MC09g0291 (gene) Bitter gourd (Dali-11) v1

BLAST of MC09g0291 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 5.9e-13
Identity = 42/167 (25.15%), Postives = 92/167 (55.09%), Query Frame = 0

Query: 13  KCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVLKCY 72
           K K  +KL   R+  ++ KK  +++  K ++AELLR   +++A  R E ++ +  +++C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  DLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELRELRSLFTAKYGR 132
            +I+     ++ ++++++  +E P E +E++ +L+Y++ R   +PEL ++++   AKYG+
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 SFEQ--------FTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEW 172
             E           N  ++ K S       +  Q L EIA++  ++W
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of MC09g0291 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.5e-05
Identity = 38/163 (23.31%), Postives = 81/163 (49.69%), Query Frame = 0

Query: 18  VKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVLKCYDLIDE 77
           ++L+  RL  + KKK  + +  + +IA+ L +G D+ A  R E ++ E  +++  ++++ 
Sbjct: 15  LRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILEL 74

Query: 78  FSGTIYNQLSVLSKQSECPDECREAVASLIYAAARF-ADLPELRELRSLFTAKYGRSFEQ 137
           +   +  +  ++    E      E+V++LI+AA R  +++ EL+ +     AKY + + +
Sbjct: 75  YCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGK 134

Query: 138 F--------TNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEW 172
                     N  L+ K S  A  K +  + L EIA+   + +
Sbjct: 135 LCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPY 177

BLAST of MC09g0291 vs. NCBI nr
Match: XP_022146255.1 (vacuolar protein sorting-associated protein IST1 [Momordica charantia])

HSP 1 Score: 843 bits (2179), Expect = 1.12e-307
Identity = 447/449 (99.55%), Postives = 447/449 (99.55%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELRSLFTAKYGRSFEQFTNK LIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240
           YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300
           GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300

Query: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360
           RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY
Sbjct: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360

Query: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNELIVPMK 420
           SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNE IVPMK
Sbjct: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK 420

Query: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           RHTRNNSFVHPKLPDYDQLAARFAALKEK
Sbjct: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 449

BLAST of MC09g0291 vs. NCBI nr
Match: XP_038897244.1 (uncharacterized protein LOC120085370 [Benincasa hispida])

HSP 1 Score: 575 bits (1483), Expect = 1.87e-201
Identity = 327/472 (69.28%), Postives = 378/472 (80.08%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMF KPKF+TKCKSCVK+ K RLD+IRKKK AVLK+LKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDSIRKKKKAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVERNVL+CY LIDEF GTI +Q+ VLSK+SECP+EC+EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYLLIDEFCGTISSQIPVLSKESECPEECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELRSLFT KYG SF  FTNK LIEK  A A TKE+K+QLLQEIAQ+S I+WNSKALEQQL
Sbjct: 121 ELRSLFTEKYGSSFGSFTNKELIEKSRATAQTKEMKIQLLQEIAQDSAIDWNSKALEQQL 180

Query: 181 YTPP-QNTPDEEPNAD-RRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTET 240
           YTPP QN P  E +A  +RNK K+V++PV ERKT   +KKDNSD++SIFDSRSE N TET
Sbjct: 181 YTPPPQNEPAGERSATIKRNKTKIVTVPVHERKTISPRKKDNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE------------ 300
           STGD STDQDV KG+ EDEVED+KPFYLRF+PPPYLK+KP K EA+ E            
Sbjct: 241 STGD-STDQDVHKGISEDEVEDQKPFYLRFVPPPYLKTKPIKTEANVEESQKVTVGNNDE 300

Query: 301 ------KPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMM 360
                 K   EEKPKPRSVRRRN K +P RDINI +  +S  DG EK S S+NKGKETM+
Sbjct: 301 SNKQHPKSPTEEKPKPRSVRRRNVKPQPARDINIDDAGSSTIDGAEKTSLSQNKGKETMI 360

Query: 361 GEEKGAGE-EERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQD--EPQRTKNSKAEE 420
           GEE+GAG+ EER+LDGLLM YS+KK++QESNR KGNLK Q+ +E+D  EPQ+ K++K E 
Sbjct: 361 GEERGAGDDEERVLDGLLMQYSRKKTSQESNRGKGNLKPQRQQEKDNAEPQKPKSTKTE- 420

Query: 421 DLSVPGRSVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           D  VP R+VSLP D +E   P+K+HTR NSFVHPKLP+YDQLAAR AALKEK
Sbjct: 421 DSFVPARAVSLPTDQSE---PVKKHTRTNSFVHPKLPEYDQLAARIAALKEK 467

BLAST of MC09g0291 vs. NCBI nr
Match: KAG6575541.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 555 bits (1431), Expect = 8.68e-194
Identity = 315/465 (67.74%), Postives = 358/465 (76.99%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNK L+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPDEEPNAD-RRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E +    RNK+K+VS+PV+ R T+  +KK+NSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDRERSTSTNRNKSKIVSVPVYGRTTNSPRKKENSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SS RNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++ E D  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRTE-DAYVPTK 420

Query: 421 SVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           S+SLP    E I P+K+H R NS+VHPKLPDYDQLAARFAALKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of MC09g0291 vs. NCBI nr
Match: KAG7014085.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 555 bits (1430), Expect = 1.23e-193
Identity = 315/465 (67.74%), Postives = 358/465 (76.99%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNK L+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPDEEPNAD-RRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E +    R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SS RNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++ E D  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRTE-DAYVPTK 420

Query: 421 SVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           S+SLP    E I P+K+H R NS+VHPKLPDYDQLAARFAALKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of MC09g0291 vs. NCBI nr
Match: XP_022953212.1 (uncharacterized protein LOC111455825 [Cucurbita moschata])

HSP 1 Score: 554 bits (1427), Expect = 3.52e-193
Identity = 315/465 (67.74%), Postives = 359/465 (77.20%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNK L+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SSSRNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++ E D  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRTE-DAYVPTK 420

Query: 421 SVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           S+SLP    E I P+K+H R NS+VHPKLPDYDQLAARFA+LKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of MC09g0291 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 843 bits (2179), Expect = 5.44e-308
Identity = 447/449 (99.55%), Postives = 447/449 (99.55%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELRSLFTAKYGRSFEQFTNK LIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240
           YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300
           GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVR 300

Query: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360
           RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY
Sbjct: 301 RRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKGAGEEERMLDGLLMHY 360

Query: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNELIVPMK 420
           SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNE IVPMK
Sbjct: 361 SKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGRSVSLPRDTNEPIVPMK 420

Query: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           RHTRNNSFVHPKLPDYDQLAARFAALKEK
Sbjct: 421 RHTRNNSFVHPKLPDYDQLAARFAALKEK 449

BLAST of MC09g0291 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 1.70e-193
Identity = 315/465 (67.74%), Postives = 359/465 (77.20%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+  +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNK L+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN  D E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KGV  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
              A+EEKPKPRSVRRR++K +P R INI +   SA DG  K SSSRNKGKE M GEEKG
Sbjct: 301 PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKKK+ QE             K+  EPQR KN++ E D  VP +
Sbjct: 361 VEDDEERMLDGLLKLYSKKKTRQED------------KDNAEPQRPKNTRTE-DAYVPTK 420

Query: 421 SVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           S+SLP    E I P+K+H R NS+VHPKLPDYDQLAARFA+LKEK
Sbjct: 421 SLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of MC09g0291 vs. ExPASy TrEMBL
Match: A0A6J1JV69 (uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629 PE=3 SV=1)

HSP 1 Score: 547 bits (1410), Expect = 6.50e-191
Identity = 311/465 (66.88%), Postives = 355/465 (76.34%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF MFFKPKF+TKCKSCVK+ K RLDTIRKKK  VLKFLKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVE+NVL+CYDLIDEF GTI+N +SVL+K+SECPDEC+EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
            LRSLFT +YG SF  FTNK L+EK  A+A TKE KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNT-PDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240
           Y PPQN    E   +  R+K+K+VS+PV+ R T+  +KKDNSDDDSIFDSRSE N TETS
Sbjct: 181 YVPPQNELVRERSTSTNRSKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVQKGVF-EDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKP---------- 300
            GDSSTDQDV KG+  EDEVED+KPFYLRFI PPYLK+KP+KKE + E+P          
Sbjct: 241 MGDSSTDQDVHKGIVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVTEEKRNN 300

Query: 301 ---AVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEEKG 360
                EEKPKPRSVRRR++K +P R INI +   SA DG  K SS +NKGKE M GEEKG
Sbjct: 301 PKTTTEEKPKPRSVRRRHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAMTGEEKG 360

Query: 361 A-GEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVPGR 420
              +EERMLDGLL  YSKK + QE             K+  EPQR KN++ E D  VP +
Sbjct: 361 EEDDEERMLDGLLKIYSKKNTRQED------------KDNAEPQRPKNTRTE-DAYVPAK 420

Query: 421 SVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
           S+SLP    E I P+K+H R NS+VHPKLPDYDQLAARFAALKEK
Sbjct: 421 SLSLPTARTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of MC09g0291 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 540 bits (1392), Expect = 3.91e-188
Identity = 313/468 (66.88%), Postives = 362/468 (77.35%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMF KPKF+TKCKSCVK+ K RLDTIRKKKNAVLK+LKNDI ELL+S LD NA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQ+ VL+K+SECPDEC+EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELR+LFT KYG SF  FTNK  IEK  A   TKE+K+QLLQEIAQE+ I+WNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YTPP-QNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTET 240
           YTPP QN  D E   A +RNK KVVS+PV+ERK +  + K+NSD++SIFDSRSE N TET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPA--------- 300
           STGD STDQD+ KGV EDEVED+KPF  RF+ PPYLK+KP K EA+ ++P          
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVTIENDES 300

Query: 301 ------VEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEE 360
                  EEKPKPRSVRRRN K +P RDINI +V +S  D T K SSSRNKGKETM+GEE
Sbjct: 301 NNPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETMIGEE 360

Query: 361 KGA-GEEERMLDGLLMHYSKKKSTQES-NRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSV 420
           KGA  +EER+LDGLLM YSKKK+ QES +R K NLK Q+ +E+D  +  + +        
Sbjct: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTT------- 420

Query: 421 PGRSVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
             R+VSLP D NE     K+HTR NSFVHPKLP+YDQLAAR AALKEK
Sbjct: 421 --RAVSLPTDQNE---STKKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of MC09g0291 vs. ExPASy TrEMBL
Match: A0A0A0K926 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446760 PE=3 SV=1)

HSP 1 Score: 526 bits (1355), Expect = 1.56e-182
Identity = 308/468 (65.81%), Postives = 357/468 (76.28%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFDMF KPKF+TKCKSCVK+ K RLDT RKKKNAVLK+LKNDI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTTRKKKNAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQ+ VL+K+SECPDEC+E+VA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKESVATLIYAAARFADLPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELR+LFT KYG SF  FTNK  IEK      TKE+K+QLLQEIAQE+ I+WNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRTTTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YTPP-QNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTET 240
           YTPP +N  D E   A +RNK KVVS+PV+E+K +  + K+NSD++SIFDSRSE N TET
Sbjct: 181 YTPPPENELDGERSGATKRNKTKVVSVPVYEKKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE------------ 300
           STGD STDQDV KGV  DEV D+KPF  RF+ PPYLK+KP K EA+ E            
Sbjct: 241 STGD-STDQDVHKGVSGDEV-DQKPFNRRFVQPPYLKTKPIKTEANAEELPRKVTIENDE 300

Query: 301 ---KPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGEE 360
              K   EEKPKPRSVRRR  K +P RDINI +V +S  D T+K SS RNKGKETM+GEE
Sbjct: 301 SNPKSPTEEKPKPRSVRRRIVKPQPARDINIDDVGSSTVDVTKKISSIRNKGKETMIGEE 360

Query: 361 KGA-GEEERMLDGLLMHYSKKKSTQES-NRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSV 420
           KGA  +EER+LDGLLM YSKKK+ QES +R K NLK Q+ +E+D  +  + +        
Sbjct: 361 KGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPT-------- 420

Query: 421 PGRSVSLPRDTNELIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449
             R+VS P D NE   PMK+HTR NSFVHPKLP+YDQLAAR AALKEK
Sbjct: 421 -SRAVSFPPDQNE---PMKKHTRTNSFVHPKLPEYDQLAARIAALKEK 454

BLAST of MC09g0291 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.7e-63
Identity = 149/326 (45.71%), Postives = 210/326 (64.42%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFD  FKPKF+TKCKS VK+ K R+DT+++KKN+V K+LKNDI +LL++ LD NAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GL+ E+  L CY+ +++F   + + +S+L K   CPDECREA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           +LRSLF  +YG + +QF N   +E+  A   +KE+K++LLQEIA+E  I+W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YTPPQ----NTPDEEPNADRRNKAKVVSIPVFERKTSL-LQKKDNSDDDS--IFDSRSED 240
           YTPP     +T  EEP  ++ N     ++   E+++SL    +++S DDS     S SE+
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTM---EKRSSLSFHGREDSMDDSNKSIMSTSEE 240

Query: 241 NGTETSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFI-PPPYLKSKPSKKEASFEKPAVEE 300
           +   TS G   T         ED+ E  KPFY RF+ P PY K K  K+E+  EK     
Sbjct: 241 DSMSTS-GSCMTGP-------EDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSH 300

Query: 301 ----------KPKPRSVRRRNSKLEP 309
                     KPKPRSVRRR +   P
Sbjct: 301 LMDTESPTGGKPKPRSVRRRFANPPP 314

BLAST of MC09g0291 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 163.3 bits (412), Expect = 4.5e-40
Identity = 111/267 (41.57%), Postives = 161/267 (60.30%), Query Frame = 0

Query: 60  EGLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPEL 119
           EGL+ E+  L CY+ +++F   + + +S+L K   CPDECREA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQ 179
           R+LRSLF  +YG + +QF N   +E+  A   +KE+K++LLQEIA+E  I+W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYTPPQ----NTPDEEPNADRRNKAKVVSIPVFERKTSL-LQKKDNSDDDS--IFDSRSE 239
           LYTPP     +T  EEP  ++ N     ++   E+++SL    +++S DDS     S SE
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTM---EKRSSLSFHGREDSMDDSNKSIMSTSE 225

Query: 240 DNGTETSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFI-PPPYLKSKPSKKEASFEKPAVE 299
           ++   TS G   T         ED+ E  KPFY RF+ P PY K K  K+E+  EK    
Sbjct: 226 EDSMSTS-GSCMTGP-------EDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTS 285

Query: 300 E----------KPKPRSVRRRNSKLEP 309
                      KPKPRSVRRR +   P
Sbjct: 286 HLMDTESPTGGKPKPRSVRRRFANPPP 300

BLAST of MC09g0291 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 146.0 bits (367), Expect = 7.4e-35
Identity = 110/317 (34.70%), Postives = 174/317 (54.89%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MF   FKPKF+ K KS    +K+R+D +R+K+ A+++  K DI   L++G D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
            LL E  ++ CYDLI+ F   I   LS++ K+ ECP+ECREAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAM-AMTKEVKLQLLQEIAQESGIEWNSKALEQQ 180
           +LR++FT ++G       N  L+EK   +   ++E+K+Q ++++A E  I W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LY--TPPQNTPDE-EPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSI------FDSR 240
           L   T      D+ E  AD  NK         + + S +   ++SDD+S+       DS 
Sbjct: 181 LLRETSALQIKDKVETRADYLNK---------DTEKSFMD--EHSDDESVLSESWRIDSL 240

Query: 241 SEDNGTETSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFEKPAV 300
           S+ + + +S+  SS+ +          V  RK    + +P   + S  +K  A  ++ A 
Sbjct: 241 SKGSLSSSSSSSSSSLR---------RVSVRKKKGKKILPYGIISSPDTKLGAGNDEKAQ 297

Query: 301 EEKPKPRSVR-RRNSKL 307
           EEK + R +  + NS+L
Sbjct: 301 EEKEEKRKIADQENSRL 297

BLAST of MC09g0291 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-29
Identity = 136/466 (29.18%), Postives = 211/466 (45.28%), Query Frame = 0

Query: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60
           MFD F    F  K K  +KL K R+D +R+K+NA +KFLK D+A+L+ +G D NA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120
           GLL E   L   D +++    +Y QLS + K  ECP++CREA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRSLFTAKYGRSFEQFTNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180
           ELR +F  KY  S   F N+ L+E  S+   + E K++L++++A E  I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 --------YTPPQNTPDEEPNADRRNK-AKVVSIPVFERKTSLLQKKDNSDD--DSIF-- 240
                      P++T D+    DR     K       E   SL +K   + +  D +F  
Sbjct: 181 VRQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLFQS 240

Query: 241 DSRSEDNGTE------TSTGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKK 300
           D  S  NG        T    S       +   +D   +RK FYL      + K  P++ 
Sbjct: 241 DKESYQNGLRGNQRGLTYKERSENVLHASRSESKDNKAERKEFYL------HSKQNPAR- 300

Query: 301 EASFEKPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDN---------SANDGTEKASSSR 360
                     EK +P      N        +N GN+            A+  TE  +S R
Sbjct: 301 ----------EKHQPIF----NEGDTIVMKVNYGNLGQGNGHRPGVVDAHKKTEFVASER 360

Query: 361 NKGKETMMGEEKGAGEEERMLDG--LLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRT 420
            +       E      +    DG  ++M    +   Q +    G +   K  E +  ++ 
Sbjct: 361 KEFYSQSKQEPSRERHQPIFNDGDTIVMKVKHENHVQGNGHKNGVVDLHKKIEVNASEKL 420

Query: 421 KNSKAEE-DLSVPGRSVSLPRDTNELIVPMKRHTRNNSFVHPKLPD 436
           K+S ++  D  V G          E      +H +N    H K+ D
Sbjct: 421 KSSSSKRADKLVIGFK-------QESFFQGYKHEKNEEHAHQKVED 438

BLAST of MC09g0291 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.3e-25
Identity = 130/484 (26.86%), Postives = 214/484 (44.21%), Query Frame = 0

Query: 12  TKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAEGLLVERNVLKC 71
           +KCK+  K+   R+  IR K+  V+K ++ DIA LL+SG D  A  R E ++ E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELRELRSLFTAKYG 131
            ++I+ F   I ++L++++KQ +CP + +E +ASLI+AA R +++PEL +LR +F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 RSFEQF---------TNKGLIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQLYT 191
           + F             N+ LI+K S      E KL++++EIA+E  ++W++   EQ+L  
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELLK 208

Query: 192 PPQNTPDEEPNADRRNKAKVVSIPV--------------FERKTSLLQKKDNSDDD---- 251
           P + + D       R      S+PV                R TS +    +  D     
Sbjct: 209 PQEESID-----GPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAA 268

Query: 252 -----------------SIFDSRSEDNGTETS-TGDSSTDQDVQKGVFEDEVEDRKPFYL 311
                            S+  +R + +  E S + D ST Q       + +  D    + 
Sbjct: 269 EAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQK------DSQYMDHHHHH- 328

Query: 312 RFIPPPYLKSKPSKKEASFEKPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTE 371
              P    +S+ S+  + + KP  E     R + RR+S           N   + +D  E
Sbjct: 329 ---PGSRRQSRDSETSSYYAKPGAEN----RGMGRRHS---------YNNPGINESDYEE 388

Query: 372 KASSSRNKGKETMMGEEKGAGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDE 431
           + +++  + KETM                    Y+ +     +       +S  ++E+ E
Sbjct: 389 EYTNTEAEAKETMRRRHS---------------YNPRSVPPPATSEIKFDESDYYEEETE 448

Query: 432 PQRTKNSKAEEDLSVPGRSVSLPRDTNELIVPMKRHTRNNSF---VHPKLPDYDQLAARF 448
           P          D    GR  SLP +      P    +R +S    VHPKLPDYD LAARF
Sbjct: 449 P----------DEPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQVHPKLPDYDILAARF 459