MC08g_new0227 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0227
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	alpha-dioxygenase 1-like
Location	MC08: 12278373 .. 12280903 (+)
RNA-Seq Expression	MC08g_new0227
Synteny	MC08g_new0227

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CATAGAGAGAGGAGAGAGAGAAAATGATTTCTCAGTTACTGAGATCAGAGCTTCAAAAGTTCATCCATAAAGATTTTCATGAAGTTGTTCAGAGGATGACTGTTATCGACACTCTGCTCTTCTTGGTAACGGTTTTTCATTTTTGGTTTTTCTGTTAAGTTAAAAGCTTTGTTTACAAATTGTTTTATGTCATATTACAACTTTTCAAAAGCGAAATATTTGTTGAACGAAACGATAATATTTGGTAACGCTTATAATTTCTTACGGTCGGTTTGGTAGTGTTTTTCTTTCTCTCTTTTTTTTTTCATTTCGATTCTTGTACTTTTACAAATAAGTCATTTTTGTCGTTATTTTCAATATTTTTACACAATAATTCTACACATGACTAAAATCATTTAATGTAAATTCATGGTTACATTTGACGAATAAAAATGTTGAGTATAGTGTCATGTTGGAATTGTGATAAAAAATGAAATTAAAAATAAAAGTAAAAGACCAAAATGGTCACTTTTTAAAAGTTAAAGTGACCAAAATAAACATTTAGAAAATATAGGAACCAAAATAATCAAAGTTGAAAGTATAAAGTTCAAAATAAACATTTTAAAGGTACAGGGACCAACCTGAACCAAAACTAAAAAGTATATTTTAACCTTATATACTTATTTTTTTAAACATTTATTATGTATTTCCGACCCTAATAAAATAATGTTTCTATTATAATTTTGTTTATAAAAAAAAAAAGAGAGAAAACTAAAAATTAAAAATAAGAAACTTGATTTTATTGTTTCAAAAGTTTTACATAATTTTGAAAATATTTCTTAAAAATGAGAAATATAAACCTTTATCGAACATTTATTCTTCTTAAAAATTAAAAATGAGAAATAACAACATGGCTGGAAAATAATATTACCAAACCGCAATACCTTTCTTATTTTTTATTTTATGTGCTTTGGTTTTCTATTCTCGAGTAGAGAAAAACAAAATGGTGTTCATGATTTCTTTTTGTTGTTGGTTTCTTCTACATCCTTATTATGGTAAAAAGGAAACTAAATTGGTTTGGATGACAATTTAAAATTGAAAAAAAAAAGTAAAGTTTTACCGTAAAACAATTTTTAATAATAATAAATTTAAAACAATGACGTTATATTTTAGATCTTATTTATTATGTATTTTATGAATACAAAATAGTATTTATGTTTTTGAAAAGGTAGAAAGAAGTACAAGAAAACAAAAAATAGTAACCAAAATTTTTTTGTTTTCTTTAATTTATTTACAATTTTGAAAGCAATTTTTACCGATAAAAAACGAGAAATGGATCTTTAACTATTACTACCAAACATTTAAGGTGTGCATATGTCGGCATTAGTTTTGTACTTAAATCGAACCGACGTCGTTCTTTAACCATTTTATTTTTGTCGGTCAAAAACCGTCAATTTTGGAAGCTTTGTAACGACTTCGAACATATAATCGCGGTTGGTCAATTTTGGCTAAAACCAACCTAAGATTGAGCCATGCAAAGACTAAATGAGGTGAACAAAGAAGAAAAAGAAACTTGTGAGGAAGAAATGAAGAGGGGAGAGAAGCAAGAAGAGAAAAGAAGAGGTAGTGAGAAAAGGAAGAAGAAGGGGAGAGGAGGGGGGAGAGTGAAATGATAGGTTTTTTTTTTTCTTTTTTAAGGAAAACTTTTTTTTTATTTAATGTACAAAGTTTCTAGAAGACTACAAATACTCGAAAAAAATAGTGGTACCACTCATTAGACTGACTAATTAACATTGATTTGATGGGTCGATTTTTCAGTAATATGTTCATCCCTAATAAATGCATGCTTATATCAAAAAATTGAAAATAATGAATTGAAAATAATGAAACATTAAAGAAATACGCTCACAAACAACCTCTAACATTCGAATCACATTTTTTTTTTTTTGTAGATTGTGCATTTGATTGATAAGTTGGGAGTTTGGCATAAACTACCAGTGATTTTGGGACTAATTTATATAGCAATTCGTCGTCATATTCATCAAGAATATAACTTGTTCAATGTCGGTAGAACTCCGGTCGGAGTTCGGTTCAACTCAGTGGATTTTCCATACCGAACTGCTAATGGCAAGTACAATGATCCCTTCAATGAAGCTGTTGGTGCCCAAGGATCCTTCTTCGGTAGAAACATCCTCCCCATCGACCAAACCAAAAGGGTAACATACAAAAAATACACAACATTCACTTGTTATTCTAGGTGATTATTGAGTTCGAAGTTTAAAAACTCATTTGATAACGTTCTTGTAGTTATTGAAACCCAACCCAATGGTGGTTGCTACAAAGCTTCTGGCAAGGAAAAAGCTTATAGACACTGGAAAACAATTCAACATGATTGCTGTTTTTTGGATTCAGTTCATGATTCATGATTGGATGGATCATTTAGAGGATACCAAGCAGATCCAATACATATATATATATATAATATTCTTCTTAATTATTTAATTTAATCCAAATTGAAGTTTTAGAAATCAACATGTGAAAATGAAAGGTGG

mRNA sequence

ATGATTTCTCAGTTACTGAGATCAGAGCTTCAAAAGTTCATCCATAAAGATTTTCATGAAGTTGTTCAGAGGATGACTGTTATCGACACTCTGCTCTTCTTGATTGTGCATTTGATTGATAAGTTGGGAGTTTGGCATAAACTACCAGTGATTTTGGGACTAATTTATATAGCAATTCGTCGTCATATTCATCAAGAATATAACTTGTTCAATGTCGGTAGAACTCCGGTCGGAGTTCGGTTCAACTCAGTGGATTTTCCATACCGAACTGCTAATGGCAAGTACAATGATCCCTTCAATGAAGCTGTTGGTGCCCAAGGATCCTTCTTCGGTAGAAACATCCTCCCCATCGACCAAACCAAAAGGTTATTGAAACCCAACCCAATGGTGGTTGCTACAAAGCTTCTGGCAAGGAAAAAGCTTATAGACACTGGAAAACAATTCAACATGATTGCTGTTTTTTGGATTCAGTTCATGATTCATGATTGGATGGATCATTTAGAGGATACCAAGCAGATCCAATACATATATATATATATAATATTCTTCTTAATTATTTAA

Coding sequence (CDS)

ATGATTTCTCAGTTACTGAGATCAGAGCTTCAAAAGTTCATCCATAAAGATTTTCATGAAGTTGTTCAGAGGATGACTGTTATCGACACTCTGCTCTTCTTGATTGTGCATTTGATTGATAAGTTGGGAGTTTGGCATAAACTACCAGTGATTTTGGGACTAATTTATATAGCAATTCGTCGTCATATTCATCAAGAATATAACTTGTTCAATGTCGGTAGAACTCCGGTCGGAGTTCGGTTCAACTCAGTGGATTTTCCATACCGAACTGCTAATGGCAAGTACAATGATCCCTTCAATGAAGCTGTTGGTGCCCAAGGATCCTTCTTCGGTAGAAACATCCTCCCCATCGACCAAACCAAAAGGTTATTGAAACCCAACCCAATGGTGGTTGCTACAAAGCTTCTGGCAAGGAAAAAGCTTATAGACACTGGAAAACAATTCAACATGATTGCTGTTTTTTGGATTCAGTTCATGATTCATGATTGGATGGATCATTTAGAGGATACCAAGCAGATCCAATACATATATATATATATAATATTCTTCTTAATTATTTAA

Protein sequence

MISQLLRSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQYIYIYIIFFLII

Homology

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BLAST of MC08g_new0227 vs. ExPASy Swiss-Prot
Match: Q9SGH6 (Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 3.0e-67
Identity = 116/176 (65.91%), Postives = 144/176 (81.82%), Query Frame = 0


Query: 1   MISQLLRSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIR 60
           +I+ L+ S L KFIHKDFHE+  RM+++D  L LIVH +DK+  WHKLPV LGL Y+ +R
Sbjct: 3   VITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVR 62

Query: 61  RHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQT 120
           RH+HQ+YNL NVG+TP G+RF+  ++PYRTA+GK+NDPFNE VG+Q SFFGRN  P+DQ 
Sbjct: 63  RHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQK 122

Query: 121 KRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQYI 177
            +L +P+PMVVATKLL RKK IDTGKQFNMIA  WIQFMIHDW+DHLEDT QI+ +
Sbjct: 123 SKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELV 178

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BLAST of MC08g_new0227 vs. ExPASy Swiss-Prot
Match: Q9C9U3 (Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 4.8e-49
Identity = 92/163 (56.44%), Postives = 120/163 (73.62%), Query Frame = 0


Query: 13  FIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIRRHIHQEYNLFNV 72
           F+H   H VV +M+  D  LF IVHL+DKLG+WH+ PV+LG+ Y+ +RRH+HQ YNL +V
Sbjct: 10  FLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHV 69

Query: 73  GRTPV-GVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQTKRLLKPNPMVV 132
           G  P+ G  +++ +F YRTA+GK N P +  +G+QGSF GRN+ P      +L P+P VV
Sbjct: 70  G--PINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGILDPHPSVV 129

Query: 133 ATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQ 175
           ATKLLARK+ ID G QFN+IA  WIQFMIHDW+DHLEDT QI+
Sbjct: 130 ATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIE 170

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BLAST of MC08g_new0227 vs. NCBI nr
Match: XP_022150068.1 (alpha-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 324 bits (830), Expect = 9.03e-105
Identity = 152/174 (87.36%), Postives = 168/174 (96.55%), Query Frame = 0


Query: 1   MISQLLRSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIR 60
           M+SQLLRS+LQKFIH+DFHE V+RMTVIDTLLFLIVHL+DKLGVWHKLPVILGL+Y+AIR
Sbjct: 1   MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60

Query: 61  RHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQT 120
           RH+HQEYNLFNVGRTPVGVRFN VDFPYRTA+G YNDPFNEA GAQGSFFGRNILP+DQT
Sbjct: 61  RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120

Query: 121 KRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQ 174
           K+LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ++
Sbjct: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVE 174

[Close]

BLAST of MC08g_new0227 vs. NCBI nr
Match: XP_004147441.2 (alpha-dioxygenase 1 [Cucumis sativus] >KGN59637.1 hypothetical protein Csa_002419 [Cucumis sativus])

HSP 1 Score: 311 bits (798), Expect = 6.36e-100
Identity = 148/181 (81.77%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVVQRMTVIDT LFLIVH +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVQRMTVIDTFLFLIVHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VDFPYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. NCBI nr
Match: TYK13980.1 (alpha-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 309 bits (791), Expect = 8.81e-100
Identity = 145/181 (80.11%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVV+RMTVIDT LFLI+H +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VD+PYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. NCBI nr
Match: XP_008443484.1 (PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 309 bits (791), Expect = 7.08e-99
Identity = 145/181 (80.11%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVV+RMTVIDT LFLI+H +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VD+PYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. NCBI nr
Match: XP_038903383.1 (alpha-dioxygenase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 308 bits (790), Expect = 9.75e-99
Identity = 144/173 (83.24%), Postives = 159/173 (91.91%), Query Frame = 0


Query: 2   ISQLLRSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIRR 61
           I  +LR ELQKFIHKDFHEVVQRMTVIDT LFLI+H IDKLGVWHKLPVILGL+Y+A+RR
Sbjct: 9   IKAVLRGELQKFIHKDFHEVVQRMTVIDTFLFLIIHFIDKLGVWHKLPVILGLLYLAVRR 68

Query: 62  HIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQTK 121
           H+HQEYNLFNVGRTPVGVRFN +DFPYRTA+GKYNDPFNE  GAQG+FFGRNI P+DQ+K
Sbjct: 69  HLHQEYNLFNVGRTPVGVRFNPMDFPYRTADGKYNDPFNEGAGAQGTFFGRNIHPVDQSK 128

Query: 122 RLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQ 174
            LLKP+PMVVATKLL R+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+ 
Sbjct: 129 MLLKPDPMVVATKLLTRRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVD 181

[Close]

BLAST of MC08g_new0227 vs. ExPASy TrEMBL
Match: A0A6J1D8G8 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=1)

HSP 1 Score: 324 bits (830), Expect = 4.37e-105
Identity = 152/174 (87.36%), Postives = 168/174 (96.55%), Query Frame = 0


Query: 1   MISQLLRSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIR 60
           M+SQLLRS+LQKFIH+DFHE V+RMTVIDTLLFLIVHL+DKLGVWHKLPVILGL+Y+AIR
Sbjct: 1   MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60

Query: 61  RHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQT 120
           RH+HQEYNLFNVGRTPVGVRFN VDFPYRTA+G YNDPFNEA GAQGSFFGRNILP+DQT
Sbjct: 61  RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120

Query: 121 KRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQ 174
           K+LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ++
Sbjct: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVE 174

[Close]

BLAST of MC08g_new0227 vs. ExPASy TrEMBL
Match: A0A0A0LG46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829270 PE=4 SV=1)

HSP 1 Score: 311 bits (798), Expect = 3.08e-100
Identity = 148/181 (81.77%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVVQRMTVIDT LFLIVH +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVQRMTVIDTFLFLIVHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VDFPYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. ExPASy TrEMBL
Match: A0A5D3CRF7 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00040 PE=4 SV=1)

HSP 1 Score: 309 bits (791), Expect = 4.27e-100
Identity = 145/181 (80.11%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVV+RMTVIDT LFLI+H +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VD+PYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. ExPASy TrEMBL
Match: A0A5A7UJ02 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00690 PE=4 SV=1)

HSP 1 Score: 309 bits (791), Expect = 3.43e-99
Identity = 145/181 (80.11%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVV+RMTVIDT LFLI+H +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VD+PYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. ExPASy TrEMBL
Match: A0A1S3B8X6 (alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1)

HSP 1 Score: 309 bits (791), Expect = 3.43e-99
Identity = 145/181 (80.11%), Postives = 164/181 (90.61%), Query Frame = 0


Query: 1   MISQLL-------RSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILG 60
           M+SQLL       R ELQKF+HKDFHEVV+RMTVIDT LFLI+H +DKLG+WHKLPVILG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LIYIAIRRHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRN 120
           L+Y+A+RRH+HQEYNLFNVGRTPVGVRFN VD+PYRTA+GKYNDPFNE  GAQGSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPIDQTKRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQI 174
           I P+DQ+K LLKP+PMVVATKLLAR+KLIDTGKQFNMIA  WIQFMIHDW+DHLEDTKQ+
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

[Close]

BLAST of MC08g_new0227 vs. TAIR 10
Match: AT3G01420.1 (Peroxidase superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 2.1e-68
Identity = 116/176 (65.91%), Postives = 144/176 (81.82%), Query Frame = 0


Query: 1   MISQLLRSELQKFIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIR 60
           +I+ L+ S L KFIHKDFHE+  RM+++D  L LIVH +DK+  WHKLPV LGL Y+ +R
Sbjct: 3   VITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVR 62

Query: 61  RHIHQEYNLFNVGRTPVGVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQT 120
           RH+HQ+YNL NVG+TP G+RF+  ++PYRTA+GK+NDPFNE VG+Q SFFGRN  P+DQ 
Sbjct: 63  RHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQK 122

Query: 121 KRLLKPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQYI 177
            +L +P+PMVVATKLL RKK IDTGKQFNMIA  WIQFMIHDW+DHLEDT QI+ +
Sbjct: 123 SKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELV 178

[Close]

BLAST of MC08g_new0227 vs. TAIR 10
Match: AT1G73680.1 (alpha dioxygenase )

HSP 1 Score: 195.7 bits (496), Expect = 3.4e-50
Identity = 92/163 (56.44%), Postives = 120/163 (73.62%), Query Frame = 0


Query: 13  FIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIRRHIHQEYNLFNV 72
           F+H   H VV +M+  D  LF IVHL+DKLG+WH+ PV+LG+ Y+ +RRH+HQ YNL +V
Sbjct: 10  FLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHV 69

Query: 73  GRTPV-GVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQTKRLLKPNPMVV 132
           G  P+ G  +++ +F YRTA+GK N P +  +G+QGSF GRN+ P      +L P+P VV
Sbjct: 70  G--PINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGILDPHPSVV 129

Query: 133 ATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQ 175
           ATKLLARK+ ID G QFN+IA  WIQFMIHDW+DHLEDT QI+
Sbjct: 130 ATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIE 170

[Close]

BLAST of MC08g_new0227 vs. TAIR 10
Match: AT1G73680.2 (alpha dioxygenase )

HSP 1 Score: 179.5 bits (454), Expect = 2.5e-45
Identity = 89/172 (51.74%), Postives = 116/172 (67.44%), Query Frame = 0


Query: 13  FIHKDFHEVVQRMTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIRRHIHQEYNLFNV 72
           F+H   H VV +M+  D  LF IVHL+DKLG+WH+ PV+LG+ Y+ +RRH+HQ YNL +V
Sbjct: 10  FLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHV 69

Query: 73  GRTPV-GVRFNSVDFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQTKRLL------- 132
           G  P+ G  +++ +F YRTA+GK N P +  +G+QGSF GRN+ P               
Sbjct: 70  G--PINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGFFCQYFFFP 129

Query: 133 --KPNPMVVATKLLARKKLIDTGKQFNMIAVFWIQFMIHDWMDHLEDTKQIQ 175
                 +VVATKLLARK+ ID G QFN+IA  WIQFMIHDW+DHLEDT QI+
Sbjct: 130 ENLEKKIVVATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIE 179

The following BLAST results are available for this feature:

BLAST of MC08g_new0227 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGH6	3.0e-67	65.91	Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1	[more]
Q9C9U3	4.8e-49	56.44	Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1	[more]

BLAST of MC08g_new0227 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150068.1	9.03e-105	87.36	alpha-dioxygenase 1-like [Momordica charantia]	[more]
XP_004147441.2	6.36e-100	81.77	alpha-dioxygenase 1 [Cucumis sativus] >KGN59637.1 hypothetical protein Csa_00241...	[more]
TYK13980.1	8.81e-100	80.11	alpha-dioxygenase 1 [Cucumis melo var. makuwa]	[more]
XP_008443484.1	7.08e-99	80.11	PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 ...	[more]
XP_038903383.1	9.75e-99	83.24	alpha-dioxygenase 1-like isoform X2 [Benincasa hispida]	[more]

BLAST of MC08g_new0227 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8G8	4.37e-105	87.36	alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=...	[more]
A0A0A0LG46	3.08e-100	81.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829270 PE=4 SV=1	[more]
A0A5D3CRF7	4.27e-100	80.11	Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119...	[more]
A0A5A7UJ02	3.43e-99	80.11	Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G...	[more]
A0A1S3B8X6	3.43e-99	80.11	alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1	[more]

BLAST of MC08g_new0227 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01420.1	2.1e-68	65.91	Peroxidase superfamily protein	[more]
AT1G73680.1	3.4e-50	56.44	alpha dioxygenase	[more]
AT1G73680.2	2.5e-45	51.74	alpha dioxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037120	Haem peroxidase domain superfamily, animal type	GENE3D	1.10.640.10	Haem peroxidase domain superfamily, animal type	coord: 4..179 e-value: 6.1E-40 score: 139.1
IPR019791	Haem peroxidase, animal-type	PFAM	PF03098	An_peroxidase	coord: 88..171 e-value: 6.8E-16 score: 57.9
IPR019791	Haem peroxidase, animal-type	PROSITE	PS50292	PEROXIDASE_3	coord: 78..186 score: 15.162342
None	No IPR available	PANTHER	PTHR11903	PROSTAGLANDIN G/H SYNTHASE	coord: 6..174
None	No IPR available	PANTHER	PTHR11903:SF27	ALPHA-DIOXYGENASE 1-LIKE	coord: 6..174
IPR010255	Haem peroxidase superfamily	SUPERFAMILY	48113	Heme-dependent peroxidases	coord: 84..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0227.1	MC08g_new0227.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098869	cellular oxidant detoxification
biological_process	GO:0006979	response to oxidative stress
molecular_function	GO:0020037	heme binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function	GO:0004601	peroxidase activity