MC08g_new0614 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g_new0614
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionANK_REP_REGION domain-containing protein
LocationMC08: 32291337 .. 32292775 (+)
RNA-Seq ExpressionMC08g_new0614
SyntenyMC08g_new0614
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAGAAGTGCAAGATGAATTAGGACTGACTCCTCTTCATTACGCTGCATTTTATGGAAGGACTGAAGCAATAGAGCTATTTCTTGAAAAGAAAAGCTCTGCAATGTACATTTTAGATGCCAATGGAGAGTCTGCTCTACACATTGCTGCCTCTAGAGGCCATATCAATGCAGTGAAACAGATTTTAGACTCTTGTCCAGATTCCTGCTATCTGGTTGATAAGAAAGGTCGAACCGCGCTGCATGCTGCAGTCATGGGCGACCAAAGAAAAGCCATCAAATTCATCTTACTCGAGCCGAGGCTTGCTCCACTCATTAACAAGCCAGATGACAATGGAAACATACCCCTGCATCTCGCAGCGCTCTTCGAATTATACGATGTCGTTGAGATTCTTTCCAATAGTAGCCATGTGAATTTGAACTTAAAAAACAAGGAGTTCTTGACCGCCCTTGATGTTTACAATAAACACGATGAGGTACTTTAAGGATTTGTTTTGAACTATATTATTATAGTGTTAAAAGACAACGGTATCCTAAATACCCAAATATCTCTATAATAGCATGATATTGTCTGTTTTGAACCTTCACCGATTTACTTTTGGTTTTACCTAAAAGACCTCATATCAATGGAAATAATTGTCCTCATTTATATTCTTAACTGTTAGATATCTAATATATGAATTAGATTTACCCAATTCATTAGCTTAAGTTTTTGGGTTGAGTAATGATTTATTCATGCTTAATATGGTATCAGAGTAGCAATATATGAATTGAATTTACTCAATCCATTAACTTAAGTTTTTAGGTTGAGTGGTGGTTTATTCGTACTTAATATCAGCTAATGTAGAATTTTGTTTGCACACTGTGTAACCTCAACATATAGCTCATATATCTCAGGGTCTTTTTTCTTTTCTAGGAAGGACTAAGGGCCGCTGTAACTCGCTACCTCCTCGACAGCTCGACGGGAACAATGACAATGCAGCAGTTGGCTGAGAAAGAGATAAAAAAGATGAATCAAGAAGTGAAGATAGACATTATTCCTCGGAGTGGTTCTTCAGGGAGTAGTGAAAAAAGAGAGAGTTTATTCACAGACAAGAAAACTGCACTGGAAGTGAATCTACTGGTTGCAACACTAGTGGCCACAGTGACATTTGCAGCAGGATTCTCAATGCCTGGTGGAAACTTTCAGGACATTGGAAATGCAATTCTTACTACAAAAGCAGCTTTTCAAGTGTTTGTGATTTTCAACACAGTGGCATTTTGCAGCTCAGTTTTTGCAGTGCTTCTTCAGTTCCACACTTCACTTACAGACCACCACCAAAGAGTGAGGTACATGGGAATTGCTTCTTTCTTCACAACTGTTGCGATTCTCGCGATGGTCATTGCGTTTGCTGCAGGAACTCATGTGGTGCTTACGAGATCGATGAAATTTCGCTAA

mRNA sequence

ATGATAGAAGTGCAAGATGAATTAGGACTGACTCCTCTTCATTACGCTGCATTTTATGGAAGGACTGAAGCAATAGAGCTATTTCTTGAAAAGAAAAGCTCTGCAATGTACATTTTAGATGCCAATGGAGAGTCTGCTCTACACATTGCTGCCTCTAGAGGCCATATCAATGCAGTGAAACAGATTTTAGACTCTTGTCCAGATTCCTGCTATCTGGTTGATAAGAAAGGTCGAACCGCGCTGCATGCTGCAGTCATGGGCGACCAAAGAAAAGCCATCAAATTCATCTTACTCGAGCCGAGGCTTGCTCCACTCATTAACAAGCCAGATGACAATGGAAACATACCCCTGCATCTCGCAGCGCTCTTCGAATTATACGATGTCGTTGAGATTCTTTCCAATAGTAGCCATGTGAATTTGAACTTAAAAAACAAGGAGTTCTTGACCGCCCTTGATGTTTACAATAAACACGATGAGGAAGGACTAAGGGCCGCTGTAACTCGCTACCTCCTCGACAGCTCGACGGGAACAATGACAATGCAGCAGTTGGCTGAGAAAGAGATAAAAAAGATGAATCAAGAAGTGAAGATAGACATTATTCCTCGGAGTGGTTCTTCAGGGAGTAGTGAAAAAAGAGAGAGTTTATTCACAGACAAGAAAACTGCACTGGAAGTGAATCTACTGGTTGCAACACTAGTGGCCACAGTGACATTTGCAGCAGGATTCTCAATGCCTGGTGGAAACTTTCAGGACATTGGAAATGCAATTCTTACTACAAAAGCAGCTTTTCAAGTGTTTGTGATTTTCAACACAGTGGCATTTTGCAGCTCAGTTTTTGCAGTGCTTCTTCAGTTCCACACTTCACTTACAGACCACCACCAAAGAGTGAGGTACATGGGAATTGCTTCTTTCTTCACAACTGTTGCGATTCTCGCGATGGTCATTGCGTTTGCTGCAGGAACTCATGTGGTGCTTACGAGATCGATGAAATTTCGCTAA

Coding sequence (CDS)

ATGATAGAAGTGCAAGATGAATTAGGACTGACTCCTCTTCATTACGCTGCATTTTATGGAAGGACTGAAGCAATAGAGCTATTTCTTGAAAAGAAAAGCTCTGCAATGTACATTTTAGATGCCAATGGAGAGTCTGCTCTACACATTGCTGCCTCTAGAGGCCATATCAATGCAGTGAAACAGATTTTAGACTCTTGTCCAGATTCCTGCTATCTGGTTGATAAGAAAGGTCGAACCGCGCTGCATGCTGCAGTCATGGGCGACCAAAGAAAAGCCATCAAATTCATCTTACTCGAGCCGAGGCTTGCTCCACTCATTAACAAGCCAGATGACAATGGAAACATACCCCTGCATCTCGCAGCGCTCTTCGAATTATACGATGTCGTTGAGATTCTTTCCAATAGTAGCCATGTGAATTTGAACTTAAAAAACAAGGAGTTCTTGACCGCCCTTGATGTTTACAATAAACACGATGAGGAAGGACTAAGGGCCGCTGTAACTCGCTACCTCCTCGACAGCTCGACGGGAACAATGACAATGCAGCAGTTGGCTGAGAAAGAGATAAAAAAGATGAATCAAGAAGTGAAGATAGACATTATTCCTCGGAGTGGTTCTTCAGGGAGTAGTGAAAAAAGAGAGAGTTTATTCACAGACAAGAAAACTGCACTGGAAGTGAATCTACTGGTTGCAACACTAGTGGCCACAGTGACATTTGCAGCAGGATTCTCAATGCCTGGTGGAAACTTTCAGGACATTGGAAATGCAATTCTTACTACAAAAGCAGCTTTTCAAGTGTTTGTGATTTTCAACACAGTGGCATTTTGCAGCTCAGTTTTTGCAGTGCTTCTTCAGTTCCACACTTCACTTACAGACCACCACCAAAGAGTGAGGTACATGGGAATTGCTTCTTTCTTCACAACTGTTGCGATTCTCGCGATGGTCATTGCGTTTGCTGCAGGAACTCATGTGGTGCTTACGAGATCGATGAAATTTCGCTAA

Protein sequence

MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVKQILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLAALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTMQQLAEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDKKTALEVNLLVATLVATVTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKFR
Homology
BLAST of MC08g_new0614 vs. ExPASy Swiss-Prot
Match: Q9C7A2 (Ankyrin repeat-containing protein ITN1 OS=Arabidopsis thaliana OX=3702 GN=ITN1 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-28
Identity = 97/332 (29.22%), Postives = 172/332 (51.81%), Query Frame = 0

Query: 11  TPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVKQILDSCPDSC 70
           TPL  AA  G TE +   L K  + + I  +N ++ALH+AA +GH+  +K +L   P   
Sbjct: 200 TPLVSAAMRGHTEVVNQLLSKAGNLLEISRSNNKNALHLAARQGHVEVIKALLSKDPQLA 259

Query: 71  YLVDKKGRTALHAAVMGDQRKAIKFIL-LEPRLAPLINKPDDNGNIPLHLAALFELYDVV 130
             +DKKG+TALH AV G   + +K +L  +P    ++ +PD + N  LH+A   +  ++V
Sbjct: 260 RRIDKKGQTALHMAVKGQSSEVVKLLLDADP---AIVMQPDKSCNTALHVATRKKRAEIV 319

Query: 131 EILSNSSHVNLNLKNKEFLTALDVYNKHDEEGL-RAAVTRYLLD--SSTGTMTMQQLAE- 190
           E+L +    N N   ++  TALD+      EGL  +  + Y+ +  + +G +   +L + 
Sbjct: 320 ELLLSLPDTNANTLTRDHKTALDI-----AEGLPLSEESSYIKECLARSGALRANELNQP 379

Query: 191 -KEIKKMNQEVKIDI---------IPRSGSSGSSEKRESLFTDKKTALEVNLLVATLVAT 250
             E++    ++K D+           ++  + S E R+        A     +VA L AT
Sbjct: 380 RDELRSTVTQIKNDVHIQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLFAT 439

Query: 251 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 310
           V FAA F++PGG+  D G+A++  +A+F++F IFN +A  +S+  V++Q      +    
Sbjct: 440 VAFAAIFTVPGGDNND-GSAVVVGRASFKIFFIFNALALFTSLAVVVVQITLVRGETKAE 499

Query: 311 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTR 328
            R + + +    +A +   +AF A +++V+ R
Sbjct: 500 KRVVEVINKLMWLASMCTSVAFLASSYIVVGR 522

BLAST of MC08g_new0614 vs. ExPASy Swiss-Prot
Match: Q8LPS2 (Protein ACCELERATED CELL DEATH 6 OS=Arabidopsis thaliana OX=3702 GN=ACD6 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.9e-24
Identity = 97/333 (29.13%), Postives = 151/333 (45.35%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           +++ QDE G T L Y A  G  + +   L + +  +Y+ D +G   +H AA   H   +K
Sbjct: 288 LMDEQDEDGRTCLSYGASIGYYKGLCNILNRSTKGVYVCDQDGSFPIHSAAKNEHYEIIK 347

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           + +  CP S YL+++ G+  LH A   +       ++ +     L    D +GN PLHLA
Sbjct: 348 EFIKRCPASKYLLNRLGQNILHVAAKNEASLTAYMLMHDKDTKHLGVGQDVDGNTPLHLA 407

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVY------NKHDEEGLRAAVTRYLLDSS 180
            +   +D +  L++ +H  L L+NK  L A D+       N    E    A+  Y + SS
Sbjct: 408 VMNWDFDSITCLASRNHEILKLRNKSGLRARDIAESEVKPNYIFHERWTLALLLYAIHSS 467

Query: 181 TGTMTMQQLAEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDKKTALEVN--LLVATL 240
            G  +++ L         Q V +D                    KK    VN  L+VA L
Sbjct: 468 -GFESVKSLT-------IQSVPLD-------------------PKKNRHYVNALLVVAAL 527

Query: 241 VATVTFAAGFSMPGGNFQD-----IGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHT 300
           VATVTFAAGF++PGG   D     +G A L T     +F++F+ +A  SSV  +      
Sbjct: 528 VATVTFAAGFTIPGGYISDSKKPNLGRATLATNPTLFIFLLFDILAMQSSVATICTLIWA 587

Query: 301 SLTDHHQRVRYMGIASFFTTVAILAMVIAFAAG 321
            L D    ++ + +A      ++L M +AF  G
Sbjct: 588 QLGDLALILKSLHVALPLLLFSLLCMPVAFLFG 593

BLAST of MC08g_new0614 vs. ExPASy Swiss-Prot
Match: A2CIR5 (Ankyrin repeat-containing protein NPR4 OS=Oryza sativa subsp. japonica OX=39947 GN=NPR4 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.2e-24
Identity = 94/334 (28.14%), Postives = 163/334 (48.80%), Query Frame = 0

Query: 11  TPLHYAAFYGRTEAIELFLEKKSSAMY-ILDANGESALHIAASRGHINAVKQILDSCPDS 70
           +PL  AA  G TE ++L LE     +  +   NG+++LH AA +GH+  VK +L+  P  
Sbjct: 191 SPLISAATRGHTEVVKLLLELDDFGLVEMAKDNGKNSLHFAARQGHVEIVKALLEKDPQL 250

Query: 71  CYLVDKKGRTALHAAVMGDQRKAIKFIL-LEPRLAPLINKPDDNGNIPLHLAALFELYDV 130
               DKKG+TALH AV G     ++ ++  +P +  L   PD NGN  LH+A   +  ++
Sbjct: 251 ARRNDKKGQTALHMAVKGTNCDVLRALVDADPAIVML---PDKNGNTALHVATRKKRAEI 310

Query: 131 VEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTMQQLAE--K 190
           V +L      ++N   ++  TA D+          + +   L  S  G +  ++L +   
Sbjct: 311 VAVLLRLPDTHVNALTRDHKTAYDIAEALPLCEESSEIKDIL--SQHGALRSRELNQPRD 370

Query: 191 EIKKMNQEVKIDIIPRSGSSGSSEK---------RESLFTDKKTALEVNLLVATLVATVT 250
           E++K   E+K D+  +   +  + K         R+        A     +VA L ATV 
Sbjct: 371 ELRKTVTEIKKDVHTQLEQTRKTNKNVHGIAKELRKLHREGINNATNSVTVVAVLFATVA 430

Query: 251 FAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQRVR 310
           FAA F++PGGN  + G A++   A+F++F IFN +A  +S+  V++Q      +     +
Sbjct: 431 FAAIFTVPGGNANN-GVAVVVQAASFRIFFIFNAIALFTSLAVVVVQITVVRGETKSERK 490

Query: 311 YMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 332
            + + +    +A +   I+F A  ++VL R  ++
Sbjct: 491 VVEVINKLMWLASVCTTISFIASCYIVLGRHFQW 518

BLAST of MC08g_new0614 vs. ExPASy Swiss-Prot
Match: Q6AWW5 (Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=At5g02620 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 6.1e-23
Identity = 95/347 (27.38%), Postives = 166/347 (47.84%), Query Frame = 0

Query: 6   DELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVKQILDS 65
           D    T LH AA  G  E +   L+K      I  +NG++ALH AA  GH   VK++++ 
Sbjct: 122 DSSKTTALHTAASQGHGEIVCFLLDKGVDLAAIARSNGKTALHSAARNGHTVIVKKLIEK 181

Query: 66  CPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLAALFEL 125
                  VDKKG+TALH AV G Q   I  +L+E     LIN  D+ GN PLH+A     
Sbjct: 182 KAGMVTRVDKKGQTALHMAVKG-QNTEIVDVLMEAD-GSLINSADNKGNTPLHIAVRKNR 241

Query: 126 YDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTMQQL-- 185
            ++V+ +     V+    NK   TALD+  K     +   + +  + ++      +++  
Sbjct: 242 AEIVQTVLKYCEVSRVAVNKSGETALDIAEKTGLHEIVPLLQKIGMQNARSIKPAEKVEP 301

Query: 186 --AEKEIKKMNQEVKIDI---IPRSGSS-----GSSEKRESLFTD-KKTALEVNLLVATL 245
             + +++K+   E+  ++   + ++G +     G +++   + T+    A+    LVA L
Sbjct: 302 SGSSRKLKETVSEIGHEVHTQLEQTGRTRREIQGIAKRVNKMHTEGLNNAINSTTLVAIL 361

Query: 246 VATVTFAAGFSMPGGNFQD---------IGNAILTTKAAFQVFVIFNTVAFCSSVFAVLL 305
           +ATV FAA F++PG    D         +G A    +  F +FV+F++ A   S+  V++
Sbjct: 362 IATVAFAAIFNVPGQYTDDPKDVPPGYSLGEARAAPRPEFLIFVVFDSFALFISLAVVVV 421

Query: 306 QFHTSLTDHHQRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMK 331
           Q    + +   + + M I +    +A + + +AF + + VV+    K
Sbjct: 422 QTSVVVIERRAKKQMMAIINKLMWMACIMISVAFVSLSFVVVGEKEK 466

BLAST of MC08g_new0614 vs. ExPASy Swiss-Prot
Match: Q9ZU96 (Ankyrin repeat-containing protein At2g01680 OS=Arabidopsis thaliana OX=3702 GN=At2g01680 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.6e-15
Identity = 75/333 (22.52%), Postives = 145/333 (43.54%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           +  + D    +PL+ AA     E +   L+   S   I+  NG+++LH A   G +  VK
Sbjct: 120 LCRICDASNTSPLYAAAVQDHLEIVNAMLDVDPSCAMIVRKNGKTSLHTAGRYGLLRIVK 179

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
            +++       + DKKG+TALH AV G   + ++ IL       ++N+ D  GN  LH+A
Sbjct: 180 ALIEKDAAIVGVKDKKGQTALHMAVKGRSLEVVEEILQAD--YTILNERDRKGNTALHIA 239

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSST----- 180
                  +  +L   + + +N  N +  TA+D+ +K         +   L+++       
Sbjct: 240 TRKARPQITSLLLTFTAIEVNAINNQKETAMDLADKLQYSESALEINEALVEAGAKHGRF 299

Query: 181 -GTMTMQQLAEKEIKKMNQEVKIDIIPRSGS----SGSSEKRESLFTDK-KTALEVNLLV 240
            G     +  ++ +  +  EV+  ++    +    SG +++   L  +  +       +V
Sbjct: 300 IGREDEARALKRAVSDIKHEVQSQLLQNEKTNRRVSGIAKELRKLHREAVQNTTNSITVV 359

Query: 241 ATLVATVTFAAGFSMPGGNFQD---IGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFH 300
           A L A++ F A F++PG  F +   +G A +  +  F+VF + N  +   S+  V++Q  
Sbjct: 360 AVLFASIAFLAIFNLPGQYFTEGSHVGQANIAGRTGFRVFCLLNATSLFISLAVVVVQIT 419

Query: 301 TSLTDHHQRVRYMGIASFFTTVAILAMVIAFAA 320
               D   + + + + +     A      AF A
Sbjct: 420 LVAWDTRAQKKVVSVVNKLMWAACACTFGAFLA 450

BLAST of MC08g_new0614 vs. NCBI nr
Match: XP_038877632.1 (ankyrin repeat-containing protein At5g02620-like [Benincasa hispida])

HSP 1 Score: 419 bits (1076), Expect = 5.68e-140
Identity = 219/336 (65.18%), Postives = 267/336 (79.46%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD LGLTPLHYA+ YGRT+AI LFL+ +SS++YILD NGESALHIAA  GH +AV+
Sbjct: 200 MIKEQDCLGLTPLHYASLYGRTKAINLFLQNESSSIYILDNNGESALHIAAFEGHKDAVE 259

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           +IL+ C DSCYLVD KGRTALHAAV+GDQRK ++ IL       ++NK D +GN+ LHLA
Sbjct: 260 EILNCCQDSCYLVDNKGRTALHAAVLGDQRKVVRLILGRAMQGRVMNKADGDGNMALHLA 319

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  E YD++EIL+   +V+LN KNKEFLTALD +NKHD+EGLRAA+ R LL+  TG MTM
Sbjct: 320 AFHEFYDIIEILATDVNVDLNAKNKEFLTALDFFNKHDQEGLRAAIIRNLLEGRTGPMTM 379

Query: 181 QQLAEKEIKKMNQEVKI-DIIPRSGSSGSSE----KRESLFTDKKTALEVNLLVATLVAT 240
           Q LAE++IKKMNQE+KI D    S   G ++    K  S  T+K+ ALEVNLLVATLVAT
Sbjct: 380 QHLAEEKIKKMNQEIKIMDDHDNSNEVGKTDGKMKKGSSYNTEKQKALEVNLLVATLVAT 439

Query: 241 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 300
           VTFAAGF+MPGG F++IG +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+TDH+QR
Sbjct: 440 VTFAAGFTMPGGYFENIGLSILSDKGGFRVFVIFNTIAFCCSVFAVLLHFHTSITDHYQR 499

Query: 301 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           VRYMGIA  FT++AI+AMVIAFA+GTHVV+  S  F
Sbjct: 500 VRYMGIAGSFTSIAIVAMVIAFASGTHVVIANSKAF 535

BLAST of MC08g_new0614 vs. NCBI nr
Match: KAA0063583.1 (protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYJ97910.1 protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 409 bits (1052), Expect = 2.25e-136
Identity = 214/336 (63.69%), Postives = 271/336 (80.65%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD+LGLTPLHYA  YGRT+AI LFL+ +SS++YI+D NGESALHIAA RG+ +AV+
Sbjct: 199 MIKEQDDLGLTPLHYATLYGRTKAINLFLQNESSSIYIVDNNGESALHIAAFRGYADAVE 258

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           +ILD C DSCYLVD KGRT LHAAV+GDQRK +K IL  P+   ++NK D +GN+ LH A
Sbjct: 259 EILDCCQDSCYLVDNKGRTPLHAAVLGDQRKVVKLILGRPKQGRVMNKADCDGNMALHHA 318

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  + YD++EIL+ +  V+LN+KN +FLTALD++NKHD EGLRAAV R +L  STG+MTM
Sbjct: 319 AFHKFYDIIEILATNEKVDLNVKNNKFLTALDIFNKHDLEGLRAAVIRNILVGSTGSMTM 378

Query: 181 QQLAEKEIKKMNQEVKI--DIIPRSGSSG--SSEKRESLF-TDKKTALEVNLLVATLVAT 240
           Q LA +EIKKMNQE++I  D    + ++G  S +K+ES + T+K+ ALEVNLLVATLVAT
Sbjct: 379 QHLAAEEIKKMNQEIEIEDDDDGDNDTNGIISIKKKESSYNTEKQKALEVNLLVATLVAT 438

Query: 241 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 300
           VTFAAGFSMPGG F+++G +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+ DH+QR
Sbjct: 439 VTFAAGFSMPGGYFENVGLSILSNKGGFKVFVIFNTIAFCCSVFAVLLHFHTSIADHYQR 498

Query: 301 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           VRYMGIA  FT++AI+AMVIAFA+GT VV+  +  F
Sbjct: 499 VRYMGIAVTFTSIAIVAMVIAFASGTFVVIANAKAF 534

BLAST of MC08g_new0614 vs. NCBI nr
Match: XP_008456265.1 (PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo])

HSP 1 Score: 408 bits (1048), Expect = 9.02e-136
Identity = 213/336 (63.39%), Postives = 272/336 (80.95%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD+LGLTPLHYA  YGRT+AI LFL+ +SS++YI+D NGESALHIAA RG+ +AV+
Sbjct: 199 MIKEQDDLGLTPLHYATLYGRTKAINLFLQNESSSIYIVDNNGESALHIAAFRGYADAVE 258

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           +IL+ C DSCYLVD KGRT LHAAV+GDQRK +K IL   +   ++NK D +GN+ LH A
Sbjct: 259 EILNCCQDSCYLVDNKGRTPLHAAVLGDQRKVVKLILGRAKQGRVMNKADCDGNMALHHA 318

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  + YD++EIL+ +  V+LN+KN +FLTALD++NKHD EGLRAAV R +L  STG+MTM
Sbjct: 319 AFHKFYDIIEILATNEKVDLNVKNNKFLTALDIFNKHDLEGLRAAVIRNILVGSTGSMTM 378

Query: 181 QQLAEKEIKKMNQEVKI--DIIPRSGSSG--SSEKRESLF-TDKKTALEVNLLVATLVAT 240
           Q LA +EIKKMNQE++I  D    + ++G  S +K+ES + T+K+ ALEVNLLVATLVAT
Sbjct: 379 QHLAAEEIKKMNQEIEIEDDDDGDNDTNGIISIKKKESSYNTEKQKALEVNLLVATLVAT 438

Query: 241 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 300
           VTFAAGFSMPGG F+++G +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+TDH+QR
Sbjct: 439 VTFAAGFSMPGGYFENVGLSILSNKGGFKVFVIFNTIAFCCSVFAVLLHFHTSITDHYQR 498

Query: 301 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           VRYMGIA  FT++AI+AMVIAFA+GT+VV+  +  F
Sbjct: 499 VRYMGIAVTFTSIAIVAMVIAFASGTYVVIANAQAF 534

BLAST of MC08g_new0614 vs. NCBI nr
Match: XP_011648740.1 (protein ACCELERATED CELL DEATH 6 [Cucumis sativus] >KGN60784.1 hypothetical protein Csa_019306 [Cucumis sativus])

HSP 1 Score: 407 bits (1046), Expect = 1.92e-135
Identity = 211/338 (62.43%), Postives = 266/338 (78.70%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD+LGLTPLHYA+ YGRTEAI LFL+ +SS++YI+D NGESALHIAA +GH +AV+
Sbjct: 199 MIKEQDDLGLTPLHYASLYGRTEAINLFLQNESSSIYIVDNNGESALHIAAFKGHKDAVE 258

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
            IL+ C DSCYLVD KGRT LHAAV+GDQRK +K IL   +   ++NK D +GN+ LH A
Sbjct: 259 AILNCCQDSCYLVDNKGRTPLHAAVLGDQRKVVKLILGRAKQGRVMNKADCDGNMALHHA 318

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  + YD++EIL+ S +V+ N+KNK  LTALD++NKHD EGLRAAV R +L  STG+MTM
Sbjct: 319 AFHKFYDIIEILATSENVDKNVKNKTSLTALDIFNKHDLEGLRAAVIRNILIGSTGSMTM 378

Query: 181 QQLAEKEIKKMNQEVKIDIIP-------RSGSSGSSEKRESLFTDKKTALEVNLLVATLV 240
           Q LA + IKKMNQE+KI+ +         +G +   +K  S  T+K+ ALEVNLLVATLV
Sbjct: 379 QHLATQAIKKMNQEIKIEELDDDDDDDDTNGINSIKQKESSYNTEKQKALEVNLLVATLV 438

Query: 241 ATVTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHH 300
           ATVTFAAGFSMPGG +++IG +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+TDH+
Sbjct: 439 ATVTFAAGFSMPGGYYENIGLSILSNKGGFKVFVIFNTIAFCCSVFAVLLHFHTSITDHY 498

Query: 301 QRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           QRVRYMGIA  FT++AI+AMVIAFA+GT+VV+  +  F
Sbjct: 499 QRVRYMGIAVTFTSIAIVAMVIAFASGTYVVMANAKAF 536

BLAST of MC08g_new0614 vs. NCBI nr
Match: XP_022143167.1 (ankyrin repeat-containing protein At5g02620-like [Momordica charantia])

HSP 1 Score: 327 bits (839), Expect = 3.64e-104
Identity = 177/337 (52.52%), Postives = 238/337 (70.62%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI   D+LG+TPLHYAA +G+ +AI+LF++  SSA+Y+L  NGESALHIAA +G+ NA+K
Sbjct: 213 MIREGDDLGMTPLHYAALHGKIDAIKLFJQYDSSAIYLLTNNGESALHIAAFQGYTNALK 272

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           ++++  PD   L+D KGRT LHAAV+G++  +++FIL  P L  LINK D +GN  LHLA
Sbjct: 273 ELINCRPDCYNLIDNKGRTPLHAAVLGNRLYSVEFILKTPTLERLINKQDCDGNTALHLA 332

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           AL + YD +E+++N       + N EFLT  DVY KHDEEGLRAAV  Y L +  G M M
Sbjct: 333 ALHKFYDAIEVITNHKIATWQIANNEFLTPFDVYKKHDEEGLRAAVACYNLKNCKGPMVM 392

Query: 181 QQLAEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDK------KTALEVNLLVATLVA 240
            Q A  +++KMN+E +      + ++ + + ++S   DK      +TALEVNLLVA LVA
Sbjct: 393 HQYANTQLEKMNKEAENATEEETSTNNNKDSKKS---DKSSDFGLETALEVNLLVAMLVA 452

Query: 241 TVTFAAGFSMPGG---NFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTD 300
           TVTFAAGF++PGG   + +  G AILT K +F VF + N+ AFC SVFAVLLQFHT++T 
Sbjct: 453 TVTFAAGFTVPGGFISSGEQXGLAILTKKPSFVVFTVSNSFAFCCSVFAVLLQFHTTITR 512

Query: 301 HHQRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRS 328
           H  RVRY G+A+  TT AILAMV+AF +G++VVLT+S
Sbjct: 513 HEARVRYKGVAASSTTAAILAMVLAFCSGSYVVLTKS 546

BLAST of MC08g_new0614 vs. ExPASy TrEMBL
Match: A0A5A7V660 (Protein ACCELERATED CELL DEATH 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001260 PE=4 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 1.09e-136
Identity = 214/336 (63.69%), Postives = 271/336 (80.65%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD+LGLTPLHYA  YGRT+AI LFL+ +SS++YI+D NGESALHIAA RG+ +AV+
Sbjct: 199 MIKEQDDLGLTPLHYATLYGRTKAINLFLQNESSSIYIVDNNGESALHIAAFRGYADAVE 258

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           +ILD C DSCYLVD KGRT LHAAV+GDQRK +K IL  P+   ++NK D +GN+ LH A
Sbjct: 259 EILDCCQDSCYLVDNKGRTPLHAAVLGDQRKVVKLILGRPKQGRVMNKADCDGNMALHHA 318

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  + YD++EIL+ +  V+LN+KN +FLTALD++NKHD EGLRAAV R +L  STG+MTM
Sbjct: 319 AFHKFYDIIEILATNEKVDLNVKNNKFLTALDIFNKHDLEGLRAAVIRNILVGSTGSMTM 378

Query: 181 QQLAEKEIKKMNQEVKI--DIIPRSGSSG--SSEKRESLF-TDKKTALEVNLLVATLVAT 240
           Q LA +EIKKMNQE++I  D    + ++G  S +K+ES + T+K+ ALEVNLLVATLVAT
Sbjct: 379 QHLAAEEIKKMNQEIEIEDDDDGDNDTNGIISIKKKESSYNTEKQKALEVNLLVATLVAT 438

Query: 241 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 300
           VTFAAGFSMPGG F+++G +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+ DH+QR
Sbjct: 439 VTFAAGFSMPGGYFENVGLSILSNKGGFKVFVIFNTIAFCCSVFAVLLHFHTSIADHYQR 498

Query: 301 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           VRYMGIA  FT++AI+AMVIAFA+GT VV+  +  F
Sbjct: 499 VRYMGIAVTFTSIAIVAMVIAFASGTFVVIANAKAF 534

BLAST of MC08g_new0614 vs. ExPASy TrEMBL
Match: A0A1S3C2T5 (protein ACCELERATED CELL DEATH 6-like OS=Cucumis melo OX=3656 GN=LOC103496257 PE=4 SV=1)

HSP 1 Score: 408 bits (1048), Expect = 4.37e-136
Identity = 213/336 (63.39%), Postives = 272/336 (80.95%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD+LGLTPLHYA  YGRT+AI LFL+ +SS++YI+D NGESALHIAA RG+ +AV+
Sbjct: 199 MIKEQDDLGLTPLHYATLYGRTKAINLFLQNESSSIYIVDNNGESALHIAAFRGYADAVE 258

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           +IL+ C DSCYLVD KGRT LHAAV+GDQRK +K IL   +   ++NK D +GN+ LH A
Sbjct: 259 EILNCCQDSCYLVDNKGRTPLHAAVLGDQRKVVKLILGRAKQGRVMNKADCDGNMALHHA 318

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  + YD++EIL+ +  V+LN+KN +FLTALD++NKHD EGLRAAV R +L  STG+MTM
Sbjct: 319 AFHKFYDIIEILATNEKVDLNVKNNKFLTALDIFNKHDLEGLRAAVIRNILVGSTGSMTM 378

Query: 181 QQLAEKEIKKMNQEVKI--DIIPRSGSSG--SSEKRESLF-TDKKTALEVNLLVATLVAT 240
           Q LA +EIKKMNQE++I  D    + ++G  S +K+ES + T+K+ ALEVNLLVATLVAT
Sbjct: 379 QHLAAEEIKKMNQEIEIEDDDDGDNDTNGIISIKKKESSYNTEKQKALEVNLLVATLVAT 438

Query: 241 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 300
           VTFAAGFSMPGG F+++G +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+TDH+QR
Sbjct: 439 VTFAAGFSMPGGYFENVGLSILSNKGGFKVFVIFNTIAFCCSVFAVLLHFHTSITDHYQR 498

Query: 301 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           VRYMGIA  FT++AI+AMVIAFA+GT+VV+  +  F
Sbjct: 499 VRYMGIAVTFTSIAIVAMVIAFASGTYVVIANAQAF 534

BLAST of MC08g_new0614 vs. ExPASy TrEMBL
Match: A0A0A0LIB0 (ANK_REP_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010200 PE=4 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 9.30e-136
Identity = 211/338 (62.43%), Postives = 266/338 (78.70%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI+ QD+LGLTPLHYA+ YGRTEAI LFL+ +SS++YI+D NGESALHIAA +GH +AV+
Sbjct: 199 MIKEQDDLGLTPLHYASLYGRTEAINLFLQNESSSIYIVDNNGESALHIAAFKGHKDAVE 258

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
            IL+ C DSCYLVD KGRT LHAAV+GDQRK +K IL   +   ++NK D +GN+ LH A
Sbjct: 259 AILNCCQDSCYLVDNKGRTPLHAAVLGDQRKVVKLILGRAKQGRVMNKADCDGNMALHHA 318

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           A  + YD++EIL+ S +V+ N+KNK  LTALD++NKHD EGLRAAV R +L  STG+MTM
Sbjct: 319 AFHKFYDIIEILATSENVDKNVKNKTSLTALDIFNKHDLEGLRAAVIRNILIGSTGSMTM 378

Query: 181 QQLAEKEIKKMNQEVKIDIIP-------RSGSSGSSEKRESLFTDKKTALEVNLLVATLV 240
           Q LA + IKKMNQE+KI+ +         +G +   +K  S  T+K+ ALEVNLLVATLV
Sbjct: 379 QHLATQAIKKMNQEIKIEELDDDDDDDDTNGINSIKQKESSYNTEKQKALEVNLLVATLV 438

Query: 241 ATVTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHH 300
           ATVTFAAGFSMPGG +++IG +IL+ K  F+VFVIFNT+AFC SVFAVLL FHTS+TDH+
Sbjct: 439 ATVTFAAGFSMPGGYYENIGLSILSNKGGFKVFVIFNTIAFCCSVFAVLLHFHTSITDHY 498

Query: 301 QRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 331
           QRVRYMGIA  FT++AI+AMVIAFA+GT+VV+  +  F
Sbjct: 499 QRVRYMGIAVTFTSIAIVAMVIAFASGTYVVMANAKAF 536

BLAST of MC08g_new0614 vs. ExPASy TrEMBL
Match: A0A6J1CQ03 (ankyrin repeat-containing protein At5g02620-like OS=Momordica charantia OX=3673 GN=LOC111013102 PE=4 SV=1)

HSP 1 Score: 328 bits (840), Expect = 1.25e-104
Identity = 178/337 (52.82%), Postives = 238/337 (70.62%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI   D+LG+TPLHYAA +G+ +AI+LFL+  SSA+Y+L  NGESALHIAA +G+ NA+K
Sbjct: 213 MIREGDDLGMTPLHYAALHGKIDAIKLFLQYDSSAIYLLTNNGESALHIAAFQGYTNALK 272

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           ++++  PD   L+D KGRT LHAAV+G++  +++FIL  P L  LINK D +GN  LHLA
Sbjct: 273 ELINCRPDCYNLIDNKGRTPLHAAVLGNRLYSVEFILKTPTLERLINKQDCDGNTALHLA 332

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
           AL + YD +E+++N       + N EFLT  DVY KHDEEGLRAAV  Y L +  G M M
Sbjct: 333 ALHKFYDAIEVITNHKIATWQIANNEFLTPFDVYKKHDEEGLRAAVACYNLKNCKGPMVM 392

Query: 181 QQLAEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDK------KTALEVNLLVATLVA 240
            Q A  +++KMN+E +      + ++ + + ++S   DK      +TALEVNLLVA LVA
Sbjct: 393 HQYANTQLEKMNKEAENATEEETSTNNNKDSKKS---DKSSDFGLETALEVNLLVAMLVA 452

Query: 241 TVTFAAGFSMPGG---NFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTD 300
           TVTFAAGF++PGG   + +  G AILT K +F VF + N+ AFC SVFAVLLQFHT++T 
Sbjct: 453 TVTFAAGFTVPGGFISSGEQXGLAILTKKPSFVVFTVSNSFAFCCSVFAVLLQFHTTITR 512

Query: 301 HHQRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRS 328
           H  RVRY G+A+  TT AILAMV+AF +G++VVLT+S
Sbjct: 513 HEARVRYKGVAASSTTAAILAMVLAFCSGSYVVLTKS 546

BLAST of MC08g_new0614 vs. ExPASy TrEMBL
Match: A0A6J1CNI9 (ankyrin-2-like OS=Momordica charantia OX=3673 GN=LOC111013103 PE=4 SV=1)

HSP 1 Score: 310 bits (793), Expect = 8.59e-98
Identity = 177/345 (51.30%), Postives = 237/345 (68.70%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           MI  +D LG+TPLHYAAFYGR EA++LFLE  SSA+ +L+ NG+SALHIAA  GHIN ++
Sbjct: 211 MIGKRDNLGMTPLHYAAFYGRIEAMKLFLEYDSSAILLLNKNGDSALHIAAFEGHINVLE 270

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIPLHLA 120
           +++   PD   L++ KGRT LH+AV+G Q  A++FIL  P L  L NK D +GN  LHLA
Sbjct: 271 ELIKCRPDCYNLINNKGRTPLHSAVLGRQVAAVEFILKTPTLERLTNKQDKDGNTALHLA 330

Query: 121 ALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGTMTM 180
            L + Y  + I+++S+ ++  + N  FLT  DV ++HDEE  RAAV+   LD   G MTM
Sbjct: 331 VLHKFYAAIHIITDSTKLSARIVNNRFLTPFDVSDEHDEEVSRAAVS--CLDLKMGLMTM 390

Query: 181 QQLAEKEIKKMNQEVKIDIIPRSGSSGS---SEKRESLFTDKKTALEVNLLVATLVATVT 240
            Q A+ +++KMN+E++ +      +S S   ++K +   ++   ALEV LLVA LVATVT
Sbjct: 391 HQSAKAQLEKMNKELRENGREEKKTSSSFILNKKSDDQNSELHAALEVILLVAMLVATVT 450

Query: 241 FAAGFSMPGG----NFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTD-- 300
           FAAGF++PGG    N +D G AILT K AF+VF++FNT+AFC SVFAVL Q H+SL D  
Sbjct: 451 FAAGFTVPGGFISSNGEDQGLAILTKKPAFKVFIVFNTLAFCCSVFAVLFQLHSSLIDYQ 510

Query: 301 --------HHQRVRYMGIASFFTTVAILAMVIAFAAGTHVVLTRS 328
                   H  RVRY+ I +FFTTVAILA+V+AF++GT VVLT S
Sbjct: 511 RRGSQCQDHQLRVRYIKITAFFTTVAILALVLAFSSGTFVVLTES 553

BLAST of MC08g_new0614 vs. TAIR 10
Match: AT4G03500.1 (Ankyrin repeat family protein )

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-33
Identity = 106/333 (31.83%), Postives = 169/333 (50.75%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           +I ++DE G T L + A  G  +      +K    +Y+ D +G    H+AA  GH+  ++
Sbjct: 273 LINLRDE-GRTSLSFGASIGYYQGFSYLFDKNRDKVYVSDDDGLFPTHMAAKYGHVQILE 332

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFIL---LEPRLAPLINKPDDNGNIPL 120
           +IL  CP++  L+D+ G+  LH A    + K IKFIL    +     LIN+ D NGN PL
Sbjct: 333 EILKHCPEAIELLDRDGQNILHLAAKYGKLKVIKFILSCCKDKNKKKLINEQDVNGNTPL 392

Query: 121 HLAALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTGT 180
           HLA +     VV + +    V+L  +N    TALDV  ++ +       + Y++      
Sbjct: 393 HLATINWHPKVVSMFTWDHRVDLKKRNYIGFTALDVAEENID-------SSYIVHQRLTW 452

Query: 181 MTMQQL-AEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDKKTALEVNLLVATLVATV 240
           M +    A K    + + ++    P  G           + D+   L   +LVATLVAT+
Sbjct: 453 MALINAGAPKSSTPITENLRSFKKPDGGK----------YKDRVNTL---MLVATLVATM 512

Query: 241 TFAAGFSMPGG---NFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHH 300
           TF AGF++PGG   +F  +G A+L  + AFQVF++ +T+A  SS+  ++      L D  
Sbjct: 513 TFTAGFTLPGGYNDSFPHLGMAVLAKRTAFQVFLVCDTLAMYSSIITIVALIWAQLGDLS 572

Query: 301 QRVRYMGIASFFTTVAILAMVIAFAAGTHVVLT 327
             ++   IA  F  +A+ +M IAF AGT+V ++
Sbjct: 573 IILKAFNIALPFLGLALTSMSIAFMAGTYVAVS 584

BLAST of MC08g_new0614 vs. TAIR 10
Match: AT1G03670.1 (ankyrin repeat family protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.5e-32
Identity = 99/333 (29.73%), Postives = 162/333 (48.65%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFL----EKKSSAMYILDANGESALHIAASRGHI 60
           +IE+++E G T L Y A  G  E I   L    +  SS  Y+ D +G + +H+AA  GH+
Sbjct: 241 LIELRNEEGRTCLSYGASMGCYEGIRYILAEFDKAASSLCYVADDDGFTPIHMAAKEGHV 300

Query: 61  NAVKQILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFILLEPRLAPLINKPDDNGNIP 120
             +K+ L  CPDS  L++ + +   H A +  + K +K++L       ++N+ D NGN P
Sbjct: 301 RIIKEFLKHCPDSRELLNNQCQNIFHVAAIAGKSKVVKYLLKLDEGKRMMNEQDINGNTP 360

Query: 121 LHLAALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSSTG 180
           LHLA       VV +L+ +  +NL   N E  TALD+                 +  +  
Sbjct: 361 LHLATKHRYPIVVNMLTWNDGINLRALNNEGFTALDIAE--------------TMKDNNA 420

Query: 181 TMTMQQLAEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDKKTALEVNLLVATLVATV 240
            +  ++L    +         ++IP + S  S +  E      K ++   ++ ATLVATV
Sbjct: 421 YVLYKRLIWMALVSAGAPHGPNLIPLTVSQSSKQSPERY----KDSVNTLMVTATLVATV 480

Query: 241 TFAAGFSMPGGNFQD---IGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHH 300
           TFAAG ++PGG       +G A L  K  F+VF++ N +A C+SV  V+      L D  
Sbjct: 481 TFAAGLTLPGGYMSSAPHLGMAALVNKLNFKVFLLLNNIAMCTSVVTVMALIWAQLGDAL 540

Query: 301 QRVRYMGIASFFTTVAILAMVIAFAAGTHVVLT 327
              +   +A      A+++M++A  AG  +V++
Sbjct: 541 LTKKAFRLALPLLLTAVVSMMMASVAGLTLVVS 555

BLAST of MC08g_new0614 vs. TAIR 10
Match: AT4G03460.1 (Ankyrin repeat family protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-31
Identity = 103/334 (30.84%), Postives = 164/334 (49.10%), Query Frame = 0

Query: 1   MIEVQDELGLTPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVK 60
           +I  +DE G T L + A  G  E     L+K   ++Y+ D +G   +H+A   G++  +K
Sbjct: 309 LINFRDE-GRTCLSFGASLGYYEGFCYLLDKALDSVYVSDDDGSFPIHMAVKYGYVKILK 368

Query: 61  QILDSCPDSCYLVDKKGRTALHAAVMGDQRKAIKFIL---LEPRLAPLINKPDDNGNIPL 120
            IL  CPD+  L+D++ +  LH A    + + +KFIL    +     LIN+ D NGN PL
Sbjct: 369 AILKRCPDALELLDRENQNVLHVAAKNGKIEVLKFILRCCKDKNKEKLINEEDANGNTPL 428

Query: 121 HLAALFELYDVVEILSNSSHVNLNLKNKEFLTALDVYNKHDEEGLRAAVTRYLLDSS--- 180
           HLA       VV +L+  + V+L   N + +TALD+  K+             +DSS   
Sbjct: 429 HLATKNWHPKVVSMLTWDNRVDLKTLNHDGVTALDIAEKN-------------MDSSYTF 488

Query: 181 TGTMTMQQLAEKEIKKMNQEVKIDIIPRSGSSGSSEKRESLFTDKKTALEVNLLVATLVA 240
              +T   L      +  + +    + ++   G  + R          +   LLVATLVA
Sbjct: 489 FERLTWMALISAGAPRGPKLILSTPVTQNSDGGKYKDR----------VNTLLLVATLVA 548

Query: 241 TVTFAAGFSMPG---GNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTD 300
           T+TF AGF++PG   G+  + G A L  K AFQVF++F+T+A   S+  ++      L D
Sbjct: 549 TMTFTAGFTLPGGYNGSVPNFGMATLAKKTAFQVFLVFDTLAMYCSIITIVALIWAQLGD 608

Query: 301 HHQRVRYMGIASFFTTVAILAMVIAFAAGTHVVL 326
               ++   +A     +A+ +M IAF AGT+  +
Sbjct: 609 LSLIMKAFNLALPLLGLALTSMSIAFMAGTYAAV 618

BLAST of MC08g_new0614 vs. TAIR 10
Match: AT3G12360.1 (Ankyrin repeat family protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-29
Identity = 97/332 (29.22%), Postives = 172/332 (51.81%), Query Frame = 0

Query: 11  TPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVKQILDSCPDSC 70
           TPL  AA  G TE +   L K  + + I  +N ++ALH+AA +GH+  +K +L   P   
Sbjct: 200 TPLVSAAMRGHTEVVNQLLSKAGNLLEISRSNNKNALHLAARQGHVEVIKALLSKDPQLA 259

Query: 71  YLVDKKGRTALHAAVMGDQRKAIKFIL-LEPRLAPLINKPDDNGNIPLHLAALFELYDVV 130
             +DKKG+TALH AV G   + +K +L  +P    ++ +PD + N  LH+A   +  ++V
Sbjct: 260 RRIDKKGQTALHMAVKGQSSEVVKLLLDADP---AIVMQPDKSCNTALHVATRKKRAEIV 319

Query: 131 EILSNSSHVNLNLKNKEFLTALDVYNKHDEEGL-RAAVTRYLLD--SSTGTMTMQQLAE- 190
           E+L +    N N   ++  TALD+      EGL  +  + Y+ +  + +G +   +L + 
Sbjct: 320 ELLLSLPDTNANTLTRDHKTALDI-----AEGLPLSEESSYIKECLARSGALRANELNQP 379

Query: 191 -KEIKKMNQEVKIDI---------IPRSGSSGSSEKRESLFTDKKTALEVNLLVATLVAT 250
             E++    ++K D+           ++  + S E R+        A     +VA L AT
Sbjct: 380 RDELRSTVTQIKNDVHIQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLFAT 439

Query: 251 VTFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQR 310
           V FAA F++PGG+  D G+A++  +A+F++F IFN +A  +S+  V++Q      +    
Sbjct: 440 VAFAAIFTVPGGDNND-GSAVVVGRASFKIFFIFNALALFTSLAVVVVQITLVRGETKAE 499

Query: 311 VRYMGIASFFTTVAILAMVIAFAAGTHVVLTR 328
            R + + +    +A +   +AF A +++V+ R
Sbjct: 500 KRVVEVINKLMWLASMCTSVAFLASSYIVVGR 522

BLAST of MC08g_new0614 vs. TAIR 10
Match: AT3G09550.1 (Ankyrin repeat family protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.3e-28
Identity = 99/335 (29.55%), Postives = 168/335 (50.15%), Query Frame = 0

Query: 11  TPLHYAAFYGRTEAIELFLEKKSSAMYILDANGESALHIAASRGHINAVKQILDSCPDSC 70
           TPL  AA  G +E +   L K SS + I  +NG++ALH+AA +GH++ V+ +LD  P   
Sbjct: 214 TPLVSAATRGHSEVVNELLAKDSSLLEISRSNGKNALHLAARQGHVDIVRTLLDKDPQLA 273

Query: 71  YLVDKKGRTALHAAVMGDQRKAIKFIL-LEPRLAPLINKPDDNGNIPLHLAALFELYDVV 130
              DKKG+T+LH AV G   + ++ +L  +P +  L   PD  GN  LH+A   +  ++V
Sbjct: 274 RRTDKKGQTSLHMAVKGVSSQVVRLLLRADPAIVML---PDKFGNTVLHIATRKKRAEIV 333

Query: 131 EILSNSSHVNLNLKNKEFLTALDVYN--KHDEEGLRAAVTRYLLDSSTGTMTMQQLAE-- 190
             L      N+N   ++  TA D+     H EE    A  + +L S  G +   +L +  
Sbjct: 334 NELLQLPDTNVNALTRDHKTAYDIAEGLTHSEE---TAEIKEIL-SRCGALKANELNQPR 393

Query: 191 KEIKKMNQEVKIDIIPRSGSSGSSEK---------RESLFTDKKTALEVNLLVATLVATV 250
            E++K   E+K D+  +   +  + K         R+        A     +VA L ATV
Sbjct: 394 DELRKTVTEIKKDVHTQLEQTRKTNKNVDGIAKELRKLHRAGINNATNSVTVVAVLFATV 453

Query: 251 TFAAGFSMPGGNFQDIGNAILTTKAAFQVFVIFNTVAFCSSVFAVLLQFHTSLTDHHQRV 310
            FAA F++PGG+  D G A++    +F++F IFN +A  +S+  V++Q      +     
Sbjct: 454 AFAAIFTVPGGD-DDHGVAVMVHATSFKIFFIFNAIALFTSLAVVVVQITLVRGETKTER 513

Query: 311 RYMGIASFFTTVAILAMVIAFAAGTHVVLTRSMKF 332
           R + + +    +A +   +AF + +++V+ R  ++
Sbjct: 514 RVVEVINKLMWLASVCTTVAFISSSYIVVGRRNRY 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C7A21.7e-2829.22Ankyrin repeat-containing protein ITN1 OS=Arabidopsis thaliana OX=3702 GN=ITN1 P... [more]
Q8LPS21.9e-2429.13Protein ACCELERATED CELL DEATH 6 OS=Arabidopsis thaliana OX=3702 GN=ACD6 PE=1 SV... [more]
A2CIR57.2e-2428.14Ankyrin repeat-containing protein NPR4 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q6AWW56.1e-2327.38Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q9ZU961.6e-1522.52Ankyrin repeat-containing protein At2g01680 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Match NameE-valueIdentityDescription
XP_038877632.15.68e-14065.18ankyrin repeat-containing protein At5g02620-like [Benincasa hispida][more]
KAA0063583.12.25e-13663.69protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYJ97910.1 pro... [more]
XP_008456265.19.02e-13663.39PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo][more]
XP_011648740.11.92e-13562.43protein ACCELERATED CELL DEATH 6 [Cucumis sativus] >KGN60784.1 hypothetical prot... [more]
XP_022143167.13.64e-10452.52ankyrin repeat-containing protein At5g02620-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5A7V6601.09e-13663.69Protein ACCELERATED CELL DEATH 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3C2T54.37e-13663.39protein ACCELERATED CELL DEATH 6-like OS=Cucumis melo OX=3656 GN=LOC103496257 PE... [more]
A0A0A0LIB09.30e-13662.43ANK_REP_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010... [more]
A0A6J1CQ031.25e-10452.82ankyrin repeat-containing protein At5g02620-like OS=Momordica charantia OX=3673 ... [more]
A0A6J1CNI98.59e-9851.30ankyrin-2-like OS=Momordica charantia OX=3673 GN=LOC111013103 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G03500.11.3e-3331.83Ankyrin repeat family protein [more]
AT1G03670.11.5e-3229.73ankyrin repeat family protein [more]
AT4G03460.11.3e-3130.84Ankyrin repeat family protein [more]
AT3G12360.11.2e-2929.22Ankyrin repeat family protein [more]
AT3G09550.11.3e-2829.55Ankyrin repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 76..108
e-value: 67.0
score: 12.4
coord: 112..142
e-value: 1.5
score: 17.8
coord: 146..180
e-value: 2600.0
score: 2.0
coord: 42..71
e-value: 0.0021
score: 27.3
coord: 8..38
e-value: 0.092
score: 21.9
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 8..31
score: 9.37738
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 42..64
score: 9.53764
IPR026961PGG domainPFAMPF13962PGGcoord: 220..323
e-value: 3.0E-27
score: 94.7
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 1..199
e-value: 1.0E-34
score: 121.7
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 2..154
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 8..65
e-value: 1.8E-10
score: 41.3
coord: 75..142
e-value: 2.6E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR24186:SF30ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKEcoord: 2..330
NoneNo IPR availablePANTHERPTHR24186PROTEIN PHOSPHATASE 1 REGULATORY SUBUNITcoord: 2..330
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 8..31
score: 9.356852
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 42..64
score: 9.516039

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g_new0614.1MC08g_new0614.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding