MC09g0091 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0091
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	alpha-amylase/subtilisin inhibitor-like
Location	MC09: 912734 .. 913243 (+)
RNA-Seq Expression	MC09g0091
Synteny	MC09g0091

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTCAGCCGACGAGGTTCCGGTGCTCGACTCCGATGGACATCGCATTGTATCGGGTGCACAATACTACATAAAGCCTGCACTTTCTGGTAGAGGAGGAGCTCTCACTTTGGTCACCAGAAAAAATGGCAAGCCATGCCCGAAATACGTCGGTCAGGTAGAGCATCCTGATCAGAAAGGCACCCCAGTAAGATTTACGTTGTCTCCAGTGACCAACGAAGGTTGGCGCGTACATATTACGTTTCAAGCGTCGAGTTCCTGCTCAAAATCAGCCATGTGGGGGCTCGGCGATCGGGAGAATGTGACAGGAAGACGGCTGATCACGGCAGGGTCGGAACTTTTGGTTGAAGTCTTGTTCAAGATTGTGAAAAACAATGGACTTTACCATCTTCTTTGGTGTACTTCAGAAGATCACAATCCTCAGTGTGGAACTGCTGGTATTGTCGGTGAGGATGGAGTGAGTTTGTTGGCTTTGGATGGCCCCGAGCTTCCTTTTGTCTTTGAGAAAGTT

mRNA sequence

GCCTCAGCCGACGAGGTTCCGGTGCTCGACTCCGATGGACATCGCATTGTATCGGGTGCACAATACTACATAAAGCCTGCACTTTCTGGTAGAGGAGGAGCTCTCACTTTGGTCACCAGAAAAAATGGCAAGCCATGCCCGAAATACGTCGGTCAGGTAGAGCATCCTGATCAGAAAGGCACCCCAGTAAGATTTACGTTGTCTCCAGTGACCAACGAAGGTTGGCGCGTACATATTACGTTTCAAGCGTCGAGTTCCTGCTCAAAATCAGCCATGTGGGGGCTCGGCGATCGGGAGAATGTGACAGGAAGACGGCTGATCACGGCAGGGTCGGAACTTTTGGTTGAAGTCTTGTTCAAGATTGTGAAAAACAATGGACTTTACCATCTTCTTTGGTGTACTTCAGAAGATCACAATCCTCAGTGTGGAACTGCTGGTATTGTCGGTGAGGATGGAGTGAGTTTGTTGGCTTTGGATGGCCCCGAGCTTCCTTTTGTCTTTGAGAAAGTT

Coding sequence (CDS)

GCCTCAGCCGACGAGGTTCCGGTGCTCGACTCCGATGGACATCGCATTGTATCGGGTGCACAATACTACATAAAGCCTGCACTTTCTGGTAGAGGAGGAGCTCTCACTTTGGTCACCAGAAAAAATGGCAAGCCATGCCCGAAATACGTCGGTCAGGTAGAGCATCCTGATCAGAAAGGCACCCCAGTAAGATTTACGTTGTCTCCAGTGACCAACGAAGGTTGGCGCGTACATATTACGTTTCAAGCGTCGAGTTCCTGCTCAAAATCAGCCATGTGGGGGCTCGGCGATCGGGAGAATGTGACAGGAAGACGGCTGATCACGGCAGGGTCGGAACTTTTGGTTGAAGTCTTGTTCAAGATTGTGAAAAACAATGGACTTTACCATCTTCTTTGGTGTACTTCAGAAGATCACAATCCTCAGTGTGGAACTGCTGGTATTGTCGGTGAGGATGGAGTGAGTTTGTTGGCTTTGGATGGCCCCGAGCTTCCTTTTGTCTTTGAGAAAGTT

Protein sequence

ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFTLSPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLVEVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEKV

Homology

[Close]

BLAST of MC09g0091 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.7e-24
Identity = 67/176 (38.07%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 8   VLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFT- 67
           V+D+ G  +    +Y+I+PA++G GG  TLVT  NG PCP +VG        G  V+FT 
Sbjct: 27  VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT-GNG-PCPLHVGLDNTEGTLGVAVKFTP 86

Query: 68  LSPVTNE-----GWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLVEV---L 127
            +P  ++        + +TF  S+SC +S  W LG+++  +GRRLI  G +         
Sbjct: 87  FAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDATSGRRLIVTGRDNGAGSQGNF 146

Query: 128 FKIV--KNNGLYHLLWCTSE---DHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 170
           F+IV  +  G Y++ WC +E       QCGT G++ E+G +LLALDG  LP VF+K
Sbjct: 147 FRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of MC09g0091 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.1e-23
Identity = 69/179 (38.55%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 8   VLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFTL 67
           V+D+ G  + +   Y+I+PA++G GG+LTLVTR +   CP  VG ++    +G  V   L
Sbjct: 27  VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRNS---CPFNVG-LDPDAPQGFAV--LL 86

Query: 68  SP-VTNE-------GWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV---- 127
           SP V+N        G  + + FQA +SC +S  W LG+R+  TGRR I  G +       
Sbjct: 87  SPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRDDSTVGSY 146

Query: 128 EVLFKIVK--NNGLYHLLWCTSE---DHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 170
              F+IV+  + G++++ WC +E       +CGT GIV E+G  LLALDG  LP  F+K
Sbjct: 147 GNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of MC09g0091 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.7e-22
Identity = 66/177 (37.29%), Postives = 102/177 (57.63%), Query Frame = 0


Query: 8   VLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFT- 67
           VLD+ G  +    +Y+I+P ++ +GG  T+V+R   + CP +VG       +G  V+FT 
Sbjct: 29  VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR--NESCPLHVGLELTGLGRGLVVKFTP 88

Query: 68  LSPVTN-EGWRVH----ITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLVE----V 127
            +P  + +  RV+    ITFQASSSC +S  W LG+++  +GRRLI  G++         
Sbjct: 89  FAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEKDTKSGRRLIITGTDSATNGSYGN 148

Query: 128 LFKIVKN--NGLYHLLWCTSE---DHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 170
            F+IV+    G+Y++ WC +E       +CGT  ++ E+G  LLALDG  LP VF+K
Sbjct: 149 FFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNENGKILLALDGGPLPLVFQK 203

[Close]

BLAST of MC09g0091 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.7e-14
Identity = 59/181 (32.60%), Postives = 80/181 (44.20%), Query Frame = 0


Query: 2   SADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGT 61
           SA   PV D++GH + +   YY+ PA  G GG LT+  R    PCP  V Q     +KG 
Sbjct: 21  SAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV--LPCPLLVAQETDERRKGF 80

Query: 62  PVRFT-----LSP---VTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAG--- 121
           PVRFT      +P          V I F A++ C +S  W +GD E +TG R +  G   
Sbjct: 81  PVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGD-EPLTGARRVVTGPLI 140

Query: 122 --SELLVEVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFE 170
             S    E  F++ K  G Y L+ C        C   G+  +   + L    P    VF+
Sbjct: 141 GPSPSGRENAFRVEKYGGGYKLVSC-----RDSCQDLGVSRDGARAWLGASQPPHVVVFK 193

[Close]

BLAST of MC09g0091 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-12
Identity = 54/184 (29.35%), Postives = 84/184 (45.65%), Query Frame = 0


Query: 8   VLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFTL 67
           V+D DG+ +     YY+ P + GRGG LTL  R  G+PCP  + Q      +G PV+F+ 
Sbjct: 30  VVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGR-GGQPCPYDIVQESSEVDEGIPVKFS- 89

Query: 68  SPVTNEGWRVHITFQASSS------------CSKSAMWGLGDRENVTGRRLITAG----- 127
                  WR+ + F   S             C +S  W +G+ ++   +  + AG     
Sbjct: 90  ------NWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEG 149

Query: 128 -SELLVEVLFKIVKN-NGLYHLLWC--TSEDHNPQCGTAGI-VGEDGVSLLALDGPELPF 170
             +  ++  FKI K+    Y  ++C  T +  NP+C   GI + E GV  LAL       
Sbjct: 150 FGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLV 204

[Close]

BLAST of MC09g0091 vs. NCBI nr
Match: XP_022150757.1 (alpha-amylase/subtilisin inhibitor-like [Momordica charantia])

HSP 1 Score: 309 bits (792), Expect = 2.30e-105
Identity = 144/170 (84.71%), Postives = 158/170 (92.94%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKG 60
           ++A +VPVLD++G RIVSGAQYYIKPA+ GRGGALTLV RKNGKPCPKYVGQVEHPDQKG
Sbjct: 17  SAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQVEHPDQKG 76

Query: 61  TPVRFTLSPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLVEVLFK 120
           TPV+F L+PVTNEGW  HITFQ S SCSK  MWGLGDRENV+GRRLIT+GSELLVEVLFK
Sbjct: 77  TPVKFMLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDRENVSGRRLITSGSELLVEVLFK 136

Query: 121 IVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEKV 170
           IVK+NGLYH+LWCTSEDHNPQCG+AGI+ EDGVSLLALDGPELPFVFEKV
Sbjct: 137 IVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLLALDGPELPFVFEKV 186

[Close]

BLAST of MC09g0091 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 141 bits (356), Expect = 5.12e-39
Identity = 75/174 (43.10%), Postives = 103/174 (59.20%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD+DG  +  G +YYIKPA++   G LTL +R N  PCP YVGQ        
Sbjct: 20  STAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQEPVASTNI 79

Query: 61  GTPVRFTL----SPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV FT       +  E    ++ FQASS+C  S  W + + E+ TGRR +  G++   
Sbjct: 80  GLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGNDNSP 139

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F+I +NNG+Y+++WC      P+CG+AGI+ EDGV LLALDG   PF F K
Sbjct: 140 TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 192

[Close]

BLAST of MC09g0091 vs. NCBI nr
Match: KAG7030262.1 (hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 140 bits (353), Expect = 9.38e-39
Identity = 75/174 (43.10%), Postives = 103/174 (59.20%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD+DG  +  G +YYIKPA++   G LTL +R N  PCP YVGQ        
Sbjct: 5   STAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQEPIASTNI 64

Query: 61  GTPVRFTL----SPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV FT       +  E    ++ FQASS+C  S  W + + E+ TGRR +  G++   
Sbjct: 65  GLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGNDNGP 124

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F+I +NNG+Y+++WC      P+CG+AGI+ EDGV LLALDG   PF F K
Sbjct: 125 TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 177

[Close]

BLAST of MC09g0091 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 140 bits (353), Expect = 1.45e-38
Identity = 75/174 (43.10%), Postives = 103/174 (59.20%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD+DG  +  G +YYIKPA++   G LTL +R N  PCP YVGQ        
Sbjct: 20  STAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQEPIASTNI 79

Query: 61  GTPVRFTL----SPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV FT       +  E    ++ FQASS+C  S  W + + E+ TGRR +  G++   
Sbjct: 80  GLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGNDNGP 139

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F+I +NNG+Y+++WC      P+CG+AGI+ EDGV LLALDG   PF F K
Sbjct: 140 TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 192

[Close]

BLAST of MC09g0091 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 140 bits (353), Expect = 1.45e-38
Identity = 75/174 (43.10%), Postives = 102/174 (58.62%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD+DG  +  G +YYIKPA++   G LTL  R N  PCP YVGQ        
Sbjct: 20  STAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSNA-PCPLYVGQEPVASTNI 79

Query: 61  GTPVRFTL----SPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV FT       +  E    ++ FQASS+C  S  W + + E+ TGRR +  G++   
Sbjct: 80  GLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGNDNGP 139

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F+I +NNG+Y+++WC      P+CG+AGI+ EDGV LLALDG   PF F K
Sbjct: 140 TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 192

[Close]

BLAST of MC09g0091 vs. ExPASy TrEMBL
Match: A0A6J1DBL5 (alpha-amylase/subtilisin inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111018817 PE=3 SV=1)

HSP 1 Score: 309 bits (792), Expect = 1.12e-105
Identity = 144/170 (84.71%), Postives = 158/170 (92.94%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKG 60
           ++A +VPVLD++G RIVSGAQYYIKPA+ GRGGALTLV RKNGKPCPKYVGQVEHPDQKG
Sbjct: 17  SAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQVEHPDQKG 76

Query: 61  TPVRFTLSPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLVEVLFK 120
           TPV+F L+PVTNEGW  HITFQ S SCSK  MWGLGDRENV+GRRLIT+GSELLVEVLFK
Sbjct: 77  TPVKFMLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDRENVSGRRLITSGSELLVEVLFK 136

Query: 121 IVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEKV 170
           IVK+NGLYH+LWCTSEDHNPQCG+AGI+ EDGVSLLALDGPELPFVFEKV
Sbjct: 137 IVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLLALDGPELPFVFEKV 186

[Close]

BLAST of MC09g0091 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 7.03e-39
Identity = 75/174 (43.10%), Postives = 103/174 (59.20%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD+DG  +  G +YYIKPA++   G LTL +R N  PCP YVGQ        
Sbjct: 20  STAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQEPIASTNI 79

Query: 61  GTPVRFTL----SPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV FT       +  E    ++ FQASS+C  S  W + + E+ TGRR +  G++   
Sbjct: 80  GLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGNDNGP 139

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F+I +NNG+Y+++WC      P+CG+AGI+ EDGV LLALDG   PF F K
Sbjct: 140 TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 192

[Close]

BLAST of MC09g0091 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 7.03e-39
Identity = 75/174 (43.10%), Postives = 102/174 (58.62%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD+DG  +  G +YYIKPA++   G LTL  R N  PCP YVGQ        
Sbjct: 20  STAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSNA-PCPLYVGQEPVASTNI 79

Query: 61  GTPVRFTL----SPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV FT       +  E    ++ FQASS+C  S  W + + E+ TGRR +  G++   
Sbjct: 80  GLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGNDNGP 139

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F+I +NNG+Y+++WC      P+CG+AGI+ EDGV LLALDG   PF F K
Sbjct: 140 TGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 192

[Close]

BLAST of MC09g0091 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 128 bits (321), Expect = 4.69e-34
Identity = 70/174 (40.23%), Postives = 102/174 (58.62%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD++G  +  G +YYI PA++  GG LTL +R N  PCP +VGQ        
Sbjct: 20  STAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNA-PCPLFVGQEPVTSTNI 79

Query: 61  GTPVRFTLSP----VTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV F  +     + +EG  ++I F+A S+C+ S  W +   E+ TGRR +  G E   
Sbjct: 80  GLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDTGRRFVGIGDEDGP 139

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F I ++NG Y+++WC +    P+CG AGI+ E+GV L+ALDG   PF F K
Sbjct: 140 AGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIK 192

[Close]

BLAST of MC09g0091 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 128 bits (321), Expect = 4.96e-34
Identity = 70/174 (40.23%), Postives = 102/174 (58.62%), Query Frame = 0


Query: 1   ASADEVPVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQK- 60
           ++A   PVLD++G  +  G +YYI PA++  GG LTL +R N  PCP +VGQ        
Sbjct: 22  STAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSNA-PCPLFVGQEPVTSTNI 81

Query: 61  GTPVRFTLSP----VTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLITAGSELLV 120
           G PV F  +     + +EG  ++I F+A S+C+ S  W +   E+ TGRR +  G E   
Sbjct: 82  GLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDTGRRFVGIGDEDGP 141

Query: 121 EVLFKIVKNNGLYHLLWCTSEDHNPQCGTAGIVGEDGVSLLALDGPELPFVFEK 169
             +F I ++NG Y+++WC +    P+CG AGI+ E+GV L+ALDG   PF F K
Sbjct: 142 AGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIK 194

[Close]

BLAST of MC09g0091 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-13
Identity = 54/184 (29.35%), Postives = 84/184 (45.65%), Query Frame = 0


Query: 8   VLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFTL 67
           V+D DG+ +     YY+ P + GRGG LTL  R  G+PCP  + Q      +G PV+F+ 
Sbjct: 30  VVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGR-GGQPCPYDIVQESSEVDEGIPVKFS- 89

Query: 68  SPVTNEGWRVHITFQASSS------------CSKSAMWGLGDRENVTGRRLITAG----- 127
                  WR+ + F   S             C +S  W +G+ ++   +  + AG     
Sbjct: 90  ------NWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEG 149

Query: 128 -SELLVEVLFKIVKN-NGLYHLLWC--TSEDHNPQCGTAGI-VGEDGVSLLALDGPELPF 170
             +  ++  FKI K+    Y  ++C  T +  NP+C   GI + E GV  LAL       
Sbjct: 150 FGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLV 204

[Close]

BLAST of MC09g0091 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 65.9 bits (159), Expect = 3.7e-11
Identity = 50/171 (29.24%), Postives = 82/171 (47.95%), Query Frame = 0


Query: 7   PVLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKPCPKYVGQVEHPDQKGTPVRFT 66
           PV D +G  +++G  YYI P + GRGG LT+   K  + CP  V Q +    +G PV+F 
Sbjct: 28  PVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT-ETCPTSVIQDQFEVSQGLPVKF- 87

Query: 67  LSPVTNEGWRVHITFQASSSCSKSAMWGLGDRENVTGRRLI-TAGSE-----LLVEVLFK 126
            SP  ++   + ++   +   S +++W L + +  T +  I T G E       V+  FK
Sbjct: 88  -SPY-DKSRTIPVSTDVNIKFSPTSIWELANFDETTKQWFISTCGVEGNPGQKTVDNWFK 147

Query: 127 IVKNNGLYHLLWCTSEDH--NPQCGTAGIVGEDGVSLLALDGPELPFVFEK 170
           I K    Y + +C +  +     C   G+  +DG   LAL    L  +F++
Sbjct: 148 IDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRLALSDVPLKVMFKR 194

[Close]

BLAST of MC09g0091 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 42.4 bits (98), Expect = 4.4e-04
Identity = 39/146 (26.71%), Postives = 58/146 (39.73%), Query Frame = 0


Query: 1   ASADEVP---VLDSDGHRIVSGAQYYIKPALSGRGGALTLVTRKNGKP--CPKY-VGQVE 60
           ++AD  P   VLD  GH + S  QYYI PA  G GG L    R       C    + Q  
Sbjct: 22  SAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSS 81

Query: 61  HPDQKGTPVRFTLSPVTNEGWRVHITFQASSS-------CSKSAMWGLGDRENVTGRRLI 120
            P   G PV  T SP+  +   V ++   +         C  S +W +     +    + 
Sbjct: 82  SPFVSGLPV--TFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHSVQLRKSFVS 141

Query: 121 TAGSELLVEVLFKIVKNNGLYHLLWC 134
             G +      F+I ++   Y L++C
Sbjct: 142 IGGQKGKGNSWFQIQEDGDAYKLMYC 165

The following BLAST results are available for this feature:

BLAST of MC09g0091 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A072TH68	1.7e-24	38.07	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV7	9.1e-23	38.55	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	2.7e-22	37.29	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P29421	2.7e-14	32.60	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]
Q8RXD5	1.5e-12	29.35	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]

BLAST of MC09g0091 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150757.1	2.30e-105	84.71	alpha-amylase/subtilisin inhibitor-like [Momordica charantia]	[more]
XP_023545477.1	5.12e-39	43.10	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
KAG7030262.1	9.38e-39	43.10	hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022946294.1	1.45e-38	43.10	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_022999136.1	1.45e-38	43.10	miraculin-like [Cucurbita maxima]	[more]

BLAST of MC09g0091 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBL5	1.12e-105	84.71	alpha-amylase/subtilisin inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1G3G3	7.03e-39	43.10	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	7.03e-39	43.10	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A5A7VDV8	4.69e-34	40.23	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A5D3D468	4.96e-34	40.23	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]

BLAST of MC09g0091 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	1.0e-13	29.35	kunitz trypsin inhibitor 1	[more]
AT1G17860.1	3.7e-11	29.24	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73325.1	4.4e-04	26.71	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 7..36 score: 36.6 coord: 46..66 score: 25.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 7..170 e-value: 1.5E-24 score: 97.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 8..169 e-value: 4.4E-31 score: 108.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..169
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 8..24
None	No IPR available	GENE3D	2.80.10.50		coord: 2..170 e-value: 4.4E-37 score: 129.4
None	No IPR available	PANTHER	PTHR33107:SF40	TRYPSIN INHIBITOR A	coord: 4..169
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 7..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0091.1	MC09g0091.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity