MC08g_new0049 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0049
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Plant protein of unknown function (DUF247)
Location	MC08: 3152006 .. 3152529 (-)
RNA-Seq Expression	MC08g_new0049
Synteny	MC08g_new0049

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCTTTGCTTTGGCTGCTAACAAGGGTGACGCCTTAAGGCATCAACAGGACTATTGTAAAGCTTTCTATGAGACGCCCTGGCCAACTAAGTACTCTGTGGATTTGGTGGCTGATCAGCAATCGGAAGAGGATGATCTGAGTCTTGAGTTATCCATCATAAAACTTCTGGAGGAACAGCCTCCCATCGCCGCTGCAGAATGGTGCATCTACAGGGTTCCCAAACGGCTATTCGACATGAAATCTACTGCTTATACGCCTAAAGTCATTGCAATCGGCCCTTTTCACCATGGTCGAAACGATTTGCTGGCTACACAGCAGTCTAAACTCCATTGTTTCCGTAATTATCTAATCCGAATAGAACGGGATGTTAAGTACGTGGTTGCAATTGCTCGACAATGGGAGATAAAAGCCCGAAGGCTCTATGCAGAACCCATAAACATGACCAGCGATGACTTTGTCAGAATGATGCTCATAGACGCCTGTTTCATAGTGGAGCTTATGATAATATTGAATTTTGA

mRNA sequence

ATGGAGTGCTTTGCTTTGGCTGCTAACAAGGGTGACGCCTTAAGGCATCAACAGGACTATTGTAAAGCTTTCTATGAGACGCCCTGGCCAACTAAGTACTCTGTGGATTTGGTGGCTGATCAGCAATCGGAAGAGGATGATCTGAGTCTTGAGTTATCCATCATAAAACTTCTGGAGGAACAGCCTCCCATCGCCGCTGCAGAATGGTGCATCTACAGGGTTCCCAAACGGCTATTCGACATGAAATCTACTGCTTATACGCCTAAAGTCATTGCAATCGGCCCTTTTCACCATGGTCGAAACGATTTGCTGGCTACACAGCAGTCTAAACTCCATTGTTTCCGTAATTATCTAATCCGAATAGAACGGGATGTTAAACGCCTGTTTCATAGTGGAGCTTATGATAATATTGAATTTTGA

Coding sequence (CDS)

ATGGAGTGCTTTGCTTTGGCTGCTAACAAGGGTGACGCCTTAAGGCATCAACAGGACTATTGTAAAGCTTTCTATGAGACGCCCTGGCCAACTAAGTACTCTGTGGATTTGGTGGCTGATCAGCAATCGGAAGAGGATGATCTGAGTCTTGAGTTATCCATCATAAAACTTCTGGAGGAACAGCCTCCCATCGCCGCTGCAGAATGGTGCATCTACAGGGTTCCCAAACGGCTATTCGACATGAAATCTACTGCTTATACGCCTAAAGTCATTGCAATCGGCCCTTTTCACCATGGTCGAAACGATTTGCTGGCTACACAGCAGTCTAAACTCCATTGTTTCCGTAATTATCTAATCCGAATAGAACGGGATGTTAAACGCCTGTTTCATAGTGGAGCTTATGATAATATTGAATTTTGA

Protein sequence

MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIRIERDVKRLFHSGAYDNIEF

Homology

[Close]

BLAST of MC08g_new0049 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 9.2e-05
Identity = 20/49 (40.82%), Postives = 29/49 (59.18%), Query Frame = 0


Query: 70  CIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYL 119
           CI+RVP+    +   AY PKV++IGP+H+G   L   QQ K    + +L
Sbjct: 47  CIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFL 95

[Close]

BLAST of MC08g_new0049 vs. NCBI nr
Match: XP_022132118.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 263 bits (673), Expect = 6.33e-84
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEE 60
           MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEE
Sbjct: 1   MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEE 60

Query: 61  QPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIR 120
           QPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIR
Sbjct: 61  QPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIR 120

Query: 121 IERDVK 126
           IERDVK
Sbjct: 121 IERDVK 126

[Close]

BLAST of MC08g_new0049 vs. NCBI nr
Match: XP_031744666.1 (UPF0481 protein At3g47200-like [Cucumis sativus])

HSP 1 Score: 73.2 bits (178), Expect = 1.20e-12
Identity = 37/68 (54.41%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 55  IKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCF 114
           IK   EQ P+   E  IYRVPK L  MK T Y P+VI+IGPFHHG  DL AT+  KL   
Sbjct: 31  IKSKLEQLPVVNKECKIYRVPKLLCQMKKTTYVPQVISIGPFHHGSKDLNATEPYKLQGL 90

Query: 115 RNYLIRIE 122
           RN+L R++
Sbjct: 91  RNFLRRLD 98

[Close]

BLAST of MC08g_new0049 vs. NCBI nr
Match: XP_022961913.1 (UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 73.9 bits (180), Expect = 3.10e-12
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 41  QQSEEDDLSLELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGR 100
           +Q E    ++ LSI +++++ PP+   E  I+RVPK L +M   AYTP+VI+IGPFHH R
Sbjct: 8   EQGEHPRRNVVLSIKEMIDKLPPVNV-ECSIFRVPKLLRNMNHRAYTPQVISIGPFHHYR 67

Query: 101 NDLLATQQSKLHCFRNYLIRI--ERDVKRLFHSGA 133
            DLLAT+  KL    N+L R+  ERD   L     
Sbjct: 68  KDLLATEPYKLRRCLNFLSRLGNERDSLELLKKNT 101

[Close]

BLAST of MC08g_new0049 vs. NCBI nr
Match: XP_022932942.1 (UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 73.9 bits (180), Expect = 3.14e-12
Identity = 38/71 (53.52%), Postives = 50/71 (70.42%), Query Frame = 0


Query: 52  LSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHH-GRNDLLATQQSK 111
           +S IK + EQ PI      IYRVPK L +M STA+TP+VI+IGPFHH  R+DL+ T+Q K
Sbjct: 15  VSWIKTMLEQLPIVKVGCSIYRVPKLLREMNSTAFTPQVISIGPFHHRSRDDLIVTEQYK 74

Query: 112 LHCFRNYLIRI 121
           L    N+L+R+
Sbjct: 75  LQGLANFLLRM 85

[Close]

BLAST of MC08g_new0049 vs. NCBI nr
Match: KAF4379079.1 (hypothetical protein F8388_022166 [Cannabis sativa])

HSP 1 Score: 73.6 bits (179), Expect = 4.01e-12
Identity = 33/67 (49.25%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 52  LSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKL 111
           L+ ++L  E+PP+   + CIYRVPKRL D+   AYTPKV++IGPFHHG  +L   +  K 
Sbjct: 10  LNSLRLELERPPLLFKDRCIYRVPKRLRDVNDNAYTPKVVSIGPFHHGNQNLKPMEDHKK 69

Query: 112 HCFRNYL 118
              R+YL
Sbjct: 70  RYLRHYL 76

[Close]

BLAST of MC08g_new0049 vs. ExPASy TrEMBL
Match: A0A6J1BVD4 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE=4 SV=1)

HSP 1 Score: 263 bits (673), Expect = 3.06e-84
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEE 60
           MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEE
Sbjct: 1   MECFALAANKGDALRHQQDYCKAFYETPWPTKYSVDLVADQQSEEDDLSLELSIIKLLEE 60

Query: 61  QPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIR 120
           QPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIR
Sbjct: 61  QPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIR 120

Query: 121 IERDVK 126
           IERDVK
Sbjct: 121 IERDVK 126

[Close]

BLAST of MC08g_new0049 vs. ExPASy TrEMBL
Match: A0A0A0K4A0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G068570 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.79e-13
Identity = 37/68 (54.41%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 55  IKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCF 114
           IK   EQ P+   E  IYRVPK L  MK T Y P+VI+IGPFHHG  DL AT+  KL   
Sbjct: 31  IKSKLEQLPVVNKECKIYRVPKLLCQMKKTTYVPQVISIGPFHHGSKDLNATEPYKLQGL 90

Query: 115 RNYLIRIE 122
           RN+L R++
Sbjct: 91  RNFLRRLD 98

[Close]

BLAST of MC08g_new0049 vs. ExPASy TrEMBL
Match: A0A6J1HD72 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111462539 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.50e-12
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 41  QQSEEDDLSLELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGR 100
           +Q E    ++ LSI +++++ PP+   E  I+RVPK L +M   AYTP+VI+IGPFHH R
Sbjct: 8   EQGEHPRRNVVLSIKEMIDKLPPVNV-ECSIFRVPKLLRNMNHRAYTPQVISIGPFHHYR 67

Query: 101 NDLLATQQSKLHCFRNYLIRI--ERDVKRLFHSGA 133
            DLLAT+  KL    N+L R+  ERD   L     
Sbjct: 68  KDLLATEPYKLRRCLNFLSRLGNERDSLELLKKNT 101

[Close]

BLAST of MC08g_new0049 vs. ExPASy TrEMBL
Match: A0A6J1EY59 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111439558 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.52e-12
Identity = 38/71 (53.52%), Postives = 50/71 (70.42%), Query Frame = 0


Query: 52  LSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHH-GRNDLLATQQSK 111
           +S IK + EQ PI      IYRVPK L +M STA+TP+VI+IGPFHH  R+DL+ T+Q K
Sbjct: 15  VSWIKTMLEQLPIVKVGCSIYRVPKLLREMNSTAFTPQVISIGPFHHRSRDDLIVTEQYK 74

Query: 112 LHCFRNYLIRI 121
           L    N+L+R+
Sbjct: 75  LQGLANFLLRM 85

[Close]

BLAST of MC08g_new0049 vs. ExPASy TrEMBL
Match: A0A7J6GAE7 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_022166 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.94e-12
Identity = 33/67 (49.25%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 52  LSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKL 111
           L+ ++L  E+PP+   + CIYRVPKRL D+   AYTPKV++IGPFHHG  +L   +  K 
Sbjct: 10  LNSLRLELERPPLLFKDRCIYRVPKRLRDVNDNAYTPKVVSIGPFHHGNQNLKPMEDHKK 69

Query: 112 HCFRNYL 118
              R+YL
Sbjct: 70  RYLRHYL 76

[Close]

BLAST of MC08g_new0049 vs. TAIR 10
Match: AT3G47210.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 60.1 bits (144), Expect = 1.7e-09
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 70  CIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYL--IRIERDV 126
           CI+RVPK   +M   AY PKV++IGP+HHGR  L   QQ KL     +L    ++RDV
Sbjct: 94  CIFRVPKSFAEMNPEAYKPKVVSIGPYHHGRKHLEMIQQHKLRFLHLFLRTASVDRDV 151

[Close]

BLAST of MC08g_new0049 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 54.7 bits (130), Expect = 7.0e-08
Identity = 27/103 (26.21%), Postives = 52/103 (50.49%), Query Frame = 0


Query: 30  PTKYSVDLVADQQSEEDDLSLELSIIKLLEEQPPI---AAAEW---CIYRVPKRLFDMKS 89
           P +  V+ V   + + +    E+ +I L ++   +   A   W   CIYRVP  L +  +
Sbjct: 59  PRETQVESVVSIEDKNEQKLREIWVISLNDKMKTLGDNATTSWDNLCIYRVPPYLQENDT 118

Query: 90  TAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIRIERDVK 127
            +Y P++++IGP+HHG   L+  ++ K       + R + D++
Sbjct: 119 KSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIE 161

[Close]

BLAST of MC08g_new0049 vs. TAIR 10
Match: AT5G22540.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 54.3 bits (129), Expect = 9.1e-08
Identity = 27/69 (39.13%), Postives = 42/69 (60.87%), Query Frame = 0


Query: 55  IKLLEEQPPIAAAE-WCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHC 114
           +KLL E    A +E  CI R+P+ L  +   AY PK+++IGP+HHG+  L  TQQ K   
Sbjct: 19  LKLLRES---AGSELCCIVRIPQSLARINLKAYEPKIVSIGPYHHGKEHLKMTQQHKRRF 78

Query: 115 FRNYLIRIE 123
            + ++ ++E
Sbjct: 79  LKFFVAKME 84

[Close]

BLAST of MC08g_new0049 vs. TAIR 10
Match: AT3G47250.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 53.9 bits (128), Expect = 1.2e-07
Identity = 25/61 (40.98%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 67  AEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIRIERDVK 126
           A  CI+R+P  L ++   AY PKV++IGP+H+G N L   QQ   H FR   + ++R  K
Sbjct: 59  ASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQ---HKFRFLELFVDRATK 116

Query: 127 R 128
           +
Sbjct: 119 K 116

[Close]

BLAST of MC08g_new0049 vs. TAIR 10
Match: AT3G47250.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 53.9 bits (128), Expect = 1.2e-07
Identity = 25/61 (40.98%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 67  AEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQSKLHCFRNYLIRIERDVK 126
           A  CI+R+P  L ++   AY PKV++IGP+H+G N L   QQ   H FR   + ++R  K
Sbjct: 59  ASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQ---HKFRFLELFVDRATK 116

Query: 127 R 128
           +
Sbjct: 119 K 116

The following BLAST results are available for this feature:

BLAST of MC08g_new0049 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SD53	9.2e-05	40.82	UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1	[more]

BLAST of MC08g_new0049 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132118.1	6.33e-84	100.00	UPF0481 protein At3g47200-like [Momordica charantia]	[more]
XP_031744666.1	1.20e-12	54.41	UPF0481 protein At3g47200-like [Cucumis sativus]	[more]
XP_022961913.1	3.10e-12	44.21	UPF0481 protein At3g47200-like [Cucurbita moschata]	[more]
XP_022932942.1	3.14e-12	53.52	UPF0481 protein At3g47200-like [Cucurbita moschata]	[more]
KAF4379079.1	4.01e-12	49.25	hypothetical protein F8388_022166 [Cannabis sativa]	[more]

BLAST of MC08g_new0049 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVD4	3.06e-84	100.00	UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE...	[more]
A0A0A0K4A0	5.79e-13	54.41	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G068570 PE=4 SV=1	[more]
A0A6J1HD72	1.50e-12	44.21	UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111462539 PE=...	[more]
A0A6J1EY59	1.52e-12	53.52	UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111439558 PE=...	[more]
A0A7J6GAE7	1.94e-12	49.25	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_022166 PE=4 SV=1	[more]

BLAST of MC08g_new0049 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47210.1	1.7e-09	48.28	Plant protein of unknown function (DUF247)	[more]
AT3G50160.1	7.0e-08	26.21	Plant protein of unknown function (DUF247)	[more]
AT5G22540.1	9.1e-08	39.13	Plant protein of unknown function (DUF247)	[more]
AT3G47250.1	1.2e-07	40.98	Plant protein of unknown function (DUF247)	[more]
AT3G47250.2	1.2e-07	40.98	Plant protein of unknown function (DUF247)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004158	Protein of unknown function DUF247, plant	PFAM	PF03140	DUF247	coord: 71..122 e-value: 7.8E-12 score: 45.2
None	No IPR available	PANTHER	PTHR31170:SF12	SUBFAMILY NOT NAMED	coord: 44..124
None	No IPR available	PANTHER	PTHR31170	BNAC04G53230D PROTEIN	coord: 44..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0049.1	MC08g_new0049.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane