MC09g0207 (gene) Bitter gourd (Dali-11) v1

BLAST of MC09g0207 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 2.6e-223
Identity = 377/581 (64.89%), Postives = 457/581 (78.66%), Query Frame = 0

Query: 2   ASFSLWFFSFLSVFLCHGVFKLEASHHVYR--AQTSASVPPDHQQPYRTSYHFQPPKNWM 61
           ++  LW    L++ + + V  LEASHHVY+   Q++ +  P   QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWL--LLTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  ---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGC 121
              NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AIFPS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNN 181
           WSGSATILP  KP +LYTGI+P+N QVQN+A PKNLSDP+L EW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGM 241
           IN+SSFRDPTTAWLG+D++WRV+IGSKI+ RGLAI Y SKDF++W++   PLHY D +GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKNSRVGVDTSVNGPR---VKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPD 301
           WECPDFFPV +    GV+TS  G     +KHVLK+SLDDTKHD+YT+GTY+  KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRS 361
            G        RYDYGK YASKTFFDS K RR+LWGW NESSSV+DD +KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDASGKQLVQWPIVEIQKLRKDKVK-LINKVLKKGSVIEVKGVTAAQVDVEVSFKVKN 421
           IWLD SGKQL+QWP+ E+++LR  +VK L NKVLK GS +EV GVTAAQ DVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFK---REG 481
            +KA V++P W DPQL+C++   + +SG+GPFGL++LASK+L+EYTSV+F++FK      
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLMCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACIT 541
           KYVVLMCSDQSRSSL  +NDKTTYGAFVD++P  + LSLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
           +RVYP LAIG++  L+AFN   + V +  L AWS+N A I+
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of MC09g0207 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 3.1e-208
Identity = 350/542 (64.58%), Postives = 420/542 (77.49%), Query Frame = 0

Query: 12  LSVFLCHGVFKLEASHHVYRAQTSAS---VPPDHQQPYRTSYHFQPPKNWM---NGPMIY 71
           LS+F    V  +EA+HHVY+   + S       + QPYRT+YHFQP KNW+   NGPM Y
Sbjct: 9   LSLFSLIYVIPIEATHHVYQTLETLSSHHSSKSNHQPYRTAYHFQPLKNWINDPNGPMRY 68

Query: 72  KGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCWSGSATILP 131
            G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P  HAI PSQPSDI GCWSGSATILP
Sbjct: 69  GGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDIKGCWSGSATILP 128

Query: 132 GNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNINSSSFRDP 191
           G KPA+LYTGI+P NHQVQN+A+PKN+SDP L EW KSPKNPLM PT  N INSSSFRDP
Sbjct: 129 GGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTIANKINSSSFRDP 188

Query: 192 TTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMWECPDFFPV 251
           TT+WLG+D  WRV+IGSKI+ +G+AI+Y+SK+F+ W +  +PLH A+ TGMWECPDF+PV
Sbjct: 189 TTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEGTGMWECPDFYPV 248

Query: 252 AKNS--RVGVDTSVNG-PRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSVEGYAG 311
              +  R GVDTS NG   V+HVLKVSLDDTKHDHY +G+Y+V KDV+VP+ G  +    
Sbjct: 249 LDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDVFVPENGFEDNGFV 308

Query: 312 LRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLDASGKQ 371
           LRYDYGK YASKTFFD  K RR+L GWVNESSSV DD KKGWSG+  IPR IWL  SGKQ
Sbjct: 309 LRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHTIPREIWLHESGKQ 368

Query: 372 LVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQVLKPE 431
           LVQWP+ EI+ LR + V    KV+K G  I + GV + Q DVE+SF++ +  K + L+ +
Sbjct: 369 LVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFEISDLGKVESLR-K 428

Query: 432 WKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCSDQSRS 491
           W DPQLLC+QKGA  + GVGPFGLL+ AS+ LKEYT+VFF++FK + K +VLMCSDQSRS
Sbjct: 429 WIDPQLLCSQKGAGVKGGVGPFGLLVFASQGLKEYTAVFFRIFKYQDKNLVLMCSDQSRS 488

Query: 492 SLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLAIGENV 545
           SLN +ND T+YG FVD+DP+ E LSLR+LIDHS+VESFG +G+AC+TARVYPTLAI +  
Sbjct: 489 SLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVTARVYPTLAIHDKA 548

BLAST of MC09g0207 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 711.4 bits (1835), Expect = 7.9e-204
Identity = 350/571 (61.30%), Postives = 418/571 (73.20%), Query Frame = 0

Query: 12  LSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWM---NGPMIYKGI 71
           LS+FL + +  LEAS H            D  QPYRT YHFQP KNWM   NGPMIYKGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCWSGSATILPGN 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNINSSSFRDPTT 191
           KP +LYTGI+    QVQN+AVP N+SDP+L EW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGRDRQWRVVIGSKI-NARGLAIMYRSKDFIQWDQVNNPLHYADDTGMWECPDFFPVA 251
           AWLGRD +WRV++GS   + RGLAI+Y+S+DF  W Q   PLHY D TGMWECPDFFPV+
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KNSRVGVDTSVNGPR-VKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSVEGYAGLRY 311
                GV+TS  G   +KHVLKVSL +T HD+YT+G+Y+ +KDVYVPD G V+  +  R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLDASGKQLVQ 371
           DYGK YASKTF+D  KKRR+LWGWVNESS   DD +KGWSG+Q+ PR IWLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQVLKPEWKD 431
           WPI EI+ LR  +V    KVLK GS ++V GVTAAQ DVEVSFKVK  +KA V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFK------REGKYVVLMCSDQ 491
           PQ +C+Q   +  SG+GPFGL++LAS D++EYTSV+F++FK      ++ KYVVLMCSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494

Query: 492 SRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLAIG 551
           SRSSLN  NDK+T+GAFV +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAIG
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIG 554

Query: 552 ENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
           EN  L+ FN  T+ V I  L+AWSL  A IN
Sbjct: 555 ENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of MC09g0207 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.9e-203
Identity = 344/575 (59.83%), Postives = 429/575 (74.61%), Query Frame = 0

Query: 10  SFLSVFLCHG---VFKLEASHHVYRAQTSASVPPDHQ-----QPYRTSYHFQPPKNWM-- 69
           S   + L HG   + K +A HH +       +  DHQ      PYRT+YHFQ PKNWM  
Sbjct: 12  SISMMLLSHGMIIIAKDQAIHH-HDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMND 71

Query: 70  -NGPMIYKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 129
            NGPMIYKGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P DINGCW
Sbjct: 72  PNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGCW 131

Query: 130 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMA--PTPQN 189
           SGS TILP NKP +LYTGIN +N+QVQNLA+PKNLSDP+L EW+K P+NPLMA  PT  N
Sbjct: 132 SGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNNN 191

Query: 190 NINSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTG 249
           NIN+SSFRDP+TAW   D +WRV++G++   RGLA+++ S DF++W+   NPLH  +  G
Sbjct: 192 NINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGNG 251

Query: 250 MWECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKG 309
           +WECPDFFPV     +G DTS+ G  VKHVLK+SL DT++++YT+G Y+++KD+YVPD+G
Sbjct: 252 IWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPDEG 311

Query: 310 SVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIW 369
           S+E   GLRYDYGK YASK+FFD E  RR+LWGWVNESS   DD KKGWSGVQAIPR++ 
Sbjct: 312 SIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRTVV 371

Query: 370 LDASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKK 429
           LD SGKQLVQWP+ E+  LR++ V+L ++V+K GS++E+  +TA+Q DVE+SFK+     
Sbjct: 372 LDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPESNY 431

Query: 430 AQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLM 489
            + L     +PQ+LC+QKGA+ +   GPFGLL LAS  L+EYT+VFF++FK   KYVVLM
Sbjct: 432 VEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYVVLM 491

Query: 490 CSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPT 549
           CSDQ+RSSLN   DK ++G FVD+DP+ E+LSLR LIDHSIVESF  KGK+CITARVYPT
Sbjct: 492 CSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARVYPT 551

Query: 550 LAIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
           +AI +   LY FNN TE V I+KL+AWS+ KA IN
Sbjct: 552 MAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of MC09g0207 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 690.3 bits (1780), Expect = 1.9e-197
Identity = 336/535 (62.80%), Postives = 410/535 (76.64%), Query Frame = 0

Query: 44  QPYRTSYHFQPPKNWM---NGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTDLINW 103
           QPYRT YHFQPPKNWM   NGPMIYKGIYHLFYQ+N  GAV      VW H+TSTDLINW
Sbjct: 40  QPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINW 99

Query: 104 VPHQHAIFPSQPSDINGCWSGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLI 163
           +    AI PS+PSDINGCWSGS TILP  KP +LYTG +  N QVQNL  PKNL+DP+L 
Sbjct: 100 ITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLR 159

Query: 164 EWVKSPKNPLMAPTPQNNINSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDF 223
            W KSP+NPL+ P+P N+INSS+FRDPTTAW GRD +WR+  GS+   RGLAI++ SKDF
Sbjct: 160 HWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDF 219

Query: 224 IQWDQVNNPLHYADDTGMWECPDFFPVAKNSRVGVDTSV-NGPRVKHVLKVSLDDTKHDH 283
           + W Q   PLHY D TGMWECPDFFPVA+    G+DTS  +GP VKHVLKVSL DT +D+
Sbjct: 220 VIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDY 279

Query: 284 YTVGTYNVKKDVYVPDKGSVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVD 343
           YT+GTY+  +DVYVPDKG V+     RYDYGK YASKTF+DS  +RR+LWGWVNESS   
Sbjct: 280 YTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEK 339

Query: 344 DDKKKGWSGVQAIPRSIWLDASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGV 403
           D+ KKGW+G+QAIPR +WLD SGK+LVQWP+ EI++LR  +VK  NK+LK GSV+EV GV
Sbjct: 340 DNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGV 399

Query: 404 TAAQVDVEVSFKVKNF--KKAQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLK 463
           TA Q DVEV FKV  F  +KA V++P W DPQL+C+Q+ A+  SG+GPFGL++LASK+++
Sbjct: 400 TAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNAS--SGLGPFGLMVLASKNME 459

Query: 464 EYTSVFFKVFK---REGKYVVLMCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLI 523
           EYTSV  ++F+      ++VV+MCSDQS SSL   NDKTTYGAF+D+ P  + +SLR+LI
Sbjct: 460 EYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPISLRTLI 519

Query: 524 DHSIVESFGVKGKACITARVYPTLAIGENVGLYAFNNATEGVTISKLTAWSLNKA 568
           D SIVESFG KGK CIT+RVYP LAIGE   L+AFN  ++ V +  L+AWS+  +
Sbjct: 520 DKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 571

BLAST of MC09g0207 vs. NCBI nr
Match: XP_022153896.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Momordica charantia])

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 570/573 (99.48%), Postives = 570/573 (99.48%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
           VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN
Sbjct: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 573

BLAST of MC09g0207 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 476/573 (83.07%), Postives = 523/573 (91.27%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MASFSL F SFL     HGV +L+ASHHVYRAQ S S+P  +QQPYRTSYHFQPPKNW+N
Sbjct: 1   MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHYQQPYRTSYHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH HAI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN+I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLG D +WRV+IGSKINARGLAIMY+SKDF++WDQV++PLHYAD TGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAK  R GVDTSV+G  VKHVLKVSLDDTKHDHYTVG Y+++KD+YVP+KGS+
Sbjct: 241 ECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNKGSI 300

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGY GLRYDYGK YASKTF+D +KKRRVLWGWVNESSSV+DD KKGWSG+QAIPR+IWLD
Sbjct: 301 EGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTIWLD 360

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQL+QWPI EIQKLRKDKVKL NKVLKKGS+IEVKGVTAAQ DVEVSFKVKNFKKAQ
Sbjct: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
           +LKPEWKDPQLLC+QKGA  + GVG FGLL+LASKDLKEYTS+ F +FKR+GKY+VLMCS
Sbjct: 421 ILKPEWKDPQLLCSQKGATTKGGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLN NNDKTTYGAF+++DPV E+LSLRSLIDHS+VESFG KGKACITARVYPTLA
Sbjct: 481 DQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGKACITARVYPTLA 540

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           IG+NVGLY FNN TE VTI+KL AWSL KA IN
Sbjct: 541 IGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573

BLAST of MC09g0207 vs. NCBI nr
Match: KAG6575469.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 993 bits (2566), Expect = 0.0
Identity = 472/570 (82.81%), Postives = 522/570 (91.58%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MAS SL F SFL   LCHG  KL+ASHHVYRAQ S  +   ++QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYRAQASRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCWSGS 120
           GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH +AI+PSQPSDINGCWSGS
Sbjct: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120

Query: 121 ATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNINSS 180
           ATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN INSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180

Query: 181 SFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMWECP 240
           SFRDPTTAWLG D +WRV+IGSKI+ARGLAIM++SKDF++WDQV++PLHYAD TGMWECP
Sbjct: 181 SFRDPTTAWLGPDSEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMWECP 240

Query: 241 DFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSVEGY 300
           DFFPVAKN R GVDTSV+G  VKHVLKVSLDDTKHDHYTVGTY++KKD+YVPDKGS+EGY
Sbjct: 241 DFFPVAKNGRAGVDTSVSGRHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKGSIEGY 300

Query: 301 AGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLDASG 360
            GLRYDYGK YASKTFFDS+KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLDASG
Sbjct: 301 TGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASG 360

Query: 361 KQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQVLK 420
           KQL+QWPIVEIQKLRKDKVKL NKVLKKGS+I VKGVTAAQ DV+VSFKVK+F KAQ LK
Sbjct: 361 KQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQPLK 420

Query: 421 PEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCSDQS 480
            EWKDPQL+C+Q GAA + GVGPFGLL+LAS +L+EYTSVFF++FKR+ KYVVLMCSDQS
Sbjct: 421 AEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQS 480

Query: 481 RSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLAIGE 540
           RSSLN+NNDKTTYGAF+D+DPVREN+SLRSLIDHSIVESFG KGKACITARVYP LAIG+
Sbjct: 481 RSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLAIGD 540

Query: 541 NVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
             GLYAFNN TE VTI+KL+AWS+ KA I+
Sbjct: 541 KAGLYAFNNGTESVTITKLSAWSMKKAQIS 570

BLAST of MC09g0207 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 469/573 (81.85%), Postives = 520/573 (90.75%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MAS SL F SFL   LCHG  KL+ASHHVY  Q S  +   ++QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH +AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN+I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLG D +WRV+IGSKI+ARGLAIM++SKDF++WDQV++PLHYAD TGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKNSR GVDTSV+G  VKHVLKVSLDDTKHD YTVGTY++KKD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGY GLRYDYGK YASKTFFD +KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQL+QWPIVEIQKLRKDKVKL NKVLKKGS+I VKGVTAAQ DV+VSFKVK+F KAQ
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
            LK EWKDPQL+C+Q GAA + GVGPFGLL+LAS +L+EYTSVFF++FKR+ KYVVLMCS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAF+D+DPVREN+SLRSLIDHSIVESFG KGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           IG+  GLYAFNN TE VTI+KL+AWS+ KA I+
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of MC09g0207 vs. NCBI nr
Match: XP_022992217.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita maxima])

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 467/573 (81.50%), Postives = 512/573 (89.35%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MAS SL F S L   LCHG  KL+ASHHVY AQ S  +   ++QPYRTSYHFQPPKNW+N
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH HAI+PSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPG KPA+LYTGINP+N QVQNLA+PKNLSDP+L EWVKSP NPLMAPTPQN I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLG D QWRV+IGSKI+ARGLAIM+RSKDF++WDQV++PLHYAD TGMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKN R G+DTSVNG  VKHVLKVSLDDTKHDHYTVGTY++KKD+YVPDK S 
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGY GLRYDYGK YASKTFFD +KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQL+QWPI EIQKLRKDK+KL NKV+KKGS+I VKGVTAAQ DVEVSFKVK+FKKAQ
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
            LK EWKDPQL C++ GAA   GVGPFGLL+LASKDL+EYTSVFF+VFK + KYVVLMCS
Sbjct: 423 RLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMCS 482

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAF+D+DPVREN+SLRSLIDHSIVESFG KGKACITARVYP LA
Sbjct: 483 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPKLA 542

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           +G+  GLYAFNN TE VTI+KL+AWS+ KA I+
Sbjct: 543 MGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575

BLAST of MC09g0207 vs. ExPASy TrEMBL
Match: A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 570/573 (99.48%), Postives = 570/573 (99.48%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
           VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS
Sbjct: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA
Sbjct: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN
Sbjct: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 573

BLAST of MC09g0207 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 469/573 (81.85%), Postives = 520/573 (90.75%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MAS SL F SFL   LCHG  KL+ASHHVY  Q S  +   ++QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH +AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN+I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLG D +WRV+IGSKI+ARGLAIM++SKDF++WDQV++PLHYAD TGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKNSR GVDTSV+G  VKHVLKVSLDDTKHD YTVGTY++KKD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGY GLRYDYGK YASKTFFD +KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQL+QWPIVEIQKLRKDKVKL NKVLKKGS+I VKGVTAAQ DV+VSFKVK+F KAQ
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
            LK EWKDPQL+C+Q GAA + GVGPFGLL+LAS +L+EYTSVFF++FKR+ KYVVLMCS
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAF+D+DPVREN+SLRSLIDHSIVESFG KGKACITARVYP LA
Sbjct: 481 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           IG+  GLYAFNN TE VTI+KL+AWS+ KA I+
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of MC09g0207 vs. ExPASy TrEMBL
Match: A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 467/573 (81.50%), Postives = 512/573 (89.35%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MAS SL F S L   LCHG  KL+ASHHVY AQ S  +   ++QPYRTSYHFQPPKNW+N
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH HAI+PSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPG KPA+LYTGINP+N QVQNLA+PKNLSDP+L EWVKSP NPLMAPTPQN I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLG D QWRV+IGSKI+ARGLAIM+RSKDF++WDQV++PLHYAD TGMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKN R G+DTSVNG  VKHVLKVSLDDTKHDHYTVGTY++KKD+YVPDK S 
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           EGY GLRYDYGK YASKTFFD +KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQL+QWPI EIQKLRKDK+KL NKV+KKGS+I VKGVTAAQ DVEVSFKVK+FKKAQ
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
            LK EWKDPQL C++ GAA   GVGPFGLL+LASKDL+EYTSVFF+VFK + KYVVLMCS
Sbjct: 423 RLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMCS 482

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLN+NNDKTTYGAF+D+DPVREN+SLRSLIDHSIVESFG KGKACITARVYP LA
Sbjct: 483 DQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPKLA 542

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           +G+  GLYAFNN TE VTI+KL+AWS+ KA I+
Sbjct: 543 MGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575

BLAST of MC09g0207 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 458/572 (80.07%), Postives = 512/572 (89.51%), Query Frame = 0

Query: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60
           MAS SL F SFL   LCHG  KL+ASHHVY  Q S  +   H+QPYRTS HFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  ---GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120
              GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH +AI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180
           SGSATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN+I
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240
           NSSSFRDPTTAWLG D +WRV+IGSK++ RGLAIM+RSKDF++WDQV++PLHYAD  GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300
           ECPDFFPVAKN R GVDTSV+G  VKHVLKVSLDDTKHD YTVGTY++KKD+YVPDKGS+
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360
           + Y+GLRYDYGK YASKTFFD +KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR++WLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420
           ASGKQL+QWPI EIQKLRKDK+KL NKVL+KGS+I VKGVTAAQ DV+VSFKVK+F KA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 VLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCS 480
           +LK EWKDPQL+C+Q GAA + GVGPFGLL+LAS +L+EYTSVFF++FKR+ KYVVLMCS
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCS 480

Query: 481 DQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLA 540
           DQSRSSLN++NDKT YGAF+D+DPVR+N+SLRSLIDHSIVESFG KGKACITARVYP LA
Sbjct: 481 DQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYPRLA 540

Query: 541 IGENVGLYAFNNATEGVTISKLTAWSLNKAHI 569
           IG+  GLYAFNN TE VTI+KL AWSL KA I
Sbjct: 541 IGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572

BLAST of MC09g0207 vs. ExPASy TrEMBL
Match: A0A2I4JRK2 (Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 462/571 (80.91%), Postives = 508/571 (88.97%), Query Frame = 0

Query: 11  FLSVFLC--HGVFKLEASHHVYRA--QTSASVPPDHQQ-PYRTSYHFQPPKNWMN---GP 70
           FL +FL   H   +L+A HHVYRA  +TS  V   HQQ PYRTSYHFQPPKNW+N   GP
Sbjct: 15  FLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPPKNWINDPNGP 74

Query: 71  MIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCWSGSAT 130
           MIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINW PH HAIFPSQPSDINGCWSGSAT
Sbjct: 75  MIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSAT 134

Query: 131 ILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNINSSSF 190
           ILPG KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTPQN+IN SSF
Sbjct: 135 ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSF 194

Query: 191 RDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMWECPDF 250
           RDPTTAWLGRD +WRV+IGSKI+ARGLAIMYRSKDF++W+QV++PL Y DD GMWECPDF
Sbjct: 195 RDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDF 254

Query: 251 FPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSVEGYAG 310
           FPVAK  R GV+  V+G  VKH+LKVSLDDTKHD YTVGTYN KKD+Y+P+KGS+EGY G
Sbjct: 255 FPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNG 314

Query: 311 LRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLDASGKQ 370
           LRYDYGK YASKTFFDS+KKRRVLWGWVNESSSVDDD KKGWSGVQAIPR+IWLDASGKQ
Sbjct: 315 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQ 374

Query: 371 LVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQVLKPE 430
           L+QWPIVEIQKLR +KVKL N+VLKKGS IEVKGVTAAQ DVE+SFKV+N KKA++LKPE
Sbjct: 375 LIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPE 434

Query: 431 WKDPQLLCAQKGAAK--ESG-VGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMCSDQ 490
           WKDPQLLC+QKG +   E G VGPFGLL+LAS DLKEYTSVFF VFK+  KYVVLMCSDQ
Sbjct: 435 WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKPHKYVVLMCSDQ 494

Query: 491 SRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLAIG 550
           SRSSL+SNNDKTTYGAF+D+DP REN+SLRSLIDHSIVESFG+KGK CITARVYPTLAIG
Sbjct: 495 SRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 554

Query: 551 ENVGLYAFNNATEGVTISKLTAWSLNKAHIN 570
           +NV LYAFNN T+ VTI+KLTAWS+ KAHIN
Sbjct: 555 DNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 585

BLAST of MC09g0207 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 781.6 bits (2017), Expect = 4.4e-226
Identity = 377/578 (65.22%), Postives = 457/578 (79.07%), Query Frame = 0

Query: 2   ASFSLWFFSFLSVFLCHGVFKLEASHHVYR--AQTSASVPPDHQQPYRTSYHFQPPKNWM 61
           ++  LW    L++ + + V  LEASHHVY+   Q++ +  P   QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWL--LLTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCWSG 121
           NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AIFPS P DINGCWSG
Sbjct: 67  NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSG 126

Query: 122 SATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNINS 181
           SATILP  KP +LYTGI+P+N QVQN+A PKNLSDP+L EW KSP NPLMAP   N IN+
Sbjct: 127 SATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINA 186

Query: 182 SSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMWEC 241
           SSFRDPTTAWLG+D++WRV+IGSKI+ RGLAI Y SKDF++W++   PLHY D +GMWEC
Sbjct: 187 SSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWEC 246

Query: 242 PDFFPVAKNSRVGVDTSVNGPR---VKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGS 301
           PDFFPV +    GV+TS  G     +KHVLK+SLDDTKHD+YT+GTY+  KD +VPD G 
Sbjct: 247 PDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGF 306

Query: 302 VEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWL 361
                  RYDYGK YASKTFFDS K RR+LWGW NESSSV+DD +KGWSG+Q IPR IWL
Sbjct: 307 KMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWL 366

Query: 362 DASGKQLVQWPIVEIQKLRKDKVK-LINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKK 421
           D SGKQL+QWP+ E+++LR  +VK L NKVLK GS +EV GVTAAQ DVEV FKV++ +K
Sbjct: 367 DRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEK 426

Query: 422 AQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFK---REGKYV 481
           A V++P W DPQL+C++   + +SG+GPFGL++LASK+L+EYTSV+F++FK      KYV
Sbjct: 427 ADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYV 486

Query: 482 VLMCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARV 541
           VLMCSDQSRSSL  +NDKTTYGAFVD++P  + LSLR+LIDHS+VESFG KG+ACIT+RV
Sbjct: 487 VLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRV 546

Query: 542 YPTLAIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
           YP LAIG++  L+AFN   + V +  L AWS+N A I+
Sbjct: 547 YPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of MC09g0207 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 776.2 bits (2003), Expect = 1.9e-224
Identity = 377/581 (64.89%), Postives = 457/581 (78.66%), Query Frame = 0

Query: 2   ASFSLWFFSFLSVFLCHGVFKLEASHHVYR--AQTSASVPPDHQQPYRTSYHFQPPKNWM 61
           ++  LW    L++ + + V  LEASHHVY+   Q++ +  P   QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWL--LLTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  ---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGC 121
              NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AIFPS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNN 181
           WSGSATILP  KP +LYTGI+P+N QVQN+A PKNLSDP+L EW KSP NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGM 241
           IN+SSFRDPTTAWLG+D++WRV+IGSKI+ RGLAI Y SKDF++W++   PLHY D +GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKNSRVGVDTSVNGPR---VKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPD 301
           WECPDFFPV +    GV+TS  G     +KHVLK+SLDDTKHD+YT+GTY+  KD +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRS 361
            G        RYDYGK YASKTFFDS K RR+LWGW NESSSV+DD +KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDASGKQLVQWPIVEIQKLRKDKVK-LINKVLKKGSVIEVKGVTAAQVDVEVSFKVKN 421
           IWLD SGKQL+QWP+ E+++LR  +VK L NKVLK GS +EV GVTAAQ DVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFK---REG 481
            +KA V++P W DPQL+C++   + +SG+GPFGL++LASK+L+EYTSV+F++FK      
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLMCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACIT 541
           KYVVLMCSDQSRSSL  +NDKTTYGAFVD++P  + LSLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
           +RVYP LAIG++  L+AFN   + V +  L AWS+N A I+
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of MC09g0207 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 711.4 bits (1835), Expect = 5.6e-205
Identity = 350/571 (61.30%), Postives = 418/571 (73.20%), Query Frame = 0

Query: 12  LSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWM---NGPMIYKGI 71
           LS+FL + +  LEAS H            D  QPYRT YHFQP KNWM   NGPMIYKGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCWSGSATILPGN 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNINSSSFRDPTT 191
           KP +LYTGI+    QVQN+AVP N+SDP+L EW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGRDRQWRVVIGSKI-NARGLAIMYRSKDFIQWDQVNNPLHYADDTGMWECPDFFPVA 251
           AWLGRD +WRV++GS   + RGLAI+Y+S+DF  W Q   PLHY D TGMWECPDFFPV+
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KNSRVGVDTSVNGPR-VKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSVEGYAGLRY 311
                GV+TS  G   +KHVLKVSL +T HD+YT+G+Y+ +KDVYVPD G V+  +  R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLDASGKQLVQ 371
           DYGK YASKTF+D  KKRR+LWGWVNESS   DD +KGWSG+Q+ PR IWLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQVLKPEWKD 431
           WPI EI+ LR  +V    KVLK GS ++V GVTAAQ DVEVSFKVK  +KA V++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFK------REGKYVVLMCSDQ 491
           PQ +C+Q   +  SG+GPFGL++LAS D++EYTSV+F++FK      ++ KYVVLMCSDQ
Sbjct: 435 PQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQ 494

Query: 492 SRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTLAIG 551
           SRSSLN  NDK+T+GAFV +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAIG
Sbjct: 495 SRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIG 554

Query: 552 ENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
           EN  L+ FN  T+ V I  L+AWSL  A IN
Sbjct: 555 ENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of MC09g0207 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 695.7 bits (1794), Expect = 3.2e-200
Identity = 336/532 (63.16%), Postives = 410/532 (77.07%), Query Frame = 0

Query: 44  QPYRTSYHFQPPKNWMNGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTDLINWVPH 103
           QPYRT YHFQPPKNWMNGPMIYKGIYHLFYQ+N  GAV      VW H+TSTDLINW+  
Sbjct: 40  QPYRTGYHFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINWITL 99

Query: 104 QHAIFPSQPSDINGCWSGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWV 163
             AI PS+PSDINGCWSGS TILP  KP +LYTG +  N QVQNL  PKNL+DP+L  W 
Sbjct: 100 SPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLRHWT 159

Query: 164 KSPKNPLMAPTPQNNINSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQW 223
           KSP+NPL+ P+P N+INSS+FRDPTTAW GRD +WR+  GS+   RGLAI++ SKDF+ W
Sbjct: 160 KSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFVIW 219

Query: 224 DQVNNPLHYADDTGMWECPDFFPVAKNSRVGVDTSV-NGPRVKHVLKVSLDDTKHDHYTV 283
            Q   PLHY D TGMWECPDFFPVA+    G+DTS  +GP VKHVLKVSL DT +D+YT+
Sbjct: 220 KQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDYYTI 279

Query: 284 GTYNVKKDVYVPDKGSVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDK 343
           GTY+  +DVYVPDKG V+     RYDYGK YASKTF+DS  +RR+LWGWVNESS   D+ 
Sbjct: 280 GTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKDNI 339

Query: 344 KKGWSGVQAIPRSIWLDASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAA 403
           KKGW+G+QAIPR +WLD SGK+LVQWP+ EI++LR  +VK  NK+LK GSV+EV GVTA 
Sbjct: 340 KKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGVTAP 399

Query: 404 QVDVEVSFKVKNF--KKAQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYT 463
           Q DVEV FKV  F  +KA V++P W DPQL+C+Q+ A+  SG+GPFGL++LASK+++EYT
Sbjct: 400 QADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNAS--SGLGPFGLMVLASKNMEEYT 459

Query: 464 SVFFKVFK---REGKYVVLMCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHS 523
           SV  ++F+      ++VV+MCSDQS SSL   NDKTTYGAF+D+ P  + +SLR+LID S
Sbjct: 460 SVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPISLRTLIDKS 519

Query: 524 IVESFGVKGKACITARVYPTLAIGENVGLYAFNNATEGVTISKLTAWSLNKA 568
           IVESFG KGK CIT+RVYP LAIGE   L+AFN  ++ V +  L+AWS+  +
Sbjct: 520 IVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of MC09g0207 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 676.8 bits (1745), Expect = 1.5e-194
Identity = 326/525 (62.10%), Postives = 393/525 (74.86%), Query Frame = 0

Query: 55  PKNWMNGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHQHAIFPSQPSDI 114
           P   ++GPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDI
Sbjct: 35  PNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDI 94

Query: 115 NGCWSGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTP 174
           NGCWSGS TILP  KP +LYTGI+    QVQN+AVP N+SDP+L EW K P+NPLM    
Sbjct: 95  NGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNA 154

Query: 175 QNNINSSSFRDPTTAWLGRDRQWRVVIGSKI-NARGLAIMYRSKDFIQWDQVNNPLHYAD 234
            N IN   FRDPTTAWLGRD +WRV++GS   + RGLAI+Y+S+DF  W Q   PLHY D
Sbjct: 155 VNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 214

Query: 235 DTGMWECPDFFPVAKNSRVGVDTSVNGPR-VKHVLKVSLDDTKHDHYTVGTYNVKKDVYV 294
            TGMWECPDFFPV+     GV+TS  G   +KHVLKVSL +T HD+YT+G+Y+ +KDVYV
Sbjct: 215 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 274

Query: 295 PDKGSVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIP 354
           PD G V+  +  R DYGK YASKTF+D  KKRR+LWGWVNESS   DD +KGWSG+Q+ P
Sbjct: 275 PDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFP 334

Query: 355 RSIWLDASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVK 414
           R IWLD SGK+L+QWPI EI+ LR  +V    KVLK GS ++V GVTAAQ DVEVSFKVK
Sbjct: 335 RKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVK 394

Query: 415 NFKKAQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFK----- 474
             +KA V++P W DPQ +C+Q   +  SG+GPFGL++LAS D++EYTSV+F++FK     
Sbjct: 395 ELEKADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDT 454

Query: 475 -REGKYVVLMCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGK 534
            ++ KYVVLMCSDQSRSSLN  NDK+T+GAFV +DP  + +SLR+LIDHSIVES+G  G+
Sbjct: 455 NKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGR 514

Query: 535 ACITARVYPTLAIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 571
            CIT+RVYP LAIGEN  L+ FN  T+ V I  L+AWSL  A IN
Sbjct: 515 TCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 559