MC09g0070 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0070
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ribonuclease T(2)
Location	MC09: 687851 .. 688261 (+)
RNA-Seq Expression	MC09g0070
Synteny	MC09g0070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCATTTCATTGCAAGACCGAATGGGCGAATACTGGCAGGACCTAGTCAGCGGAGACAACACCGCGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTCGGCCACGGTGTTTGACGAGTTCAAGTACTTCTGCATGGGTTTGGATACGTATGGCCGCCATGCTATCCTATCATTTCTGGTGCACGGCGGCCTGGTCCCGAGCTCTGCTCAACTGCACCACAAGACTAGCTTTATCAAAGCCATCGCGGACGTGACCTCGAAAAAGGGCGGCGTCATCTGTGCGGCCGACCAGCGGGGCCGAACACAGCTGAAGATGGTGGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCCTGATAATGTCAGTAGTTCCTGCCCTGATAAGTTCTTGTGGCTT

mRNA sequence

ATCATTTCATTGCAAGACCGAATGGGCGAATACTGGCAGGACCTAGTCAGCGGAGACAACACCGCGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTCGGCCACGGTGTTTGACGAGTTCAAGTACTTCTGCATGGGTTTGGATACGTATGGCCGCCATGCTATCCTATCATTTCTGGTGCACGGCGGCCTGGTCCCGAGCTCTGCTCAACTGCACCACAAGACTAGCTTTATCAAAGCCATCGCGGACGTGACCTCGAAAAAGGGCGGCGTCATCTGTGCGGCCGACCAGCGGGGCCGAACACAGCTGAAGATGGTGGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCCTGATAATGTCAGTAGTTCCTGCCCTGATAAGTTCTTGTGGCTT

Coding sequence (CDS)

ATCATTTCATTGCAAGACCGAATGGGCGAATACTGGCAGGACCTAGTCAGCGGAGACAACACCGCGGCTTGGCAGACCAAATGGGCAATCCACGGGACTTGCTCGGCCACGGTGTTTGACGAGTTCAAGTACTTCTGCATGGGTTTGGATACGTATGGCCGCCATGCTATCCTATCATTTCTGGTGCACGGCGGCCTGGTCCCGAGCTCTGCTCAACTGCACCACAAGACTAGCTTTATCAAAGCCATCGCGGACGTGACCTCGAAAAAGGGCGGCGTCATCTGTGCGGCCGACCAGCGGGGCCGAACACAGCTGAAGATGGTGGTTTTGTGTTATAGTAAAGATGGTGCAACAATGATCGACTGCCCTGATAATGTCAGTAGTTCCTGCCCTGATAAGTTCTTGTGGCTT

Protein sequence

IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVSSSCPDKFLWL

Homology

[Close]

BLAST of MC09g0070 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.9e-11
Identity = 38/127 (29.92%), Postives = 65/127 (51.18%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSF 60
           I  LQ ++   W +++  +N   W  +W  HGTCS + F++  YF + +D    + I+  
Sbjct: 59  ISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDII-- 118

Query: 61  LVHGGLVPSSAQLHHKTSFIKAIADVTSKK----GGVICAADQRGR-TQLKMVVLCYSKD 120
              G L P +A  + +T   +AI      K     G+ C  D + + + L  VV C+++D
Sbjct: 119 ---GALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQD 178

Query: 121 GATMIDC 123
           G+T+IDC
Sbjct: 179 GSTLIDC 180

[Close]

BLAST of MC09g0070 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 65.9 bits (159), Expect = 4.2e-10
Identity = 40/132 (30.30%), Postives = 61/132 (46.21%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSAT--VFDEFKYFCMGLDTYGRHAIL 60
           I  L+D++   W  L +G NT  W  +++ HGTCS T  + D   YF  G+  Y    I 
Sbjct: 88  IQDLEDQLNFDWPSL-TGPNTDFWTHEFSKHGTCSITGPITDIHDYFATGIKLYTEFNIT 147

Query: 61  SFLVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGAT 120
           + L    + PS +  +       AI      K G+ C++      QL  V +C  K+  +
Sbjct: 148 AALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCSSG-----QLSTVAVCIDKNSLS 207

Query: 121 MIDCPDNVSSSC 131
           ++DCPD    SC
Sbjct: 208 IMDCPDLQGWSC 213

[Close]

BLAST of MC09g0070 vs. NCBI nr
Match: XP_022143591.1 (ribonuclease 1-like [Momordica charantia])

HSP 1 Score: 276 bits (706), Expect = 1.26e-93
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 8   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 67
           MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 68  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 127
           PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 128 SSCPDKFLWL 137
           SSCPDKFLWL
Sbjct: 121 SSCPDKFLWL 130

[Close]

BLAST of MC09g0070 vs. NCBI nr
Match: XP_038899255.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 238 bits (608), Expect = 2.01e-77
Identity = 109/137 (79.56%), Postives = 123/137 (89.78%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSF 60
           I+SLQDRM +YWQDL +G+   AW +KWAIHGTCSATVFDEFKYFC+GLDTYGRHA+LSF
Sbjct: 81  IVSLQDRMSQYWQDLENGNPIEAWASKWAIHGTCSATVFDEFKYFCLGLDTYGRHAMLSF 140

Query: 61  LVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMI 120
           LVHGGLVPSS++   KTSF+KAIAD+TSKKGGVICA DQRGRTQL+  VLCY+KDG T+I
Sbjct: 141 LVHGGLVPSSSKYLDKTSFVKAIADMTSKKGGVICAVDQRGRTQLQKAVLCYAKDGDTLI 200

Query: 121 DCPDNVSSSCPDKFLWL 137
           DCPDNVSSSCPD FLWL
Sbjct: 201 DCPDNVSSSCPDNFLWL 217

[Close]

BLAST of MC09g0070 vs. NCBI nr
Match: KGN45018.2 (hypothetical protein Csa_016393 [Cucumis sativus])

HSP 1 Score: 199 bits (505), Expect = 8.84e-62
Identity = 93/137 (67.88%), Postives = 108/137 (78.83%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSF 60
           IISLQDRM +YW+DL +G++  AW +KW +HGTCSA  FD+FKYFC+GLDTYGRHAI SF
Sbjct: 80  IISLQDRMNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSF 139

Query: 61  LVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMI 120
           L   GL PSS++   K SFI AIA+ T KKGGVICA DQ  R QL+  VLCY KDG T+I
Sbjct: 140 LDREGLAPSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLI 199

Query: 121 DCPDNVSSSCPDKFLWL 137
           DCPDNVSSSCPD F+WL
Sbjct: 200 DCPDNVSSSCPDNFVWL 216

[Close]

BLAST of MC09g0070 vs. NCBI nr
Match: XP_004149779.2 (ribonuclease MC [Cucumis sativus])

HSP 1 Score: 199 bits (505), Expect = 9.12e-62
Identity = 93/137 (67.88%), Postives = 108/137 (78.83%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSF 60
           IISLQDRM +YW+DL +G++  AW +KW +HGTCSA  FD+FKYFC+GLDTYGRHAI SF
Sbjct: 81  IISLQDRMNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSF 140

Query: 61  LVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMI 120
           L   GL PSS++   K SFI AIA+ T KKGGVICA DQ  R QL+  VLCY KDG T+I
Sbjct: 141 LDREGLAPSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLI 200

Query: 121 DCPDNVSSSCPDKFLWL 137
           DCPDNVSSSCPD F+WL
Sbjct: 201 DCPDNVSSSCPDNFVWL 217

[Close]

BLAST of MC09g0070 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 96.3 bits (238), Expect = 1.51e-21
Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSF 60
           +ISL+ ++   W DL  G+N   W  +W  HG CS   F  F+YF + L+   +  +L+ 
Sbjct: 80  VISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSLFQYFQISLNLLRKFDLLTI 139

Query: 61  LVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQR-GRTQLKMVVLCYSKDGATM 120
           L   GL P ++Q     + +  I   T K  G+ C  + + G++QL  +VLC  KDGAT+
Sbjct: 140 LKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKTGKSQLNEIVLCLEKDGATL 199

Query: 121 IDCPDNVSSSCPDKFLW 136
           IDCP  VS++C   F+W
Sbjct: 200 IDCPTFVSNTCAKSFVW 216

[Close]

BLAST of MC09g0070 vs. ExPASy TrEMBL
Match: A0A6J1CQQ9 (ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1)

HSP 1 Score: 276 bits (706), Expect = 6.08e-94
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 8   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 67
           MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV
Sbjct: 1   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 60

Query: 68  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 127
           PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS
Sbjct: 61  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 120

Query: 128 SSCPDKFLWL 137
           SSCPDKFLWL
Sbjct: 121 SSCPDKFLWL 130

[Close]

BLAST of MC09g0070 vs. ExPASy TrEMBL
Match: A0A0A0K4L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1)

HSP 1 Score: 186 bits (473), Expect = 2.01e-58
Identity = 86/130 (66.15%), Postives = 101/130 (77.69%), Query Frame = 0


Query: 8   MGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSFLVHGGLV 67
           M +YW+DL +G++  AW +KW +HGTCSA  FD+FKYFC+GLDTYGRHAI SFL   GL 
Sbjct: 1   MNQYWKDLENGNSIEAWASKWEMHGTCSAAGFDQFKYFCLGLDTYGRHAIFSFLDREGLA 60

Query: 68  PSSAQLHHKTSFIKAIADVTSKKGGVICAADQRGRTQLKMVVLCYSKDGATMIDCPDNVS 127
           PSS++   K SFI AIA+ T KKGGVICA DQ  R QL+  VLCY KDG T+IDCPDNVS
Sbjct: 61  PSSSKYVAKASFITAIANSTLKKGGVICAVDQYRRIQLQKAVLCYVKDGHTLIDCPDNVS 120

Query: 128 SSCPDKFLWL 137
           SSCPD F+WL
Sbjct: 121 SSCPDNFVWL 130

[Close]

BLAST of MC09g0070 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.31e-22
Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHAILSF 60
           +ISL+ ++   W DL  G+N   W  +W  HG CS   F  F+YF + L+   +  +L+ 
Sbjct: 80  VISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPFSLFQYFQISLNLLRKFDLLTI 139

Query: 61  LVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAADQR-GRTQLKMVVLCYSKDGATM 120
           L   GL P ++Q     + +  I   T K  G+ C  + + G++QL  +VLC  KDGAT+
Sbjct: 140 LKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVKTGKSQLNEIVLCLEKDGATL 199

Query: 121 IDCPDNVSSSCPDKFLW 136
           IDCP  VS++C   F+W
Sbjct: 200 IDCPTFVSNTCAKSFVW 216

[Close]

BLAST of MC09g0070 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.57e-21
Identity = 48/140 (34.29%), Postives = 75/140 (53.57%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYFCMGLDTYGRHA--IL 60
           I +L+ ++  YW D++ G N   WQ +W  HG CS   F++ +YF + L+    H+  +L
Sbjct: 82  IAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPFNQLQYFQITLNIRTNHSYDLL 141

Query: 61  SFLVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICA--ADQRGRTQLKMVVLCYSKDG 120
           + L   GL PS   +        AI   T KK G+ C   A  + + QL  ++LC+ KDG
Sbjct: 142 TILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQLLEIILCFDKDG 201

Query: 121 ATMIDCPDNVSSSCPDKFLW 136
            T+IDC +    +CP +F+W
Sbjct: 202 ITLIDCTEFAGITCPPQFVW 221

[Close]

BLAST of MC09g0070 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.69e-20
Identity = 47/140 (33.57%), Postives = 77/140 (55.00%), Query Frame = 0


Query: 1   IISLQDRMGEYWQDLVSGDNTAAWQTKWAIHGTCSATVFDEFKYF--CMGLDTYGRHAIL 60
           I +L  ++  YW D++SG+N   W+ +W  HGTCS   F++ +YF   + + T   + +L
Sbjct: 78  IATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNYDLL 137

Query: 61  SFLVHGGLVPSSAQLHHKTSFIKAIADVTSKKGGVICAAD-QRGRTQLKMVVLCYSKDGA 120
           + L   GL P++       +   AI   T KK G+ C  + Q  + QL  ++LC+ K+G 
Sbjct: 138 AILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKKKQLFEIILCFDKNGV 197

Query: 121 TMIDCPDNVSSSCPDKFLWL 137
           T+IDC      +CP +F+WL
Sbjct: 198 TLIDCTQFAGITCPSQFVWL 217

The following BLAST results are available for this feature:

BLAST of MC09g0070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	4.9e-11	29.92	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
Q7M438	4.2e-10	30.30	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]

BLAST of MC09g0070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143591.1	1.26e-93	100.00	ribonuclease 1-like [Momordica charantia]	[more]
XP_038899255.1	2.01e-77	79.56	ribonuclease MC-like [Benincasa hispida]	[more]
KGN45018.2	8.84e-62	67.88	hypothetical protein Csa_016393 [Cucumis sativus]	[more]
XP_004149779.2	9.12e-62	67.88	ribonuclease MC [Cucumis sativus]	[more]
XP_031738690.1	1.51e-21	36.50	ribonuclease MC-like [Cucumis sativus]	[more]

BLAST of MC09g0070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQQ9	6.08e-94	100.00	ribonuclease 1-like OS=Momordica charantia OX=3673 GN=LOC111013449 PE=3 SV=1	[more]
A0A0A0K4L1	2.01e-58	66.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G351900 PE=3 SV=1	[more]
A0A0A0LAN6	7.31e-22	36.50	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A6J1F837	1.57e-21	34.29	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]
A0A6J1EXD5	1.69e-20	33.57	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]

BLAST of MC09g0070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 1..136 e-value: 4.3E-27 score: 97.5
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 3..136
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 3..122 e-value: 1.6E-18 score: 67.5
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 5..131
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 5..131
None	No IPR available	CDD	cd00374	RNase_T2	coord: 1..133 e-value: 2.30829E-27 score: 98.2971

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g0070.1	MC09g0070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding