MC09g0628 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0628
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionautophagy-related protein 18a
LocationMC09: 5714387 .. 5717795 (-)
RNA-Seq ExpressionMC09g0628
SyntenyMC09g0628
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGGAGACTAAATAAACACAATTTTAAAATTCGACGTGATTTTACAAAATTGCCCTTGTAGTTAATAGGAAGCCGACAGATTGACGATTAACTCAGCATCGCTTGGGGAGAAAGCTAATTACGTCGCGTCCAAATACGTGTCCCTCACTCGCCGAATAATGGCGGAATCAACAGGCGTCACGAAATGACGAAACTACCCCCATCTTCCTTCCCGAAGCATTTCTCAATCCCGAACCCAAATCCGTAACTCCTCTCTCCCCCATCCCCCATTACTCGAAGCTCCAATTTTGTTCGTGTTCTTCGCTTCGTTTTCATCTGTAAATTGAAATCCCCAATTCCTCGAATTTCCCCCTCCCATGGAGACCCTCTCCGCCTTCCCCTCCCCTCCATGGCCCAACCCAAACCCAAGCCCTAATCCCAACCCCCCGCTTCCGGAGAACTCCGACCGCTTCGATTCCGATTCCGTTTCATCGGCCGAGCCCCCGGAGCCCAAGGTTCAGACCGTTCTGCCGGCGCCGGGCGACTCGCTCGCGTCGCTGCCCTCGCTGCTCCATCTCTCCTTCAATCAAGATCATGGCTGCTTTGCTGCGGGCACTGACCGGGGCTTTCGGATCTACAACTGCGATCCGTTCCGCGAGATTTTCAGGAGGGATTTCGATCGCGGGGGCGGCGTTGGCGTCGTGGAGATGCTTTTCCGGTGCAATATCTTGGCCCTTGTTGGCGGTGGGCCTGACCCTCAATACCCGCCTAATAAGGTCATGATTTGGGACGACCATCAGAGCCGATGCATTGGGGAGCTGTCGTTTCGCTCTGTTGTTCGTGGTGTGCGGCTGCAGAGGGACCGGATTATTGTGATTTTGGAGCAGAAGGTTTTCGTTTACAATTTTGCGGACTTGAAGCTGCTGCATCAGATCGAAACTATTGCGAACCCTAAGGGGCTTTGTGCGGTTTCCCAGTTGAGTACGTCGCTTGTGCTCGTTTGCCCCGGACTGCAGAAGGGGCAGGTTAGGGTTGAGCATTACGCCTCTCGACGGACCAAGTTCATAATGGCCCACGACTCGAGGATTGCTTGCTTTGCGCTTACGACCAATGGGCAGTTGCTTGCCACGGCGAGCAGCAAGGGAACTCTGGTTCGGATATTCAACACATTTGATGGAAATCTGCTTCAAGAGGTGTGTTTAATCGTCTTGAATTGAAGTTAATTCTTCTGGGTTTGTTATGACAATGATGTTTCATAATCTGCAATCTGATTCTTGAGTTTGTAACAATCAATTGTTCGTGGTCTTTTATGAAGTACTTAACATGGCCATGAATTTTCTTTGTGTTATCTGGGGTATGATGCATAGGTAAGGAGAGGCGCAGATAGAGCGGAGATCTACAGTTTGGCCTTTTCATCGACTGCCCAATGGCTGGCAGTCTCCAGTGACAAGGGAACCGTCCATGTATTCAGCCTTAAAGTCAATTCTGGATCTCTTGGAAATGATATGTCTCAGAATGATTCTTCTCTTTCTGTTGCACCAACTGGCTCCTCATTTTCCTTCATCAAAGGTAATTGAACTTGAACCTTTCCTATGATAATAAAGAAAAATGAGAATTGAAAAACATAACGCCATTTCACTGGCACCCTCTAGAAAAGAATGCTTGTACTTTGTATTTTTCATATCTCTTTCAATAATTCCTGGCAGAATAAGACCAGAAATTCTACATTAAAGGAAAGAGTTTGGAGAACCTGCTGATTGCCATTATCCCTGTTATTTTGCAGCTTAGCTTAGTTATGGAAACTAATGTCTGATATCTTTTTTTGCCTCTGGATCTAATTTTGTCTTGCATCTGCAGGTGTGTTGCCAAAGTATTTCAAGTCAGAGTGGTCTGTTGCTCAGTTTCGTTTGCATGAAGGCTCCCAGTATGTTGTTGCTTTTGGTCACCAGAAGAATACAGTGGTAATCCTTGGAATGGATGGAAGGTAAGTTGTCATCGGATGGGAATGGTGAAACTTAGTCGATAGCATCTCTTTAAATTGTTGATTATTTTTCATAATAACAATTAAGATGAGGATTGCTTGGAAAACCTAACTACCTGTTTATTCAAATTTTATGTTACCAGATAAATATCTAGCTAATTTTGAGATGTTTATTTGCATAGGCTGGAAATGGATAATTCTTTTGCCGTTTAACTCGAATCCATTTGTCCTTCCTACATTACCTTTTCACTAGCATATCTTTTTTTTTTCCCCTTTGATAATTGAGAGTTAGGAGTTTTGCTCCCTATATCTAGTGGCACGCGCACCTGCCCCAAGGTCTGGACCCGACACCCGAGAGTCATCAAGGGTTTTTGGTATTAAGCTCCTCACTGGAAGATTTGAACTTGAGACCCCTCTAAGTTAGTTTGACTTGGAGATCCCAAGCCTTTGCCAATCGGACTGGCCCCTGGGGCATCTTTTCATTAGCATTATCATAAATTGTGAAAGCTTCCATTAGATGTAAATCTCTCTCGAAGTCTTAGCATTATCTTAATCGATCATTCTGGTATCCTTGATGACTCCCCCTCAATTCTTCCCTGTTATGGCTCATTTTTTCCCTCTCAAATCTTCATCACTTGCTTTCTAGTTGTAAGGGTGTATGCTAGACTGCCTAAACTTGTTCGATGCATTTTATTTTACTCCCTCATGTGTCATTTTCTTATTGTATGCAGCTTCTATAGATGCCAATTTGATCCAGCGACAGGAGGAGAGATGACACAGCTCGAATATCACAACTTTCTAATGCCTGAGGAAGCCTTCTAGCCGAATATTCTCACTAATTTCGGTTATGTCACGTGTATGTTCTTTTCCTGTGTGCTTAAAGCATGTTGTAAATAGATCTATGTATGTGTACAGATTGGCCTATGTTCGTCGAAAAACAAGAAAAAGAAAAAAAAAAAAAAAGGAAAAGAATGCTACTATCGGTACTCATGTTCGTATATAATTGCTCTTTCTGTATTTGCCTTGTAAACACTACAGGGGCTATACTTCTAAAAGGTGAAGGAGAAGAAAGATGACTCGTAGAAACCACTTGAGATAAATTCATTGTGAATATAACAGCATTTGAATGAGTGTAATATCAGCTTCCTGAACTTTCCTTATGAAATTGAGTTGGCGTTGACTGCATATGATGCATGACTAAAGTTTACTATTTAAGTTTAGATTCTTTTAAGAAAAGTCTGAAGGGAAAGTTACCCAAAATGCTTTTTTACTTCGCCCTTTGTTTAAAATGCCCCTATCTCTCAAAGTTTCAATAGTGTCCTTGACTCGTGGCAAAATTTGGAATGACGTGACAATTAGTGCTGAAGTTTGCTTTGATTAAGTTTCTAAAGAGAAGAAATGTGGGAGAAAATATCATTTTTAAAACGAGTGTCGGGATAGACAAGCGAG

mRNA sequence

CGGGAGACTAAATAAACACAATTTTAAAATTCGACGTGATTTTACAAAATTGCCCTTGTAGTTAATAGGAAGCCGACAGATTGACGATTAACTCAGCATCGCTTGGGGAGAAAGCTAATTACGTCGCGTCCAAATACGTGTCCCTCACTCGCCGAATAATGGCGGAATCAACAGGCGTCACGAAATGACGAAACTACCCCCATCTTCCTTCCCGAAGCATTTCTCAATCCCGAACCCAAATCCGTAACTCCTCTCTCCCCCATCCCCCATTACTCGAAGCTCCAATTTTGTTCGTGTTCTTCGCTTCGTTTTCATCTGTAAATTGAAATCCCCAATTCCTCGAATTTCCCCCTCCCATGGAGACCCTCTCCGCCTTCCCCTCCCCTCCATGGCCCAACCCAAACCCAAGCCCTAATCCCAACCCCCCGCTTCCGGAGAACTCCGACCGCTTCGATTCCGATTCCGTTTCATCGGCCGAGCCCCCGGAGCCCAAGGTTCAGACCGTTCTGCCGGCGCCGGGCGACTCGCTCGCGTCGCTGCCCTCGCTGCTCCATCTCTCCTTCAATCAAGATCATGGCTGCTTTGCTGCGGGCACTGACCGGGGCTTTCGGATCTACAACTGCGATCCGTTCCGCGAGATTTTCAGGAGGGATTTCGATCGCGGGGGCGGCGTTGGCGTCGTGGAGATGCTTTTCCGGTGCAATATCTTGGCCCTTGTTGGCGGTGGGCCTGACCCTCAATACCCGCCTAATAAGGTCATGATTTGGGACGACCATCAGAGCCGATGCATTGGGGAGCTGTCGTTTCGCTCTGTTGTTCGTGGTGTGCGGCTGCAGAGGGACCGGATTATTGTGATTTTGGAGCAGAAGGTTTTCGTTTACAATTTTGCGGACTTGAAGCTGCTGCATCAGATCGAAACTATTGCGAACCCTAAGGGGCTTTGTGCGGTTTCCCAGTTGAGTACGTCGCTTGTGCTCGTTTGCCCCGGACTGCAGAAGGGGCAGGTTAGGGTTGAGCATTACGCCTCTCGACGGACCAAGTTCATAATGGCCCACGACTCGAGGATTGCTTGCTTTGCGCTTACGACCAATGGGCAGTTGCTTGCCACGGCGAGCAGCAAGGGAACTCTGGTTCGGATATTCAACACATTTGATGGAAATCTGCTTCAAGAGGTAAGGAGAGGCGCAGATAGAGCGGAGATCTACAGTTTGGCCTTTTCATCGACTGCCCAATGGCTGGCAGTCTCCAGTGACAAGGGAACCGTCCATGTATTCAGCCTTAAAGTCAATTCTGGATCTCTTGGAAATGATATGTCTCAGAATGATTCTTCTCTTTCTGTTGCACCAACTGGCTCCTCATTTTCCTTCATCAAAGGTGTGTTGCCAAAGTATTTCAAGTCAGAGTGGTCTGTTGCTCAGTTTCGTTTGCATGAAGGCTCCCAGTATGTTGTTGCTTTTGGTCACCAGAAGAATACAGTGGTAATCCTTGGAATGGATGGAAGCTTCTATAGATGCCAATTTGATCCAGCGACAGGAGGAGAGATGACACAGCTCGAATATCACAACTTTCTAATGCCTGAGGAAGCCTTCTAGCCGAATATTCTCACTAATTTCGGTTATGTCACGTGGGCTATACTTCTAAAAGGTGAAGGAGAAGAAAGATGACTCGTAGAAACCACTTGAGATAAATTCATTGTGAATATAACAGCATTTGAATGAGTGTAATATCAGCTTCCTGAACTTTCCTTATGAAATTGAGTTGGCGTTGACTGCATATGATGCATGACTAAAGTTTACTATTTAAGTTTAGATTCTTTTAAGAAAAGTCTGAAGGGAAAGTTACCCAAAATGCTTTTTTACTTCGCCCTTTGTTTAAAATGCCCCTATCTCTCAAAGTTTCAATAGTGTCCTTGACTCGTGGCAAAATTTGGAATGACGTGACAATTAGTGCTGAAGTTTGCTTTGATTAAGTTTCTAAAGAGAAGAAATGTGGGAGAAAATATCATTTTTAAAACGAGTGTCGGGATAGACAAGCGAG

Coding sequence (CDS)

ATGGAGACCCTCTCCGCCTTCCCCTCCCCTCCATGGCCCAACCCAAACCCAAGCCCTAATCCCAACCCCCCGCTTCCGGAGAACTCCGACCGCTTCGATTCCGATTCCGTTTCATCGGCCGAGCCCCCGGAGCCCAAGGTTCAGACCGTTCTGCCGGCGCCGGGCGACTCGCTCGCGTCGCTGCCCTCGCTGCTCCATCTCTCCTTCAATCAAGATCATGGCTGCTTTGCTGCGGGCACTGACCGGGGCTTTCGGATCTACAACTGCGATCCGTTCCGCGAGATTTTCAGGAGGGATTTCGATCGCGGGGGCGGCGTTGGCGTCGTGGAGATGCTTTTCCGGTGCAATATCTTGGCCCTTGTTGGCGGTGGGCCTGACCCTCAATACCCGCCTAATAAGGTCATGATTTGGGACGACCATCAGAGCCGATGCATTGGGGAGCTGTCGTTTCGCTCTGTTGTTCGTGGTGTGCGGCTGCAGAGGGACCGGATTATTGTGATTTTGGAGCAGAAGGTTTTCGTTTACAATTTTGCGGACTTGAAGCTGCTGCATCAGATCGAAACTATTGCGAACCCTAAGGGGCTTTGTGCGGTTTCCCAGTTGAGTACGTCGCTTGTGCTCGTTTGCCCCGGACTGCAGAAGGGGCAGGTTAGGGTTGAGCATTACGCCTCTCGACGGACCAAGTTCATAATGGCCCACGACTCGAGGATTGCTTGCTTTGCGCTTACGACCAATGGGCAGTTGCTTGCCACGGCGAGCAGCAAGGGAACTCTGGTTCGGATATTCAACACATTTGATGGAAATCTGCTTCAAGAGGTAAGGAGAGGCGCAGATAGAGCGGAGATCTACAGTTTGGCCTTTTCATCGACTGCCCAATGGCTGGCAGTCTCCAGTGACAAGGGAACCGTCCATGTATTCAGCCTTAAAGTCAATTCTGGATCTCTTGGAAATGATATGTCTCAGAATGATTCTTCTCTTTCTGTTGCACCAACTGGCTCCTCATTTTCCTTCATCAAAGGTGTGTTGCCAAAGTATTTCAAGTCAGAGTGGTCTGTTGCTCAGTTTCGTTTGCATGAAGGCTCCCAGTATGTTGTTGCTTTTGGTCACCAGAAGAATACAGTGGTAATCCTTGGAATGGATGGAAGCTTCTATAGATGCCAATTTGATCCAGCGACAGGAGGAGAGATGACACAGCTCGAATATCACAACTTTCTAATGCCTGAGGAAGCCTTCTAG

Protein sequence

METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF
Homology
BLAST of MC09g0628 vs. ExPASy Swiss-Prot
Match: Q93VB2 (Autophagy-related protein 18a OS=Arabidopsis thaliana OX=3702 GN=ATG18A PE=1 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 8.9e-173
Identity = 309/425 (72.71%), Postives = 339/425 (79.76%), Query Frame = 0

Query: 4   LSAFPSPPWPNPNPSPNPNPPL---------------PENSDRFDSDSVSSAEPPEPKVQ 63
           ++   S  WPNPNP+P+                    P++ D F S S++S EP +   Q
Sbjct: 1   MATVSSSSWPNPNPNPDSTSASDSDSTFPSHRDRVDEPDSLDSFSSMSLNSDEPNQTSNQ 60

Query: 64  TVLPAPGDSLASL--PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 123
           + L  P  +L  +  PS+LHLSFNQDH CFA GTDRGFRI NCDPFREIFRRDFDRGGGV
Sbjct: 61  SPLSPPTPNLPVMPPPSVLHLSFNQDHACFAVGTDRGFRILNCDPFREIFRRDFDRGGGV 120

Query: 124 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIV 183
            VVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQ RCIGELSFRS VR VRL+RDRIIV
Sbjct: 121 AVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLRRDRIIV 180

Query: 184 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 243
           +LEQK+FVYNF+DLKL+HQIETIANPKGLCAVSQ   S+VLVCPGLQKGQVR+EHYAS+R
Sbjct: 181 VLEQKIFVYNFSDLKLMHQIETIANPKGLCAVSQGVGSMVLVCPGLQKGQVRIEHYASKR 240

Query: 244 TKFIMAHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 303
           TKF+MAHDSRIACFALT +G LLATASSKGTLVRIFNT DG L QEVRRGADRAEIYSLA
Sbjct: 241 TKFVMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRRGADRAEIYSLA 300

Query: 304 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYF 363
           FSS AQWLAVSSDKGTVHVF LKVNSGS   D S+     + +   SS S  KGVLP+YF
Sbjct: 301 FSSNAQWLAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKGVLPRYF 360

Query: 364 KSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLM 412
            SEWSVAQFRL EG+QY+ AFGHQKNTVVILGMDGSFYRCQFDP  GGEM+QLEYHN L 
Sbjct: 361 SSEWSVAQFRLVEGTQYIAAFGHQKNTVVILGMDGSFYRCQFDPVNGGEMSQLEYHNCLK 420

BLAST of MC09g0628 vs. ExPASy Swiss-Prot
Match: Q0WPK3 (Autophagy-related protein 18d OS=Arabidopsis thaliana OX=3702 GN=ATG18D PE=2 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.4e-124
Identity = 216/352 (61.36%), Postives = 268/352 (76.14%), Query Frame = 0

Query: 64  LLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGG 123
           L+ +S+NQD+ CFAAGT  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILALVGG
Sbjct: 36  LVSVSWNQDYSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILALVGG 95

Query: 124 GPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADLKLL 183
           GP+ QYP NKV+IWDDHQ RCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL+LL
Sbjct: 96  GPNSQYPSNKVLIWDDHQGRCISEFTFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDLRLL 155

Query: 184 HQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACFALT 243
           HQIE +ANP+GLC +S    + VL CPG+++G+VRVEH+     + I AHDS IAC  LT
Sbjct: 156 HQIENMANPRGLCCLSHHMNTSVLACPGIRRGEVRVEHFGLNMVQIINAHDSNIACMTLT 215

Query: 244 TNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTV 303
            +G LLATAS+KGTL+RIFNT DG  LQEVRRG DRA+IYS+A S   QWLAVSSDKGTV
Sbjct: 216 LDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDKGTV 275

Query: 304 HVFSLKV-----NSGSLGNDMSQNDSSLSVAPT-----GSSFSFIKGVLPKYFKSEWSVA 363
           H+FSL+V     ++ S  ++ S N     V+P      GSS SF++GVLPKYF SEWS +
Sbjct: 276 HIFSLRVRVIGEDAYSTEHETSSNSLQPLVSPASGANPGSSLSFLRGVLPKYFSSEWSFS 335

Query: 364 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 406
           QF + E +QY  AFG Q NT+ I+G+DGSFYRC FDP  GGEMTQLE+ +FL
Sbjct: 336 QFHVPEVTQYFAAFGAQ-NTIAIIGLDGSFYRCNFDPVNGGEMTQLEHFHFL 385

BLAST of MC09g0628 vs. ExPASy Swiss-Prot
Match: Q8GYD7 (Autophagy-related protein 18c OS=Arabidopsis thaliana OX=3702 GN=ATG18C PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.9e-122
Identity = 218/358 (60.89%), Postives = 262/358 (73.18%), Query Frame = 0

Query: 64  LLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGG 123
           L+ + +NQD  CFAAGT  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILALVGG
Sbjct: 32  LVSVCWNQDSSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILALVGG 91

Query: 124 GPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADLKLL 183
           GP+ QYP +KV+IWDDHQSRCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL+LL
Sbjct: 92  GPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDLRLL 151

Query: 184 HQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACFALT 243
           HQIET ANP+GLC +S  S + VL CPGL +G++RVEH+     + I AHDS IAC  LT
Sbjct: 152 HQIETQANPRGLCCLSHHSNTSVLACPGLNRGEIRVEHFGLNMVQIINAHDSSIACMTLT 211

Query: 244 TNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTV 303
            +G LLATAS+KGTL+RIFNT DG  LQEVRRG DRA+IYS+A S   QWLAVSSDKGTV
Sbjct: 212 LDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDKGTV 271

Query: 304 HVFSLKV-----------NSGSLGNDMSQNDSSLSVAPT-----GSSFSFIKGVLPKYFK 363
           H+FSL+V           N   L      N     V+PT     GSS SF++GVLPKYF 
Sbjct: 272 HIFSLRVRVVGEDSYSTENGALLTQQNYSNSLQGLVSPTIGTNPGSSLSFMRGVLPKYFS 331

Query: 364 SEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 406
           SEWS AQF + E +Q+  AFG   NTV I+GMDGSFYRC FDP  GGEM QLEY +F+
Sbjct: 332 SEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSFDPVNGGEMGQLEYIHFM 387

BLAST of MC09g0628 vs. ExPASy Swiss-Prot
Match: Q9FHK8 (Autophagy-related protein 18e OS=Arabidopsis thaliana OX=3702 GN=ATG18E PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.1e-85
Identity = 166/359 (46.24%), Postives = 230/359 (64.07%), Query Frame = 0

Query: 53  APGDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEML 112
           +P D   S   +L +++NQ    F  GT+ GF +Y+C P  +          G  V EML
Sbjct: 22  SPRDEAKSDLKVLSVAWNQVCSGFIVGTNHGFNVYSCKPMIKKSISRAPHESGFKVAEML 81

Query: 113 FRCNILALVGGG-PDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQK 172
           F  N+ A VG G  + +YPPNKV +WDD+++ C+ EL+F+S V  V+L R+ ++V+L+Q 
Sbjct: 82  FLSNLFAFVGNGYNNSEYPPNKVFVWDDYRNCCLSELTFKSEVIAVKLAREHVVVVLKQN 141

Query: 173 VFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIM 232
           ++VY F +LK+   IET+ NPKGLC V+ + +  VL CPG   GQV+V        KFI 
Sbjct: 142 IYVYTFNNLKVDRVIETLMNPKGLCCVTHVESKAVLACPGFHPGQVQVHDLRWNVIKFIK 201

Query: 233 AHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTA 292
           AHDS IAC  LT +G LLATAS+KGTL+RIFN  DG LLQE RRG +RAEIY++A SS  
Sbjct: 202 AHDSAIACMTLTLDGSLLATASTKGTLIRIFNAVDGTLLQEFRRGVERAEIYNVAISSNL 261

Query: 293 QWLAVSSDKGTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKY-FKSEW 352
           +W+A SS+KGT+HVF L+                LS  P  SS SFI+ +LPKY +++E 
Sbjct: 262 KWVAASSEKGTLHVFRLR-------------PDILSFDPASSS-SFIRVILPKYLYENER 321

Query: 353 SVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEE 410
           S AQF L   ++++V FG  +NTV+++G+DGSF RC+FD A GG+M +LE+  F   +E
Sbjct: 322 SFAQFSLPASTKFIVGFG-SENTVLLVGIDGSFRRCKFDHADGGQMVELEHKYFFSLQE 365

BLAST of MC09g0628 vs. ExPASy Swiss-Prot
Match: Q640T2 (WD repeat domain phosphoinositide-interacting protein 3 OS=Xenopus tropicalis OX=8364 GN=wdr45b PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.2e-78
Identity = 159/345 (46.09%), Postives = 218/345 (63.19%), Query Frame = 0

Query: 64  LLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGG 123
           LL+  FNQDHGCFA G + GFR+YN DP +E  +++F   GGVG VEMLFRCN LALVGG
Sbjct: 13  LLYAGFNQDHGCFACGMENGFRVYNTDPLKEKEKQEF-LEGGVGYVEMLFRCNYLALVGG 72

Query: 124 GPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFA-DLKL 183
           G  P+YPPNKVMIWDD + + + E+ F + V+ V+L+RDRI+V+L+  + V+ F  +   
Sbjct: 73  GKKPKYPPNKVMIWDDLKKKTVIEIEFSTEVKAVKLRRDRIVVVLDSMIKVFTFTHNPHQ 132

Query: 184 LHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYAS--RRTKFIMAHDSRIACF 243
           LH  ET  NPKGLC +   S + +L  PG   G V++   AS  +    I AH+  ++C 
Sbjct: 133 LHVFETCYNPKGLCVLCPNSNNSLLAFPGAHTGHVQIVDLASTEKPPVDIPAHEGILSCI 192

Query: 244 ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 303
           AL   G  +ATAS KGTL+RIF+T  G+L+QE+RRG+  A IY + F+  A  + VSSD 
Sbjct: 193 ALNLQGTRIATASEKGTLIRIFDTSSGHLIQELRRGSQAANIYCINFNEDASLICVSSDH 252

Query: 304 GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG 363
           GTVH+F+          D  +N  S     + +S SF    LPKYF S+WS ++F++  G
Sbjct: 253 GTVHIFA--------AEDPKRNKQS-----SLASASF----LPKYFSSKWSFSKFQVPSG 312

Query: 364 SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 406
           S  V AFG + N+V+ +  DGS+Y+ QF+P   GE T+  Y  FL
Sbjct: 313 SPCVCAFGTEPNSVIAICADGSYYKFQFNPK--GECTRDVYAQFL 337

BLAST of MC09g0628 vs. NCBI nr
Match: XP_022147929.1 (autophagy-related protein 18a [Momordica charantia])

HSP 1 Score: 823 bits (2126), Expect = 6.87e-301
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS 60
           METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS
Sbjct: 1   METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS 60

Query: 61  LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL 120
           LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL
Sbjct: 61  LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL 120

Query: 121 VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL 180
           VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL
Sbjct: 121 VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL 180

Query: 181 KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF 240
           KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF
Sbjct: 181 KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF 240

Query: 241 ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 300
           ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK
Sbjct: 241 ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 300

Query: 301 GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG 360
           GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG
Sbjct: 301 GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG 360

Query: 361 SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF
Sbjct: 361 SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411

BLAST of MC09g0628 vs. NCBI nr
Match: KAA0068022.1 (autophagy-related protein 18a [Cucumis melo var. makuwa] >TYK18106.1 autophagy-related protein 18a [Cucumis melo var. makuwa])

HSP 1 Score: 745 bits (1924), Expect = 4.04e-270
Identity = 381/416 (91.59%), Postives = 389/416 (93.51%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLP----ENSDRFDSDSVSSAEPPEPKVQTVLPAPGD 60
           METLSAFPSPPWPNPNP+PNPNPP       +S+RFDSDSVSS +P      T L    +
Sbjct: 1   METLSAFPSPPWPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP------TGLSPAVE 60

Query: 61  SLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCN 120
             AS PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCN
Sbjct: 61  PPASQPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCN 120

Query: 121 ILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYN 180
           ILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIVILEQKVFVYN
Sbjct: 121 ILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYN 180

Query: 181 FADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSR 240
           FADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSR
Sbjct: 181 FADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSR 240

Query: 241 IACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAV 300
           IACFALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAV
Sbjct: 241 IACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAV 300

Query: 301 SSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQF 360
           SSDKGTVHVFSLKVNSGSLGNDM+ QNDSSLSVAP+GSSFSFIKGVLPKYFKSEWSVAQF
Sbjct: 301 SSDKGTVHVFSLKVNSGSLGNDMTHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVAQF 360

Query: 361 RLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           RLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFLMPEEAF
Sbjct: 361 RLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 410

BLAST of MC09g0628 vs. NCBI nr
Match: XP_038876739.1 (autophagy-related protein 18a [Benincasa hispida])

HSP 1 Score: 744 bits (1920), Expect = 1.64e-269
Identity = 382/412 (92.72%), Postives = 389/412 (94.42%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS 60
           METLSAFPSPPWPNPN +PNP PP   +S+RF SDSVSSA PP+    T L    +SLA 
Sbjct: 1   METLSAFPSPPWPNPNSNPNP-PPAIADSERFVSDSVSSA-PPQTTEPTGLSPAVESLAL 60

Query: 61  LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL 120
            PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL
Sbjct: 61  QPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL 120

Query: 121 VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL 180
           VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIVILEQKVFVYNFADL
Sbjct: 121 VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYNFADL 180

Query: 181 KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF 240
           KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF
Sbjct: 181 KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF 240

Query: 241 ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 300
           ALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK
Sbjct: 241 ALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 300

Query: 301 GTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHE 360
           GTVHVFSLKVNSGSLGNDMS QNDSSLSVAP+GSSFSFIKGVLPKYFKSEWSVAQFRLHE
Sbjct: 301 GTVHVFSLKVNSGSLGNDMSHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVAQFRLHE 360

Query: 361 GSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           GSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFLMPEEAF
Sbjct: 361 GSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 410

BLAST of MC09g0628 vs. NCBI nr
Match: XP_008451609.1 (PREDICTED: autophagy-related protein 18a [Cucumis melo])

HSP 1 Score: 742 bits (1916), Expect = 7.21e-269
Identity = 380/418 (90.91%), Postives = 389/418 (93.06%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLPE------NSDRFDSDSVSSAEPPEPKVQTVLPAP 60
           METLSAFPSPPWPNPNP+PNPNP  P       +S+RFDSDSVSS +P      T L   
Sbjct: 1   METLSAFPSPPWPNPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP------TGLSPA 60

Query: 61  GDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR 120
            +  AS PSLLHLSFNQDHGCFAAG+DRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR
Sbjct: 61  VEPPASQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR 120

Query: 121 CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFV 180
           CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIVILEQKVFV
Sbjct: 121 CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180

Query: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240
           YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD
Sbjct: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240

Query: 241 SRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300
           SRIACFALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL
Sbjct: 241 SRIACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300

Query: 301 AVSSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVA 360
           AVSSDKGTVHVFSLKVNSGSLGNDM+ QNDSSLSVAP+GSSFSFIKGVLPKYFKSEWSVA
Sbjct: 301 AVSSDKGTVHVFSLKVNSGSLGNDMTHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVA 360

Query: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFLMPEEAF
Sbjct: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 412

BLAST of MC09g0628 vs. NCBI nr
Match: XP_004136045.1 (autophagy-related protein 18a [Cucumis sativus] >KGN44912.1 hypothetical protein Csa_016023 [Cucumis sativus])

HSP 1 Score: 738 bits (1906), Expect = 3.25e-267
Identity = 382/426 (89.67%), Postives = 389/426 (91.31%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPS----------PNPNPPLP----ENSDRFDSDSVSSAEPPEPK 60
           METLSAFPSPPWPNPNP+          PNPNPP       +S+RFDSDSVSS +P    
Sbjct: 1   METLSAFPSPPWPNPNPNSNPSPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP---- 60

Query: 61  VQTVLPAPGDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 120
             T L    +  AS PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV
Sbjct: 61  --TGLSPAVEPPASQPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 120

Query: 121 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIV 180
           GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIV
Sbjct: 121 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIV 180

Query: 181 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 240
           ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR
Sbjct: 181 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 240

Query: 241 TKFIMAHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 300
           TKFIMAHDSRIACFALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA
Sbjct: 241 TKFIMAHDSRIACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 300

Query: 301 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKY 360
           FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMS QNDSSLSVAP+GSSFSFIKGVLPKY
Sbjct: 301 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMSHQNDSSLSVAPSGSSFSFIKGVLPKY 360

Query: 361 FKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 411
           FKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFL
Sbjct: 361 FKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFL 420

BLAST of MC09g0628 vs. ExPASy TrEMBL
Match: A0A6J1D2H1 (autophagy-related protein 18a OS=Momordica charantia OX=3673 GN=LOC111016737 PE=4 SV=1)

HSP 1 Score: 823 bits (2126), Expect = 3.33e-301
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS 60
           METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS
Sbjct: 1   METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEPPEPKVQTVLPAPGDSLAS 60

Query: 61  LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL 120
           LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL
Sbjct: 61  LPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILAL 120

Query: 121 VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL 180
           VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL
Sbjct: 121 VGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADL 180

Query: 181 KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF 240
           KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF
Sbjct: 181 KLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACF 240

Query: 241 ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 300
           ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK
Sbjct: 241 ALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDK 300

Query: 301 GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG 360
           GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG
Sbjct: 301 GTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQFRLHEG 360

Query: 361 SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF
Sbjct: 361 SQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411

BLAST of MC09g0628 vs. ExPASy TrEMBL
Match: A0A5A7VI57 (Autophagy-related protein 18a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00280 PE=4 SV=1)

HSP 1 Score: 745 bits (1924), Expect = 1.96e-270
Identity = 381/416 (91.59%), Postives = 389/416 (93.51%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLP----ENSDRFDSDSVSSAEPPEPKVQTVLPAPGD 60
           METLSAFPSPPWPNPNP+PNPNPP       +S+RFDSDSVSS +P      T L    +
Sbjct: 1   METLSAFPSPPWPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP------TGLSPAVE 60

Query: 61  SLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCN 120
             AS PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCN
Sbjct: 61  PPASQPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCN 120

Query: 121 ILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYN 180
           ILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIVILEQKVFVYN
Sbjct: 121 ILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFVYN 180

Query: 181 FADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSR 240
           FADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSR
Sbjct: 181 FADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSR 240

Query: 241 IACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAV 300
           IACFALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAV
Sbjct: 241 IACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAV 300

Query: 301 SSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVAQF 360
           SSDKGTVHVFSLKVNSGSLGNDM+ QNDSSLSVAP+GSSFSFIKGVLPKYFKSEWSVAQF
Sbjct: 301 SSDKGTVHVFSLKVNSGSLGNDMTHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVAQF 360

Query: 361 RLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           RLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFLMPEEAF
Sbjct: 361 RLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 410

BLAST of MC09g0628 vs. ExPASy TrEMBL
Match: A0A1S3BRA9 (autophagy-related protein 18a OS=Cucumis melo OX=3656 GN=LOC103492842 PE=4 SV=1)

HSP 1 Score: 742 bits (1916), Expect = 3.49e-269
Identity = 380/418 (90.91%), Postives = 389/418 (93.06%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLPE------NSDRFDSDSVSSAEPPEPKVQTVLPAP 60
           METLSAFPSPPWPNPNP+PNPNP  P       +S+RFDSDSVSS +P      T L   
Sbjct: 1   METLSAFPSPPWPNPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP------TGLSPA 60

Query: 61  GDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR 120
            +  AS PSLLHLSFNQDHGCFAAG+DRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR
Sbjct: 61  VEPPASQPSLLHLSFNQDHGCFAAGSDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR 120

Query: 121 CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFV 180
           CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIVILEQKVFV
Sbjct: 121 CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180

Query: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240
           YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD
Sbjct: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240

Query: 241 SRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300
           SRIACFALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL
Sbjct: 241 SRIACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300

Query: 301 AVSSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVA 360
           AVSSDKGTVHVFSLKVNSGSLGNDM+ QNDSSLSVAP+GSSFSFIKGVLPKYFKSEWSVA
Sbjct: 301 AVSSDKGTVHVFSLKVNSGSLGNDMTHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVA 360

Query: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFLMPEEAF
Sbjct: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 412

BLAST of MC09g0628 vs. ExPASy TrEMBL
Match: A0A0A0K960 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G395790 PE=4 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 1.57e-267
Identity = 382/426 (89.67%), Postives = 389/426 (91.31%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPS----------PNPNPPLP----ENSDRFDSDSVSSAEPPEPK 60
           METLSAFPSPPWPNPNP+          PNPNPP       +S+RFDSDSVSS +P    
Sbjct: 1   METLSAFPSPPWPNPNPNSNPSPNPNPNPNPNPPSAVADSSSSERFDSDSVSSVDP---- 60

Query: 61  VQTVLPAPGDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 120
             T L    +  AS PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV
Sbjct: 61  --TGLSPAVEPPASQPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 120

Query: 121 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIV 180
           GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIV
Sbjct: 121 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIV 180

Query: 181 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 240
           ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR
Sbjct: 181 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 240

Query: 241 TKFIMAHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 300
           TKFIMAHDSRIACFALTTNGQLLATAS+KGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA
Sbjct: 241 TKFIMAHDSRIACFALTTNGQLLATASTKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 300

Query: 301 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKY 360
           FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMS QNDSSLSVAP+GSSFSFIKGVLPKY
Sbjct: 301 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMSHQNDSSLSVAPSGSSFSFIKGVLPKY 360

Query: 361 FKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 411
           FKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFL
Sbjct: 361 FKSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFL 420

BLAST of MC09g0628 vs. ExPASy TrEMBL
Match: A0A6J1GPZ4 (autophagy-related protein 18a-like OS=Cucurbita moschata OX=3662 GN=LOC111456412 PE=4 SV=1)

HSP 1 Score: 731 bits (1887), Expect = 1.02e-264
Identity = 376/418 (89.95%), Postives = 383/418 (91.63%), Query Frame = 0

Query: 1   METLSAFPSPPWPNPNPSPNPNPPLPENSDRFDSDSVSSAEP------PEPKVQTVLPAP 60
           METLSAFPSPPWPNP P+P   PP    S+RFDSDSVSS  P      P+    TV    
Sbjct: 1   METLSAFPSPPWPNPEPNP---PPAVAESERFDSDSVSSEPPQLLPQLPQTVETTVSSTA 60

Query: 61  GDSLASLPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFR 120
            +  AS PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGG+GVVEMLFR
Sbjct: 61  VEPAASQPSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGIGVVEMLFR 120

Query: 121 CNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFV 180
           CNILALVGGGPDPQYP NKVMIWDDHQSRCIGELSFRS VRGVRLQRDRIIVILEQKVFV
Sbjct: 121 CNILALVGGGPDPQYPCNKVMIWDDHQSRCIGELSFRSAVRGVRLQRDRIIVILEQKVFV 180

Query: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240
           YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD
Sbjct: 181 YNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHD 240

Query: 241 SRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300
           SRIACFALTTNGQLLATAS+KGTLVRIFNT DGNLLQEVRRGADRAEIYSLAFSSTAQWL
Sbjct: 241 SRIACFALTTNGQLLATASTKGTLVRIFNTVDGNLLQEVRRGADRAEIYSLAFSSTAQWL 300

Query: 301 AVSSDKGTVHVFSLKVNSGSLGNDMS-QNDSSLSVAPTGSSFSFIKGVLPKYFKSEWSVA 360
           AVSSDKGTVHVFSLKVNSGSLGNDMS QNDSSLSVAP+GSSFSFIKGVLPKYFKSEWSVA
Sbjct: 301 AVSSDKGTVHVFSLKVNSGSLGNDMSHQNDSSLSVAPSGSSFSFIKGVLPKYFKSEWSVA 360

Query: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLMPEEAF 411
           QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFD   GGEMTQLEYHNFLMPEEAF
Sbjct: 361 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDSVNGGEMTQLEYHNFLMPEEAF 415

BLAST of MC09g0628 vs. TAIR 10
Match: AT3G62770.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 607.8 bits (1566), Expect = 6.3e-174
Identity = 309/425 (72.71%), Postives = 339/425 (79.76%), Query Frame = 0

Query: 4   LSAFPSPPWPNPNPSPNPNPPL---------------PENSDRFDSDSVSSAEPPEPKVQ 63
           ++   S  WPNPNP+P+                    P++ D F S S++S EP +   Q
Sbjct: 1   MATVSSSSWPNPNPNPDSTSASDSDSTFPSHRDRVDEPDSLDSFSSMSLNSDEPNQTSNQ 60

Query: 64  TVLPAPGDSLASL--PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 123
           + L  P  +L  +  PS+LHLSFNQDH CFA GTDRGFRI NCDPFREIFRRDFDRGGGV
Sbjct: 61  SPLSPPTPNLPVMPPPSVLHLSFNQDHACFAVGTDRGFRILNCDPFREIFRRDFDRGGGV 120

Query: 124 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIV 183
            VVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQ RCIGELSFRS VR VRL+RDRIIV
Sbjct: 121 AVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLRRDRIIV 180

Query: 184 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 243
           +LEQK+FVYNF+DLKL+HQIETIANPKGLCAVSQ   S+VLVCPGLQKGQVR+EHYAS+R
Sbjct: 181 VLEQKIFVYNFSDLKLMHQIETIANPKGLCAVSQGVGSMVLVCPGLQKGQVRIEHYASKR 240

Query: 244 TKFIMAHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 303
           TKF+MAHDSRIACFALT +G LLATASSKGTLVRIFNT DG L QEVRRGADRAEIYSLA
Sbjct: 241 TKFVMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRRGADRAEIYSLA 300

Query: 304 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYF 363
           FSS AQWLAVSSDKGTVHVF LKVNSGS   D S+     + +   SS S  KGVLP+YF
Sbjct: 301 FSSNAQWLAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKGVLPRYF 360

Query: 364 KSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFLM 412
            SEWSVAQFRL EG+QY+ AFGHQKNTVVILGMDGSFYRCQFDP  GGEM+QLEYHN L 
Sbjct: 361 SSEWSVAQFRLVEGTQYIAAFGHQKNTVVILGMDGSFYRCQFDPVNGGEMSQLEYHNCLK 420

BLAST of MC09g0628 vs. TAIR 10
Match: AT3G62770.3 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 557.8 bits (1436), Expect = 7.5e-159
Identity = 287/395 (72.66%), Postives = 316/395 (80.00%), Query Frame = 0

Query: 4   LSAFPSPPWPNPNPSPNPNPPL---------------PENSDRFDSDSVSSAEPPEPKVQ 63
           ++   S  WPNPNP+P+                    P++ D F S S++S EP +   Q
Sbjct: 1   MATVSSSSWPNPNPNPDSTSASDSDSTFPSHRDRVDEPDSLDSFSSMSLNSDEPNQTSNQ 60

Query: 64  TVLPAPGDSLASL--PSLLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGV 123
           + L  P  +L  +  PS+LHLSFNQDH CFA GTDRGFRI NCDPFREIFRRDFDRGGGV
Sbjct: 61  SPLSPPTPNLPVMPPPSVLHLSFNQDHACFAVGTDRGFRILNCDPFREIFRRDFDRGGGV 120

Query: 124 GVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIV 183
            VVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQ RCIGELSFRS VR VRL+RDRIIV
Sbjct: 121 AVVEMLFRCNILALVGGGPDPQYPPNKVMIWDDHQGRCIGELSFRSDVRSVRLRRDRIIV 180

Query: 184 ILEQKVFVYNFADLKLLHQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRR 243
           +LEQK+FVYNF+DLKL+HQIETIANPKGLCAVSQ   S+VLVCPGLQKGQVR+EHYAS+R
Sbjct: 181 VLEQKIFVYNFSDLKLMHQIETIANPKGLCAVSQGVGSMVLVCPGLQKGQVRIEHYASKR 240

Query: 244 TKFIMAHDSRIACFALTTNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLA 303
           TKF+MAHDSRIACFALT +G LLATASSKGTLVRIFNT DG L QEVRRGADRAEIYSLA
Sbjct: 241 TKFVMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRRGADRAEIYSLA 300

Query: 304 FSSTAQWLAVSSDKGTVHVFSLKVNSGSLGNDMSQNDSSLSVAPTGSSFSFIKGVLPKYF 363
           FSS AQWLAVSSDKGTVHVF LKVNSGS   D S+     + +   SS S  KGVLP+YF
Sbjct: 301 FSSNAQWLAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKGVLPRYF 360

Query: 364 KSEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDG 382
            SEWSVAQFRL EG+QY+ AFGHQKNTVVILGMDG
Sbjct: 361 SSEWSVAQFRLVEGTQYIAAFGHQKNTVVILGMDG 395

BLAST of MC09g0628 vs. TAIR 10
Match: AT3G56440.1 (homolog of yeast autophagy 18 (ATG18) D )

HSP 1 Score: 446.4 bits (1147), Expect = 2.4e-125
Identity = 216/352 (61.36%), Postives = 268/352 (76.14%), Query Frame = 0

Query: 64  LLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGG 123
           L+ +S+NQD+ CFAAGT  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILALVGG
Sbjct: 36  LVSVSWNQDYSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILALVGG 95

Query: 124 GPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADLKLL 183
           GP+ QYP NKV+IWDDHQ RCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL+LL
Sbjct: 96  GPNSQYPSNKVLIWDDHQGRCISEFTFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDLRLL 155

Query: 184 HQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACFALT 243
           HQIE +ANP+GLC +S    + VL CPG+++G+VRVEH+     + I AHDS IAC  LT
Sbjct: 156 HQIENMANPRGLCCLSHHMNTSVLACPGIRRGEVRVEHFGLNMVQIINAHDSNIACMTLT 215

Query: 244 TNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTV 303
            +G LLATAS+KGTL+RIFNT DG  LQEVRRG DRA+IYS+A S   QWLAVSSDKGTV
Sbjct: 216 LDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDKGTV 275

Query: 304 HVFSLKV-----NSGSLGNDMSQNDSSLSVAPT-----GSSFSFIKGVLPKYFKSEWSVA 363
           H+FSL+V     ++ S  ++ S N     V+P      GSS SF++GVLPKYF SEWS +
Sbjct: 276 HIFSLRVRVIGEDAYSTEHETSSNSLQPLVSPASGANPGSSLSFLRGVLPKYFSSEWSFS 335

Query: 364 QFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 406
           QF + E +QY  AFG Q NT+ I+G+DGSFYRC FDP  GGEMTQLE+ +FL
Sbjct: 336 QFHVPEVTQYFAAFGAQ-NTIAIIGLDGSFYRCNFDPVNGGEMTQLEHFHFL 385

BLAST of MC09g0628 vs. TAIR 10
Match: AT2G40810.1 (homolog of yeast autophagy 18C )

HSP 1 Score: 440.7 bits (1132), Expect = 1.3e-123
Identity = 218/358 (60.89%), Postives = 262/358 (73.18%), Query Frame = 0

Query: 64  LLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGG 123
           L+ + +NQD  CFAAGT  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILALVGG
Sbjct: 32  LVSVCWNQDSSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILALVGG 91

Query: 124 GPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADLKLL 183
           GP+ QYP +KV+IWDDHQSRCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL+LL
Sbjct: 92  GPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDLRLL 151

Query: 184 HQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACFALT 243
           HQIET ANP+GLC +S  S + VL CPGL +G++RVEH+     + I AHDS IAC  LT
Sbjct: 152 HQIETQANPRGLCCLSHHSNTSVLACPGLNRGEIRVEHFGLNMVQIINAHDSSIACMTLT 211

Query: 244 TNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTV 303
            +G LLATAS+KGTL+RIFNT DG  LQEVRRG DRA+IYS+A S   QWLAVSSDKGTV
Sbjct: 212 LDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDKGTV 271

Query: 304 HVFSLKV-----------NSGSLGNDMSQNDSSLSVAPT-----GSSFSFIKGVLPKYFK 363
           H+FSL+V           N   L      N     V+PT     GSS SF++GVLPKYF 
Sbjct: 272 HIFSLRVRVVGEDSYSTENGALLTQQNYSNSLQGLVSPTIGTNPGSSLSFMRGVLPKYFS 331

Query: 364 SEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 406
           SEWS AQF + E +Q+  AFG   NTV I+GMDGSFYRC FDP  GGEM QLEY +F+
Sbjct: 332 SEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSFDPVNGGEMGQLEYIHFM 387

BLAST of MC09g0628 vs. TAIR 10
Match: AT2G40810.2 (homolog of yeast autophagy 18C )

HSP 1 Score: 440.7 bits (1132), Expect = 1.3e-123
Identity = 218/358 (60.89%), Postives = 262/358 (73.18%), Query Frame = 0

Query: 64  LLHLSFNQDHGCFAAGTDRGFRIYNCDPFREIFRRDFDRGGGVGVVEMLFRCNILALVGG 123
           L+ + +NQD  CFAAGT  GFRIYNC+PF+E FRR+  + GG  +VEMLFR NILALVGG
Sbjct: 32  LVSVCWNQDSSCFAAGTSHGFRIYNCEPFKETFRREL-KDGGFKIVEMLFRSNILALVGG 91

Query: 124 GPDPQYPPNKVMIWDDHQSRCIGELSFRSVVRGVRLQRDRIIVILEQKVFVYNFADLKLL 183
           GP+ QYP +KV+IWDDHQSRCI E +FRS +R V+L+RDRI+V+LE K++VYNF DL+LL
Sbjct: 92  GPNSQYPSSKVLIWDDHQSRCISEFAFRSEIRAVKLRRDRIVVVLEHKIYVYNFMDLRLL 151

Query: 184 HQIETIANPKGLCAVSQLSTSLVLVCPGLQKGQVRVEHYASRRTKFIMAHDSRIACFALT 243
           HQIET ANP+GLC +S  S + VL CPGL +G++RVEH+     + I AHDS IAC  LT
Sbjct: 152 HQIETQANPRGLCCLSHHSNTSVLACPGLNRGEIRVEHFGLNMVQIINAHDSSIACMTLT 211

Query: 244 TNGQLLATASSKGTLVRIFNTFDGNLLQEVRRGADRAEIYSLAFSSTAQWLAVSSDKGTV 303
            +G LLATAS+KGTL+RIFNT DG  LQEVRRG DRA+IYS+A S   QWLAVSSDKGTV
Sbjct: 212 LDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALSPNVQWLAVSSDKGTV 271

Query: 304 HVFSLKV-----------NSGSLGNDMSQNDSSLSVAPT-----GSSFSFIKGVLPKYFK 363
           H+FSL+V           N   L      N     V+PT     GSS SF++GVLPKYF 
Sbjct: 272 HIFSLRVRVVGEDSYSTENGALLTQQNYSNSLQGLVSPTIGTNPGSSLSFMRGVLPKYFS 331

Query: 364 SEWSVAQFRLHEGSQYVVAFGHQKNTVVILGMDGSFYRCQFDPATGGEMTQLEYHNFL 406
           SEWS AQF + E +Q+  AFG   NTV I+GMDGSFYRC FDP  GGEM QLEY +F+
Sbjct: 332 SEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSFDPVNGGEMGQLEYIHFM 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VB28.9e-17372.71Autophagy-related protein 18a OS=Arabidopsis thaliana OX=3702 GN=ATG18A PE=1 SV=... [more]
Q0WPK33.4e-12461.36Autophagy-related protein 18d OS=Arabidopsis thaliana OX=3702 GN=ATG18D PE=2 SV=... [more]
Q8GYD71.9e-12260.89Autophagy-related protein 18c OS=Arabidopsis thaliana OX=3702 GN=ATG18C PE=2 SV=... [more]
Q9FHK83.1e-8546.24Autophagy-related protein 18e OS=Arabidopsis thaliana OX=3702 GN=ATG18E PE=2 SV=... [more]
Q640T22.2e-7846.09WD repeat domain phosphoinositide-interacting protein 3 OS=Xenopus tropicalis OX... [more]
Match NameE-valueIdentityDescription
XP_022147929.16.87e-301100.00autophagy-related protein 18a [Momordica charantia][more]
KAA0068022.14.04e-27091.59autophagy-related protein 18a [Cucumis melo var. makuwa] >TYK18106.1 autophagy-r... [more]
XP_038876739.11.64e-26992.72autophagy-related protein 18a [Benincasa hispida][more]
XP_008451609.17.21e-26990.91PREDICTED: autophagy-related protein 18a [Cucumis melo][more]
XP_004136045.13.25e-26789.67autophagy-related protein 18a [Cucumis sativus] >KGN44912.1 hypothetical protein... [more]
Match NameE-valueIdentityDescription
A0A6J1D2H13.33e-301100.00autophagy-related protein 18a OS=Momordica charantia OX=3673 GN=LOC111016737 PE=... [more]
A0A5A7VI571.96e-27091.59Autophagy-related protein 18a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BRA93.49e-26990.91autophagy-related protein 18a OS=Cucumis melo OX=3656 GN=LOC103492842 PE=4 SV=1[more]
A0A0A0K9601.57e-26789.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G395790 PE=4 SV=1[more]
A0A6J1GPZ41.02e-26489.95autophagy-related protein 18a-like OS=Cucurbita moschata OX=3662 GN=LOC111456412... [more]
Match NameE-valueIdentityDescription
AT3G62770.16.3e-17472.71Transducin/WD40 repeat-like superfamily protein [more]
AT3G62770.37.5e-15972.66Transducin/WD40 repeat-like superfamily protein [more]
AT3G56440.12.4e-12561.36homolog of yeast autophagy 18 (ATG18) D [more]
AT2G40810.11.3e-12360.89homolog of yeast autophagy 18C [more]
AT2G40810.21.3e-12360.89homolog of yeast autophagy 18C [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 348..387
e-value: 290.0
score: 1.4
coord: 54..88
e-value: 24.0
score: 8.2
coord: 266..307
e-value: 0.018
score: 24.2
coord: 223..263
e-value: 0.094
score: 21.8
IPR001680WD40 repeatPFAMPF00400WD40coord: 276..306
e-value: 0.0057
score: 17.5
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 64..403
e-value: 1.8E-19
score: 72.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..26
NoneNo IPR availablePANTHERPTHR11227WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATEDcoord: 20..410
NoneNo IPR availablePANTHERPTHR11227:SF61BNAC06G18590D PROTEINcoord: 20..410
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 63..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0628.1MC09g0628.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0044804 autophagy of nucleus
biological_process GO:0006497 protein lipidation
biological_process GO:0034497 protein localization to phagophore assembly site
cellular_component GO:0005829 cytosol
cellular_component GO:0019898 extrinsic component of membrane
cellular_component GO:0034045 phagophore assembly site membrane
molecular_function GO:0080025 phosphatidylinositol-3,5-bisphosphate binding
molecular_function GO:0032266 phosphatidylinositol-3-phosphate binding
molecular_function GO:0005515 protein binding