MC08g_new0302 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0302
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	exopolygalacturonase-like
Location	MC08: 16057642 .. 16059467 (-)
RNA-Seq Expression	MC08g_new0302
Synteny	MC08g_new0302

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACATTCTAAAAAATACTCGACCAAAAAAAAATAGAAAAAAGCAAAAAAGAAAAAAAAAAGTAGAAGAAGAACAAGATGGGAATGGAGTTGAATTTGGTGACTTTATCCCTCATATTATTATTTGTATCAATCGTGGAAGCTCAGTCTAGCGATGGTATTTTTGATGTGACAAAGTTTGGTGCTAGACCTAATGCTGATATATCTAAGGTATAAAGAAAGTGTTTAATATTTGTTTATGAGATGGGAAACAAAAACTATGATAATTGTTAATTATGTTTTAATTTATGTATAGGCTCTGATGAGTGCATGGAAGGAAGCTTGTGCATCACCGATTCAAAGTAAAGTAATTATTCCGAAGGTGGCTTATAAGTTAGGTACAATTGATTTGGCAGGCCCTTGCAAAAGCCCTATCGAAGTTCAACTCCAAGGAACAATACAGGCACCAATAGATCTTATCGGAGAAGGTTGGGTTTAGTTTAAATATATCGACCACTTTACATTATCAGGTGGTGGAACTTTTGATGGCCAAGGAAAAGCAGCTTGGGAAAAAAATTATTGTGACAAAAATCCCAAATGTGATAGACTCCCATGGTACGTGCACTATCATCTAATCAATTTCTTTTTGGTGATTTCCATATATCATAGTTTATATGTTTATTAATCAATCTTATGGTTAGGATTTTAAAAATCAAACTGTCATCCCATATTTTTTTCTCTTAATTAAATATACGTATGCATGAGAATATTTGTTTACTATAAATGTGCAGAGTTTGAGGTTTAGCTTCATCACAAATTCGATCGTGAGTGACATAACTTCTAAAGATAGCAAAAATTTTCATGTACAAATTTTGGGTTGTAACAACCTTACCTTCCAACACGTGAATGTCAATGCGCCGGGAGATAGTATCAATACAGATGGAATTCATATTGGTCGTTCTACTGGGATTAATATCCTAAGTACAAACATCGGTACCGGAGATGATTGTATCTCACTTGGAGATGGTAGTAAGCAAGTAACAATTACTAATATGACATGCGGTCCTGGACATGGTATAAGCGTAGGAAGTCTTGGAAGGTACACTGCCGAAGAACCAGTTGAAGGAATCAGAGCTACAAATTGTACAATAATGAATACATCCAATGGTGTTAGAATCAAAACTTGGCCAGCTTCTCCTACTGCTACTGGAACCGCTTCTGATATGCATTTCGAAGATATGATAATGGTTAATGTTAGCAATCCGATTCTTATAGACCAAGAATATTGTCCATACAACCAATGTAACAAAAAGGTATTGGACTTTTGGTACTTTTAAATTTATTAATTAATTAATTTATTATTATTGTTATTATTATTTTTATTTTTTTTTGCATTTAGAAATGTTTAAATGAAACTTGAAGAAATAATGTTATATATATATTGTTTTCCAGAGTCCATCAAAAGTGAAGATTAGCAAGGTTAGCTTCAAGAATGTCAGAGGTTCTTCAGCCAATGCACTTGCACTCAAACTTGTCTGTAGTGCCGAAGTTCCATGTGAAGATGTAGAAGTTGTTGAAATTGATCTCACTTACAATGGAAACGAAGGCCCTATTTCATCTCAGTGTAAAAACGTGAAGCCCATCATTTCTGGAAAACAAAACCCTCGTGCTTGTTCTTCCCCTGCTCCACCAGCATAAGTAATTGCGTAGATGATCATTTGATGTAGAAAATGGTGGAAGATGATCTATCTATTCTAAAGCTTTGTAATTATAGAACTTTGTAGCACTCAAGTTTTCATCGGAAAAACTTGTTAAAAAGGATTTTTCTTCTTCATATTTACAAATAAAGA

mRNA sequence

ATGAGTTTGAGGTTTAGCTTCATCACAAATTCGATCGTGAGTGACATAACTTCTAAAGATAGCAAAAATTTTCATGTACAAATTTTGGGTTGTAACAACCTTACCTTCCAACACGTGAATGTCAATGCGCCGGGAGATAGTATCAATACAGATGGAATTCATATTGGTCGTTCTACTGGGATTAATATCCTAAGTACAAACATCGGTACCGGAGATGATTGTATCTCACTTGGAGATGGTAGTAAGCAAGTAACAATTACTAATATGACATGCGGTCCTGGACATGGTATAAGCGTAGGAAGTCTTGGAAGGTACACTGCCGAAGAACCAGTTGAAGGAATCAGAGCTACAAATTGTACAATAATGAATACATCCAATGGTGTTAGAATCAAAACTTGGCCAGCTTCTCCTACTGCTACTGGAACCGCTTCTGATATGCATTTCGAAGATATGATAATGGTTAATGTTAGCAATCCGATTCTTATAGACCAAGAATATTGTCCATACAACCAATGTAACAAAAAGAGTCCATCAAAAGTGAAGATTAGCAAGGTTAGCTTCAAGAATGTCAGAGGTTCTTCAGCCAATGCACTTGCACTCAAACTTGTCTGTAGTGCCGAAGTTCCATGTGAAGATGTAGAAGTTGTTGAAATTGATCTCACTTACAATGGAAACGAAGGCCCTATTTCATCTCAGTGTAAAAACGTGAAGCCCATCATTTCTGGAAAACAAAACCCTCGTGCTTGTTCTTCCCCTGCTCCACCAGCATAA

Coding sequence (CDS)

ATGAGTTTGAGGTTTAGCTTCATCACAAATTCGATCGTGAGTGACATAACTTCTAAAGATAGCAAAAATTTTCATGTACAAATTTTGGGTTGTAACAACCTTACCTTCCAACACGTGAATGTCAATGCGCCGGGAGATAGTATCAATACAGATGGAATTCATATTGGTCGTTCTACTGGGATTAATATCCTAAGTACAAACATCGGTACCGGAGATGATTGTATCTCACTTGGAGATGGTAGTAAGCAAGTAACAATTACTAATATGACATGCGGTCCTGGACATGGTATAAGCGTAGGAAGTCTTGGAAGGTACACTGCCGAAGAACCAGTTGAAGGAATCAGAGCTACAAATTGTACAATAATGAATACATCCAATGGTGTTAGAATCAAAACTTGGCCAGCTTCTCCTACTGCTACTGGAACCGCTTCTGATATGCATTTCGAAGATATGATAATGGTTAATGTTAGCAATCCGATTCTTATAGACCAAGAATATTGTCCATACAACCAATGTAACAAAAAGAGTCCATCAAAAGTGAAGATTAGCAAGGTTAGCTTCAAGAATGTCAGAGGTTCTTCAGCCAATGCACTTGCACTCAAACTTGTCTGTAGTGCCGAAGTTCCATGTGAAGATGTAGAAGTTGTTGAAATTGATCTCACTTACAATGGAAACGAAGGCCCTATTTCATCTCAGTGTAAAAACGTGAAGCCCATCATTTCTGGAAAACAAAACCCTCGTGCTTGTTCTTCCCCTGCTCCACCAGCATAA

Protein sequence

MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTGINILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCTIMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKVKISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPIISGKQNPRACSSPAPPA

Homology

[Close]

BLAST of MC08g_new0302 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.0e-86
Identity = 157/256 (61.33%), Postives = 190/256 (74.22%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           M+LR   +TNSI+ D+T+ DSKNFHV ++GC NLTF+   ++A   SINTDGIHIGRS G
Sbjct: 108 MNLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDG 167

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           +NI++T I TGDDCISLGDGSK + ITN+TCGPGHGISVGSLGRY  EE V GI   NCT
Sbjct: 168 VNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCT 227

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           I  + NGVRIKTWP S    G AS+MHF+D+ M +V  PILIDQ YCPYNQC  + PS V
Sbjct: 228 ITGSQNGVRIKTWPKS--EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSV 287

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+SK+SFKN++G+S    A+KLVCS   PC  VE+ +IDLTY+G  GP +S C+N+KP I
Sbjct: 288 KLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTI 347

Query: 241 SGKQNPRACSSPAPPA 257
            GKQ P  CS  A  A
Sbjct: 348 KGKQIPAICSGSAAKA 361

[Close]

BLAST of MC08g_new0302 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.0e-86
Identity = 149/256 (58.20%), Postives = 196/256 (76.56%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           M+  F+F+ NSIV  +TSKDSKNFHV + GC N+TF    + APGDS NTDGIH+G+ST 
Sbjct: 148 MNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTD 207

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           + IL+TNIGTGDDC+S+GDGSKQ+T+  + CGPGHG+SVGSLG++T EE VEGI   NCT
Sbjct: 208 VKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCT 267

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T NGVRIKTWP +P  T T SD+HFED+ M NV NP++IDQEY P+NQC+KK+PSK+
Sbjct: 268 LTATDNGVRIKTWPDAP-GTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKI 327

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+SK+SFKNV+G+S  A  + L+CS+ VPC+ VE+  +DL +NG   P +++C NVKP++
Sbjct: 328 KLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG--APTTAKCTNVKPLV 387

Query: 241 SGKQNPRACSSPAPPA 257
           +G      C +P  PA
Sbjct: 388 TG--TAPVCQAPGAPA 398

[Close]

BLAST of MC08g_new0302 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-85
Identity = 146/254 (57.48%), Postives = 196/254 (77.17%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +++RF F+TN+++ DITSKDSK FH+ +  C N+T + + + AP +S NTDGIH+G+S G
Sbjct: 150 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 209

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           +NI++++I TGDDCIS+GDG+K + I  +TCGPGHGIS+GSLG++  EEPVEGI+ +NCT
Sbjct: 210 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 269

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           I NTSNG RIKTWP      G  S++HFED+ M NVS+PILIDQ+YCP+N+C K   SKV
Sbjct: 270 ITNTSNGARIKTWPGE--HGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKV 329

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+S +SFKN+RG+SA   A+K +CS   PC++VE+ +ID+ +NG E P +SQC NVKPI 
Sbjct: 330 KLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAE-PATSQCLNVKPIT 389

Query: 241 SGKQNPRACSSPAP 255
           SGK NP  CS P P
Sbjct: 390 SGKLNPIPCSGPVP 400

[Close]

BLAST of MC08g_new0302 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 5.7e-85
Identity = 145/254 (57.09%), Postives = 195/254 (76.77%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +++RF F+TN+++ DITSKDSK FH+ +  C N+T + + + AP +S NTDGIH+G+S G
Sbjct: 150 VNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 209

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           +NI++++I TGDDCIS+GDG+K + I  +TCGPGHGIS+GSLG++  EEPVEGI+ +NCT
Sbjct: 210 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 269

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           I NTSNG RIKTWP      G  S++HFED+ M NVS+PILIDQ+YCP+N+C K   SKV
Sbjct: 270 ITNTSNGARIKTWPGE--HGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKV 329

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+S +SFKN+RG+SA   A+K +CS   PC++VE+ +ID+ +NG E P +SQC NVKPI 
Sbjct: 330 KLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAE-PATSQCLNVKPIT 389

Query: 241 SGKQNPRACSSPAP 255
            GK NP  CS P P
Sbjct: 390 IGKLNPIPCSGPVP 400

[Close]

BLAST of MC08g_new0302 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 3.8e-81
Identity = 145/252 (57.54%), Postives = 189/252 (75.00%), Query Frame = 0


Query: 2   SLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTGI 61
           +L F+ +TNS + DIT+ DSK+FHV +  C NLTF   NV+AP +S NTDGIH+ RS+ +
Sbjct: 143 NLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSV 202

Query: 62  NILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCTI 121
           NI  +N  TGDDCIS+GD ++Q+ IT +TCGPGHGISVGSLG    E+PV G+   NCT 
Sbjct: 203 NITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTF 262

Query: 122 MNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKVK 181
            NT NGVRIKTWPAS    G  +D+HFED+I+ NVSNP++IDQ YCP+N+CNK  PS+VK
Sbjct: 263 TNTDNGVRIKTWPAS--HPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVK 322

Query: 182 ISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPIIS 241
           ISKVSF+N++G+S    A+ L+ S  VPCE +EV +ID+TY+G EGP  S C+N+KP + 
Sbjct: 323 ISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIKPSLK 382

Query: 242 GKQNPRACSSPA 254
           GKQNP  C++ A
Sbjct: 383 GKQNPPVCTASA 392

[Close]

BLAST of MC08g_new0302 vs. NCBI nr
Match: XP_022157387.1 (exopolygalacturonase-like [Momordica charantia] >XP_022159278.1 exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 503 bits (1295), Expect = 3.20e-177
Identity = 251/256 (98.05%), Postives = 254/256 (99.22%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG
Sbjct: 144 MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 203

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INILSTNIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRYTAEEPVEGIRATNCT
Sbjct: 204 INILSTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 263

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           IMNTSNGVRIKTWPASPTA GTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV
Sbjct: 264 IMNTSNGVRIKTWPASPTA-GTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 323

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKNVRGSSANALA+KLVCSAEVPCEDVEV +IDLTYNGNEGPISSQCKNVKPII
Sbjct: 324 KISKVSFKNVRGSSANALAVKLVCSAEVPCEDVEVADIDLTYNGNEGPISSQCKNVKPII 383

Query: 241 SGKQNPRACSSPAPPA 256
           SGKQNPRACSSPAPPA
Sbjct: 384 SGKQNPRACSSPAPPA 398

[Close]

BLAST of MC08g_new0302 vs. NCBI nr
Match: XP_022150984.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 429 bits (1102), Expect = 9.89e-148
Identity = 207/254 (81.50%), Postives = 230/254 (90.55%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           MSL+FSFITNSIVS+ITSKDSKNFHV +LGCNNLTF+HV ++APG SINTDGIHIGRS  
Sbjct: 148 MSLKFSFITNSIVSEITSKDSKNFHVFVLGCNNLTFEHVTIDAPGHSINTDGIHIGRSNE 207

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           +N+LST IGTGDDCISLGDGSK VTI N+TCGPGHGISVGSLGRYT EEPVEGI   NCT
Sbjct: 208 VNVLSTRIGTGDDCISLGDGSKHVTINNVTCGPGHGISVGSLGRYTVEEPVEGIIVKNCT 267

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
             NT NGVRIKTWPASPT  GTA++MH+ED+IMVNVSNPILIDQEYCPYNQCNKK PSKV
Sbjct: 268 FTNTDNGVRIKTWPASPTP-GTATNMHYEDIIMVNVSNPILIDQEYCPYNQCNKKIPSKV 327

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RGSSANALA+K++CS++ PCE VE+ +IDLTYNG+EGPI+SQC NVKPII
Sbjct: 328 KISKVSFKNIRGSSANALAVKIICSSDFPCEGVEIADIDLTYNGSEGPITSQCSNVKPII 387

Query: 241 SGKQNPRACSSPAP 254
           SGKQNP ACSS AP
Sbjct: 388 SGKQNPPACSSVAP 400

[Close]

BLAST of MC08g_new0302 vs. NCBI nr
Match: XP_008459709.2 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 416 bits (1070), Expect = 5.12e-143
Identity = 199/255 (78.04%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           M+L+FSFI N+IVSDITSKDSKNFH+Q+LGCNNLTFQHVNVNAP  SINTDGIHIGRS G
Sbjct: 139 MNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINTDGIHIGRSLG 198

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INIL+TNIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRY  EEPVEG++  NCT
Sbjct: 199 INILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQVKNCT 258

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T+NG+RIKTWPASP A G ASDMH+ED++M NV NPILIDQEYCPYNQCNK+ PSKV
Sbjct: 259 LTGTTNGLRIKTWPASPAA-GVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVPSKV 318

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RG+S NA A+ LVCS+  PC+ VE+ +IDLTY+G+EGPI+S+C NVKP +
Sbjct: 319 KISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVKPTV 378

Query: 241 SGKQNPRACSSPAPP 255
           SGKQNP AC+SPA P
Sbjct: 379 SGKQNPPACTSPATP 392

[Close]

BLAST of MC08g_new0302 vs. NCBI nr
Match: KAA0039364.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK00547.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 416 bits (1070), Expect = 2.95e-142
Identity = 199/255 (78.04%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           M+L+FSFI N+IVSDITSKDSKNFH+Q+LGCNNLTFQHVNVNAP  SINTDGIHIGRS G
Sbjct: 191 MNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINTDGIHIGRSLG 250

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INIL+TNIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRY  EEPVEG++  NCT
Sbjct: 251 INILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQVKNCT 310

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T+NG+RIKTWPASP A G ASDMH+ED++M NV NPILIDQEYCPYNQCNK+ PSKV
Sbjct: 311 LTGTTNGLRIKTWPASPAA-GVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVPSKV 370

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RG+S NA A+ LVCS+  PC+ VE+ +IDLTY+G+EGPI+S+C NVKP +
Sbjct: 371 KISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVKPTV 430

Query: 241 SGKQNPRACSSPAPP 255
           SGKQNP AC+SPA P
Sbjct: 431 SGKQNPPACTSPATP 444

[Close]

BLAST of MC08g_new0302 vs. NCBI nr
Match: KAE8648971.1 (hypothetical protein Csa_008488 [Cucumis sativus])

HSP 1 Score: 404 bits (1038), Expect = 3.76e-140
Identity = 195/256 (76.17%), Postives = 223/256 (87.11%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           MSL+FSFI NS+VS ITSKDSKNFH+Q+LGCNN T Q+VNVNAPG SINTDGIH+GR  G
Sbjct: 3   MSLKFSFIKNSVVSGITSKDSKNFHIQVLGCNNFTLQNVNVNAPGTSINTDGIHLGRCVG 62

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INIL++NIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRY AEEPVEG++  NCT
Sbjct: 63  INILNSNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNAEEPVEGLQVKNCT 122

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           I  T++G+RIKTWPASP A G ASDMH+ED++M NVSNPI+IDQEYCPYN+CNK+ PSKV
Sbjct: 123 ITGTTSGLRIKTWPASPAA-GVASDMHYEDIVMNNVSNPIMIDQEYCPYNKCNKQIPSKV 182

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RG+S NA A+ LVCS+  PCE VE+ +IDLTY GNEGPI+S C NVKP I
Sbjct: 183 KISKVSFKNIRGTSGNAQAVTLVCSSSNPCEGVELADIDLTYTGNEGPITSVCANVKPTI 242

Query: 241 SGKQNPRACSSPAPPA 256
           +GKQNP  CS  AP A
Sbjct: 243 TGKQNPPICSPLAPKA 257

[Close]

BLAST of MC08g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1DUC6 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 1.55e-177
Identity = 251/256 (98.05%), Postives = 254/256 (99.22%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG
Sbjct: 144 MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 203

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INILSTNIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRYTAEEPVEGIRATNCT
Sbjct: 204 INILSTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 263

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           IMNTSNGVRIKTWPASPTA GTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV
Sbjct: 264 IMNTSNGVRIKTWPASPTA-GTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 323

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKNVRGSSANALA+KLVCSAEVPCEDVEV +IDLTYNGNEGPISSQCKNVKPII
Sbjct: 324 KISKVSFKNVRGSSANALAVKLVCSAEVPCEDVEVADIDLTYNGNEGPISSQCKNVKPII 383

Query: 241 SGKQNPRACSSPAPPA 256
           SGKQNPRACSSPAPPA
Sbjct: 384 SGKQNPRACSSPAPPA 398

[Close]

BLAST of MC08g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1DBN7 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV=1)

HSP 1 Score: 429 bits (1102), Expect = 4.79e-148
Identity = 207/254 (81.50%), Postives = 230/254 (90.55%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           MSL+FSFITNSIVS+ITSKDSKNFHV +LGCNNLTF+HV ++APG SINTDGIHIGRS  
Sbjct: 148 MSLKFSFITNSIVSEITSKDSKNFHVFVLGCNNLTFEHVTIDAPGHSINTDGIHIGRSNE 207

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           +N+LST IGTGDDCISLGDGSK VTI N+TCGPGHGISVGSLGRYT EEPVEGI   NCT
Sbjct: 208 VNVLSTRIGTGDDCISLGDGSKHVTINNVTCGPGHGISVGSLGRYTVEEPVEGIIVKNCT 267

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
             NT NGVRIKTWPASPT  GTA++MH+ED+IMVNVSNPILIDQEYCPYNQCNKK PSKV
Sbjct: 268 FTNTDNGVRIKTWPASPTP-GTATNMHYEDIIMVNVSNPILIDQEYCPYNQCNKKIPSKV 327

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RGSSANALA+K++CS++ PCE VE+ +IDLTYNG+EGPI+SQC NVKPII
Sbjct: 328 KISKVSFKNIRGSSANALAVKIICSSDFPCEGVEIADIDLTYNGSEGPITSQCSNVKPII 387

Query: 241 SGKQNPRACSSPAP 254
           SGKQNP ACSS AP
Sbjct: 388 SGKQNPPACSSVAP 400

[Close]

BLAST of MC08g_new0302 vs. ExPASy TrEMBL
Match: A0A1S3CC08 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 2.48e-143
Identity = 199/255 (78.04%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           M+L+FSFI N+IVSDITSKDSKNFH+Q+LGCNNLTFQHVNVNAP  SINTDGIHIGRS G
Sbjct: 139 MNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINTDGIHIGRSLG 198

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INIL+TNIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRY  EEPVEG++  NCT
Sbjct: 199 INILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQVKNCT 258

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T+NG+RIKTWPASP A G ASDMH+ED++M NV NPILIDQEYCPYNQCNK+ PSKV
Sbjct: 259 LTGTTNGLRIKTWPASPAA-GVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVPSKV 318

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RG+S NA A+ LVCS+  PC+ VE+ +IDLTY+G+EGPI+S+C NVKP +
Sbjct: 319 KISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVKPTV 378

Query: 241 SGKQNPRACSSPAPP 255
           SGKQNP AC+SPA P
Sbjct: 379 SGKQNPPACTSPATP 392

[Close]

BLAST of MC08g_new0302 vs. ExPASy TrEMBL
Match: A0A5A7T7B3 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001590 PE=3 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 1.43e-142
Identity = 199/255 (78.04%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           M+L+FSFI N+IVSDITSKDSKNFH+Q+LGCNNLTFQHVNVNAP  SINTDGIHIGRS G
Sbjct: 191 MNLKFSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINTDGIHIGRSLG 250

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           INIL+TNIGTGDDCISLGDGSKQVTITN+TCGPGHGISVGSLGRY  EEPVEG++  NCT
Sbjct: 251 INILNTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQVKNCT 310

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T+NG+RIKTWPASP A G ASDMH+ED++M NV NPILIDQEYCPYNQCNK+ PSKV
Sbjct: 311 LTGTTNGLRIKTWPASPAA-GVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVPSKV 370

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RG+S NA A+ LVCS+  PC+ VE+ +IDLTY+G+EGPI+S+C NVKP +
Sbjct: 371 KISKVSFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVKPTV 430

Query: 241 SGKQNPRACSSPAPP 255
           SGKQNP AC+SPA P
Sbjct: 431 SGKQNPPACTSPATP 444

[Close]

BLAST of MC08g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1HYN0 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469285 PE=3 SV=1)

HSP 1 Score: 382 bits (982), Expect = 7.03e-130
Identity = 184/252 (73.02%), Postives = 217/252 (86.11%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +SLRF+FITNSIV  ITS DSKNFH+ +LGCNNLTF +VN+ AP +S NTDGIHIGRS  
Sbjct: 149 ISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNR 208

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           I I  + I TGDDCISLGDGSKQ+ ITN+TCGPGHGIS+GSLGRYT EEPVEG+   NCT
Sbjct: 209 IAITMSKIATGDDCISLGDGSKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCT 268

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           I+NT+NGVRIKTWPAS  A+GTA++M F D+IMVNVSNPILIDQEYCP+NQCN+K PSK+
Sbjct: 269 IINTTNGVRIKTWPASA-ASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKI 328

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           KISKVSFKN+RGS A  +A+KL+CS  +PC++V+V +IDLTYNG  GPI+SQC NV+PII
Sbjct: 329 KISKVSFKNIRGSCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPII 388

Query: 241 SGKQNPRACSSP 252
           SGKQNP+ CSSP
Sbjct: 389 SGKQNPKICSSP 399

[Close]

BLAST of MC08g_new0302 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 9.0e-86
Identity = 146/253 (57.71%), Postives = 195/253 (77.08%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +S+RF FI NS + DI+S D+KNFH+ +LG  N+T  ++ + AP DS NTDGIH+GRS G
Sbjct: 139 ISIRFDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDG 198

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           + IL++ I TGDDCIS+GDG K + +  +TCGPGHGISVGSLGRY  E+ V GI+  NCT
Sbjct: 199 VKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCT 258

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T NG+RIKTWP++  +T TASD+HFED+I+ +VSNPILIDQEYCP+NQCNK+  S +
Sbjct: 259 LQETDNGLRIKTWPSAACST-TASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTI 318

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+  +SFKN+RG+S N  A+KL+CS   PC++VE+ +ID+ YNG +GP +  C NV P I
Sbjct: 319 KLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKI 378

Query: 241 SGKQNPRACSSPA 254
            G Q+P+ACS+PA
Sbjct: 379 LGSQSPKACSAPA 390

[Close]

BLAST of MC08g_new0302 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.1e-83
Identity = 142/257 (55.25%), Postives = 196/257 (76.26%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +S+RF F+T++ +  ITS D+K+FH+ ++G  N+TF+ V + AP +S NTDGIH+GRS G
Sbjct: 139 ISIRFDFVTDAKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDG 198

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           I I+++ I TGDDC+S+GDG K + +  +TCGPGHGIS+GSLGRY+ EE V GI+  NCT
Sbjct: 199 IKIINSFISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCT 258

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T NG+RIKTWP S   T TASD+HFE++++ NVSNPILIDQEYCP+NQCNK+ PS +
Sbjct: 259 LQETDNGLRIKTWP-SAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTI 318

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K++ +SFK +RG+S N  A+KL+CS   PC++VEV ++++ Y G +GP + QC NV P +
Sbjct: 319 KLANISFKKIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKL 378

Query: 241 SGKQNPRACSSPA--PP 256
            G Q P+ACSSP   PP
Sbjct: 379 VGTQIPKACSSPVTKPP 394

[Close]

BLAST of MC08g_new0302 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 1.9e-83
Identity = 145/255 (56.86%), Postives = 191/255 (74.90%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +S+RF F+ N+ + DI+S D+KNFH+ +LG  N+TF +V V AP +S NTDGIH+GRS G
Sbjct: 140 ISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEG 199

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           + IL++ I TGDDCIS+GDG K + + N+ CGPGHGISVGSLGRY  E+ V GI   NCT
Sbjct: 200 VKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCT 259

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T NG+RIKTWP++  AT TAS +HFE++I+ NVSNPILIDQEYCP+NQCNK+ PS +
Sbjct: 260 LQGTDNGLRIKTWPSAACAT-TASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTI 319

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+  +SFKN+RG+S N  A+KL+CS   PC +VE+  I+L Y G +GP +  C NV P +
Sbjct: 320 KLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKL 379

Query: 241 SGKQNPRACSSPAPP 256
            G QNP+ACS+   P
Sbjct: 380 VGTQNPKACSAGPTP 393

[Close]

BLAST of MC08g_new0302 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.2e-82
Identity = 136/252 (53.97%), Postives = 195/252 (77.38%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +S+RF F+TN+ + DI+S D+KNFH+ ++G  N+TF +V + AP +S NTDGIH+GRS G
Sbjct: 140 ISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVG 199

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           ++I+++ I TGDDC+S+GDG   + + N+ CGPGHGISVGSLGRY  E+ V GIR  NCT
Sbjct: 200 VSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCT 259

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           +  T NG+RIKTWP++  +T TAS++HFE++I+ NVSNPILIDQEYCP+NQCNK+  S +
Sbjct: 260 LQETDNGLRIKTWPSAACST-TASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSI 319

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K++ +SF+ +RG+S N  A+KL+CS   PCE+V+V +I++ Y G +GP +  C NV+P +
Sbjct: 320 KLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKL 379

Query: 241 SGKQNPRACSSP 253
            G Q P+AC++P
Sbjct: 380 VGTQFPKACNTP 390

[Close]

BLAST of MC08g_new0302 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 2.5e-80
Identity = 138/255 (54.12%), Postives = 188/255 (73.73%), Query Frame = 0


Query: 1   MSLRFSFITNSIVSDITSKDSKNFHVQILGCNNLTFQHVNVNAPGDSINTDGIHIGRSTG 60
           +++RF+ +TNS ++ ITS +SK FH+ IL C N+T   + ++AP +S+NTDGIHIGRS G
Sbjct: 191 INIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNG 250

Query: 61  INILSTNIGTGDDCISLGDGSKQVTITNMTCGPGHGISVGSLGRYTAEEPVEGIRATNCT 120
           +N++   I TGDDC+S+GDG++ + + N+ CGPGHGIS+GSLGRY  E+PV+G+    C 
Sbjct: 251 VNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCL 310

Query: 121 IMNTSNGVRIKTWPASPTATGTASDMHFEDMIMVNVSNPILIDQEYCPYNQCNKKSPSKV 180
           I NT NGVRIKTWP SP   G AS++ FED+ M NVS P+LIDQEYCPY  C    PSKV
Sbjct: 311 IKNTDNGVRIKTWPGSP--PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKV 370

Query: 181 KISKVSFKNVRGSSANALALKLVCSAEVPCEDVEVVEIDLTYNGNEGPISSQCKNVKPII 240
           K+S V+ KN++G+SA  +A+KL+CS  VPC ++ + +I+L +NG EGP  S C N+KPI+
Sbjct: 371 KLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPIL 430

Query: 241 SGKQNPRACSSPAPP 256
           SGK  P AC+  A P
Sbjct: 431 SGKLVPAACTEVAKP 443

The following BLAST results are available for this feature:

BLAST of MC08g_new0302 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24548	1.0e-86	61.33	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q40312	3.0e-86	58.20	Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1	[more]
Q39786	1.5e-85	57.48	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
Q39766	5.7e-85	57.09	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
Q05967	3.8e-81	57.54	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]

BLAST of MC08g_new0302 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157387.1	3.20e-177	98.05	exopolygalacturonase-like [Momordica charantia] >XP_022159278.1 exopolygalacturo...	[more]
XP_022150984.1	9.89e-148	81.50	exopolygalacturonase-like [Momordica charantia]	[more]
XP_008459709.2	5.12e-143	78.04	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
KAA0039364.1	2.95e-142	78.04	exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK00547.1 exopolygalactur...	[more]
KAE8648971.1	3.76e-140	76.17	hypothetical protein Csa_008488 [Cucumis sativus]	[more]

BLAST of MC08g_new0302 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUC6	1.55e-177	98.05	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111024096 PE=3 SV...	[more]
A0A6J1DBN7	4.79e-148	81.50	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111019009 PE=3 SV...	[more]
A0A1S3CC08	2.48e-143	78.04	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103498752 PE=3 SV=1	[more]
A0A5A7T7B3	1.43e-142	78.04	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1HYN0	7.03e-130	73.02	exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469285 PE=3 SV=1	[more]

BLAST of MC08g_new0302 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07820.1	9.0e-86	57.71	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	1.1e-83	55.25	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	1.9e-83	56.86	Pectin lyase-like superfamily protein	[more]
AT3G07830.1	1.2e-82	53.97	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	2.5e-80	54.12	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 31..57 e-value: 270.0 score: 10.4 coord: 81..101 e-value: 5300.0 score: 2.1 coord: 111..132 e-value: 13.0 score: 14.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 2..237 e-value: 6.6E-79 score: 265.3
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 88..101
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..252 e-value: 4.1E-76 score: 258.6
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..252
None	No IPR available	PANTHER	PTHR31375:SF248	POLYGALACTURONASE	coord: 2..252
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..250

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0302.1	MC08g_new0302.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity