MC09g0584 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0584
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectin lyase-like superfamily protein
Location	MC09: 5302183 .. 5303338 (+)
RNA-Seq Expression	MC09g0584
Synteny	MC09g0584

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGTCACCCATGACTGTCGAGATTCAGGGAACTGTCAAAGCTACCACAGATCTTTCTGAATATTCTGGTCCGGATTGGATTCTGTTCGAATCTGTGAACGATCTGACGATCACCGGGAGTGGAACCTTCGACGGGCAAGGTCAGGAAGTATGGAAGTTCAACGATTGTAGTCAAAATCCCCGTTGTGTACTTCTGGCGCACACAATCAAGTTAAACAAGGTGAAAAAAGGGTTGGTAGAGCACGTTTCTTCAGTGAACGCCAAAGCCTTCCATTTCTTCTTGTTTGCTTGCAATGATATTAGCTTCAACAATGTCAAAATAATAGCTCCTGGCGAGAGCCCCAACACAGATGGAATTCACACGAGCGAATCTAATCTTATCAATATAACAAACACCTACATTAGCACTGGAGATGACTGTGTTTCCATTGGCCAAGGCACCACCAATATCAACGTTCACAATGTCACCTGTGCCCCAGGACATGGAATCAGGTATTCAATCTTTTCCCATTTGAAATAGTATTATATGCAAATTTTGTGCTAACATATGCAGATTTTGCAGTGTGGGAAGTTTAGGAAAATACCAAGATGAGAAGGACGTTGTGGGAGTTACCGTCACAAATTGCACGTTGAGAAACACCACAAATGGATTAAGAATCAAGACATGGGCTGATTCACGTCCAACCCAAGCTTCCAAAATCAGTTTCCAAGACATTATCTTAGATGCAGTCAAAAACCCCATTATCATAGATCAAAGCTATGGCTCCAAAGATAAGAGCAAACCCGTAAGTATTAGAACAAGCAACAAGAACGTTACTAAACGATCCATAAAGACTTCGTTTGATAACGATCATGACCATGTTATTATATCTTACAGTGAGTTTGAAGTATTATTATTAATGTTGTTCATGTGTTTGATATGCATGCAGCCATCTCAAGTGAAGATAAGCGAGGTCAATTACAAGAACATTCGAGGCACGACGATTTCGGTCGTCCCCGTTTCGTTGCAGTGTAGTGCTAAAGTTCCGTGCGAGGGCGTCAAATTGGAAGAGATTGATATGTCCTTTGTTGGCTCAGAGAAGAAGAAGGCAGCTTTTGGAAATTCATGTTTGAATGCCAAGATTACAACCGTAGGAAAACAAATCCCACCAGCTTGT

mRNA sequence

ACGTCACCCATGACTGTCGAGATTCAGGGAACTGTCAAAGCTACCACAGATCTTTCTGAATATTCTGGTCCGGATTGGATTCTGTTCGAATCTGTGAACGATCTGACGATCACCGGGAGTGGAACCTTCGACGGGCAAGGTCAGGAAGTATGGAAGTTCAACGATTGTAGTCAAAATCCCCGTTGTGTACTTCTGGCGCACACAATCAAGTTAAACAAGGTGAAAAAAGGGTTGGTAGAGCACGTTTCTTCAGTGAACGCCAAAGCCTTCCATTTCTTCTTGTTTGCTTGCAATGATATTAGCTTCAACAATGTCAAAATAATAGCTCCTGGCGAGAGCCCCAACACAGATGGAATTCACACGAGCGAATCTAATCTTATCAATATAACAAACACCTACATTAGCACTGGAGATGACTGTGTTTCCATTGGCCAAGGCACCACCAATATCAACGTTCACAATGTCACCTGTGCCCCAGGACATGGAATCAGTGTGGGAAGTTTAGGAAAATACCAAGATGAGAAGGACGTTGTGGGAGTTACCGTCACAAATTGCACGTTGAGAAACACCACAAATGGATTAAGAATCAAGACATGGGCTGATTCACGTCCAACCCAAGCTTCCAAAATCAGTTTCCAAGACATTATCTTAGATGCAGTCAAAAACCCCATTATCATAGATCAAAGCTATGGCTCCAAAGATAAGAGCAAACCCCCATCTCAAGTGAAGATAAGCGAGGTCAATTACAAGAACATTCGAGGCACGACGATTTCGGTCGTCCCCGTTTCGTTGCAGTGTAGTGCTAAAGTTCCGTGCGAGGGCGTCAAATTGGAAGAGATTGATATGTCCTTTGTTGGCTCAGAGAAGAAGAAGGCAGCTTTTGGAAATTCATGTTTGAATGCCAAGATTACAACCGTAGGAAAACAAATCCCACCAGCTTGT

Coding sequence (CDS)

ACGTCACCCATGACTGTCGAGATTCAGGGAACTGTCAAAGCTACCACAGATCTTTCTGAATATTCTGGTCCGGATTGGATTCTGTTCGAATCTGTGAACGATCTGACGATCACCGGGAGTGGAACCTTCGACGGGCAAGGTCAGGAAGTATGGAAGTTCAACGATTGTAGTCAAAATCCCCGTTGTGTACTTCTGGCGCACACAATCAAGTTAAACAAGGTGAAAAAAGGGTTGGTAGAGCACGTTTCTTCAGTGAACGCCAAAGCCTTCCATTTCTTCTTGTTTGCTTGCAATGATATTAGCTTCAACAATGTCAAAATAATAGCTCCTGGCGAGAGCCCCAACACAGATGGAATTCACACGAGCGAATCTAATCTTATCAATATAACAAACACCTACATTAGCACTGGAGATGACTGTGTTTCCATTGGCCAAGGCACCACCAATATCAACGTTCACAATGTCACCTGTGCCCCAGGACATGGAATCAGTGTGGGAAGTTTAGGAAAATACCAAGATGAGAAGGACGTTGTGGGAGTTACCGTCACAAATTGCACGTTGAGAAACACCACAAATGGATTAAGAATCAAGACATGGGCTGATTCACGTCCAACCCAAGCTTCCAAAATCAGTTTCCAAGACATTATCTTAGATGCAGTCAAAAACCCCATTATCATAGATCAAAGCTATGGCTCCAAAGATAAGAGCAAACCCCCATCTCAAGTGAAGATAAGCGAGGTCAATTACAAGAACATTCGAGGCACGACGATTTCGGTCGTCCCCGTTTCGTTGCAGTGTAGTGCTAAAGTTCCGTGCGAGGGCGTCAAATTGGAAGAGATTGATATGTCCTTTGTTGGCTCAGAGAAGAAGAAGGCAGCTTTTGGAAATTCATGTTTGAATGCCAAGATTACAACCGTAGGAAAACAAATCCCACCAGCTTGT

Protein sequence

TSPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRCVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAKITTVGKQIPPAC

Homology

[Close]

BLAST of MC09g0584 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-82
Identity = 154/319 (48.28%), Postives = 218/319 (68.34%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           S +TV + GT+ ATT  S Y+ P+W LFE V++L +TG+GTF G+G+ VWK + C +  +
Sbjct: 107 SKVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQ 166

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
           C L   ++K   +K   +  +SSVNAKAFH FL    +++  N+K+ AP ESPNTDGIH 
Sbjct: 167 CNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHL 226

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
           S ++ ++I ++ I+TGDDCVS+G+G+ N+ V  V C PGHG+SVGSLGKY++E+DV G+ 
Sbjct: 227 SNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIH 286

Query: 182 VTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ 241
           V NCT+  T NGLRIKTW  S P++A  I F++II+ +VKNPIIIDQ+YGS+      SQ
Sbjct: 287 VNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD---SQ 346

Query: 242 VKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFV---GSEKKKAA---FG 301
           V IS++ +KNIRGTTI+   V + CS  VPC+GV + ++++ +V   G EKK ++    G
Sbjct: 347 VAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVG 406

Query: 302 NSCLNAKITTVGKQIPPAC 315
             C NA +   GK   P C
Sbjct: 407 ALCDNANVIFGGKLSFPMC 420

[Close]

BLAST of MC09g0584 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-76
Identity = 135/312 (43.27%), Postives = 202/312 (64.74%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           S +  +I G VKA  D S++    W+ F  ++ LT++G+GT DGQGQ  W  N+C +NP 
Sbjct: 64  SKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPN 123

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
           C   A  ++ + +K  +V  ++S+N+K FH  +  C DI+F +V + APG S NTDGIH 
Sbjct: 124 CKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHV 183

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
             S  + ITNT I+TGDDC+SIG G+ N+ +  V C PGHGIS+GSLG+Y +EK+V G+T
Sbjct: 184 GISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGIT 243

Query: 182 VTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSY--GSKDKSKPP 241
           V  CT   T NG+R+KTW +S P  A+ ++FQD+ ++ V+NP+I+DQ Y    +   + P
Sbjct: 244 VKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAP 303

Query: 242 SQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCL 301
           S++K+S +N+ NIRGT+   V V + CS  +PC  +K+ EI++S+ G+        ++C 
Sbjct: 304 SRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPAT---STCS 363

Query: 302 NAKITTVGKQIP 312
           N K T  GKQ+P
Sbjct: 364 NVKPTFSGKQVP 372

[Close]

BLAST of MC09g0584 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-75
Identity = 142/316 (44.94%), Postives = 206/316 (65.19%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQN-P 61
           S + +++QGT+KA  D S+  G  WI    ++ LTI G G FDGQG+  W  NDC +N P
Sbjct: 42  SSIGLQLQGTLKAPADPSKIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGP 101

Query: 62  RCVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIH 121
            C  L+  ++L  V   ++  V+++++K FH  +  C +++F   KI A   S NTDGIH
Sbjct: 102 ICKTLSMNLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIH 161

Query: 122 TSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGV 181
              S+ +NI NT I TGDDC+S+G G+ NIN+ N+TC PGHGISVGSLG+Y++E+ VVG+
Sbjct: 162 IGRSDGVNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGI 221

Query: 182 TVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDK--SKP 241
            V NCT+  + NG+RIKTW  S P +AS++ FQDI +++V  PI+IDQ Y   ++  ++ 
Sbjct: 222 YVKNCTITGSQNGVRIKTWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEV 281

Query: 242 PSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSC 301
           PS VK+S++++KNI+GT+ +   V L CS   PC GV+L +ID+++ G   K     + C
Sbjct: 282 PSSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSG---KGGPATSVC 341

Query: 302 LNAKITTVGKQIPPAC 315
            N K T  GKQIP  C
Sbjct: 342 ENIKPTIKGKQIPAIC 354

[Close]

BLAST of MC09g0584 vs. ExPASy Swiss-Prot
Match: W8P8Q3 (Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 PE=1 SV=3)

HSP 1 Score: 272.3 bits (695), Expect = 6.7e-72
Identity = 135/319 (42.32%), Postives = 197/319 (61.76%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPD-WILFESVNDLTIT---GSGTFDGQGQEVWKFNDCS 61
           +P+T+EI G  KA  D+++  G D W+  E+V  LTIT     GTFDGQGQ  WK N C+
Sbjct: 79  APLTIEIAGNFKAPADVAQMKGEDTWVKIENVQGLTITCLPTGGTFDGQGQAAWKQNKCA 138

Query: 62  QNPRCVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTD 121
           Q+  C  L +  + N +    +  + S+N+K +H  +  C +I+   + I AP +S NTD
Sbjct: 139 QSGMCNSLPYNFRFNTLTNAQISGIKSLNSKLYHMGVMGCKNITLTGLTIDAPKDSLNTD 198

Query: 122 GIHTSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDV 181
           G+H   SN ++ TN+ I TGDDC+S+G G  +++V  +TC PGHGIS+GS+GK+ +E   
Sbjct: 199 GMHIGRSNGVHATNSKIGTGDDCISMGDGAVDVHVEGITCGPGHGISIGSMGKFANEAPN 258

Query: 182 VGVTVTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKD--K 241
            G+ V NC+  +T NG+RIK+W +S    AS + F+DI +  V NP+IIDQ Y   +  K
Sbjct: 259 TGIFVKNCSFTDTDNGVRIKSWMNSFEASASDLHFEDITVTNVLNPVIIDQEYCPYNHCK 318

Query: 242 SKPPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFG 301
            K PS+VK+S++++KN+ G   S   V L CS+ VPC+GV+L +ID++F G      A  
Sbjct: 319 EKTPSKVKLSKISFKNVHGAAKSAEVVKLLCSSAVPCDGVELADIDLTFPG-----GAAV 378

Query: 302 NSCLNAKITTVGKQIPPAC 315
           + C N K    GKQ P AC
Sbjct: 379 SQCKNVKPIVTGKQNPVAC 392

[Close]

BLAST of MC09g0584 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.3e-71
Identity = 133/315 (42.22%), Postives = 195/315 (61.90%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           +P+ + +QGT++A  D S +  P+W+ F SV +  + G G FDGQG   ++ N C     
Sbjct: 85  APIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREF 144

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
              L   I+ + V   L++ ++S ++K FH  +FAC +I+   +KI AP ESPNTDGIH 
Sbjct: 145 RSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHM 204

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
            +S  +NI  + I TGDDC+SIG GT N+ +  +TC PGHGIS+GSLGK+Q+E+ V G+ 
Sbjct: 205 GKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIK 264

Query: 182 VTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSY--GSKDKSKPP 241
           ++NCT+ NT+NG RIKTW        S+I F+DI ++ V +PI+IDQ Y   +K K    
Sbjct: 265 ISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEE 324

Query: 242 SQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCL 301
           S+VK+S +++KNIRGT+     +   CS   PC+ V+L +ID+   G+E       + CL
Sbjct: 325 SKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAEPAT----SQCL 384

Query: 302 NAKITTVGKQIPPAC 315
           N K  T+GK  P  C
Sbjct: 385 NVKPITIGKLNPIPC 395

[Close]

BLAST of MC09g0584 vs. NCBI nr
Match: XP_022146404.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 593 bits (1528), Expect = 4.94e-211
Identity = 285/313 (91.05%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           SPMTVEIQGTVK +TD+S+YSGPDWILFES+NDLTITGSGTFDGQGQEVWKFNDCSQNP 
Sbjct: 125 SPMTVEIQGTVKGSTDVSQYSGPDWILFESINDLTITGSGTFDGQGQEVWKFNDCSQNPN 184

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
           CVLLAH+IKLNKVKKGLVEH+SSVNAKAFH FLFACNDI FNN+KIIAPGESPNTDGIHT
Sbjct: 185 CVLLAHSIKLNKVKKGLVEHISSVNAKAFHIFLFACNDIRFNNLKIIAPGESPNTDGIHT 244

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
           SESNLINITNT+ISTGDDCVSIGQGTTNI+VHNVTCAPGHG+SVGSLGKY+DEKDV+GVT
Sbjct: 245 SESNLINITNTFISTGDDCVSIGQGTTNIHVHNVTCAPGHGLSVGSLGKYKDEKDVMGVT 304

Query: 182 VTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ 241
           +TNCTLRNTTNGLRIKTWADS PTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ
Sbjct: 305 ITNCTLRNTTNGLRIKTWADSPPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ 364

Query: 242 VKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNA 301
           VKISEVNYKN+RGTTISVVPVSLQCS KVPCEG+KLEEID+SFVGS+KK  AF N+C+NA
Sbjct: 365 VKISEVNYKNVRGTTISVVPVSLQCSPKVPCEGIKLEEIDLSFVGSDKKMTAFANACVNA 424

Query: 302 KITTVGKQIPPAC 314
           KITTVGKQIPPAC
Sbjct: 425 KITTVGKQIPPAC 437

[Close]

BLAST of MC09g0584 vs. NCBI nr
Match: KAA0046656.1 (exopolygalacturonase clone [Cucumis melo var. makuwa])

HSP 1 Score: 442 bits (1136), Expect = 1.41e-151
Identity = 210/312 (67.31%), Postives = 260/312 (83.33%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD++EYS P+WILFESV  L + G GTFDGQG E WK+NDCS++P+C
Sbjct: 119 PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQC 178

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
            L   +IK NKV  G +E  +SVN+KAFH F+   ++I  NN+ I+APG SPNTDG+H S
Sbjct: 179 TLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHIS 238

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 239 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFV 298

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK K + PSQV
Sbjct: 299 SNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK-KKRSPSQV 358

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           KIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS  K+  FGNSCLNAK
Sbjct: 359 KISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQP-FGNSCLNAK 418

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 419 ITTIGKQNPPAC 428

[Close]

BLAST of MC09g0584 vs. NCBI nr
Match: XP_008451547.1 (PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo] >TYK18191.1 exopolygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 442 bits (1136), Expect = 1.41e-151
Identity = 210/312 (67.31%), Postives = 260/312 (83.33%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD++EYS P+WILFESV  L + G GTFDGQG E WK+NDCS++P+C
Sbjct: 119 PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQC 178

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
            L   +IK NKV  G +E  +SVN+KAFH F+   ++I  NN+ I+APG SPNTDG+H S
Sbjct: 179 TLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHIS 238

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 239 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFV 298

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK K + PSQV
Sbjct: 299 SNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK-KKRSPSQV 358

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           KIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS  K+  FGNSCLNAK
Sbjct: 359 KISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQP-FGNSCLNAK 418

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 419 ITTIGKQNPPAC 428

[Close]

BLAST of MC09g0584 vs. NCBI nr
Match: XP_011659369.1 (exopolygalacturonase clone GBGE184 [Cucumis sativus] >KGN44972.1 hypothetical protein Csa_015742 [Cucumis sativus])

HSP 1 Score: 439 bits (1129), Expect = 1.86e-150
Identity = 207/312 (66.35%), Postives = 263/312 (84.29%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD+S+YS  +WILFESV  L + G GTFDGQG + WK+NDCS++P+C
Sbjct: 123 PMTVEIQGTVKATTDISQYSSAEWILFESVTGLNLIGRGTFDGQGADTWKYNDCSKHPQC 182

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
           ++   +IK NKV +GL+E ++SVN+KAFH F+   ++I  NNV I+APG SPNTDG+H S
Sbjct: 183 IIPPTSIKFNKVTQGLMEGITSVNSKAFHIFVVLSHNIKINNVHIVAPGNSPNTDGVHIS 242

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 243 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYRDEKEVRGIFV 302

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQ+I+LD V+NPIIIDQ+YGSK K K PSQV
Sbjct: 303 SNCTIRNTTNGVRIKTWAASPPGQATRITFQNIVLDKVRNPIIIDQNYGSKTK-KSPSQV 362

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           K+S+V +KNIRGTTIS V VSLQCSA +PC+G+KLE+ID++F G+  K+  F NSCLNAK
Sbjct: 363 KVSDVQFKNIRGTTISPVAVSLQCSAALPCDGIKLEQIDVAFSGTHLKQP-FANSCLNAK 422

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 423 ITTIGKQNPPAC 432

[Close]

BLAST of MC09g0584 vs. NCBI nr
Match: XP_038899827.1 (exopolygalacturonase clone GBGE184 [Benincasa hispida])

HSP 1 Score: 438 bits (1127), Expect = 3.62e-150
Identity = 212/312 (67.95%), Postives = 256/312 (82.05%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD++EYS  +WILFES+  L++ GSGTFDGQG E W +NDC ++ RC
Sbjct: 121 PMTVEIQGTVKATTDITEYSSAEWILFESITGLSLIGSGTFDGQGAETWMYNDCRKHSRC 180

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
           VL   +IK NKV  GL+E +SS+N+KAFH F+   ++I  NN+ I APG SPNTDGIH S
Sbjct: 181 VLPPTSIKFNKVTNGLMEGISSLNSKAFHIFVVLSHNIKVNNLHITAPGNSPNTDGIHIS 240

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++NITN+ I TGDDCVSIG G+TNI V NVTC PGHGISVGSLGKY DEK+V G+ V
Sbjct: 241 QTDVVNITNSIIGTGDDCVSIGHGSTNITVSNVTCGPGHGISVGSLGKYDDEKEVRGIFV 300

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNGLRIKTWA S   QA++I+FQDIILD+V+NPIIIDQ+YGSK K K PSQV
Sbjct: 301 SNCTIRNTTNGLRIKTWAASPAGQATRITFQDIILDSVRNPIIIDQTYGSK-KKKAPSQV 360

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           KIS+V +KNIRGTT+S V VSLQCSA VPC+G+KLEEID++F G+  K   F NSCLNAK
Sbjct: 361 KISDVVFKNIRGTTVSDVGVSLQCSAAVPCDGIKLEEIDLAFTGTRLKHPTFANSCLNAK 420

Query: 303 ITTVGKQIPPAC 314
           IT +GKQ PPAC
Sbjct: 421 ITAIGKQNPPAC 431

[Close]

BLAST of MC09g0584 vs. ExPASy TrEMBL
Match: A0A6J1CY21 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111015625 PE=3 SV=1)

HSP 1 Score: 593 bits (1528), Expect = 2.39e-211
Identity = 285/313 (91.05%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           SPMTVEIQGTVK +TD+S+YSGPDWILFES+NDLTITGSGTFDGQGQEVWKFNDCSQNP 
Sbjct: 125 SPMTVEIQGTVKGSTDVSQYSGPDWILFESINDLTITGSGTFDGQGQEVWKFNDCSQNPN 184

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
           CVLLAH+IKLNKVKKGLVEH+SSVNAKAFH FLFACNDI FNN+KIIAPGESPNTDGIHT
Sbjct: 185 CVLLAHSIKLNKVKKGLVEHISSVNAKAFHIFLFACNDIRFNNLKIIAPGESPNTDGIHT 244

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
           SESNLINITNT+ISTGDDCVSIGQGTTNI+VHNVTCAPGHG+SVGSLGKY+DEKDV+GVT
Sbjct: 245 SESNLINITNTFISTGDDCVSIGQGTTNIHVHNVTCAPGHGLSVGSLGKYKDEKDVMGVT 304

Query: 182 VTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ 241
           +TNCTLRNTTNGLRIKTWADS PTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ
Sbjct: 305 ITNCTLRNTTNGLRIKTWADSPPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ 364

Query: 242 VKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNA 301
           VKISEVNYKN+RGTTISVVPVSLQCS KVPCEG+KLEEID+SFVGS+KK  AF N+C+NA
Sbjct: 365 VKISEVNYKNVRGTTISVVPVSLQCSPKVPCEGIKLEEIDLSFVGSDKKMTAFANACVNA 424

Query: 302 KITTVGKQIPPAC 314
           KITTVGKQIPPAC
Sbjct: 425 KITTVGKQIPPAC 437

[Close]

BLAST of MC09g0584 vs. ExPASy TrEMBL
Match: A0A5D3D1R6 (Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001270 PE=3 SV=1)

HSP 1 Score: 442 bits (1136), Expect = 6.81e-152
Identity = 210/312 (67.31%), Postives = 260/312 (83.33%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD++EYS P+WILFESV  L + G GTFDGQG E WK+NDCS++P+C
Sbjct: 119 PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQC 178

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
            L   +IK NKV  G +E  +SVN+KAFH F+   ++I  NN+ I+APG SPNTDG+H S
Sbjct: 179 TLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHIS 238

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 239 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFV 298

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK K + PSQV
Sbjct: 299 SNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK-KKRSPSQV 358

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           KIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS  K+  FGNSCLNAK
Sbjct: 359 KISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQP-FGNSCLNAK 418

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 419 ITTIGKQNPPAC 428

[Close]

BLAST of MC09g0584 vs. ExPASy TrEMBL
Match: A0A5A7TXI9 (Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold427G00200 PE=3 SV=1)

HSP 1 Score: 442 bits (1136), Expect = 6.81e-152
Identity = 210/312 (67.31%), Postives = 260/312 (83.33%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD++EYS P+WILFESV  L + G GTFDGQG E WK+NDCS++P+C
Sbjct: 119 PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQC 178

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
            L   +IK NKV  G +E  +SVN+KAFH F+   ++I  NN+ I+APG SPNTDG+H S
Sbjct: 179 TLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHIS 238

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 239 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFV 298

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK K + PSQV
Sbjct: 299 SNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK-KKRSPSQV 358

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           KIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS  K+  FGNSCLNAK
Sbjct: 359 KISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQP-FGNSCLNAK 418

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 419 ITTIGKQNPPAC 428

[Close]

BLAST of MC09g0584 vs. ExPASy TrEMBL
Match: A0A1S3BRS3 (exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 SV=1)

HSP 1 Score: 442 bits (1136), Expect = 6.81e-152
Identity = 210/312 (67.31%), Postives = 260/312 (83.33%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD++EYS P+WILFESV  L + G GTFDGQG E WK+NDCS++P+C
Sbjct: 119 PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQC 178

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
            L   +IK NKV  G +E  +SVN+KAFH F+   ++I  NN+ I+APG SPNTDG+H S
Sbjct: 179 TLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHIS 238

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 239 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFV 298

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQDI+LD V+NPIIIDQ+YGSK K + PSQV
Sbjct: 299 SNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSK-KKRSPSQV 358

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           KIS+V +KNIRGTTIS V VSLQCSA +PC+ +KLE+ID++F GS  K+  FGNSCLNAK
Sbjct: 359 KISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQP-FGNSCLNAK 418

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 419 ITTIGKQNPPAC 428

[Close]

BLAST of MC09g0584 vs. ExPASy TrEMBL
Match: A0A0A0K9C4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G404800 PE=3 SV=1)

HSP 1 Score: 439 bits (1129), Expect = 9.01e-151
Identity = 207/312 (66.35%), Postives = 263/312 (84.29%), Query Frame = 0


Query: 3   PMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRC 62
           PMTVEIQGTVKATTD+S+YS  +WILFESV  L + G GTFDGQG + WK+NDCS++P+C
Sbjct: 123 PMTVEIQGTVKATTDISQYSSAEWILFESVTGLNLIGRGTFDGQGADTWKYNDCSKHPQC 182

Query: 63  VLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTS 122
           ++   +IK NKV +GL+E ++SVN+KAFH F+   ++I  NNV I+APG SPNTDG+H S
Sbjct: 183 IIPPTSIKFNKVTQGLMEGITSVNSKAFHIFVVLSHNIKINNVHIVAPGNSPNTDGVHIS 242

Query: 123 ESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTV 182
           +++++N+TN+ I TGDDCVSIG G+TNINV N+TC PGHGISVGSLGKY+DEK+V G+ V
Sbjct: 243 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYRDEKEVRGIFV 302

Query: 183 TNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQV 242
           +NCT+RNTTNG+RIKTWA S P QA++I+FQ+I+LD V+NPIIIDQ+YGSK K K PSQV
Sbjct: 303 SNCTIRNTTNGVRIKTWAASPPGQATRITFQNIVLDKVRNPIIIDQNYGSKTK-KSPSQV 362

Query: 243 KISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSCLNAK 302
           K+S+V +KNIRGTTIS V VSLQCSA +PC+G+KLE+ID++F G+  K+  F NSCLNAK
Sbjct: 363 KVSDVQFKNIRGTTISPVAVSLQCSAALPCDGIKLEQIDVAFSGTHLKQP-FANSCLNAK 422

Query: 303 ITTVGKQIPPAC 314
           ITT+GKQ PPAC
Sbjct: 423 ITTIGKQNPPAC 432

[Close]

BLAST of MC09g0584 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 307.4 bits (786), Expect = 1.3e-83
Identity = 154/319 (48.28%), Postives = 218/319 (68.34%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           S +TV + GT+ ATT  S Y+ P+W LFE V++L +TG+GTF G+G+ VWK + C +  +
Sbjct: 107 SKVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQ 166

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
           C L   ++K   +K   +  +SSVNAKAFH FL    +++  N+K+ AP ESPNTDGIH 
Sbjct: 167 CNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHL 226

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
           S ++ ++I ++ I+TGDDCVS+G+G+ N+ V  V C PGHG+SVGSLGKY++E+DV G+ 
Sbjct: 227 SNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIH 286

Query: 182 VTNCTLRNTTNGLRIKTWADSRPTQASKISFQDIILDAVKNPIIIDQSYGSKDKSKPPSQ 241
           V NCT+  T NGLRIKTW  S P++A  I F++II+ +VKNPIIIDQ+YGS+      SQ
Sbjct: 287 VNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSRGGD---SQ 346

Query: 242 VKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFV---GSEKKKAA---FG 301
           V IS++ +KNIRGTTI+   V + CS  VPC+GV + ++++ +V   G EKK ++    G
Sbjct: 347 VAISDILFKNIRGTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVG 406

Query: 302 NSCLNAKITTVGKQIPPAC 315
             C NA +   GK   P C
Sbjct: 407 ALCDNANVIFGGKLSFPMC 420

[Close]

BLAST of MC09g0584 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 1.7e-78
Identity = 152/315 (48.25%), Postives = 208/315 (66.03%), Query Frame = 0


Query: 12  VKATTDLSEY-SGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPRCVLLAHTIK 71
           VKA+TDLS+Y SG  WI F  +N LT+TG GTFDGQG   W FN+C+ +  C LL  ++K
Sbjct: 99  VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNCKLLPTSLK 158

Query: 72  LNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHTSESNLINIT 131
              + + +V  +SSVN+K FH  L  C D     + I AP +SPNTDGIH   S+ +  +
Sbjct: 159 FVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIERSSNVYFS 218

Query: 132 NTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVTVTNCTLRNT 191
            ++I+TGDDCVSIGQG + I + ++ C PGHGISVGSLG+Y +EKDV G+ V +C +  T
Sbjct: 219 RSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVKDCKISGT 278

Query: 192 TNGLRIKTWADSRP-TQASKISFQDIILDAVKNPIIIDQSY--GSKDKSKPPSQVKISEV 251
           TNG+RIKTWA+S   + A+ ++F++II++ V NPIIIDQSY   S   S  PS+V++SE+
Sbjct: 279 TNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPSKVELSEI 338

Query: 252 NYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDM---SFVGSEKKKAAFGN-----SCL 311
            +KNIRGT+ S+V V L CS  +PC+ V LE + +   S  G  K+ +  GN     SC 
Sbjct: 339 YFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQSSNRGNEAVSSSCR 398

Query: 312 NAKITTVGKQIPPAC 315
           N +   +G QIPP C
Sbjct: 399 NVRANYIGTQIPPPC 413

[Close]

BLAST of MC09g0584 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 136/317 (42.90%), Postives = 198/317 (62.46%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPD-WILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNP 61
           +P+ + +QGT+KA  D     G + W++F  +N   + G G FDG+G   W+ N+C +  
Sbjct: 76  APVEINLQGTLKA--DGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAAWRVNNCHKTF 135

Query: 62  RCVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIH 121
            C  L  +I+ + V+   +  +SS++AK FH  +    +++F+NVK+IAP ESPNTDGIH
Sbjct: 136 ECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIH 195

Query: 122 TSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGV 181
              S  + I N+ I+TGDDC+S+G G  N++V NV C PGHGISVGSLG+Y  E+DV G+
Sbjct: 196 LGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGI 255

Query: 182 TVTNCTLRNTTNGLRIKTWADSR-PTQASKISFQDIILDAVKNPIIIDQSY--GSKDKSK 241
           TV NCTL+ T NGLRIKTW  +   T AS I F++IIL+ V NPI+IDQ Y   ++   +
Sbjct: 256 TVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQ 315

Query: 242 PPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNS 301
            PS +K+ ++++KNIRGT+ +   V L CS   PC  V++  I++ + G++         
Sbjct: 316 KPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGPPTFM--- 375

Query: 302 CLNAKITTVGKQIPPAC 315
           C N     VG Q P AC
Sbjct: 376 CSNVSPKLVGTQNPKAC 387

[Close]

BLAST of MC09g0584 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.5e-71
Identity = 136/317 (42.90%), Postives = 200/317 (63.09%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPD-WILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNP 61
           +P+ V +QGTVKA  D +   G + W++F +++   + G G FDG+G   W+ N+C +  
Sbjct: 75  APIEVTLQGTVKA--DGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTF 134

Query: 62  RCVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIH 121
            C  L  +I+ + +    +  +SS++AK FH  +    +++ NN+KI+AP +SPNTDGIH
Sbjct: 135 ECKKLPISIRFDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIH 194

Query: 122 TSESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGV 181
              S+ + I N++ISTGDDC+S+G G  N++V  VTC PGHGISVGSLG+Y  E+DV G+
Sbjct: 195 LGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGI 254

Query: 182 TVTNCTLRNTTNGLRIKTWADSR-PTQASKISFQDIILDAVKNPIIIDQSY--GSKDKSK 241
            V NCTL+ T NGLRIKTW  +   T AS I F+DIIL  V NPI+IDQ Y   ++   +
Sbjct: 255 KVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQ 314

Query: 242 PPSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNS 301
             S +K+  +++KNIRGT+ +   V L CS   PC+ V++ +ID+ + G++   A F   
Sbjct: 315 KASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGAD-GPATF--H 374

Query: 302 CLNAKITTVGKQIPPAC 315
           C N     +G Q P AC
Sbjct: 375 CSNVSPKILGSQSPKAC 386

[Close]

BLAST of MC09g0584 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 2.2e-70
Identity = 133/316 (42.09%), Postives = 197/316 (62.34%), Query Frame = 0


Query: 2   SPMTVEIQGTVKATTDLSEYSGPDWILFESVNDLTITGSGTFDGQGQEVWKFNDCSQNPR 61
           +P+ + + GTVKA  D +      W+ F ++N   + G G FDG+G   W+ N+C +   
Sbjct: 76  APIRIALLGTVKA--DGNANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFN 135

Query: 62  CVLLAHTIKLNKVKKGLVEHVSSVNAKAFHFFLFACNDISFNNVKIIAPGESPNTDGIHT 121
           C  L  +I+ + V    +  ++S++AK FH  +    +++F +VKIIAP ESPNTDGIH 
Sbjct: 136 CKKLPISIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHV 195

Query: 122 SESNLINITNTYISTGDDCVSIGQGTTNINVHNVTCAPGHGISVGSLGKYQDEKDVVGVT 181
             S+ I I N++ISTGDDCVS+G G  N+ V  VTC PGHGIS+GSLG+Y  E++V G+ 
Sbjct: 196 GRSDGIKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIK 255

Query: 182 VTNCTLRNTTNGLRIKTWADSR-PTQASKISFQDIILDAVKNPIIIDQSY--GSKDKSKP 241
           + NCTL+ T NGLRIKTW  +   T AS I F++I+L  V NPI+IDQ Y   ++   + 
Sbjct: 256 IINCTLQETDNGLRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQK 315

Query: 242 PSQVKISEVNYKNIRGTTISVVPVSLQCSAKVPCEGVKLEEIDMSFVGSEKKKAAFGNSC 301
           PS +K++ +++K IRGT+ +   V L CS   PC+ V++ ++++ + G++   A F   C
Sbjct: 316 PSTIKLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGAD-GPATF--QC 375

Query: 302 LNAKITTVGKQIPPAC 315
            N     VG QIP AC
Sbjct: 376 SNVSPKLVGTQIPKAC 386

The following BLAST results are available for this feature:

BLAST of MC09g0584 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49062	1.9e-82	48.28	Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ...	[more]
Q6H9K0	1.2e-76	43.27	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P24548	1.7e-75	44.94	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
W8P8Q3	6.7e-72	42.32	Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 P...	[more]
Q39766	3.3e-71	42.22	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]

BLAST of MC09g0584 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146404.1	4.94e-211	91.05	exopolygalacturonase-like [Momordica charantia]	[more]
KAA0046656.1	1.41e-151	67.31	exopolygalacturonase clone [Cucumis melo var. makuwa]	[more]
XP_008451547.1	1.41e-151	67.31	PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo] >TYK18191.1 exopoly...	[more]
XP_011659369.1	1.86e-150	66.35	exopolygalacturonase clone GBGE184 [Cucumis sativus] >KGN44972.1 hypothetical pr...	[more]
XP_038899827.1	3.62e-150	67.95	exopolygalacturonase clone GBGE184 [Benincasa hispida]	[more]

BLAST of MC09g0584 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY21	2.39e-211	91.05	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111015625 PE=3 SV...	[more]
A0A5D3D1R6	6.81e-152	67.31	Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411...	[more]
A0A5A7TXI9	6.81e-152	67.31	Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BRS3	6.81e-152	67.31	exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 ...	[more]
A0A0A0K9C4	9.01e-151	66.35	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G404800 PE=3 SV=1	[more]

BLAST of MC09g0584 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02790.1	1.3e-83	48.28	polygalacturonase 4	[more]
AT4G18180.1	1.7e-78	48.25	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	1.2e-71	42.90	Pectin lyase-like superfamily protein	[more]
AT3G07820.1	4.5e-71	42.90	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	2.2e-70	42.09	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 177..198 e-value: 0.5 score: 19.4 coord: 97..123 e-value: 1600.0 score: 5.8 coord: 124..145 e-value: 1800.0 score: 5.4 coord: 147..167 e-value: 7600.0 score: 0.9
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 2..290 e-value: 1.1E-87 score: 294.1
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 154..167
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..311 e-value: 4.0E-88 score: 298.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..311
None	No IPR available	PANTHER	PTHR31375:SF117	EXOPOLYGALACTURONASE-LIKE	coord: 2..311
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 4..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0584.1	MC09g0584.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity