MC09g0350 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0350
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RHOMBOID-like protein
Location	MC09: 3072448 .. 3075214 (-)
RNA-Seq Expression	MC09g0350
Synteny	MC09g0350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCGTATTTGACCCGACTTTATATATATATATATATATATTCACGTCCATGCATTAATACATGACGCAATAATCCATATGATAGAAGAGAAATCTTAAAAAGAGAATGAAAATGGGAGAAGGGAAGGGGGGCAATTAGGTAATATGAGAGTTTTCTATTAGCGGGAAAAAGTACCTAACTTAACGAACCACCCACACGCAGCATAGCCAATAGCAAGAATCGTTCGTTGGCTTTTGGCTATTTCCCCTCTTTCTCTCACTCTAACAACTGGTGCACAATAGCATCCCTCCCCTTTCCACGCTATTGTGCACTGCCTTCGATCCCCCGTCGCCTCCGCCGCCGCCGCAGCAATGGGGAAGAAGGTGTCGCCGCAGCCGCCGTATGACGACATCGAGTCGATGTCGTTCGAGGTCCGGAAGCCCCTGTCGATGCCGCCTCCGCCGAGATATTACCCGCCGCCGCCCAAGCCCTGGTTCCCCTGGCTGATGCCCCTCACATTTATCGCCAACATCGCCATGTTCGCCTTCACCATGTACCAGAACAATTGCCCCGCCGTCACCGGCCCCCGCCGCTGCCTCTTTTACCCCGAACTCGGCCGCTTCTCTTTCCAGCCCTTCCAAGAGAACCCCCTCCTCGGCCCTTCCATTAATATGTAACTCTCTAATCCCTCTCTCTTTCCCATCAATTCTTCTTCGTTTTTATGCAATTTTGAAATATGGGTTTTGTTTATCTCGATGAAATTTCGTAACCCTTATACAGAAAACAAAATTTTCTGGTTTCTGCACAAGGGGGAAAGGGGACCAACTTTTTCTTGCTTTTTTGTTTTCATGAATCTGTTGGATCATTCTTATTCATCTAGTACCAAATTAATTGGGGGAGGATATGACGTAATTACTGTAGTTTATATATACAGAATCCCTTGCTCTTGTATCTATGTTAAATCATCTCTCGACCTGAAATGTTTCGTTGTTTAAGTTGGTAGATTACAGCAAATTTAATTCTTGTTTTTCATATTCTAAATATTTTCCAGGGTTTTGAGCTTGGAGCATTAACACAAAACTCAACAACTGATTGTTCGATGATGTGTTAAATTTAACTTTTTATTCATATTTTCAATACTTTCCTTGCCTGGGAGCTTGAAAATTAACACACAAGAGGATCAATATCAATTGAGGAGGAAATTATATAATTTCATGTATCGGAACTCCTGTTTTCATACTATATTAGTCGCTCAACCCAAAAACTTATGTTCATTTATTCATATTTTTAATACTCTCCATCGAACTTGATATTTAGCACAAGGCTCAACGCAAGTGATTATCGATATTGGTTGAGAAGGAAATGATGCAAGTTTGAATACCGAACCTTTTGTTTTGTTTTTTTTTTTCTTCTTATTATTGATATTTAAGCTGTTGGAAAATGGTGTTGCAGATTGAAACGATATGGAGCACTGGACAGAGAGTCAGTAGTGGAGCATGGCGAAGGGTGGCGCCTGATTTCCTGTATCTGGCTTCATGCTGGAGTTGTTCATTTACTTGCCAATATGCTCAGCCTTCTCTTTGTAGGAATTCGACTCGAACAAGAATTTGGATTTTGTAAGCACTTCTGCAACTATTGATTAAGATTTAAACAGTCATAGTTTCATTTTTCATGACTTTCTGCACCTCCTAAAGGGAAAACAGAGATGTCTCTGTTTTTAAGGTGGGTTTGGAGTAATTTTATATAAAAAAAGTGCTTTCATGTGAAAGCAGTTGCAGCTTCTGACTTTGCCTTGTGTTTTTAGACTGATTTTGAAATGGATTTTGTTGGGTTTGCAAAGTGAATGGAAACAAAGTACTATATTGACTAGGTAAGGAAATGATCCATAGTATATAGATGAGGATAATTATCTCTATTGATATGAGACTTTTTAGATGAAACCAAAAGTAAAATCACGAGAGTTATGTTCAAAACGGACAATATTATATCATTGTGGAGATATTTGGGAGTACGATGATACCATTTTCCTTAACAGGTTTTAAAGAAAATGTTCGGAAAGGGATTTAAGGTCATTTTTTCACCGTTTCAAAAGTCATTCTAAACTTACTCTGAAAGAATCTCTCGAAACCGATTTGATGATTCTTTTTTGGATTTGGCAGTGCGAATAGGCTTTTTGTATCTGCTGTCTGGATTTGGTGGGAGTTTACTGTCATCTCTGAATATCAATCCAGTAGAAAATCCAACCATATCAGTTGGTGCTTCTGGTGCACTTTTTGGGCTTTTGGGAGCAATGCTTTCTGAGCTTATCACAAACTGGACAATATATTCAAATAAGGTACCAAAAAGCTTAATTTTCCATTCCAATTTTCTATATTATATCACAAAAGACTGCGTTACACATCGGTCAATAGGAGATTTTATTTGGAACGCAATAGTAATAGACCAATAAAAATATGCCACATCTAAAAGTAACTCGAAATGCTCTTAAAGATTTTGCACTCTCATGATCTGTTTGTTAATGGATCTTCAGTGTGCAGCCATGATGTCTTTGATTCTGGTCATTGCCTTAAACTTGGCAGTCGGGTTTGTGCCTCACGTTGACAACTCCGCGCACATCGGAGGCTTCGTCTCGGGCTTTCTCCTTGGCTTTGTCCTCCTAATGCGACCGCAGTTCGGATACGTTAACCGCAAGTACATCCCTGCAGGGTCCGACGTGAAACGAACAGCCAAGCACCAATGTTACCAGTACTTCTTATTGGTTTTAGCTCTAGGCCTCTTGATTTTCGGG

mRNA sequence

CCCGTATTTGACCCGACTTTATATATATATATATATATATTCACGTCCATGCATTAATACATGACGCAATAATCCATATGATAGAAGAGAAATCTTAAAAAGAGAATGAAAATGGGAGAAGGGAAGGGGGGCAATTAGGTAATATGAGAGTTTTCTATTAGCGGGAAAAAGTACCTAACTTAACGAACCACCCACACGCAGCATAGCCAATAGCAAGAATCGTTCGTTGGCTTTTGGCTATTTCCCCTCTTTCTCTCACTCTAACAACTGGTGCACAATAGCATCCCTCCCCTTTCCACGCTATTGTGCACTGCCTTCGATCCCCCGTCGCCTCCGCCGCCGCCGCAGCAATGGGGAAGAAGGTGTCGCCGCAGCCGCCGTATGACGACATCGAGTCGATGTCGTTCGAGGTCCGGAAGCCCCTGTCGATGCCGCCTCCGCCGAGATATTACCCGCCGCCGCCCAAGCCCTGGTTCCCCTGGCTGATGCCCCTCACATTTATCGCCAACATCGCCATGTTCGCCTTCACCATGTACCAGAACAATTGCCCCGCCGTCACCGGCCCCCGCCGCTGCCTCTTTTACCCCGAACTCGGCCGCTTCTCTTTCCAGCCCTTCCAAGAGAACCCCCTCCTCGGCCCTTCCATTAATATATTGAAACGATATGGAGCACTGGACAGAGAGTCAGTAGTGGAGCATGGCGAAGGGTGGCGCCTGATTTCCTGTATCTGGCTTCATGCTGGAGTTGTTCATTTACTTGCCAATATGCTCAGCCTTCTCTTTGTAGGAATTCGACTCGAACAAGAATTTGGATTTTTGCGAATAGGCTTTTTGTATCTGCTGTCTGGATTTGGTGGGAGTTTACTGTCATCTCTGAATATCAATCCAGTAGAAAATCCAACCATATCAGTTGGTGCTTCTGGTGCACTTTTTGGGCTTTTGGGAGCAATGCTTTCTGAGCTTATCACAAACTGGACAATATATTCAAATAAGTGTGCAGCCATGATGTCTTTGATTCTGGTCATTGCCTTAAACTTGGCAGTCGGGTTTGTGCCTCACGTTGACAACTCCGCGCACATCGGAGGCTTCGTCTCGGGCTTTCTCCTTGGCTTTGTCCTCCTAATGCGACCGCAGTTCGGATACGTTAACCGCAAGTACATCCCTGCAGGGTCCGACGTGAAACGAACAGCCAAGCACCAATGTTACCAGTACTTCTTATTGGTTTTAGCTCTAGGCCTCTTGATTTTCGGG

Coding sequence (CDS)

ATGGGGAAGAAGGTGTCGCCGCAGCCGCCGTATGACGACATCGAGTCGATGTCGTTCGAGGTCCGGAAGCCCCTGTCGATGCCGCCTCCGCCGAGATATTACCCGCCGCCGCCCAAGCCCTGGTTCCCCTGGCTGATGCCCCTCACATTTATCGCCAACATCGCCATGTTCGCCTTCACCATGTACCAGAACAATTGCCCCGCCGTCACCGGCCCCCGCCGCTGCCTCTTTTACCCCGAACTCGGCCGCTTCTCTTTCCAGCCCTTCCAAGAGAACCCCCTCCTCGGCCCTTCCATTAATATATTGAAACGATATGGAGCACTGGACAGAGAGTCAGTAGTGGAGCATGGCGAAGGGTGGCGCCTGATTTCCTGTATCTGGCTTCATGCTGGAGTTGTTCATTTACTTGCCAATATGCTCAGCCTTCTCTTTGTAGGAATTCGACTCGAACAAGAATTTGGATTTTTGCGAATAGGCTTTTTGTATCTGCTGTCTGGATTTGGTGGGAGTTTACTGTCATCTCTGAATATCAATCCAGTAGAAAATCCAACCATATCAGTTGGTGCTTCTGGTGCACTTTTTGGGCTTTTGGGAGCAATGCTTTCTGAGCTTATCACAAACTGGACAATATATTCAAATAAGTGTGCAGCCATGATGTCTTTGATTCTGGTCATTGCCTTAAACTTGGCAGTCGGGTTTGTGCCTCACGTTGACAACTCCGCGCACATCGGAGGCTTCGTCTCGGGCTTTCTCCTTGGCTTTGTCCTCCTAATGCGACCGCAGTTCGGATACGTTAACCGCAAGTACATCCCTGCAGGGTCCGACGTGAAACGAACAGCCAAGCACCAATGTTACCAGTACTTCTTATTGGTTTTAGCTCTAGGCCTCTTGATTTTCGGG

Protein sequence

MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG

Homology

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BLAST of MC09g0350 vs. ExPASy Swiss-Prot
Match: Q9SSR0 (RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.6e-92
Identity = 181/303 (59.74%), Postives = 222/303 (73.27%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPP--PRYYPPPPKPWFPWLMPLTFIANIAMFA 60
           MGK+  P PP  DIE+           PPP  P + PP P PW  WL+PL   AN   FA
Sbjct: 1   MGKR-PPIPP--DIEN---------GPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFA 60

Query: 61  FTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGE 120
            TMY N+CPA +    CL +  LGR SFQP +EN LLGPSI  L++ GAL+R  +VE GE
Sbjct: 61  TTMYVNDCPARSD--ECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERR-LVEEGE 120

Query: 121 GWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNIN 180
            WRLISCIWLH G +HL+ANM+SL+ +G+RLEQEFGF+RIG LY++SG GGSL+S L  +
Sbjct: 121 RWRLISCIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVSCLTDS 180

Query: 181 PVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVD 240
             E   +SVGASGALFGLLGAMLSELITNWTIY NKC A+M+LIL+I LNL+VGF+P VD
Sbjct: 181 QGER--VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVD 240

Query: 241 NSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVK-RTAKHQCYQYFLLVLALGLL 300
           NSAH GGF++GF LGFVLL+RPQ+GYVN KYIP G D+K + +KH+CYQ+     +L +L
Sbjct: 241 NSAHFGGFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAIL 286

[Close]

BLAST of MC09g0350 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.1e-86
Identity = 166/265 (62.64%), Postives = 201/265 (75.85%), Query Frame = 0


Query: 37  PPKPWFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLG 96
           P K WFPWL+P   +ANIA+FA +M+ NNCP  +      F   LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSAYCLARF---LGRFAFQPMKENPLLG 109

Query: 97  PSINILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFL 156
           PS   L++ GALD   VV   E WRL +CIWLHAGV H+LANMLSL+F+GIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 157 RIGFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCA 216
           RIG LY++SGFGGSLLSSL         ISVGASGALFGLLGAMLSEL+TNWTIY+NK A
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 217 AMMSLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAG-SD 276
           A+++LI +IA+NLAVG +PHVDN AH+GGF SGFLLGFV L+RPQ+GY N++  P G + 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 277 VKRTAKHQCYQYFLLVLALGLLIFG 301
               +KH+ YQY L + +L LLI G
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAG 307

[Close]

BLAST of MC09g0350 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 2.6e-81
Identity = 154/263 (58.56%), Postives = 200/263 (76.05%), Query Frame = 0


Query: 41  WFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFY---PELGRFSFQPFQENPLLGP 100
           W  WL+P+  +AN+A+F   M+ NNCP      R   +     LGR SF+P + NPL GP
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFGP 112

Query: 101 SINILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLR 160
           S + L++ GAL+   VVE  EGWRL++CIWLHAGV+HL ANMLSL+F+GIRLEQ+FGF+R
Sbjct: 113 SSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVR 172

Query: 161 IGFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAA 220
           IG +YLLSG GGS+LSSL I      +ISVGASGALFGLLG+MLSEL TNWTIYSNK AA
Sbjct: 173 IGVIYLLSGIGGSVLSSLFI----RNSISVGASGALFGLLGSMLSELFTNWTIYSNKIAA 232

Query: 221 MMSLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVK 280
           +++L+ VI +NLA+G +PHVDN AH+GGFV+GFLLGF+LL RPQF ++ R+++P G+ ++
Sbjct: 233 LLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPLR 292

Query: 281 RTAKHQCYQYFLLVLALGLLIFG 301
              K++ YQY L +L+L LLI G
Sbjct: 293 --YKYKTYQYLLWLLSLVLLIAG 309

[Close]

BLAST of MC09g0350 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.9e-80
Identity = 148/262 (56.49%), Postives = 199/262 (75.95%), Query Frame = 0


Query: 41  WFPWLMPLTFIANIAMFAFTMYQNNCP-AVTGPRRCLFYPELGRFSFQPFQENPLLGPSI 100
           W  WL+P   +AN+A+F   M+ N+CP  +TGP +      LGRFSFQP +ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 101 NILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIG 160
           + L++ GAL+   VV   +GWRL+SC+WLHAG++HLL NMLSL+F+GIRLEQ+FGF+R+G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 161 FLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMM 220
            +YL+SG GGS+LSSL +      +ISVGASGALFGLLGAMLSEL+TNWTIY+NK AA++
Sbjct: 151 LIYLISGLGGSILSSLFL----QESISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 221 SLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRT 280
           +L+ +IA+NLA+G +P VDN AHIGGF++GF LGFVLL+RPQ+G+   +     ++  RT
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR-----TNTSRT 270

Query: 281 -AKHQCYQYFLLVLALGLLIFG 301
             K+  YQY L V+++ LL+ G
Sbjct: 271 KRKYSMYQYVLFVVSVVLLVVG 283

[Close]

BLAST of MC09g0350 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.3e-77
Identity = 146/262 (55.73%), Postives = 191/262 (72.90%), Query Frame = 0


Query: 39  KPWFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPS 98
           + WFPWL+P   +AN+A+F  TMY NNCP  +G     F   LGRFSFQ  +ENPLLGPS
Sbjct: 62  RSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGD---CFADFLGRFSFQNTRENPLLGPS 121

Query: 99  INILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRI 158
              L+  G LD + VV+  EGWRL+SC WLH GVVHLL NML+LLF+GIR+E+EFGF+RI
Sbjct: 122 SLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRI 181

Query: 159 GFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAM 218
           G LYL+SGFGGS+LS+L +       ISVGASGA+FGLLG MLSE+  NWTIYSNK   +
Sbjct: 182 GLLYLISGFGGSILSALFL----RSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTI 241

Query: 219 MSLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKR 278
           ++L+L++A+NL +G +P VDN AHIGGF +GFLLGFVLL+RP +G++N++  P      +
Sbjct: 242 VTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGWINQRNGPGA----K 301

Query: 279 TAKHQCYQYFLLVLALGLLIFG 301
             + + YQ  L  ++L +L+ G
Sbjct: 302 PHRFKIYQGILWTISLLILVAG 312

[Close]

BLAST of MC09g0350 vs. NCBI nr
Match: XP_022147001.1 (RHOMBOID-like protein 5 [Momordica charantia])

HSP 1 Score: 608 bits (1567), Expect = 4.55e-219
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT
Sbjct: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW
Sbjct: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV
Sbjct: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG
Sbjct: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300

[Close]

BLAST of MC09g0350 vs. NCBI nr
Match: XP_023514894.1 (RHOMBOID-like protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527 bits (1358), Expect = 2.58e-187
Identity = 253/300 (84.33%), Postives = 280/300 (93.33%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+  P     DIESMS+EVRKPLSMPPPP YYPPPPKPWFPWL+PLTF+ANIAMFAFT
Sbjct: 1   MGKRSPP----GDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFAFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA  G  RCLFYPELGRFSFQPFQENPLLGPSI  LKRYGALD+E+VVEHGEGW
Sbjct: 61  LYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSITTLKRYGALDKEAVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFL+IG LY+LSGFGGSLLSSL+INP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPS 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAA +SLIL+IALNLAVGF+PH+DNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGF+LL++PQFGYVN KYIPAG+DVKR +KH+CYQYFLL++AL LLIFG
Sbjct: 241 AHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFG 296

[Close]

BLAST of MC09g0350 vs. NCBI nr
Match: XP_022964405.1 (RHOMBOID-like protein 5 [Cucurbita moschata] >KAG6593439.1 RHOMBOID-like protein 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025779.1 RHOMBOID-like protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526 bits (1356), Expect = 5.21e-187
Identity = 252/300 (84.00%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+  P     DIESMS+EVRKPLSMPPPP YYPPPPKPWFPWL+PLTF+ANIAMF+FT
Sbjct: 1   MGKRSPP----GDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA  G  RCLFYPELGRFSFQPFQENPLLGPSI+ LKRYGALD+E+VVEHGEGW
Sbjct: 61  LYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFL+IG LY+LSGFGGSLLSSL+INP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPS 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAA +SLIL+IALNLAVGF+PH+DNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGF+LL++PQFGYVN KYIPAG+DVKR +KH+CYQYFLL++AL LLIFG
Sbjct: 241 AHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFG 296

[Close]

BLAST of MC09g0350 vs. NCBI nr
Match: XP_023000373.1 (RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 523 bits (1346), Expect = 1.74e-185
Identity = 250/300 (83.33%), Postives = 279/300 (93.00%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+  P     DIESMS++VRKPLSMPPPP YYPPPPKPWFPWL+PLTF+ANIAMF FT
Sbjct: 1   MGKRSPP----GDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA  G  RCLFYPELGRFSFQPFQENPLLGPSI+ LKRYGALD+E+VVEHGEGW
Sbjct: 61  LYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFL+IG LY+LSGFGGSLLSSL+INP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPS 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENP ISVGASGALFGLLGAMLSELITNWTIYSNKCAA +SLIL+IALNLAVGF+PH+DNS
Sbjct: 181 ENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGF+LL++PQFGYVN KYIPAG+DVKR +KH+CYQYFLL++AL LLIFG
Sbjct: 241 AHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFG 296

[Close]

BLAST of MC09g0350 vs. NCBI nr
Match: XP_011659795.2 (RHOMBOID-like protein 5 [Cucumis sativus] >KAE8646443.1 hypothetical protein Csa_015571 [Cucumis sativus])

HSP 1 Score: 509 bits (1310), Expect = 5.30e-180
Identity = 238/300 (79.33%), Postives = 275/300 (91.67%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+    PP   +ESMS++V KP+SMPPPP YYPPPPKPWFPWL+PL F+ N+ MF F 
Sbjct: 1   MGKR----PPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFM 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA++G  RCL YPELGRFSFQPF ENPLLGPSI ILKRYGALD+E+V+E GEGW
Sbjct: 61  LYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFLRIG LY+LSGFGGSLLSS+++NP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPA 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIY+NKCAA++SLIL+IALNLAVGF+PHVDNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGF+SGFLLGF+LL+RPQFGYVN+KYIPAGSDVKR +KH+CYQY LL++AL LL+FG
Sbjct: 241 AHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296

[Close]

BLAST of MC09g0350 vs. ExPASy TrEMBL
Match: A0A6J1D113 (RHOMBOID-like protein OS=Momordica charantia OX=3673 GN=LOC111016051 PE=3 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 2.20e-219
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT
Sbjct: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW
Sbjct: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV
Sbjct: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG
Sbjct: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300

[Close]

BLAST of MC09g0350 vs. ExPASy TrEMBL
Match: A0A6J1HKQ1 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1)

HSP 1 Score: 526 bits (1356), Expect = 2.52e-187
Identity = 252/300 (84.00%), Postives = 281/300 (93.67%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+  P     DIESMS+EVRKPLSMPPPP YYPPPPKPWFPWL+PLTF+ANIAMF+FT
Sbjct: 1   MGKRSPP----GDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA  G  RCLFYPELGRFSFQPFQENPLLGPSI+ LKRYGALD+E+VVEHGEGW
Sbjct: 61  LYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFL+IG LY+LSGFGGSLLSSL+INP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPS 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAA +SLIL+IALNLAVGF+PH+DNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGF+LL++PQFGYVN KYIPAG+DVKR +KH+CYQYFLL++AL LLIFG
Sbjct: 241 AHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFG 296

[Close]

BLAST of MC09g0350 vs. ExPASy TrEMBL
Match: A0A6J1KMF5 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 8.42e-186
Identity = 250/300 (83.33%), Postives = 279/300 (93.00%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+  P     DIESMS++VRKPLSMPPPP YYPPPPKPWFPWL+PLTF+ANIAMF FT
Sbjct: 1   MGKRSPP----GDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFT 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA  G  RCLFYPELGRFSFQPFQENPLLGPSI+ LKRYGALD+E+VVEHGEGW
Sbjct: 61  LYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFL+IG LY+LSGFGGSLLSSL+INP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPS 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENP ISVGASGALFGLLGAMLSELITNWTIYSNKCAA +SLIL+IALNLAVGF+PH+DNS
Sbjct: 181 ENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGFVSGFLLGF+LL++PQFGYVN KYIPAG+DVKR +KH+CYQYFLL++AL LLIFG
Sbjct: 241 AHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFG 296

[Close]

BLAST of MC09g0350 vs. ExPASy TrEMBL
Match: A0A0A0K8T6 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G432610 PE=3 SV=1)

HSP 1 Score: 509 bits (1310), Expect = 1.19e-180
Identity = 238/300 (79.33%), Postives = 275/300 (91.67%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAFT 60
           MGK+    PP   +ESMS++V KP+SMPPPP YYPPPPKPWFPWL+PL F+ N+ MF F 
Sbjct: 1   MGKR----PPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFM 60

Query: 61  MYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEGW 120
           +Y+NNCPA++G  RCL YPELGRFSFQPF ENPLLGPSI ILKRYGALD+E+V+E GEGW
Sbjct: 61  LYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGW 120

Query: 121 RLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINPV 180
           RL+SC+WLHAGV+HLLANMLSLLF+GIRLEQEFGFLRIG LY+LSGFGGSLLSS+++NP 
Sbjct: 121 RLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPA 180

Query: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDNS 240
           ENPTISVGASGALFGLLGAMLSELITNWTIY+NKCAA++SLIL+IALNLAVGF+PHVDNS
Sbjct: 181 ENPTISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNS 240

Query: 241 AHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIFG 300
           AHIGGF+SGFLLGF+LL+RPQFGYVN+KYIPAGSDVKR +KH+CYQY LL++AL LL+FG
Sbjct: 241 AHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296

[Close]

BLAST of MC09g0350 vs. ExPASy TrEMBL
Match: A0A1S3CDZ1 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103499793 PE=3 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 2.91e-179
Identity = 238/301 (79.07%), Postives = 275/301 (91.36%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLS-MPPPPRYYPPPPKPWFPWLMPLTFIANIAMFAF 60
           MGK+    PP  D+ESMS+ + KP++ MPPPP YYPPPPKPWFPWL+PL F+ N+ MF F
Sbjct: 1   MGKR----PPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIF 60

Query: 61  TMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGEG 120
           T+Y+NNCPA+TG  RCL YPELGRFSFQPF ENPLLGP+I+IL+RYGALD+E+VVE GEG
Sbjct: 61  TLYKNNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEG 120

Query: 121 WRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNINP 180
           WRL+SC WLHAGV+HLLANMLSLLF+G+RLEQEFGFL+IG LY+LSGFGGSLLSS+++NP
Sbjct: 121 WRLVSCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNP 180

Query: 181 VENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVDN 240
            ENPTISVGASGALFGLLGAMLSELITNWTIY+NKCAA+MSLIL+IALNLAVGFVPHVDN
Sbjct: 181 AENPTISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDN 240

Query: 241 SAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRTAKHQCYQYFLLVLALGLLIF 300
           SAHIGGF+SGFLLGF+LL+RPQFGYVN KYIP GSDVKR +KH+CYQYFLL++AL LL+F
Sbjct: 241 SAHIGGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVF 297

[Close]

BLAST of MC09g0350 vs. TAIR 10
Match: AT1G52580.1 (RHOMBOID-like protein 5 )

HSP 1 Score: 339.0 bits (868), Expect = 4.0e-93
Identity = 181/303 (59.74%), Postives = 222/303 (73.27%), Query Frame = 0


Query: 1   MGKKVSPQPPYDDIESMSFEVRKPLSMPPP--PRYYPPPPKPWFPWLMPLTFIANIAMFA 60
           MGK+  P PP  DIE+           PPP  P + PP P PW  WL+PL   AN   FA
Sbjct: 1   MGKR-PPIPP--DIEN---------GPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFA 60

Query: 61  FTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLGPSINILKRYGALDRESVVEHGE 120
            TMY N+CPA +    CL +  LGR SFQP +EN LLGPSI  L++ GAL+R  +VE GE
Sbjct: 61  TTMYVNDCPARSD--ECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERR-LVEEGE 120

Query: 121 GWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIGFLYLLSGFGGSLLSSLNIN 180
            WRLISCIWLH G +HL+ANM+SL+ +G+RLEQEFGF+RIG LY++SG GGSL+S L  +
Sbjct: 121 RWRLISCIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVSCLTDS 180

Query: 181 PVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMMSLILVIALNLAVGFVPHVD 240
             E   +SVGASGALFGLLGAMLSELITNWTIY NKC A+M+LIL+I LNL+VGF+P VD
Sbjct: 181 QGER--VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVD 240

Query: 241 NSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVK-RTAKHQCYQYFLLVLALGLL 300
           NSAH GGF++GF LGFVLL+RPQ+GYVN KYIP G D+K + +KH+CYQ+     +L +L
Sbjct: 241 NSAHFGGFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAIL 286

[Close]

BLAST of MC09g0350 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 320.5 bits (820), Expect = 1.5e-87
Identity = 166/265 (62.64%), Postives = 201/265 (75.85%), Query Frame = 0


Query: 37  PPKPWFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLG 96
           P K WFPWL+P   +ANIA+FA +M+ NNCP  +      F   LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSAYCLARF---LGRFAFQPMKENPLLG 109

Query: 97  PSINILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFL 156
           PS   L++ GALD   VV   E WRL +CIWLHAGV H+LANMLSL+F+GIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 157 RIGFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCA 216
           RIG LY++SGFGGSLLSSL         ISVGASGALFGLLGAMLSEL+TNWTIY+NK A
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 217 AMMSLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAG-SD 276
           A+++LI +IA+NLAVG +PHVDN AH+GGF SGFLLGFV L+RPQ+GY N++  P G + 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 277 VKRTAKHQCYQYFLLVLALGLLIFG 301
               +KH+ YQY L + +L LLI G
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAG 307

[Close]

BLAST of MC09g0350 vs. TAIR 10
Match: AT2G29050.2 (RHOMBOID-like 1 )

HSP 1 Score: 305.8 bits (782), Expect = 3.7e-83
Identity = 166/293 (56.66%), Postives = 201/293 (68.60%), Query Frame = 0


Query: 37  PPKPWFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFYPELGRFSFQPFQENPLLG 96
           P K WFPWL+P   +ANIA+FA +M+ NNCP  +      F   LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSAYCLARF---LGRFAFQPMKENPLLG 109

Query: 97  PSINILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFL 156
           PS   L++ GALD   VV   E WRL +CIWLHAGV H+LANMLSL+F+GIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 157 RIGFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKC- 216
           RIG LY++SGFGGSLLSSL         ISVGASGALFGLLGAMLSEL+TNWTIY+NK  
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKVA 229

Query: 217 ---------------------------AAMMSLILVIALNLAVGFVPHVDNSAHIGGFVS 276
                                      AA+++LI +IA+NLAVG +PHVDN AH+GGF S
Sbjct: 230 KSSLVKQAALSMNDVSIMSLVFLHLQFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTS 289

Query: 277 GFLLGFVLLMRPQFGYVNRKYIPAG-SDVKRTAKHQCYQYFLLVLALGLLIFG 301
           GFLLGFV L+RPQ+GY N++  P G +     +KH+ YQY L + +L LLI G
Sbjct: 290 GFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAG 335

[Close]

BLAST of MC09g0350 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-82
Identity = 154/263 (58.56%), Postives = 200/263 (76.05%), Query Frame = 0


Query: 41  WFPWLMPLTFIANIAMFAFTMYQNNCPAVTGPRRCLFY---PELGRFSFQPFQENPLLGP 100
           W  WL+P+  +AN+A+F   M+ NNCP      R   +     LGR SF+P + NPL GP
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFGP 112

Query: 101 SINILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLR 160
           S + L++ GAL+   VVE  EGWRL++CIWLHAGV+HL ANMLSL+F+GIRLEQ+FGF+R
Sbjct: 113 SSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVR 172

Query: 161 IGFLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAA 220
           IG +YLLSG GGS+LSSL I      +ISVGASGALFGLLG+MLSEL TNWTIYSNK AA
Sbjct: 173 IGVIYLLSGIGGSVLSSLFI----RNSISVGASGALFGLLGSMLSELFTNWTIYSNKIAA 232

Query: 221 MMSLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVK 280
           +++L+ VI +NLA+G +PHVDN AH+GGFV+GFLLGF+LL RPQF ++ R+++P G+ ++
Sbjct: 233 LLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPLR 292

Query: 281 RTAKHQCYQYFLLVLALGLLIFG 301
              K++ YQY L +L+L LLI G
Sbjct: 293 --YKYKTYQYLLWLLSLVLLIAG 309

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BLAST of MC09g0350 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 300.1 bits (767), Expect = 2.1e-81
Identity = 148/262 (56.49%), Postives = 199/262 (75.95%), Query Frame = 0


Query: 41  WFPWLMPLTFIANIAMFAFTMYQNNCP-AVTGPRRCLFYPELGRFSFQPFQENPLLGPSI 100
           W  WL+P   +AN+A+F   M+ N+CP  +TGP +      LGRFSFQP +ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 101 NILKRYGALDRESVVEHGEGWRLISCIWLHAGVVHLLANMLSLLFVGIRLEQEFGFLRIG 160
           + L++ GAL+   VV   +GWRL+SC+WLHAG++HLL NMLSL+F+GIRLEQ+FGF+R+G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 161 FLYLLSGFGGSLLSSLNINPVENPTISVGASGALFGLLGAMLSELITNWTIYSNKCAAMM 220
            +YL+SG GGS+LSSL +      +ISVGASGALFGLLGAMLSEL+TNWTIY+NK AA++
Sbjct: 151 LIYLISGLGGSILSSLFL----QESISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 221 SLILVIALNLAVGFVPHVDNSAHIGGFVSGFLLGFVLLMRPQFGYVNRKYIPAGSDVKRT 280
           +L+ +IA+NLA+G +P VDN AHIGGF++GF LGFVLL+RPQ+G+   +     ++  RT
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR-----TNTSRT 270

Query: 281 -AKHQCYQYFLLVLALGLLIFG 301
             K+  YQY L V+++ LL+ G
Sbjct: 271 KRKYSMYQYVLFVVSVVLLVVG 283

The following BLAST results are available for this feature:

BLAST of MC09g0350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSR0	5.6e-92	59.74	RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1	[more]
Q0WQX7	2.1e-86	62.64	RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1	[more]
Q9LYP1	2.6e-81	58.56	RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1	[more]
Q9CAN1	2.9e-80	56.49	RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1	[more]
F4JBM4	2.3e-77	55.73	RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1	[more]

BLAST of MC09g0350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147001.1	4.55e-219	100.00	RHOMBOID-like protein 5 [Momordica charantia]	[more]
XP_023514894.1	2.58e-187	84.33	RHOMBOID-like protein 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022964405.1	5.21e-187	84.00	RHOMBOID-like protein 5 [Cucurbita moschata] >KAG6593439.1 RHOMBOID-like protein...	[more]
XP_023000373.1	1.74e-185	83.33	RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima]	[more]
XP_011659795.2	5.30e-180	79.33	RHOMBOID-like protein 5 [Cucumis sativus] >KAE8646443.1 hypothetical protein Csa...	[more]

BLAST of MC09g0350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D113	2.20e-219	100.00	RHOMBOID-like protein OS=Momordica charantia OX=3673 GN=LOC111016051 PE=3 SV=1	[more]
A0A6J1HKQ1	2.52e-187	84.00	RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1	[more]
A0A6J1KMF5	8.42e-186	83.33	RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1	[more]
A0A0A0K8T6	1.19e-180	79.33	RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G432610 PE=3 SV=1	[more]
A0A1S3CDZ1	2.91e-179	79.07	RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103499793 PE=3 SV=1	[more]

BLAST of MC09g0350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52580.1	4.0e-93	59.74	RHOMBOID-like protein 5	[more]
AT2G29050.1	1.5e-87	62.64	RHOMBOID-like 1	[more]
AT2G29050.2	3.7e-83	56.66	RHOMBOID-like 1	[more]
AT5G07250.1	1.9e-82	58.56	RHOMBOID-like protein 3	[more]
AT1G63120.1	2.1e-81	56.49	RHOMBOID-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 115..260 e-value: 2.5E-40 score: 137.9
IPR035952	Rhomboid-like superfamily	GENE3D	1.20.1540.10		coord: 42..260 e-value: 5.8E-38 score: 132.4
IPR035952	Rhomboid-like superfamily	SUPERFAMILY	144091	Rhomboid-like	coord: 39..261
IPR002610	Peptidase S54, rhomboid	PANTHER	PTHR22936	RHOMBOID-RELATED	coord: 8..300
None	No IPR available	PANTHER	PTHR22936:SF87	RHOMBOID-LIKE PROTEIN 5	coord: 8..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0350.1	MC09g0350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity