MC09g0279 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g0279
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionhydroxyphenylpyruvate reductase-like
LocationMC09: 2525236 .. 2528230 (+)
RNA-Seq ExpressionMC09g0279
SyntenyMC09g0279
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAATTTTTACACGCTGCTCTGAAATGGAGATGGAGAAGATAGGAGTCGCAATGACGACCCCCATGTTCGATTACCTTGAACAACAGCTTGAACAGCGTTTCAAGCTCTTCAAACTCTGGAACCATCCCCTGAACTCCGATTACGTTCGAGAAAACGCGCATTCCATCAGAGCAGTAGTCGGCGGCACCAAGTCCGGCGCCGACGCTAATTTGATACACTCCTTGCCTGCGTTGGAAATCGTTGCGAGCTTCAGCGTTGGTCTGGACAAGATTGATTTGCCAAAATGCCAGGAGAAGGGGATAAGGGTTACCAATACGCCCGACGTATTGACTGACGACGTCGCAGATGCCGCGATTGGACTTGCCATGGCGGTTTCGAGGAGGATTTGTGAGTCTGATCGGTTTGTCCGAAGTGGATCGTGGAAGGAAGGCGGTTTCCGATTGGCGACGAAGGTATGCTGCTTTCATTGCATTACTTTGTGCATTCCGCGCTATCTCTTACCTTAATTTGACTATGGTGAATCCTTGATGATATTGCTTGTTACGATTAAGCTCGACTGCGGGGGGAGGAACCTTAGATACTAGATTGCAGAATGTTTCTGTTAGATTCCATAGATATTAGATTGCAGAATGTTTCTGTTAGATTCCAGATATTTTCATTCATTCGATCTTGATGAACCGCCAAGATTACACTCTATCGCGATCCGACTTCATATCATTTTTCTCATCCTACTCTCTAAACCTTGGGACCAGAAGTATAGTCGAGTTTGAAGATTATAGAATTTTCCCTTACTATGAACTATGGCGAATCTATATTTCTTGGTTTGGTTGATTGACGCAGTAGTTGTTTCTTTCAGTTCAGTGGTAAATCAGTTGGAATTTTGGGGTTAGGAAGGATTGGTTCAGCCATTGCCAAGAGAGTTGAAGCATTTGGTTGCCCCATCAGTTATTGCTCTAGGAATGAAAAGCATCACAGTGGTTACAATTACAAATACTTTTCCAATGCCATAGACTTGGCTGCAAATTCCCAGATTTTGTTTGTGGCATGCACTTTGACTGAAGAAACTAAGCACATTGTCAATCGAGAAGTAATTGATGCACTGGGGCCAAATGGCATTGTTATCAACGTTGGACGAGGTGCTCATGTCAATGAATCTGAGCTTGTCTCAGCATTACTTGAAGGTCGTTTGGCAGGGGCAGGACTCGATGTATTTGAAAACGAGCCCCATGTACCTGAACAGTTGTTTGAGCTTGAAAAAGTAGTTCTTCTGCCTCACGTGGGAACTGATACCATGGAGACCAGCACCGCAATGGCAGACCTTGTAATTAGAAACTTGGAGGCACATTTCAGGAATGAGCCTCTGATTACTCCTGTGATTTGATTTCTTTATACTTTTAAAAATGTCCTGGTAGTCATCGAAACATTTGAGCTAGGGTGTTAATTTAATATTATTATTGTTTTATATGGTTCTCTTGTTTCCCAGAAAGTTTGTTGTTTACACAAGCTTTATGTGGTGAAGGAAAACCATTTCCATTTCTCACTGAAGCTGGCTCCTTCTTCATCAGTAGATTTGTTAGGGATCTGATTTAATAATATGACTCCAATTGAAAAGTTTTGGATATTTTCTGACCATTTCATAAACCAGGAGGATGCTTGTAATTGATTTTCTTTTCCCAGAAAATAGAAATTGAAGAAAAGGGGCAATGAAAATACTTGAGAAGGATTGATAACTGTCTCCCAGGATTTCAAATATTTACAGAGTATACCCACAAAAGCATCTAAAGCTTTTTGAACATAAACATCCAAACAATTCCATATTGTCATTTGGACACTTCCTACCATCTCCCTTATGTTTCTTCAATTCGATCTTTTCCTTTCCTATGAACTGGCATAGCCACAGAAAAGAAATCACTTACTCCCCCCCCCCTCCCTCCCACGCCGCCCACGCAGNNNNNNNCCCCCCCCTTTTTCTTTTGCTTCTCAAAATCTCTAGAGATTCAAATAAAGAGAAAAATACAACTCAAGTCATGGGGCCGAAGAAGTCACCCCTTTGAAGATGAGAAAGTTAAGAGTCTCCAGTTTGAAGTAGATGAAAGACCCTTTGGAGTGGGTAAAGAAACAACCAAAATTTGAACCCACCACACATTGCCATCCGCTTCCATATCTGTTGTCAAAATCCTGCAAAAATCCACAACCAAACCAGTTGAGGAATCAAACACTGAGATTCTGTTACCAAAGTCTTCTCCTCCACACAGACACAGAACCTCAACCAACCAACATGGTTCTGCCATTAAAGTCAAGAAAAAAACATCTGCAACCCACCAAGTTCTTCCTCACATACACGACAAACGAAGATAGAGAGAGAAAGAGAGAGAGGTAACCTTTTTTATGTGGCCAGCAATGGATTTGCAGTCGAAAACATCATAGAGATCCAGAGCTTTATAAGCGGCGGCCATGGCTTGGACCTGCATCTTGCCAGGCATGTCTGTGTCCTGTACCAAAGCTTTGCCTTCCAACATCCTTTCCCTTCGCTGAAAAAAGAAAACTGGTAGGGATTTGCACTCAGCTATCTCTTTTGAACCTTATCTTCAAGACCCAGAAAAAAACAATCCCAATTTCAAACGTTTCTTCTTCCAAGAATATAAACAAAAACAGAACTAAGAAGAAGGAGAAGCAAATGGATGATGATTATATTCAAGGGCAGACTCTGGAAGCCCCTCCTCTTCCAAATGTGATGGAATCTTTTGAAATTCAAGCTCAAGCTTGGCACATGTTCCGATTAATATTGTTTTTCTGTCCTTCTCTCTCCCCACTTTAAGGACTTTTTAACAATATTCTATAAATAAAAAAAATATCTTTCCTCTCTCTCTCTTCCTTTTCCTTTTCCTTTTCCTTTTCCTTTTCCCTGCAATGAAGATAATCATGGCTAAAGGATAAAGCTGCTGAGGCCAATAATGAAGCCAAGTTTTTCGTTTGGTTTTTTCTGTTGATGAG

mRNA sequence

CGAATTTTTACACGCTGCTCTGAAATGGAGATGGAGAAGATAGGAGTCGCAATGACGACCCCCATGTTCGATTACCTTGAACAACAGCTTGAACAGCGTTTCAAGCTCTTCAAACTCTGGAACCATCCCCTGAACTCCGATTACGTTCGAGAAAACGCGCATTCCATCAGAGCAGTAGTCGGCGGCACCAAGTCCGGCGCCGACGCTAATTTGATACACTCCTTGCCTGCGTTGGAAATCGTTGCGAGCTTCAGCGTTGGTCTGGACAAGATTGATTTGCCAAAATGCCAGGAGAAGGGGATAAGGGTTACCAATACGCCCGACGTATTGACTGACGACGTCGCAGATGCCGCGATTGGACTTGCCATGGCGGTTTCGAGGAGGATTTGTGAGTCTGATCGGTTTGTCCGAAGTGGATCGTGGAAGGAAGGCGGTTTCCGATTGGCGACGAAGTTCAGTGGTAAATCAGTTGGAATTTTGGGGTTAGGAAGGATTGGTTCAGCCATTGCCAAGAGAGTTGAAGCATTTGGTTGCCCCATCAGTTATTGCTCTAGGAATGAAAAGCATCACAGTGGTTACAATTACAAATACTTTTCCAATGCCATAGACTTGGCTGCAAATTCCCAGATTTTGTTTGTGGCATGCACTTTGACTGAAGAAACTAAGCACATTGTCAATCGAGAAGTAATTGATGCACTGGGGCCAAATGGCATTGTTATCAACGTTGGACGAGGTGCTCATGTCAATGAATCTGAGCTTGTCTCAGCATTACTTGAAGGTCGTTTGGCAGGGGCAGGACTCGATGTATTTGAAAACGAGCCCCATGTACCTGAACAGTTGTTTGAGCTTGAAAAAGTAGTTCTTCTGCCTCACGTGGGAACTGATACCATGGAGACCAGCACCGCAATGGCAGACCTTGTAATTAGAAACTTGGAGGCACATTTCAGGAATGAGCCTCTGATTACTCCTGTGATTTGATTTCTTTATACTTTTAAAAATGTCCTGGTAGTCATCGAAACATTTGAGCTAGGGTGTTAATTTAATATTATTATTGTTTTATATGGTTCTCTTGTTTCCCAGAAAGTTTGTTGTTTACACAAGCTTTATGTGGTGAAGGAAAACCATTTCCATTTCTCACTGAAGCTGGCTCCTTCTTCATCAGTAGATTTGTTAGGGATCTGATTTAATAATATGACTCCAATTGAAAAGTTTTGGATATTTTCTGACCATTTCATAAACCAGGAGGATGCTTGTAATTGATTTTCTTTTCCCAGAAAATAGAAATTGAAGAAAAGGGGCAATGAAAATACTTGAGAAGGATTGATAACTGTCTCCCAGGATTTCAAATATTTACAGAGTATACCCACAAAAGCATCTAAAGCTTTTTGAACATAAACATCCAAACAATTCCATATTGTCATTTGGACACTTCCTACCATCTCCCTTATGTTTCTTCAATTCGATCTTTTCCTTTCCTATGAACTGGCATAGCCACAGAAAAGAAATCACTTACTCCCCCCCCCCTCCCTCCCACGCCGCCCACGCAGNNNNNNNCCCCCCCCTTTTTCTTTTGCTTCTCAAAATCTCTAGAGATTCAAATAAAGAGAAAAATACAACTCAAGTCATGGGGCCGAAGAAGTCACCCCTTTGAAGATGAGAAAGTTAAGAGTCTCCAGTTTGAAGTAGATGAAAGACCCTTTGGAGTGGGTAAAGAAACAACCAAAATTTGAACCCACCACACATTGCCATCCGCTTCCATATCTGTTGTCAAAATCCTGCAAAAATCCACAACCAAACCAGTTGAGGAATCAAACACTGAGATTCTGTTACCAAAGTCTTCTCCTCCACACAGACACAGAACCTCAACCAACCAACATGGTTCTGCCATTAAAGTCAAGAAAAAAACATCTGCAACCCACCAAGTTCTTCCTCACATACACGACAAACGAAGATAGAGAGAGAAAGAGAGAGAGGTAACCTTTTTTATGTGGCCAGCAATGGATTTGCAGTCGAAAACATCATAGAGATCCAGAGCTTTATAAGCGGCGGCCATGGCTTGGACCTGCATCTTGCCAGGCATGTCTGTGTCCTGTACCAAAGCTTTGCCTTCCAACATCCTTTCCCTTCGCTGAAAAAAGAAAACTGGTAGGGATTTGCACTCAGCTATCTCTTTTGAACCTTATCTTCAAGACCCAGAAAAAAACAATCCCAATTTCAAACGTTTCTTCTTCCAAGAATATAAACAAAAACAGAACTAAGAAGAAGGAGAAGCAAATGGATGATGATTATATTCAAGGGCAGACTCTGGAAGCCCCTCCTCTTCCAAATGTGATGGAATCTTTTGAAATTCAAGCTCAAGCTTGGCACATGTTCCGATTAATATTGTTTTTCTGTCCTTCTCTCTCCCCACTTTAAGGACTTTTTAACAATATTCTATAAATAAAAAAAATATCTTTCCTCTCTCTCTCTTCCTTTTCCTTTTCCTTTTCCTTTTCCTTTTCCCTGCAATGAAGATAATCATGGCTAAAGGATAAAGCTGCTGAGGCCAATAATGAAGCCAAGTTTTTCGTTTGGTTTTTTCTGTTGATGAG

Coding sequence (CDS)

ATGGAGATGGAGAAGATAGGAGTCGCAATGACGACCCCCATGTTCGATTACCTTGAACAACAGCTTGAACAGCGTTTCAAGCTCTTCAAACTCTGGAACCATCCCCTGAACTCCGATTACGTTCGAGAAAACGCGCATTCCATCAGAGCAGTAGTCGGCGGCACCAAGTCCGGCGCCGACGCTAATTTGATACACTCCTTGCCTGCGTTGGAAATCGTTGCGAGCTTCAGCGTTGGTCTGGACAAGATTGATTTGCCAAAATGCCAGGAGAAGGGGATAAGGGTTACCAATACGCCCGACGTATTGACTGACGACGTCGCAGATGCCGCGATTGGACTTGCCATGGCGGTTTCGAGGAGGATTTGTGAGTCTGATCGGTTTGTCCGAAGTGGATCGTGGAAGGAAGGCGGTTTCCGATTGGCGACGAAGTTCAGTGGTAAATCAGTTGGAATTTTGGGGTTAGGAAGGATTGGTTCAGCCATTGCCAAGAGAGTTGAAGCATTTGGTTGCCCCATCAGTTATTGCTCTAGGAATGAAAAGCATCACAGTGGTTACAATTACAAATACTTTTCCAATGCCATAGACTTGGCTGCAAATTCCCAGATTTTGTTTGTGGCATGCACTTTGACTGAAGAAACTAAGCACATTGTCAATCGAGAAGTAATTGATGCACTGGGGCCAAATGGCATTGTTATCAACGTTGGACGAGGTGCTCATGTCAATGAATCTGAGCTTGTCTCAGCATTACTTGAAGGTCGTTTGGCAGGGGCAGGACTCGATGTATTTGAAAACGAGCCCCATGTACCTGAACAGTTGTTTGAGCTTGAAAAAGTAGTTCTTCTGCCTCACGTGGGAACTGATACCATGGAGACCAGCACCGCAATGGCAGACCTTGTAATTAGAAACTTGGAGGCACATTTCAGGAATGAGCCTCTGATTACTCCTGTGATTTGA

Protein sequence

MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRNLEAHFRNEPLITPVI
Homology
BLAST of MC09g0279 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 9.4e-122
Identity = 215/315 (68.25%), Postives = 262/315 (83.17%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGV +T PM  YLEQ+L++RFKLF+ W+ P  +D  RE+++SIRAVVG +  GADA 
Sbjct: 1   MESIGVLLTCPMNPYLEQELDKRFKLFRFWDFPSANDLFREHSNSIRAVVGNSFIGADAQ 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           +I +LP +EIV+SFSVGLDKIDL +C+EKGIRVTNTPDVLT+DVAD A+ L +A  RRIC
Sbjct: 61  MIEALPKMEIVSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRIC 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           ESDR+VRSGSWK+G F+L TKF+GKSVGI+GLGRIGSAIAKR E F CPISY SR EK  
Sbjct: 121 ESDRYVRSGSWKKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEK-- 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
            G NYKY+ + ++LA+N QIL VAC LT ET+HI+NREVI+ALGP G+VIN+GRG HV+E
Sbjct: 181 PGTNYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
            ELVSAL+EGRL GAGLDVFENEP+VPE+L  ++ VVLLPHVG+ T+ET   MADLV+ N
Sbjct: 241 PELVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGN 300

Query: 303 LEAHFRNEPLITPVI 318
           LEAHF N+PL+TPV+
Sbjct: 301 LEAHFLNKPLLTPVV 313

BLAST of MC09g0279 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 427.9 bits (1099), Expect = 9.7e-119
Identity = 211/315 (66.98%), Postives = 254/315 (80.63%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGV M  PM  YLEQ+L++RFKLF+ W  P   D++   A SIRAVVG + +GADA 
Sbjct: 1   MEAIGVLMMCPMSTYLEQELDKRFKLFRYWTQPAQRDFLALQAESIRAVVGNSNAGADAE 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI +LP LEIV+SFSVGLDK+DL KC+EKG+RVTNTPDVLTDDVAD AIGL +AV RRIC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRIC 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           E D++VR G+WK G F+L TKFSGK VGI+GLGRIG A+A+R EAF CPISY SR++K +
Sbjct: 121 ECDKYVRRGAWKFGDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPN 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
           +  NY Y+ + ++LA+NS IL VAC LT ET HI+NREVIDALGP G++IN+GRG HV+E
Sbjct: 181 T--NYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
            ELVSAL+EGRL GAGLDVFE EP VPE+LF LE VVLLPHVG+ T+ET   MADLV+ N
Sbjct: 241 PELVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGN 300

Query: 303 LEAHFRNEPLITPVI 318
           LEAHF  +PL+TPV+
Sbjct: 301 LEAHFSGKPLLTPVV 313

BLAST of MC09g0279 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.5e-114
Identity = 207/315 (65.71%), Postives = 245/315 (77.78%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGV M  PM  YLE +LE+RF L + W  P  S  +  + +SIRAVVG   +GADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI  LP LEIV+SFSVGLDKIDL KC+EKGIRVTNTPDVLT+DVAD AIGL +A+ RR+C
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           E DR+VRSG WK+G F+L TKFSGKSVGI+GLGRIG+AIAKR EAF CPI+Y SR  K  
Sbjct: 121 ECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIK-- 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
               YKY+   +DLA NS IL VAC LTE+T+HIV+R+V+DALG  G++IN+GRG HV+E
Sbjct: 181 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
            EL+ AL EGRL GA LDVFE EPHVPE+LF LE VVLLPHVG+ T+ET  AMADLV+ N
Sbjct: 241 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 300

Query: 303 LEAHFRNEPLITPVI 318
           LEAHF  + L+TPV+
Sbjct: 301 LEAHFSGKSLLTPVV 313

BLAST of MC09g0279 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.4e-71
Identity = 142/307 (46.25%), Postives = 204/307 (66.45%), Query Frame = 0

Query: 13  PMFDYLEQQLEQRFKLFKLWNHPLNS--DYVRENAHSIRAVVGGTKSGADANLIHSLPAL 72
           P   ++++ L + F+          S   +   +A S RA V   +      L+  LP+L
Sbjct: 16  PSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISGRLPVTDELLSHLPSL 75

Query: 73  EIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRS 132
           +I+   SVG+D IDL  C+ +GI +TN  +  +DDVAD A+GL ++V RRI  +DR+VRS
Sbjct: 76  QILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIPAADRYVRS 135

Query: 133 GSW-KEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKY 192
           G+W K G F+L +K SGK VGI+GLG IGS +AKR+E+FGC ISY SR++K  S   Y+Y
Sbjct: 136 GNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQSS--PYRY 195

Query: 193 FSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSAL 252
           +S+ + LA N+ +L + C+LT+ET HIVNREV++ LG +G+VINVGRG  ++E E+V  L
Sbjct: 196 YSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCL 255

Query: 253 LEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRNLEAHFRN 312
           ++G + GAGLDVFENEP VP++LF L+ VVL PH    T  +   +A + + NL+A F N
Sbjct: 256 VDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALANLKAFFSN 315

Query: 313 EPLITPV 317
            PL++PV
Sbjct: 316 RPLLSPV 320

BLAST of MC09g0279 vs. ExPASy Swiss-Prot
Match: Q9C4M5 (Glyoxylate reductase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) OX=523849 GN=gyaR PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-54
Identity = 125/299 (41.81%), Postives = 173/299 (57.86%), Query Frame = 0

Query: 24  QRFKLFKLWNHPL--NSDYVRENAHSIRAVVGGTKSGADANLIHSLPALEIVASFSVGLD 83
           ++F   +LW  P       + E    + A+V       D  L+ + P L+I+A ++VG D
Sbjct: 20  EKFYEIELWKDPKAPPRGVLLEKVREVDALVTLVTDKVDKELLENAPKLKIIAQYAVGYD 79

Query: 84  KIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSGSWK--EGGFR 143
            ID+ +  ++GI VTNTP VLTD  AD A  L +AV+RRI E+D FVRSG WK  E G+ 
Sbjct: 80  NIDIEEATKRGIYVTNTPGVLTDATADLAFALLLAVARRIVEADAFVRSGEWKKSEVGWH 139

Query: 144 ----LATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHS----GYNYKYFS 203
               L     GK++GI+G GRIG A+AKR + FG  I Y SR  K  +    G  Y  F 
Sbjct: 140 PLMFLGYGLKGKTLGIVGFGRIGQALAKRAKGFGMKIIYYSRTRKPEAEEEIGAEYVDFE 199

Query: 204 NAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSALLE 263
               L   S  + +   LT+ET H++  + +  + PN I+IN  RGA V+ + L+ AL E
Sbjct: 200 T---LLKESDFISLHVPLTKETYHMIGEKELKLMKPNAILINTSRGAVVDTNALIKALKE 259

Query: 264 GRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRNLEAHFRNE 311
           G +AGAGLDVFE EP+  E+LF+L+ VVL PH+G+ T E    MA+LV +NL A  + E
Sbjct: 260 GWIAGAGLDVFEEEPYYNEELFKLKNVVLAPHIGSATHEAREGMAELVAKNLIAFAKGE 315

BLAST of MC09g0279 vs. NCBI nr
Match: XP_022155087.1 (uncharacterized protein LOC111022216 [Momordica charantia])

HSP 1 Score: 626 bits (1615), Expect = 5.09e-221
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD
Sbjct: 332 MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 391

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR
Sbjct: 392 ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 451

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK
Sbjct: 452 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 511

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV
Sbjct: 512 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 571

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI
Sbjct: 572 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 631

Query: 301 RNLEAHFRNEPLITPVI 317
           RNLEAHFRNEPLITPVI
Sbjct: 632 RNLEAHFRNEPLITPVI 648

BLAST of MC09g0279 vs. NCBI nr
Match: XP_038897872.1 (glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR-like [Benincasa hispida])

HSP 1 Score: 533 bits (1374), Expect = 1.61e-189
Identity = 271/315 (86.03%), Postives = 289/315 (91.75%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGVAMTTPMF YLEQQLE+RF LFKLWNHPL+SD+++ENA SIRAVVG TKSGADAN
Sbjct: 1   MESIGVAMTTPMFSYLEQQLERRFNLFKLWNHPLDSDFLQENAQSIRAVVGCTKSGADAN 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI SLPALEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAVSRRIC
Sbjct: 61  LIDSLPALEIVATFSVGLDKIDLPKCMEKGIRVVNTPDVLTDDVADAAIGLAMAVSRRIC 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           ESDRFVRSGSW + GFRLAT+F+GKSVGILGLGRIGSAIAKR EAFGCPI Y +R + H 
Sbjct: 121 ESDRFVRSGSWTKDGFRLATRFNGKSVGILGLGRIGSAIAKRAEAFGCPIGYFTRRKMHQ 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
           SGY  KYF + +DLAANSQILFV+CTLTEETKHIVNREVID LGPNGI+INVGRGAH++E
Sbjct: 181 SGY--KYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDTLGPNGILINVGRGAHIDE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
           SELVSALLEGRL GAGLDVFENEPHVPEQL ELE VVLLPHVGTDT+ETS AMADLVI N
Sbjct: 241 SELVSALLEGRLGGAGLDVFENEPHVPEQLLELENVVLLPHVGTDTIETSIAMADLVISN 300

Query: 303 LEAHFRNEPLITPVI 317
           LEAHFRNEPLITPVI
Sbjct: 301 LEAHFRNEPLITPVI 313

BLAST of MC09g0279 vs. NCBI nr
Match: KAG6593464.1 (Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532 bits (1371), Expect = 8.26e-189
Identity = 268/317 (84.54%), Postives = 288/317 (90.85%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           ++MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GAD
Sbjct: 15  LKMEKIGVAMTTPMFSYLEKQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGAD 74

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           ANLI + PALEIVASFSVGLDKIDLPKC+EKGIRVTNTPDVLTDDVADAAIGLA+AVSRR
Sbjct: 75  ANLIDTFPALEIVASFSVGLDKIDLPKCREKGIRVTNTPDVLTDDVADAAIGLAIAVSRR 134

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           ICESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEK
Sbjct: 135 ICESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEK 194

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGY  KYF + +DLAANSQ LFVAC LT+ETKHI+NREVID LGPNGI+INVGRG HV
Sbjct: 195 HHSGY--KYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDGLGPNGILINVGRGVHV 254

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSALLEGRL G GLDVFENEPHVPEQL ELE  VLLPHVGTDT+ET+ AMADLVI
Sbjct: 255 NESELVSALLEGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVI 314

Query: 301 RNLEAHFRNEPLITPVI 317
            NLEAHFRNEPLITPVI
Sbjct: 315 GNLEAHFRNEPLITPVI 329

BLAST of MC09g0279 vs. NCBI nr
Match: XP_023514517.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111778775 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531 bits (1369), Expect = 8.18e-184
Identity = 268/317 (84.54%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           ++MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GAD
Sbjct: 326 LKMEKIGVAMTTPMFSYLEEQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGAD 385

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           ANLI + PALEIVASFSVGLDKIDLPKC+EKGIRVTNTPDVLTDDVADAAIGLA+AVSRR
Sbjct: 386 ANLIDTFPALEIVASFSVGLDKIDLPKCREKGIRVTNTPDVLTDDVADAAIGLAIAVSRR 445

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           ICESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEK
Sbjct: 446 ICESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEK 505

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGY  KYF + +DLAANSQ LFVAC LT+ETKHI+NREVIDALGPNGI+INVGRGAHV
Sbjct: 506 HHSGY--KYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDALGPNGILINVGRGAHV 565

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSAL +GRL G GLDVFENEPHVPEQL ELE  VLLPHVGTDT+ET+ AMADLVI
Sbjct: 566 NESELVSALHKGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVI 625

Query: 301 RNLEAHFRNEPLITPVI 317
            NLEAHFRNEPLITPVI
Sbjct: 626 GNLEAHFRNEPLITPVI 640

BLAST of MC09g0279 vs. NCBI nr
Match: XP_023000640.1 (uncharacterized protein LOC111494888 [Cucurbita maxima])

HSP 1 Score: 530 bits (1365), Expect = 6.74e-183
Identity = 267/317 (84.23%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           ++MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GAD
Sbjct: 348 LKMEKIGVAMTTPMFTYLEKQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGAD 407

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           ANLI + PALEIVASFSVG+DKIDLPKC+ KGIRVTNTP+VLTDDVADAAIGLA+AVSRR
Sbjct: 408 ANLIDTFPALEIVASFSVGIDKIDLPKCRVKGIRVTNTPEVLTDDVADAAIGLAIAVSRR 467

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           ICESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEK
Sbjct: 468 ICESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEK 527

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGY  KYF + +DLAANSQ LFVAC LT+ETKHI+NREVIDALGPNGI+INVGRGAHV
Sbjct: 528 HHSGY--KYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDALGPNGILINVGRGAHV 587

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSALLEGRL G GLDVFENEPHVPEQL ELE  VLLPHVGTDT+ET+ AMADLVI
Sbjct: 588 NESELVSALLEGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVI 647

Query: 301 RNLEAHFRNEPLITPVI 317
            NLEAHFRNEPLITPVI
Sbjct: 648 GNLEAHFRNEPLITPVI 662

BLAST of MC09g0279 vs. ExPASy TrEMBL
Match: A0A6J1DNF0 (uncharacterized protein LOC111022216 OS=Momordica charantia OX=3673 GN=LOC111022216 PE=4 SV=1)

HSP 1 Score: 626 bits (1615), Expect = 2.47e-221
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD
Sbjct: 332 MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 391

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR
Sbjct: 392 ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 451

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK
Sbjct: 452 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 511

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV
Sbjct: 512 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 571

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI
Sbjct: 572 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 631

Query: 301 RNLEAHFRNEPLITPVI 317
           RNLEAHFRNEPLITPVI
Sbjct: 632 RNLEAHFRNEPLITPVI 648

BLAST of MC09g0279 vs. ExPASy TrEMBL
Match: A0A6J1KIX6 (uncharacterized protein LOC111494888 OS=Cucurbita maxima OX=3661 GN=LOC111494888 PE=4 SV=1)

HSP 1 Score: 530 bits (1365), Expect = 3.26e-183
Identity = 267/317 (84.23%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           ++MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GAD
Sbjct: 348 LKMEKIGVAMTTPMFTYLEKQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGAD 407

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           ANLI + PALEIVASFSVG+DKIDLPKC+ KGIRVTNTP+VLTDDVADAAIGLA+AVSRR
Sbjct: 408 ANLIDTFPALEIVASFSVGIDKIDLPKCRVKGIRVTNTPEVLTDDVADAAIGLAIAVSRR 467

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           ICESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEK
Sbjct: 468 ICESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEK 527

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGY  KYF + +DLAANSQ LFVAC LT+ETKHI+NREVIDALGPNGI+INVGRGAHV
Sbjct: 528 HHSGY--KYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDALGPNGILINVGRGAHV 587

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSALLEGRL G GLDVFENEPHVPEQL ELE  VLLPHVGTDT+ET+ AMADLVI
Sbjct: 588 NESELVSALLEGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVI 647

Query: 301 RNLEAHFRNEPLITPVI 317
            NLEAHFRNEPLITPVI
Sbjct: 648 GNLEAHFRNEPLITPVI 662

BLAST of MC09g0279 vs. ExPASy TrEMBL
Match: A0A5D3BWA0 (Hydroxyphenylpyruvate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002220 PE=3 SV=1)

HSP 1 Score: 511 bits (1317), Expect = 3.74e-181
Identity = 264/315 (83.81%), Postives = 281/315 (89.21%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGVAMTTPMF YLEQ LE+RF LFKLWNHPL+SD+++ NA SIRAVVG T SGADA+
Sbjct: 1   MESIGVAMTTPMFGYLEQNLERRFNLFKLWNHPLDSDFLQHNAQSIRAVVGCTTSGADAH 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI + P LEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAV RRI 
Sbjct: 61  LIDTFPNLEIVATFSVGLDKIDLPKCLEKGIRVVNTPDVLTDDVADAAIGLAMAVLRRIS 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           ESDRFVRSGSW +  F LAT+F+GKSVGILGLGRIGSAIAKR EAFGC I Y SR EKHH
Sbjct: 121 ESDRFVRSGSWMKDEFGLATRFNGKSVGILGLGRIGSAIAKRAEAFGCNIGYFSRTEKHH 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
           SGY  KYF + +DLAANSQILFV+CTLTEETKHIVNREVIDALGPNGI+INVGRGAHVNE
Sbjct: 181 SGY--KYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
           SELVSALLE RL GAGLDVFENEPHVPE+L EL  VVLLPHVGTDT+ETS AMADLVIRN
Sbjct: 241 SELVSALLERRLGGAGLDVFENEPHVPEKLLELNNVVLLPHVGTDTIETSIAMADLVIRN 300

Query: 303 LEAHFRNEPLITPVI 317
           LEAHFRNEPLITPVI
Sbjct: 301 LEAHFRNEPLITPVI 313

BLAST of MC09g0279 vs. ExPASy TrEMBL
Match: A0A1S3CG96 (hydroxyphenylpyruvate reductase-like OS=Cucumis melo OX=3656 GN=LOC103500587 PE=3 SV=1)

HSP 1 Score: 512 bits (1318), Expect = 5.86e-181
Identity = 264/317 (83.28%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 1   MEMEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGAD 60
           + ME IGVAMTTPMF YLEQ LE+RF LFKLWNHPL+SD+++ NA SIRAVVG T SGAD
Sbjct: 21  LRMESIGVAMTTPMFGYLEQNLERRFNLFKLWNHPLDSDFLQHNAQSIRAVVGCTTSGAD 80

Query: 61  ANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRR 120
           A+LI + P LEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAV RR
Sbjct: 81  AHLIDTFPNLEIVATFSVGLDKIDLPKCLEKGIRVVNTPDVLTDDVADAAIGLAMAVLRR 140

Query: 121 ICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEK 180
           I ESDRFVRSGSW +  F LAT+F+GKSVGILGLGRIGSAIAKR EAFGC I Y SR EK
Sbjct: 141 ISESDRFVRSGSWMKDEFGLATRFNGKSVGILGLGRIGSAIAKRAEAFGCNIGYFSRTEK 200

Query: 181 HHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHV 240
           HHSGY  KYF + +DLAANSQILFV+CTLTEETKHIVNREVIDALGPNGI+INVGRGAHV
Sbjct: 201 HHSGY--KYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHV 260

Query: 241 NESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVI 300
           NESELVSALLE RL GAGLDVFENEPHVPE+L EL  VVLLPHVGTDT+ETS AMADLVI
Sbjct: 261 NESELVSALLERRLGGAGLDVFENEPHVPEKLLELNNVVLLPHVGTDTIETSIAMADLVI 320

Query: 301 RNLEAHFRNEPLITPVI 317
           RNLEAHFRNEPLITPVI
Sbjct: 321 RNLEAHFRNEPLITPVI 335

BLAST of MC09g0279 vs. ExPASy TrEMBL
Match: A0A0A0K6M4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446950 PE=3 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 1.02e-178
Identity = 260/315 (82.54%), Postives = 277/315 (87.94%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGVAMTT MF YLEQ LE RF LFKLWNHPL+SD+++ NA S+RAVVG TKSGADA 
Sbjct: 1   MESIGVAMTTQMFTYLEQNLEGRFNLFKLWNHPLDSDFLQLNAQSVRAVVGCTKSGADAR 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI + P LEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAV RRI 
Sbjct: 61  LIDTFPNLEIVATFSVGLDKIDLPKCLEKGIRVVNTPDVLTDDVADAAIGLAMAVLRRIS 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           ESDRFVRSGSW +  F L T+F+GKSVGI+GLGRIGSAIAKR  AFGCPI Y SR EKHH
Sbjct: 121 ESDRFVRSGSWMKNDFGLGTRFNGKSVGIIGLGRIGSAIAKRAVAFGCPIGYFSRTEKHH 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
            GY  KYF + +DLAANSQILFV+CTLTEETKHIVNREVIDALGPNGI+INVGRGAHVNE
Sbjct: 181 RGY--KYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
           +ELVSALLE RL GAGLDVFENEPHVPEQL EL  VVLLPHVGTDT+ETS AMADLVIRN
Sbjct: 241 TELVSALLERRLGGAGLDVFENEPHVPEQLLELNNVVLLPHVGTDTIETSIAMADLVIRN 300

Query: 303 LEAHFRNEPLITPVI 317
           LEAHFRNEPLITPVI
Sbjct: 301 LEAHFRNEPLITPVI 313

BLAST of MC09g0279 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.8e-115
Identity = 207/315 (65.71%), Postives = 245/315 (77.78%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGV M  PM  YLE +LE+RF L + W  P  S  +  + +SIRAVVG   +GADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI  LP LEIV+SFSVGLDKIDL KC+EKGIRVTNTPDVLT+DVAD AIGL +A+ RR+C
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           E DR+VRSG WK+G F+L TKFSGKSVGI+GLGRIG+AIAKR EAF CPI+Y SR  K  
Sbjct: 121 ECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIK-- 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
               YKY+   +DLA NS IL VAC LTE+T+HIV+R+V+DALG  G++IN+GRG HV+E
Sbjct: 181 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
            EL+ AL EGRL GA LDVFE EPHVPE+LF LE VVLLPHVG+ T+ET  AMADLV+ N
Sbjct: 241 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 300

Query: 303 LEAHFRNEPLITPVI 318
           LEAHF  + L+TPV+
Sbjct: 301 LEAHFSGKSLLTPVV 313

BLAST of MC09g0279 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.0e-103
Identity = 192/315 (60.95%), Postives = 228/315 (72.38%), Query Frame = 0

Query: 3   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 62
           ME IGV M  PM  YLE +LE+RF L + W  P  S  +  + +SIRAVVG   +GADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 63  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 122
           LI  LP LEIV+SFSVGLDKIDL KC+EKGIRVTNTPDVLT+DVAD AIGL +A+ RR+C
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 123 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 182
           E DR+VRSG WK+                   GRIG+AIAKR EAF CPI+Y SR  K  
Sbjct: 121 ECDRYVRSGKWKQ-------------------GRIGTAIAKRAEAFSCPINYYSRTIK-- 180

Query: 183 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 242
               YKY+   +DLA NS IL VAC LTE+T+HIV+R+V+DALG  G++IN+GRG HV+E
Sbjct: 181 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 240

Query: 243 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 302
            EL+ AL EGRL GA LDVFE EPHVPE+LF LE VVLLPHVG+ T+ET  AMADLV+ N
Sbjct: 241 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 294

Query: 303 LEAHFRNEPLITPVI 318
           LEAHF  + L+TPV+
Sbjct: 301 LEAHFSGKSLLTPVV 294

BLAST of MC09g0279 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.1e-72
Identity = 142/307 (46.25%), Postives = 204/307 (66.45%), Query Frame = 0

Query: 13  PMFDYLEQQLEQRFKLFKLWNHPLNS--DYVRENAHSIRAVVGGTKSGADANLIHSLPAL 72
           P   ++++ L + F+          S   +   +A S RA V   +      L+  LP+L
Sbjct: 16  PSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISGRLPVTDELLSHLPSL 75

Query: 73  EIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRS 132
           +I+   SVG+D IDL  C+ +GI +TN  +  +DDVAD A+GL ++V RRI  +DR+VRS
Sbjct: 76  QILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIPAADRYVRS 135

Query: 133 GSW-KEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKY 192
           G+W K G F+L +K SGK VGI+GLG IGS +AKR+E+FGC ISY SR++K  S   Y+Y
Sbjct: 136 GNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQSS--PYRY 195

Query: 193 FSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSAL 252
           +S+ + LA N+ +L + C+LT+ET HIVNREV++ LG +G+VINVGRG  ++E E+V  L
Sbjct: 196 YSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCL 255

Query: 253 LEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRNLEAHFRN 312
           ++G + GAGLDVFENEP VP++LF L+ VVL PH    T  +   +A + + NL+A F N
Sbjct: 256 VDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALANLKAFFSN 315

Query: 313 EPLITPV 317
            PL++PV
Sbjct: 316 RPLLSPV 320

BLAST of MC09g0279 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.6e-66
Identity = 125/295 (42.37%), Postives = 192/295 (65.08%), Query Frame = 0

Query: 25  RFKLFKLWNHPLN-SDYVRENAHSIRAVVGGTKSGADANLIHSLPALEIVASFSVGLDKI 84
           +F++ K +  PL   +++  ++ SI A++    +   A+LI  LP L +V + S G+D +
Sbjct: 45  KFEILKAFESPLPLPEFLAYHSDSISAIIAPVAAPVTADLIRILPNLRLVVTTSAGVDHV 104

Query: 85  DLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSGSWK-EGGFRLAT 144
           DL +C+ +GI V N     ++DVAD A+GL + V RRI  ++RFV+   W  +G + L +
Sbjct: 105 DLVECRRRGISVANAGSSFSEDVADTAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGS 164

Query: 145 KFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKYFSNAIDLAANSQI 204
           K   K +GI+GLG IGS +A R++AFGC ISY SRN K +    Y Y+ +  ++AANS  
Sbjct: 165 KLGRKRIGIVGLGSIGSKVATRLDAFGCQISYSSRNRKPYD-VPYHYYMDIEEMAANSDA 224

Query: 205 LFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSALLEGRLAGAGLDVF 264
           L + C L E+T  ++N++V+ ALG  G+++NV RGA ++E E+V  L EG + GAGLDVF
Sbjct: 225 LIICCELNEKTLRLINKDVLSALGKRGVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVF 284

Query: 265 ENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRNLEAHFRNEPLITPVI 318
           E+EP+VP++LFEL+ VV  PH    T+E    +  +V+ N+EA F N+PL+TPV+
Sbjct: 285 EDEPNVPKELFELDNVVFSPHSAFMTLEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

BLAST of MC09g0279 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 122.5 bits (306), Expect = 6.2e-28
Identity = 79/260 (30.38%), Postives = 133/260 (51.15%), Query Frame = 0

Query: 74  ASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSG-- 133
           ++ +VG + +D+    + GI V NTP VLT+  A+ A  L++A +RRI E+D F+R G  
Sbjct: 91  SNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLY 150

Query: 134 -SWKEGGFRLATKFSGKSVGILGLGRIGSAIAK-RVEAFGCPISY-----CSRNEKHHSG 193
             W    F +     G++VG++G GRIGSA A+  VE F   + Y      +R EK  + 
Sbjct: 151 EGWLPHLF-VGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTA 210

Query: 194 Y------------NYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVI 253
           Y             +K  S+  ++   + ++ +   L + T H+VN+E +  +    I++
Sbjct: 211 YGQFLKANGEQPVTWKRASSMEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKEAILV 270

Query: 254 NVGRGAHVNESELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETS 313
           N  RG  ++E+ LV  L E  +   GLDVFE EP +   L + +  +++PH+ + +  T 
Sbjct: 271 NCSRGPVIDEAALVEHLKENPMFRVGLDVFEEEPFMKPGLADTKNAIVVPHIASASKWTR 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A5CAL19.4e-12268.25Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN... [more]
Q65CJ79.7e-11966.98Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ... [more]
Q9CA902.5e-11465.71Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q9LE334.4e-7146.25Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR... [more]
Q9C4M54.9e-5441.81Glyoxylate reductase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / J... [more]
Match NameE-valueIdentityDescription
XP_022155087.15.09e-221100.00uncharacterized protein LOC111022216 [Momordica charantia][more]
XP_038897872.11.61e-18986.03glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR-like [Benincasa hispida][more]
KAG6593464.18.26e-18984.54Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023514517.18.18e-18484.54LOW QUALITY PROTEIN: uncharacterized protein LOC111778775 [Cucurbita pepo subsp.... [more]
XP_023000640.16.74e-18384.23uncharacterized protein LOC111494888 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DNF02.47e-221100.00uncharacterized protein LOC111022216 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1KIX63.26e-18384.23uncharacterized protein LOC111494888 OS=Cucurbita maxima OX=3661 GN=LOC111494888... [more]
A0A5D3BWA03.74e-18183.81Hydroxyphenylpyruvate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CG965.86e-18183.28hydroxyphenylpyruvate reductase-like OS=Cucumis melo OX=3656 GN=LOC103500587 PE=... [more]
A0A0A0K6M41.02e-17882.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446950 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G79870.11.8e-11565.71D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.22.0e-10360.95D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G12550.13.1e-7246.25D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT2G45630.22.6e-6642.37D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G68010.16.2e-2830.38hydroxypyruvate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 111..285
e-value: 1.5E-49
score: 167.8
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 16..316
e-value: 3.1E-21
score: 75.8
NoneNo IPR availableGENE3D3.40.50.720coord: 103..288
e-value: 1.4E-122
score: 410.0
NoneNo IPR availableGENE3D3.40.50.720coord: 19..311
e-value: 1.4E-122
score: 410.0
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 3..317
NoneNo IPR availablePANTHERPTHR10996:SF235D-GLYCERATE DEHYDROGENASE/HYDROXYPYRUVATE REDUCTASEcoord: 3..317
NoneNo IPR availableCDDcd12156HPPRcoord: 6..308
e-value: 1.00627E-160
score: 448.456
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 6..138
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 103..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g0279.1MC09g0279.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0030267 glyoxylate reductase (NADP+) activity
molecular_function GO:0016618 hydroxypyruvate reductase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor