MC08g_new0624 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0624
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	chitinase-3-like protein 2
Location	MC08: 32799741 .. 32802307 (-)
RNA-Seq Expression	MC08g_new0624
Synteny	MC08g_new0624

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCCAAGGCTTGTGCGCGGCGGTTACTGGTTCACCAGCACGGAGGCCGAGCTTCCTTCTGCTAATATTGATGCTACGTTTTTTAGCCATTTGTTTTGTGGATATGTTATTGTTGACCCTGAAACTTACAAGGTCACCATAGATTCCTCGGAGGTTATCCTAATCAATCAATTTAGTTCGACTGTCAGACTGAGCAATCCGAGAGTCAAAACTTTGCTATCCATCAGAGGAGATGGAGATGTACTCACTAAAATGGTTAGCGAGCGTGCAAATCGCGAAGCCTTTATTAATTCCTCGATTGAGGCAGCAATAGAGGGAAAGTTTAACGGCTTAGATCTCGAGTGGCTGTATCCATCTTCTCAAGATGAAATGGAAGGCTTTGAAAACGTTCTCATTGAGTGGCATAGCGCTATTGTTGAGGATGCTAAGAATTACCATAGACAACAGTTGATCCTTGTTGCTGCAGTCTCCAACTTGCCCATCGTGCGCCACAATATTCAGTATCCAATTGATACAATAATACAAACCTTGGATTGGGTTAACCTTATTTCTTATGATTTTTACACCCCAAATTCTTCGAGGATGCGTACTGGACCATCCTCAGCACTCTACAATCTCAAGACCAACTACGTGTCTATACACTCTGGTACACGAATTAATCAAACGCTAAATATATGATTTAGTTCGTGTTTTTTACTTTACAATAAGTTCGTTTAAAAATTTCAATTTAGTCTTTTAAATTTTAACATGTTTTATGATGTTATGAAGTTAGACTCTAGATAGTTTCATTTTATAGCCTGCCCTCCCCTTCGTATCTATTGATCGTTATCCATTTTTCTTCCATCCATCCTAGGTCCCCATAAAATTCTTTTTTCTTGAACTCATGAAATTGATTTCAAGTATTTTTTATAGGAAGTTAAAACGATTGTCTTCTACATGCTTTGTTCACCATAAAGGAATAACAGCTTACCCCGCTTTTGCAAGGCCTGATGGTCTACATAGACGATTTTGTGCCAAATCTAGCTGAAACTTCTGTATAGAATGTACTGTGGCGAGTCCTTCTCCTTCAGTTGTGGTGTAATTTTAGTGTGTTTGACATATTTGTCTTTTAAAGTTCTTAGAGTGCTTAGGGAGGAATATAACTATATAAGTACCTTTTTAAAAATATGTAGGTCCCCTCTTTGTGCTTAGGGAGTTTAATTTTAACAGTGTTTTAAACCTCATAAATGCTATCCCCTCTTTGTGCTCGTAATATCTTTGGTACAATACTGTGGGATCCATTTAGTTTGGTTGGAGAGAAATAATAGAACTTTTGCGGATGTCTCCAAATCTTACCACCACCTTGGGGATAACATTATAGTTTTAGCTTCCTCTTAGTCTTCTAAGTCTACTTTCTTTTATAACTACAATATCTCGTCCATTTCACTTAACTGGAGCGTTTTTACTTAGCCTCTTTATCTTTTGTACGCATGTTTCTCACATACTTGAATAAATCGTTCTAGTATCTTTTAAAAAAAATAGCGTTTTAAACCTATTAAAAGTTCTTTTTAAATTTTTAATAATCTAAAAGAGTTTAAAAGTTATTGAAAAAAGAAAAAACAGTTTACACTGGCTTTTAGATGAATCGTTTGATTAATTTTATTCTGAAAGTGTTTGCAAAATAAATACTTCACTTTACAACACTTTTTTCAATTGGCATCCTGAAATACATTTACTTTAGAGGGTTGACAGGAATTTTAGGGAGAGAATTAAACTACGTAAGAAAAAAAGTTTCTGATTCCTGCTATTAAAAAAGAAAGCTTTTAATTATTCTTTCTTCAAATTTTAAAGAAATAATAGGTGAATTTTTTACTTTTCCTACCTGGGAAAGCCATCCCTTAAATTGCTGTAAATTCATTAAATATCATGTCAAGTAAAGTTATCGCAAGTCAGTTTTATTTTCCTATCATATAATTGAAATTTTTCAAAATATAAGAAAAATTTTCGTCGCTGGAAAATTGTTTTCGTCGCATTTCCAGCGAGGAAAGTTTTTTTCGACACTGTTTTCGTCGCATTTATTTTCGTCGTTGTTTTGCAACGATAAATATTTTTTGTCGCTGGAAAAGCGACGAAAAAAACTTCGTCGCTAATTAATGTCGTTGAAGACTTTTTTGTTGTAGTGAATGAAGATTAAAATTCTTCTCAAAAAAACAAACAATGAAGATTAAATAAATAACACTTAAACATTAGGAACTCAAACATCTATCCAGCGGTAATTAAACAGCAACCACAGCCACTATTCCTTCATTCTATTTCACTTGTTGCGTTGAATTTATGGAACAGGTATCAAGTCATGGATCAACAGTTTTCCTAATTTGCCTTCCAAAAAGATCGTATTTGGAATTCCTTTCCATGGCTGGGCATGGAAACTCGCGGACGCTCACCGACACGACATCTTTTCGAAGGCCGAAGGACCAGCCGTAGGAGACAGTATTTCTTCGGACGGCCGAATCGACTACTCCAATATCAAGAAGTTTATAAAAGATAATCAGGCTGAGAATAAAAAAATGATGGGAGATATGTAA

mRNA sequence

ATGGCCCCAAGGCTTGTGCGCGGCGGTTACTGGTTCACCAGCACGGAGGCCGAGCTTCCTTCTGCTAATATTGATGCTACGTTTTTTAGCCATTTGTTTTGTGGATATGTTATTGTTGACCCTGAAACTTACAAGGTCACCATAGATTCCTCGGAGGTTATCCTAATCAATCAATTTAGTTCGACTGTCAGACTGAGCAATCCGAGAGTCAAAACTTTGCTATCCATCAGAGGAGATGGAGATGTACTCACTAAAATGGTTAGCGAGCGTGCAAATCGCGAAGCCTTTATTAATTCCTCGATTGAGGCAGCAATAGAGGGAAAGTTTAACGGCTTAGATCTCGAGTGGCTGTATCCATCTTCTCAAGATGAAATGGAAGGCTTTGAAAACGTTCTCATTGAGTGGCATAGCGCTATTGTTGAGGATGCTAAGAATTACCATAGACAACAGTTGATCCTTGTTGCTGCAGTCTCCAACTTGCCCATCGTGCGCCACAATATTCAGTATCCAATTGATACAATAATACAAACCTTGGATTGGGTTAACCTTATTTCTTATGATTTTTACACCCCAAATTCTTCGAGGATGCGTACTGGACCATCCTCAGCACTCTACAATCTCAAGACCAACTACGTGTCTATACACTCTGGTATCAAGTCATGGATCAACAGTTTTCCTAATTTGCCTTCCAAAAAGATCGTATTTGGAATTCCTTTCCATGGCTGGGCATGGAAACTCGCGGACGCTCACCGACACGACATCTTTTCGAAGGCCGAAGGACCAGCCGTAGGAGACAGTATTTCTTCGGACGGCCGAATCGACTACTCCAATATCAAGAAGTTTATAAAAGATAATCAGGCTGAGAATAAAAAAATGATGGGAGATATGTAA

Coding sequence (CDS)

ATGGCCCCAAGGCTTGTGCGCGGCGGTTACTGGTTCACCAGCACGGAGGCCGAGCTTCCTTCTGCTAATATTGATGCTACGTTTTTTAGCCATTTGTTTTGTGGATATGTTATTGTTGACCCTGAAACTTACAAGGTCACCATAGATTCCTCGGAGGTTATCCTAATCAATCAATTTAGTTCGACTGTCAGACTGAGCAATCCGAGAGTCAAAACTTTGCTATCCATCAGAGGAGATGGAGATGTACTCACTAAAATGGTTAGCGAGCGTGCAAATCGCGAAGCCTTTATTAATTCCTCGATTGAGGCAGCAATAGAGGGAAAGTTTAACGGCTTAGATCTCGAGTGGCTGTATCCATCTTCTCAAGATGAAATGGAAGGCTTTGAAAACGTTCTCATTGAGTGGCATAGCGCTATTGTTGAGGATGCTAAGAATTACCATAGACAACAGTTGATCCTTGTTGCTGCAGTCTCCAACTTGCCCATCGTGCGCCACAATATTCAGTATCCAATTGATACAATAATACAAACCTTGGATTGGGTTAACCTTATTTCTTATGATTTTTACACCCCAAATTCTTCGAGGATGCGTACTGGACCATCCTCAGCACTCTACAATCTCAAGACCAACTACGTGTCTATACACTCTGGTATCAAGTCATGGATCAACAGTTTTCCTAATTTGCCTTCCAAAAAGATCGTATTTGGAATTCCTTTCCATGGCTGGGCATGGAAACTCGCGGACGCTCACCGACACGACATCTTTTCGAAGGCCGAAGGACCAGCCGTAGGAGACAGTATTTCTTCGGACGGCCGAATCGACTACTCCAATATCAAGAAGTTTATAAAAGATAATCAGGCTGAGAATAAAAAAATGATGGGAGATATGTAA

Protein sequence

MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM

Homology

[Close]

BLAST of MC08g_new0624 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.7e-56
Identity = 125/303 (41.25%), Postives = 184/303 (60.73%), Query Frame = 0


Query: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60
           MA  +V+  YWF +  +E P  +ID++ F+HLFC +  ++ +T +VT+ S+     + F+
Sbjct: 23  MAQTVVKASYWFPA--SEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFT 82

Query: 61  STVRLSNPRVKTLLSIRG---DGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWL 120
            TV+  NP VKTLLSI G   D      M S   +R++FI+SSI  A    F+GLDL+W 
Sbjct: 83  QTVQRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWE 142

Query: 121 YPSSQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAV--SNLPIVRHNIQYPIDTII 180
           YPSS  EM  F  +L EW SA+V +A +  + +L+L AAV  SN     +++ YP+  + 
Sbjct: 143 YPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSN---NYYSVLYPVSAVA 202

Query: 181 QTLDWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVF 240
            +LDWVNL++YDFY P  SR+ TGP +AL++      S  +G +SWI +   LP+KK V 
Sbjct: 203 SSLDWVNLMAYDFYGPGWSRV-TGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVL 262

Query: 241 GIPFHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAE---NKKM 296
           G P++G+AW+L +A+ H  ++    P  G +IS DG I Y  I+KFI DN A    N  +
Sbjct: 263 GFPYYGYAWRLTNANSHSYYA----PTTGAAISPDGSIGYGQIRKFIVDNGATTVYNSTV 313

[Close]

BLAST of MC08g_new0624 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 3.2e-39
Identity = 99/290 (34.14%), Postives = 166/290 (57.24%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           VR  YW     ++  +++ID  +F+H++  +V   PE++ + I  S      ++   +  
Sbjct: 55  VRAAYW--PAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDG-IHN 114

Query: 66  SNPRVKTLLSIRGDGD---VLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQ 125
             PRV TLLSI G G+   + ++M S + NR +FINS+I  A + +FNGLDL+W +P  +
Sbjct: 115 IRPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 126 DEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAV---SNLPIVRHNIQ-YPIDTIIQTL 185
            +M     +L EW+ A+V +A    + +L+L +AV   S + ++ +  + YP+  I + L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 186 DWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIP 245
           DW + + +D+    +    TG ++ALY+  +N +S   GI SWI S   +P++K+V G+P
Sbjct: 235 DWASPMCFDY--NGAWANETGFNAALYDPNSN-ISTKYGIGSWIGS--GVPAEKLVMGLP 294

Query: 246 FHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAE 289
            +G AW+L D + H + +KA GPAV     +DG +DY  I  F KD  A+
Sbjct: 295 LYGRAWELKDPNDHGVGAKAVGPAV----DTDGSMDYDEILVFNKDTGAK 332

[Close]

BLAST of MC08g_new0624 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.6e-38
Identity = 97/289 (33.56%), Postives = 157/289 (54.33%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V+G YW  +     P   +D + F+H+F  ++  +  TYK+ I SS+++ +N F+ T + 
Sbjct: 35  VKGIYWLEN--PFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKT 94

Query: 66  SNPRVKTLLSIRGDG---DVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQ 125
            +P   TL SI G G    +L  + S+     AFINS+I+ A    F+G+DL+W +P + 
Sbjct: 95  KSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNT 154

Query: 126 DEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAV---SNLPIVRHNIQYPIDTIIQTLD 185
            EM     +L +W  AI ++     R  L+L AAV    N  I      YP+++I + LD
Sbjct: 155 KEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLD 214

Query: 186 WVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPF 245
           WVN++SY+   P S+  +TG  S  ++ K+N VS+ SG+ SWI+S   +  +K+V G+P 
Sbjct: 215 WVNVMSYELRGPRSN--KTGAPSGTFDPKSN-VSVVSGLLSWIHS--GVVPEKLVMGMPL 274

Query: 246 HGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAE 289
           +G +WKL D + H I      P+VG     +G + Y  +  F +   A+
Sbjct: 275 YGKSWKLRDPNVHGI----GAPSVGSGPGVNGLMAYFQVLDFNRQKSAK 312

[Close]

BLAST of MC08g_new0624 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.3e-34
Identity = 95/290 (32.76%), Postives = 162/290 (55.86%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           VR  YW    +   PS  ID  +F+H+   ++  +P ++K+ I  S +     F   +R 
Sbjct: 40  VRSAYWPAGDDFS-PSL-IDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 66  SNPRVKTLLSIRGDGD---VLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQ 125
            +P VKTLLSI G G    + +++ S + NRE FINS+IE A + +F+G+DL+W +P +Q
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 126 DEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVR----HNIQ-YPIDTIIQT 185
            +M     +  EW++A+  +AK   + +L+L +AV     VR    H  + YP   I + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 186 LDWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGI 245
           LDW + + +D++   +    T  ++ALY+ K+  +S + G+ SWI S   +  +K+V G+
Sbjct: 220 LDWASPMCFDYH--GTWDNNTDFNAALYDSKSE-ISTNFGLHSWIKS--GVRPEKLVMGL 279

Query: 246 PFHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 288
             +G AW+L D + + + ++A GPA      +DG ++Y+ I KF K + A
Sbjct: 280 ALYGRAWELKDPNVNGVGAEAVGPAT----DTDGSMNYNEILKFNKQSGA 318

[Close]

BLAST of MC08g_new0624 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 5.3e-34
Identity = 95/293 (32.42%), Postives = 153/293 (52.22%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSE----VILINQFSS 65
           V+G YW  +     P ++ID + F+H+F  +V  +  TYK+  +  +       +  F++
Sbjct: 37  VKGIYWIEN--PLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTTFTN 96

Query: 66  TVRLSNPRVKTLLSIRG---DGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLY 125
           T +   P + TLLSI G   +  +   + S+   R  FINS+I+ A    F+G+D +W +
Sbjct: 97  TFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEF 156

Query: 126 PSSQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAV---SNLPIVRHNIQYPIDTII 185
           P++  EM     +L +W  AI ++  +  R  L+L AAV    N  +      YP+D+I 
Sbjct: 157 PTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSIN 216

Query: 186 QTLDWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVF 245
           + LDWVN++SYD     S+   TG  S +++ K+N VS+ SG+ SWI     +  +KIV 
Sbjct: 217 KNLDWVNVMSYDLRGSGSN--VTGAPSGMFDSKSN-VSVVSGLFSWIRG--GVAPEKIVM 276

Query: 246 GIPFHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAE 289
           G+P +G +WKL D + H I +   GP  G     DG + Y  +  F K   A+
Sbjct: 277 GMPLYGKSWKLQDPNVHGIGAPNVGPGPG----VDGGMAYFQVVDFNKQMGAK 318

[Close]

BLAST of MC08g_new0624 vs. NCBI nr
Match: XP_022143178.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 597 bits (1540), Expect = 1.85e-215
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60
           MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS
Sbjct: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60

Query: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120
           STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS
Sbjct: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120

Query: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180
           SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW
Sbjct: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180

Query: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240
           VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH
Sbjct: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240

Query: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM 296
           GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM
Sbjct: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM 296

[Close]

BLAST of MC08g_new0624 vs. NCBI nr
Match: XP_022143191.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 442 bits (1138), Expect = 3.07e-153
Identity = 215/294 (73.13%), Postives = 248/294 (84.35%), Query Frame = 0


Query: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60
           M P  VR  YWF ST AELPSANIDA FF+HLFCGY IV+P+TY+VTIDSSE  +IN F+
Sbjct: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60

Query: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120
            TV LSNP VKTLLSI+GDG VL KM SE  NRE FI SSI+ A EG F+GLDL+WLYPS
Sbjct: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120

Query: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180
           SQ+EME FE++LIEWHSA+VEDA+NYHRQQLILVAAVSNLPIV HNIQYPIDTIIQTLDW
Sbjct: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180

Query: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240
           VNL SYDFYTP SS   TGPSSALYN KT+ +S++SGI++WI  +PNLPS++IVFGIPFH
Sbjct: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240

Query: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMG 294
           GWAWKLAD  +HD+FS+A+G A+G  ISSDG+I YSNIKKFI+ N+A+N K  G
Sbjct: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDG 294

[Close]

BLAST of MC08g_new0624 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 363 bits (933), Expect = 4.42e-122
Identity = 178/284 (62.68%), Postives = 215/284 (75.70%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V GGYW +ST  +LP +NIDA  F+HLFCG+  +D  +Y+VT+ +S+  LINQFSSTVR+
Sbjct: 11  VNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTVRM 70

Query: 66  SNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEM 125
           SNP VKTLLSIRGD DV  KM SE   R+ FI SSIE A +GKFNGLDL+WLYPSS++EM
Sbjct: 71  SNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEEM 130

Query: 126 EGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLIS 185
           + FE +L  W  A V+DAK YHR Q ILVA+VSNLP V  +I YP++ I Q LDWVNLIS
Sbjct: 131 QNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLIS 190

Query: 186 YDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWK 245
           YDFYTP SS   TGPSSAL+N     +S + GI +WI S  NLP K+IVFGIPFHGWAWK
Sbjct: 191 YDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAWK 250

Query: 246 LADAHRHDIFSKAEGPAVGDS-ISSDGRIDYSNIKKFIKDNQAE 288
           LA+  +HD+FSKA GPA GD  IS +G+ID SN+K+FIK N A+
Sbjct: 251 LANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQ 294

[Close]

BLAST of MC08g_new0624 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 354 bits (908), Expect = 2.56e-118
Identity = 169/282 (59.93%), Postives = 215/282 (76.24%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V  GYW  ST+  LP+ANIDAT F+HLF GY  ++ +TY+VTID S +ILI+QFSSTV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70

Query: 66  SNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEM 125
           SNP VKTLLSI  D  +   M ++  NR+AFI SSI AA  G F+GLDL+WLYPSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 126 EGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLIS 185
           + F+ ++  WH A+V+DA +    QL+LVA VSNLP ++ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 186 YDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWK 245
           YDFYTP+SSR  TGPSSA +N K N +S + GI+SW+ S  NLP KKIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 246 LADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 287
           L ++H+H +F+KA+G A G+ I   G+I YS+++KFI+DNQA
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQA 292

[Close]

BLAST of MC08g_new0624 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 353 bits (906), Expect = 5.15e-118
Identity = 169/282 (59.93%), Postives = 215/282 (76.24%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V  GYW  ST+  LP+ANIDAT F+HLF GY  ++ +TY+VTID S++ILI+QFSSTV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILIDQFSSTVKL 70

Query: 66  SNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEM 125
           SNP VKTLLSI  D  +   M ++  NR+AFI SSI AA  G F+GLDL+WLYPSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 126 EGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLIS 185
           + F+ ++  WH A+V+DA +    QL+LVA VSNLP ++ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 186 YDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWK 245
           YDFYTP+SSR  TGPSSA +N K N +S + GI+SW  S  NLP KKIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 246 LADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 287
           L ++H+H +F+KA+G A G+ I   G+I YS+++KFI+DNQA
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQA 292

[Close]

BLAST of MC08g_new0624 vs. ExPASy TrEMBL
Match: A0A6J1CQ27 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111013111 PE=4 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 8.96e-216
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60
           MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS
Sbjct: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60

Query: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120
           STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS
Sbjct: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120

Query: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180
           SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW
Sbjct: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180

Query: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240
           VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH
Sbjct: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240

Query: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM 296
           GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM
Sbjct: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMGDM 296

[Close]

BLAST of MC08g_new0624 vs. ExPASy TrEMBL
Match: A0A6J1CPI6 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 1.49e-153
Identity = 215/294 (73.13%), Postives = 248/294 (84.35%), Query Frame = 0


Query: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60
           M P  VR  YWF ST AELPSANIDA FF+HLFCGY IV+P+TY+VTIDSSE  +IN F+
Sbjct: 1   MDPSFVRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFT 60

Query: 61  STVRLSNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPS 120
            TV LSNP VKTLLSI+GDG VL KM SE  NRE FI SSI+ A EG F+GLDL+WLYPS
Sbjct: 61  LTVSLSNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPS 120

Query: 121 SQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDW 180
           SQ+EME FE++LIEWHSA+VEDA+NYHRQQLILVAAVSNLPIV HNIQYPIDTIIQTLDW
Sbjct: 121 SQEEMENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDW 180

Query: 181 VNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFH 240
           VNL SYDFYTP SS   TGPSSALYN KT+ +S++SGI++WI  +PNLPS++IVFGIPFH
Sbjct: 181 VNLFSYDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFH 240

Query: 241 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAENKKMMG 294
           GWAWKLAD  +HD+FS+A+G A+G  ISSDG+I YSNIKKFI+ N+A+N K  G
Sbjct: 241 GWAWKLADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDG 294

[Close]

BLAST of MC08g_new0624 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 363 bits (933), Expect = 2.14e-122
Identity = 178/284 (62.68%), Postives = 215/284 (75.70%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V GGYW +ST  +LP +NIDA  F+HLFCG+  +D  +Y+VT+ +S+  LINQFSSTVR+
Sbjct: 11  VNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTVRM 70

Query: 66  SNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEM 125
           SNP VKTLLSIRGD DV  KM SE   R+ FI SSIE A +GKFNGLDL+WLYPSS++EM
Sbjct: 71  SNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEEM 130

Query: 126 EGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLIS 185
           + FE +L  W  A V+DAK YHR Q ILVA+VSNLP V  +I YP++ I Q LDWVNLIS
Sbjct: 131 QNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLIS 190

Query: 186 YDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWK 245
           YDFYTP SS   TGPSSAL+N     +S + GI +WI S  NLP K+IVFGIPFHGWAWK
Sbjct: 191 YDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAWK 250

Query: 246 LADAHRHDIFSKAEGPAVGDS-ISSDGRIDYSNIKKFIKDNQAE 288
           LA+  +HD+FSKA GPA GD  IS +G+ID SN+K+FIK N A+
Sbjct: 251 LANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQ 294

[Close]

BLAST of MC08g_new0624 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 352 bits (904), Expect = 5.01e-118
Identity = 169/282 (59.93%), Postives = 214/282 (75.89%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V  GYW  ST+  LP+ANIDAT F+HLF GY  ++  TY+VT+D S++ILI+QFSSTV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSSTVKL 70

Query: 66  SNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEM 125
           SNP VKTLLSI  D  V   M ++  NR+AFI SSI AA  G F+GLDL+WLYPSSQD+M
Sbjct: 71  SNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 126 EGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLIS 185
           + F+ ++  WH A+V+DA +    QL+LVA VSNLP ++ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 186 YDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWK 245
           YDFYTP+SSR  TGPSSA +N K N +S + GI+SW  S  NLP KKIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 246 LADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 287
           L ++H+H +F+KA+G A G+ I   G+I YS+++KFI+DNQA
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQA 292

[Close]

BLAST of MC08g_new0624 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 352 bits (903), Expect = 7.11e-118
Identity = 169/282 (59.93%), Postives = 215/282 (76.24%), Query Frame = 0


Query: 6   VRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVRL 65
           V  GYW  ST+  LP+ANIDAT F+HLF GY  ++ +TY+VT+D S++ILI+QFSSTV+L
Sbjct: 11  VNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSSTVKL 70

Query: 66  SNPRVKTLLSIRGDGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSSQDEM 125
           SNP VKTLLSI GD  V   M ++  NR+AFI SSI AA  G F+GLDL+WLYPSSQD+M
Sbjct: 71  SNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 126 EGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWVNLIS 185
             F+ ++  WH A+V+DA +    QL+LVA VSNLP +++NIQYP D I Q LDWVNL+S
Sbjct: 131 ANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWVNLLS 190

Query: 186 YDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIPFHGWAWK 245
           YDFYTP+S R  TGPSSA +N K N +S + GI+SW  S  NL  KKIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHGWAWK 250

Query: 246 LADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 287
           LA++H+H +F+KA+G A G+ I   G+I YS+++KFI+DNQA
Sbjct: 251 LANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQA 292

[Close]

BLAST of MC08g_new0624 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 219.9 bits (559), Expect = 2.7e-57
Identity = 125/303 (41.25%), Postives = 184/303 (60.73%), Query Frame = 0


Query: 1   MAPRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFS 60
           MA  +V+  YWF +  +E P  +ID++ F+HLFC +  ++ +T +VT+ S+     + F+
Sbjct: 23  MAQTVVKASYWFPA--SEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFT 82

Query: 61  STVRLSNPRVKTLLSIRG---DGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWL 120
            TV+  NP VKTLLSI G   D      M S   +R++FI+SSI  A    F+GLDL+W 
Sbjct: 83  QTVQRRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWE 142

Query: 121 YPSSQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAV--SNLPIVRHNIQYPIDTII 180
           YPSS  EM  F  +L EW SA+V +A +  + +L+L AAV  SN     +++ YP+  + 
Sbjct: 143 YPSSATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSN---NYYSVLYPVSAVA 202

Query: 181 QTLDWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVF 240
            +LDWVNL++YDFY P  SR+ TGP +AL++      S  +G +SWI +   LP+KK V 
Sbjct: 203 SSLDWVNLMAYDFYGPGWSRV-TGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVL 262

Query: 241 GIPFHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQAE---NKKM 296
           G P++G+AW+L +A+ H  ++    P  G +IS DG I Y  I+KFI DN A    N  +
Sbjct: 263 GFPYYGYAWRLTNANSHSYYA----PTTGAAISPDGSIGYGQIRKFIVDNGATTVYNSTV 313

[Close]

BLAST of MC08g_new0624 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 209.9 bits (533), Expect = 2.8e-54
Identity = 116/289 (40.14%), Postives = 171/289 (59.17%), Query Frame = 0


Query: 2   APRLVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSS 61
           A  +V+  YWF   E+E P A ID++ F+HLFC +  ++  TY+V + S      + F+ 
Sbjct: 22  AQTVVKATYWF--AESESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQ 81

Query: 62  TVRLSNPRVKTLLSIRGD---GDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLY 121
           TVR  NP VKTLLSI GD         M S   +R+ FI+SSI+ A    F+GLDL W Y
Sbjct: 82  TVRRRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKY 141

Query: 122 PSSQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTL 181
           PS   EM+ F  +L EW  A+  +A++  + +L+L AAV       +++ +P++ +  +L
Sbjct: 142 PSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSL 201

Query: 182 DWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIP 241
           DWVNL++YDFY   SSR+   P + LY+  T   S  +G+++W  +   LP+KK V G P
Sbjct: 202 DWVNLVAYDFYESGSSRVTCSP-APLYDPITTGPSGDAGVRAWTQA--GLPAKKAVLGFP 261

Query: 242 FHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 288
            +G+AW L DA  H+ ++ + GPA    IS DG I Y  I++FI DN+A
Sbjct: 262 LYGYAWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKA 300

[Close]

BLAST of MC08g_new0624 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 204.1 bits (518), Expect = 1.5e-52
Identity = 119/296 (40.20%), Postives = 173/296 (58.45%), Query Frame = 0


Query: 5   LVRGGYWFTSTEAE--------LPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILI 64
           +V+  YWF   +++         PS+ ID+T F+HLFC +  VD  T++VTI ++     
Sbjct: 13  IVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQF 72

Query: 65  NQFSSTVRLSNPRVKTLLSIRG---DGDVLTKMVSERANREAFINSSIEAAIEGKFNGLD 124
           + F+ TV+  N  V+TLLSI G   D  VL  M S   NR+AFI+SSI+ A +  F GLD
Sbjct: 73  SSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLD 132

Query: 125 LEWLYPSSQDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDT 184
           L W YPS+  EM  F  +L EW +A+VE++   ++  L+L AAV   P     ++YP+  
Sbjct: 133 LAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSP-QYDGVEYPVKA 192

Query: 185 IIQTLDWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYV--SIHSGIKSWINSFPNLPSK 244
           I   LD+VN+++YDFY P  S + TGP +AL++  +N    S +SG++ W++    LP K
Sbjct: 193 IADNLDFVNIMAYDFYGPGWSPV-TGPPAALFHDPSNPAGRSGNSGLRKWLDE-AKLPPK 252

Query: 245 KIVFGIPFHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 288
           K V G P+ GWAW L DA  +   +  +G A    IS DG I Y+ I+ +I DN A
Sbjct: 253 KAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGA 301

[Close]

BLAST of MC08g_new0624 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-52
Identity = 117/287 (40.77%), Postives = 169/287 (58.89%), Query Frame = 0


Query: 5   LVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVR 64
           +V+  YW    E + P+ NID+T F+HLFC +  VD  T++VTI ++    ++ F+ TV+
Sbjct: 13  IVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVK 72

Query: 65  LSNPRVKTLLSIRG---DGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSS 124
             N  V+TLLSI G   D  VL  M S   NR+AFI+SSI+ A +  F GLDL W YPS+
Sbjct: 73  DKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSN 132

Query: 125 QDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAVSNLPIVRHNIQYPIDTIIQTLDWV 184
             EM  F  ++ EW +A+VE++   ++  L+L AAV   P   +  +YP+  I   LD+V
Sbjct: 133 DVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDY-YGEEYPVQAIADNLDFV 192

Query: 185 NLISYDFYTPNSSRMRTGPSSALYNLKTNY-VSIHSGIKSWINSFPNLPSKKIVFGIPFH 244
           N+++YDFY P  S + TGP +AL++       S  SG+  W+ +   LP+KK V G  + 
Sbjct: 193 NIMAYDFYGPGWSPV-TGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKAVLGFSYC 252

Query: 245 GWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA 288
           GWAW L DA  +   +  +G A    ISSDG I Y+ I+ +I DN A
Sbjct: 253 GWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGA 291

[Close]

BLAST of MC08g_new0624 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 201.8 bits (512), Expect = 7.5e-52
Identity = 118/300 (39.33%), Postives = 174/300 (58.00%), Query Frame = 0


Query: 5   LVRGGYWFTSTEAELPSANIDATFFSHLFCGYVIVDPETYKVTIDSSEVILINQFSSTVR 64
           +V+  YWF +T  + P+ +ID++ F+HLFC +  ++ E+Y++TI +      + F+ TV+
Sbjct: 6   VVKASYWFPAT--DFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQ 65

Query: 65  LSNPRVKTLLSIRG---DGDVLTKMVSERANREAFINSSIEAAIEGKFNGLDLEWLYPSS 124
             NP VKTLLSI G   D D    M S   +R +FI S+I  A    F+GLDL+W YP +
Sbjct: 66  QRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRN 125

Query: 125 QDEMEGFENVLIEWHSAIVEDAKNYHRQQLILVAAV---SNLPIVRHNIQYPIDTIIQTL 184
           ++EM  F  +L EW SA+  ++ +     LIL AAV   SN       + YP+  I  +L
Sbjct: 126 EEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSNY----QGVPYPVLAISNSL 185

Query: 185 DWVNLISYDFYTPNSSRMRTGPSSALYNLKTNYVSIHSGIKSWINSFPNLPSKKIVFGIP 244
           DW+NL++YDFY P  S + TGP ++LY L T+  S  SG++ W  +   LP+KK V G P
Sbjct: 186 DWINLMAYDFYGPGWSTV-TGPPASLY-LPTDGRSGDSGVRDWTEA--GLPAKKAVLGFP 245

Query: 245 FHGWAWKLADAHRHDIFSKAEGPAVGDSISSDGRIDYSNIKKFIKDNQA---ENKKMMGD 296
           ++GWAW LAD   +   +   GPA+ D    DG I Y  ++ +I DN A    +  M+GD
Sbjct: 246 YYGWAWTLADPDVNGYDANTTGPAISD----DGEISYRQLQTWIVDNGATKVHDDMMVGD 291

The following BLAST results are available for this feature:

BLAST of MC08g_new0624 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	3.7e-56	41.25	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
A0A1B1J8Z2	3.2e-39	34.14	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]
A0A072UR65	1.6e-38	33.56	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
A0A072VEP0	5.3e-34	32.76	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]
U5N4E3	5.3e-34	32.42	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]

BLAST of MC08g_new0624 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143178.1	1.85e-215	100.00	chitinase-3-like protein 2 [Momordica charantia]	[more]
XP_022143191.1	3.07e-153	73.13	chitotriosidase-1-like [Momordica charantia]	[more]
XP_022159350.1	4.42e-122	62.68	chitinase-3-like protein 2 [Momordica charantia]	[more]
KAG7030350.1	2.56e-118	59.93	Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6599362.1	5.15e-118	59.93	Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1...	[more]

BLAST of MC08g_new0624 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQ27	8.96e-216	100.00	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111013111 PE=4 S...	[more]
A0A6J1CPI6	1.49e-153	73.13	chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1	[more]
A0A6J1DYG4	2.14e-122	62.68	chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S...	[more]
A0A6J1G2P4	5.01e-118	59.93	acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE...	[more]
A0A6J1KB82	7.11e-118	59.93	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1	[more]

BLAST of MC08g_new0624 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	2.7e-57	41.25	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	2.8e-54	40.14	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19760.1	1.5e-52	40.20	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	2.0e-52	40.77	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	7.5e-52	39.33	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 5..295 e-value: 4.6E-33 score: 125.9
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 9..288 e-value: 8.2E-47 score: 160.4
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 5..296 score: 35.080803
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 239..289 e-value: 3.2E-61 score: 209.4
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..238 e-value: 3.2E-61 score: 209.4
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 3..289
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 3..289
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 7..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0624.1	MC08g_new0624.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0016787	hydrolase activity