186

Query: 155 SCMLLATAGGIAWHA-----------SELLLGLLSAAPEIVNQP----LGHDDLHNHSH- 214
             M+   AG + + A              L+  +SA   +VN      LGHD  H H H 
Sbjct: 187 IQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHS 246

Query: 215 --GGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGSGLMKANAWHHRADAIS 274
              GH H  D  H I A      S   +  L  +     +KQ +  ++    H   D+I 
Sbjct: 247 HDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQ-KKQRNVNIQGAYLHVLGDSIQ 306

Query: 275 SVVALIGVGGSILGV----KFLDPLAGLVVSGMILKAGLGSGHQSILELVDAAIPADQIE 334
           SV  +I  GG+I+      K LD +  LV S ++L   +G   ++ILE++  + P  +I+
Sbjct: 307 SVGVMI--GGAIIWYKPEWKILDLICTLVFSVIVLGTTIGM-LRNILEVLMESTPR-EID 366

Query: 335 P--VRRTILQVEGVMGCHRLR-GRRAGSSLYLDVHIEVDP 350
           P  + + + ++E V+  H L         L L  H+++ P
Sbjct: 367 PTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRP 401

BLAST of MC09g0634 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 66.2 bits (160), Expect = 8.4e-11
Identity = 81/280 (28.93%), Postives = 127/280 (45.36%), Query Frame = 0

Query: 95  KAVTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGI 154
           + V G  + S AI+ DAAH +SDV    I+L+S  A+    + +  YG  + E LGAL  
Sbjct: 88  EVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVS 147

Query: 155 SCMLLATAGGIAWHA-----------SELLLGLLSAAPEIVNQP----LGHDDLHNHSH- 214
             M+   AG + + A              L+  +SA   +VN      LGHD  H H H 
Sbjct: 148 IQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHS 207

Query: 215 --GGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGSGLMKANAWHHRADAIS 274
              GH H  D  H I A      S   +  L  +     +KQ +  ++    H   D+I 
Sbjct: 208 HDNGHGHSHDHGHGIAATEHHHDSGHDESQLSDVLIEQ-KKQRNVNIQGAYLHVLGDSIQ 267

Query: 275 SVVALIGVGGSILGV----KFLDPLAGLVVSGMILKAGLGSGHQSILELVDAAIPADQIE 334
           SV  +I  GG+I+      K LD +  LV S ++L   +G   ++ILE++  + P  +I+
Sbjct: 268 SVGVMI--GGAIIWYKPEWKILDLICTLVFSVIVLGTTIGM-LRNILEVLMESTPR-EID 327

Query: 335 P--VRRTILQVEGVMGCHRLR-GRRAGSSLYLDVHIEVDP 350
           P  + + + ++E V+  H L         L L  H+++ P
Sbjct: 328 PTMLEKGVCEIEEVVAVHELHIWAITVGKLLLACHVKIRP 362

BLAST of MC09g0634 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 57.0 bits (136), Expect = 5.1e-08
Identity = 70/255 (27.45%), Postives = 113/255 (44.31%), Query Frame = 0

Query: 97  VTGYLSGSTAIIADAAHSVSDVVLSGIALWSFKAAKAPKDKEHPYGHGKFETLGALGISC 156
           V G+ + S A++ DAAH +SDV    ++L + K +    +  + +G  + E L A  +S 
Sbjct: 74  VGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAF-LSV 133

Query: 157 MLLATAGGIAWHASELLLGLLSAAPEIVNQP------------------LGHDDLHNHSH 216
            L+    G+  H  E +  LLS + E+  +                   LGH+  H+H H
Sbjct: 134 QLIWLVSGVIIH--EAIQRLLSRSREVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHH-H 193

Query: 217 GGHHHGIDMDHPILALNMTLVSISIKEGLYWITKRAGEKQGSGLMKANA----WHHRADA 276
             HHH  +  H     +  +V+   +E +       GEK  S  M  N      H  AD 
Sbjct: 194 DHHHHHHNHKHQHQHHHKEVVAEEEEEEM---NPLKGEKSSSKEMNINIQGAYLHAMADM 253

Query: 277 ISSVVALIGVGGSILGVK----FLDPLAGLVVSGMILKAGLGSGHQSILELVDAAIPAD- 325
           I S+  +I  GG I+ VK     +D +  LV S   L A L    ++I  ++   +P D 
Sbjct: 254 IQSLGVMI--GGGIIWVKPKWVLVDLICTLVFSAFALAATLPI-LKNIFGILMERVPRDM 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7254.1e-15666.14Metal tolerance protein C1 OS=Arabidopsis thaliana OX=3702 GN=MTPC1 PE=2 SV=1[more]
Q10LJ21.3e-12870.30Metal tolerance protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP2 PE=2 ... [more]
Q2W8L43.3e-2826.85Magnetosome protein MamB OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700... [more]
Q578915.6e-2828.34Uncharacterized transporter MJ0449 OS=Methanocaldococcus jannaschii (strain ATCC... [more]
Q089701.3e-2731.87Mitochondrial metal transporter 2 OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
Match NameE-valueIdentityDescription
XP_022151544.10.0100.00metal tolerance protein C1 [Momordica charantia][more]
XP_038876738.11.58e-31085.83metal tolerance protein 2 [Benincasa hispida][more]
KAG6575848.18.08e-30785.20Metal tolerance protein C1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022954108.15.43e-30585.00metal tolerance protein 2 [Cucurbita moschata][more]
XP_023548946.11.09e-30484.60metal tolerance protein 2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DDD30.0100.00metal tolerance protein C1 OS=Momordica charantia OX=3673 GN=LOC111019455 PE=4 S... [more]
A0A6J1GQ722.63e-30585.00metal tolerance protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111456474 PE=4 SV=... [more]
A0A6J1JQY42.51e-30384.20metal tolerance protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111488105 PE=4 SV=1[more]
A0A1S3BRB48.68e-30284.63metal tolerance protein C1 OS=Cucumis melo OX=3656 GN=LOC103492849 PE=4 SV=1[more]
A0A0A0K5Y91.38e-30084.43Metal tolerance protein 6 OS=Cucumis sativus OX=3659 GN=MTP6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47830.12.9e-15766.14Cation efflux family protein [more]
AT2G47830.21.6e-15566.14Cation efflux family protein [more]
AT3G58810.18.4e-1128.93metal tolerance protein A2 [more]
AT3G58810.28.4e-1128.93metal tolerance protein A2 [more]
AT2G29410.15.1e-0827.45metal tolerance protein B1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027470Cation efflux protein, cytoplasmic domainPFAMPF16916ZT_dimercoord: 307..383
e-value: 2.7E-21
score: 75.5
IPR036837Cation efflux protein, cytoplasmic domain superfamilyGENE3D3.30.70.1350Cation efflux protein, cytoplasmic domaincoord: 310..397
e-value: 2.4E-23
score: 84.0
coord: 412..501
e-value: 3.7E-6
score: 28.7
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 306..383
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 80..301
e-value: 1.4E-51
score: 176.9
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 80..303
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 83..302
e-value: 1.5E-44
score: 152.2
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 78..384
e-value: 1.4E-82
score: 275.1
NoneNo IPR availablePANTHERPTHR43840:SF15MITOCHONDRIAL METAL TRANSPORTER 1-RELATEDcoord: 63..384
NoneNo IPR availablePANTHERPTHR43840MITOCHONDRIAL METAL TRANSPORTER 1-RELATEDcoord: 63..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0634.1MC09g0634.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008324 cation transmembrane transporter activity