MC09g0325 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0325
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNADH-ubiquinone oxidoreductase chain 4
LocationMC09: 2932647 .. 2932775 (-)
RNA-Seq ExpressionMC09g0325
SyntenyMC09g0325
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGCGGCATATTTGTTTTTCCTTTATACTTTACTCAGATTCGTTTTTATGCTCTTAGCTATTCGGTTGATTCATCTCCAAACAAGAACCATCGATATACAAATATCATTAACCATAAAATTTAGTGAG

mRNA sequence

AAGGCGGCATATTTGTTTTTCCTTTATACTTTACTCAGATTCGTTTTTATGCTCTTAGCTATTCGGTTGATTCATCTCCAAACAAGAACCATCGATATACAAATATCATTAACCATAAAATTTAGTGAG

Coding sequence (CDS)

AAGGCGGCATATTTGTTTTTCCTTTATACTTTACTCAGATTCGTTTTTATGCTCTTAGCTATTCGGTTGATTCATCTCCAAACAAGAACCATCGATATACAAATATCATTAACCATAAAATTTAGTGAG

Protein sequence

KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE
Homology
BLAST of MC09g0325 vs. ExPASy Swiss-Prot
Match: P27572 (NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 9.7e-06
Identity = 32/43 (74.42%), Postives = 34/43 (79.07%), Query Frame = 0

Query: 1   KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 44
           KAAY FFLYTLL  VFMLLAI LI LQT T D+QI LT +FSE
Sbjct: 168 KAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQILLTTEFSE 210

BLAST of MC09g0325 vs. ExPASy Swiss-Prot
Match: Q04050 (NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-05
Identity = 31/43 (72.09%), Postives = 34/43 (79.07%), Query Frame = 0

Query: 1   KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 44
           KAAY FFLYTLL  +FMLLAI LI  QT T D+QISLT +FSE
Sbjct: 168 KAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQISLTTEFSE 210

BLAST of MC09g0325 vs. ExPASy Swiss-Prot
Match: P93313 (NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 6.3e-05
Identity = 30/43 (69.77%), Postives = 33/43 (76.74%), Query Frame = 0

Query: 1   KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 44
           KAAY FFLYTLL  +FMLLAI LI  QT T D+QI LT +FSE
Sbjct: 168 KAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQILLTTEFSE 210

BLAST of MC09g0325 vs. ExPASy Swiss-Prot
Match: P26848 (NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 PE=3 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 1.8e-04
Identity = 28/43 (65.12%), Postives = 32/43 (74.42%), Query Frame = 0

Query: 1   KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 44
           KAAY FFLYTL+  +FMLLAI  I  QT T D+QI LT +FSE
Sbjct: 168 KAAYQFFLYTLMGSLFMLLAILFIFFQTGTTDLQILLTTEFSE 210

BLAST of MC09g0325 vs. NCBI nr
Match: KAB2058092.1 (hypothetical protein ES319_A11G211000v1 [Gossypium barbadense] >TYG94932.1 hypothetical protein ES288_A11G227400v1 [Gossypium darwinii])

HSP 1 Score: 53.9 bits (128), Expect = 6.56e-08
Identity = 33/43 (76.74%), Postives = 36/43 (83.72%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  +FMLLAI LI LQTRT D+QISLT +FSE
Sbjct: 4  KAAYQFFLYTLLGSIFMLLAILLILLQTRTTDLQISLTTEFSE 46

BLAST of MC09g0325 vs. NCBI nr
Match: KAG4175688.1 (hypothetical protein ERO13_A11G199666v2 [Gossypium hirsutum])

HSP 1 Score: 51.2 bits (121), Expect = 7.59e-07
Identity = 32/43 (74.42%), Postives = 35/43 (81.40%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  +FMLLAI LI LQT T D+QISLT +FSE
Sbjct: 4  KAAYQFFLYTLLGSIFMLLAILLILLQTGTTDLQISLTTEFSE 46

BLAST of MC09g0325 vs. NCBI nr
Match: KAG6621657.1 (hypothetical protein I3842_Q007400, partial [Carya illinoinensis])

HSP 1 Score: 51.6 bits (122), Expect = 3.73e-06
Identity = 33/43 (76.74%), Postives = 35/43 (81.40%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  VFMLLAI LI LQT T D+QISLT +FSE
Sbjct: 10 KAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSE 52

BLAST of MC09g0325 vs. NCBI nr
Match: TIV33366.1 (hypothetical protein E5V91_33190, partial [Mesorhizobium sp.])

HSP 1 Score: 50.1 bits (118), Expect = 4.21e-06
Identity = 32/43 (74.42%), Postives = 34/43 (79.07%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  VFMLLAI LI  QT T D+QISLT +FSE
Sbjct: 34 KAAYQFFLYTLLGSVFMLLAILLILFQTGTADLQISLTTEFSE 76

BLAST of MC09g0325 vs. NCBI nr
Match: WP_131796663.1 (hypothetical protein [Candidatus Frankia datiscae])

HSP 1 Score: 51.6 bits (122), Expect = 4.57e-06
Identity = 33/43 (76.74%), Postives = 35/43 (81.40%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  VFMLLAI LI LQT T D+QISLT +FSE
Sbjct: 34 KAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSE 76

BLAST of MC09g0325 vs. ExPASy TrEMBL
Match: A0A5J5TRC2 (Proton_antipo_M domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_A11G211000v1 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.18e-08
Identity = 33/43 (76.74%), Postives = 36/43 (83.72%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  +FMLLAI LI LQTRT D+QISLT +FSE
Sbjct: 4  KAAYQFFLYTLLGSIFMLLAILLILLQTRTTDLQISLTTEFSE 46

BLAST of MC09g0325 vs. ExPASy TrEMBL
Match: A0A5D2EP51 (Proton_antipo_M domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_A11G227400v1 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.18e-08
Identity = 33/43 (76.74%), Postives = 36/43 (83.72%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  +FMLLAI LI LQTRT D+QISLT +FSE
Sbjct: 4  KAAYQFFLYTLLGSIFMLLAILLILLQTRTTDLQISLTTEFSE 46

BLAST of MC09g0325 vs. ExPASy TrEMBL
Match: A0A529UU08 (Proton_antipo_M domain-containing protein (Fragment) OS=Mesorhizobium sp. OX=1871066 GN=E5V91_33190 PE=4 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 2.04e-06
Identity = 32/43 (74.42%), Postives = 34/43 (79.07%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  VFMLLAI LI  QT T D+QISLT +FSE
Sbjct: 34 KAAYQFFLYTLLGSVFMLLAILLILFQTGTADLQISLTTEFSE 76

BLAST of MC09g0325 vs. ExPASy TrEMBL
Match: A0A385CBM6 (NADH-ubiquinone oxidoreductase chain 4 OS=Styphnolobium japonicum OX=3897 GN=nad4 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.29e-06
Identity = 33/43 (76.74%), Postives = 35/43 (81.40%), Query Frame = 0

Query: 1   KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
           KAAY FFLYTLL  VFMLLAI LI LQT T D+QISLT +FSE
Sbjct: 168 KAAYQFFLYTLLGSVFMLLAILLILLQTGTADVQISLTTEFSE 210

BLAST of MC09g0325 vs. ExPASy TrEMBL
Match: A0A5C7GP56 (Proton_antipo_M domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_028290 PE=4 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.46e-06
Identity = 33/43 (76.74%), Postives = 35/43 (81.40%), Query Frame = 0

Query: 1  KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 43
          KAAY FFLYTLL  VFMLLAI LI LQT T D+QISLT +FSE
Sbjct: 51 KAAYQFFLYTLLGSVFMLLAILLILLQTGTTDLQISLTTEFSE 93

BLAST of MC09g0325 vs. TAIR 10
Match: ATMG00580.1 (NADH dehydrogenase subunit 4 )

HSP 1 Score: 49.3 bits (116), Expect = 9.0e-07
Identity = 31/43 (72.09%), Postives = 34/43 (79.07%), Query Frame = 0

Query: 1   KAAYLFFLYTLLRFVFMLLAIRLIHLQTRTIDIQISLTIKFSE 44
           KAAY FFLYTLL  +FMLLAI LI  QT T D+QISLT +FSE
Sbjct: 168 KAAYQFFLYTLLGSLFMLLAILLILFQTGTTDLQISLTTEFSE 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P275729.7e-0674.42NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 ... [more]
Q040501.3e-0572.09NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=... [more]
P933136.3e-0569.77NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE... [more]
P268481.8e-0465.12NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 P... [more]
Match NameE-valueIdentityDescription
KAB2058092.16.56e-0876.74hypothetical protein ES319_A11G211000v1 [Gossypium barbadense] >TYG94932.1 hypot... [more]
KAG4175688.17.59e-0774.42hypothetical protein ERO13_A11G199666v2 [Gossypium hirsutum][more]
KAG6621657.13.73e-0676.74hypothetical protein I3842_Q007400, partial [Carya illinoinensis][more]
TIV33366.14.21e-0674.42hypothetical protein E5V91_33190, partial [Mesorhizobium sp.][more]
WP_131796663.14.57e-0676.74hypothetical protein [Candidatus Frankia datiscae][more]
Match NameE-valueIdentityDescription
A0A5J5TRC23.18e-0876.74Proton_antipo_M domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES3... [more]
A0A5D2EP513.18e-0876.74Proton_antipo_M domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES28... [more]
A0A529UU082.04e-0674.42Proton_antipo_M domain-containing protein (Fragment) OS=Mesorhizobium sp. OX=187... [more]
A0A385CBM62.29e-0676.74NADH-ubiquinone oxidoreductase chain 4 OS=Styphnolobium japonicum OX=3897 GN=nad... [more]
A0A5C7GP562.46e-0676.74Proton_antipo_M domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62... [more]
Match NameE-valueIdentityDescription
ATMG00580.19.0e-0772.09NADH dehydrogenase subunit 4 [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0325.1MC09g0325.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042773 ATP synthesis coupled electron transport
biological_process GO:0015990 electron transport coupled proton transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031966 mitochondrial membrane
cellular_component GO:0009536 plastid
cellular_component GO:0070469 respirasome
molecular_function GO:0008137 NADH dehydrogenase (ubiquinone) activity
molecular_function GO:0048039 ubiquinone binding