MC08g_new0066 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0066
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	17.9 kDa class II heat shock protein-like
Location	MC08: 3981982 .. 3983079 (+)
RNA-Seq Expression	MC08g_new0066
Synteny	MC08g_new0066

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCCATTATTCAATCTCCTGTGCGACAGTCACATTTATAATTCTATATTTGAAAAATATATTCCAAATATATGCTAGTTTAGTTTTAAATTTTAATTCACACCGCTGAAATAAAAGGAATTGGACTTTCTAAAAAAAAAAAAAAAAGCTTTCATATTCTAAAACATTCGAGACCCAATAGAAGAGAAGGGGACCCACTTAGCATCTAGAACCATCGAAACCCTTTACTTTCTTCTTTCAGAACCTTCTTGAACTTTCCGGCGACCGCCGATTCCACCGCCCTCCACGCATATTAATACCATCTCTCCCGGAATTCCACATCCATCTTCTAATTTCAATTCACCGGAGCTTTCTCTGTAATTTCAAGAACAAAGAGAGATCGTTTTCCCGCGAGCGACGCCGATGGATTTCAGAATTCTGGATTCTCCTCTGTTCTCCACCCTGCAACAAATGGTGGATATGGCCGACGGCGGCGCCGGCAAGCCCATCACCGGCCCGACCCGTGCCTACGTGCGGGACGCGACGGCCATGGCCGCCACTCCGGTGGACATAATCGAATACCCGAATTCGTACGTTTTCGCGATCGACATGCCGGGAGCGAAATCAGGGGAAATTAGGGTTCAGGTGGAGGACGAGAACGTGCTTCTGATCACCGGCGAGAGGAAGAGAGAGGAGGAGAAAGAATCGGCGAAGTATTTGAGAATGGAGCGGAGGATCGGAAAATTCATGAGGAAATTTATGTTGCCGGAGAATGCGGATACGAACGCCGTGACGGCGGTGTGCCACGACGGCGTTCTCACGGTGACTGTGCAGAAGCTGCCGCCGCCGGAGCCGAAGAAGCCGAAGACGATTGAGGTTCAGATAGCTTAATTTAATCCATGGCTTTTGGAAGATTTCTGTGATGTGGGAAAATTGTAATGTTAGAACAAATAAAGTCTCTGTTCTGTGAGAATTCAACCAAAAAAAAGAAAAAAAAAATCTGTTGTGTGAAATTTGATCTTTCAATCTCAATTTCCATCCATCCTATGCTTCTTCCTAAATCCTCTTTTAACTGGAAATAATACGTTCTTCAAAAACACTGTTTTAGTTAATGGCCGAA

mRNA sequence

ATGGATTTCAGAATTCTGGATTCTCCTCTGTTCTCCACCCTGCAACAAATGGTGGATATGGCCGACGGCGGCGCCGGCAAGCCCATCACCGGCCCGACCCGTGCCTACGTGCGGGACGCGACGGCCATGGCCGCCACTCCGGTGGACATAATCGAATACCCGAATTCGTACGTTTTCGCGATCGACATGCCGGGAGCGAAATCAGGGGAAATTAGGGTTCAGGTGGAGGACGAGAACGTGCTTCTGATCACCGGCGAGAGGAAGAGAGAGGAGGAGAAAGAATCGGCGAAGTATTTGAGAATGGAGCGGAGGATCGGAAAATTCATGAGGAAATTTATGTTGCCGGAGAATGCGGATACGAACGCCGTGACGGCGGTGTGCCACGACGGCGTTCTCACGGTGACTGTGCAGAAGCTGCCGCCGCCGGAGCCGAAGAAGCCGAAGACGATTGAGGTTCAGATAGCTTAA

Coding sequence (CDS)

ATGGATTTCAGAATTCTGGATTCTCCTCTGTTCTCCACCCTGCAACAAATGGTGGATATGGCCGACGGCGGCGCCGGCAAGCCCATCACCGGCCCGACCCGTGCCTACGTGCGGGACGCGACGGCCATGGCCGCCACTCCGGTGGACATAATCGAATACCCGAATTCGTACGTTTTCGCGATCGACATGCCGGGAGCGAAATCAGGGGAAATTAGGGTTCAGGTGGAGGACGAGAACGTGCTTCTGATCACCGGCGAGAGGAAGAGAGAGGAGGAGAAAGAATCGGCGAAGTATTTGAGAATGGAGCGGAGGATCGGAAAATTCATGAGGAAATTTATGTTGCCGGAGAATGCGGATACGAACGCCGTGACGGCGGTGTGCCACGACGGCGTTCTCACGGTGACTGTGCAGAAGCTGCCGCCGCCGGAGCCGAAGAAGCCGAAGACGATTGAGGTTCAGATAGCTTAA

Protein sequence

MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA

Homology

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BLAST of MC08g_new0066 vs. ExPASy Swiss-Prot
Match: P05477 (17.9 kDa class II heat shock protein OS=Glycine max OX=3847 GN=HSP17.9-D PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 3.2e-59
Identity = 115/159 (72.33%), Postives = 138/159 (86.79%), Query Frame = 0


Query: 1   MDFRI--LDSPLFSTLQQMVDMADGGAG--KPITGPTRAYVRDATAMAATPVDIIEYPNS 60
           MDFR+  L+SPLF TLQ M+DM++ GAG  K    PT +YVRDA AMAATP D+ EYPNS
Sbjct: 1   MDFRVMGLESPLFHTLQHMMDMSEDGAGDNKTHNAPTWSYVRDAKAMAATPADVKEYPNS 60

Query: 61  YVFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPE 120
           YVF IDMPG KSG+I+VQVED+N+LLI GERKR+EEKE AKYLRMERR+GK MRKF+LPE
Sbjct: 61  YVFEIDMPGLKSGDIKVQVEDDNLLLICGERKRDEEKEGAKYLRMERRVGKLMRKFVLPE 120

Query: 121 NADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           NA+T+A++AVC DGVL+VTVQKLPPPEPKKP+TI+V++A
Sbjct: 121 NANTDAISAVCQDGVLSVTVQKLPPPEPKKPRTIQVKVA 159

[Close]

BLAST of MC08g_new0066 vs. ExPASy Swiss-Prot
Match: P19242 (17.1 kDa class II heat shock protein OS=Pisum sativum OX=3888 GN=HSP17.7 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.3e-56
Identity = 106/150 (70.67%), Postives = 126/150 (84.00%), Query Frame = 0


Query: 6   LDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFAIDMPG 65
           LDSPLF+TL  ++D+ D    K +  PTR YVRDA AMAATP D+ E+PNSYVF +DMPG
Sbjct: 3   LDSPLFNTLHHIMDLTDDTTEKNLNAPTRTYVRDAKAMAATPADVKEHPNSYVFMVDMPG 62

Query: 66  AKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADTNAVTA 125
            KSG+I+VQVEDENVLLI+GERKREEEKE  KYL+MERRIGK MRKF+LPENA+  A++A
Sbjct: 63  VKSGDIKVQVEDENVLLISGERKREEEKEGVKYLKMERRIGKLMRKFVLPENANIEAISA 122

Query: 126 VCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           +  DGVLTVTV KLPPPEPKKPKTI+V++A
Sbjct: 123 ISQDGVLTVTVNKLPPPEPKKPKTIQVKVA 152

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BLAST of MC08g_new0066 vs. ExPASy Swiss-Prot
Match: O82013 (17.3 kDa class II heat shock protein OS=Solanum peruvianum OX=4082 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.7e-55
Identity = 108/158 (68.35%), Postives = 133/158 (84.18%), Query Frame = 0


Query: 1   MDFRIL---DSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSY 60
           MD R+L   ++PLF TL  M++ A   +   +  P++ YVRDA AMAATP D+ EYPNSY
Sbjct: 1   MDLRLLGIDNTPLFHTLHHMMEAAGEDS---VNAPSKIYVRDAKAMAATPADVKEYPNSY 60

Query: 61  VFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPEN 120
           VF +DMPG KSG+I+VQVE++NVLLI+GERKREEEKE AK++RMERR+GKFMRKF LPEN
Sbjct: 61  VFVVDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKFIRMERRVGKFMRKFSLPEN 120

Query: 121 ADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           A+T+A++AVC DGVLTVTVQKLPPPEPKKPKTIEV++A
Sbjct: 121 ANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKVA 155

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BLAST of MC08g_new0066 vs. ExPASy Swiss-Prot
Match: P24632 (17.8 kDa class II heat shock protein OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-50
Identity = 100/157 (63.69%), Postives = 125/157 (79.62%), Query Frame = 0


Query: 6   LDSPLFSTLQQMVDMADGGAG-----KPITG--PTRAYVRDATAMAATPVDIIEYPNSYV 65
           L++PL + LQ ++D+ DG AG     K  +G   TR YVRDA AMAATP D+ E P +Y 
Sbjct: 8   LETPLMAALQHLLDVPDGDAGAGGDNKTGSGGSATRTYVRDARAMAATPADVKELPGAYA 67

Query: 66  FAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENA 125
           F +DMPG  +G+IRVQVEDE VL+++GER+REE ++ AKYLRMERR+GKFMRKF+LP+NA
Sbjct: 68  FVVDMPGLGTGDIRVQVEDERVLVVSGERRREEREDDAKYLRMERRMGKFMRKFVLPDNA 127

Query: 126 DTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           D + V AVC DGVLTVTV+KLPPPEPKKPKTIEV++A
Sbjct: 128 DVDKVAAVCRDGVLTVTVEKLPPPEPKKPKTIEVKVA 164

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BLAST of MC08g_new0066 vs. ExPASy Swiss-Prot
Match: Q5VRY1 (18.0 kDa class II heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP18.0 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-49
Identity = 100/159 (62.89%), Postives = 128/159 (80.50%), Query Frame = 0


Query: 6   LDSPLFSTLQQMVDMAD---GGAGK--PITGPTRAYVRDATAMAATPVDIIEYPNSYVFA 65
           L++PL + LQ ++D+ D   G AGK     GPTRAYVRDA AMAATP D+ + P +Y F 
Sbjct: 8   LETPLMTALQHLLDIPDGEGGAAGKQGATGGPTRAYVRDARAMAATPADVKDLPGAYAFV 67

Query: 66  IDMPGAKSGEIRVQVEDENVLLITGERKR----EEEKESAKYLRMERRIGKFMRKFMLPE 125
           +DMPG KS +I+VQVE+E +L+I+GER+R    EE++ES KYLRMERR+GKFMRKF+LP+
Sbjct: 68  VDMPGLKSSDIKVQVEEERLLVISGERRRGGGEEEKEESCKYLRMERRMGKFMRKFVLPD 127

Query: 126 NADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           NAD + ++AVC DGVLTVTV+KLPPPEPKKPKTIEV++A
Sbjct: 128 NADVDKISAVCQDGVLTVTVEKLPPPEPKKPKTIEVKVA 166

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BLAST of MC08g_new0066 vs. NCBI nr
Match: XP_022131987.1 (17.9 kDa class II heat shock protein-like [Momordica charantia])

HSP 1 Score: 300 bits (769), Expect = 1.32e-102
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFA 60
           MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVD IEYPNSYVFA
Sbjct: 1   MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDXIEYPNSYVFA 60

Query: 61  IDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADT 120
           IDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADT
Sbjct: 61  IDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADT 120

Query: 121 NAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           NAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA
Sbjct: 121 NAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155

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BLAST of MC08g_new0066 vs. NCBI nr
Match: XP_030501888.1 (17.9 kDa class II heat shock protein [Cannabis sativa] >KAF4351361.1 hypothetical protein F8388_022736 [Cannabis sativa] >KAF4399184.1 hypothetical protein G4B88_023778 [Cannabis sativa])

HSP 1 Score: 238 bits (608), Expect = 5.29e-78
Identity = 123/159 (77.36%), Postives = 139/159 (87.42%), Query Frame = 0


Query: 1   MDFRIL--DSPLFSTLQQMVDMADGGA--GKPITGPTRAYVRDATAMAATPVDIIEYPNS 60
           MDFRIL  DSPLF+TL QM+D ++  A   K I  PTR YVRDA AMAATP D+ EYPNS
Sbjct: 1   MDFRILGLDSPLFTTLNQMIDNSEDSADHSKSINAPTRTYVRDAKAMAATPADVKEYPNS 60

Query: 61  YVFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPE 120
           YVF IDMPG KSG+I+VQVED+NVLLI+GERKREEEKE AKY+RMERR+GKFMRKF+LPE
Sbjct: 61  YVFIIDMPGLKSGDIKVQVEDDNVLLISGERKREEEKEGAKYVRMERRVGKFMRKFVLPE 120

Query: 121 NADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           NADT+A++AVC DGVLTVTVQKLPPPEPKKPKTIEV+IA
Sbjct: 121 NADTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKIA 159

[Close]

BLAST of MC08g_new0066 vs. NCBI nr
Match: XP_031259376.1 (17.9 kDa class II heat shock protein-like [Pistacia vera])

HSP 1 Score: 236 bits (603), Expect = 2.86e-77
Identity = 118/157 (75.16%), Postives = 138/157 (87.90%), Query Frame = 0


Query: 1   MDFRI--LDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYV 60
           MD RI  LDSPLFSTLQ M+D+A+  + K    PTR YVRDA AMAATP D+ EYPNSYV
Sbjct: 1   MDLRIMGLDSPLFSTLQHMMDVAEHDSDKSFNAPTRTYVRDAKAMAATPADVKEYPNSYV 60

Query: 61  FAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENA 120
           F IDMPG KSG+I+VQVED+NVLLI+GERKR+EEKE AKY+RMERR+GKFMRKF+LPENA
Sbjct: 61  FIIDMPGLKSGDIKVQVEDDNVLLISGERKRDEEKEGAKYVRMERRVGKFMRKFVLPENA 120

Query: 121 DTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           +TNA++A+C DGVLTVTV+K+PPPEPKKPKTIEV+IA
Sbjct: 121 NTNAISAICQDGVLTVTVEKMPPPEPKKPKTIEVKIA 157

[Close]

BLAST of MC08g_new0066 vs. NCBI nr
Match: PON95972.1 (Small heat shock protein HSP [Trema orientale])

HSP 1 Score: 234 bits (597), Expect = 2.42e-76
Identity = 120/158 (75.95%), Postives = 138/158 (87.34%), Query Frame = 0


Query: 1   MDFRI--LDSPLFSTLQQMVDMADGGAG-KPITGPTRAYVRDATAMAATPVDIIEYPNSY 60
           MDFRI  LDSPLFSTL  M+D +D  A  +    PTR YVRDA AMA+TP D++EYPNSY
Sbjct: 1   MDFRIVGLDSPLFSTLHHMIDASDDAADHRSFNAPTRTYVRDAKAMASTPADVMEYPNSY 60

Query: 61  VFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPEN 120
           VF IDMPG KSG+I+VQVE++NVLLI+GERKREEEKE AKY+RMERRIGKFMRKF+LPEN
Sbjct: 61  VFIIDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKYVRMERRIGKFMRKFVLPEN 120

Query: 121 ADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           A+T+A++AVC DGVLTVTVQKLPPPEPKKPKTIEV+IA
Sbjct: 121 ANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKIA 158

[Close]

BLAST of MC08g_new0066 vs. NCBI nr
Match: AIM18898.1 (heat shock protein 17.9 [Prosopis cineraria])

HSP 1 Score: 233 bits (593), Expect = 9.53e-76
Identity = 116/153 (75.82%), Postives = 130/153 (84.97%), Query Frame = 0


Query: 3   FRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFAID 62
           F  LDSPLF  LQ M+D+ D  A K +  P+R Y RDA AMAATP DI EYPNSYVF ID
Sbjct: 5   FSALDSPLFHALQHMMDVTDDAADKSVNAPSRTYARDAKAMAATPADIKEYPNSYVFIID 64

Query: 63  MPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADTNA 122
           MPG KSG+I+VQVED+NVL ITGERKREEEKE AKY+RMERR+GKFMRKF+LPENA+T+A
Sbjct: 65  MPGLKSGDIKVQVEDDNVLQITGERKREEEKEGAKYVRMERRVGKFMRKFVLPENANTDA 124

Query: 123 VTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           ++AVC DGVLTVTVQKLPPPEPKKPKTIEV IA
Sbjct: 125 ISAVCQDGVLTVTVQKLPPPEPKKPKTIEVMIA 157

[Close]

BLAST of MC08g_new0066 vs. ExPASy TrEMBL
Match: A0A6J1BSL2 (17.9 kDa class II heat shock protein-like OS=Momordica charantia OX=3673 GN=LOC111004967 PE=3 SV=1)

HSP 1 Score: 300 bits (769), Expect = 6.41e-103
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFA 60
           MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVD IEYPNSYVFA
Sbjct: 1   MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDXIEYPNSYVFA 60

Query: 61  IDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADT 120
           IDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADT
Sbjct: 61  IDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADT 120

Query: 121 NAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           NAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA
Sbjct: 121 NAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155

[Close]

BLAST of MC08g_new0066 vs. ExPASy TrEMBL
Match: A0A7J6HV41 (SHSP domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_022736 PE=3 SV=1)

HSP 1 Score: 238 bits (608), Expect = 2.56e-78
Identity = 123/159 (77.36%), Postives = 139/159 (87.42%), Query Frame = 0


Query: 1   MDFRIL--DSPLFSTLQQMVDMADGGA--GKPITGPTRAYVRDATAMAATPVDIIEYPNS 60
           MDFRIL  DSPLF+TL QM+D ++  A   K I  PTR YVRDA AMAATP D+ EYPNS
Sbjct: 1   MDFRILGLDSPLFTTLNQMIDNSEDSADHSKSINAPTRTYVRDAKAMAATPADVKEYPNS 60

Query: 61  YVFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPE 120
           YVF IDMPG KSG+I+VQVED+NVLLI+GERKREEEKE AKY+RMERR+GKFMRKF+LPE
Sbjct: 61  YVFIIDMPGLKSGDIKVQVEDDNVLLISGERKREEEKEGAKYVRMERRVGKFMRKFVLPE 120

Query: 121 NADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           NADT+A++AVC DGVLTVTVQKLPPPEPKKPKTIEV+IA
Sbjct: 121 NADTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKIA 159

[Close]

BLAST of MC08g_new0066 vs. ExPASy TrEMBL
Match: A0A803PPZ7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 238 bits (608), Expect = 2.56e-78
Identity = 123/159 (77.36%), Postives = 139/159 (87.42%), Query Frame = 0


Query: 1   MDFRIL--DSPLFSTLQQMVDMADGGA--GKPITGPTRAYVRDATAMAATPVDIIEYPNS 60
           MDFRIL  DSPLF+TL QM+D ++  A   K I  PTR YVRDA AMAATP D+ EYPNS
Sbjct: 1   MDFRILGLDSPLFTTLNQMIDNSEDSADHSKSINAPTRTYVRDAKAMAATPADVKEYPNS 60

Query: 61  YVFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPE 120
           YVF IDMPG KSG+I+VQVED+NVLLI+GERKREEEKE AKY+RMERR+GKFMRKF+LPE
Sbjct: 61  YVFIIDMPGLKSGDIKVQVEDDNVLLISGERKREEEKEGAKYVRMERRVGKFMRKFVLPE 120

Query: 121 NADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           NADT+A++AVC DGVLTVTVQKLPPPEPKKPKTIEV+IA
Sbjct: 121 NADTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKIA 159

[Close]

BLAST of MC08g_new0066 vs. ExPASy TrEMBL
Match: A0A2P5FDV9 (Small heat shock protein HSP OS=Trema orientale OX=63057 GN=TorRG33x02_082200 PE=3 SV=1)

HSP 1 Score: 234 bits (597), Expect = 1.17e-76
Identity = 120/158 (75.95%), Postives = 138/158 (87.34%), Query Frame = 0


Query: 1   MDFRI--LDSPLFSTLQQMVDMADGGAG-KPITGPTRAYVRDATAMAATPVDIIEYPNSY 60
           MDFRI  LDSPLFSTL  M+D +D  A  +    PTR YVRDA AMA+TP D++EYPNSY
Sbjct: 1   MDFRIVGLDSPLFSTLHHMIDASDDAADHRSFNAPTRTYVRDAKAMASTPADVMEYPNSY 60

Query: 61  VFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPEN 120
           VF IDMPG KSG+I+VQVE++NVLLI+GERKREEEKE AKY+RMERRIGKFMRKF+LPEN
Sbjct: 61  VFIIDMPGLKSGDIKVQVEEDNVLLISGERKREEEKEGAKYVRMERRIGKFMRKFVLPEN 120

Query: 121 ADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           A+T+A++AVC DGVLTVTVQKLPPPEPKKPKTIEV+IA
Sbjct: 121 ANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVKIA 158

[Close]

BLAST of MC08g_new0066 vs. ExPASy TrEMBL
Match: A0A2I4JFP8 (Heat shock protein 17.9 OS=Prosopis cineraria OX=364024 GN=HSP 17.9 PE=2 SV=1)

HSP 1 Score: 233 bits (593), Expect = 4.62e-76
Identity = 116/153 (75.82%), Postives = 130/153 (84.97%), Query Frame = 0


Query: 3   FRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFAID 62
           F  LDSPLF  LQ M+D+ D  A K +  P+R Y RDA AMAATP DI EYPNSYVF ID
Sbjct: 5   FSALDSPLFHALQHMMDVTDDAADKSVNAPSRTYARDAKAMAATPADIKEYPNSYVFIID 64

Query: 63  MPGAKSGEIRVQVEDENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADTNA 122
           MPG KSG+I+VQVED+NVL ITGERKREEEKE AKY+RMERR+GKFMRKF+LPENA+T+A
Sbjct: 65  MPGLKSGDIKVQVEDDNVLQITGERKREEEKEGAKYVRMERRVGKFMRKFVLPENANTDA 124

Query: 123 VTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 155
           ++AVC DGVLTVTVQKLPPPEPKKPKTIEV IA
Sbjct: 125 ISAVCQDGVLTVTVQKLPPPEPKKPKTIEVMIA 157

[Close]

BLAST of MC08g_new0066 vs. TAIR 10
Match: AT5G12020.1 (17.6 kDa class II heat shock protein )

HSP 1 Score: 192.2 bits (487), Expect = 3.1e-49
Identity = 94/149 (63.09%), Postives = 120/149 (80.54%), Query Frame = 0


Query: 9   PLFSTLQQMVDM-ADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFAIDMPGAK 68
           P+ S L+ M+++  D    K    P+R Y+RDA AMAATP D+IE+PN+Y F +DMPG K
Sbjct: 7   PIISILEDMLEVPEDHNNEKTRNNPSRVYMRDAKAMAATPADVIEHPNAYAFVVDMPGIK 66

Query: 69  SGEIRVQVEDENVLLITGERKRE-EEKESAKYLRMERRIGKFMRKFMLPENADTNAVTAV 128
             EI+VQVE++NVL+++GER+RE +E E  KY+RMERR+GKFMRKF LPENAD + ++AV
Sbjct: 67  GDEIKVQVENDNVLVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLPENADLDKISAV 126

Query: 129 CHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           CHDGVL VTVQKLPPPEPKKPKTI+VQ+A
Sbjct: 127 CHDGVLKVTVQKLPPPEPKKPKTIQVQVA 155

[Close]

BLAST of MC08g_new0066 vs. TAIR 10
Match: AT5G12030.1 (heat shock protein 17.6A )

HSP 1 Score: 188.3 bits (477), Expect = 4.5e-48
Identity = 90/156 (57.69%), Postives = 122/156 (78.21%), Query Frame = 0


Query: 1   MDFRILDSPLFSTLQQMVDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFA 60
           MD      P+FS L+ M++  +    K    P+RAY+RDA AMAATP D+IE+P++YVFA
Sbjct: 1   MDLEFGRFPIFSILEDMLEAPEEQTEKTRNNPSRAYMRDAKAMAATPADVIEHPDAYVFA 60

Query: 61  IDMPGAKSGEIRVQVEDENVLLITGERKRE-EEKESAKYLRMERRIGKFMRKFMLPENAD 120
           +DMPG K  EI+VQ+E+ENVL+++G+R+R+ +E E  K++RMERR+GKFMRKF LP+NAD
Sbjct: 61  VDMPGIKGDEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNAD 120

Query: 121 TNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
              ++A C+DGVL VT+ KLPPPEPKKPKTI+VQ+A
Sbjct: 121 LEKISAACNDGVLKVTIPKLPPPEPKKPKTIQVQVA 156

[Close]

BLAST of MC08g_new0066 vs. TAIR 10
Match: AT1G54050.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-22
Identity = 53/114 (46.49%), Postives = 83/114 (72.81%), Query Frame = 0


Query: 47  PVDIIEYPNSYVFAIDMPGAKSGEIRVQVEDENVLLI--TGERKR--EEEKESAKYLRME 106
           P+DI+E P  Y+F +D+PG    +I+V VE+E  L+I   G+RKR  +E +E +KY+R+E
Sbjct: 43  PIDILESPKEYIFYLDIPGISKSDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRLE 102

Query: 107 RRIGK-FMRKFMLPENADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEVQIA 156
           RR+ +  ++KF LPE+AD  +VTA   +GVLTV ++KL PP+P KPKT+++ ++
Sbjct: 103 RRLAQNLVKKFRLPEDADMASVTAKYQEGVLTVVIKKL-PPQPPKPKTVQIAVS 155

[Close]

BLAST of MC08g_new0066 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 101.7 bits (252), Expect = 5.5e-22
Identity = 55/115 (47.83%), Postives = 76/115 (66.09%), Query Frame = 0


Query: 38  RDATAMAATPVDIIEYPNSYVFAIDMPGAKSGEIRVQVEDENVLLITGERKREEEKESAK 97
           RD  A     VD  E P ++VF  D+PG K  E++V+VED+NVL I+GER +E E+++ K
Sbjct: 44  RDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDK 103

Query: 98  YLRMERRIGKFMRKFMLPENADTNAVTAVCHDGVLTVTVQKLPPPEPKKPKTIEV 153
           + R+ER  GKFMR+F LPENA    V A   +GVLTV V K P  +P + K+I++
Sbjct: 104 WHRVERASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAPEKKP-QVKSIDI 157

[Close]

BLAST of MC08g_new0066 vs. TAIR 10
Match: AT3G46230.1 (heat shock protein 17.4 )

HSP 1 Score: 99.0 bits (245), Expect = 3.6e-21
Identity = 56/128 (43.75%), Postives = 77/128 (60.16%), Query Frame = 0


Query: 18  VDMADGGAGKPITGPTRAYVRDATAMAATPVDIIEYPNSYVFAIDMPGAKSGEIRVQVED 77
           +D+ D   G    G T A  +D  A     VD  E P ++VF  D+PG K  E++V+VED
Sbjct: 21  LDVWDPFEGFLTPGLTNAPAKDVAAFTNAKVDWRETPEAHVFKADVPGLKKEEVKVEVED 80

Query: 78  ENVLLITGERKREEEKESAKYLRMERRIGKFMRKFMLPENADTNAVTAVCHDGVLTVTVQ 137
            N+L I+GER  E E++S  + R+ER  GKFMR+F LPENA    V A   +GVL+VTV 
Sbjct: 81  GNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVEEVKASMENGVLSVTVP 140

Query: 138 KLPPPEPK 146
           K+   +P+
Sbjct: 141 KVQESKPE 148

The following BLAST results are available for this feature:

BLAST of MC08g_new0066 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05477	3.2e-59	72.33	17.9 kDa class II heat shock protein OS=Glycine max OX=3847 GN=HSP17.9-D PE=3 SV...	[more]
P19242	3.3e-56	70.67	17.1 kDa class II heat shock protein OS=Pisum sativum OX=3888 GN=HSP17.7 PE=1 SV...	[more]
O82013	1.7e-55	68.35	17.3 kDa class II heat shock protein OS=Solanum peruvianum OX=4082 PE=2 SV=1	[more]
P24632	3.6e-50	63.69	17.8 kDa class II heat shock protein OS=Zea mays OX=4577 PE=2 SV=1	[more]
Q5VRY1	1.0e-49	62.89	18.0 kDa class II heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]

BLAST of MC08g_new0066 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131987.1	1.32e-102	99.35	17.9 kDa class II heat shock protein-like [Momordica charantia]	[more]
XP_030501888.1	5.29e-78	77.36	17.9 kDa class II heat shock protein [Cannabis sativa] >KAF4351361.1 hypothetica...	[more]
XP_031259376.1	2.86e-77	75.16	17.9 kDa class II heat shock protein-like [Pistacia vera]	[more]
PON95972.1	2.42e-76	75.95	Small heat shock protein HSP [Trema orientale]	[more]
AIM18898.1	9.53e-76	75.82	heat shock protein 17.9 [Prosopis cineraria]	[more]

BLAST of MC08g_new0066 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSL2	6.41e-103	99.35	17.9 kDa class II heat shock protein-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A7J6HV41	2.56e-78	77.36	SHSP domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_022736 PE=3 S...	[more]
A0A803PPZ7	2.56e-78	77.36	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A2P5FDV9	1.17e-76	75.95	Small heat shock protein HSP OS=Trema orientale OX=63057 GN=TorRG33x02_082200 PE...	[more]
A0A2I4JFP8	4.62e-76	75.82	Heat shock protein 17.9 OS=Prosopis cineraria OX=364024 GN=HSP 17.9 PE=2 SV=1	[more]

BLAST of MC08g_new0066 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G12020.1	3.1e-49	63.09	17.6 kDa class II heat shock protein	[more]
AT5G12030.1	4.5e-48	57.69	heat shock protein 17.6A	[more]
AT1G54050.1	1.1e-22	46.49	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	5.5e-22	47.83	heat shock protein 18.2	[more]
AT3G46230.1	3.6e-21	43.75	heat shock protein 17.4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 49..153 e-value: 2.1E-30 score: 104.9
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 39..155 score: 28.962622
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 3..154 e-value: 4.3E-38 score: 132.4
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 32..147
None	No IPR available	PANTHER	PTHR11527:SF277	17.6 KDA CLASS II HEAT SHOCK PROTEIN-RELATED	coord: 6..155
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 6..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0066.1	MC08g_new0066.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding