MC08g_new0301 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0301
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-isopropylmalate synthase
Location	MC08: 16011988 .. 16014194 (-)
RNA-Seq Expression	MC08g_new0301
Synteny	MC08g_new0301

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCAATCACAAAAGTACTTATGAAATCATATCCCCTGAAGATATTGGCTATGAAGGATCCAATGATGTTGGTATTGTTCCAGGAAAACTTAGGTATGTGTAAGCATGTGCATTTTGAGCCCGGTTAGAATACAGTTTTGTATCAAATAGGTCATTGACCTACTTGAAATTTTTCAAAATTCATGAACCTATTAAATACAAGATTGTAAAATTTTAATTTCTATTAAAATTTCGACATTATAACCATCGATTATGCGTTTTTATTAGCGTGGCTGAGGTTATAAAGATATAAAATGATACCTTTTCATTGTTTTCATTGGAATCTAAAGTAGTGTTGAACTTGAAATTATTTTGTGCCAAACACGGGAGGGGATTAGAAATCACAACCTCTTGATTTGGAGGTATATGCCAATTACCGCTGAGTTATGCTCGCTTTGGCATATATGTAGGAGGAGTTGCAGGTATAATAATCTGGTTGAAAACAACATCAAATTAAGCACAAGCTAAAAAAAAATTTAAAATATATAGCACAACTTTTAAGTGCAACTTAATAAGATCAATTTACCACATGTCTATCCGTGATAGATGTTTGTCACTGGTTTATATCTGTCAATCTATTATAGTGATAAATAATTGTACCATATACAAATGATCATATTTACGAGATCTTATTTCTTATACTGCGTTTATAAATATGGCAAAACTATTTACTACTTATTTTGTCCGAAATATAGTATATCCCATAAGTAACATGGTTCATTATGACAACTCTTACAGTGGACGTCATGCCCTCAAAAGTCAACTTCGAGAGGTAAATTGGGATTTCCAGGCTCAATATTAAGGTAATTTTTTATGTATTTAAAATTTTAATGTTATATTACCTATTTTCAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAAGATATTCCAACGTTTCGAAGCAGTGGCTGAACAGAAGAAGGTAAATTATTGTTCATCAAGAAAGTTTTTCATTTAATTCAATGCTTTTATTCCTAATTGTCTAAATGTTGCTCTATTTTCTTTTTTTCTTTTTAACATTCCAGAGAGTTACGGATGCCGATCTTCGAGCATTGGTATCTGATGAAGCTTTTCAACCATGTAGCGTTTGGAAGATGCTTGATTTGCAGGTTTAAGGATTTTCTTATAGATCGTCATTTTTCGAGAACTTGTTTGAACCAATTTAAATAAGTTTTTTTTTTTTTTTTTGCGTACAATACGTGGGAGTGAGGGATCGAATTATGCTAATAACCGCTGAGCTATGTTCGCTTTAACTTACACTTTGTAATGTTTAAAAATATATTAGACATAACCCGAAGAATTTATGGGTTAAACTTGTAATTTAACCGTTTTATTTTACAATACCTCAAATGGCAATGGCAGGTAAGCTGTGGAACTTTTGGTGTTTCTACTGCTACTATTAAACTCCTTGACGGTAAGGGGAAAGAGCATATTTCTTGTTCGGTTGGAACTGGTCCTATAGATTCGGCTTACAAGGCTGTAGATCTCATTGTGAAGGTATGTGATCATTCAATCAAACTTTTTATAGTTCCCATTTAACAATTTTCATTTGGAGTGCATTTTCCTTTTGGATTATTTCCTGAGAAATCCTTTTTTATTTTTTATTTTTTATTTTTTTTAATGCAGAAACCAGTTAGGCTTCTCGAGTATTCGATGAATGCAGTCACGGAAGGCATTGATGCAATTGTCACCACCCGTGTTGTAATAAGATCGGAAAACAGTTACACATTTTGGTAACTAAGCCGAGTTTGTTAACCATTTTGTTTTTTTTTTTCATTTTTTAAAAGTTAACTACTATTTCTCTCTATGCATCTTTTTTTTTTGGTTTTTTTTAATTATCTACTTTTTGAAAACTTTGTTAAAATCTAAGCCAATATTTTAAAAACCAAGGAGTAGTTTTTTTTAAAAATAGCAATTTCTTTATTTTATTTTGTGTTTTCTTTTCTATAATTTGGCTAAAAACTCGAATGTGAAGAAAAAAAAAGAAATAATGTATAGAATTTTTAAACATAGAAAGCTACAAAATGAAATCATTATCAAACGAGACTCTAGTGATTTGTTTTTATTATTGCAGTGGTATCGGGGCATAGTCGTATCGAGTGTAAAGGCCTACGTTGGTGCGCTGAACAAGATGCTTTGGTTTCCAAAGAGTGGACATTAA

mRNA sequence

ATGCTCAATCACAAAAGTACTTATGAAATCATATCCCCTGAAGATATTGGCTATGAAGGATCCAATGATGTTGGTATTGTTCCAGGAAAACTTAGTGGACGTCATGCCCTCAAAAGTCAACTTCGAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAAGATATTCCAACGTTTCGAAGCAGTGGCTGAACAGAAGAAGAGAGTTACGGATGCCGATCTTCGAGCATTGGTATCTGATGAAGCTTTTCAACCATGTAGCGTTTGGAAGATGCTTGATTTGCAGGTAAGCTGTGGAACTTTTGGTGTTTCTACTGCTACTATTAAACTCCTTGACGGTAAGGGGAAAGAGCATATTTCTTGTTCGGTTGGAACTGGTCCTATAGATTCGGCTTACAAGGCTGTAGATCTCATTGTGAAGAAACCAGTTAGGCTTCTCGAGTATTCGATGAATGCAGTCACGGAAGGCATTGATGCAATTGTCACCACCCGTGTTTGGTATCGGGGCATAGTCGTATCGAGTGTAAAGGCCTACGTTGGTGCGCTGAACAAGATGCTTTGGTTTCCAAAGAGTGGACATTAA

Coding sequence (CDS)

ATGCTCAATCACAAAAGTACTTATGAAATCATATCCCCTGAAGATATTGGCTATGAAGGATCCAATGATGTTGGTATTGTTCCAGGAAAACTTAGTGGACGTCATGCCCTCAAAAGTCAACTTCGAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAAGATATTCCAACGTTTCGAAGCAGTGGCTGAACAGAAGAAGAGAGTTACGGATGCCGATCTTCGAGCATTGGTATCTGATGAAGCTTTTCAACCATGTAGCGTTTGGAAGATGCTTGATTTGCAGGTAAGCTGTGGAACTTTTGGTGTTTCTACTGCTACTATTAAACTCCTTGACGGTAAGGGGAAAGAGCATATTTCTTGTTCGGTTGGAACTGGTCCTATAGATTCGGCTTACAAGGCTGTAGATCTCATTGTGAAGAAACCAGTTAGGCTTCTCGAGTATTCGATGAATGCAGTCACGGAAGGCATTGATGCAATTGTCACCACCCGTGTTTGGTATCGGGGCATAGTCGTATCGAGTGTAAAGGCCTACGTTGGTGCGCTGAACAAGATGCTTTGGTTTCCAAAGAGTGGACATTAA

Protein sequence

MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRFEAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHISCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRGIVVSSVKAYVGALNKMLWFPKSGH

Homology

[Close]

BLAST of MC08g_new0301 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 275.4 bits (703), Expect = 4.9e-73
Identity = 143/219 (65.30%), Postives = 164/219 (74.89%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEII PE+IG E SND GIV GKLSGRHALK +L ELGY+LD+E L  IF RF
Sbjct: 399 MLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQLSTIFWRF 458

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           + VAEQKKRVTDAD+ ALVSDE FQP +VWK+LD+Q++CGT G+STAT+KL D  GKEH+
Sbjct: 459 KTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKEHV 518

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CS+GTGP+DSAYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 519 ACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAI 578

Query: 181 -----------------IVVSSVKAYVGALNKMLWFPKS 193
                            IVVSSVKAYVGALNKM+ F ++
Sbjct: 579 TGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFKEN 617

[Close]

BLAST of MC08g_new0301 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 2.4e-72
Identity = 143/216 (66.20%), Postives = 162/216 (75.00%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEI+SPE+IG E SND GIV GKLSGRHALK +L ELGY LD+  L  +F RF
Sbjct: 397 MLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRF 456

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADL ALVSDE FQP +VWK+LD+Q++CGT G+ST+T+KL D  GKEH+
Sbjct: 457 KAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKEHV 516

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CSVGTGP+D+AYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 517 ACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAV 576

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 190
                            IVVSSVKAYVGALNKML F
Sbjct: 577 TGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGF 612

[Close]

BLAST of MC08g_new0301 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-63
Identity = 124/218 (56.88%), Postives = 160/218 (73.39%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISP+D+G   SN+ GIV GKLSGRHALKS++ ELGY++D + L+ +F RF
Sbjct: 358 MLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSGRHALKSKMLELGYDIDGKELEDLFWRF 417

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           ++VAE+KK++TD DL AL+SDE  QP   WK+ D+Q+ CG+ G+STAT+KL++  G+EHI
Sbjct: 418 KSVAEKKKKITDDDLIALMSDEVLQPNVYWKLGDVQIMCGSLGLSTATVKLINTDGQEHI 477

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRV-------------- 180
           +CSVGTGP+D+AYKAVDLIVK P+ LLEYSMNAVTEGIDAI +TRV              
Sbjct: 478 ACSVGTGPVDAAYKAVDLIVKVPITLLEYSMNAVTEGIDAIASTRVSICSIDRHTIMNGS 537

Query: 181 -------WYRG------IVVSSVKAYVGALNKMLWFPK 192
                   + G      +V+SSV+AY+GALNKML + K
Sbjct: 538 TGQTIHRTFSGTGADMDVVISSVRAYIGALNKMLSYEK 575

[Close]

BLAST of MC08g_new0301 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 1.6e-63
Identity = 127/218 (58.26%), Postives = 158/218 (72.48%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIG   +N+ GIV GKLSG    K ++ ELGYE++ + LD +F RF
Sbjct: 381 MLKHKDTYEIISPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRF 440

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           ++VAE+KK++TD DL AL+SDE FQP  VW++ ++QV+CG+ G+STAT+KL+D  G+EHI
Sbjct: 441 KSVAEKKKKITDDDLVALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGREHI 500

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           SCSVGTGP+D+AYKAVDLIVK PV LLEYSMNAVT+GIDAI +TRV  RG          
Sbjct: 501 SCSVGTGPVDAAYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHAL 560

Query: 181 -----------------IVVSSVKAYVGALNKMLWFPK 192
                            IV+SSV+AYVGALNKM+ F K
Sbjct: 561 TGETVHRTFSGTGADMDIVISSVRAYVGALNKMMSFRK 598

[Close]

BLAST of MC08g_new0301 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.7e-53
Identity = 110/219 (50.23%), Postives = 151/219 (68.95%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYE ISPE+IG++ +  +GIV GKLSG  AL+ +L ELGY+L  + +D +F +F
Sbjct: 343 MLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVFWQF 402

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +A+AE+KK VTD DL+ALVS +AF   S+WK+ DLQV+CGT G+STAT+KL++  G  H+
Sbjct: 403 KAMAEKKKVVTDVDLKALVSYKAFHAESIWKLGDLQVTCGTIGLSTATVKLVNIDGSTHV 462

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRV-------------- 180
           +CS+G G +DS YKA++LIVK+P +LL+YS+N+VTEGI   VT RV              
Sbjct: 463 ACSIGIGAVDSTYKAINLIVKEPTKLLDYSLNSVTEGIGVNVTARVVICRENNHTSTYAF 522

Query: 181 -------WYRGI------VVSSVKAYVGALNKMLWFPKS 193
                   + GI      VVS+VKAY+ ALNK+L + +S
Sbjct: 523 TEDANYPTFSGIAAEMDVVVSTVKAYLVALNKLLRWKES 561

[Close]

BLAST of MC08g_new0301 vs. NCBI nr
Match: XP_022143334.1 (2-isopropylmalate synthase 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 293 bits (750), Expect = 6.85e-98
Identity = 156/216 (72.22%), Postives = 168/216 (77.78%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SND GIV GKLSGRHALKS+L+ELGYELDNENLD IF RF
Sbjct: 1   MLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRF 60

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           + VAEQKKRVTDADLRALVSDE FQP  VWK+LDLQV+CGT G+STAT+KLLD  GKEHI
Sbjct: 61  KEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHI 120

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVD IVK+PV LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 121 NCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAM 180

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IV SSVKAY+GALNKML F
Sbjct: 181 TGEAVQRTFSGIGAGMDIVXSSVKAYIGALNKMLGF 216

[Close]

BLAST of MC08g_new0301 vs. NCBI nr
Match: KAF3946191.1 (hypothetical protein CMV_027513, partial [Castanea mollissima])

HSP 1 Score: 282 bits (721), Expect = 2.03e-93
Identity = 148/218 (67.89%), Postives = 167/218 (76.61%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEII+PEDIGYE SN+ GIV GKLSGRHAL+ +L ELGYELDNE L+ IF RF
Sbjct: 3   MLKHKGTYEIIAPEDIGYERSNEAGIVLGKLSGRHALRKRLEELGYELDNEQLENIFWRF 62

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVA+QKKRVTDADLRALVSDE FQP  VWK+ DLQV+CGT G+STAT+KL+D  G EH+
Sbjct: 63  KAVADQKKRVTDADLRALVSDEVFQPEVVWKLHDLQVTCGTLGLSTATVKLIDADGTEHV 122

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CSVGTGP+DSAYKAVDLIVK+PV LLEYSM+AVTEGIDAI TTRV  RG          
Sbjct: 123 ACSVGTGPVDSAYKAVDLIVKEPVTLLEYSMSAVTEGIDAIATTRVLIRGENSHTSTHAY 182

Query: 181 -----------------IVVSSVKAYVGALNKMLWFPK 191
                            IVVSSVKAY+GALNKML F +
Sbjct: 183 TGETVHRTFSGIGAGMDIVVSSVKAYIGALNKMLGFKE 220

[Close]

BLAST of MC08g_new0301 vs. NCBI nr
Match: XP_022141882.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 293 bits (750), Expect = 6.39e-93
Identity = 156/216 (72.22%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SND GIV GKLSGRHALKS+L+ELGYELDNENLD IF RF
Sbjct: 392 MLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRF 451

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  VWK+LDLQV+CGT G+STAT+KLL+  GKEH 
Sbjct: 452 KAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHT 511

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVD IVK+PV LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 512 NCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAI 571

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 572 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 607

[Close]

BLAST of MC08g_new0301 vs. NCBI nr
Match: XP_022141881.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 293 bits (750), Expect = 7.78e-93
Identity = 156/216 (72.22%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SND GIV GKLSGRHALKS+L+ELGYELDNENLD IF RF
Sbjct: 392 MLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRF 451

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  VWK+LDLQV+CGT G+STAT+KLL+  GKEH 
Sbjct: 452 KAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHT 511

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVD IVK+PV LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 512 NCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAI 571

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 572 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 607

[Close]

BLAST of MC08g_new0301 vs. NCBI nr
Match: XP_038875433.1 (2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 293 bits (749), Expect = 1.34e-92
Identity = 154/216 (71.30%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SN+ GIV GKLSGRHALKS+L ELGYELD+ENLD +F RF
Sbjct: 400 MLKHKGTYEIISPEDIGYERSNEAGIVLGKLSGRHALKSRLLELGYELDSENLDNVFWRF 459

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  +WK+LDLQV+CGT G+STAT+KLLD  GKEHI
Sbjct: 460 KAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHI 519

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 520 ACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTATNAL 579

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 580 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 615

[Close]

BLAST of MC08g_new0301 vs. ExPASy TrEMBL
Match: A0A6J1CP08 (2-isopropylmalate synthase 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111013233 PE=4 SV=1)

HSP 1 Score: 293 bits (750), Expect = 3.32e-98
Identity = 156/216 (72.22%), Postives = 168/216 (77.78%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SND GIV GKLSGRHALKS+L+ELGYELDNENLD IF RF
Sbjct: 1   MLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRF 60

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           + VAEQKKRVTDADLRALVSDE FQP  VWK+LDLQV+CGT G+STAT+KLLD  GKEHI
Sbjct: 61  KEVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLDADGKEHI 120

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVD IVK+PV LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 121 NCAVGTGPVDSAYKAVDGIVKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAM 180

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IV SSVKAY+GALNKML F
Sbjct: 181 TGEAVQRTFSGIGAGMDIVXSSVKAYIGALNKMLGF 216

[Close]

BLAST of MC08g_new0301 vs. ExPASy TrEMBL
Match: A0A6J1CL37 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 293 bits (750), Expect = 3.09e-93
Identity = 156/216 (72.22%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SND GIV GKLSGRHALKS+L+ELGYELDNENLD IF RF
Sbjct: 392 MLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRF 451

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  VWK+LDLQV+CGT G+STAT+KLL+  GKEH 
Sbjct: 452 KAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHT 511

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVD IVK+PV LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 512 NCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAI 571

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 572 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 607

[Close]

BLAST of MC08g_new0301 vs. ExPASy TrEMBL
Match: A0A6J1CKJ3 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 293 bits (750), Expect = 3.77e-93
Identity = 156/216 (72.22%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEIISPEDIGYE SND GIV GKLSGRHALKS+L+ELGYELDNENLD IF RF
Sbjct: 392 MLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRF 451

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  VWK+LDLQV+CGT G+STAT+KLL+  GKEH 
Sbjct: 452 KAVAEQKKRVTDADLRALVSDEVFQPSVVWKLLDLQVTCGTLGLSTATVKLLNADGKEHT 511

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +C+VGTGP+DSAYKAVD IVK+PV LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 512 NCAVGTGPVDSAYKAVDGIVKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAI 571

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 572 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 607

[Close]

BLAST of MC08g_new0301 vs. ExPASy TrEMBL
Match: A0A0A0L7C8 (2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 291 bits (744), Expect = 3.88e-92
Identity = 153/216 (70.83%), Postives = 167/216 (77.31%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEI++PEDIGYE SND GIV GKLSGRHALKS L ELGYELD ENLD +F RF
Sbjct: 403 MLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRF 462

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  +WK+LD+QV+CGT G+STAT+KLLD  GKEHI
Sbjct: 463 KAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHI 522

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CSVGTGP+DSAYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 523 ACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNAL 582

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 583 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 618

[Close]

BLAST of MC08g_new0301 vs. ExPASy TrEMBL
Match: A0A5D3BP53 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00350 PE=3 SV=1)

HSP 1 Score: 290 bits (743), Expect = 5.34e-92
Identity = 153/216 (70.83%), Postives = 168/216 (77.78%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEI++PEDIGYE SND GIV GKLSGRHALKS+L ELGYELD+ENL  +F RF
Sbjct: 402 MLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRF 461

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADLRALVSDE FQP  +WK+LDLQV+CGT G+STAT+KLLD  GKEHI
Sbjct: 462 KAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHI 521

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CSVGTGP+DSAYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 522 ACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNAL 581

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 189
                            IVVSSVKAY+GALNKML F
Sbjct: 582 TGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 617

[Close]

BLAST of MC08g_new0301 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 275.4 bits (703), Expect = 3.5e-74
Identity = 143/219 (65.30%), Postives = 164/219 (74.89%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEII PE+IG E SND GIV GKLSGRHALK +L ELGY+LD+E L  IF RF
Sbjct: 399 MLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQLSTIFWRF 458

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           + VAEQKKRVTDAD+ ALVSDE FQP +VWK+LD+Q++CGT G+STAT+KL D  GKEH+
Sbjct: 459 KTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQITCGTLGLSTATVKLADADGKEHV 518

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CS+GTGP+DSAYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 519 ACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAI 578

Query: 181 -----------------IVVSSVKAYVGALNKMLWFPKS 193
                            IVVSSVKAYVGALNKM+ F ++
Sbjct: 579 TGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDFKEN 617

[Close]

BLAST of MC08g_new0301 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 273.1 bits (697), Expect = 1.7e-73
Identity = 143/216 (66.20%), Postives = 162/216 (75.00%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           ML HK TYEI+SPE+IG E SND GIV GKLSGRHALK +L ELGY LD+  L  +F RF
Sbjct: 397 MLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRF 456

Query: 61  EAVAEQKKRVTDADLRALVSDEAFQPCSVWKMLDLQVSCGTFGVSTATIKLLDGKGKEHI 120
           +AVAEQKKRVTDADL ALVSDE FQP +VWK+LD+Q++CGT G+ST+T+KL D  GKEH+
Sbjct: 457 KAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKEHV 516

Query: 121 SCSVGTGPIDSAYKAVDLIVKKPVRLLEYSMNAVTEGIDAIVTTRVWYRG---------- 180
           +CSVGTGP+D+AYKAVDLIVK+P  LLEYSMNAVTEGIDAI TTRV  RG          
Sbjct: 517 ACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAV 576

Query: 181 -----------------IVVSSVKAYVGALNKMLWF 190
                            IVVSSVKAYVGALNKML F
Sbjct: 577 TGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGF 612

[Close]

BLAST of MC08g_new0301 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-21
Identity = 50/84 (59.52%), Postives = 66/84 (78.57%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           +L ++STYEI+SPEDIG   S + G+V GKLSGRHA+K +L+ELGYELD+E L+ +F  F
Sbjct: 396 ILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLF 455

Query: 61  EAVAEQKKRVTDADLRALV--SDE 83
             + + KKR+TDADL+ALV  SDE
Sbjct: 456 RDLTKNKKRITDADLKALVTSSDE 479

[Close]

BLAST of MC08g_new0301 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 46/83 (55.42%), Postives = 64/83 (77.11%), Query Frame = 0


Query: 1   MLNHKSTYEIISPEDIGYEGSNDVGIVPGKLSGRHALKSQLRELGYELDNENLDKIFQRF 60
           +L ++STYEI+SPED+G   S + GIV GKLSGRHA+K +L+ELGYE+ +E  + IF R+
Sbjct: 396 ILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFNDIFSRY 455

Query: 61  EAVAEQKKRVTDADLRALVSDEA 84
             + + KKR+TDADL+ALV + A
Sbjct: 456 RELTKDKKRITDADLKALVVNGA 478

The following BLAST results are available for this feature:

BLAST of MC08g_new0301 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPR4	4.9e-73	65.30	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9C550	2.4e-72	66.20	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	1.2e-63	56.88	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	1.6e-63	58.26	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	5.7e-53	50.23	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of MC08g_new0301 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143334.1	6.85e-98	72.22	2-isopropylmalate synthase 2, chloroplastic-like [Momordica charantia]	[more]
KAF3946191.1	2.03e-93	67.89	hypothetical protein CMV_027513, partial [Castanea mollissima]	[more]
XP_022141882.1	6.39e-93	72.22	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Momordica charantia...	[more]
XP_022141881.1	7.78e-93	72.22	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Momordica charantia...	[more]
XP_038875433.1	1.34e-92	71.30	2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida]	[more]

BLAST of MC08g_new0301 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP08	3.32e-98	72.22	2-isopropylmalate synthase 2, chloroplastic-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CL37	3.09e-93	72.22	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A6J1CKJ3	3.77e-93	72.22	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A0A0L7C8	3.88e-92	70.83	2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1	[more]
A0A5D3BP53	5.34e-92	70.83	2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of MC08g_new0301 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18500.1	3.5e-74	65.30	methylthioalkylmalate synthase-like 4	[more]
AT1G74040.1	1.7e-73	66.20	2-isopropylmalate synthase 1	[more]
AT5G23010.1	1.5e-21	59.52	methylthioalkylmalate synthase 1	[more]
AT5G23020.1	1.0e-20	55.42	2-isopropylmalate synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	SMART	SM00917	LeuA_dimer_2	coord: 69..187 e-value: 1.4E-18 score: 77.8
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	PFAM	PF08502	LeuA_dimer	coord: 70..186 e-value: 2.9E-26 score: 91.9
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	GENE3D	3.30.160.270		coord: 95..187 e-value: 3.2E-13 score: 51.7
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	SUPERFAMILY	110921	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	coord: 93..186
None	No IPR available	GENE3D	1.10.238.260		coord: 1..92 e-value: 5.4E-20 score: 73.8
None	No IPR available	PANTHER	PTHR10277:SF65	2-ISOPROPYLMALATE SYNTHASE A-LIKE ISOFORM X1	coord: 1..170
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 1..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0301.1	MC08g_new0301.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016020	membrane
molecular_function	GO:0003852	2-isopropylmalate synthase activity