MC09g0593 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0593
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationMC09: 5438213 .. 5441245 (+)
RNA-Seq ExpressionMC09g0593
SyntenyMC09g0593
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGAAATTCACAAATCACAGAAAAATCAAACTTCAAAAATCTCAACAATGGAGAACATCGAGCACACCGTCATCTCCACCAATGGCATAGATATGCACGTCGCCTCAATCGGCACCGGCTCCGATTCCATCCTCTTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCACCTCCTCTCCTTCTCCGACCTCGGCTACCGCGCCATCGCCCCCGATCTCCGCGGCTATGGCGACACCGACGCGCCCCCCTCCCCGGAGTCCTACACCCTCTTCCACGTCGTCGGCGACCTCATCGGACTCCTCGACCATTTCGACATCGATAGGGTTTTCCTCGTCGGCCACGATTGGGGGGCTCTCATCGCCTGGGCCTTCGCTCTGTTCCGCCCCGATAGAATCAAGGCTCTCGTTGCCTTGAGCGTCCCGTTCCCTCCGAGGAATCCGGAGAGAAACTCTGTTGAGATCTTGAGGGATCTGTTCGGGGATGGTTACTACGTTTGCAGATTTCAGGTGCGTTTCATCCATTTTTGTTCTGGTTGTCCTTTTGCCTGTTTTTGAATGATCTTGAATCGAATCGAAGCAAATGGTAGTTTCTTGCAATGGCTTTTTCTTGAATAATGTGTTCTTTGGATATATATATGGTTCTTGCTGCTTTGAATTCTTGAATCTTCTGCAAGTGAGTACTGCCAATTTGTTTCGATTGCTTTGGTTGCTGATTCTTTTGGTTCTTTTAGGGTAGAAGATTTGATGGCGTTTCTTATTTCTTTTTCTTGATTTCCGTTAAAACTTAAAATTAGTAAACAAATAAAACTATGTTTTGTAATTGATTCATTTTTTTCCTTGTTCTTTTTTATTGAAAGAAAAAGGGAGAATTATGTAGGATAATCTTTTCTTGTTTCTTCTTCTTTGAAGATTTTAATTTGAAAAATTAAGTTTTACATAAAAAGTAATTTTTTGAAAATAAAATTTTCAAAATTAGTGGGATATGTTTAATTTTTAAGATTCGTTATACCTTTTATGGGTTAGAAATGATAAAAAAGTGATTATATTTATGTTAGTGAACAACTCCAAACATAAAATAAAATAAGAAACTAAAAACTAGATTTTATTATTTCCATAATTCATATACAATATTGAAAAAGTTTTTAAAAAATGAGAAATAGAAAATAATTACCTAATGCTTGTGGGTCTAAGATTGGACGTTACACCTAAAAAAAATTAAATAGATATCCTATTTGTTAAGTTATGTTCGGACTCAGTTTGTATTTGTAAAAGAAAATAATAAAAAATAGGAAATGAGAATTAGGGACACTGCCAACCAACTCCTTATATAATTTAAATCTATTGTTGTCTCTTGATTGGGCCTTGGGAACATTAGTGGGATAAGGTGCAAGCATTGGGGTCATAATATCCCATTATTATCCATTCTTTGTTTTAAAGAATTAAATTTTTTTTGATTGAAATATATATCTTCCAACAAATGTTGTAATCTCTATCCCTATCCAAGAATGTTATTGAACTGAAAAAAAAGTAAAGAAACGGGGGACTATGGTTCAGATGTTCCTCCCCTCCCCTAGTAGTGTAAACCTACAAAACTTCTCAAACTACTGCTGATATTATGCTGCTGCAGGAACCAGGGGAGATGGAAGAAGATTTTGATTCCATGGATACAGAAGATATCTTTAAGATTTTCCTTTCAATTCGCGACCCGGCTCCTCCAATTATACCGAAAGAAGTAGGAATGAGAGGGTATCCAATTCCAGACAGCCTTCCCTGCTGGTTGTCTCAGGAAAGTTTAAGTTACTATGCTACCAAGTTCGGCAAGAAAGGCTTTACTGGAGGATTCAACTACTTTCGAGCTCTCCCGCTGTATGTTCTTCACTTCTTTCACTCCTGCAAAATAGAAAGGCATATTCCTTGTCACTTTTGCATAATGCCTGATGAGTTTCTCATTCCCTTTCACTGCTCTGCATCTTCTTTGTTGGTTCCCTTAGCCTTTGAATAACATGATTGTATTGCAGCTGCTAACCAATGAGTAAAATTTCCGAGTTTGCTCGGTTAAAGCATACGCCATGATCGAAAGAAATGTGGGAATTGTTTGGTTTGCTCTTGCTATGAATTTCTAATTCCTTCTCATAGCTATGCAGGTCCTTTGTTGGTTCACTTGAATTACAAGAACTTTTAATAACAAATTGTTTTGATGTTTGGTTCTATATGGTCCTTTGAGTACTGGTGCTTCCTCAAAAAGATATTAGGGTGTTTTTGCCTTTTTTTCGAAACACTATTTAGAGTGAAGTGCTTTTAGTCATGGAAAAAGCCCTAGCTTTTGAATAGCATGAGTGTAGCATAGCTAGTTGACGAGTAGAAAATACCGAGTTTGATCGGTTAAATCGAACGTCGAGATCAGCAGAAATGTGGGAATCCTTGGGCACAAAAAAGCTGATCAAATGAAACTCCTCTTGTACTATTTGATCTCTAATAGATCTGTTGAAAGATCATTACAAAGGATAGCCTCTTTGTTGCAGAAACTGGGAGCTGACAGCGCCGTGGAGCGGGTCGAAAGTCGAAGTACCGAGCAAGTTCATCACGGGGGATTTGGATATCACGTACAACATCCCGGGGGTGAAGGAGTACATTCACAAGGGCGGATTCGAAAACGACGTGGTGGGGCTGGAAGAGATAGTAGTGATCGAAGGAGCTGCTCACTTTGTGAACCAAGAAAGTCCAGAGGAGGTCACCAAACAGATTCATGAATTTATCCAGAAGTATTGAGCCTCCTTCCCCATTAGCAATACAAGTTGGTTGGCCTTTTGCCTTTGCTTTTGTTAAGCATCTTGATTTTTTTTTTTGCAAGGCTCTCACTGGCTACGTTTTGTGATTAGTACTGTATATAGTGTTCTACCATTTGGGGCAATTGAAATTTGGAAGTATATTTTGTAAATAAAGAGCTCTTTCTCAAGACGATATTCTCGGTTTCCAACTTTCAATTTTCAAAGATAAATAATATCTTAATCCGTAGATAGAGA

mRNA sequence

GAGAGAAATTCACAAATCACAGAAAAATCAAACTTCAAAAATCTCAACAATGGAGAACATCGAGCACACCGTCATCTCCACCAATGGCATAGATATGCACGTCGCCTCAATCGGCACCGGCTCCGATTCCATCCTCTTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCACCTCCTCTCCTTCTCCGACCTCGGCTACCGCGCCATCGCCCCCGATCTCCGCGGCTATGGCGACACCGACGCGCCCCCCTCCCCGGAGTCCTACACCCTCTTCCACGTCGTCGGCGACCTCATCGGACTCCTCGACCATTTCGACATCGATAGGGTTTTCCTCGTCGGCCACGATTGGGGGGCTCTCATCGCCTGGGCCTTCGCTCTGTTCCGCCCCGATAGAATCAAGGCTCTCGTTGCCTTGAGCGTCCCGTTCCCTCCGAGGAATCCGGAGAGAAACTCTGTTGAGATCTTGAGGGATCTGTTCGGGGATGGTTACTACGTTTGCAGATTTCAGGAACCAGGGGAGATGGAAGAAGATTTTGATTCCATGGATACAGAAGATATCTTTAAGATTTTCCTTTCAATTCGCGACCCGGCTCCTCCAATTATACCGAAAGAAGTAGGAATGAGAGGGTATCCAATTCCAGACAGCCTTCCCTGCTGGTTGTCTCAGGAAAGTTTAAGTTACTATGCTACCAAGTTCGGCAAGAAAGGCTTTACTGGAGGATTCAACTACTTTCGAGCTCTCCCGCTAAACTGGGAGCTGACAGCGCCGTGGAGCGGGTCGAAAGTCGAAGTACCGAGCAAGTTCATCACGGGGGATTTGGATATCACGTACAACATCCCGGGGGTGAAGGAGTACATTCACAAGGGCGGATTCGAAAACGACGTGGTGGGGCTGGAAGAGATAGTAGTGATCGAAGGAGCTGCTCACTTTGTGAACCAAGAAAGTCCAGAGGAGGTCACCAAACAGATTCATGAATTTATCCAGAAGTATTGAGCCTCCTTCCCCATTAGCAATACAAGTTGGTTGGCCTTTTGCCTTTGCTTTTGTTAAGCATCTTGATTTTTTTTTTTGCAAGGCTCTCACTGGCTACGTTTTGTGATTAGTACTGTATATAGTGTTCTACCATTTGGGGCAATTGAAATTTGGAAGTATATTTTGTAAATAAAGAGCTCTTTCTCAAGACGATATTCTCGGTTTCCAACTTTCAATTTTCAAAGATAAATAATATCTTAATCCGTAGATAGAGA

Coding sequence (CDS)

ATGGAGAACATCGAGCACACCGTCATCTCCACCAATGGCATAGATATGCACGTCGCCTCAATCGGCACCGGCTCCGATTCCATCCTCTTCCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCACCTCCTCTCCTTCTCCGACCTCGGCTACCGCGCCATCGCCCCCGATCTCCGCGGCTATGGCGACACCGACGCGCCCCCCTCCCCGGAGTCCTACACCCTCTTCCACGTCGTCGGCGACCTCATCGGACTCCTCGACCATTTCGACATCGATAGGGTTTTCCTCGTCGGCCACGATTGGGGGGCTCTCATCGCCTGGGCCTTCGCTCTGTTCCGCCCCGATAGAATCAAGGCTCTCGTTGCCTTGAGCGTCCCGTTCCCTCCGAGGAATCCGGAGAGAAACTCTGTTGAGATCTTGAGGGATCTGTTCGGGGATGGTTACTACGTTTGCAGATTTCAGGAACCAGGGGAGATGGAAGAAGATTTTGATTCCATGGATACAGAAGATATCTTTAAGATTTTCCTTTCAATTCGCGACCCGGCTCCTCCAATTATACCGAAAGAAGTAGGAATGAGAGGGTATCCAATTCCAGACAGCCTTCCCTGCTGGTTGTCTCAGGAAAGTTTAAGTTACTATGCTACCAAGTTCGGCAAGAAAGGCTTTACTGGAGGATTCAACTACTTTCGAGCTCTCCCGCTAAACTGGGAGCTGACAGCGCCGTGGAGCGGGTCGAAAGTCGAAGTACCGAGCAAGTTCATCACGGGGGATTTGGATATCACGTACAACATCCCGGGGGTGAAGGAGTACATTCACAAGGGCGGATTCGAAAACGACGTGGTGGGGCTGGAAGAGATAGTAGTGATCGAAGGAGCTGCTCACTTTGTGAACCAAGAAAGTCCAGAGGAGGTCACCAAACAGATTCATGAATTTATCCAGAAGTATTGA

Protein sequence

MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLRGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIKALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSIRDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQESPEEVTKQIHEFIQKY
Homology
BLAST of MC09g0593 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 9.9e-55
Identity = 115/316 (36.39%), Postives = 167/316 (52.85%), Query Frame = 0

Query: 8   VISTNGIDMHVASIG-TGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLRGYGDT 67
           ++ TNG+ + V   G  G+  ++  HGFPEL YSWRH + + +D GY  +APD RGYG +
Sbjct: 8   LVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGS 67

Query: 68  DAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIKALVALS 127
             P + E+Y +  +  DL+GLLD    +R   VGHDWGA++ W   L   DR+ A+ ALS
Sbjct: 68  SRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALS 127

Query: 128 VPFPPRNPERNSVEILRDLFGDG-YYVCRFQEPGEMEEDFDSMDTEDIFKIFLSIRDPAP 187
           VP  PR  +    +  R  FG+  +Y+  FQEPG  + + +      + ++   +R P  
Sbjct: 128 VPALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGD 187

Query: 188 PIIPKEV---GMRGY----PIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNW 247
                 +   G  G+    P P  LP W+SQE L +Y  +F + GFTGG N++R    NW
Sbjct: 188 QSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNW 247

Query: 248 ELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFV 307
           E TA  +G  + VPS FI G  D       V  +         + G    V+I+GA H++
Sbjct: 248 ETTADLAGKTISVPSLFIAGTAD------PVLTFTRTDRAAEVISGPYREVLIDGAGHWL 307

Query: 308 NQESPEEVTKQIHEFI 315
            QE P EVT  + EF+
Sbjct: 308 QQERPGEVTAALLEFL 316

BLAST of MC09g0593 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.6e-42
Identity = 93/314 (29.62%), Postives = 170/314 (54.14%), Query Frame = 0

Query: 8   VISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLRGYGDTD 67
           V+   G+ +H   +G+G  ++   HGFPE W+SWR+ + + +  G+R +A D++GYG++ 
Sbjct: 242 VLIKPGVRLHFVEMGSG-PAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESS 301

Query: 68  APPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIKALVALSV 127
           APP  E Y+L  +  D++  L+   + +   +GHDWG ++ W  ALF P+R++A+ +L+ 
Sbjct: 302 APPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNT 361

Query: 128 PFPPRNPERNSVEILR--DLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSIRDPA- 187
           PF P NP  + +EI++   +F    Y   FQEPG  E + +  + +  FK F    D   
Sbjct: 362 PFMPSNPNVSPMEIIKANPVFD---YQLYFQEPGVAEAELE-QNLDRTFKNFFRAHDETF 421

Query: 188 -PPIIPKEVG--MRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWELT 247
                 +E+G    G P   SL   +++E + +Y  +F K GF G  N++R +  NW+  
Sbjct: 422 LTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWG 481

Query: 248 APWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQE 307
              SG K+ +P+  +T + D+  + P + +++     EN +  L+    I+   H+   +
Sbjct: 482 CKGSGRKILIPALMVTAENDLVLH-PKMSKHM-----ENWIPHLKR-GHIKDCGHWTQID 541

Query: 308 SPEEVTKQIHEFIQ 316
            P E+ + + E+++
Sbjct: 542 KPAELNRILIEWLE 543

BLAST of MC09g0593 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-40
Identity = 92/318 (28.93%), Postives = 162/318 (50.94%), Query Frame = 0

Query: 3   NIEHTVISTN-GIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLR 62
           ++ H  ++   GI +H   +G+G  +I   HGFPE W+SWR+ + + +  G+R +A D++
Sbjct: 234 DVSHGYVTVKPGIRLHFVEMGSG-PAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMK 293

Query: 63  GYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIKA 122
           GYGD+ +PP  E Y +  +  +++  L+   I +   +GHDW  ++ W  ALF P+R++A
Sbjct: 294 GYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRA 353

Query: 123 LVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSIR 182
           + +L+ P  P NPE + +E++R +    Y +  FQEPG  E + +   +      F +  
Sbjct: 354 VASLNTPLMPPNPEVSPMEVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRTSD 413

Query: 183 DPAPPIIPKEVGMRGY----PIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLN 242
           D     + K   M G     P    +    ++E + YY  +F K GF G  N++R    N
Sbjct: 414 DMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERN 473

Query: 243 WELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHF 302
           W+ +    G K+ VP+  +T + DI    P + +       EN +  L+    IE   H+
Sbjct: 474 WKWSCKALGRKILVPALMVTAEKDIVLR-PEMSK-----NMENWIPFLKR-GHIEDCGHW 533

Query: 303 VNQESPEEVTKQIHEFIQ 316
              E P EV + + ++++
Sbjct: 534 TQIEKPAEVNQILIKWLK 542

BLAST of MC09g0593 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.4e-40
Identity = 87/301 (28.90%), Postives = 153/301 (50.83%), Query Frame = 0

Query: 14  IDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLRGYGDTDAPPSPE 73
           + +H   +G+G  ++   HGFPE WYSWR+ + + +  GYR +A D++GYG++ APP  E
Sbjct: 248 VRLHFVELGSG-PAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIE 307

Query: 74  SYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIKALVALSVPFPPRN 133
            Y +  +  +++  LD   + +   +GHDWG ++ W  ALF P+R++A+ +L+ PF P N
Sbjct: 308 EYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPAN 367

Query: 134 PERNSVEILR--DLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSIRDPAPPIIPKE 193
           P  + +E ++   +F    Y   FQEPG  E + +  +    FK      D +   + K 
Sbjct: 368 PNMSPLESIKANPVFD---YQLYFQEPGVAEAELE-QNLSRTFKSLFRASDESVLSMHKV 427

Query: 194 VGMRGY----PIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWELTAPWSGS 253
               G     P   SL   +++E + +Y  +F K GF G  N++R +  NW+      G 
Sbjct: 428 CEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGR 487

Query: 254 KVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQESPEEVT 309
           K+ +P+  +T + D    +P + +++       D +   +   IE   H+   + P EV 
Sbjct: 488 KILIPALMVTAEKDFVL-VPQMSQHM------EDWIPHLKRGHIEDCGHWTQMDKPTEVN 536

BLAST of MC09g0593 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-39
Identity = 91/319 (28.53%), Postives = 165/319 (51.72%), Query Frame = 0

Query: 3   NIEHTVISTN-GIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLR 62
           ++ H  ++   GI +H   +G+G  ++   HGFPE W+SWR+ + + +  G+R +A D++
Sbjct: 234 DVSHGYVTVKPGIRLHFVEMGSG-PALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMK 293

Query: 63  GYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIKA 122
           GYGD+ +PP  E Y +  +  +++  LD   I +   +GHDW  ++ W  ALF P+R++A
Sbjct: 294 GYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRA 353

Query: 123 LVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSIR 182
           + +L+ PF P +P+ + ++++R +    Y +  FQEPG  E + +  +    FK F    
Sbjct: 354 VASLNTPFMPPDPDVSPMKVIRSIPVFNYQL-YFQEPGVAEAELEK-NMSRTFKSFFRAS 413

Query: 183 DPAPPII---PKEVG--MRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPL 242
           D    I      E+G  +   P   +L    ++E + +Y  +F K GF G  N++R    
Sbjct: 414 DETGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTER 473

Query: 243 NWELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAH 302
           NW+ +    G K+ VP+  +T + DI    P + + + K       +   +   IE   H
Sbjct: 474 NWKWSCKGLGRKILVPALMVTAEKDIVLR-PEMSKNMEKW------IPFLKRGHIEDCGH 533

Query: 303 FVNQESPEEVTKQIHEFIQ 316
           +   E P EV + + +++Q
Sbjct: 534 WTQIEKPTEVNQILIKWLQ 542

BLAST of MC09g0593 vs. NCBI nr
Match: KAB2616282.1 (bifunctional epoxide hydrolase 2-like [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 467 bits (1201), Expect = 3.94e-163
Identity = 209/317 (65.93%), Postives = 261/317 (82.33%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           MENIEHT++ TNGI+MHVASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MENIEHTMVPTNGINMHVASIGTGPP-VLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SYT  H+VGDLIGLLDH  ID+VFLV HDWGA++AW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  ++  R LFGD YY+CRFQEPGE+E+DFDS+DT  + K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKRFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP IPK+ G++ +  P +LP WL++E ++Y+A+KF K GFTGG NY+RAL L WEL
Sbjct: 181 RSPKPPCIPKDQGLKSWAAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +++VP KFI GDLDITY+IPG++ YIHKGGF+ DV  L+E+VV+E AAHF+NQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVAFLQEVVVMEDAAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+EV++ +++FI+K+
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

BLAST of MC09g0593 vs. NCBI nr
Match: XP_008355493.1 (uncharacterized protein LOC103419147 [Malus domestica] >RXI02556.1 hypothetical protein DVH24_002634 [Malus domestica])

HSP 1 Score: 466 bits (1200), Expect = 5.59e-163
Identity = 209/317 (65.93%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           MENIEHT + TNGI+MHVASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MENIEHTTVPTNGINMHVASIGTGPP-VLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SYT  H+VGDLIGLLDH  ID+VFLV HDWGA++AW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  ++  R LFGD YY+CRFQEPGE+E+DFDS+DT  + K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKCFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP IPK+ G++ +  P +LP WL++E ++Y+A+KF K GFTGG NY+RAL L WEL
Sbjct: 181 RSPKPPCIPKDQGLKSWAAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +++VP KFI GDLDITY+IPG++ YIHKGGF+ DV  L+E+VV+E AAHF+NQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVPFLQEVVVMEDAAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+EV++ +++FI+K+
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

BLAST of MC09g0593 vs. NCBI nr
Match: XP_009335707.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Pyrus x bretschneideri] >XP_009335715.1 PREDICTED: bifunctional epoxide hydrolase 2-like [Pyrus x bretschneideri])

HSP 1 Score: 466 bits (1200), Expect = 5.59e-163
Identity = 209/317 (65.93%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           MENIEHT + TNGI+MHVASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MENIEHTTVPTNGINMHVASIGTGPP-VLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SYT  H+VGDLIGLLDH  ID+VFLV HDWGA++AW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  ++  R LFGD YY+CRFQEPGE+E+DFDS+DT  + K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKRFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP IPK+ G++ +  P +LP WL++E ++Y+A+KF K GFTGG NY+RAL L WEL
Sbjct: 181 RSPKPPCIPKDQGLKSWAAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +++VP KFI GDLDITY+IPG++ YIHKGGF+ DV  L+E+VV+E AAHF+NQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVAFLQEVVVMEDAAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+EV++ +++FI+K+
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

BLAST of MC09g0593 vs. NCBI nr
Match: XP_007050977.1 (PREDICTED: bifunctional epoxide hydrolase 2 [Theobroma cacao] >EOX95134.1 Alpha/beta-Hydrolases superfamily protein [Theobroma cacao])

HSP 1 Score: 464 bits (1195), Expect = 3.23e-162
Identity = 212/317 (66.88%), Postives = 258/317 (81.39%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           ME IEHT+++TNGI+MHVAS+G+G   ILFLHGFPELWYSWRH LLS S LGYR +APDL
Sbjct: 1   MEKIEHTMVTTNGINMHVASVGSG-PVILFLHGFPELWYSWRHQLLSLSSLGYRCVAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPS  SYT+FH+VGDL+GLLD   +D VFLVGHDWGA+IAW F  FRPDR+K
Sbjct: 61  RGFGDTDAPPSAASYTVFHIVGDLVGLLDALGVDSVFLVGHDWGAVIAWYFCHFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV + PR+     VE  R LFGD YY+CRFQEPGE+EEDF  MDT  I KIFL+ 
Sbjct: 121 ALVNMSVAYRPRHRSMKPVEGFRALFGDDYYICRFQEPGEIEEDFAQMDTAKIMKIFLTS 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R+P PP IPKE+G RGYP P  LP WLS+E ++Y+A+KF +KGFTGG NY+RAL L+WEL
Sbjct: 181 RNPRPPCIPKEIGFRGYPDPPPLPSWLSEEDINYFASKFNEKGFTGGLNYYRALDLSWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
            APW+G +++VP KFI GDLDITY+IPG KEYIH GGF+ DV  L+E+VV+EG AHF+NQ
Sbjct: 241 MAPWTGLQIKVPVKFIVGDLDITYHIPGAKEYIHNGGFKKDVPFLQEVVVMEGVAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E  EE++K I++FI+K+
Sbjct: 301 EKSEEISKHIYDFIKKF 316

BLAST of MC09g0593 vs. NCBI nr
Match: CAZ86693.1 (epoxide hydrolase 2 [Prunus persica])

HSP 1 Score: 464 bits (1195), Expect = 3.23e-162
Identity = 212/317 (66.88%), Postives = 257/317 (81.07%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           ME IEHT +STNGI+MH+ASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MEKIEHTTVSTNGINMHIASIGTG-PVVLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SY+  H+VGDLIGLLDH  ID+VFLVGHDWGA+IAW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPASYSALHIVGDLIGLLDHLGIDQVFLVGHDWGAVIAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  V+  R LFGD YY+CRFQEPGE+E++F   DT  I K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPVDGFRALFGDDYYICRFQEPGEIEKEFAGYDTTSIMKKFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP +PKE+G+R +  P++LP WLS+E L+Y+A+KF K GF GG NY+RAL L WEL
Sbjct: 181 RSPKPPCLPKELGLRAWKTPETLPPWLSEEDLNYFASKFSKTGFVGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +V+VP KFI GDLDITY+IPGVK YIH GGF+ DV  L+E+VVIE  AHF+NQ
Sbjct: 241 TGPWTGLQVKVPVKFIVGDLDITYHIPGVKNYIHNGGFKRDVPFLQEVVVIEDGAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+E+++ +++FIQK+
Sbjct: 301 ERPDEISRHVYDFIQKF 316

BLAST of MC09g0593 vs. ExPASy TrEMBL
Match: A0A5N5GRJ8 (Bifunctional epoxide hydrolase 2-like OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_022870 PE=4 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.91e-163
Identity = 209/317 (65.93%), Postives = 261/317 (82.33%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           MENIEHT++ TNGI+MHVASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MENIEHTMVPTNGINMHVASIGTGPP-VLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SYT  H+VGDLIGLLDH  ID+VFLV HDWGA++AW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  ++  R LFGD YY+CRFQEPGE+E+DFDS+DT  + K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKRFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP IPK+ G++ +  P +LP WL++E ++Y+A+KF K GFTGG NY+RAL L WEL
Sbjct: 181 RSPKPPCIPKDQGLKSWAAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +++VP KFI GDLDITY+IPG++ YIHKGGF+ DV  L+E+VV+E AAHF+NQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVAFLQEVVVMEDAAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+EV++ +++FI+K+
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

BLAST of MC09g0593 vs. ExPASy TrEMBL
Match: A0A498K593 (AB hydrolase-1 domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_002634 PE=4 SV=1)

HSP 1 Score: 466 bits (1200), Expect = 2.71e-163
Identity = 209/317 (65.93%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           MENIEHT + TNGI+MHVASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MENIEHTTVPTNGINMHVASIGTGPP-VLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SYT  H+VGDLIGLLDH  ID+VFLV HDWGA++AW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  ++  R LFGD YY+CRFQEPGE+E+DFDS+DT  + K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKCFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP IPK+ G++ +  P +LP WL++E ++Y+A+KF K GFTGG NY+RAL L WEL
Sbjct: 181 RSPKPPCIPKDQGLKSWAAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +++VP KFI GDLDITY+IPG++ YIHKGGF+ DV  L+E+VV+E AAHF+NQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVPFLQEVVVMEDAAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+EV++ +++FI+K+
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

BLAST of MC09g0593 vs. ExPASy TrEMBL
Match: D8L7V8 (Epoxide hydrolase 2 OS=Prunus persica OX=3760 GN=eh2 PE=2 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 1.56e-162
Identity = 212/317 (66.88%), Postives = 257/317 (81.07%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           ME IEHT +STNGI+MH+ASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MEKIEHTTVSTNGINMHIASIGTG-PVVLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SY+  H+VGDLIGLLDH  ID+VFLVGHDWGA+IAW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPASYSALHIVGDLIGLLDHLGIDQVFLVGHDWGAVIAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  V+  R LFGD YY+CRFQEPGE+E++F   DT  I K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPVDGFRALFGDDYYICRFQEPGEIEKEFAGYDTTSIMKKFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP +PKE+G+R +  P++LP WLS+E L+Y+A+KF K GF GG NY+RAL L WEL
Sbjct: 181 RSPKPPCLPKELGLRAWKTPETLPPWLSEEDLNYFASKFSKTGFVGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +V+VP KFI GDLDITY+IPGVK YIH GGF+ DV  L+E+VVIE  AHF+NQ
Sbjct: 241 TGPWTGLQVKVPVKFIVGDLDITYHIPGVKNYIHNGGFKRDVPFLQEVVVIEDGAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+E+++ +++FIQK+
Sbjct: 301 ERPDEISRHVYDFIQKF 316

BLAST of MC09g0593 vs. ExPASy TrEMBL
Match: A0A061DQR1 (Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_004689 PE=4 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 1.56e-162
Identity = 212/317 (66.88%), Postives = 258/317 (81.39%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           ME IEHT+++TNGI+MHVAS+G+G   ILFLHGFPELWYSWRH LLS S LGYR +APDL
Sbjct: 1   MEKIEHTMVTTNGINMHVASVGSG-PVILFLHGFPELWYSWRHQLLSLSSLGYRCVAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPS  SYT+FH+VGDL+GLLD   +D VFLVGHDWGA+IAW F  FRPDR+K
Sbjct: 61  RGFGDTDAPPSAASYTVFHIVGDLVGLLDALGVDSVFLVGHDWGAVIAWYFCHFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV + PR+     VE  R LFGD YY+CRFQEPGE+EEDF  MDT  I KIFL+ 
Sbjct: 121 ALVNMSVAYRPRHRSMKPVEGFRALFGDDYYICRFQEPGEIEEDFAQMDTAKIMKIFLTS 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R+P PP IPKE+G RGYP P  LP WLS+E ++Y+A+KF +KGFTGG NY+RAL L+WEL
Sbjct: 181 RNPRPPCIPKEIGFRGYPDPPPLPSWLSEEDINYFASKFNEKGFTGGLNYYRALDLSWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
            APW+G +++VP KFI GDLDITY+IPG KEYIH GGF+ DV  L+E+VV+EG AHF+NQ
Sbjct: 241 MAPWTGLQIKVPVKFIVGDLDITYHIPGAKEYIHNGGFKKDVPFLQEVVVMEGVAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E  EE++K I++FI+K+
Sbjct: 301 EKSEEISKHIYDFIKKF 316

BLAST of MC09g0593 vs. ExPASy TrEMBL
Match: A0A540MNH6 (AB hydrolase-1 domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_014066 PE=4 SV=1)

HSP 1 Score: 464 bits (1194), Expect = 2.22e-162
Identity = 208/317 (65.62%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           M+NIEHT + TNGI+MHVASIGTG   +LFLHGFPELWYSWRH LLS S LGYR IAPDL
Sbjct: 1   MDNIEHTTVPTNGINMHVASIGTGPP-VLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RG+GDTDAPPSP SYT  H+VGDLIGLLDH  ID+VFLV HDWGA++AW F LFRPDR+K
Sbjct: 61  RGFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVK 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV +SV F PRNP+R  ++  R LFGD YY+CRFQEPGE+E+DFDS+DT  + K FL+ 
Sbjct: 121 ALVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKCFLTG 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R P PP IPK+ G++ +  P +LP WL++E ++Y+A+KF K GFTGG NY+RAL L WEL
Sbjct: 181 RSPKPPCIPKDQGLKFWTAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           T PW+G +++VP KFI GDLDITY+IPG++ YIHKGGF+ DV  L+E+VV+E AAHF+NQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVPFLQEVVVMEDAAHFINQ 300

Query: 301 ESPEEVTKQIHEFIQKY 317
           E P+EV++ +++FI+K+
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

BLAST of MC09g0593 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 450.3 bits (1157), Expect = 1.3e-126
Identity = 203/317 (64.04%), Postives = 249/317 (78.55%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDSILFLHGFPELWYSWRHHLLSFSDLGYRAIAPDL 60
           ME IEHT ISTNGI+MHVASIG+G   ILF+HGFP+LWYSWRH L+SF+ LGYRAIAPDL
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSG-PVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDL 60

Query: 61  RGYGDTDAPPSPESYTLFHVVGDLIGLLDHFDIDRVFLVGHDWGALIAWAFALFRPDRIK 120
           RGYGD+DAPPS ESYT+ H+VGDL+GLLD   +DRVFLVGHDWGA++AW   + RPDR+ 
Sbjct: 61  RGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVN 120

Query: 121 ALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFLSI 180
           ALV  SV F PRNP    V+  R LFGD YY+CRFQEPGE+EEDF  +DT+ +   F + 
Sbjct: 121 ALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTS 180

Query: 181 RDPAPPIIPKEVGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALPLNWEL 240
           R+P PP IPK VG RG P P SLP WL+++ + +Y  KF +KGFTGG NY+RAL L+WEL
Sbjct: 181 RNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWEL 240

Query: 241 TAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAAHFVNQ 300
           TAPW+G +++VP KFI GDLDITYNIPG KEYIH+GG +  V  L+E+VV+EG  HF++Q
Sbjct: 241 TAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQ 300

Query: 301 ESPEEVTKQIHEFIQKY 318
           E P+EVT  I+ F +K+
Sbjct: 301 EKPDEVTDHIYGFFKKF 316

BLAST of MC09g0593 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.0 bits (946), Expect = 3.8e-102
Identity = 173/323 (53.56%), Postives = 223/323 (69.04%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVASIGTGSDS-ILFLHGFPELWYSWRHHLLSFSDLGYRAIAPD 60
           ME I+H ++S NGI MH+A  G      +L LHGFP+LWY+WRH +   S LGYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDTDAPPSPESYTLFHVVGDLIGLLDHF--DIDRVFLVGHDWGALIAWAFALFRPD 120
           LRGYGD+D+P S   YT  +VVGDL+ LLD    + ++VFLVGHDWGA+I W   LFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIF 180
           +I   V LSVP+  RNP+   V+  + +FGD YY+CRFQEPG++E +  S D     +  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LSIRDPAPPIIPKEVGMRGYPIPDS----LPCWLSQESLSYYATKFGKKGFTGGFNYFRA 240
            + R   PPI+PK+      P P+S    LP W S++ L +Y +KF K GFTGG NY+RA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 LPLNWELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEG 300
           + LNWELTAPW+G+K++VP KF+TGD D+ Y  PG+KEYIH GGF  DV  L+EIVVIE 
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 AAHFVNQESPEEVTKQIHEFIQK 317
           A HFVNQE P+EVT  I++F  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of MC09g0593 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 2.7e-100
Identity = 169/324 (52.16%), Postives = 224/324 (69.14%), Query Frame = 0

Query: 1   MENIEHTVISTNGIDMHVA---SIGTGSDS-ILFLHGFPELWYSWRHHLLSFSDLGYRAI 60
           ++ +EH  +  NGI+MHVA     G+G D  ILFLHGFPELWY+WRH +++ S LGYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDTDAPPSPESYTLFHVVGDLIGLLDHF--DIDRVFLVGHDWGALIAWAFALF 120
           APDLRGYGDT+AP   E YT  +V GD++ L+D        V +VGHDWGA+IAW    +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRIKALVALSVPFPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIF 180
           RP+++KALV +SV F PRNP R  V  LR +FGD YYVCRFQ+ GE+E +F  + TE++ 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KIFLSIRDPAPPIIPKE-VGMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRA 240
           K FL+ + P P  +PK+    R      +LP WL+QE L YY TK+  KGFTG  NY+R 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 LPLNWELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEG 300
           +  NWELTAPW+G+K+ VP KFI GD D+TYN PG KEYI+ GGF+ DV  L+E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFVNQESPEEVTKQIHEFIQKY 318
             HF+++E+P+ + + IH F  K+
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of MC09g0593 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 345.1 bits (884), Expect = 5.9e-95
Identity = 169/323 (52.32%), Postives = 222/323 (68.73%), Query Frame = 0

Query: 4   IEHTVISTNGIDMHVASIGTGSDSI-LFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLRG 63
           +EH  +  NGID+HVA  G     I L LHGFPELWYSWRH +   +  GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDTDAPPSPESYTLFHVVGDLIGLLDHF---DIDRVFLVGHDWGALIAWAFALFRPDRI 123
           YGD+DAP    SYT F++VGDLI ++      + ++VF+VGHDWGALIAW   LFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVALSVPFP--PRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIF 183
           KALV LSVPF   P +P    V+ +R  +GD YY+CRFQE G++E +   + TE + K  
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LSIRDPAPPIIPKEV---GMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRAL 243
           L+ R P P IIPK+    G +G  IP  LP WL++E ++Y+ +KF +KGF+G  NY+R  
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNF 240

Query: 244 PLNWELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGA 303
             N EL  PW GSK++VP+KF+ G+LD+ Y +PGVKEYIH   F+ DV  LEE VV+EG 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 304 AHFVNQESPEEVTKQIHEFIQKY 318
           AHF+NQE P+E+ + I +FI K+
Sbjct: 301 AHFINQEKPQEILQIILDFISKF 321

BLAST of MC09g0593 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 4.7e-92
Identity = 164/322 (50.93%), Postives = 221/322 (68.63%), Query Frame = 0

Query: 4   IEHTVISTNGIDMHVASIGTGSDSI-LFLHGFPELWYSWRHHLLSFSDLGYRAIAPDLRG 63
           +EH  +  NGID+HVA  G    +I L LHGFPELWYSWRH +   +  GYRA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDTDAPPSPESYTLFHVVGDLIGLLDHF--DIDRVFLVGHDWGALIAWAFALFRPDRIK 123
           YGD+DAP    S+T F++VGDL+ ++     +  +VF+VGHDWGALIAW   LFRPD++K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVALSVP--FPPRNPERNSVEILRDLFGDGYYVCRFQEPGEMEEDFDSMDTEDIFKIFL 183
           ALV LSVP  F P +P    V+ +R ++G+ YYVCRFQE G++E +   + TE + K  L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 SIRDPAPPIIPKEV---GMRGYPIPDSLPCWLSQESLSYYATKFGKKGFTGGFNYFRALP 243
           + R P P IIPK+    G +G  IP  LP WL++E ++Y+ +KF +KGF G  NY+R   
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFN 240

Query: 244 LNWELTAPWSGSKVEVPSKFITGDLDITYNIPGVKEYIHKGGFENDVVGLEEIVVIEGAA 303
            N EL  PW GSK++VP+KF+ G+LD+ Y +PGVKEYIH   F+ DV  +EE VV+EG A
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 304 HFVNQESPEEVTKQIHEFIQKY 318
           HF+NQE P+E+ + I +FI  +
Sbjct: 301 HFLNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS09.9e-5536.39Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C25.6e-4229.62Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802991.8e-4028.93Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349132.4e-4028.90Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349141.2e-3928.53Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
KAB2616282.13.94e-16365.93bifunctional epoxide hydrolase 2-like [Pyrus ussuriensis x Pyrus communis][more]
XP_008355493.15.59e-16365.93uncharacterized protein LOC103419147 [Malus domestica] >RXI02556.1 hypothetical ... [more]
XP_009335707.15.59e-16365.93PREDICTED: bifunctional epoxide hydrolase 2-like [Pyrus x bretschneideri] >XP_00... [more]
XP_007050977.13.23e-16266.88PREDICTED: bifunctional epoxide hydrolase 2 [Theobroma cacao] >EOX95134.1 Alpha/... [more]
CAZ86693.13.23e-16266.88epoxide hydrolase 2 [Prunus persica][more]
Match NameE-valueIdentityDescription
A0A5N5GRJ81.91e-16365.93Bifunctional epoxide hydrolase 2-like OS=Pyrus ussuriensis x Pyrus communis OX=2... [more]
A0A498K5932.71e-16365.93AB hydrolase-1 domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_002... [more]
D8L7V81.56e-16266.88Epoxide hydrolase 2 OS=Prunus persica OX=3760 GN=eh2 PE=2 SV=1[more]
A0A061DQR11.56e-16266.88Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_0046... [more]
A0A540MNH62.22e-16265.62AB hydrolase-1 domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_014... [more]
Match NameE-valueIdentityDescription
AT4G02340.11.3e-12664.04alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.13.8e-10253.56alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.12.7e-10052.16alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.15.9e-9552.32soluble epoxide hydrolase [more]
AT2G26750.14.7e-9250.93alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 112..125
score: 32.76
coord: 52..67
score: 44.61
coord: 98..111
score: 36.7
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 27..152
e-value: 3.4E-22
score: 79.3
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 112..125
score: 48.38
coord: 291..313
score: 40.51
coord: 32..50
score: 48.33
coord: 52..67
score: 60.8
coord: 98..111
score: 53.57
coord: 251..267
score: 38.77
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..317
e-value: 8.5E-100
score: 336.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..316
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..317
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0593.1MC09g0593.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity