MC09g0468 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0468
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncation/H(+) antiporter 28
LocationMC09: 4139186 .. 4143195 (-)
RNA-Seq ExpressionMC09g0468
SyntenyMC09g0468
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGGGTAAATTTAGACGAGTGTTATCAAGTCGAGGGGTAAGAAAGAGATTTCACCAAGAAATCAATGTATGAGATCATTAATATGGAAAATTGCATTCTCGTCCCTATATTGTTCAACAATTGCAAGCAATCAACCATGCATTGCATATTGCATATCAAGAGCTGAAAACTAAAAACAAAAATTGGTGTTTGATTTTACCTCGGTGGGATGGGAAATGGAAGTATGTGGTTTTGATCTCTTGCATGGCTCTCCCTCCTGAGACTGCTCCTTCATTTTCTGCTTAAATTCAGCAGATTATCCCAGCCACTCGAACATCCCTGTTGGATCAAAGATGGTGCAATCGTTTTATCATTTTCCAAGGAAAAAGAATAACAGGGAGAGAGAGAGAGAAGAAGGAAAACGAGGGGGGGAATTCTGTTTCTTCGTCTTCTCGCAGCAGAAAAGGGATTTTATGGTGATTGGGAATGCCGCAAGGAAGAAGAAGAAGAAGCGCAATGGCGGGGAGCAGGGTTCGTGAGCCATCGCGATTGTTAGCGGACCATACGTGCACGATTCGGCTCTCTGAAATTTTCGGGAAGGCGGCGAGGTATATTTTGGGCTACTTTCTCATGGCTGGCCTTTGCAATGGCGTGCATTTCCTTTTGAGGCCCTTTTCTCAACCTCGCATAGCCTCCGATACCATCGTAAGTCTCGTCTCCTCTCCGCAAAATTGATATCGATTCTTTGAGAATTTTCGGATCAGACAGAAATTTTGTGTCTGGCTTGGCAATGTAATCTGATGCTTGAGATTTTTTGGGTGTTTCGAAATTCACATGAGTAGGTAGAGCAGTGAAATGGGTATTAGGAAATGGGGGAACTCTGACCTTTTATCTCTCTTTCTTCTTAGGTCAGAATTTCTCAATTGTTTTGGATCCTCTAGAGGCCAATTTGATAGCTATATCTGCCATAAGTAGAATCTGAACCTTATATTTTCTCTCTAGATGGGTAATCAGTGTCTTTAAGAGTGACTTCTTCTTTTCAAGTCCCACGAATAACACTGAAATGGGTGTTGTTAAGGCATTGAAGTTGTACTCATAATCATACCAACAAAGTCTTGCATCTCAAGCAGGAATTAGAAGTCTCGGATGGGCTTTTTTTTTTTTCCAATCAAAAACTATGCTAACCGGCTTGCCTTCTTGGTCTTAGTTTGTATATGCTCATGATTCCTGATTTTCTTCATTTAACACGCAGGTAGGTTTGGTTGTTGGCAACTTCATAAGGAAACAACTTGATATATCGACGATTAAGACGTTGCGTTATATAGTTGACTTCGGTATGACATGCTACATGTTTGTGCTGGGACTGGAGATGGATCCATATGTGCTCTTCAAAGCACCTGCACGCGATGCTAAAGTAGCTTATGCTGGAATGATTTCGACATTCATCTTAGCTGGCTCCATTACCCCATTTATCAGCTTTTCAAAGGACAGAGAGATCAGTTTCATCCTCTCCCTCTCAACTGCTCTCTCTAGCACAGCCTCTCCCGTGCTAACTCGTTTGATCACCAGTCTTAAAATAGGCAAATCAGACATAGGCCGGCTTGTTATTTCTGCTGGGATGCATTCTGATTTTATATCCACTCTTATGATCTGTGTCGGTTATCTCTTTTGTGATTGCCAAGAGACGCGCATCTCCTTCATCAAAGGTTTACGATTAGCTGTTGTGTTATTGATCCAGACAGTACTCACAGCAAAGGTTTCACCGGTATTCATGAACTGGGTAAATAATGAAAACCCCGAGGGTAAACCGATGAAAGGTCCTCACCTGGTTCTTGCAATTGCTTTCATGGCCTCCATTTGTTGCTGCCCTACTATATTTGGCTACAATCCAATTCTCAGTGCATACTTGGCTGGAATATTTTTACCTCGAGAAGGCAGAGTATCAAGGTGGGCAATCGGAAAGATTAACTACCTACTAGTTACCGTTTTCTATCCCATATTCTTCTTTTGGATGGGAGTTGAGTCCAAGTTAAGTGACTTTGAACCTGGACAAATAATGACATGGGTCAGGTTGATTGTGCTTTTTGCTATTGCAACTGTTGGAAAAGTTGTTGGTACCATCATTGCTGGGGCAATTTTGGGGTTTCATTGGCCTGAATCAGTTGCACTTGGGCTGCTGTTAACCATGAAGGGCCATTTTCATATATACTTTGCCATTGCTGCGAAAACAGTAAGTACCCTTGCTCGAGGCTTCATCGTTTTCTACCAATGTATTTTCATCTTGATAAATACAGCACTAAAATCGAACTATTTCACCTTATAAATGATGAACAAATGCAGGCTGGAAAGACGACTACATCTACGAGCACTGTGATGGTAATCGTGGTCTTCTTCACAGTCGTGCATGCCCCGCAAGTTGTGGCACATATAATCAAACGGGCAAGGAAACGAACACCTACGCATCGAATGGCACTCCAATTGCTTGATCCAGCAAGTGAGCTTAAGATCTTGTTATGCATACATGGACCTCAGAACACAGCCGCAGCCATTAACATCATGGAGATTTCACGGGGGGCAGCTAACCCTGGAGTTGTTGTTTATGTCACAGACATGATTGAACTTACAGATGAAATAGCAGCCACATTAGTGCAAGGTGAAGGAGTGGACTCCGTGACGGTAACCCACACAGGAGTGACACAAATGAGAGACCAAGTTACTGCTGCTGTCCAGAGCTATGTAGACGAAAATGGAGAAGGAATTACCTTCAGAAGAATGTTGGCTCTCTCAACACTCAATAGTATGGCTCAGGACATTTGCATTTTAGCAGAGGAATTGATGGGAGCTCTTATCATACTGCCATTCCACAAGAGCCAGCGTGGAAATGGGGCGTTGGGTGAGGGCCATTCCGGTTTCCGTTTTGTAAACCGCAAGGTAATGATACTAAAAGACTGAAAGTTCTGCACAGTCAGTTATAACTGGTTCCGTTCAAGTATATTAGTATTGATGCTAGTAGAACATATACAGTTACAAGGGAATTGATTGATTATAGGATGTGTAGTGCAAATAAAGTGAAGATTTTGATCGATTAAGTTTAGAGAATTTGTTGTTTCATATAAATTTTGTCACCAATGCTTCTCATGCATCAGTAGCTGACACATTTCATTCAAACAGGTTCTCAGGAATGCCCCCTGCTCTGTGGGAATCCTAGTGGATCGAGGTCTTGGAACGGTAGAAAAAATTTCAAGGTCGTATGTATCTCAGAATGTGGCAGTCATCTTCATTGGCGGTAAAGACGACAGAGAAGCATTATACTATGCAGGCCGAGTAGCAAAGCATCCAGGAGTTAAACTCTCAGTGATTAGATTCTTGGTAGATGCTGATGCGGAAAATGCTGCAAGAAGAGCTGGGACTTACAGGATAAGTGAAGCTGAGCAAGAAGAGGAAATGAGACAAGACGATGAATGCTTTGCTTATTTTTACGAACGGTATGTGGCCGGTGGGCATGTTGCTTATGTAGAAAAACACCTTGCCAGTTCCTCCGAGACGTATTCTACTTTGAAATCGCTGGATGGGCAATATGCACTCGTAATTGTCGGTCGAGGTGGGGGAGTCAACTCGGTGTTAACTTTTGGGATGAATGACTGGCAACAATGTCCGGAATTGGGTCCTATAGGGGATGTTCTTTCTGGATCTGAATTCGCTGTTAGGACCTCGGTTTTGATCATCCAACAACACAATCTAAAGGGAGAACTAGATGGACTTGATGATGATTTTTCAATTATGTAGATGTCAAGAAACCAATGAGATTAGTAGTGATAGACATTTAGAAGCTAATTGTAGCCATGAATAACAGTGATTTGTAGAGAAGTTTAGTTGATGGAAATGATAGAAAGTGTGTAGAAATTGACAGGTATATAGACTTCTGACCATTTGACTTGTTCTGTAAAACAGTGATTTCTAACCAATTGAGAATCGAATCAAGTTCTCATTTATATTTTACTGCTATATATGTGAGTCGAAATTGGTGCCTT

mRNA sequence

TAGGGTAAATTTAGACGAGTGTTATCAAGTCGAGGGGTAAGAAAGAGATTTCACCAAGAAATCAATGTATGAGATCATTAATATGGAAAATTGCATTCTCGTCCCTATATTGTTCAACAATTGCAAGCAATCAACCATGCATTGCATATTGCATATCAAGAGCTGAAAACTAAAAACAAAAATTGGTGTTTGATTTTACCTCGGTGGGATGGGAAATGGAAGTATGTGGTTTTGATCTCTTGCATGGCTCTCCCTCCTGAGACTGCTCCTTCATTTTCTGCTTAAATTCAGCAGATTATCCCAGCCACTCGAACATCCCTGTTGGATCAAAGATGGTGCAATCGTTTTATCATTTTCCAAGGAAAAAGAATAACAGGGAGAGAGAGAGAGAAGAAGGAAAACGAGGGGGGGAATTCTGTTTCTTCGTCTTCTCGCAGCAGAAAAGGGATTTTATGGTGATTGGGAATGCCGCAAGGAAGAAGAAGAAGAAGCGCAATGGCGGGGAGCAGGGTTCGTGAGCCATCGCGATTGTTAGCGGACCATACGTGCACGATTCGGCTCTCTGAAATTTTCGGGAAGGCGGCGAGGTATATTTTGGGCTACTTTCTCATGGCTGGCCTTTGCAATGGCGTGCATTTCCTTTTGAGGCCCTTTTCTCAACCTCGCATAGCCTCCGATACCATCGTAGGTTTGGTTGTTGGCAACTTCATAAGGAAACAACTTGATATATCGACGATTAAGACGTTGCGTTATATAGTTGACTTCGGTATGACATGCTACATGTTTGTGCTGGGACTGGAGATGGATCCATATGTGCTCTTCAAAGCACCTGCACGCGATGCTAAAGTAGCTTATGCTGGAATGATTTCGACATTCATCTTAGCTGGCTCCATTACCCCATTTATCAGCTTTTCAAAGGACAGAGAGATCAGTTTCATCCTCTCCCTCTCAACTGCTCTCTCTAGCACAGCCTCTCCCGTGCTAACTCGTTTGATCACCAGTCTTAAAATAGGCAAATCAGACATAGGCCGGCTTGTTATTTCTGCTGGGATGCATTCTGATTTTATATCCACTCTTATGATCTGTGTCGGTTATCTCTTTTGTGATTGCCAAGAGACGCGCATCTCCTTCATCAAAGGTTTACGATTAGCTGTTGTGTTATTGATCCAGACAGTACTCACAGCAAAGGTTTCACCGGTATTCATGAACTGGGTAAATAATGAAAACCCCGAGGGTAAACCGATGAAAGGTCCTCACCTGGTTCTTGCAATTGCTTTCATGGCCTCCATTTGTTGCTGCCCTACTATATTTGGCTACAATCCAATTCTCAGTGCATACTTGGCTGGAATATTTTTACCTCGAGAAGGCAGAGTATCAAGGTGGGCAATCGGAAAGATTAACTACCTACTAGTTACCGTTTTCTATCCCATATTCTTCTTTTGGATGGGAGTTGAGTCCAAGTTAAGTGACTTTGAACCTGGACAAATAATGACATGGGTCAGGTTGATTGTGCTTTTTGCTATTGCAACTGTTGGAAAAGTTGTTGGTACCATCATTGCTGGGGCAATTTTGGGGTTTCATTGGCCTGAATCAGTTGCACTTGGGCTGCTGTTAACCATGAAGGGCCATTTTCATATATACTTTGCCATTGCTGCGAAAACAGCTGGAAAGACGACTACATCTACGAGCACTGTGATGGTAATCGTGGTCTTCTTCACAGTCGTGCATGCCCCGCAAGTTGTGGCACATATAATCAAACGGGCAAGGAAACGAACACCTACGCATCGAATGGCACTCCAATTGCTTGATCCAGCAAGTGAGCTTAAGATCTTGTTATGCATACATGGACCTCAGAACACAGCCGCAGCCATTAACATCATGGAGATTTCACGGGGGGCAGCTAACCCTGGAGTTGTTGTTTATGTCACAGACATGATTGAACTTACAGATGAAATAGCAGCCACATTAGTGCAAGGTGAAGGAGTGGACTCCGTGACGGTAACCCACACAGGAGTGACACAAATGAGAGACCAAGTTACTGCTGCTGTCCAGAGCTATGTAGACGAAAATGGAGAAGGAATTACCTTCAGAAGAATGTTGGCTCTCTCAACACTCAATAGTATGGCTCAGGACATTTGCATTTTAGCAGAGGAATTGATGGGAGCTCTTATCATACTGCCATTCCACAAGAGCCAGCGTGGAAATGGGGCGTTGGGTGAGGGCCATTCCGGTTTCCGTTTTGTAAACCGCAAGGTTCTCAGGAATGCCCCCTGCTCTGTGGGAATCCTAGTGGATCGAGGTCTTGGAACGGTAGAAAAAATTTCAAGGTCGTATGTATCTCAGAATGTGGCAGTCATCTTCATTGGCGGTAAAGACGACAGAGAAGCATTATACTATGCAGGCCGAGTAGCAAAGCATCCAGGAGTTAAACTCTCAGTGATTAGATTCTTGGTAGATGCTGATGCGGAAAATGCTGCAAGAAGAGCTGGGACTTACAGGATAAGTGAAGCTGAGCAAGAAGAGGAAATGAGACAAGACGATGAATGCTTTGCTTATTTTTACGAACGGTATGTGGCCGGTGGGCATGTTGCTTATGTAGAAAAACACCTTGCCAGTTCCTCCGAGACGTATTCTACTTTGAAATCGCTGGATGGGCAATATGCACTCGTAATTGTCGGTCGAGGTGGGGGAGTCAACTCGGTGTTAACTTTTGGGATGAATGACTGGCAACAATGTCCGGAATTGGGTCCTATAGGGGATGTTCTTTCTGGATCTGAATTCGCTGTTAGGACCTCGGTTTTGATCATCCAACAACACAATCTAAAGGGAGAACTAGATGGACTTGATGATGATTTTTCAATTATGTAGATGTCAAGAAACCAATGAGATTAGTAGTGATAGACATTTAGAAGCTAATTGTAGCCATGAATAACAGTGATTTGTAGAGAAGTTTAGTTGATGGAAATGATAGAAAGTGTGTAGAAATTGACAGGTATATAGACTTCTGACCATTTGACTTGTTCTGTAAAACAGTGATTTCTAACCAATTGAGAATCGAATCAAGTTCTCATTTATATTTTACTGCTATATATGTGAGTCGAAATTGGTGCCTT

Coding sequence (CDS)

ATGCCGCAAGGAAGAAGAAGAAGAAGCGCAATGGCGGGGAGCAGGGTTCGTGAGCCATCGCGATTGTTAGCGGACCATACGTGCACGATTCGGCTCTCTGAAATTTTCGGGAAGGCGGCGAGGTATATTTTGGGCTACTTTCTCATGGCTGGCCTTTGCAATGGCGTGCATTTCCTTTTGAGGCCCTTTTCTCAACCTCGCATAGCCTCCGATACCATCGTAGGTTTGGTTGTTGGCAACTTCATAAGGAAACAACTTGATATATCGACGATTAAGACGTTGCGTTATATAGTTGACTTCGGTATGACATGCTACATGTTTGTGCTGGGACTGGAGATGGATCCATATGTGCTCTTCAAAGCACCTGCACGCGATGCTAAAGTAGCTTATGCTGGAATGATTTCGACATTCATCTTAGCTGGCTCCATTACCCCATTTATCAGCTTTTCAAAGGACAGAGAGATCAGTTTCATCCTCTCCCTCTCAACTGCTCTCTCTAGCACAGCCTCTCCCGTGCTAACTCGTTTGATCACCAGTCTTAAAATAGGCAAATCAGACATAGGCCGGCTTGTTATTTCTGCTGGGATGCATTCTGATTTTATATCCACTCTTATGATCTGTGTCGGTTATCTCTTTTGTGATTGCCAAGAGACGCGCATCTCCTTCATCAAAGGTTTACGATTAGCTGTTGTGTTATTGATCCAGACAGTACTCACAGCAAAGGTTTCACCGGTATTCATGAACTGGGTAAATAATGAAAACCCCGAGGGTAAACCGATGAAAGGTCCTCACCTGGTTCTTGCAATTGCTTTCATGGCCTCCATTTGTTGCTGCCCTACTATATTTGGCTACAATCCAATTCTCAGTGCATACTTGGCTGGAATATTTTTACCTCGAGAAGGCAGAGTATCAAGGTGGGCAATCGGAAAGATTAACTACCTACTAGTTACCGTTTTCTATCCCATATTCTTCTTTTGGATGGGAGTTGAGTCCAAGTTAAGTGACTTTGAACCTGGACAAATAATGACATGGGTCAGGTTGATTGTGCTTTTTGCTATTGCAACTGTTGGAAAAGTTGTTGGTACCATCATTGCTGGGGCAATTTTGGGGTTTCATTGGCCTGAATCAGTTGCACTTGGGCTGCTGTTAACCATGAAGGGCCATTTTCATATATACTTTGCCATTGCTGCGAAAACAGCTGGAAAGACGACTACATCTACGAGCACTGTGATGGTAATCGTGGTCTTCTTCACAGTCGTGCATGCCCCGCAAGTTGTGGCACATATAATCAAACGGGCAAGGAAACGAACACCTACGCATCGAATGGCACTCCAATTGCTTGATCCAGCAAGTGAGCTTAAGATCTTGTTATGCATACATGGACCTCAGAACACAGCCGCAGCCATTAACATCATGGAGATTTCACGGGGGGCAGCTAACCCTGGAGTTGTTGTTTATGTCACAGACATGATTGAACTTACAGATGAAATAGCAGCCACATTAGTGCAAGGTGAAGGAGTGGACTCCGTGACGGTAACCCACACAGGAGTGACACAAATGAGAGACCAAGTTACTGCTGCTGTCCAGAGCTATGTAGACGAAAATGGAGAAGGAATTACCTTCAGAAGAATGTTGGCTCTCTCAACACTCAATAGTATGGCTCAGGACATTTGCATTTTAGCAGAGGAATTGATGGGAGCTCTTATCATACTGCCATTCCACAAGAGCCAGCGTGGAAATGGGGCGTTGGGTGAGGGCCATTCCGGTTTCCGTTTTGTAAACCGCAAGGTTCTCAGGAATGCCCCCTGCTCTGTGGGAATCCTAGTGGATCGAGGTCTTGGAACGGTAGAAAAAATTTCAAGGTCGTATGTATCTCAGAATGTGGCAGTCATCTTCATTGGCGGTAAAGACGACAGAGAAGCATTATACTATGCAGGCCGAGTAGCAAAGCATCCAGGAGTTAAACTCTCAGTGATTAGATTCTTGGTAGATGCTGATGCGGAAAATGCTGCAAGAAGAGCTGGGACTTACAGGATAAGTGAAGCTGAGCAAGAAGAGGAAATGAGACAAGACGATGAATGCTTTGCTTATTTTTACGAACGGTATGTGGCCGGTGGGCATGTTGCTTATGTAGAAAAACACCTTGCCAGTTCCTCCGAGACGTATTCTACTTTGAAATCGCTGGATGGGCAATATGCACTCGTAATTGTCGGTCGAGGTGGGGGAGTCAACTCGGTGTTAACTTTTGGGATGAATGACTGGCAACAATGTCCGGAATTGGGTCCTATAGGGGATGTTCTTTCTGGATCTGAATTCGCTGTTAGGACCTCGGTTTTGATCATCCAACAACACAATCTAAAGGGAGAACTAGATGGACTTGATGATGATTTTTCAATTATGTAG

Protein sequence

MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGELDGLDDDFSIM
Homology
BLAST of MC09g0468 vs. ExPASy Swiss-Prot
Match: Q8L709 (Cation/H(+) antiporter 28 OS=Arabidopsis thaliana OX=3702 GN=CHX28 PE=2 SV=2)

HSP 1 Score: 806.2 bits (2081), Expect = 3.3e-232
Identity = 415/762 (54.46%), Postives = 544/762 (71.39%), Query Frame = 0

Query: 56  VHFLLRPFSQPRIASDTIVGLVVGNFIRKQLDIS--TIKTLRYIVDFGMTCYMFVLGLEM 115
           +H+L++P  QP + +D  +GL++GN  R +   S     TL  I++FGM C+MFV+GLEM
Sbjct: 40  LHYLMKPLGQPYLTTDFAIGLILGNIPRFRGAFSGPYSITLNNIIEFGMICHMFVMGLEM 99

Query: 116 DPYVLFKAPARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVL 175
           +P VL + P +DA +AY  MI+TF+LA   TPF+ ++K     F L+LS   SST SP+L
Sbjct: 100 NPSVLLRPPTKDAFIAYTSMITTFVLAFVTTPFLHYTKTSPYIFSLALSLMASSTGSPIL 159

Query: 176 TRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRI----SFIKGLRLA 235
           TR+I +LKI KSD+G+L  +AG+H+D ISTL+ C G++F   ++        F + L + 
Sbjct: 160 TRVIANLKIRKSDLGKLASAAGVHTDMISTLLYCFGFIFFPTEKPLARPLHRFFRALLMF 219

Query: 236 VVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPT---IFGYNP 295
            + L Q   T+ VSP+F+NWVNNENPEGKP+KG HLV+++AF+  IC  PT      YNP
Sbjct: 220 CLFLAQVTFTSIVSPIFLNWVNNENPEGKPLKGSHLVMSLAFVVLICSFPTWPPESMYNP 279

Query: 296 ILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVR 355
           ILSA+ AG+FLP +GR+S+W I KINYLL TVFYPIFFFW+G    + +F+    M WVR
Sbjct: 280 ILSAFTAGLFLPNKGRMSKWIINKINYLLSTVFYPIFFFWVGFIIHMRNFDITDKMAWVR 339

Query: 356 LIVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTS 415
              L      GKV GT++ G +LG+H PE+ +LGLLLT KGHFH+Y A  A    +  ++
Sbjct: 340 FFSLLGTVIAGKVTGTVLCGLLLGYHVPETASLGLLLTTKGHFHVYLAALAIRTNRVKST 399

Query: 416 TSTVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTA 475
           T  +++ ++ FTVV++P VV  IIKRARKR P H MALQ LDP +EL+IL+ +HGP N  
Sbjct: 400 TGALIIFIIVFTVVYSPFVVMDIIKRARKRVPVHIMALQWLDPTTELRILIGLHGPHNIG 459

Query: 476 AAINIMEISRGAANPGVVVYVTDMIELTDEIAATLVQGEGV----DSVTVTHTGVTQMRD 535
           + +N+MEI  G   PG + Y TDM+ELTDEIAATL +G G     DSVTVT   VT+MR+
Sbjct: 460 STLNVMEICHGGREPGSIFYATDMVELTDEIAATLKKGGGAGQSNDSVTVTDRSVTEMRE 519

Query: 536 QVTAAVQSYVD-ENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNG 595
            +TAAV  Y +  NG+G+T RRMLALST  +MA D+C LA+ELM ++IILPFHK    +G
Sbjct: 520 SITAAVNGYGELRNGQGVTVRRMLALSTFVTMAHDVCGLADELMVSIIILPFHKRLNPDG 579

Query: 596 ALGEGHSGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREA 655
            L  GH+GFR VNRK+L+NAPCSVGILVDR  G  E+  R   S  +A+IFIGG+DDREA
Sbjct: 580 TLDAGHAGFRHVNRKILKNAPCSVGILVDRSFGQTEEAWRPGASMGIAIIFIGGRDDREA 639

Query: 656 LYYAGRVAKHPGVKLSVIRFLVDADAENAARRAGTY-RISEAEQEEEMRQDDECFAYFYE 715
           L +A +VA+HP VKL VIRFL D  ++NA +R+    R S  +QEEEM+ DDECFA FYE
Sbjct: 640 LAFAAQVARHPAVKLKVIRFLEDKSSQNAQKRSSILNRASVVDQEEEMKLDDECFAEFYE 699

Query: 716 RYVA-GGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGG-VNSVLTFGMNDWQQCP 775
           RY+A GG V+Y+EKHL +SSET++ LKSLDG+Y LVIVGRGGG  +S LT G+NDWQQCP
Sbjct: 700 RYIAGGGRVSYMEKHLTNSSETFTALKSLDGEYGLVIVGRGGGRASSGLTTGLNDWQQCP 759

Query: 776 ELGPIGDVLSGSEFAVRTSVLIIQQHNLKGELDGLDDDFSIM 801
           ELGPIGDVLSGS+F+  TS+LIIQQ   +G+L+GL DDF+I+
Sbjct: 760 ELGPIGDVLSGSDFSHNTSMLIIQQQRTRGQLEGLHDDFTIL 801

BLAST of MC09g0468 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 9.3e-102
Identity = 226/741 (30.50%), Postives = 391/741 (52.77%), Query Frame = 0

Query: 58  FLLRPFSQPRIASDTIVGLVVG--------NFIRKQLDISTIKTLRYIVDFGMTCYMFVL 117
           F+L+PF QPR+ S+ + G+V+G         F        ++  L  + + G+  ++F++
Sbjct: 56  FILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLV 115

Query: 118 GLEMDPYVLFKAPARDAKVAYAGMISTFILAGSITPFISFSKDR--EISFILSLSTALSS 177
           G+EMD  V+ K   R   +A  GM+  F++  + +  +  S+D   + ++IL L  ALS 
Sbjct: 116 GVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSV 175

Query: 178 TASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLR 237
           TA PVL R++  LK+  ++IGR+ +SA + +D  + +++ +     +  +T  + +  + 
Sbjct: 176 TAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMI 235

Query: 238 LAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPI 297
            + V +   V   +     + W+  + PEG+     H+ L +  +          G + +
Sbjct: 236 SSAVFIAVCVFVVRPG---IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSV 295

Query: 298 LSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRL 357
             A++ G+ +P  G +    I K+   +  +  P+FF   G+++ ++  +     TW+ L
Sbjct: 296 FGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGP--ATWLTL 355

Query: 358 IVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTST 417
            ++  +A  GKV+GT+I     G    E + LGLLL  KG   +      K        T
Sbjct: 356 FLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDET 415

Query: 418 STVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAA 477
              MV+V          +V  + K  +K     R  +Q   P SEL++L+C+H P+N   
Sbjct: 416 FATMVLVALVMTGVITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPT 475

Query: 478 AINIMEISRGAANPGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAA 537
            IN++E S       + +YV  ++ELT   +A L+      S            D +  A
Sbjct: 476 IINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINA 535

Query: 538 VQSYVDENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGH 597
            ++Y +++   +  + + A+S  ++M +D+C LAE+   + II+PFHK Q  +G +   +
Sbjct: 536 FENY-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTN 595

Query: 598 SGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGR 657
             +R VN+ +L N+PCSVGILVDRGL    +++ + VS  VAV+F GG DDREAL YA R
Sbjct: 596 PAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWR 655

Query: 658 VAKHPGVKLSVIRFLVDAD-AENAARRA---GTYRISEAEQEEEMRQDDECFAYFYERYV 717
           +A+HPG+ L+V+RF+ D D A+ A+ RA      +I + +  ++ + DD+    F     
Sbjct: 656 MAQHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENA 715

Query: 718 AGGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPI 777
               + Y+EK +++  ET + ++S+D  + L IVGRG G++S LT G+ DW +CPELG I
Sbjct: 716 EYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAI 775

Query: 778 GDVLSGSEFAVRTSVLIIQQH 785
           GD+L+ S+FA   SVL++QQ+
Sbjct: 776 GDLLASSDFAATVSVLVVQQY 789

BLAST of MC09g0468 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.3e-82
Identity = 220/745 (29.53%), Postives = 379/745 (50.87%), Query Frame = 0

Query: 58  FLLRPFSQPRIASDTIVGLVVGNFI--RKQLDISTI------KTLRYIVDFGMTCYMFVL 117
           + L+P  QPR+ ++ I G+++G     R +  + TI        L  + + G+  ++F++
Sbjct: 51  YFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVLDTLANIGLLFFLFLV 110

Query: 118 GLEMDPYVLFKAPARDAKVAYAGMISTFILAGSITPFI---SFSKD-REISFILSLSTAL 177
           GLE+D   + K   +   +A AG+   FI+ G  T F+   + SK   ++ FI+ +  AL
Sbjct: 111 GLELDFAAIKKTGKKSLLIAIAGISLPFIV-GVGTSFVLSATISKGVDQLPFIVFMGVAL 170

Query: 178 SSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKG 237
           S TA PVL R++  LK+  +DIGR+ +SA   +D  + +++ +         + +  +  
Sbjct: 171 SITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIALSGDGTSPLVSVWV 230

Query: 238 LRLAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYN 297
           L      +I  V+  K     + ++    PEG+P+K  ++ + +  + +        G +
Sbjct: 231 LLCGTGFVIFAVVAIK---PLLAYMARRCPEGEPVKELYVCVTLTVVLAASFVTDTIGIH 290

Query: 298 PILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWV 357
            +  A++ GI  P+EG   R    KI  L+  +  P++F   G+++ ++     Q  +W 
Sbjct: 291 ALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLKTDVTTIRGAQ--SWG 350

Query: 358 RLIVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTT 417
            L+++      GK+VGT+ +  +    + E+V LG L+  KG   +      K       
Sbjct: 351 LLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVELIVLNIGKDRKVLND 410

Query: 418 STSTVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNT 477
               ++V++  FT      +V  I K ARK  P     +Q  D  SEL+IL C H  +N 
Sbjct: 411 QAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHRTIQRKDHDSELRILACFHSTRNI 470

Query: 478 AAAINIMEISRGAANPG-VVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQV 537
              IN++E SRG    G + VY   ++EL++  +A  +  +  ++       + +  DQ+
Sbjct: 471 PTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARNNGLPIWNKIERSTDQM 530

Query: 538 TAAVQSYVDENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALG 597
             A ++Y  ++   +  R M A+S L+S+ +DIC  A +   A+I+LPFHK QR +GA+ 
Sbjct: 531 VIAFEAY--QHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMILLPFHKHQRMDGAME 590

Query: 598 EGHSGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYY 657
                F  VN++VL+ APCSVGILVDRGLG   ++  S V+  V + F GG DDREAL Y
Sbjct: 591 SIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVVIPFFGGLDDREALAY 650

Query: 658 AGRVAKHPGVKLSVIRFLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYV- 717
             ++ +HPG+ L+V +F+        A R    R  ++E +E+ +++ E    F    + 
Sbjct: 651 GMKMVEHPGITLTVYKFV--------AARGTLKRFEKSEHDEKEKKEKETDEEFVRELMN 710

Query: 718 ---AGGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPEL 777
                  +AY E+ + S  +  +TLKS+  +  L +VGR   V S++         CPEL
Sbjct: 711 DPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVVGRNAAVASLVKS-----TDCPEL 770

Query: 778 GPIGDVLSGSEFAVRTSVLIIQQHN 786
           GP+G +LS SEF+   SVL++Q ++
Sbjct: 771 GPVGRLLSSSEFSTTASVLVVQGYD 773

BLAST of MC09g0468 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.8e-81
Identity = 221/777 (28.44%), Postives = 387/777 (49.81%), Query Frame = 0

Query: 43  ILGYFLMAGLCNGVHFLLRPFSQPRIASDTIVGLVVG--------NFIRKQLDISTIKTL 102
           IL   ++  L   + +LLRP  QPR+ ++ I G+++G         F+       ++  L
Sbjct: 35  ILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVL 94

Query: 103 RYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAYAGMI--------STFILAGSITPF 162
             + + G+  ++F+ GLE+D   L +   +   +A AG+         S+F+L  +I+  
Sbjct: 95  ETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISKG 154

Query: 163 ISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMI 222
           ++       +F++ +  ALS TA PVL R++  LK+  ++IGRL +SA   +D  + +++
Sbjct: 155 VN-----STAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILL 214

Query: 223 CVGYLFCDCQETRI----SFIKGLRLAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKG 282
            +         + +     F+ G    +         + + P    W++    EG+P++ 
Sbjct: 215 ALAIALSGSNTSPLVSLWVFLSGCAFVIG-------ASFIIPPIFRWISRRCHEGEPIEE 274

Query: 283 PHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPI 342
            ++   +A +          G + +  A++ G+ +P+EG  +   + K+  L+  +F P+
Sbjct: 275 TYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPL 334

Query: 343 FFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILGFHWP--ESVALG 402
           +F   G+++ ++  +  Q  +W  L+++ A A  GK++GT+  G  L F  P  E++ LG
Sbjct: 335 YFVASGLKTNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLG 394

Query: 403 LLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHIIK---RARKRT 462
            L+  KG   +      K        T  +MV++  FT      VV  + K   RA+K  
Sbjct: 395 FLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEG 454

Query: 463 PTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRG-AANPGVVVYVTDMIELTDE 522
                A++  +  ++L+IL C HG  +  + IN++E SRG     G+ VY   + EL++ 
Sbjct: 455 EYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSER 514

Query: 523 IAATL----VQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTLNS 582
            +A L    V+  G+        GV    DQV  A Q++  +    +  R M A+S+++ 
Sbjct: 515 SSAILMVHKVRKNGMP--FWNRRGVNADADQVVVAFQAF--QQLSRVNVRPMTAISSMSD 574

Query: 583 MAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVDRG 642
           + +DIC  A     A++ILPFHK Q+ +G+L      +R+VNR+VL  APCSVGI VDRG
Sbjct: 575 IHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRG 634

Query: 643 LGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDAD-AENAA 702
           LG   ++S   VS +V V+F GG DDREAL Y  R+A+HPG+ L+V RF+V  +      
Sbjct: 635 LGGSSQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIV 694

Query: 703 RRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDGQ 762
               +   +E +  + ++ D+E  +   +       V +VEK + +++    +      +
Sbjct: 695 NVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVRR 754

Query: 763 YALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKG 789
             L +VGR  G    L    N   +CPELGP+G +L   E + + SVL+IQQ+N  G
Sbjct: 755 SNLFLVGRMPGGEIALAIREN--SECPELGPVGSLLISPESSTKASVLVIQQYNGTG 789

BLAST of MC09g0468 vs. ExPASy Swiss-Prot
Match: Q8VYD4 (Cation/H(+) antiporter 23, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHX23 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 2.0e-67
Identity = 196/748 (26.20%), Postives = 354/748 (47.33%), Query Frame = 0

Query: 56  VHFLLRPFSQPRIASDTIVGL-----VVGN---FIRKQLDISTIKTLRYIVDFGMTCYMF 115
           +++L RP   P   +  + GL     V+GN    I           L    +  +   +F
Sbjct: 68  LYYLTRPLYLPPFVAQILCGLLFSPSVLGNTRFIIAHVFPYRFTMVLETFANLALVYNIF 127

Query: 116 VLGLEMDPYVLFKAPARDAKVAYAGMISTFILAGSITPFI--SFSKDREISFILSLSTAL 175
           +LGL MD  ++     +   +A+ G++   +  G+   ++  +   D+ IS  +  S AL
Sbjct: 128 LLGLGMDLRMVRITELKPVIIAFTGLL-VALPVGAFLYYLPGNGHPDKIISGCVFWSVAL 187

Query: 176 SSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQET---RISF 235
           + T  P L R++  LK+ +SD+GR  + A + +D  + +++  G+       T    + F
Sbjct: 188 ACTNFPDLARILADLKLLRSDMGRTAMCAAIVTDLCTWVLLVFGFASFSKSGTWNKMMPF 247

Query: 236 IKGLRLAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIF 295
           +       VLL   V+   ++     W+  +  +   +   H+   +  +          
Sbjct: 248 VIITTAIFVLLCIFVIRPGIA-----WIFAKTVKAGHVGDTHVWFILGGVVLCGLITDAC 307

Query: 296 GYNPILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIM 355
           G + I  A+L G+ +P +  +      K++  L  +  P+F+   G+ + +      Q  
Sbjct: 308 GVHSITGAFLFGLSIPHDHIIRNMIEEKLHDFLSGILMPLFYIICGLRADIGFML--QFT 367

Query: 356 TWVRLIVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGK 415
               ++V+   + + K+V T+I    +     ++ A+G L+  KG   +    A +    
Sbjct: 368 DKFMMVVVICSSFLVKIVTTVITSLFMHIPMRDAFAIGALMNTKGTLSLVVLNAGRDTKA 427

Query: 416 TTTSTSTVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGP 475
             +   T M I +    +    ++A   K  +K        +Q +   +EL++L C+H  
Sbjct: 428 LDSPMYTHMTIALLVMSLVVEPLLAFAYKPKKKLAHYKHRTVQKIKGETELRVLACVHVL 487

Query: 476 QNTAAAINIMEISRGAANPGVVVYVTDMIELTDEIAAT-LVQGEGVDSVTVTHTGVTQMR 535
            N +   N++++S       + V+   ++ELT    A+ L+  +           V    
Sbjct: 488 PNVSGITNLLQVSNATKQSPLSVFAIHLVELTGRTTASLLIMNDECKPKANFSDRVRAES 547

Query: 536 DQVTAAVQSYVDENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNG 595
           DQ+    ++ ++ N + +T + + A+S   +M +DIC+LAE+     IILP+HK    +G
Sbjct: 548 DQIAETFEA-MEVNNDAMTVQTITAVSPYATMHEDICVLAEDKRVCFIILPYHKHLTPDG 607

Query: 596 ALGEGHSGFRFVNRKVLRNAPCSVGILVDRGLGTV--EKISRSYVSQNVAVIFIGGKDDR 655
            +GEG+S    +N+ VL +APCSVGILVDRG+  V  E      + + VA++F+GG DDR
Sbjct: 608 RMGEGNSSHAEINQNVLSHAPCSVGILVDRGMAMVRSESFRGESMKREVAMLFVGGPDDR 667

Query: 656 EALYYAGRVAKHPGVKLSVIRFLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFY 715
           EAL YA R+     +KL+V+RF+   +A  ++ +        AE E E + DDEC   F 
Sbjct: 668 EALSYAWRMVGQHVIKLTVVRFVPGREALISSGKVA------AEYEREKQVDDECIYEFN 727

Query: 716 ERYVAGGHVAYVEKHLASSSETYSTLKSLD--GQYALVIVGRGGGVNSVLTFGMNDWQQC 775
            + +    V Y+EK +    +T +T++ ++    Y L +VGRG   +S +T G+NDW   
Sbjct: 728 FKTMNDSSVKYIEKVVNDGQDTIATIREMEDNNSYDLYVVGRGYNSDSPVTAGLNDWSSS 787

Query: 776 PELGPIGDVLSGSEFAVRTSVLIIQQHN 786
           PELG IGD L+ S F +  SVL+IQQ++
Sbjct: 788 PELGTIGDTLASSNFTMHASVLVIQQYS 800

BLAST of MC09g0468 vs. NCBI nr
Match: XP_022147855.1 (cation/H(+) antiporter 28 [Momordica charantia])

HSP 1 Score: 1552 bits (4019), Expect = 0.0
Identity = 800/800 (100.00%), Postives = 800/800 (100.00%), Query Frame = 0

Query: 1   MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLL 60
           MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLL
Sbjct: 1   MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLL 60

Query: 61  RPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFK 120
           RPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFK
Sbjct: 61  RPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFK 120

Query: 121 APARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSL 180
           APARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSL
Sbjct: 121 APARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSL 180

Query: 181 KIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTA 240
           KIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTA
Sbjct: 181 KIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTA 240

Query: 241 KVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPRE 300
           KVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPRE
Sbjct: 241 KVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPRE 300

Query: 301 GRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVV 360
           GRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVV
Sbjct: 301 GRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVV 360

Query: 361 GTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVV 420
           GTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVV
Sbjct: 361 GTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVV 420

Query: 421 HAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAAN 480
           HAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAAN
Sbjct: 421 HAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAAN 480

Query: 481 PGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGIT 540
           PGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGIT
Sbjct: 481 PGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGIT 540

Query: 541 FRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRN 600
           FRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRN
Sbjct: 541 FRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRN 600

Query: 601 APCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIR 660
           APCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIR
Sbjct: 601 APCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIR 660

Query: 661 FLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSE 720
           FLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSE
Sbjct: 661 FLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSE 720

Query: 721 TYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLI 780
           TYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLI
Sbjct: 721 TYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLI 780

Query: 781 IQQHNLKGELDGLDDDFSIM 800
           IQQHNLKGELDGLDDDFSIM
Sbjct: 781 IQQHNLKGELDGLDDDFSIM 800

BLAST of MC09g0468 vs. NCBI nr
Match: XP_038896873.1 (cation/H(+) antiporter 28 isoform X2 [Benincasa hispida])

HSP 1 Score: 1410 bits (3649), Expect = 0.0
Identity = 715/798 (89.60%), Postives = 759/798 (95.11%), Query Frame = 0

Query: 3   QGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRP 62
           +GRRRR+AMA +R REPSR LA+ TCTIRL+EIFGKA R ILG+FLM GLCNGVH+LLRP
Sbjct: 29  KGRRRRNAMARTRDREPSRSLANATCTIRLTEIFGKATRSILGFFLMVGLCNGVHYLLRP 88

Query: 63  FSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAP 122
           FSQPRI SDTIVGLV+GNFIRKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYVLFKAP
Sbjct: 89  FSQPRITSDTIVGLVIGNFIRKQLDISTIKTLRYIVDFGMICYMFVLGLEVDPYVLFKAP 148

Query: 123 ARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKI 182
            RDAKVAYAGMIST ILA SI PFISF+K +EISFI+SLST LSSTASPVLTRLITSLKI
Sbjct: 149 TRDAKVAYAGMISTLILACSIIPFISFAKSKEISFIISLSTVLSSTASPVLTRLITSLKI 208

Query: 183 GKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKV 242
           GKSDIGRLVI AGMHSDFISTLMIC+GYLFC CQETRIS IKG +L ++LLIQ VLTAKV
Sbjct: 209 GKSDIGRLVIGAGMHSDFISTLMICIGYLFCKCQETRISLIKGFQLGILLLIQAVLTAKV 268

Query: 243 SPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGR 302
           SPVFMNWVNNENPEGKPMKGPHLVLA+AFMA +CCCPT FGYNPILSA+LAG FLPREGR
Sbjct: 269 SPVFMNWVNNENPEGKPMKGPHLVLAVAFMAFLCCCPTAFGYNPILSAFLAGTFLPREGR 328

Query: 303 VSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGT 362
           VSRWAIGKINYLL T+F+PIFF WMGVESKLSDFEPGQIMTWVRLI+LFAIATVGKVVGT
Sbjct: 329 VSRWAIGKINYLLTTIFFPIFFVWMGVESKLSDFEPGQIMTWVRLILLFAIATVGKVVGT 388

Query: 363 IIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHA 422
           +IAGAILGFHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFTVVHA
Sbjct: 389 VIAGAILGFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSLVMVIVIFFTVVHA 448

Query: 423 PQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPG 482
           P+VVAHII+RARKRTPTHRMALQLLDP+SELKILLC+HGPQN+AAAINIMEISRG ANPG
Sbjct: 449 PKVVAHIIQRARKRTPTHRMALQLLDPSSELKILLCVHGPQNSAAAINIMEISRGTANPG 508

Query: 483 VVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFR 542
           VVVYVTDMIELTDEIA+TLVQGEGVD+VTVTHTGVTQMRDQVT+AVQSYVDENGEGIT R
Sbjct: 509 VVVYVTDMIELTDEIASTLVQGEGVDTVTVTHTGVTQMRDQVTSAVQSYVDENGEGITLR 568

Query: 543 RMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAP 602
           RMLALST NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG S FR+VNRKVLR+AP
Sbjct: 569 RMLALSTFNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQSAFRYVNRKVLRHAP 628

Query: 603 CSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFL 662
           CSVGILVDRGLG+VEK+SRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFL
Sbjct: 629 CSVGILVDRGLGSVEKVSRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFL 688

Query: 663 VDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETY 722
           VDADAENAARRAGTYRIS AEQEEEMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETY
Sbjct: 689 VDADAENAARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETY 748

Query: 723 STLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQ 782
           STLKSL+GQYAL+IVGRGG VNSVLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII 
Sbjct: 749 STLKSLEGQYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIH 808

Query: 783 QHNLKGELDGLDDDFSIM 800
           QHNLKGELDGLDDDFSIM
Sbjct: 809 QHNLKGELDGLDDDFSIM 826

BLAST of MC09g0468 vs. NCBI nr
Match: XP_031745292.1 (cation/H(+) antiporter 28 isoform X1 [Cucumis sativus])

HSP 1 Score: 1402 bits (3628), Expect = 0.0
Identity = 712/796 (89.45%), Postives = 755/796 (94.85%), Query Frame = 0

Query: 5   RRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFS 64
           RRRR+AMA +R  EPSR LA+ TCTIRL+EIFGKA R ILG+F+M GLCNGVH+LLRPFS
Sbjct: 32  RRRRNAMAKTRNHEPSRSLANDTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFS 91

Query: 65  QPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPAR 124
           QPRI SDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYV+FKAP R
Sbjct: 92  QPRITSDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTR 151

Query: 125 DAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGK 184
           DAKVAYAGMIST ILA SI PFI   K +EISFILSLST LSSTASPVLTRLITSLKIGK
Sbjct: 152 DAKVAYAGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGK 211

Query: 185 SDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSP 244
           SDIGRLVI+AGMHSDFISTLMICVGYLFC CQETRISFIKG +L ++LLIQ VLTAKVSP
Sbjct: 212 SDIGRLVIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSP 271

Query: 245 VFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVS 304
           VFMNWVNNENPEGKPMKGPHLVLA+AFMA +CCCPT+FGYNPILSA+LAG FLPREGRVS
Sbjct: 272 VFMNWVNNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVS 331

Query: 305 RWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTII 364
           RWAIGKINYLL TVFYPIFFFWMGVESKL+DFEPGQIMTWVRLI+LFAIAT+GKVVGT+I
Sbjct: 332 RWAIGKINYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVI 391

Query: 365 AGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQ 424
           AGAILGFHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFT+VHAP+
Sbjct: 392 AGAILGFHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPK 451

Query: 425 VVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVV 484
           VVAHII+RARKRTPTHRMALQLLDP+SELKILLCIHGPQNT AAINIMEISRG ANPGVV
Sbjct: 452 VVAHIIQRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVV 511

Query: 485 VYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRM 544
           VYVTDMIELTDEIA+TLVQGEGVDSVTVTHTGVTQMR+QVT+AVQSYVDENGEGIT RRM
Sbjct: 512 VYVTDMIELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRM 571

Query: 545 LALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCS 604
           LALST NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG + FR+VNRKVLR+APCS
Sbjct: 572 LALSTFNSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCS 631

Query: 605 VGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVD 664
           VGILVDRGLG+VEKISRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVD
Sbjct: 632 VGILVDRGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVD 691

Query: 665 ADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYST 724
           ADA NAARRAGTYRIS AEQEEEMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYST
Sbjct: 692 ADAVNAARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYST 751

Query: 725 LKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQH 784
           LKSL+GQYAL+IVGRGG VN+VLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QH
Sbjct: 752 LKSLEGQYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQH 811

Query: 785 NLKGELDGLDDDFSIM 800
           NLKGELDGLDDDFSIM
Sbjct: 812 NLKGELDGLDDDFSIM 827

BLAST of MC09g0468 vs. NCBI nr
Match: XP_038896872.1 (cation/H(+) antiporter 28 isoform X1 [Benincasa hispida])

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 709/790 (89.75%), Postives = 751/790 (95.06%), Query Frame = 0

Query: 11  MAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIAS 70
           MA +R REPSR LA+ TCTIRL+EIFGKA R ILG+FLM GLCNGVH+LLRPFSQPRI S
Sbjct: 1   MARTRDREPSRSLANATCTIRLTEIFGKATRSILGFFLMVGLCNGVHYLLRPFSQPRITS 60

Query: 71  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAY 130
           DTIVGLV+GNFIRKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYVLFKAP RDAKVAY
Sbjct: 61  DTIVGLVIGNFIRKQLDISTIKTLRYIVDFGMICYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 131 AGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRL 190
           AGMIST ILA SI PFISF+K +EISFI+SLST LSSTASPVLTRLITSLKIGKSDIGRL
Sbjct: 121 AGMISTLILACSIIPFISFAKSKEISFIISLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 191 VISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWV 250
           VI AGMHSDFISTLMIC+GYLFC CQETRIS IKG +L ++LLIQ VLTAKVSPVFMNWV
Sbjct: 181 VIGAGMHSDFISTLMICIGYLFCKCQETRISLIKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 251 NNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGK 310
           NNENPEGKPMKGPHLVLA+AFMA +CCCPT FGYNPILSA+LAG FLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTAFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 311 INYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILG 370
           INYLL T+F+PIFF WMGVESKLSDFEPGQIMTWVRLI+LFAIATVGKVVGT+IAGAILG
Sbjct: 301 INYLLTTIFFPIFFVWMGVESKLSDFEPGQIMTWVRLILLFAIATVGKVVGTVIAGAILG 360

Query: 371 FHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHII 430
           FHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFTVVHAP+VVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSLVMVIVIFFTVVHAPKVVAHII 420

Query: 431 KRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDM 490
           +RARKRTPTHRMALQLLDP+SELKILLC+HGPQN+AAAINIMEISRG ANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCVHGPQNSAAAINIMEISRGTANPGVVVYVTDM 480

Query: 491 IELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTL 550
           IELTDEIA+TLVQGEGVD+VTVTHTGVTQMRDQVT+AVQSYVDENGEGIT RRMLALST 
Sbjct: 481 IELTDEIASTLVQGEGVDTVTVTHTGVTQMRDQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 551 NSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVD 610
           NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG S FR+VNRKVLR+APCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQSAFRYVNRKVLRHAPCSVGILVD 600

Query: 611 RGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENA 670
           RGLG+VEK+SRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKVSRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 671 ARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDG 730
           ARRAGTYRIS AEQEEEMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYSTLKSL+G
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 731 QYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGEL 790
           QYAL+IVGRGG VNSVLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 791 DGLDDDFSIM 800
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of MC09g0468 vs. NCBI nr
Match: XP_004136122.1 (cation/H(+) antiporter 28 isoform X2 [Cucumis sativus] >XP_031745293.1 cation/H(+) antiporter 28 isoform X2 [Cucumis sativus] >KGN45116.1 hypothetical protein Csa_016064 [Cucumis sativus])

HSP 1 Score: 1394 bits (3607), Expect = 0.0
Identity = 707/790 (89.49%), Postives = 749/790 (94.81%), Query Frame = 0

Query: 11  MAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIAS 70
           MA +R  EPSR LA+ TCTIRL+EIFGKA R ILG+F+M GLCNGVH+LLRPFSQPRI S
Sbjct: 1   MAKTRNHEPSRSLANDTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFSQPRITS 60

Query: 71  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAY 130
           DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYV+FKAP RDAKVAY
Sbjct: 61  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTRDAKVAY 120

Query: 131 AGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRL 190
           AGMIST ILA SI PFI   K +EISFILSLST LSSTASPVLTRLITSLKIGKSDIGRL
Sbjct: 121 AGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 191 VISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWV 250
           VI+AGMHSDFISTLMICVGYLFC CQETRISFIKG +L ++LLIQ VLTAKVSPVFMNWV
Sbjct: 181 VIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 251 NNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGK 310
           NNENPEGKPMKGPHLVLA+AFMA +CCCPT+FGYNPILSA+LAG FLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 311 INYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILG 370
           INYLL TVFYPIFFFWMGVESKL+DFEPGQIMTWVRLI+LFAIAT+GKVVGT+IAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVIAGAILG 360

Query: 371 FHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHII 430
           FHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFT+VHAP+VVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPKVVAHII 420

Query: 431 KRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDM 490
           +RARKRTPTHRMALQLLDP+SELKILLCIHGPQNT AAINIMEISRG ANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 491 IELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTL 550
           IELTDEIA+TLVQGEGVDSVTVTHTGVTQMR+QVT+AVQSYVDENGEGIT RRMLALST 
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 551 NSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVD 610
           NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG + FR+VNRKVLR+APCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCSVGILVD 600

Query: 611 RGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENA 670
           RGLG+VEKISRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVDADA NA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAVNA 660

Query: 671 ARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDG 730
           ARRAGTYRIS AEQEEEMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYSTLKSL+G
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 731 QYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGEL 790
           QYAL+IVGRGG VN+VLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 791 DGLDDDFSIM 800
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of MC09g0468 vs. ExPASy TrEMBL
Match: A0A6J1D1A8 (cation/H(+) antiporter 28 OS=Momordica charantia OX=3673 GN=LOC111016690 PE=4 SV=1)

HSP 1 Score: 1552 bits (4019), Expect = 0.0
Identity = 800/800 (100.00%), Postives = 800/800 (100.00%), Query Frame = 0

Query: 1   MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLL 60
           MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLL
Sbjct: 1   MPQGRRRRSAMAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLL 60

Query: 61  RPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFK 120
           RPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFK
Sbjct: 61  RPFSQPRIASDTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFK 120

Query: 121 APARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSL 180
           APARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSL
Sbjct: 121 APARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSL 180

Query: 181 KIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTA 240
           KIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTA
Sbjct: 181 KIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTA 240

Query: 241 KVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPRE 300
           KVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPRE
Sbjct: 241 KVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPRE 300

Query: 301 GRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVV 360
           GRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVV
Sbjct: 301 GRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVV 360

Query: 361 GTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVV 420
           GTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVV
Sbjct: 361 GTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVV 420

Query: 421 HAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAAN 480
           HAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAAN
Sbjct: 421 HAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAAN 480

Query: 481 PGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGIT 540
           PGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGIT
Sbjct: 481 PGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGIT 540

Query: 541 FRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRN 600
           FRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRN
Sbjct: 541 FRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRN 600

Query: 601 APCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIR 660
           APCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIR
Sbjct: 601 APCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIR 660

Query: 661 FLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSE 720
           FLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSE
Sbjct: 661 FLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSE 720

Query: 721 TYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLI 780
           TYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLI
Sbjct: 721 TYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLI 780

Query: 781 IQQHNLKGELDGLDDDFSIM 800
           IQQHNLKGELDGLDDDFSIM
Sbjct: 781 IQQHNLKGELDGLDDDFSIM 800

BLAST of MC09g0468 vs. ExPASy TrEMBL
Match: A0A0A0KBF5 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G426570 PE=4 SV=1)

HSP 1 Score: 1394 bits (3607), Expect = 0.0
Identity = 707/790 (89.49%), Postives = 749/790 (94.81%), Query Frame = 0

Query: 11  MAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIAS 70
           MA +R  EPSR LA+ TCTIRL+EIFGKA R ILG+F+M GLCNGVH+LLRPFSQPRI S
Sbjct: 1   MAKTRNHEPSRSLANDTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFSQPRITS 60

Query: 71  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAY 130
           DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYV+FKAP RDAKVAY
Sbjct: 61  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTRDAKVAY 120

Query: 131 AGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRL 190
           AGMIST ILA SI PFI   K +EISFILSLST LSSTASPVLTRLITSLKIGKSDIGRL
Sbjct: 121 AGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 191 VISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWV 250
           VI+AGMHSDFISTLMICVGYLFC CQETRISFIKG +L ++LLIQ VLTAKVSPVFMNWV
Sbjct: 181 VIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 251 NNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGK 310
           NNENPEGKPMKGPHLVLA+AFMA +CCCPT+FGYNPILSA+LAG FLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 311 INYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILG 370
           INYLL TVFYPIFFFWMGVESKL+DFEPGQIMTWVRLI+LFAIAT+GKVVGT+IAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVIAGAILG 360

Query: 371 FHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHII 430
           FHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFT+VHAP+VVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPKVVAHII 420

Query: 431 KRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDM 490
           +RARKRTPTHRMALQLLDP+SELKILLCIHGPQNT AAINIMEISRG ANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 491 IELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTL 550
           IELTDEIA+TLVQGEGVDSVTVTHTGVTQMR+QVT+AVQSYVDENGEGIT RRMLALST 
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 551 NSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVD 610
           NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG + FR+VNRKVLR+APCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCSVGILVD 600

Query: 611 RGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENA 670
           RGLG+VEKISRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVDADA NA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAVNA 660

Query: 671 ARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDG 730
           ARRAGTYRIS AEQEEEMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYSTLKSL+G
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 731 QYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGEL 790
           QYAL+IVGRGG VN+VLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 791 DGLDDDFSIM 800
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of MC09g0468 vs. ExPASy TrEMBL
Match: A0A5D3CFY8 (Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00710 PE=4 SV=1)

HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 702/790 (88.86%), Postives = 747/790 (94.56%), Query Frame = 0

Query: 11  MAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIAS 70
           MA +RV +PSR LA  +CTIRL+EIFG+A R ILG+FLM  LCNGVH+LLRPFSQPRI S
Sbjct: 1   MAKTRVHQPSRSLAIDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRITS 60

Query: 71  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAY 130
           DTIVGL VGNF+RKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYVLFKAP RDAKVAY
Sbjct: 61  DTIVGLAVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 131 AGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRL 190
           AGMIST ILA SI PFISF+K +EISFILSLST LSSTASPVLTRLITSLKIGKSDIGRL
Sbjct: 121 AGMISTLILACSIIPFISFAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 191 VISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWV 250
           VI+AGMHSDFISTL+ICVGYLFC CQETRIS +KG +L ++LLIQ VLTAKVSPVFMNWV
Sbjct: 181 VIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 251 NNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGK 310
           NNENPEGKPMKGPHLVLA+AFMA +CCCPT+FGYNPILSA+LAG FLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 311 INYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILG 370
           INYLL TVFYPIFFFWMGVESKL+DFEPGQIMTWV LI+LFAIAT+GKVVGT+IAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVTLILLFAIATIGKVVGTVIAGAILG 360

Query: 371 FHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHII 430
           FHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFTVVHAP+VV  II
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVERII 420

Query: 431 KRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDM 490
           +RARKRTPTHRMALQLLDP+SELKILLCIHGPQNT AAINIMEISRG ANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 491 IELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTL 550
           IELTDEIA+TLVQGEGVDSVTVTHTGVTQMR+QVT+AVQSYVDENGEGIT RRMLALST 
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 551 NSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVD 610
           NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG + FR+VNRKVLR+APCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILVD 600

Query: 611 RGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENA 670
           RGLG+VEKISRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 671 ARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDG 730
           ARRAGTYRIS AEQE+EMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYSTLKSL+G
Sbjct: 661 ARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 731 QYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGEL 790
           QYAL+IVGRGG VNSVLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 791 DGLDDDFSIM 800
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of MC09g0468 vs. ExPASy TrEMBL
Match: A0A1S3CE27 (cation/H(+) antiporter 28 OS=Cucumis melo OX=3656 GN=LOC103499939 PE=4 SV=1)

HSP 1 Score: 1383 bits (3579), Expect = 0.0
Identity = 701/790 (88.73%), Postives = 749/790 (94.81%), Query Frame = 0

Query: 11  MAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIAS 70
           MA +RV +PSR LA  +CTIRL+EIFG+A R ILG+FLM  LCNGVH+LLRPFSQPRI S
Sbjct: 1   MAKTRVHQPSRSLAIDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRITS 60

Query: 71  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAY 130
           DTIVGLVVGNF+RKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYVLFKAP RDAKVAY
Sbjct: 61  DTIVGLVVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 131 AGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRL 190
           AG+IST ILA SI PFIS++K +EISFILSLST LSSTASPVLTRLITSLKIGKSDIGRL
Sbjct: 121 AGLISTLILACSIIPFISYAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 191 VISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWV 250
           VI+AGMHSDFISTL+ICVGYLFC CQETRIS +KG +L ++LLIQ VLTAKVSPVFMNWV
Sbjct: 181 VIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 251 NNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGK 310
           NNENPEGKPMKGPHLVLA+AFMA +CCCPT+FGYNPILSA+LAG FLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 311 INYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILG 370
           INYLL TVFYPIFFFWMGVESKL+DFEPGQIMTWVRL++LFAIAT+GKVVGT+IAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLMLLFAIATIGKVVGTVIAGAILG 360

Query: 371 FHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHII 430
           FHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFTVVHAP+VV  II
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVECII 420

Query: 431 KRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDM 490
           +RARKRTPTHRMALQLLDP+SELKILLCIHGPQNT AAINIMEISRG ANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 491 IELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTL 550
           IELTDEIA+TLVQGEGVDSVTVTHTGVTQMR+QVT+AVQSYVDENGEGIT RRMLALST 
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 551 NSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVD 610
           NSMAQDICILAEELMGALIILPFHKSQRG+G+L EG + FR+VNRKVLR+APCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILVD 600

Query: 611 RGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENA 670
           RGLG+VEKISRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 671 ARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDG 730
           ARRAGTYRIS AEQE+EMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYSTLKSL+G
Sbjct: 661 ARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 731 QYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGEL 790
           QYAL+IVGRGG VNSVLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 791 DGLDDDFSIM 800
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of MC09g0468 vs. ExPASy TrEMBL
Match: A0A5A7UWQ7 (Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00850 PE=4 SV=1)

HSP 1 Score: 1380 bits (3573), Expect = 0.0
Identity = 700/790 (88.61%), Postives = 746/790 (94.43%), Query Frame = 0

Query: 11  MAGSRVREPSRLLADHTCTIRLSEIFGKAARYILGYFLMAGLCNGVHFLLRPFSQPRIAS 70
           MA +RV +PSR LA  +CTIRL+EIFG+A R ILG+FLM  LCNGVH+LLRPFSQPRI S
Sbjct: 1   MAKTRVHQPSRSLAIDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRITS 60

Query: 71  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAY 130
           DTIVGL VGNF+RKQLDISTIKTLRYIVDFGM CYMFVLGLE+DPYVLFKAP RDAKVAY
Sbjct: 61  DTIVGLAVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 131 AGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRL 190
           AGMIST ILA SI PFISF+K +EISFILSLST LSSTASPVLTRLITSLKIGKSDIGRL
Sbjct: 121 AGMISTLILACSIIPFISFAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 191 VISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLRLAVVLLIQTVLTAKVSPVFMNWV 250
           VI+AGMHSDFISTL+ICVGYLFC CQETRIS +KG +L ++LLIQ VLTAKVSPVFMNWV
Sbjct: 181 VIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 251 NNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGK 310
           NNENPEGKPMKGPHLVLA+AFMA +CCCPT+FGYNPILSA+LAG FLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 311 INYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILG 370
           INYLL TVFYPIFFFWMGVESKL+DFEPGQIMTWV LI+LFAIAT+GKVVGT+IAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVTLILLFAIATIGKVVGTVIAGAILG 360

Query: 371 FHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHII 430
           FHWPESVALGLLLTMKGHFHIY AIAAKTAGK TTSTS VMVIV+FFTVVHAP+VV  II
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVERII 420

Query: 431 KRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRGAANPGVVVYVTDM 490
           +RARKRTPTHRMALQLLDP+SELKILLCIHGPQNT AAINIMEISRG ANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 491 IELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTL 550
           IELTDEIA+TLVQGEGVDSVTVTHTG+TQMR+QVT+AVQSYVDENGEGIT RRMLALST 
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGLTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 551 NSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVD 610
           NSMAQDICILAEELMGALIILPFHKSQ G+G+L EG + FR+VNRKVLR+APCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQHGDGSLSEGQAAFRYVNRKVLRHAPCSVGILVD 600

Query: 611 RGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDADAENA 670
           RGLG+VEKISRSYVSQNVAVIFIGGKDDREAL YAGRVA+HPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 671 ARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDG 730
           ARRAGTYRIS AEQE+EMRQDDECFAYFYER+VAGGHVAYVEKHLASSSETYSTLKSL+G
Sbjct: 661 ARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 731 QYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKGEL 790
           QYAL+IVGRGG VNSVLTFGMNDWQQCPELGPIGD+LSGSEF+VRTSVLII QHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 791 DGLDDDFSIM 800
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of MC09g0468 vs. TAIR 10
Match: AT3G52080.1 (cation/hydrogen exchanger 28 )

HSP 1 Score: 806.2 bits (2081), Expect = 2.3e-233
Identity = 415/762 (54.46%), Postives = 544/762 (71.39%), Query Frame = 0

Query: 56  VHFLLRPFSQPRIASDTIVGLVVGNFIRKQLDIS--TIKTLRYIVDFGMTCYMFVLGLEM 115
           +H+L++P  QP + +D  +GL++GN  R +   S     TL  I++FGM C+MFV+GLEM
Sbjct: 40  LHYLMKPLGQPYLTTDFAIGLILGNIPRFRGAFSGPYSITLNNIIEFGMICHMFVMGLEM 99

Query: 116 DPYVLFKAPARDAKVAYAGMISTFILAGSITPFISFSKDREISFILSLSTALSSTASPVL 175
           +P VL + P +DA +AY  MI+TF+LA   TPF+ ++K     F L+LS   SST SP+L
Sbjct: 100 NPSVLLRPPTKDAFIAYTSMITTFVLAFVTTPFLHYTKTSPYIFSLALSLMASSTGSPIL 159

Query: 176 TRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRI----SFIKGLRLA 235
           TR+I +LKI KSD+G+L  +AG+H+D ISTL+ C G++F   ++        F + L + 
Sbjct: 160 TRVIANLKIRKSDLGKLASAAGVHTDMISTLLYCFGFIFFPTEKPLARPLHRFFRALLMF 219

Query: 236 VVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPT---IFGYNP 295
            + L Q   T+ VSP+F+NWVNNENPEGKP+KG HLV+++AF+  IC  PT      YNP
Sbjct: 220 CLFLAQVTFTSIVSPIFLNWVNNENPEGKPLKGSHLVMSLAFVVLICSFPTWPPESMYNP 279

Query: 296 ILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVR 355
           ILSA+ AG+FLP +GR+S+W I KINYLL TVFYPIFFFW+G    + +F+    M WVR
Sbjct: 280 ILSAFTAGLFLPNKGRMSKWIINKINYLLSTVFYPIFFFWVGFIIHMRNFDITDKMAWVR 339

Query: 356 LIVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTS 415
              L      GKV GT++ G +LG+H PE+ +LGLLLT KGHFH+Y A  A    +  ++
Sbjct: 340 FFSLLGTVIAGKVTGTVLCGLLLGYHVPETASLGLLLTTKGHFHVYLAALAIRTNRVKST 399

Query: 416 TSTVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTA 475
           T  +++ ++ FTVV++P VV  IIKRARKR P H MALQ LDP +EL+IL+ +HGP N  
Sbjct: 400 TGALIIFIIVFTVVYSPFVVMDIIKRARKRVPVHIMALQWLDPTTELRILIGLHGPHNIG 459

Query: 476 AAINIMEISRGAANPGVVVYVTDMIELTDEIAATLVQGEGV----DSVTVTHTGVTQMRD 535
           + +N+MEI  G   PG + Y TDM+ELTDEIAATL +G G     DSVTVT   VT+MR+
Sbjct: 460 STLNVMEICHGGREPGSIFYATDMVELTDEIAATLKKGGGAGQSNDSVTVTDRSVTEMRE 519

Query: 536 QVTAAVQSYVD-ENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNG 595
            +TAAV  Y +  NG+G+T RRMLALST  +MA D+C LA+ELM ++IILPFHK    +G
Sbjct: 520 SITAAVNGYGELRNGQGVTVRRMLALSTFVTMAHDVCGLADELMVSIIILPFHKRLNPDG 579

Query: 596 ALGEGHSGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREA 655
            L  GH+GFR VNRK+L+NAPCSVGILVDR  G  E+  R   S  +A+IFIGG+DDREA
Sbjct: 580 TLDAGHAGFRHVNRKILKNAPCSVGILVDRSFGQTEEAWRPGASMGIAIIFIGGRDDREA 639

Query: 656 LYYAGRVAKHPGVKLSVIRFLVDADAENAARRAGTY-RISEAEQEEEMRQDDECFAYFYE 715
           L +A +VA+HP VKL VIRFL D  ++NA +R+    R S  +QEEEM+ DDECFA FYE
Sbjct: 640 LAFAAQVARHPAVKLKVIRFLEDKSSQNAQKRSSILNRASVVDQEEEMKLDDECFAEFYE 699

Query: 716 RYVA-GGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGG-VNSVLTFGMNDWQQCP 775
           RY+A GG V+Y+EKHL +SSET++ LKSLDG+Y LVIVGRGGG  +S LT G+NDWQQCP
Sbjct: 700 RYIAGGGRVSYMEKHLTNSSETFTALKSLDGEYGLVIVGRGGGRASSGLTTGLNDWQQCP 759

Query: 776 ELGPIGDVLSGSEFAVRTSVLIIQQHNLKGELDGLDDDFSIM 801
           ELGPIGDVLSGS+F+  TS+LIIQQ   +G+L+GL DDF+I+
Sbjct: 760 ELGPIGDVLSGSDFSHNTSMLIIQQQRTRGQLEGLHDDFTIL 801

BLAST of MC09g0468 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 372.9 bits (956), Expect = 6.6e-103
Identity = 226/741 (30.50%), Postives = 391/741 (52.77%), Query Frame = 0

Query: 58  FLLRPFSQPRIASDTIVGLVVG--------NFIRKQLDISTIKTLRYIVDFGMTCYMFVL 117
           F+L+PF QPR+ S+ + G+V+G         F        ++  L  + + G+  ++F++
Sbjct: 56  FILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLV 115

Query: 118 GLEMDPYVLFKAPARDAKVAYAGMISTFILAGSITPFISFSKDR--EISFILSLSTALSS 177
           G+EMD  V+ K   R   +A  GM+  F++  + +  +  S+D   + ++IL L  ALS 
Sbjct: 116 GVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSV 175

Query: 178 TASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKGLR 237
           TA PVL R++  LK+  ++IGR+ +SA + +D  + +++ +     +  +T  + +  + 
Sbjct: 176 TAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMI 235

Query: 238 LAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYNPI 297
            + V +   V   +     + W+  + PEG+     H+ L +  +          G + +
Sbjct: 236 SSAVFIAVCVFVVRPG---IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSV 295

Query: 298 LSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWVRL 357
             A++ G+ +P  G +    I K+   +  +  P+FF   G+++ ++  +     TW+ L
Sbjct: 296 FGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGP--ATWLTL 355

Query: 358 IVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTTST 417
            ++  +A  GKV+GT+I     G    E + LGLLL  KG   +      K        T
Sbjct: 356 FLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDET 415

Query: 418 STVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNTAA 477
              MV+V          +V  + K  +K     R  +Q   P SEL++L+C+H P+N   
Sbjct: 416 FATMVLVALVMTGVITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPT 475

Query: 478 AINIMEISRGAANPGVVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQVTAA 537
            IN++E S       + +YV  ++ELT   +A L+      S            D +  A
Sbjct: 476 IINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINA 535

Query: 538 VQSYVDENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGH 597
            ++Y +++   +  + + A+S  ++M +D+C LAE+   + II+PFHK Q  +G +   +
Sbjct: 536 FENY-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTN 595

Query: 598 SGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGR 657
             +R VN+ +L N+PCSVGILVDRGL    +++ + VS  VAV+F GG DDREAL YA R
Sbjct: 596 PAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWR 655

Query: 658 VAKHPGVKLSVIRFLVDAD-AENAARRA---GTYRISEAEQEEEMRQDDECFAYFYERYV 717
           +A+HPG+ L+V+RF+ D D A+ A+ RA      +I + +  ++ + DD+    F     
Sbjct: 656 MAQHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENA 715

Query: 718 AGGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPI 777
               + Y+EK +++  ET + ++S+D  + L IVGRG G++S LT G+ DW +CPELG I
Sbjct: 716 EYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAI 775

Query: 778 GDVLSGSEFAVRTSVLIIQQH 785
           GD+L+ S+FA   SVL++QQ+
Sbjct: 776 GDLLASSDFAATVSVLVVQQY 789

BLAST of MC09g0468 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 309.3 bits (791), Expect = 9.0e-84
Identity = 220/745 (29.53%), Postives = 379/745 (50.87%), Query Frame = 0

Query: 58  FLLRPFSQPRIASDTIVGLVVGNFI--RKQLDISTI------KTLRYIVDFGMTCYMFVL 117
           + L+P  QPR+ ++ I G+++G     R +  + TI        L  + + G+  ++F++
Sbjct: 51  YFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVLDTLANIGLLFFLFLV 110

Query: 118 GLEMDPYVLFKAPARDAKVAYAGMISTFILAGSITPFI---SFSKD-REISFILSLSTAL 177
           GLE+D   + K   +   +A AG+   FI+ G  T F+   + SK   ++ FI+ +  AL
Sbjct: 111 GLELDFAAIKKTGKKSLLIAIAGISLPFIV-GVGTSFVLSATISKGVDQLPFIVFMGVAL 170

Query: 178 SSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMICVGYLFCDCQETRISFIKG 237
           S TA PVL R++  LK+  +DIGR+ +SA   +D  + +++ +         + +  +  
Sbjct: 171 SITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIALSGDGTSPLVSVWV 230

Query: 238 LRLAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKGPHLVLAIAFMASICCCPTIFGYN 297
           L      +I  V+  K     + ++    PEG+P+K  ++ + +  + +        G +
Sbjct: 231 LLCGTGFVIFAVVAIK---PLLAYMARRCPEGEPVKELYVCVTLTVVLAASFVTDTIGIH 290

Query: 298 PILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPIFFFWMGVESKLSDFEPGQIMTWV 357
            +  A++ GI  P+EG   R    KI  L+  +  P++F   G+++ ++     Q  +W 
Sbjct: 291 ALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLKTDVTTIRGAQ--SWG 350

Query: 358 RLIVLFAIATVGKVVGTIIAGAILGFHWPESVALGLLLTMKGHFHIYFAIAAKTAGKTTT 417
            L+++      GK+VGT+ +  +    + E+V LG L+  KG   +      K       
Sbjct: 351 LLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVELIVLNIGKDRKVLND 410

Query: 418 STSTVMVIVVFFTVVHAPQVVAHIIKRARKRTPTHRMALQLLDPASELKILLCIHGPQNT 477
               ++V++  FT      +V  I K ARK  P     +Q  D  SEL+IL C H  +N 
Sbjct: 411 QAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHRTIQRKDHDSELRILACFHSTRNI 470

Query: 478 AAAINIMEISRGAANPG-VVVYVTDMIELTDEIAATLVQGEGVDSVTVTHTGVTQMRDQV 537
              IN++E SRG    G + VY   ++EL++  +A  +  +  ++       + +  DQ+
Sbjct: 471 PTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARNNGLPIWNKIERSTDQM 530

Query: 538 TAAVQSYVDENGEGITFRRMLALSTLNSMAQDICILAEELMGALIILPFHKSQRGNGALG 597
             A ++Y  ++   +  R M A+S L+S+ +DIC  A +   A+I+LPFHK QR +GA+ 
Sbjct: 531 VIAFEAY--QHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMILLPFHKHQRMDGAME 590

Query: 598 EGHSGFRFVNRKVLRNAPCSVGILVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYY 657
                F  VN++VL+ APCSVGILVDRGLG   ++  S V+  V + F GG DDREAL Y
Sbjct: 591 SIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVVIPFFGGLDDREALAY 650

Query: 658 AGRVAKHPGVKLSVIRFLVDADAENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYV- 717
             ++ +HPG+ L+V +F+        A R    R  ++E +E+ +++ E    F    + 
Sbjct: 651 GMKMVEHPGITLTVYKFV--------AARGTLKRFEKSEHDEKEKKEKETDEEFVRELMN 710

Query: 718 ---AGGHVAYVEKHLASSSETYSTLKSLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPEL 777
                  +AY E+ + S  +  +TLKS+  +  L +VGR   V S++         CPEL
Sbjct: 711 DPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVVGRNAAVASLVKS-----TDCPEL 770

Query: 778 GPIGDVLSGSEFAVRTSVLIIQQHN 786
           GP+G +LS SEF+   SVL++Q ++
Sbjct: 771 GPVGRLLSSSEFSTTASVLVVQGYD 773

BLAST of MC09g0468 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 305.4 bits (781), Expect = 1.3e-82
Identity = 221/777 (28.44%), Postives = 387/777 (49.81%), Query Frame = 0

Query: 43  ILGYFLMAGLCNGVHFLLRPFSQPRIASDTIVGLVVG--------NFIRKQLDISTIKTL 102
           IL   ++  L   + +LLRP  QPR+ ++ I G+++G         F+       ++  L
Sbjct: 35  ILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVL 94

Query: 103 RYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAYAGMI--------STFILAGSITPF 162
             + + G+  ++F+ GLE+D   L +   +   +A AG+         S+F+L  +I+  
Sbjct: 95  ETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISKG 154

Query: 163 ISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFISTLMI 222
           ++       +F++ +  ALS TA PVL R++  LK+  ++IGRL +SA   +D  + +++
Sbjct: 155 VN-----STAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILL 214

Query: 223 CVGYLFCDCQETRI----SFIKGLRLAVVLLIQTVLTAKVSPVFMNWVNNENPEGKPMKG 282
            +         + +     F+ G    +         + + P    W++    EG+P++ 
Sbjct: 215 ALAIALSGSNTSPLVSLWVFLSGCAFVIG-------ASFIIPPIFRWISRRCHEGEPIEE 274

Query: 283 PHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGKINYLLVTVFYPI 342
            ++   +A +          G + +  A++ G+ +P+EG  +   + K+  L+  +F P+
Sbjct: 275 TYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPL 334

Query: 343 FFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILGFHWP--ESVALG 402
           +F   G+++ ++  +  Q  +W  L+++ A A  GK++GT+  G  L F  P  E++ LG
Sbjct: 335 YFVASGLKTNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLG 394

Query: 403 LLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHIIK---RARKRT 462
            L+  KG   +      K        T  +MV++  FT      VV  + K   RA+K  
Sbjct: 395 FLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEG 454

Query: 463 PTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRG-AANPGVVVYVTDMIELTDE 522
                A++  +  ++L+IL C HG  +  + IN++E SRG     G+ VY   + EL++ 
Sbjct: 455 EYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSER 514

Query: 523 IAATL----VQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLALSTLNS 582
            +A L    V+  G+        GV    DQV  A Q++  +    +  R M A+S+++ 
Sbjct: 515 SSAILMVHKVRKNGMP--FWNRRGVNADADQVVVAFQAF--QQLSRVNVRPMTAISSMSD 574

Query: 583 MAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGILVDRG 642
           + +DIC  A     A++ILPFHK Q+ +G+L      +R+VNR+VL  APCSVGI VDRG
Sbjct: 575 IHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRG 634

Query: 643 LGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDAD-AENAA 702
           LG   ++S   VS +V V+F GG DDREAL Y  R+A+HPG+ L+V RF+V  +      
Sbjct: 635 LGGSSQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPERVGEIV 694

Query: 703 RRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLKSLDGQ 762
               +   +E +  + ++ D+E  +   +       V +VEK + +++    +      +
Sbjct: 695 NVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVRR 754

Query: 763 YALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNLKG 789
             L +VGR  G    L    N   +CPELGP+G +L   E + + SVL+IQQ+N  G
Sbjct: 755 SNLFLVGRMPGGEIALAIREN--SECPELGPVGSLLISPESSTKASVLVIQQYNGTG 789

BLAST of MC09g0468 vs. TAIR 10
Match: AT5G41610.2 (cation/H+ exchanger 18 )

HSP 1 Score: 288.9 bits (738), Expect = 1.3e-77
Identity = 207/722 (28.67%), Postives = 362/722 (50.14%), Query Frame = 0

Query: 90  TIKTLRYIVDFGMTCYMFVLGLEMDPYVLFKAPARDAKVAYAGMI--------STFILAG 149
           ++  L  + + G+  ++F+ GLE+D   L +   +   +A AG+         S+F+L  
Sbjct: 22  SLTVLETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKA 81

Query: 150 SITPFISFSKDREISFILSLSTALSSTASPVLTRLITSLKIGKSDIGRLVISAGMHSDFI 209
           +I+  ++       +F++ +  ALS TA PVL R++  LK+  ++IGRL +SA   +D  
Sbjct: 82  TISKGVN-----STAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVA 141

Query: 210 STLMICVGYLFCDCQETRI----SFIKGLRLAVVLLIQTVLTAKVSPVFMNWVNNENPEG 269
           + +++ +         + +     F+ G    +         + + P    W++    EG
Sbjct: 142 AWILLALAIALSGSNTSPLVSLWVFLSGCAFVIG-------ASFIIPPIFRWISRRCHEG 201

Query: 270 KPMKGPHLVLAIAFMASICCCPTIFGYNPILSAYLAGIFLPREGRVSRWAIGKINYLLVT 329
           +P++  ++   +A +          G + +  A++ G+ +P+EG  +   + K+  L+  
Sbjct: 202 EPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSG 261

Query: 330 VFYPIFFFWMGVESKLSDFEPGQIMTWVRLIVLFAIATVGKVVGTIIAGAILGFHWP--E 389
           +F P++F   G+++ ++  +  Q  +W  L+++ A A  GK++GT+  G  L F  P  E
Sbjct: 262 LFLPLYFVASGLKTNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMRE 321

Query: 390 SVALGLLLTMKGHFHIYFAIAAKTAGKTTTSTSTVMVIVVFFTVVHAPQVVAHIIK---R 449
           ++ LG L+  KG   +      K        T  +MV++  FT      VV  + K   R
Sbjct: 322 AITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARR 381

Query: 450 ARKRTPTHRMALQLLDPASELKILLCIHGPQNTAAAINIMEISRG-AANPGVVVYVTDMI 509
           A+K       A++  +  ++L+IL C HG  +  + IN++E SRG     G+ VY   + 
Sbjct: 382 AKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLR 441

Query: 510 ELTDEIAATL----VQGEGVDSVTVTHTGVTQMRDQVTAAVQSYVDENGEGITFRRMLAL 569
           EL++  +A L    V+  G+        GV    DQV  A Q++  +    +  R M A+
Sbjct: 442 ELSERSSAILMVHKVRKNGMP--FWNRRGVNADADQVVVAFQAF--QQLSRVNVRPMTAI 501

Query: 570 STLNSMAQDICILAEELMGALIILPFHKSQRGNGALGEGHSGFRFVNRKVLRNAPCSVGI 629
           S+++ + +DIC  A     A++ILPFHK Q+ +G+L      +R+VNR+VL  APCSVGI
Sbjct: 502 SSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGI 561

Query: 630 LVDRGLGTVEKISRSYVSQNVAVIFIGGKDDREALYYAGRVAKHPGVKLSVIRFLVDAD- 689
            VDRGLG   ++S   VS +V V+F GG DDREAL Y  R+A+HPG+ L+V RF+V  + 
Sbjct: 562 FVDRGLGGSSQVSAQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPER 621

Query: 690 AENAARRAGTYRISEAEQEEEMRQDDECFAYFYERYVAGGHVAYVEKHLASSSETYSTLK 749
                    +   +E +  + ++ D+E  +   +       V +VEK + +++    +  
Sbjct: 622 VGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAI 681

Query: 750 SLDGQYALVIVGRGGGVNSVLTFGMNDWQQCPELGPIGDVLSGSEFAVRTSVLIIQQHNL 789
               +  L +VGR  G    L    N   +CPELGP+G +L   E + + SVL+IQQ+N 
Sbjct: 682 EEVRRSNLFLVGRMPGGEIALAIREN--SECPELGPVGSLLISPESSTKASVLVIQQYNG 721

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7093.3e-23254.46Cation/H(+) antiporter 28 OS=Arabidopsis thaliana OX=3702 GN=CHX28 PE=2 SV=2[more]
Q9SIT59.3e-10230.50Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9LUN41.3e-8229.53Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1[more]
Q9FFR91.8e-8128.44Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1[more]
Q8VYD42.0e-6726.20Cation/H(+) antiporter 23, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHX2... [more]
Match NameE-valueIdentityDescription
XP_022147855.10.0100.00cation/H(+) antiporter 28 [Momordica charantia][more]
XP_038896873.10.089.60cation/H(+) antiporter 28 isoform X2 [Benincasa hispida][more]
XP_031745292.10.089.45cation/H(+) antiporter 28 isoform X1 [Cucumis sativus][more]
XP_038896872.10.089.75cation/H(+) antiporter 28 isoform X1 [Benincasa hispida][more]
XP_004136122.10.089.49cation/H(+) antiporter 28 isoform X2 [Cucumis sativus] >XP_031745293.1 cation/H(... [more]
Match NameE-valueIdentityDescription
A0A6J1D1A80.0100.00cation/H(+) antiporter 28 OS=Momordica charantia OX=3673 GN=LOC111016690 PE=4 SV... [more]
A0A0A0KBF50.089.49Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G426... [more]
A0A5D3CFY80.088.86Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CE270.088.73cation/H(+) antiporter 28 OS=Cucumis melo OX=3656 GN=LOC103499939 PE=4 SV=1[more]
A0A5A7UWQ70.088.61Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
Match NameE-valueIdentityDescription
AT3G52080.12.3e-23354.46cation/hydrogen exchanger 28 [more]
AT2G13620.16.6e-10330.50cation/hydrogen exchanger 15 [more]
AT3G17630.19.0e-8429.53cation/H+ exchanger 19 [more]
AT5G41610.11.3e-8228.44cation/H+ exchanger 18 [more]
AT5G41610.21.3e-7728.67cation/H+ exchanger 18 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 39..439
e-value: 4.1E-52
score: 179.3
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 56..429
e-value: 1.4E-29
score: 103.1
NoneNo IPR availablePANTHERPTHR32468:SF145CATION/H(+) ANTIPORTER 28coord: 32..797
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 32..797

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0468.1MC09g0468.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0015299 solute:proton antiporter activity