MC09g0400 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0400
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING/U-box superfamily protein
Location	MC09: 3610741 .. 3613415 (-)
RNA-Seq Expression	MC09g0400
Synteny	MC09g0400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGCAAAACCCGGTTTAACGCCTGAGGCATTTTGCCATATGTTTTAAATTTTTGACAAATATTAAAGATCATTAAGTGCATTTAGCCAAATTACCGCTCATTTTTTCCCCCACCCTTATTTCCGCCCAATTTGAGAAAATCAGAGTGGTTTCTTTTTTCAAAATGGCAACCGATTCTTCGTTTGCCTTCCTCTGATTTTCTCTTCTAGGCGCTAATGTGATTACACTCCCTCTGATCACTAATAGCCTCTCCGCTCCGCCGTAGACAGTAAAAACGACATGTCGTCTACCGCATCCTCCGTCGGTTTGTCGTCTACTCCGTTAACCGTACCGCCCGAAAATCCATCGCGAAAATCCAAAAATAGCAGTCGATCGTCTCGGCAACTGTGCGAGATTTGTTTAGAGAGAAAAGCAATCGACAACATGTTTACAATCGAGGCCTGTCTTCACTCCTTCTGCCGCAAATGCGTGATTAAACATGTCGCCGCGAAGATCAACGGCGGATCTTCGCGCGTTTCTTGCCCTAGCTTGGACTGCTCAAGATTCGTAGAGTTCGAGACCTGCTGGTAACGAAATCTTTCTCAACTTCCTTGTTTCTGTTTGGTTGGGAAGATTTTGAGTGGAAATCGTGATTTTAAATTCAAGAAATGAAAAGAAAATCAAGAAGTTCAATAGTGTTTTCATTCTATTGTATACTGTAGGTTGATTTTGATTTTCAATTTTGTTTAACAATTTTCTAATCCGGGAATGTGTACACATGGTAATGAGTTCGTGTTTTGTCATCTGCTAGGGTTTGGATTTGAGATAAAAATAGAGAAATGAAAGATCAAAATAGTTTTTAGATTTGAAGTTTTTAAAATGATGGTGACCAAAGTTGTAATTTAACAGAATTTGAATTCCTCTAAAAAAAATAGTAATAATAATAATAAAATTAATGTGATTCATTTAAGTGATTTCATAATCATGGAAAGTGGACGTTTTGTTTTTTCATTTCGAATTTGCAGGGGAATGCTACCGAAAAGGGTGGCGGAGAAGTGGGATAGAGCGCTTTGCGAAGCGCTGTTTGTGGAAGCAGAAAAAGTGATGTGCCCTTTCAAGGACTGCTCGGCGGCGATGGTGAATCTTGAGAAGGGGCGGTCGGCAGATATATTGGAGAGCGAGTGCCCGATTTGCCACAGGCTGTTCTGCGCCAAGTGCCGGGTCCCGTGGCACGCGAGAGTCACGTGCGAGGAATATCAGAGACAGAAAAAATCAAAGAAGAGAGGGAGAGAGGATGATGACGCGTTGTTGAAGGATATGGCCCGTAAAATGGAGTGGATGAGATGCCCTCGCTGCAAGTTCTTCGTCGAAAAGACTTATGGTTGCTTACATATCACTTGCAGGTTCCTTTCTTTCTCTGATTTTGCATTCTCATTTTCATCATGATTTTCTTCTGCTTCTTTCCCCTTTGAATCATATTTAGAAACATAGCTATTAAATATTTAAGATAGGATACTAGCCCAGCCAAACATACTCTCATGCTTGATTTAGATTATAGTTTGTTTCTAGATACTCTTCGGTTCATATTTTGGAACTTCCAAAATCGAGAGTGTTTGGCAAATTTCGGTTGGAGACCTAGACTCGGGGTAAGCCGGGCACGGTTTGAGGCTTGGCCCAGGGTTGCCCCTTGGGTCATGCCTAGCCATTTTGGATTGAGCCTGAGGCTCGGCTCATTCGAATCATCACTTAGTTTCTTCCGTCTTAGTTCCAAGTTTTCTTTGTAGGTTGGGATGAAAATCTTCAATTTTGTGTTTATTGTTCACTAAGACCTACCAAGAAAATGAAAATTTGGGACTTATTTGATTCAACTTGATAAAATCATGATTTTTTAAGGATCTGAACTTATAATTTTGTACTGAATTGATTCAAATTGATGTTGAAAAGGATTTTCGTTTTAAAAGAATATATAACGTGTGGGTAGAGGTGGATCTATAATTTATATATATATTTAAAAAAATAGAAAACCTCAATTAATAACAAAAACGAATAAACTTTGGGTCAAACCTTGATATCATCTTTGAAAGTTGAAATTCAAATCAAACAAAAGTGAAGTCGATGAGAGGTGTATTTTACCATATTTTATACATTAGGTTATGCATTGGGTCTTATGTTAAAATTTGATGTTTAGATTTCCGCATCGGGATAGATCATATAATTTATATGCTTCAACTCGATTAGTCTTTTTAATATGCAACCGAAATTTGAATGTCCATATTAAATGAATGTTTATATACCATATTTTAAGAGTTAAATATTATTATAATGTATGCTTTATTTTGTATGGTTCATGAAATTGTTGTAGGTGTAAATTCGAGTTTTGTTATGCCTGTGGATCACAGTGGTCTCACAACCATGGTGGGTGCCGCCAACCCAATTGACCCACTCAGCCAAAAAAAAATTTACACTGCACCTTTTTTTTTTTAATTTATTTTTATTCAGGATGTTTCTTTTGGCTCATATTTTCTAGAAATATGAGGTAAGAAGACAATAGGAAATATTCAAGCTTGGGTTGAATATTTGCTACACAGTTCAATTCAATTGTTTTGAAATGGTGAAGGGAATTATGATGGACAAATTTTTTGTTGTAAATGATATCTTCTCCTCTCTTTCGGTTTACCTTTTAAACATGTGGCATAT

mRNA sequence

CGGCAAAACCCGGTTTAACGCCTGAGGCATTTTGCCATATGTTTTAAATTTTTGACAAATATTAAAGATCATTAAGTGCATTTAGCCAAATTACCGCTCATTTTTTCCCCCACCCTTATTTCCGCCCAATTTGAGAAAATCAGAGTGGTTTCTTTTTTCAAAATGGCAACCGATTCTTCGTTTGCCTTCCTCTGATTTTCTCTTCTAGGCGCTAATGTGATTACACTCCCTCTGATCACTAATAGCCTCTCCGCTCCGCCGTAGACAGTAAAAACGACATGTCGTCTACCGCATCCTCCGTCGGTTTGTCGTCTACTCCGTTAACCGTACCGCCCGAAAATCCATCGCGAAAATCCAAAAATAGCAGTCGATCGTCTCGGCAACTGTGCGAGATTTGTTTAGAGAGAAAAGCAATCGACAACATGTTTACAATCGAGGCCTGTCTTCACTCCTTCTGCCGCAAATGCGTGATTAAACATGTCGCCGCGAAGATCAACGGCGGATCTTCGCGCGTTTCTTGCCCTAGCTTGGACTGCTCAAGATTCGTAGAGTTCGAGACCTGCTGGGGAATGCTACCGAAAAGGGTGGCGGAGAAGTGGGATAGAGCGCTTTGCGAAGCGCTGTTTGTGGAAGCAGAAAAAGTGATGTGCCCTTTCAAGGACTGCTCGGCGGCGATGGTGAATCTTGAGAAGGGGCGGTCGGCAGATATATTGGAGAGCGAGTGCCCGATTTGCCACAGGCTGTTCTGCGCCAAGTGCCGGGTCCCGTGGCACGCGAGAGTCACGTGCGAGGAATATCAGAGACAGAAAAAATCAAAGAAGAGAGGGAGAGAGGATGATGACGCGTTGTTGAAGGATATGGCCCGTAAAATGGAGTGGATGAGATGCCCTCGCTGCAAGTTCTTCGTCGAAAAGACTTATGGTTGCTTACATATCACTTGCAGGTGTAAATTCGAGTTTTGTTATGCCTGTGGATCACAGTGGTCTCACAACCATGGTGGGTGCCGCCAACCCAATTGACCCACTCAGCCAAAAAAAAATTTACACTGCACCTTTTTTTTTTTAATTTATTTTTATTCAGGATGTTTCTTTTGGCTCATATTTTCTAGAAATATGAGGTAAGAAGACAATAGGAAATATTCAAGCTTGGGTTGAATATTTGCTACACAGTTCAATTCAATTGTTTTGAAATGGTGAAGGGAATTATGATGGACAAATTTTTTGTTGTAAATGATATCTTCTCCTCTCTTTCGGTTTACCTTTTAAACATGTGGCATAT

Coding sequence (CDS)

ATGTCGTCTACCGCATCCTCCGTCGGTTTGTCGTCTACTCCGTTAACCGTACCGCCCGAAAATCCATCGCGAAAATCCAAAAATAGCAGTCGATCGTCTCGGCAACTGTGCGAGATTTGTTTAGAGAGAAAAGCAATCGACAACATGTTTACAATCGAGGCCTGTCTTCACTCCTTCTGCCGCAAATGCGTGATTAAACATGTCGCCGCGAAGATCAACGGCGGATCTTCGCGCGTTTCTTGCCCTAGCTTGGACTGCTCAAGATTCGTAGAGTTCGAGACCTGCTGGGGAATGCTACCGAAAAGGGTGGCGGAGAAGTGGGATAGAGCGCTTTGCGAAGCGCTGTTTGTGGAAGCAGAAAAAGTGATGTGCCCTTTCAAGGACTGCTCGGCGGCGATGGTGAATCTTGAGAAGGGGCGGTCGGCAGATATATTGGAGAGCGAGTGCCCGATTTGCCACAGGCTGTTCTGCGCCAAGTGCCGGGTCCCGTGGCACGCGAGAGTCACGTGCGAGGAATATCAGAGACAGAAAAAATCAAAGAAGAGAGGGAGAGAGGATGATGACGCGTTGTTGAAGGATATGGCCCGTAAAATGGAGTGGATGAGATGCCCTCGCTGCAAGTTCTTCGTCGAAAAGACTTATGGTTGCTTACATATCACTTGCAGGTGTAAATTCGAGTTTTGTTATGCCTGTGGATCACAGTGGTCTCACAACCATGGTGGGTGCCGCCAACCCAATTGA

Protein sequence

MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHGGCRQPN

Homology

[Close]

BLAST of MC09g0400 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 105.9 bits (263), Expect = 6.6e-22
Identity = 61/212 (28.77%), Postives = 101/212 (47.64%), Query Frame = 0


Query: 36  LCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETC 95
           +C +CLE K I     +  C  + C +C+  +++A++  G   + CP  +C  F+E  T 
Sbjct: 262 MCRVCLEDKPIK---PLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEETTV 321

Query: 96  WGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKG------RSADILESEC 155
              L    + K+ +   E   +++    CP   C       +KG      RS    + +C
Sbjct: 322 VYNLTHEDSIKY-KYFLELGRIDSSTKPCP--QCKHFTTFKKKGHIPTPSRSESKYKIQC 381

Query: 156 PICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEW-----MRCP 215
           P C  ++C KC  PWH  V C+EY++            D LL+  A ++E       +CP
Sbjct: 382 PTCQFVWCFKCHSPWHEGVNCKEYKK-----------GDKLLRHWASEIEHGQRNAQKCP 441

Query: 216 RCKFFVEKTYGCLHITC-RCKFEFCYACGSQW 236
           +CK  +++T GC H+TC +C   FCY CG ++
Sbjct: 442 KCKIHIQRTEGCDHMTCSQCNTNFCYRCGERY 456

[Close]

BLAST of MC09g0400 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.1e-21
Identity = 60/212 (28.30%), Postives = 101/212 (47.64%), Query Frame = 0


Query: 36  LCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETC 95
           +C +CLE K I     +  C  + C +C+  ++++++  G   + CP  +C  F+E  T 
Sbjct: 235 MCRVCLEDKPIK---PLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLEETTV 294

Query: 96  WGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKG------RSADILESEC 155
              L    + K+ +   E   +++    CP   C       +KG      RS    + +C
Sbjct: 295 VYNLTHEDSIKY-KYFLELGRIDSSTKPCP--QCKHFTTFKKKGHIPTPSRSESRYKIQC 354

Query: 156 PICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEW-----MRCP 215
           P C  ++C KC  PWH  V C+EY++            D LL+  A ++E       +CP
Sbjct: 355 PTCQLIWCFKCHSPWHEGVNCKEYKK-----------GDKLLRHWASEIEHGQRNAQKCP 414

Query: 216 RCKFFVEKTYGCLHITC-RCKFEFCYACGSQW 236
           +CK  +++T GC H+TC +C   FCY CG ++
Sbjct: 415 KCKIHIQRTEGCDHMTCSQCNTNFCYRCGERY 429

[Close]

BLAST of MC09g0400 vs. ExPASy Swiss-Prot
Match: Q4KLT0 (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.3e-21
Identity = 56/209 (26.79%), Postives = 101/209 (48.33%), Query Frame = 0


Query: 37  CEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCW 96
           C +CLE ++I     +  C    C +C+ +++++++  G + + CP  +C++ ++  T  
Sbjct: 3   CRVCLEDRSIK---PLPCCKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTIL 62

Query: 97  GMLPKRVAEKWDRALCEALFVEAEKVMCP----FKDCSAAMVNLEKGRSADILESECPIC 156
             LP     K+ +   E   +++    CP    F             +S + L+ +CP C
Sbjct: 63  YSLPHDDIIKY-KYFLELSRMDSSTKPCPQCKHFTTFKRKTHIPNPTKSENKLKIQCPSC 122

Query: 157 HRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEW-----MRCPRCK 216
             ++C +C  PWH  V C EY++            D LL+  A ++E       +CPRCK
Sbjct: 123 QFIWCFRCHAPWHEGVNCREYKK-----------GDKLLRHWANEIEHGQRNAQKCPRCK 182

Query: 217 FFVEKTYGCLHITC-RCKFEFCYACGSQW 236
             +++T GC H+TC +C   FCY CG ++
Sbjct: 183 VHIQRTEGCDHMTCSQCNTNFCYRCGERY 196

[Close]

BLAST of MC09g0400 vs. ExPASy Swiss-Prot
Match: P0CE10 (ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01020 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.0e-19
Identity = 58/202 (28.71%), Postives = 92/202 (45.54%), Query Frame = 0


Query: 37   CEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKI-NGGSSRVSCPSLDCSRFVEFETC 96
            C ICL    +D+ +++E C H FC+ C+++   A + N  +  + C  +DC   +     
Sbjct: 1561 CPICLSE--VDDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1620

Query: 97   WGMLPKRVAEKWDRALCEALFVEAEKVM--CPFKDCSAAMVNLEKGRSADILESE----C 156
              +L +   ++   A   A    ++  +  C   DC +        R A   ES     C
Sbjct: 1621 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIY------RVAGPQESGEPFIC 1680

Query: 157  PICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFF 216
              CH   C +C + +H  +TCE Y       K+ +E+ D  LKD A+  +   CP CK  
Sbjct: 1681 GACHSETCTRCHLEYHPLITCERY-------KKFKENPDLSLKDWAKGKDVKECPICKST 1740

Query: 217  VEKTYGCLHITCRCKFEFCYAC 232
            +EKT GC H+ CRC    C+ C
Sbjct: 1741 IEKTDGCNHLQCRCGKHICWTC 1747

[Close]

BLAST of MC09g0400 vs. ExPASy Swiss-Prot
Match: F4KGU4 (ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10370 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-18
Identity = 62/205 (30.24%), Postives = 93/205 (45.37%), Query Frame = 0


Query: 37   CEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKI-NGGSSRVSCPSLDCSRFVEFETC 96
            C ICL    +D+ +++E C H FC+ C+++   A + N  +  + C  +DC   +     
Sbjct: 1564 CPICLSE--VDDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVLADM 1623

Query: 97   WGMLPKRVAEKWDRALCEAL--FV---EAEKVMCPFKDCSAAMVNLEKGRSADILESE-- 156
              +L +   EK D     +L  FV   + +   C   DC +        R A   ES   
Sbjct: 1624 RALLSQ---EKLDELFSASLSSFVTSSDGKFRFCSTPDCPSVY------RVAGPQESGEP 1683

Query: 157  --CPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRC 216
              C  CH   C +C + +H  +TCE Y       K+ +E+ D  LKD A+      CP C
Sbjct: 1684 FICGACHSEICTRCHLEYHPLITCERY-------KKFKENPDLSLKDWAKGKNVKECPIC 1743

Query: 217  KFFVEKTYGCLHITCRCKFEFCYAC 232
            K  +EKT GC H+ CRC    C+ C
Sbjct: 1744 KSTIEKTDGCNHMKCRCGKHICWTC 1750

[Close]

BLAST of MC09g0400 vs. NCBI nr
Match: XP_022146079.1 (probable E3 ubiquitin-protein ligase RNF217 [Momordica charantia])

HSP 1 Score: 518 bits (1334), Expect = 9.76e-186
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFC 60
           MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFC
Sbjct: 1   MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFC 60

Query: 61  RKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAE 120
           RKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAE
Sbjct: 61  RKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAE 120

Query: 121 KVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSK 180
           KVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSK
Sbjct: 121 KVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSK 180

Query: 181 KRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHG 240
           KRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHG
Sbjct: 181 KRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHG 240

Query: 241 GCRQPN 246
           GCRQPN
Sbjct: 241 GCRQPN 246

[Close]

BLAST of MC09g0400 vs. NCBI nr
Match: KAA0058684.1 (putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa])

HSP 1 Score: 280 bits (717), Expect = 2.44e-92
Identity = 127/209 (60.77%), Postives = 156/209 (74.64%), Query Frame = 0


Query: 34  RQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFE 93
           ++LCEICLE K   NMF I+ CLHSFCR CVIKHV+ KI  G S VSC +L+CS  ++F 
Sbjct: 2   KKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFH 61

Query: 94  TCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICH 153
           +CW MLP   AEKW++ALCE ++ E EKV+CPFKDCS  M+     R A I + ECP+CH
Sbjct: 62  SCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIV---ERDAKIRDCECPLCH 121

Query: 154 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 213
           RLFC  CRVPWH  VTCE+Y+R  K +  GR  D+ L+K +A +M+WM+CP+C+FFVEK 
Sbjct: 122 RLFCGTCRVPWHEGVTCEDYKRLYKYED-GR--DEILMKKLASQMKWMKCPKCQFFVEKV 181

Query: 214 YGCLHITCRCKFEFCYACGSQWSHNHGGC 242
            GCLHITCRC+FEFCYACGS WS  HGGC
Sbjct: 182 SGCLHITCRCRFEFCYACGSAWSSTHGGC 204

[Close]

BLAST of MC09g0400 vs. NCBI nr
Match: TYK10490.1 (putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa])

HSP 1 Score: 279 bits (713), Expect = 9.89e-92
Identity = 126/209 (60.29%), Postives = 156/209 (74.64%), Query Frame = 0


Query: 34  RQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFE 93
           ++LCEICLE K   NMF I+ CLHSFCR CVIKHV+ KI  G S VSC +L+CS  ++F 
Sbjct: 2   KKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFH 61

Query: 94  TCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICH 153
           +CW MLP   AEKW++ALCE ++ E EKV+CPFKDCS  M+     R A I + ECP+CH
Sbjct: 62  SCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIV---ERDAKIRDCECPLCH 121

Query: 154 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 213
           RLFC  CRVPWH  VTCE+Y+R  K ++ GR  D+ L+K +A +M+WM+CP+C+ FVEK 
Sbjct: 122 RLFCGTCRVPWHEGVTCEDYKRLYKYEE-GR--DEILMKKLASQMKWMKCPKCQLFVEKV 181

Query: 214 YGCLHITCRCKFEFCYACGSQWSHNHGGC 242
            GCLHITCRC+FEFCYACGS WS  HGGC
Sbjct: 182 SGCLHITCRCRFEFCYACGSAWSSTHGGC 204

[Close]

BLAST of MC09g0400 vs. NCBI nr
Match: KAE8646419.1 (hypothetical protein Csa_016113 [Cucumis sativus])

HSP 1 Score: 268 bits (685), Expect = 1.03e-87
Identity = 122/198 (61.62%), Postives = 150/198 (75.76%), Query Frame = 0


Query: 49  MFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWD 108
           MF I  CLHSFCR CVIKHV+ KI  G S VSC +L+C   ++F +CW MLPK + EKW+
Sbjct: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60

Query: 109 RALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARV 168
           +ALCE L+   EKV+CPFKDCS   + LE+G  A I + ECP+CHRLFCA C VPWH  V
Sbjct: 61  KALCEVLYSTEEKVVCPFKDCSVG-ITLERG--ASIRDCECPLCHRLFCATCEVPWHDGV 120

Query: 169 TCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFC 228
           TCEEY+R  K+++ GR  ++ L+K +A +M+WM+CP+C FFVEK YGCLHITCRC+FEFC
Sbjct: 121 TCEEYKRLYKNEE-GR--NEILMKKLASQMKWMKCPKCNFFVEKIYGCLHITCRCRFEFC 180

Query: 229 YACGSQWSHNHGGCRQPN 246
           YACGS WS  HGGC QPN
Sbjct: 181 YACGSAWSSTHGGC-QPN 191

[Close]

BLAST of MC09g0400 vs. NCBI nr
Match: GAU14896.1 (hypothetical protein TSUD_46890 [Trifolium subterraneum])

HSP 1 Score: 263 bits (673), Expect = 1.90e-84
Identity = 117/219 (53.42%), Postives = 149/219 (68.04%), Query Frame = 0


Query: 25  KSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSL 84
           +S  SS SS  +CEIC E K  + MF  + C HSFC +CVIK V  KIN   + VSCP L
Sbjct: 75  ESNESSSSSIIICEICAETKKTEEMFNNQNCSHSFCSECVIKQVETKINEKIANVSCPGL 134

Query: 85  DCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADI 144
           +C   +E E+C  +LPK + +KW+ ALCEALF    K  CPFKDCSA +++  +G   DI
Sbjct: 135 NCKGVIELESCISLLPKELIDKWNDALCEALFCSVPKFYCPFKDCSAMLLDENEGGGEDI 194

Query: 145 LESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCP 204
            ESECP CHRLFCA C VPWH  + CE+YQ+    +   R  DD +++++A + +W RCP
Sbjct: 195 RESECPFCHRLFCASCHVPWHPGIGCEDYQKLNVDE---RGSDDLVVRELANEKKWRRCP 254

Query: 205 RCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHGGCR 243
            CKF+VEK  GCLH+TCRC+FEFCYACG QWS  HGGC+
Sbjct: 255 TCKFYVEKNGGCLHVTCRCRFEFCYACGEQWSSTHGGCQ 290

[Close]

BLAST of MC09g0400 vs. ExPASy TrEMBL
Match: A0A6J1CX43 (probable E3 ubiquitin-protein ligase RNF217 OS=Momordica charantia OX=3673 GN=LOC111015371 PE=3 SV=1)

HSP 1 Score: 518 bits (1334), Expect = 4.73e-186
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0


Query: 1   MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFC 60
           MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFC
Sbjct: 1   MSSTASSVGLSSTPLTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFC 60

Query: 61  RKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAE 120
           RKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAE
Sbjct: 61  RKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAE 120

Query: 121 KVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSK 180
           KVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSK
Sbjct: 121 KVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSK 180

Query: 181 KRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHG 240
           KRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHG
Sbjct: 181 KRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHG 240

Query: 241 GCRQPN 246
           GCRQPN
Sbjct: 241 GCRQPN 246

[Close]

BLAST of MC09g0400 vs. ExPASy TrEMBL
Match: A0A5A7UU31 (Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001540 PE=3 SV=1)

HSP 1 Score: 280 bits (717), Expect = 1.18e-92
Identity = 127/209 (60.77%), Postives = 156/209 (74.64%), Query Frame = 0


Query: 34  RQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFE 93
           ++LCEICLE K   NMF I+ CLHSFCR CVIKHV+ KI  G S VSC +L+CS  ++F 
Sbjct: 2   KKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFH 61

Query: 94  TCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICH 153
           +CW MLP   AEKW++ALCE ++ E EKV+CPFKDCS  M+     R A I + ECP+CH
Sbjct: 62  SCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIV---ERDAKIRDCECPLCH 121

Query: 154 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 213
           RLFC  CRVPWH  VTCE+Y+R  K +  GR  D+ L+K +A +M+WM+CP+C+FFVEK 
Sbjct: 122 RLFCGTCRVPWHEGVTCEDYKRLYKYED-GR--DEILMKKLASQMKWMKCPKCQFFVEKV 181

Query: 214 YGCLHITCRCKFEFCYACGSQWSHNHGGC 242
            GCLHITCRC+FEFCYACGS WS  HGGC
Sbjct: 182 SGCLHITCRCRFEFCYACGSAWSSTHGGC 204

[Close]

BLAST of MC09g0400 vs. ExPASy TrEMBL
Match: A0A5D3CGK1 (Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001400 PE=3 SV=1)

HSP 1 Score: 279 bits (713), Expect = 4.79e-92
Identity = 126/209 (60.29%), Postives = 156/209 (74.64%), Query Frame = 0


Query: 34  RQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFE 93
           ++LCEICLE K   NMF I+ CLHSFCR CVIKHV+ KI  G S VSC +L+CS  ++F 
Sbjct: 2   KKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFH 61

Query: 94  TCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICH 153
           +CW MLP   AEKW++ALCE ++ E EKV+CPFKDCS  M+     R A I + ECP+CH
Sbjct: 62  SCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIV---ERDAKIRDCECPLCH 121

Query: 154 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 213
           RLFC  CRVPWH  VTCE+Y+R  K ++ GR  D+ L+K +A +M+WM+CP+C+ FVEK 
Sbjct: 122 RLFCGTCRVPWHEGVTCEDYKRLYKYEE-GR--DEILMKKLASQMKWMKCPKCQLFVEKV 181

Query: 214 YGCLHITCRCKFEFCYACGSQWSHNHGGC 242
            GCLHITCRC+FEFCYACGS WS  HGGC
Sbjct: 182 SGCLHITCRCRFEFCYACGSAWSSTHGGC 204

[Close]

BLAST of MC09g0400 vs. ExPASy TrEMBL
Match: A0A2K3N511 (Putative E3 ubiquitin-protein ligase RNF144A-like protein OS=Trifolium pratense OX=57577 GN=L195_g021354 PE=3 SV=1)

HSP 1 Score: 261 bits (668), Expect = 5.60e-84
Identity = 119/220 (54.09%), Postives = 151/220 (68.64%), Query Frame = 0


Query: 25  KSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSL 84
           +S  SS SS  +CEIC E K  + MF  ++C HSFC +CVIK V  KIN   + VSCP L
Sbjct: 78  ESNESSSSSIIICEICAETKKTEEMFNNQSCSHSFCSECVIKQVETKINEKIANVSCPGL 137

Query: 85  DCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADI 144
           +C   +E E+C  +LPK + +KW+ ALCEALF    K  CPFKDCSA +++  +G   DI
Sbjct: 138 NCKGVLELESCISLLPKELIDKWNDALCEALFCSVPKFYCPFKDCSAMLLDENEGVE-DI 197

Query: 145 LESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCP 204
            ESECP CHRLFCA C VPWH  + CEEYQ+    +   R  DD +++++A + +W RCP
Sbjct: 198 RESECPFCHRLFCASCHVPWHPGIGCEEYQKLNVDE---RGSDDLVVRELANQKQWRRCP 257

Query: 205 RCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHGGCRQ 244
            CKF+VEK  GCLHITCRC+FEFCYACG QWS  HGGC++
Sbjct: 258 TCKFYVEKNGGCLHITCRCRFEFCYACGEQWSSTHGGCQK 293

[Close]

BLAST of MC09g0400 vs. ExPASy TrEMBL
Match: A0A540L8Q3 (Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_031575 PE=3 SV=1)

HSP 1 Score: 258 bits (659), Expect = 2.76e-82
Identity = 116/220 (52.73%), Postives = 155/220 (70.45%), Query Frame = 0


Query: 23  SRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCP 82
           S +S +S  S++ LC IC+E K  D MF  E C+HSFC  C+ KHVA+KI      VSCP
Sbjct: 100 SGESSSSQPSTQTLCGICVEMKEADEMFRNEGCVHSFCSDCITKHVASKIQANIHVVSCP 159

Query: 83  SLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSA 142
            LDC   +E + C  MLPK V E+W+ ALCEA+ + A+++ CPF DCS  ++   +G  A
Sbjct: 160 GLDCRAVLELDACMPMLPKEVIERWNDALCEAMVLGAQRLYCPFSDCSTVLMIDNEGEEA 219

Query: 143 DILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMR 202
            + ESECPICHRLFCA+C VPWH  V CEEY+R  + + RGR D   ++K++A++ +W R
Sbjct: 220 -VRESECPICHRLFCARCEVPWHPGVDCEEYRRLNEDE-RGRAD--LMVKELAKQKKWKR 279

Query: 203 CPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHGGC 242
           CPRCK++VEKT GCLHITCRC+F+FCY CG++W+  HG C
Sbjct: 280 CPRCKYYVEKTQGCLHITCRCQFQFCYGCGAEWTSTHGVC 315

[Close]

BLAST of MC09g0400 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 4.6e-63
Identity = 108/243 (44.44%), Postives = 160/243 (65.84%), Query Frame = 0


Query: 5   ASSVGLSSTP------LTVPPENPSRKSKNS-SRSSRQLCEICLERKAIDNMFT-IEACL 64
           AS+VG++  P      +T+  + P  K++N     SR+LC IC++ K   ++F     C 
Sbjct: 56  ASTVGVNHHPQVHRNLVTLVKQEPEIKAENEPMEPSRRLCMICMDEKPSSDIFRGTTNCT 115

Query: 65  HSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALF 124
           H++C  C +++VA KI   +SR+ CP ++C+R +E  TC  ++PK V ++W++ LCE+L 
Sbjct: 116 HAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTCRDLIPKDVFDRWEKILCESLI 175

Query: 125 VEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQ 184
              +K  CPFKDCSA MVN E G  A++ ++EC  CHRLFC +C+V WHA + C+E+QR 
Sbjct: 176 SSWDKFYCPFKDCSAMMVNNENG-DANVTQTECRSCHRLFCVQCKVTWHAGIGCDEFQRF 235

Query: 185 KKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWS 240
             +KK+  ++DDALL  MA+  +W RCP CKF+V+K  GC HI CRC ++FCY CGS WS
Sbjct: 236 GNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEGCQHIKCRCGYQFCYGCGSVWS 295

[Close]

BLAST of MC09g0400 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 198.4 bits (503), Expect = 6.9e-51
Identity = 95/223 (42.60%), Postives = 139/223 (62.33%), Query Frame = 0


Query: 18  PPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSS 77
           PP +  +   +SS+++   CEIC++ K+I   F I  C H +C  CV K++AAK+     
Sbjct: 96  PPIHREKGQSSSSKTATFDCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNIL 155

Query: 78  RVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLE 137
            + CP   CS  +E + C  +LPK V ++W  ALCEA+ + ++K  CP+KDCS A+V LE
Sbjct: 156 SIECPVSGCSGRLEPDQCRQILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCS-ALVFLE 215

Query: 138 KGRSADILESECPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARK 197
           +     + +SECP CHR+ C +C   WH  +TCEE+Q+   + +RGR  DD LL  MA++
Sbjct: 216 ES-EVKMKDSECPHCHRMVCVECGTQWHPEMTCEEFQK-LAANERGR--DDILLATMAKQ 275

Query: 198 MEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGS-QWSHNH 240
            +W RCP CKF++EK+ GCL++ CRC   FCY CG+    H H
Sbjct: 276 KKWKRCPSCKFYIEKSQGCLYMKCRCGLAFCYNCGTPSRDHTH 313

[Close]

BLAST of MC09g0400 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 4.3e-37
Identity = 76/211 (36.02%), Postives = 113/211 (53.55%), Query Frame = 0


Query: 37  CEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLD--CSRFVEFET 96
           C IC ++   ++M   + CLH  C  CV + V  K+  G   +  P L+  C   +  E+
Sbjct: 181 CSICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSG---IVPPCLEDGCKSELTLES 240

Query: 97  CWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILE-SECPICH 156
           C  +L  ++ E W R + E L  +AEK+ CP++ CS  M   E  R A+      C  C 
Sbjct: 241 CSMVLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRACIKCS 300

Query: 157 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 216
            LFC  C+VPWH+ ++C +Y+R        R  +D +LK +A    W +C  CK  +E T
Sbjct: 301 ELFCIDCKVPWHSDLSCADYKRIHSE----RLVNDMMLKVLANDQMWRQCSECKHMIELT 360

Query: 217 YGCLHITCRCKFEFCYACGSQWS-HNHGGCR 244
            GC HITCRC +EFCY CG +W+ ++H  C+
Sbjct: 361 EGCNHITCRCGYEFCYRCGHKWTKYHHYSCQ 384

[Close]

BLAST of MC09g0400 vs. TAIR 10
Match: AT3G45470.1 (IBR domain containing protein )

HSP 1 Score: 149.4 bits (376), Expect = 3.7e-36
Identity = 70/208 (33.65%), Postives = 105/208 (50.48%), Query Frame = 0


Query: 35  QLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFET 94
           + C ICLE    D MF ++ CLH  C  CV + V  K+  G+   +C   +C   +  E 
Sbjct: 4   ETCVICLEETKADRMFVMDKCLHRHCYPCVNQLVEVKLRNGTV-PTCLDYECKLKLSLEN 63

Query: 95  CWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICHR 154
           C+ +L  +V E W   + E     A+++ CP+ +CS  M   E  RS    +  C  C  
Sbjct: 64  CFKVLKPKVIELWKHMMKEESIPLAKRIYCPYINCSTLMSKTEISRSNKSNDRACIKCSG 123

Query: 155 LFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKTY 214
           L C  C+VPWH+ ++C EY++           DD  LK +A   +W +C +C+  +E   
Sbjct: 124 LVCIDCKVPWHSDLSCAEYKKLHPDPVL----DDLTLKLLANDQKWRQCVKCRHLIELNQ 183

Query: 215 GCLHITCRCKFEFCYACGSQWSHNHGGC 243
           GC H+TCRC ++FCY CG +W      C
Sbjct: 184 GCNHMTCRCGYQFCYKCGVEWKKGQVTC 206

[Close]

BLAST of MC09g0400 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 149.1 bits (375), Expect = 4.8e-36
Identity = 77/236 (32.63%), Postives = 114/236 (48.31%), Query Frame = 0


Query: 15  LTVPPENPSRKSKNSSRSSRQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKING 74
           +  P E+P    + +    ++ C IC      + MF+++ C H FC +CV +HV  K+  
Sbjct: 283 MVTPHEDP----RQAKAVLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLH 342

Query: 75  GSSRVSCPSLDCSRFVEFETCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMV 134
           G +   CP   C   +  + C  +L  ++++ W + L E      E+V CP+  CSA M 
Sbjct: 343 GMA-PKCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMS 402

Query: 135 NLEKGRSADILES--------ECPICHRLFCAKCRVPWHARVTCEEYQRQKKSKKRGRED 194
             +   SA  L S         C  C  LFC  C+VPWH  ++C EY++           
Sbjct: 403 KTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPA---- 462

Query: 195 DDALLKDMARKMEWMRCPRCKFFVEKTYGCLHITCRCKFEFCYACGSQWSHNHGGC 243
           DD  LK +A    W +C +C+  +E + GC HITCRC  EFCY CG  W+   G C
Sbjct: 463 DDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGGGWNKIMGTC 509

The following BLAST results are available for this feature:

BLAST of MC09g0400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8TC41	6.6e-22	28.77	Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE...	[more]
D3YYI7	1.1e-21	28.30	Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P...	[more]
Q4KLT0	3.3e-21	26.79	Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ...	[more]
P0CE10	4.0e-19	28.71	ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
F4KGU4	1.2e-18	30.24	ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of MC09g0400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146079.1	9.76e-186	100.00	probable E3 ubiquitin-protein ligase RNF217 [Momordica charantia]	[more]
KAA0058684.1	2.44e-92	60.77	putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa]	[more]
TYK10490.1	9.89e-92	60.29	putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa]	[more]
KAE8646419.1	1.03e-87	61.62	hypothetical protein Csa_016113 [Cucumis sativus]	[more]
GAU14896.1	1.90e-84	53.42	hypothetical protein TSUD_46890 [Trifolium subterraneum]	[more]

BLAST of MC09g0400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CX43	4.73e-186	100.00	probable E3 ubiquitin-protein ligase RNF217 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A5A7UU31	1.18e-92	60.77	Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A5D3CGK1	4.79e-92	60.29	Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A2K3N511	5.60e-84	54.09	Putative E3 ubiquitin-protein ligase RNF144A-like protein OS=Trifolium pratense ...	[more]
A0A540L8Q3	2.76e-82	52.73	Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_031575 PE=3 SV=1	[more]

BLAST of MC09g0400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14250.1	4.6e-63	44.44	RING/U-box superfamily protein	[more]
AT3G53690.1	6.9e-51	42.60	RING/U-box superfamily protein	[more]
AT5G37560.1	4.3e-37	36.02	RING/U-box superfamily protein	[more]
AT3G45470.1	3.7e-36	33.65	IBR domain containing protein	[more]
AT5G60250.1	4.8e-36	32.63	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 187..242 e-value: 0.0089 score: 24.6 coord: 105..170 e-value: 5.1E-12 score: 55.9
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 107..170 e-value: 1.8E-9 score: 37.7 coord: 191..242 e-value: 1.6E-8 score: 34.6
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 37..84 e-value: 6.2E-4 score: 29.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 37..82 score: 10.099292
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 30..118 e-value: 1.6E-19 score: 71.6
None	No IPR available	GENE3D	1.20.120.1750		coord: 168..246 e-value: 3.4E-17 score: 65.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	PANTHER	PTHR11685:SF327	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 25..234
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 107..176
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 33..114
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 186..242
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 37..82 e-value: 5.5E-7 score: 29.4
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 25..234
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 55..64
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 33..246 score: 26.496796

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0400.1	MC09g0400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity