MC09g0378 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0378
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA glycosylase superfamily protein
LocationMC09: 3439458 .. 3443148 (-)
RNA-Seq ExpressionMC09g0378
SyntenyMC09g0378
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTGCTAACTACAGAGTAGATAAGTTTTTAGGAAATAATGAAGTGTTAATTTTTTTAACCGCGGGTCAAAACTAAGTATTATTTTATTTCTATAAAATTAGACAAATATCACCCAGCTCAACGCTCCCCCCACCCCACCGATTCCTACGTGGCAGTTTACGACTGGTCTGTTATTTTCAATTTCTCTCTCTCGATTCGTTGCTTTCTCCAGCAGGAAGGTCTCTCTCTCTCTCAACCCTCGAAAAAATTTTATCCCACTTTCCCATATTTTCCGTCTCTCTCTCTCTAGATTCTCTCTCTTGTTTCTCTTTTGATTTTCATACTCACAAACACAATCATGGCCGTTCCGAAAATTTCATCATCCACTTCCAGATAAGTGTTTCGCCCTTTGCTTTGAGCTCCTTTTTGTTCATCAATGTGGCTCCATTTTCCGGTCCCACCTAGGGTTCTGTTCTCTCACCTCACTCCCCAAACCCTACTGTTCAAACCCAATGAACTACACCCATTCTTTCTCATTTCTTTAATCTGAATCCAATCCCCAATTTGCCGAATTTTTTCATTGAAATATGTCAGGCCCTCCCAGAATCCGGTCGATGAATGTGGCGGATTCCGACTCCCGGCCGGTTCTTGGGCCTACCGGAAACAAAGCCCGACCTGTCGAGCCCAGAAAACCTGGTGGGAAGCCATTGAAGAAGCTTGAAAAGCCTCACCAGGAGGCTGAATCGAAGGACAAGAGGGTGCCATTGTCGCCGCCTCAATGCGTCTCGGTGCCATCGGTTCTGAGGCAGCAGGACCGCCACCAGGCGATTCTCAATCTCTCGATGAATGCGTCGTGTTCTTCCGATGCGTCGTCTGATTCGTTCAATAGCCGGGCGTCTAGCGCGAGAGGTACGAGGCAGCGCGGTCCCAATTTGAGGAGAAAGCAAAGTACGGTAAAGAGGGCTGAAAAGGCCGTTGAAAAGGTTGGCGTTGAAAGTGTGGTGGTGGTGGTGGATACAGTTGCTGGTTTAGAGCCAAAAAAACGATGTGCTTGGGTAACACCTAATACAGGTACAATGTGTTTAAATTTCTTGAATCTGCTTGTTCATTATGCTGGAACCATTTTTTCCTTTTGTTTCTGTTAGCTAAATGAGTTCAGGTGAAAATGGGTGTTCTTATGTTGACAGCATTGCTCTTCATTCAGAGATGAACAGTGTTGAGTTGAAATCTTCTGTTTAAGTACATGAAGGAATGAAGAACTGCAGTAATAAAATTTTGAGTTTGAATAAGCAATGTACAATTAGATCTTCGATTTAGGTCTAGTGAGTCATAATTTGCGGTTGACTCTAGCAGAGTATTTGCTATTCTGATCACTTATTTTCACTTATCTGGGAGTAGTCGATTCTCATTTCATTCTTCTTCCACCTCCTGCTATCTGATACGGCTCTTTATTACTGACTGTGCCCTGTACACTCAAAGAGAAATCATTGGGAATGGCGGCCATACTGTAGTTGCCACTTGCATGACTGTTACAAAATGTATAGTCCAAAAATCATTTTTTGATCCCTCAACTATGGGAATGGATTAGATGGCTGAATAATTTGCCACACCCTAGTGACCCTTCTCTACTTTTTATCTTCATCCTTACTGATGTGATATATTGTAGGTAATTTCAAACTGAAATCAATAATTGAAGGGGCTGATTTATGTGCTAGGTGTTTCTAGATTGTCTGTTGTCTTAGTTCAACTGTAATTTAGCCGTAAGTTACCTTTTCATTTTTGAAGAGTGGTCGTAATATCAACACTGAATCTTCTTTATCTTCCAAACTTTGTTAAGTCCTTTATTTATCAAGCTTTCATATAATCATATTACAATATTTGTTCACAATGGTTGGCAAAATATAGTTCGTGACTAGCAGAGGCTCTAGTTGTTGTAAAAGATACGATACGGGAATTATTTTATGTTTGTGAGAATTATTCACATATTTTCCCTCAACGCCTAACAATGTCAAGGTTGTACTCAGAAGGGTAAATTTTACTATCTTGTCTTTGTCTCTATCTTCTCTCTAATATAAGTCCATCCCCCTTCCCCCCCCAAAACAAAGAATTCATCTTGTCTCGATCTTCTTATTGACTATTCCAGCTTTTACACTTTTATCTTTTTTTTTTTGTCCATGGATTTTGTATCTAGTTTTGTATTGAGCAAATTTGGTTGCAATGTCAAATGTAATGTGCACCACGTAATATGTTGCTGGTTACTGCTGAAATGGCATATGCTTTACTCGATGCTCCCTTTTACATTCAGACTTTATAGTTTTAACTTCCATCTTTCTAAGACTTATCAAGTTATGTTGAGGCTCTAAAGCATGATTGCTTTTCTTCGTGCACCAGATCCATGTTATGCTGCTTTTCATGATGAAGAGTGGGGAGTACCTGTTCACGATGACAAGTATGTAACTATGTGATGAAGTTTCAGATTTTCTTCTTTCAATCCTTGCACTAAACTGCATTCAACTATTTCGATTTTTAACTTTAAAAAGATGAACATTTGTAGAAAATTGTTTGAACTGCTCTGCCTATCGGGTGCTTTGGCTGAACTTACATGGCCTGCTATCCTTAACAAAAGACATCTATTTAGGTATTACTTTACTTCCGAGTTCATTGTTTTTTCACATTATCATAGTACATGGCTTTTGCTGACTAATAATTATGCTATTTCCAATATTTCGTTGTTTTTTGAATTTTGACATAACCCTGTATAACGTGTGTACTTAAAGGGAAATCTTTTTGGACTTTGACCCAAATGCTGTTTCAAAATTAAACGAGAAAAAGATGGTTGCAGCTGGAAGTGCTGCTACCTCTTTACTGTCAGAACTTAAGGTGCGAGCTATCATTGAAAATGGTCGTCAAATGTGCAAGGTAGTTGCTAGATTCCTCTAGTCAAATTGTTTATTTATTCATTATGTTGCATAAAACGGACATATGGTCTATTTGAATCTGAAATGCGCTCTATTTGAGAACTCATGTGTCTATTCTCAAACCAATCTAAGAAGAGAGAGATGAGAGAAGAAAAGTCTTTAACTCTTATATTTGCCTCCTTTCCATTGTGCTGCAGGTAATCGATGAATTTGGTTCCTTCGACGTGTACATTTGGAACTTTGTCAACCACAAACCGATCATCAGTCAGTTTCGGTACCCACGCCAGGTCCCCGATAAGACCTCAAAAGCAGAGGTGATTAGCAAGGATCTCGTTAAGAGAGGGTTTCGTAGCGTGGGACCGACAGTCATCTATACATTCATGCAGGTGGCAGGGTTAACCAACGACCATCTCATCAGTTGCTTTAGGTTTCCAGAATGTATAGAGACAGCAGAGAGAGGAGAAAAGGATGGTGAAATCAAGCCTATTATTAACGAGAAAATACCAGAGGCTCTGAAAAACTTGGAACTATAAAGAAGCCATGGTCGCCTTTGGACCTTGCCTCAGTGTAATTAGCTACCAGAGTCCTTTCCTTTTTTTTTCTTCTTTTTTTCTTTTCTTCTGTAATGGCTTGTAAATTCCATGATGGGATATCTGCCACTTCCTCGGATGAGGTAAATTCTAGCAATGCTTTTGTGTATAAACTGACTCTCAGCTACAGAAGACAGCCTAGAATCAGTTCTGTTAGTTTACTACTTCAAGCATGTGGTTGGTTATTGCATTTAGAACCATG

mRNA sequence

CTTGTGCTAACTACAGAGTAGATAAGTTTTTAGGAAATAATGAAGTGTTAATTTTTTTAACCGCGGGTCAAAACTAAGTATTATTTTATTTCTATAAAATTAGACAAATATCACCCAGCTCAACGCTCCCCCCACCCCACCGATTCCTACGTGGCAGTTTACGACTGGTCTGTTATTTTCAATTTCTCTCTCTCGATTCGTTGCTTTCTCCAGCAGGAAGGTCTCTCTCTCTCTCAACCCTCGAAAAAATTTTATCCCACTTTCCCATATTTTCCGTCTCTCTCTCTCTAGATTCTCTCTCTTGTTTCTCTTTTGATTTTCATACTCACAAACACAATCATGGCCGTTCCGAAAATTTCATCATCCACTTCCAGATAAGTGTTTCGCCCTTTGCTTTGAGCTCCTTTTTGTTCATCAATGTGGCTCCATTTTCCGGTCCCACCTAGGGTTCTGTTCTCTCACCTCACTCCCCAAACCCTACTGTTCAAACCCAATGAACTACACCCATTCTTTCTCATTTCTTTAATCTGAATCCAATCCCCAATTTGCCGAATTTTTTCATTGAAATATGTCAGGCCCTCCCAGAATCCGGTCGATGAATGTGGCGGATTCCGACTCCCGGCCGGTTCTTGGGCCTACCGGAAACAAAGCCCGACCTGTCGAGCCCAGAAAACCTGGTGGGAAGCCATTGAAGAAGCTTGAAAAGCCTCACCAGGAGGCTGAATCGAAGGACAAGAGGGTGCCATTGTCGCCGCCTCAATGCGTCTCGGTGCCATCGGTTCTGAGGCAGCAGGACCGCCACCAGGCGATTCTCAATCTCTCGATGAATGCGTCGTGTTCTTCCGATGCGTCGTCTGATTCGTTCAATAGCCGGGCGTCTAGCGCGAGAGGTACGAGGCAGCGCGGTCCCAATTTGAGGAGAAAGCAAAGTACGGTAAAGAGGGCTGAAAAGGCCGTTGAAAAGGTTGGCGTTGAAAGTGTGGTGGTGGTGGTGGATACAGTTGCTGGTTTAGAGCCAAAAAAACGATGTGCTTGGGTAACACCTAATACAGATCCATGTTATGCTGCTTTTCATGATGAAGAGTGGGGAGTACCTGTTCACGATGACAAAAAATTGTTTGAACTGCTCTGCCTATCGGGTGCTTTGGCTGAACTTACATGGCCTGCTATCCTTAACAAAAGACATCTATTTAGGGAAATCTTTTTGGACTTTGACCCAAATGCTGTTTCAAAATTAAACGAGAAAAAGATGGTTGCAGCTGGAAGTGCTGCTACCTCTTTACTGTCAGAACTTAAGGTGCGAGCTATCATTGAAAATGGTCGTCAAATGTGCAAGGTAATCGATGAATTTGGTTCCTTCGACGTGTACATTTGGAACTTTGTCAACCACAAACCGATCATCAGTCAGTTTCGGTACCCACGCCAGGTCCCCGATAAGACCTCAAAAGCAGAGGTGATTAGCAAGGATCTCGTTAAGAGAGGGTTTCGTAGCGTGGGACCGACAGTCATCTATACATTCATGCAGGTGGCAGGGTTAACCAACGACCATCTCATCAGTTGCTTTAGGTTTCCAGAATGTATAGAGACAGCAGAGAGAGGAGAAAAGGATGGTGAAATCAAGCCTATTATTAACGAGAAAATACCAGAGGCTCTGAAAAACTTGGAACTATAAAGAAGCCATGGTCGCCTTTGGACCTTGCCTCAGTGTAATTAGCTACCAGAGTCCTTTCCTTTTTTTTTCTTCTTTTTTTCTTTTCTTCTGTAATGGCTTGTAAATTCCATGATGGGATATCTGCCACTTCCTCGGATGAGGTAAATTCTAGCAATGCTTTTGTGTATAAACTGACTCTCAGCTACAGAAGACAGCCTAGAATCAGTTCTGTTAGTTTACTACTTCAAGCATGTGGTTGGTTATTGCATTTAGAACCATG

Coding sequence (CDS)

ATGTCAGGCCCTCCCAGAATCCGGTCGATGAATGTGGCGGATTCCGACTCCCGGCCGGTTCTTGGGCCTACCGGAAACAAAGCCCGACCTGTCGAGCCCAGAAAACCTGGTGGGAAGCCATTGAAGAAGCTTGAAAAGCCTCACCAGGAGGCTGAATCGAAGGACAAGAGGGTGCCATTGTCGCCGCCTCAATGCGTCTCGGTGCCATCGGTTCTGAGGCAGCAGGACCGCCACCAGGCGATTCTCAATCTCTCGATGAATGCGTCGTGTTCTTCCGATGCGTCGTCTGATTCGTTCAATAGCCGGGCGTCTAGCGCGAGAGGTACGAGGCAGCGCGGTCCCAATTTGAGGAGAAAGCAAAGTACGGTAAAGAGGGCTGAAAAGGCCGTTGAAAAGGTTGGCGTTGAAAGTGTGGTGGTGGTGGTGGATACAGTTGCTGGTTTAGAGCCAAAAAAACGATGTGCTTGGGTAACACCTAATACAGATCCATGTTATGCTGCTTTTCATGATGAAGAGTGGGGAGTACCTGTTCACGATGACAAAAAATTGTTTGAACTGCTCTGCCTATCGGGTGCTTTGGCTGAACTTACATGGCCTGCTATCCTTAACAAAAGACATCTATTTAGGGAAATCTTTTTGGACTTTGACCCAAATGCTGTTTCAAAATTAAACGAGAAAAAGATGGTTGCAGCTGGAAGTGCTGCTACCTCTTTACTGTCAGAACTTAAGGTGCGAGCTATCATTGAAAATGGTCGTCAAATGTGCAAGGTAATCGATGAATTTGGTTCCTTCGACGTGTACATTTGGAACTTTGTCAACCACAAACCGATCATCAGTCAGTTTCGGTACCCACGCCAGGTCCCCGATAAGACCTCAAAAGCAGAGGTGATTAGCAAGGATCTCGTTAAGAGAGGGTTTCGTAGCGTGGGACCGACAGTCATCTATACATTCATGCAGGTGGCAGGGTTAACCAACGACCATCTCATCAGTTGCTTTAGGTTTCCAGAATGTATAGAGACAGCAGAGAGAGGAGAAAAGGATGGTGAAATCAAGCCTATTATTAACGAGAAAATACCAGAGGCTCTGAAAAACTTGGAACTATAA

Protein sequence

MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPLSPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQSTVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPEALKNLEL
Homology
BLAST of MC09g0378 vs. ExPASy Swiss-Prot
Match: Q7VG78 (Probable GMP synthase [glutamine-hydrolyzing] OS=Helicobacter hepaticus (strain ATCC 51449 / 3B1) OX=235279 GN=guaA PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.5e-41
Identity = 87/194 (44.85%), Postives = 111/194 (57.22%), Query Frame = 0

Query: 143 DTVAGLEPKKRCAWVTPNTDPC---YAAFHDEEWGVPVHDDKKLFELLCLSGALAELTWP 202
           D+  G+  K RCAW T   +     Y  +HD EWG P+H+DKKLFE L L G  A L+W 
Sbjct: 777 DSNEGVREKVRCAWATDKDEAARKLYEDYHDTEWGEPLHEDKKLFEHLVLEGFQAGLSWI 836

Query: 203 AILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLSELKVRAIIENGRQMCKVID 262
            IL KR  FR  F DFDP+ V+  +E K+         + +  K+ A I N +    V  
Sbjct: 837 TILKKREAFRVAFDDFDPHIVANYDEDKIKELMRNEGIIRNRAKIEAAIINAKAFMAVQR 896

Query: 263 EFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKDLVKRGFRSVGPTVIYTFMQ 322
           EFGSFD YIW FV  KPII+ F     +P  T  ++ I+KDL KRGF+ VG T +Y  MQ
Sbjct: 897 EFGSFDKYIWGFVGGKPIINAFESIADLPASTPLSDKIAKDLKKRGFKFVGTTTMYAMMQ 956

Query: 323 VAGLTNDHLISCFR 334
             G+ NDHL SCF+
Sbjct: 957 SIGMVNDHLTSCFK 970

BLAST of MC09g0378 vs. ExPASy Swiss-Prot
Match: P05100 (DNA-3-methyladenine glycosylase 1 OS=Escherichia coli (strain K12) OX=83333 GN=tag PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 76/184 (41.30%), Postives = 110/184 (59.78%), Query Frame = 0

Query: 152 KRCAWVTPNTDPCYAAFHDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREI 211
           +RC WV  + DP Y A+HD EWGVP  D KKLFE++CL G  A L+W  +L KR  +R  
Sbjct: 2   ERCGWV--SQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGLSWITVLKKRENYRAC 61

Query: 212 FLDFDPNAVSKLNEKKMVAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNF 271
           F  FDP  V+ + E+ +      A  +    K++AII N R   ++      F  ++W+F
Sbjct: 62  FHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAYLQMEQNGEPFVDFVWSF 121

Query: 272 VNHKPIISQFRYPRQVPDKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISC 331
           VNH+P ++Q     ++P  TS ++ +SK L KRGF+ VG T+ Y+FMQ  GL NDH++ C
Sbjct: 122 VNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTTICYSFMQACGLVNDHVVGC 181

Query: 332 FRFP 336
             +P
Sbjct: 182 CCYP 183

BLAST of MC09g0378 vs. ExPASy Swiss-Prot
Match: P44321 (DNA-3-methyladenine glycosylase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=tag PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.2e-33
Identity = 71/179 (39.66%), Postives = 99/179 (55.31%), Query Frame = 0

Query: 153 RCAWVTPNTDPCYAAFHDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIF 212
           RC WV       Y  +HD+EWG P  D +KLFE +CL G  A L+W  +L KR  +RE F
Sbjct: 4   RCPWV--GEQSIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSWITVLKKRESYREAF 63

Query: 213 LDFDPNAVSKLNEKKMVAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFV 272
             FDP  ++K+    + A    +  +    K+ AI++N +    +     +F  +IW+FV
Sbjct: 64  HQFDPKKIAKMTALDIDACMQNSGLIRHRAKLEAIVKNAKAYLAMEKCGENFSDFIWSFV 123

Query: 273 NHKPIISQFRYPRQVPDKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISC 332
           NHKPI++     R VP KT  ++ +SK L KRGF  +G T  Y FMQ  GL +DHL  C
Sbjct: 124 NHKPIVNDVPDLRSVPTKTEVSKALSKALKKRGFVFIGETTCYAFMQSMGLVDDHLNDC 180

BLAST of MC09g0378 vs. NCBI nr
Match: XP_022155202.1 (uncharacterized protein LOC111022341 [Momordica charantia])

HSP 1 Score: 724 bits (1869), Expect = 3.29e-263
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDD 180
           STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDD
Sbjct: 121 STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDD 180

Query: 181 KKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLS 240
           KKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLS
Sbjct: 181 KKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLS 240

Query: 241 ELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKD 300
           ELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKD
Sbjct: 241 ELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKD 300

Query: 301 LVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPE 360
           LVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPE
Sbjct: 301 LVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPE 360

Query: 361 ALKNLEL 367
           ALKNLEL
Sbjct: 361 ALKNLEL 367

BLAST of MC09g0378 vs. NCBI nr
Match: XP_008461179.1 (PREDICTED: probable GMP synthase [glutamine-hydrolyzing] [Cucumis melo] >KAA0058711.1 putative GMP synthase [Cucumis melo var. makuwa] >TYK10517.1 putative GMP synthase [Cucumis melo var. makuwa])

HSP 1 Score: 658 bits (1697), Expect = 6.01e-237
Identity = 340/370 (91.89%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RKPG KPLKKLEKP QE ESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKPGVKPLKKLEKPRQEVESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCV+VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVTVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 -STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180
            STVK A+KAVEKVGVESV VV DTV  LE KKRCAWVTPNTDPCYAAFHDEEWGVPVHD
Sbjct: 121 CSTVKGADKAVEKVGVESVAVVADTVGCLESKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180

Query: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLL 240
           DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDP  VSKLNEKKMVA GSAATSLL
Sbjct: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPTVVSKLNEKKMVAPGSAATSLL 240

Query: 241 SELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300
           SELK+RAIIENGRQMCKVIDEFGSF+VY+WNFVNHKPIISQFRYPRQVPDKTSKAEVISK
Sbjct: 241 SELKIRAIIENGRQMCKVIDEFGSFNVYMWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300

Query: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIET--AERGEKDGEIKPIINEK 360
           DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRF ECIET  AE+GE+DGE+K   NEK
Sbjct: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFTECIETQTAEKGERDGEMKLNPNEK 360

Query: 361 IPEALKNLEL 367
           +PEALKNLEL
Sbjct: 361 MPEALKNLEL 370

BLAST of MC09g0378 vs. NCBI nr
Match: XP_023514420.1 (uncharacterized protein LOC111778684 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 655 bits (1690), Expect = 6.75e-236
Identity = 337/369 (91.33%), Postives = 346/369 (93.77%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RK G KPLKKLEKPHQEAESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKSGVKPLKKLEKPHQEAESKDKRVPL 60

Query: 61  SPPQCVS-VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK 120
           SPPQCV+ VPSVLRQQDRHQAIL LSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK
Sbjct: 61  SPPQCVTTVPSVLRQQDRHQAILTLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK 120

Query: 121 QST-VKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVH 180
            S+ VKRAEKAVEKVG ESVV V +TV  LEPKKRCAWVT NTDPCYAAFHDEEWGVPVH
Sbjct: 121 SSSSVKRAEKAVEKVGAESVVAVANTVGCLEPKKRCAWVTSNTDPCYAAFHDEEWGVPVH 180

Query: 181 DDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSL 240
           DDKKLFELLCLSGALAELTWP IL KRHLFRE FLDFDPNAVSKLNEKKMVA GSAATSL
Sbjct: 181 DDKKLFELLCLSGALAELTWPTILKKRHLFRETFLDFDPNAVSKLNEKKMVAPGSAATSL 240

Query: 241 LSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVIS 300
           LSE KVRAIIENGRQMCKVIDEFGSF+VY+WNFVNHKP ISQFRYPRQVPDKTSKAEVIS
Sbjct: 241 LSEPKVRAIIENGRQMCKVIDEFGSFNVYVWNFVNHKPTISQFRYPRQVPDKTSKAEVIS 300

Query: 301 KDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKI 360
           KDLVKRGFRSVGPTVIYTFMQVAGLTNDHL+SCFRFPECIET E+GE+DG+IKP I EKI
Sbjct: 301 KDLVKRGFRSVGPTVIYTFMQVAGLTNDHLVSCFRFPECIETTEKGERDGDIKPTIIEKI 360

Query: 361 PEALKNLEL 367
           PEALKNLEL
Sbjct: 361 PEALKNLEL 369

BLAST of MC09g0378 vs. NCBI nr
Match: KAG6593364.1 (hypothetical protein SDJN03_12840, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655 bits (1690), Expect = 6.75e-236
Identity = 337/369 (91.33%), Postives = 345/369 (93.50%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RK G KPLKKLEKPHQEAESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKSGVKPLKKLEKPHQEAESKDKRVPL 60

Query: 61  SPPQCVS-VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK 120
           SPPQCV+ VPSVLRQQDRHQAIL LSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK
Sbjct: 61  SPPQCVTTVPSVLRQQDRHQAILTLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK 120

Query: 121 QS-TVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVH 180
            S TVKRAEKAVEKVG ESVV   +TV  LEPKKRCAWVT NTDPCYAAFHDEEWGVPVH
Sbjct: 121 SSSTVKRAEKAVEKVGAESVVAATNTVGCLEPKKRCAWVTSNTDPCYAAFHDEEWGVPVH 180

Query: 181 DDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSL 240
           DDKKLFELLCLSGALAELTWP IL KRHLFRE FLDFDPNAVSKLNEKKMVA GSAATSL
Sbjct: 181 DDKKLFELLCLSGALAELTWPTILKKRHLFRETFLDFDPNAVSKLNEKKMVAPGSAATSL 240

Query: 241 LSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVIS 300
           LSE KVRAIIENGRQMCKVIDEFGSF+VY+WNFVNHKP ISQFRYPRQVPDKTSKAEVIS
Sbjct: 241 LSEPKVRAIIENGRQMCKVIDEFGSFNVYVWNFVNHKPTISQFRYPRQVPDKTSKAEVIS 300

Query: 301 KDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKI 360
           KDLVKRGFRSVGPTVIYTFMQVAGLTNDHL+SCFRFPECIET E+GE+DG+IKP I EKI
Sbjct: 301 KDLVKRGFRSVGPTVIYTFMQVAGLTNDHLVSCFRFPECIETTEKGERDGDIKPTIIEKI 360

Query: 361 PEALKNLEL 367
           PEALKNLEL
Sbjct: 361 PEALKNLEL 369

BLAST of MC09g0378 vs. NCBI nr
Match: XP_004136097.2 (uncharacterized protein LOC101205558 [Cucumis sativus] >KGN45219.1 hypothetical protein Csa_016770 [Cucumis sativus])

HSP 1 Score: 655 bits (1690), Expect = 7.27e-236
Identity = 342/371 (92.18%), Postives = 350/371 (94.34%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RKPG KPLKKLEKP QE ESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKPGVKPLKKLEKPRQEVESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCV+VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVTVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 -STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180
            STVK A+KAVEKVGVESV VVVDTV  LE KKRCAWVTPNTDPCYAAFHDEEWGVPVHD
Sbjct: 121 CSTVKGADKAVEKVGVESVAVVVDTVGCLESKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180

Query: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLL 240
           DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDP AVSKLNEKKMVA GSAATSLL
Sbjct: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPTAVSKLNEKKMVAPGSAATSLL 240

Query: 241 SELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300
           SELKVRAIIENGRQMCKVIDEFGSF+VY+WNFVNHKPIISQFRYPRQVPDKTSKAEVISK
Sbjct: 241 SELKVRAIIENGRQMCKVIDEFGSFNVYMWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300

Query: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIET--AERGEKDG-EIKPIINE 360
           DLVKRGFRSVGPTVIYTFMQVAGLTNDHLI CFRF ECIET  AE+GE+DG E+K   NE
Sbjct: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLIGCFRFTECIETQTAEKGERDGGEMKLNPNE 360

Query: 361 KIPEALKNLEL 367
           K+PEALKNLEL
Sbjct: 361 KMPEALKNLEL 371

BLAST of MC09g0378 vs. ExPASy TrEMBL
Match: A0A6J1DNQ3 (uncharacterized protein LOC111022341 OS=Momordica charantia OX=3673 GN=LOC111022341 PE=4 SV=1)

HSP 1 Score: 724 bits (1869), Expect = 1.59e-263
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDD 180
           STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDD
Sbjct: 121 STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHDD 180

Query: 181 KKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLS 240
           KKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLS
Sbjct: 181 KKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLLS 240

Query: 241 ELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKD 300
           ELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKD
Sbjct: 241 ELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISKD 300

Query: 301 LVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPE 360
           LVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPE
Sbjct: 301 LVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKIPE 360

Query: 361 ALKNLEL 367
           ALKNLEL
Sbjct: 361 ALKNLEL 367

BLAST of MC09g0378 vs. ExPASy TrEMBL
Match: A0A5A7UYZ9 (Putative GMP synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001670 PE=4 SV=1)

HSP 1 Score: 658 bits (1697), Expect = 2.91e-237
Identity = 340/370 (91.89%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RKPG KPLKKLEKP QE ESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKPGVKPLKKLEKPRQEVESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCV+VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVTVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 -STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180
            STVK A+KAVEKVGVESV VV DTV  LE KKRCAWVTPNTDPCYAAFHDEEWGVPVHD
Sbjct: 121 CSTVKGADKAVEKVGVESVAVVADTVGCLESKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180

Query: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLL 240
           DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDP  VSKLNEKKMVA GSAATSLL
Sbjct: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPTVVSKLNEKKMVAPGSAATSLL 240

Query: 241 SELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300
           SELK+RAIIENGRQMCKVIDEFGSF+VY+WNFVNHKPIISQFRYPRQVPDKTSKAEVISK
Sbjct: 241 SELKIRAIIENGRQMCKVIDEFGSFNVYMWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300

Query: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIET--AERGEKDGEIKPIINEK 360
           DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRF ECIET  AE+GE+DGE+K   NEK
Sbjct: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFTECIETQTAEKGERDGEMKLNPNEK 360

Query: 361 IPEALKNLEL 367
           +PEALKNLEL
Sbjct: 361 MPEALKNLEL 370

BLAST of MC09g0378 vs. ExPASy TrEMBL
Match: A0A1S3CE52 (probable GMP synthase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103499838 PE=4 SV=1)

HSP 1 Score: 658 bits (1697), Expect = 2.91e-237
Identity = 340/370 (91.89%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RKPG KPLKKLEKP QE ESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKPGVKPLKKLEKPRQEVESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCV+VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVTVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 -STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180
            STVK A+KAVEKVGVESV VV DTV  LE KKRCAWVTPNTDPCYAAFHDEEWGVPVHD
Sbjct: 121 CSTVKGADKAVEKVGVESVAVVADTVGCLESKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180

Query: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLL 240
           DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDP  VSKLNEKKMVA GSAATSLL
Sbjct: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPTVVSKLNEKKMVAPGSAATSLL 240

Query: 241 SELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300
           SELK+RAIIENGRQMCKVIDEFGSF+VY+WNFVNHKPIISQFRYPRQVPDKTSKAEVISK
Sbjct: 241 SELKIRAIIENGRQMCKVIDEFGSFNVYMWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300

Query: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIET--AERGEKDGEIKPIINEK 360
           DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRF ECIET  AE+GE+DGE+K   NEK
Sbjct: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFTECIETQTAEKGERDGEMKLNPNEK 360

Query: 361 IPEALKNLEL 367
           +PEALKNLEL
Sbjct: 361 MPEALKNLEL 370

BLAST of MC09g0378 vs. ExPASy TrEMBL
Match: A0A0A0K8L6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432000 PE=4 SV=1)

HSP 1 Score: 655 bits (1690), Expect = 3.52e-236
Identity = 342/371 (92.18%), Postives = 350/371 (94.34%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RKPG KPLKKLEKP QE ESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKPGVKPLKKLEKPRQEVESKDKRVPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120
           SPPQCV+VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ
Sbjct: 61  SPPQCVTVPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRKQ 120

Query: 121 -STVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180
            STVK A+KAVEKVGVESV VVVDTV  LE KKRCAWVTPNTDPCYAAFHDEEWGVPVHD
Sbjct: 121 CSTVKGADKAVEKVGVESVAVVVDTVGCLESKKRCAWVTPNTDPCYAAFHDEEWGVPVHD 180

Query: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSLL 240
           DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDP AVSKLNEKKMVA GSAATSLL
Sbjct: 181 DKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPTAVSKLNEKKMVAPGSAATSLL 240

Query: 241 SELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300
           SELKVRAIIENGRQMCKVIDEFGSF+VY+WNFVNHKPIISQFRYPRQVPDKTSKAEVISK
Sbjct: 241 SELKVRAIIENGRQMCKVIDEFGSFNVYMWNFVNHKPIISQFRYPRQVPDKTSKAEVISK 300

Query: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIET--AERGEKDG-EIKPIINE 360
           DLVKRGFRSVGPTVIYTFMQVAGLTNDHLI CFRF ECIET  AE+GE+DG E+K   NE
Sbjct: 301 DLVKRGFRSVGPTVIYTFMQVAGLTNDHLIGCFRFTECIETQTAEKGERDGGEMKLNPNE 360

Query: 361 KIPEALKNLEL 367
           K+PEALKNLEL
Sbjct: 361 KMPEALKNLEL 371

BLAST of MC09g0378 vs. ExPASy TrEMBL
Match: A0A6J1H7A2 (uncharacterized protein LOC111461081 OS=Cucurbita moschata OX=3662 GN=LOC111461081 PE=4 SV=1)

HSP 1 Score: 651 bits (1679), Expect = 1.55e-234
Identity = 335/369 (90.79%), Postives = 344/369 (93.22%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSGPPRIRSMNVADSDSRPVLGPTGNKAR VE RK G KPLKKLEKPHQEAESKDKRVPL
Sbjct: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARTVETRKSGVKPLKKLEKPHQEAESKDKRVPL 60

Query: 61  SPPQCVS-VPSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK 120
           SPPQCV+ VPSVLRQQDRHQAIL LSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK
Sbjct: 61  SPPQCVTTVPSVLRQQDRHQAILTLSMNASCSSDASSDSFNSRASSARGTRQRGPNLRRK 120

Query: 121 QS-TVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHDEEWGVPVH 180
            S TVKRAEKAVEKVG ESVV   +TV  LEPKKRCAWVT NTDPCYAAFHDEEWGVPVH
Sbjct: 121 SSSTVKRAEKAVEKVGAESVVAATNTVGCLEPKKRCAWVTSNTDPCYAAFHDEEWGVPVH 180

Query: 181 DDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVAAGSAATSL 240
           DDKKLFELLCLSGALAELTWP IL KRHLFRE FLDFDPNAVSKLNEKKMVA GSAATSL
Sbjct: 181 DDKKLFELLCLSGALAELTWPTILKKRHLFRETFLDFDPNAVSKLNEKKMVAPGSAATSL 240

Query: 241 LSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDKTSKAEVIS 300
           LSE KVRAIIENGRQMCKVIDEFGSF+VY+WNFVNHKP ISQFRYPRQVPDKTSKA+VIS
Sbjct: 241 LSEPKVRAIIENGRQMCKVIDEFGSFNVYVWNFVNHKPTISQFRYPRQVPDKTSKADVIS 300

Query: 301 KDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERGEKDGEIKPIINEKI 360
           KDLVKRGFRSVGPTVIYTFMQVAGLTNDHL+SCFRF ECIET E+GE+DG+IKP I EKI
Sbjct: 301 KDLVKRGFRSVGPTVIYTFMQVAGLTNDHLVSCFRFQECIETTEKGERDGDIKPTIIEKI 360

Query: 361 PEALKNLEL 367
           PEALKNLEL
Sbjct: 361 PEALKNLEL 369

BLAST of MC09g0378 vs. TAIR 10
Match: AT5G57970.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.1e-100
Identity = 199/355 (56.06%), Postives = 245/355 (69.01%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSG PR++SMNVA++++R  LG T  KA P    K   K L+KLE+        D++   
Sbjct: 1   MSGAPRVQSMNVAEAETRSTLGSTAKKASPFITHKAVSKSLRKLERSSSGRTGSDEKTSY 60

Query: 61  SPP----------QCVSVPSVLRQQDRHQAIL--NLSMNASCSSDASSDSFNSRASSARG 120
           + P            ++  S+LR   RH+  L  NLS+NAS SSDAS DSF+SRAS+ R 
Sbjct: 61  ATPTETVSSSSQKHTLNAASILR---RHEQNLNSNLSLNASFSSDASMDSFHSRASTGRL 120

Query: 121 TRQRGPNLRRKQSTVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAF 180
            R      R K S   +    V +  ++S         G E KKRC WVTPN+DPCY  F
Sbjct: 121 IRSYSVGSRSK-SYPSKPRSVVSEGALDS------PPNGSETKKRCTWVTPNSDPCYIVF 180

Query: 181 HDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKM 240
           HDEEWGVPVHDDK+LFELL LSGALAE TWP IL+KR  FRE+F DFDPNA+ K+NEKK+
Sbjct: 181 HDEEWGVPVHDDKRLFELLVLSGALAEHTWPTILSKRQAFREVFADFDPNAIVKINEKKI 240

Query: 241 VAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVP 300
           +  GS A++LLS+LK+RA+IEN RQ+ KVI+E+GSFD YIW+FV +K I+S+FRY RQVP
Sbjct: 241 IGPGSPASTLLSDLKLRAVIENARQILKVIEEYGSFDKYIWSFVKNKAIVSKFRYQRQVP 300

Query: 301 DKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAER 344
            KT KAEVISKDLV+RGFRSVGPTV+Y+FMQ AG+TNDHL SCFRF  CI   ER
Sbjct: 301 AKTPKAEVISKDLVRRGFRSVGPTVVYSFMQAAGITNDHLTSCFRFHHCIFEHER 345

BLAST of MC09g0378 vs. TAIR 10
Match: AT5G57970.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.1e-100
Identity = 199/355 (56.06%), Postives = 245/355 (69.01%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MSG PR++SMNVA++++R  LG T  KA P    K   K L+KLE+        D++   
Sbjct: 1   MSGAPRVQSMNVAEAETRSTLGSTAKKASPFITHKAVSKSLRKLERSSSGRTGSDEKTSY 60

Query: 61  SPP----------QCVSVPSVLRQQDRHQAIL--NLSMNASCSSDASSDSFNSRASSARG 120
           + P            ++  S+LR   RH+  L  NLS+NAS SSDAS DSF+SRAS+ R 
Sbjct: 61  ATPTETVSSSSQKHTLNAASILR---RHEQNLNSNLSLNASFSSDASMDSFHSRASTGRL 120

Query: 121 TRQRGPNLRRKQSTVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAF 180
            R      R K S   +    V +  ++S         G E KKRC WVTPN+DPCY  F
Sbjct: 121 IRSYSVGSRSK-SYPSKPRSVVSEGALDS------PPNGSETKKRCTWVTPNSDPCYIVF 180

Query: 181 HDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKM 240
           HDEEWGVPVHDDK+LFELL LSGALAE TWP IL+KR  FRE+F DFDPNA+ K+NEKK+
Sbjct: 181 HDEEWGVPVHDDKRLFELLVLSGALAEHTWPTILSKRQAFREVFADFDPNAIVKINEKKI 240

Query: 241 VAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVP 300
           +  GS A++LLS+LK+RA+IEN RQ+ KVI+E+GSFD YIW+FV +K I+S+FRY RQVP
Sbjct: 241 IGPGSPASTLLSDLKLRAVIENARQILKVIEEYGSFDKYIWSFVKNKAIVSKFRYQRQVP 300

Query: 301 DKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAER 344
            KT KAEVISKDLV+RGFRSVGPTV+Y+FMQ AG+TNDHL SCFRF  CI   ER
Sbjct: 301 AKTPKAEVISKDLVRRGFRSVGPTVVYSFMQAAGITNDHLTSCFRFHHCIFEHER 345

BLAST of MC09g0378 vs. TAIR 10
Match: AT1G15970.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.6e-91
Identity = 197/355 (55.49%), Postives = 241/355 (67.89%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQE---AESKDKR 60
           MS PPR RS+N  + + R VLGPTGNK +    RKP G    KLEKP  E    +SKD++
Sbjct: 1   MSVPPRFRSVNSDEREFRSVLGPTGNKLQ----RKPPG---MKLEKPMMEKTIIDSKDEK 60

Query: 61  -----VPLSP----PQCVSV-PSVLRQQDRHQAILNLSMNASCSSDASSDSFNSRASSAR 120
                 P SP     QC S+  S+LR+        + SM AS SSDASS   +S  S A 
Sbjct: 61  AKKPTTPASPRTTLKQCSSLCSSILRKN-------SASMTASYSSDASSSCESSPLSVAS 120

Query: 121 GTRQRGPNLRRKQSTVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAA 180
            +  +   + R+  +V    K    VG E   V  D  A  + +KRCAW+TP  DPCY A
Sbjct: 121 SSSCK--KVVRRSGSVSSTRKL--SVGKEEEKVSGDCFA--DGRKRCAWITPKADPCYVA 180

Query: 181 FHDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKK 240
           FHDEEWGVPVHDDKKLFELLCLSGALAEL+W  IL++RH+ RE+F+DFDP AV++LN+KK
Sbjct: 181 FHDEEWGVPVHDDKKLFELLCLSGALAELSWTDILSRRHILREVFMDFDPVAVAELNDKK 240

Query: 241 MVAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQV 300
           + A G+AA SLLSE+K+R+I++N R + K+I E GS   Y+WNFVN+KP  SQFRY RQV
Sbjct: 241 LTAPGTAAISLLSEVKIRSILDNSRHVRKIIAECGSLKKYMWNFVNNKPTQSQFRYQRQV 300

Query: 301 PDKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAE 343
           P KTSKAE ISKDLV+RGFRSV PTVIY+FMQ AGLTNDHLI CFR+ +C   AE
Sbjct: 301 PVKTSKAEFISKDLVRRGFRSVSPTVIYSFMQAAGLTNDHLIGCFRYQDCCVDAE 335

BLAST of MC09g0378 vs. TAIR 10
Match: AT1G80850.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 3.0e-90
Identity = 188/354 (53.11%), Postives = 230/354 (64.97%), Query Frame = 0

Query: 1   MSGPPRIRSMNVADSDSRPVLGPTGNKARPVEPRKPGGKPLKKLEKPHQEAESKDKRVPL 60
           MS PPR+RS++ +D + R VLGP GNK +     KP  KP+ +  K     E   +  PL
Sbjct: 1   MSAPPRVRSVDSSDREFRSVLGPAGNKLQQKPLSKPVKKPVAEKTKNLTFTEKMPQCSPL 60

Query: 61  SPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSD------SFNSRASSARGTRQRG- 120
           SPP       +LR+         +SM AS SSDASS       S  S +S  R  R+ G 
Sbjct: 61  SPP-------ILRRN-------GISMTASYSSDASSSCESSPLSMTSTSSGKRVLRRSGS 120

Query: 121 ---PNLRRKQSTVKRAEKAVEKVGVESVVVVVDTVAGLEPKKRCAWVTPNTDPCYAAFHD 180
               +  R+  T +R EKA +                 + +KRCAW+TP +D CY AFHD
Sbjct: 121 VSSSSSLRRNLTEERDEKASD--------------CFCDGRKRCAWITPKSDQCYIAFHD 180

Query: 181 EEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFDPNAVSKLNEKKMVA 240
           EEWGVPVHDDK+LFELL LSGALAEL+W  IL+KR LFRE+F+DFDP A+S+L  KK+ +
Sbjct: 181 EEWGVPVHDDKRLFELLSLSGALAELSWKDILSKRQLFREVFMDFDPIAISELTNKKITS 240

Query: 241 AGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKPIISQFRYPRQVPDK 300
              AAT+LLSE K+R+I+EN  Q+CK+I  FGSFD YIWNFVN KP  SQFRYPRQVP K
Sbjct: 241 PEIAATTLLSEQKLRSILENANQVCKIIGAFGSFDKYIWNFVNQKPTQSQFRYPRQVPVK 300

Query: 301 TSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPECIETAERG 345
           TSKAE+ISKDLV+RGFRSV PTVIY+FMQ AGLTNDHL  CFR  +C+   E G
Sbjct: 301 TSKAELISKDLVRRGFRSVSPTVIYSFMQTAGLTNDHLTCCFRHHDCMTKDETG 326

BLAST of MC09g0378 vs. TAIR 10
Match: AT1G75090.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-67
Identity = 140/313 (44.73%), Postives = 191/313 (61.02%), Query Frame = 0

Query: 40  PLKKLEKPHQEAESKDKRVPLSPPQCVSVPSVLRQQDRHQAILNLSMNASCSSDASSDSF 99
           P+K +++      S   R  ++  +    P +  +  +  A      N S S+D SS S 
Sbjct: 10  PVKPIDESRAILCSTGNRFKVTKTEMTKKPQLNPRVTKSPATKKPDSNFSVSTDDSSSSS 69

Query: 100 NSRASSARGTRQRGPNLRRKQSTVKRAEKAVEKVG--VESVVVVVDTVAGLE-PKKRCAW 159
           +S   S+  T   G      + T       VEK+   V SV VV D    +  P KRC W
Sbjct: 70  SSSERSSVNTTNSG------KVTTPSKRNGVEKLNNVVASVAVVEDISPKIPGPVKRCHW 129

Query: 160 VTPNTDPCYAAFHDEEWGVPVHDDKKLFELLCLSGALAELTWPAILNKRHLFREIFLDFD 219
           +TPN+DP Y  FHDEEWGVPV DDKKLFELL  S ALAE +WP+IL +R  FR++F +FD
Sbjct: 130 ITPNSDPIYVLFHDEEWGVPVRDDKKLFELLVFSQALAEFSWPSILRRRDDFRKLFEEFD 189

Query: 220 PNAVSKLNEKKMVAAGSAATSLLSELKVRAIIENGRQMCKVIDEFGSFDVYIWNFVNHKP 279
           P+A+++  EK++++       +LSE K+RAI+EN + + KV  EFGSF  Y W FVNHKP
Sbjct: 190 PSAIAQFTEKRLMSLRVNGCLILSEQKLRAIVENAKSVLKVKQEFGSFSNYCWRFVNHKP 249

Query: 280 IISQFRYPRQVPDKTSKAEVISKDLVKRGFRSVGPTVIYTFMQVAGLTNDHLISCFRFPE 339
           + + +RY RQVP K+ KAE ISKD+++RGFR VGPTV+Y+F+Q +G+ NDHL +CFR+ E
Sbjct: 250 LRNGYRYGRQVPVKSPKAEYISKDMMQRGFRCVGPTVMYSFLQASGIVNDHLTACFRYQE 309

Query: 340 CIETAERGEKDGE 350
           C    ER  K  E
Sbjct: 310 CNVETERETKSHE 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7VG782.5e-4144.85Probable GMP synthase [glutamine-hydrolyzing] OS=Helicobacter hepaticus (strain ... [more]
P051001.1e-3841.30DNA-3-methyladenine glycosylase 1 OS=Escherichia coli (strain K12) OX=83333 GN=t... [more]
P443213.2e-3339.66DNA-3-methyladenine glycosylase OS=Haemophilus influenzae (strain ATCC 51907 / D... [more]
Match NameE-valueIdentityDescription
XP_022155202.13.29e-263100.00uncharacterized protein LOC111022341 [Momordica charantia][more]
XP_008461179.16.01e-23791.89PREDICTED: probable GMP synthase [glutamine-hydrolyzing] [Cucumis melo] >KAA0058... [more]
XP_023514420.16.75e-23691.33uncharacterized protein LOC111778684 [Cucurbita pepo subsp. pepo][more]
KAG6593364.16.75e-23691.33hypothetical protein SDJN03_12840, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_004136097.27.27e-23692.18uncharacterized protein LOC101205558 [Cucumis sativus] >KGN45219.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
A0A6J1DNQ31.59e-263100.00uncharacterized protein LOC111022341 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5A7UYZ92.91e-23791.89Putative GMP synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold45... [more]
A0A1S3CE522.91e-23791.89probable GMP synthase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0K8L63.52e-23692.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432000 PE=4 SV=1[more]
A0A6J1H7A21.55e-23490.79uncharacterized protein LOC111461081 OS=Cucurbita moschata OX=3662 GN=LOC1114610... [more]
Match NameE-valueIdentityDescription
AT5G57970.11.1e-10056.06DNA glycosylase superfamily protein [more]
AT5G57970.21.1e-10056.06DNA glycosylase superfamily protein [more]
AT1G15970.11.6e-9155.49DNA glycosylase superfamily protein [more]
AT1G80850.13.0e-9053.11DNA glycosylase superfamily protein [more]
AT1G75090.11.2e-6744.73DNA glycosylase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005019Methyladenine glycosylasePFAMPF03352Adenine_glycocoord: 160..333
e-value: 5.9E-62
score: 208.6
NoneNo IPR availableGENE3D1.10.340.30Hypothetical protein; domain 2coord: 151..335
e-value: 1.7E-71
score: 241.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..124
NoneNo IPR availablePANTHERPTHR31116:SF35GMP SYNTHASE [GLUTAMINE-HYDROLYZING]-RELATEDcoord: 1..349
NoneNo IPR availablePANTHERPTHR31116OS04G0501200 PROTEINcoord: 1..349
IPR011257DNA glycosylaseSUPERFAMILY48150DNA-glycosylasecoord: 152..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0378.1MC09g0378.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006284 base-excision repair
biological_process GO:0006281 DNA repair
molecular_function GO:0008725 DNA-3-methyladenine glycosylase activity
molecular_function GO:0003824 catalytic activity