MC08g_new0582 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g_new0582 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.6e-86
Identity = 166/365 (45.48%), Postives = 222/365 (60.82%), Query Frame = 0

Query: 10  FSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIR---ENPNTTFSHLPYG 69
           F+ LL L+F LL      + L  C FD+IYQLGDS SDTGNLIR   + P  T +H PYG
Sbjct: 3   FARLLHLVFSLLVFAGITNGL-ICPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYG 62

Query: 70  ETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEALL 129
           ETF    TGRCS+G L++D+ A    LPL+NPYL ++    HGVNFAVAG+TAL    L 
Sbjct: 63  ETFPGTPTGRCSDGRLIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLA 122

Query: 130 RNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQG 189
              +     +S L  QL W  ++  SIC   ++C  KL++ALF++G IG ND NYA F  
Sbjct: 123 ARGVQVSDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPN 182

Query: 190 KTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDE 249
           +TI+E++  VP + + + NA  ++I  G +RVIVPG FPIGC+   L           D+
Sbjct: 183 RTIEEIRAYVPFITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDD 242

Query: 250 LHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEASL 309
           L CL  LN+L+ Y N   ++A+  L  E P  VI+Y DYYNA  ++ R+   LG +  SL
Sbjct: 243 LGCLSSLNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSL 302

Query: 310 QKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLIHDIFPK 369
            K CCGIGG YN++  + CG  GVPVCPNP ++I WDG H TQ  Y+ +A ++I  I   
Sbjct: 303 LKCCCGIGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKA 362

Query: 370 LHCIF 372
           L C +
Sbjct: 363 LKCSY 365

BLAST of MC08g_new0582 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 7.4e-78
Identity = 159/365 (43.56%), Postives = 204/365 (55.89%), Query Frame = 0

Query: 6   TTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTFSHLPY 65
           TT + SLL+ +LF+     S  H    C  ++IYQ GDSISDTGNLIR  P         
Sbjct: 3   TTKALSLLVFILFV-----SLVHSSDQCPINSIYQFGDSISDTGNLIRNGP--------- 62

Query: 66  GETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEAL 125
                                    A  P   P   R+   N  VNF V+GSTAL+    
Sbjct: 63  -------------------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSFF 122

Query: 126 LRNKILSPVTNSSLDHQLGWMHSHFESICL-NERDCIEKLRSALFLVGEIGGNDYNYALF 185
               +  P TN+ L  QL W   H  S C  +  DC   L+ +LF+VGEIGGNDYNY  F
Sbjct: 123 SERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFF 182

Query: 186 QGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAY 245
           QGK ++E++  +P VV  I  A  +VI  GA  V+VPGNFP+GC PIYLT F   DT  Y
Sbjct: 183 QGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 242

Query: 246 DELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEA 305
           D+  CL  LN  A  HN+Q+++AI  LR+E P   IVYGDYYNA  +++R      FD++
Sbjct: 243 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKS 302

Query: 306 SLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLIHDIF 365
              KSCCG GG YN++  +  G  GVPVC NPH+ ISWDG+HLTQK Y+FM+ +L + I 
Sbjct: 303 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 366 PKLHC 370
            ++ C
Sbjct: 363 SQIKC 320

BLAST of MC08g_new0582 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.3e-74
Identity = 150/358 (41.90%), Postives = 213/358 (59.50%), Query Frame = 0

Query: 9   SFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIR-ENPNT--TFSHLPY 68
           SF L+L    ++++S  +    R   + +I   GDSI+DTGN +   N N     + LPY
Sbjct: 13  SFLLILYYTTIVVASSES----RCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPY 72

Query: 69  GETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYL-NRDALTNHGVNFAVAGSTALSFEA 128
           GE+FFH  +GR S+G L++D+ A   GLP V PY  +++   N G+NFAV G+TAL    
Sbjct: 73  GESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPYFGSQNVSFNQGINFAVYGATALDRAF 132

Query: 129 LLRNKILSPVTNSSLDHQLGWMHSHFESICLNE-RDCIEKLRSALFLVGEIGGNDYNYAL 188
           L++  I S  TN SL  QL        ++C +  RDC E L  +L L+GEIGGNDYNY  
Sbjct: 133 LVKQGIKSDFTNISLSVQLNTFKQILPNLCASSTRDCREMLGDSLILMGEIGGNDYNYPF 192

Query: 189 FQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTA 248
           F+GK+I E+K++VP +++ I +A+  +I  G    +VPGNFPIGC   YLT FQT     
Sbjct: 193 FEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQTATVEH 252

Query: 249 YDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDE 308
                C+  LN    +HN+Q+K  ++ L++  PH  I+Y DYYN+L  + +     GF  
Sbjct: 253 DPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPAKYGFKN 312

Query: 309 ASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLIH 362
             L  +CCG+GG YNF + K CG  GV  C NP E+++WDG HLT+ TY+ MA  L++
Sbjct: 313 RPL-AACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQGLLN 365

BLAST of MC08g_new0582 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 281.2 bits (718), Expect = 1.7e-74
Identity = 144/331 (43.50%), Postives = 208/331 (62.84%), Query Frame = 0

Query: 35  FDAIYQLGDSISDTGNLI-RENPN--TTFSHLPYGETFFHGATGRCSNGLLMLDYFALDA 94
           F +I   GDSI+DTGNL+   +PN     +  PYGETFFH  TGR S+G L++D+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 95  GLPLVNPYLN-RDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHF 154
           G PLV P+   ++A    GVNFAVAG+TAL    L    I S +TN SL  QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 155 ESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKV 214
            ++C +  DC + + +AL L+GEIGGNDYN+ALFQ K ++EV+++VP V+ TI +A+ ++
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDEL-HCLKGLNSLANYHNDQIKQAIE 274
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK LN  + Y+N Q+++ + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 275 VLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEASLQKSCCGIGGNYNFELMKICGLPG 334
            LR+  PH  I+Y DYYNALL + +     GF    L  +CCG+GG+YNF   + CG  G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 335 VPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 361
           V  C +P +++++DGIH+T+  Y+ ++  L+
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of MC08g_new0582 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.9e-74
Identity = 141/327 (43.12%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 35  FDAIYQLGDSISDTGNLIRENPNTTF---SHLPYGETFFHGATGRCSNGLLMLDYFALDA 94
           F +I   GDSI+DTGNL+  +  T     + LPYGETFFH  TGR SNG L++D+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 95  GLPLVNP-YLNRDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHF 154
           G PLV P Y +++A    GVNFAV G+TAL    L    I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 155 ESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKV 214
            ++C++  DC + + ++L L+GEIGGNDYNYA F GK I+E+K++VP V++TI +A+ ++
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDEL-HCLKGLNSLANYHNDQIKQAIE 274
           I  G    +VPG FP+GC   YL+ +QT++   YD L  CLK LN  + YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 275 VLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEASLQKSCCGIGGNYNFELMKICGLPG 334
            L++  PH  I+Y DYYN LL + +     GF    L  +CC +GG +NF L +  G   
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNFTLGRKRGTQV 331

Query: 335 VPVCPNPHEHISWDGIHLTQKTYKFMA 357
              C +P +++SWDG+H+T+  Y+ MA
Sbjct: 332 PECCDDPSKYVSWDGVHMTEAAYRLMA 357

BLAST of MC08g_new0582 vs. NCBI nr
Match: XP_022156428.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 761 bits (1966), Expect = 7.34e-278
Identity = 365/371 (98.38%), Postives = 368/371 (99.19%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           MAISFTTSSFSLLLPLLFLLLSS STAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF
Sbjct: 1   MAISFTTSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN
Sbjct: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           TTAYDELHCLKGLNSLA+YHNDQIKQAIEVL+RENPHTVIVYGDYYNALLWI+RHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FDEASLQKSCCGIGGNYNF LMKICGLPGVPVCPNPHEHISWDGIHLTQK YKFMAYWLI
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC08g_new0582 vs. NCBI nr
Match: XP_022158415.1 (GDSL esterase/lipase At5g03980-like [Momordica charantia])

HSP 1 Score: 724 bits (1870), Expect = 3.16e-263
Identity = 347/371 (93.53%), Postives = 354/371 (95.42%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           MA S TTS FSLLLPLLFLLLSSPSTAHPL ACMF AIYQLGDSISDTGNLIREN NTTF
Sbjct: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S LPYGETFFHGATGRCSNGLLMLDYFAL A LPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           S EALLRNKI SPVTNSSLDHQLGWMHSHF SICLNERDCI+KLRSALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           T AYDELHCLKG+NSLA+YHNDQIK AIEVLRRENPHTVIVYGDYYNALLWI+RHAFVLG
Sbjct: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FDEASLQKSCCGIGGNYNF LMKICGLPGVP+CPNPHEHISWDGIHLT+K YKFMAYW+I
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC08g_new0582 vs. NCBI nr
Match: XP_022156343.1 (GDSL esterase/lipase At5g03980-like [Momordica charantia])

HSP 1 Score: 711 bits (1835), Expect = 6.84e-258
Identity = 341/370 (92.16%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           M  S T+SS SLLLPLLFLLLS  STAH LR CMFDAIYQLGDSISDTGNLIRENPNTTF
Sbjct: 1   MPTSTTSSSLSLLLPLLFLLLSPXSTAHLLRTCMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S LPYGETFFHGATGRCSNGLLMLDYFALD GLPLVNPYLN++AL N GVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALDXGLPLVNPYLNKBALKNRGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           S E L RNKI SPVTNSSLD QL WMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN
Sbjct: 121 SSETLFRNKIFSPVTNSSLDRQLXWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           TTAYDELHCLKGLNSLA+YHNDQIKQAIEVL+RENPHTVIVYGDYYNALLWI+RHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FDEASLQKSCCGIGGNYNF L+KICGLP VP+CPNPHEHISWDGIHLTQK YKFMAYWLI
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLIKICGLPDVPICPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCI 370
           HDIFPKLHCI
Sbjct: 361 HDIFPKLHCI 370

BLAST of MC08g_new0582 vs. NCBI nr
Match: XP_022150751.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 689 bits (1779), Expect = 6.78e-250
Identity = 321/338 (94.97%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 34  MFDAIYQLGDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLLMLDYFALDAGL 93
           MFDAIYQ GDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLL LDYFALDAGL
Sbjct: 1   MFDAIYQXGDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLLXLDYFALDAGL 60

Query: 94  PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHFESI 153
           PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKI SPVTNSSLDHQLGWMHSHF SI
Sbjct: 61  PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKIFSPVTNSSLDHQLGWMHSHFNSI 120

Query: 154 CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKVISY 213
           CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKT+QEVKDMVPD+VQTIKNAVEKVISY
Sbjct: 121 CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTMQEVKDMVPDIVQTIKNAVEKVISY 180

Query: 214 GATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLANYHNDQIKQAIEVLRR 273
           GATRVI+PGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLA+YHND+IKQ IEVLR 
Sbjct: 181 GATRVIIPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYHNDRIKQVIEVLRM 240

Query: 274 ENPHTVIVYGDYYNALLWIIRHAFVLGFDEASLQKSCCGIGGNYNFELMKICGLPGVPVC 333
           ENPHTVIVYGDYYNALLWI+RHAFVLGFDEASLQKSCCGIGGNYNF+LMKICGLPGVPVC
Sbjct: 241 ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFDLMKICGLPGVPVC 300

Query: 334 PNPHEHISWDGIHLTQKTYKFMAYWLIHDIFPKLHCIF 371
           PNPHEHISWDGIHLT+K YKFMAYW+I+DIFPKLHCIF
Sbjct: 301 PNPHEHISWDGIHLTEKAYKFMAYWIINDIFPKLHCIF 338

BLAST of MC08g_new0582 vs. NCBI nr
Match: XP_022150750.1 (GDSL esterase/lipase At5g03980-like [Momordica charantia])

HSP 1 Score: 648 bits (1671), Expect = 4.25e-232
Identity = 315/360 (87.50%), Postives = 330/360 (91.67%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           MA S TTSSFSLLLPLLFLLLSSPS AHPLRACMFDAIYQLGDSISDTGNLIRENPNT F
Sbjct: 1   MATSSTTSSFSLLLPLLFLLLSSPSAAHPLRACMFDAIYQLGDSISDTGNLIRENPNTPF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SH  YGE+ FHGATGRCSNGLLMLDYFAL AGLP VNPYLN+D LT+HGVNFAVAGSTAL
Sbjct: 61  SHPLYGESLFHGATGRCSNGLLMLDYFALAAGLPSVNPYLNKDGLTDHGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           S EALLRNKILSPVT SSLDHQL WM SHF SI LNERDC EKL SALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKILSPVTXSSLDHQLDWMFSHFNSIYLNERDCNEKLGSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTI E KDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLT FQTND
Sbjct: 181 YALFQGKTISEAKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTEFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           TTAYDELHCLK LNSLA+YHNDQIKQAI+VLR+ENPHT+IVYGDYYNA LWI+R A +LG
Sbjct: 241 TTAYDELHCLKSLNSLASYHNDQIKQAIDVLRKENPHTIIVYGDYYNAFLWILRDASLLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FD ASLQKSCCGIGG+YNF L+++C LPGVP+CPNP E ISWDGIHLT+KTYKFMAYWLI
Sbjct: 301 FDGASLQKSCCGIGGDYNFNLVEMCRLPGVPICPNPDERISWDGIHLTEKTYKFMAYWLI 360

BLAST of MC08g_new0582 vs. ExPASy TrEMBL
Match: A0A6J1DQL3 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 SV=1)

HSP 1 Score: 761 bits (1966), Expect = 3.55e-278
Identity = 365/371 (98.38%), Postives = 368/371 (99.19%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           MAISFTTSSFSLLLPLLFLLLSS STAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF
Sbjct: 1   MAISFTTSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN
Sbjct: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           TTAYDELHCLKGLNSLA+YHNDQIKQAIEVL+RENPHTVIVYGDYYNALLWI+RHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FDEASLQKSCCGIGGNYNF LMKICGLPGVPVCPNPHEHISWDGIHLTQK YKFMAYWLI
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC08g_new0582 vs. ExPASy TrEMBL
Match: A0A6J1DVS3 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111024903 PE=3 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 1.53e-263
Identity = 347/371 (93.53%), Postives = 354/371 (95.42%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           MA S TTS FSLLLPLLFLLLSSPSTAHPL ACMF AIYQLGDSISDTGNLIREN NTTF
Sbjct: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S LPYGETFFHGATGRCSNGLLMLDYFAL A LPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           S EALLRNKI SPVTNSSLDHQLGWMHSHF SICLNERDCI+KLRSALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           T AYDELHCLKG+NSLA+YHNDQIK AIEVLRRENPHTVIVYGDYYNALLWI+RHAFVLG
Sbjct: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FDEASLQKSCCGIGGNYNF LMKICGLPGVP+CPNPHEHISWDGIHLT+K YKFMAYW+I
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC08g_new0582 vs. ExPASy TrEMBL
Match: A0A6J1DQD3 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111023260 PE=3 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 1.64e-258
Identity = 342/370 (92.43%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           M  S T+SS SLLLPLLFLLLS  STAH LR CMFDAIYQLGDSISDTGNLIRENPNTTF
Sbjct: 1   MPTSTTSSSLSLLLPLLFLLLSPXSTAHLLRTCMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S LPYGETFFHGATGRCSNGLLMLDYFALD GLPLVNPYLN+DAL N GVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALDXGLPLVNPYLNKDALKNRGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           S E L RNKI SPVTNSSLD QL WMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN
Sbjct: 121 SSETLFRNKIFSPVTNSSLDRQLXWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           TTAYDELHCLKGLNSLA+YHNDQIKQAIEVL+RENPHTVIVYGDYYNALLWI+RHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FDEASLQKSCCGIGGNYNF L+KICGLP VP+CPNPHEHISWDGIHLTQK YKFMAYWLI
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLIKICGLPDVPICPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCI 370
           HDIFPKLHCI
Sbjct: 361 HDIFPKLHCI 370

BLAST of MC08g_new0582 vs. ExPASy TrEMBL
Match: A0A6J1DCG0 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111018810 PE=3 SV=1)

HSP 1 Score: 689 bits (1779), Expect = 3.28e-250
Identity = 321/338 (94.97%), Postives = 331/338 (97.93%), Query Frame = 0

Query: 34  MFDAIYQLGDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLLMLDYFALDAGL 93
           MFDAIYQ GDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLL LDYFALDAGL
Sbjct: 1   MFDAIYQXGDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLLXLDYFALDAGL 60

Query: 94  PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHFESI 153
           PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKI SPVTNSSLDHQLGWMHSHF SI
Sbjct: 61  PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKIFSPVTNSSLDHQLGWMHSHFNSI 120

Query: 154 CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKVISY 213
           CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKT+QEVKDMVPD+VQTIKNAVEKVISY
Sbjct: 121 CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTMQEVKDMVPDIVQTIKNAVEKVISY 180

Query: 214 GATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLANYHNDQIKQAIEVLRR 273
           GATRVI+PGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLA+YHND+IKQ IEVLR 
Sbjct: 181 GATRVIIPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYHNDRIKQVIEVLRM 240

Query: 274 ENPHTVIVYGDYYNALLWIIRHAFVLGFDEASLQKSCCGIGGNYNFELMKICGLPGVPVC 333
           ENPHTVIVYGDYYNALLWI+RHAFVLGFDEASLQKSCCGIGGNYNF+LMKICGLPGVPVC
Sbjct: 241 ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFDLMKICGLPGVPVC 300

Query: 334 PNPHEHISWDGIHLTQKTYKFMAYWLIHDIFPKLHCIF 371
           PNPHEHISWDGIHLT+K YKFMAYW+I+DIFPKLHCIF
Sbjct: 301 PNPHEHISWDGIHLTEKAYKFMAYWIINDIFPKLHCIF 338

BLAST of MC08g_new0582 vs. ExPASy TrEMBL
Match: A0A6J1D9D2 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111018809 PE=3 SV=1)

HSP 1 Score: 648 bits (1671), Expect = 2.06e-232
Identity = 315/360 (87.50%), Postives = 330/360 (91.67%), Query Frame = 0

Query: 1   MAISFTTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60
           MA S TTSSFSLLLPLLFLLLSSPS AHPLRACMFDAIYQLGDSISDTGNLIRENPNT F
Sbjct: 1   MATSSTTSSFSLLLPLLFLLLSSPSAAHPLRACMFDAIYQLGDSISDTGNLIRENPNTPF 60

Query: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SH  YGE+ FHGATGRCSNGLLMLDYFAL AGLP VNPYLN+D LT+HGVNFAVAGSTAL
Sbjct: 61  SHPLYGESLFHGATGRCSNGLLMLDYFALAAGLPSVNPYLNKDGLTDHGVNFAVAGSTAL 120

Query: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180
           S EALLRNKILSPVT SSLDHQL WM SHF SI LNERDC EKL SALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKILSPVTXSSLDHQLDWMFSHFNSIYLNERDCNEKLGSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTI E KDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLT FQTND
Sbjct: 181 YALFQGKTISEAKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTEFQTND 240

Query: 241 TTAYDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLG 300
           TTAYDELHCLK LNSLA+YHNDQIKQAI+VLR+ENPHT+IVYGDYYNA LWI+R A +LG
Sbjct: 241 TTAYDELHCLKSLNSLASYHNDQIKQAIDVLRKENPHTIIVYGDYYNAFLWILRDASLLG 300

Query: 301 FDEASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 360
           FD ASLQKSCCGIGG+YNF L+++C LPGVP+CPNP E ISWDGIHLT+KTYKFMAYWLI
Sbjct: 301 FDGASLQKSCCGIGGDYNFNLVEMCRLPGVPICPNPDERISWDGIHLTEKTYKFMAYWLI 360

BLAST of MC08g_new0582 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 5.3e-79
Identity = 159/365 (43.56%), Postives = 204/365 (55.89%), Query Frame = 0

Query: 6   TTSSFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTFSHLPY 65
           TT + SLL+ +LF+     S  H    C  ++IYQ GDSISDTGNLIR  P         
Sbjct: 3   TTKALSLLVFILFV-----SLVHSSDQCPINSIYQFGDSISDTGNLIRNGP--------- 62

Query: 66  GETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEAL 125
                                    A  P   P   R+   N  VNF V+GSTAL+    
Sbjct: 63  -------------------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSFF 122

Query: 126 LRNKILSPVTNSSLDHQLGWMHSHFESICL-NERDCIEKLRSALFLVGEIGGNDYNYALF 185
               +  P TN+ L  QL W   H  S C  +  DC   L+ +LF+VGEIGGNDYNY  F
Sbjct: 123 SERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGFF 182

Query: 186 QGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAY 245
           QGK ++E++  +P VV  I  A  +VI  GA  V+VPGNFP+GC PIYLT F   DT  Y
Sbjct: 183 QGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 242

Query: 246 DELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEA 305
           D+  CL  LN  A  HN+Q+++AI  LR+E P   IVYGDYYNA  +++R      FD++
Sbjct: 243 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKS 302

Query: 306 SLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLIHDIF 365
              KSCCG GG YN++  +  G  GVPVC NPH+ ISWDG+HLTQK Y+FM+ +L + I 
Sbjct: 303 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 366 PKLHC 370
            ++ C
Sbjct: 363 SQIKC 320

BLAST of MC08g_new0582 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 9.3e-76
Identity = 150/358 (41.90%), Postives = 213/358 (59.50%), Query Frame = 0

Query: 9   SFSLLLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLIR-ENPNT--TFSHLPY 68
           SF L+L    ++++S  +    R   + +I   GDSI+DTGN +   N N     + LPY
Sbjct: 13  SFLLILYYTTIVVASSES----RCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPY 72

Query: 69  GETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYL-NRDALTNHGVNFAVAGSTALSFEA 128
           GE+FFH  +GR S+G L++D+ A   GLP V PY  +++   N G+NFAV G+TAL    
Sbjct: 73  GESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPYFGSQNVSFNQGINFAVYGATALDRAF 132

Query: 129 LLRNKILSPVTNSSLDHQLGWMHSHFESICLNE-RDCIEKLRSALFLVGEIGGNDYNYAL 188
           L++  I S  TN SL  QL        ++C +  RDC E L  +L L+GEIGGNDYNY  
Sbjct: 133 LVKQGIKSDFTNISLSVQLNTFKQILPNLCASSTRDCREMLGDSLILMGEIGGNDYNYPF 192

Query: 189 FQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTA 248
           F+GK+I E+K++VP +++ I +A+  +I  G    +VPGNFPIGC   YLT FQT     
Sbjct: 193 FEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQTATVEH 252

Query: 249 YDELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDE 308
                C+  LN    +HN+Q+K  ++ L++  PH  I+Y DYYN+L  + +     GF  
Sbjct: 253 DPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPAKYGFKN 312

Query: 309 ASLQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMAYWLIH 362
             L  +CCG+GG YNF + K CG  GV  C NP E+++WDG HLT+ TY+ MA  L++
Sbjct: 313 RPL-AACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQGLLN 365

BLAST of MC08g_new0582 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 281.2 bits (718), Expect = 1.2e-75
Identity = 144/331 (43.50%), Postives = 208/331 (62.84%), Query Frame = 0

Query: 35  FDAIYQLGDSISDTGNLI-RENPN--TTFSHLPYGETFFHGATGRCSNGLLMLDYFALDA 94
           F +I   GDSI+DTGNL+   +PN     +  PYGETFFH  TGR S+G L++D+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 95  GLPLVNPYLN-RDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHF 154
           G PLV P+   ++A    GVNFAVAG+TAL    L    I S +TN SL  QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 155 ESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKV 214
            ++C +  DC + + +AL L+GEIGGNDYN+ALFQ K ++EV+++VP V+ TI +A+ ++
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDEL-HCLKGLNSLANYHNDQIKQAIE 274
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK LN  + Y+N Q+++ + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 275 VLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEASLQKSCCGIGGNYNFELMKICGLPG 334
            LR+  PH  I+Y DYYNALL + +     GF    L  +CCG+GG+YNF   + CG  G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 335 VPVCPNPHEHISWDGIHLTQKTYKFMAYWLI 361
           V  C +P +++++DGIH+T+  Y+ ++  L+
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of MC08g_new0582 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 2.1e-75
Identity = 141/327 (43.12%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 35  FDAIYQLGDSISDTGNLIRENPNTTF---SHLPYGETFFHGATGRCSNGLLMLDYFALDA 94
           F +I   GDSI+DTGNL+  +  T     + LPYGETFFH  TGR SNG L++D+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 95  GLPLVNP-YLNRDALTNHGVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHF 154
           G PLV P Y +++A    GVNFAV G+TAL    L    I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 155 ESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKV 214
            ++C++  DC + + ++L L+GEIGGNDYNYA F GK I+E+K++VP V++TI +A+ ++
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYDEL-HCLKGLNSLANYHNDQIKQAIE 274
           I  G    +VPG FP+GC   YL+ +QT++   YD L  CLK LN  + YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 275 VLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEASLQKSCCGIGGNYNFELMKICGLPG 334
            L++  PH  I+Y DYYN LL + +     GF    L  +CC +GG +NF L +  G   
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNFTLGRKRGTQV 331

Query: 335 VPVCPNPHEHISWDGIHLTQKTYKFMA 357
              C +P +++SWDG+H+T+  Y+ MA
Sbjct: 332 PECCDDPSKYVSWDGVHMTEAAYRLMA 357

BLAST of MC08g_new0582 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.3e-74
Identity = 145/351 (41.31%), Postives = 210/351 (59.83%), Query Frame = 0

Query: 13  LLPLLFLLLSSPSTAHPLRACMFDAIYQLGDSISDTGNLI-----RENPNTTFSHLPYGE 72
           L   LF+ + S  T  P     F +I   GDSI+DTGNL+      + P T F   PYGE
Sbjct: 11  LFSTLFVTIVSSETPCP----NFKSIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGE 70

Query: 73  TFFHGATGRCSNGLLMLDYFALDAGLPLVNPYL-NRDALTNHGVNFAVAGSTALSFEALL 132
           TFFH  TGR  +G +++D+ A   GLP V PY  +++   + GVNFAVAG+TAL   + L
Sbjct: 71  TFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALK-SSFL 130

Query: 133 RNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQG 192
           + + + P TN SL  QL        ++C +  DC + + +AL L+GEIGGNDYN+  F  
Sbjct: 131 KKRGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNR 190

Query: 193 KTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYD- 252
           K ++EV+++VP V+ +I + + ++I  G    +VPG FPIGC  +YLT ++T++   YD 
Sbjct: 191 KPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDP 250

Query: 253 ELHCLKGLNSLANYHNDQIKQAIEVLRRENPHTVIVYGDYYNALLWIIRHAFVLGFDEAS 312
              CLK LN    YH++++K  +  LR+  PH  I+Y DYYN+LL I +     GF E  
Sbjct: 251 STGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERP 310

Query: 313 LQKSCCGIGGNYNFELMKICGLPGVPVCPNPHEHISWDGIHLTQKTYKFMA 357
              +CCGIGG YNF   + CG  GV  C +P +++ WDG+H+T+  YK++A
Sbjct: 311 F-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIA 353