MC08g_new0020 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g_new0020
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Protein DETOXIFICATION
Location	MC08: 1581267 .. 1583820 (-)
RNA-Seq Expression	MC08g_new0020
Synteny	MC08g_new0020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGATCTCAACCGTCCGCTGCTGATCTCGACTAGTAATAATGGAGGAGATGATGAAAATGGGAGATCAAAGTACTCAAAGGTATGGGTGGAAACTCAGCAGCTATGGGCAATCGTGGGGCCTACAATATTCGCCCGCGTCGCTAACTACTCCATGAACGTAATCACCCAAGTTTTTGCTGGTCATCTCGGCGATGTGCAGCTCGCTTCCATTTCCATCGCCAACACTGTTATTGTGGGTTTCAATTTTGGCCTCTTGGTACGTTTTTTTTCATCGTAACTTATCAATATTCATGAAGTTCACCTCAAAACAAACAAACAATGAGACAATAAATATTGTTCATCTCAAAACCAATAAGGAATGAGACAATAAATATTAGTATATTACTGATATTGGTTAACTTAGGTTGATATTAGTGTATTACTATATTAGTTATAGTAACTACTTTATTAACTTTGTTACTTCTCTTATGTTTTCAACGTGTGATTATTAACACATAACCAATGTGCGACAAAAAGGTTAATTTTTGTTGTTTGATCAGTTGGGGATGGCGAGTGCATTGGAGACGCTGTGCGGGCAGGCGTATGGGGCAAAGAAGTACCACATGTTGGGAATATACTTGCAGCGCTCTTGGATAGTGCTGTTTTTCTGTTGCTTTCTGCTGCTTCCGTTTTACGTCTACGCCGCTCCGGTTCTGAAGTGGCTGGGCCAGGATGACGAGGTGGCGGAGGAGTCGGGGCTGGTGGCAATTTGGCTGATACCCCTCCACTTCAGCTTCGCATTTCAGTTCCCGTTGCAGACGTTCTTGCAGTGCCAGCAGAAGACGCTGGTCATCGCTTGGGTTTCGCTGGCGGGGTTGATTCTGAATGTTGTCTCGAGCTGGGTTTTGATTTATGAGTGGGAACTTGGGGTGATTGGGGCAGCCATAGCTCTGGACTTGTCTTGGTGGGTTCTTGGTTTTGGATTGTACTTCTACACTGTTGGTGGTTGGTGCCCTTTGACATGGACCGGATTCTCCATTCAAGCCTTTCATGGTCTTTGGGAATTTCTCAAACTCTCTGCTGCTGCTGGCCTCATGCTTTGGTAATTTTTTCCCTCTTCAATTTTAAGTTTTTAATAGTCTATCTATAAATATTTTCTACTTTAAAGTTGTGGACCAATTCTAGAATTTACTATTTTTTTGTATGGCAAAATTTTGGTCATCCATCAATATCATTACATTTATTGCTCTGTTTGATATAGACACATGAGCATACTCGCAGGGGTCTGTCCTTACTAATTTTTGCATCAATAATTGGGTGTGCGCAGTTTGGAGAATTGGTACTTTCGGATCCTTGTATTAATGACGGGAAATCTAGAAAATGCCACCATTGCTGTGGATGCGTTATCCGTATGGTATGCCAAATTAATTAATATTTATCTCTTCATAATTCTCATTATTTCTCACAGTTTTTTCAATAAAGTGGTGGACAATTATTGTATACTATTTTGTTGGCTAAACTTGTGCAGCATGAGCATCAACGGATGGGAGATGATGATTCCTATGGCATTCTTTGCTGGCGTCGGGTACTTTTCTTTTTACCACAATAAATCTAGAACATAAATAACAAACCCTTCTGGTTTTCGCTTTAGATAGATCAAAGACACATAAGATTTAGGAATCAGAGGCAAATTGACTAACTTGAAAATGTGAATGTTAACAGAGTGAGAGTGGCAAATGAGTTGGGGGCTGGGAACGGGAAAGCCGCCAAATTTGCAACAATTGTATCGGTGGCGGAGTCGACCGGGATCGGGATTGTGATATGTGCTATTATAATGATATTTCATGGGAAGATAGCCTTGATCTTCACAAGCAGCAGCAGGGTGGTTGAAGCAGTGGATACGCTCTCCAACCTACTGGCCATCACCATTCTCTTGAACAGCGTCCAACCCGTTCTTTCAGGTTCATAAAATCATGGATAGAACTTGGCTACTCGCTAATTCAACTAAGAGAGAGAGAAATGCAAAAACAAAATTTTCTTTTAAAAAATAACTGCGTTATTAAATATGTAATAAAATATGAATGCAAATGCAGGGGTGGCGGTTGGATCGGGATGGCAATCTTGGGTTGCATACATAAATATTGGATGCTATTATATAATTGGTCTCCCTCTTGGTTTCATTATGGAATGGGTTTTCCACTCTGGAGTTTCGGTAATTCCCTTATCCTTATAATTAATCCCATTGATTTTCTCTTAATATGTAACTAATTGGGTTATATATTTTTAAAATTGAAACAGGGTATCTGGGCAGGAATGCTATATGGTGGAACTGCACTTCAGACAATAATATTAATCATCATCACAATCAGAACTGACTGGGAAAAAGAGGTAAAAAAGTTAAATGTTATAATCCAATGCCAAGTTACTTTGACTTAAAATTAATGAATTCCTGTGACTAGGAATCGAGTGTTTATTCGATTTATTTGATCTGATTTCAGGCTGAGAAAGCACAAATGCATGTAGAGAGGTGGTCAAGCCCTAAAAAGGATGAGAAGTATCTTCTCAGTTAA

mRNA sequence

ATGGCGGATCTCAACCGTCCGCTGCTGATCTCGACTAGTAATAATGGAGGAGATGATGAAAATGGGAGATCAAAGTACTCAAAGGTATGGGTGGAAACTCAGCAGCTATGGGCAATCGTGGGGCCTACAATATTCGCCCGCGTCGCTAACTACTCCATGAACGTAATCACCCAAGTTTTTGCTGGTCATCTCGGCGATGTGCAGCTCGCTTCCATTTCCATCGCCAACACTGTTATTGTGGGTTTCAATTTTGGCCTCTTGTTGGGGATGGCGAGTGCATTGGAGACGCTGTGCGGGCAGGCGTATGGGGCAAAGAAGTACCACATGTTGGGAATATACTTGCAGCGCTCTTGGATAGTGCTGTTTTTCTGTTGCTTTCTGCTGCTTCCGTTTTACGTCTACGCCGCTCCGGTTCTGAAGTGGCTGGGCCAGGATGACGAGGTGGCGGAGGAGTCGGGGCTGGTGGCAATTTGGCTGATACCCCTCCACTTCAGCTTCGCATTTCAGTTCCCGTTGCAGACGTTCTTGCAGTGCCAGCAGAAGACGCTGGTCATCGCTTGGGTTTCGCTGGCGGGGTTGATTCTGAATGTTGTCTCGAGCTGGGTTTTGATTTATGAGTGGGAACTTGGGGTGATTGGGGCAGCCATAGCTCTGGACTTGTCTTGGTGGGTTCTTGGTTTTGGATTGTACTTCTACACTGTTGGTGGTTGGTGCCCTTTGACATGGACCGGATTCTCCATTCAAGCCTTTCATGGTCTTTGGGAATTTCTCAAACTCTCTGCTGCTGCTGGCCTCATGCTTTGTTTGGAGAATTGGTACTTTCGGATCCTTGTATTAATGACGGGAAATCTAGAAAATGCCACCATTGCTGTGGATGCGTTATCCGTATGCATGAGCATCAACGGATGGGAGATGATGATTCCTATGGCATTCTTTGCTGGCGTCGGAGTGAGAGTGGCAAATGAGTTGGGGGCTGGGAACGGGAAAGCCGCCAAATTTGCAACAATTGTATCGGTGGCGGAGTCGACCGGGATCGGGATTGTGATATGTGCTATTATAATGATATTTCATGGGAAGATAGCCTTGATCTTCACAAGCAGCAGCAGGGTGGTTGAAGCAGTGGATACGCTCTCCAACCTACTGGCCATCACCATTCTCTTGAACAGCGTCCAACCCGTTCTTTCAGGAATGCTATATGGTGGAACTGCACTTCAGACAATAATATTAATCATCATCACAATCAGAACTGACTGGGAAAAAGAGGCTGAGAAAGCACAAATGCATGTAGAGAGGTGGTCAAGCCCTAAAAAGGATGAGAAGTATCTTCTCAGTTAA

Coding sequence (CDS)

ATGGCGGATCTCAACCGTCCGCTGCTGATCTCGACTAGTAATAATGGAGGAGATGATGAAAATGGGAGATCAAAGTACTCAAAGGTATGGGTGGAAACTCAGCAGCTATGGGCAATCGTGGGGCCTACAATATTCGCCCGCGTCGCTAACTACTCCATGAACGTAATCACCCAAGTTTTTGCTGGTCATCTCGGCGATGTGCAGCTCGCTTCCATTTCCATCGCCAACACTGTTATTGTGGGTTTCAATTTTGGCCTCTTGTTGGGGATGGCGAGTGCATTGGAGACGCTGTGCGGGCAGGCGTATGGGGCAAAGAAGTACCACATGTTGGGAATATACTTGCAGCGCTCTTGGATAGTGCTGTTTTTCTGTTGCTTTCTGCTGCTTCCGTTTTACGTCTACGCCGCTCCGGTTCTGAAGTGGCTGGGCCAGGATGACGAGGTGGCGGAGGAGTCGGGGCTGGTGGCAATTTGGCTGATACCCCTCCACTTCAGCTTCGCATTTCAGTTCCCGTTGCAGACGTTCTTGCAGTGCCAGCAGAAGACGCTGGTCATCGCTTGGGTTTCGCTGGCGGGGTTGATTCTGAATGTTGTCTCGAGCTGGGTTTTGATTTATGAGTGGGAACTTGGGGTGATTGGGGCAGCCATAGCTCTGGACTTGTCTTGGTGGGTTCTTGGTTTTGGATTGTACTTCTACACTGTTGGTGGTTGGTGCCCTTTGACATGGACCGGATTCTCCATTCAAGCCTTTCATGGTCTTTGGGAATTTCTCAAACTCTCTGCTGCTGCTGGCCTCATGCTTTGTTTGGAGAATTGGTACTTTCGGATCCTTGTATTAATGACGGGAAATCTAGAAAATGCCACCATTGCTGTGGATGCGTTATCCGTATGCATGAGCATCAACGGATGGGAGATGATGATTCCTATGGCATTCTTTGCTGGCGTCGGAGTGAGAGTGGCAAATGAGTTGGGGGCTGGGAACGGGAAAGCCGCCAAATTTGCAACAATTGTATCGGTGGCGGAGTCGACCGGGATCGGGATTGTGATATGTGCTATTATAATGATATTTCATGGGAAGATAGCCTTGATCTTCACAAGCAGCAGCAGGGTGGTTGAAGCAGTGGATACGCTCTCCAACCTACTGGCCATCACCATTCTCTTGAACAGCGTCCAACCCGTTCTTTCAGGAATGCTATATGGTGGAACTGCACTTCAGACAATAATATTAATCATCATCACAATCAGAACTGACTGGGAAAAAGAGGCTGAGAAAGCACAAATGCATGTAGAGAGGTGGTCAAGCCCTAAAAAGGATGAGAAGTATCTTCTCAGTTAA

Protein sequence

MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPKKDEKYLLS

Homology

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BLAST of MC08g_new0020 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-146
Identity = 268/463 (57.88%), Postives = 335/463 (72.35%), Query Frame = 0


Query: 19  DENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGDVQLASISIANTV 78
           +E+G     ++ VET++LW IVGP IF+RV  YSM VITQ FAGHLGD++LA+ISI N V
Sbjct: 24  EEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLGDLELAAISIVNNV 83

Query: 79  IVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCFLLLPFYVYAAPV 138
            VGFNFGLLLGMASALETLCGQA+GAKKYHMLG+Y+QRSWIVLFFCC LLLP Y++  PV
Sbjct: 84  TVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCCVLLLPTYIFTTPV 143

Query: 139 LKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIAWVSLAGLILNVV 198
           LK+LGQ D++AE SG+VAIW+IPLHF+F   FPLQ FLQCQ K  V A+ +   L+++++
Sbjct: 144 LKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVTAYAAAVALVVHIL 203

Query: 199 SSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFSIQAFHGLWEFLK 258
             W+ +   +LGV+G    + +SWWV    L  Y+  G CPLTWTG S +A  GLWEFLK
Sbjct: 204 VCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTWTGLSSEALTGLWEFLK 263

Query: 259 LSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMMIPMAFFAGVGVR 318
           LSA++G+MLCLENWY+RIL++MTGNL+NA IAVD+LS+CM+INGWEMMIP+AFFAG GVR
Sbjct: 264 LSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAINGWEMMIPLAFFAGTGVR 323

Query: 319 VANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTSSSRVVEAVDTLS 378
           VANELGAGNGK A+FATIVSV +S  IG+    +IM+ H +IA IF+SS  V++AV+ LS
Sbjct: 324 VANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFSSSVAVLDAVNKLS 383

Query: 379 NLLAITILLNSVQPVLS------------------------------------------- 438
            LLA T+LLNSVQPVLS                                           
Sbjct: 384 LLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFLMGWGFKLGVMGIW 443

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BLAST of MC08g_new0020 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 4.5e-138
Identity = 248/457 (54.27%), Postives = 328/457 (71.77%), Query Frame = 0


Query: 20  ENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGDVQLASISIANTVI 79
           E G     ++W+ET+++W IVGP+IF  +A YS+ +ITQ FAGHLGD++LA+ISI N   
Sbjct: 26  EEGGGMKREIWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFT 85

Query: 80  VGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCFLLLPFYVYAAPVL 139
           +GFN+GLLLGMASALETLCGQA+GA++Y+MLG+Y+QR WI+LF CC LLLP Y++A P+L
Sbjct: 86  LGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPIL 145

Query: 140 KWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIAWVSLAGLILNVVS 199
           K++GQ D++AE +G +A+W+IP+HF+FAF FPL  FLQCQ K  VIA  +   L ++++ 
Sbjct: 146 KFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILV 205

Query: 200 SWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFSIQAFHGLWEFLKL 259
            W  +Y ++LG+IG   ++++ WW+  F L+ Y+  G C LTWTGFS +AF GL E  KL
Sbjct: 206 CWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKL 265

Query: 260 SAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMMIPMAFFAGVGVRV 319
           SA++G+MLCLENWY++IL+LMTGNL NA IAVD+LS+CMS+NGWEMMIP+AFFAG GVRV
Sbjct: 266 SASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRV 325

Query: 320 ANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTSSSRVVEAVDTLSN 379
           ANELGAGNGK A+FATIVS+  S  IG+    II+IFH +I  IF+SS  V+ AVD LS 
Sbjct: 326 ANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSV 385

Query: 380 LLAITILLNSVQPVLS-------------------------------------------- 433
           LLA T+LLNSVQPVLS                                            
Sbjct: 386 LLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVKGIWA 445

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BLAST of MC08g_new0020 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.1e-133
Identity = 250/471 (53.08%), Postives = 328/471 (69.64%), Query Frame = 0


Query: 7   PLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGD 66
           PLL     N  ++ENG  K  ++W+ET++LW IVGP IF RV    + VITQ FAGHLG+
Sbjct: 18  PLL--KDQNVAEEENGEIK-KEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGE 77

Query: 67  VQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCF 126
           ++LA+ISI N VI+GFN+ L +GMA+ALETLCGQA+GAKKY M G+YLQRSWIVLF    
Sbjct: 78  LELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSI 137

Query: 127 LLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIA 186
           LLLP Y++A P+LK++GQ D++AE SG++++W IP HFSFAF FP+  FLQCQ K  VIA
Sbjct: 138 LLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIA 197

Query: 187 WVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFS 246
             S   L++++   W+ +Y  ELGVIG     ++SWW+  F L+ YT  G CPLTWTGFS
Sbjct: 198 ISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFS 257

Query: 247 IQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMM 306
           +++F  LWEF KLSA++G+M+CLENWY+R+L++MTGNLE+A I VD++S+CMSING EMM
Sbjct: 258 MESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMM 317

Query: 307 IPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTS 366
           +P+AFFAG  VRVANELGAGNGK A+FA I+SV +S  IGI+I  +I     +I  +F+S
Sbjct: 318 VPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSS 377

Query: 367 SSRVVEAVDTLSNLLAITILLNSVQPVLS------------------------------- 426
           S  V++AV+ LS LL+  ILLNSVQPVLS                               
Sbjct: 378 SETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVM 437

Query: 427 -------------GMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWS 434
                        GM++GGT +QT+ILI IT+R DWEKEA+ A++ V +WS
Sbjct: 438 GWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

[Close]

BLAST of MC08g_new0020 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 4.7e-103
Identity = 201/477 (42.14%), Postives = 293/477 (61.43%), Query Frame = 0


Query: 3   DLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAG 62
           +L   LL  T+ NGG++++      KVW+E+++LW +  P IF R + + +++I+Q F G
Sbjct: 7   ELTAALLKKTAENGGEEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIG 66

Query: 63  HLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLF 122
           HLG ++LA+ SI  TV++ F+ G+LLGMASALETLCGQAYGAK+ HMLGIYLQRSWIVL 
Sbjct: 67  HLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLT 126

Query: 123 FCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKT 182
            C   L P Y+++ P+L  LGQ++ +   + ++A+W+I ++FSF   F  Q FLQ Q K 
Sbjct: 127 GCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKN 186

Query: 183 LVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTW 242
            +IA+V+   L ++V  SW+L+  +  G+ GA  +  +++W+       +   G C  TW
Sbjct: 187 KIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTW 246

Query: 243 TGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSING 302
            GFS+ AF  LW   KLS ++G MLCLE WY  ILVL+TGNL+NA +A+DAL++C++ING
Sbjct: 247 RGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLNING 306

Query: 303 WEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIAL 362
            EMMI + F A   VRV+NELG+GN K AKFAT+ +V  S  +GIV+  + +   G+++ 
Sbjct: 307 LEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSY 366

Query: 363 IFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGMLYG---------------------- 422
           IFT+S  V   V  LS LLA +IL+NSVQPVLSG+  G                      
Sbjct: 367 IFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPI 426

Query: 423 ---------------------GTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK 437
                                G  +QT +L ++T+RTDW+++   +   + RW  P+
Sbjct: 427 GIILGYVVGLQVKGVWIGMLFGIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRWVVPE 483

[Close]

BLAST of MC08g_new0020 vs. ExPASy Swiss-Prot
Match: F4HPH1 (Protein DETOXIFICATION 22 OS=Arabidopsis thaliana OX=3702 GN=DTX22 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 8.0e-103
Identity = 202/485 (41.65%), Postives = 297/485 (61.24%), Query Frame = 0


Query: 3   DLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAG 62
           +L   LL  T+ NGG++ +      KVW+E+++LW +  P+IF + + Y ++++TQ F G
Sbjct: 7   ELTAALLKKTTENGGEENDELGLKEKVWIESKKLWVVAAPSIFTKFSTYGVSLVTQGFVG 66

Query: 63  HLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLF 122
           H+G  +LA+ SI  TV++ F+ G+LLGMASAL TLCGQAYGAK+YHMLGI+LQRSWIVL 
Sbjct: 67  HIGPTELAAYSITFTVLLRFSNGILLGMASALGTLCGQAYGAKQYHMLGIHLQRSWIVLT 126

Query: 123 FCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKT 182
            C   ++P ++++ P+L  LGQ+D +   + ++A+WLI ++F+F   F  Q FLQ Q K 
Sbjct: 127 GCTICIMPIFIFSGPILLALGQEDHIVRVARVIALWLIAINFTFVPAFTCQIFLQSQSKN 186

Query: 183 LVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTW 242
            +IA+VS   L L+V  SW+L+  +  G+ GA  +  +++W+       Y   G C  TW
Sbjct: 187 KIIAYVSAVTLGLHVFFSWLLVVHFNFGITGAMTSTLVAFWMPNIVQLLYVTSGGCKDTW 246

Query: 243 TGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSING 302
            GF++ AF  LW   KLS ++G M+CLE WY  ILVL+TGNL+NA +A+DAL++C+++N 
Sbjct: 247 RGFTMLAFKDLWPVFKLSLSSGGMVCLELWYNSILVLLTGNLKNAEVAIDALAICINVNA 306

Query: 303 WEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIAL 362
            +MMI + F A V VRV+NELG GN + AKFATIV+V  S  IG+V+  + +   G+I+ 
Sbjct: 307 LQMMIALGFLAAVSVRVSNELGRGNPEGAKFATIVAVFTSLSIGLVLFFVFLFLRGRISY 366

Query: 363 IFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGMLYG---------------------- 422
           IFT+S  V   V  LS LLA +ILLNSVQPVLSG+  G                      
Sbjct: 367 IFTTSEAVAAEVADLSPLLAFSILLNSVQPVLSGVAVGAGWQGYVAYINLACYYLLGIPV 426

Query: 423 ---------------------GTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPKKDE 445
                                G  +QT +L I+T+RTDW+++   +  ++ RW  P+  +
Sbjct: 427 GLVLGYVVGLQVKGVWIGMLFGIFVQTCVLTIMTLRTDWDQQVSTSLKNINRWVVPESRD 486

[Close]

BLAST of MC08g_new0020 vs. NCBI nr
Match: XP_022131672.1 (protein DETOXIFICATION 27-like [Momordica charantia] >XP_022131673.1 protein DETOXIFICATION 27-like [Momordica charantia] >XP_022131674.1 protein DETOXIFICATION 27-like [Momordica charantia] >XP_022131675.1 protein DETOXIFICATION 27-like [Momordica charantia])

HSP 1 Score: 847 bits (2188), Expect = 1.71e-308
Identity = 444/488 (90.98%), Postives = 444/488 (90.98%), Query Frame = 0


Query: 1   MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVF 60
           MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVF
Sbjct: 1   MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVF 60

Query: 61  AGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIV 120
           AGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIV
Sbjct: 61  AGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIV 120

Query: 121 LFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQ 180
           LFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQ
Sbjct: 121 LFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQ 180

Query: 181 KTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPL 240
           KTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPL
Sbjct: 181 KTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPL 240

Query: 241 TWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSI 300
           TWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSI
Sbjct: 241 TWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSI 300

Query: 301 NGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKI 360
           NGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKI
Sbjct: 301 NGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKI 360

Query: 361 ALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG------------------------ 420
           ALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG                        
Sbjct: 361 ALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGVAVGSGWQSWVAYINIGCYYIIGL 420

Query: 421 --------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK 444
                               MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK
Sbjct: 421 PLGFIMEWVFHSGVSGIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK 480

[Close]

BLAST of MC08g_new0020 vs. NCBI nr
Match: XP_022131671.1 (protein DETOXIFICATION 27-like [Momordica charantia])

HSP 1 Score: 734 bits (1895), Expect = 6.91e-264
Identity = 386/491 (78.62%), Postives = 413/491 (84.11%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK----YSKVWVETQQLWAIVGPTIFARVANYSMNVIT 61
           +D N+PLL +      +DE+G+ +    +SK+W ETQQLW IVGPTIFARVA ++MNVIT
Sbjct: 3   SDHNQPLLSN------EDEDGKWRSQDFWSKLWRETQQLWVIVGPTIFARVATFTMNVIT 62

Query: 62  QVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRS 121
           QVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRS
Sbjct: 63  QVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRS 122

Query: 122 WIVLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQ 181
           WIVLF CCFLLLPFYVY  PVLK LG +D+VAEESG+VAIWLIPLHFSFAFQFPLQTFLQ
Sbjct: 123 WIVLFLCCFLLLPFYVYTTPVLKLLGHEDDVAEESGVVAIWLIPLHFSFAFQFPLQTFLQ 182

Query: 182 CQQKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGW 241
           CQ KT+VIAWVSL GLI+NVVSSWVLIYEWELGVIGAAIALD+SWWVLGFGLYFYTVGGW
Sbjct: 183 CQHKTMVIAWVSLVGLIVNVVSSWVLIYEWELGVIGAAIALDVSWWVLGFGLYFYTVGGW 242

Query: 242 CPLTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVC 301
           CPLTWTGFSIQAFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENAT AVDALSVC
Sbjct: 243 CPLTWTGFSIQAFHGLWEFLKLSTAAGLMLCLENWYFRILVLMTGNLENATTAVDALSVC 302

Query: 302 MSINGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFH 361
           MSINGWEMMIP+AFFAG+GVRVANELGAGNGK AKFATIVSVA+S  IG+VIC +IM FH
Sbjct: 303 MSINGWEMMIPIAFFAGIGVRVANELGAGNGKGAKFATIVSVAQSILIGVVICVVIMTFH 362

Query: 362 GKIALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG--------------------- 421
            KIALIFTSSS VVEAVD+LSNLLAITILLNS+QPVLSG                     
Sbjct: 363 DKIALIFTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYI 422

Query: 422 -----------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWS 444
                                  MLYGGTALQTIILIIITIRTDWEKEAEKAQMHV+ WS
Sbjct: 423 IGLPLGFIMEWVFHSGVSGIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVDNWS 482

[Close]

BLAST of MC08g_new0020 vs. NCBI nr
Match: XP_022997119.1 (protein DETOXIFICATION 27-like [Cucurbita maxima])

HSP 1 Score: 697 bits (1800), Expect = 2.57e-249
Identity = 365/489 (74.64%), Postives = 406/489 (83.03%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK--YSKVWVETQQLWAIVGPTIFARVANYSMNVITQV 61
           +DLN+PL+       G+D   RS+  +SK+WVETQ+LW IVGPTIFARVA +SMNVITQ 
Sbjct: 11  SDLNQPLIRR-----GEDRTRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMNVITQA 70

Query: 62  FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI 121
           FAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSWI
Sbjct: 71  FAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWI 130

Query: 122 VLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQ 181
           +LF CCFLLLPFYVYA PVLK LGQ ++VAE SG+VAIWLIPLHFSFAFQFPLQ FLQCQ
Sbjct: 131 MLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQ 190

Query: 182 QKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCP 241
            KTLVIAWVSL GL++NVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWCP
Sbjct: 191 HKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCP 250

Query: 242 LTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 301
           LTWTGFS++AFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS
Sbjct: 251 LTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 310

Query: 302 INGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGK 361
           INGWEMMIP+AF AGVGVRVANELGAGNGKAAKFATIVSVA+ST IGI IC +I++FHGK
Sbjct: 311 INGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGK 370

Query: 362 IALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG----------------------- 421
           IALIF+SS  VVEAVD+LSNLLAITILLNS+QPVLSG                       
Sbjct: 371 IALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIG 430

Query: 422 ---------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSP 444
                                ML+GG+A QT+IL+IITIRTDWEKEAEKA  HVE WS+P
Sbjct: 431 LPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILMIITIRTDWEKEAEKALKHVEEWSTP 490

[Close]

BLAST of MC08g_new0020 vs. NCBI nr
Match: KAG7029651.1 (Protein DETOXIFICATION 27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 697 bits (1798), Expect = 5.18e-249
Identity = 367/489 (75.05%), Postives = 404/489 (82.62%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK--YSKVWVETQQLWAIVGPTIFARVANYSMNVITQV 61
           ADLN  L+       G+D   RS+  +SK+WVETQ+LW IVGPTIFARVA +SMNVITQ 
Sbjct: 11  ADLNEALIRR-----GEDRPRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMNVITQA 70

Query: 62  FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI 121
           FAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSWI
Sbjct: 71  FAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWI 130

Query: 122 VLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQ 181
           +LF CCFLLLPFYVYA PVLK LGQ ++VAE SG+VAIWLIPLHFSFAFQFPLQ FLQCQ
Sbjct: 131 MLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQ 190

Query: 182 QKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCP 241
            KTLVIAWVSL GL++NVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWCP
Sbjct: 191 HKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCP 250

Query: 242 LTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 301
           LTWTGFS++AFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS
Sbjct: 251 LTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 310

Query: 302 INGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGK 361
           INGWEMMIP+AF AGVGVRVANELGAGNGKAAKFATIVSVA+ST IGI IC +I++FHGK
Sbjct: 311 INGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGK 370

Query: 362 IALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG----------------------- 421
           IALIF+SS  VVEAVD+LSNLLAITILLNS+QPVLSG                       
Sbjct: 371 IALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIG 430

Query: 422 ---------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSP 444
                                ML+GG+A QT+ILIIITIRTDWEKEAEKA  HVE WS+P
Sbjct: 431 LPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAEKALKHVEEWSTP 490

[Close]

BLAST of MC08g_new0020 vs. NCBI nr
Match: XP_023545612.1 (protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 696 bits (1796), Expect = 1.04e-248
Identity = 365/490 (74.49%), Postives = 404/490 (82.45%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK---YSKVWVETQQLWAIVGPTIFARVANYSMNVITQ 61
           ADLN+ L+      G D++  R     +SK+WVETQ+LW IVGPTIFARVA +SMN+ITQ
Sbjct: 11  ADLNQALI------GRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQ 70

Query: 62  VFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSW 121
            FAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSW
Sbjct: 71  AFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSW 130

Query: 122 IVLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQC 181
           I+LF CCFLLLPFYVYA PVLK LGQ D+VAE SG+VAIWLIPLHFSFAFQFPLQ FLQC
Sbjct: 131 IMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQC 190

Query: 182 QQKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWC 241
           Q KTLVIAWVSL GL++NVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWC
Sbjct: 191 QHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWC 250

Query: 242 PLTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCM 301
           PLTWTGFS++AFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCM
Sbjct: 251 PLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCM 310

Query: 302 SINGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHG 361
           SINGWEMMIP+AF AGVGVRVANELGAGNGKAAKFATIVSVA+ST IGI IC +I++FHG
Sbjct: 311 SINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHG 370

Query: 362 KIALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG---------------------- 421
           KIALIF+SS+ VVEAVD+LSNLLAITILLNS+QPVLSG                      
Sbjct: 371 KIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGI 430

Query: 422 ----------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSS 444
                                 ML+GG+A QT+ILIIITIRTDWEKEAE A  HVE WS+
Sbjct: 431 GLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWST 490

[Close]

BLAST of MC08g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1BQC7 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004792 PE=3 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 8.26e-309
Identity = 444/488 (90.98%), Postives = 444/488 (90.98%), Query Frame = 0


Query: 1   MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVF 60
           MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVF
Sbjct: 1   MADLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVF 60

Query: 61  AGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIV 120
           AGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIV
Sbjct: 61  AGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIV 120

Query: 121 LFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQ 180
           LFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQ
Sbjct: 121 LFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQ 180

Query: 181 KTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPL 240
           KTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPL
Sbjct: 181 KTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPL 240

Query: 241 TWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSI 300
           TWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSI
Sbjct: 241 TWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSI 300

Query: 301 NGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKI 360
           NGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKI
Sbjct: 301 NGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKI 360

Query: 361 ALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG------------------------ 420
           ALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG                        
Sbjct: 361 ALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGVAVGSGWQSWVAYINIGCYYIIGL 420

Query: 421 --------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK 444
                               MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK
Sbjct: 421 PLGFIMEWVFHSGVSGIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK 480

[Close]

BLAST of MC08g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1BQ61 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004791 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 3.34e-264
Identity = 386/491 (78.62%), Postives = 413/491 (84.11%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK----YSKVWVETQQLWAIVGPTIFARVANYSMNVIT 61
           +D N+PLL +      +DE+G+ +    +SK+W ETQQLW IVGPTIFARVA ++MNVIT
Sbjct: 3   SDHNQPLLSN------EDEDGKWRSQDFWSKLWRETQQLWVIVGPTIFARVATFTMNVIT 62

Query: 62  QVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRS 121
           QVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRS
Sbjct: 63  QVFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRS 122

Query: 122 WIVLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQ 181
           WIVLF CCFLLLPFYVY  PVLK LG +D+VAEESG+VAIWLIPLHFSFAFQFPLQTFLQ
Sbjct: 123 WIVLFLCCFLLLPFYVYTTPVLKLLGHEDDVAEESGVVAIWLIPLHFSFAFQFPLQTFLQ 182

Query: 182 CQQKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGW 241
           CQ KT+VIAWVSL GLI+NVVSSWVLIYEWELGVIGAAIALD+SWWVLGFGLYFYTVGGW
Sbjct: 183 CQHKTMVIAWVSLVGLIVNVVSSWVLIYEWELGVIGAAIALDVSWWVLGFGLYFYTVGGW 242

Query: 242 CPLTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVC 301
           CPLTWTGFSIQAFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENAT AVDALSVC
Sbjct: 243 CPLTWTGFSIQAFHGLWEFLKLSTAAGLMLCLENWYFRILVLMTGNLENATTAVDALSVC 302

Query: 302 MSINGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFH 361
           MSINGWEMMIP+AFFAG+GVRVANELGAGNGK AKFATIVSVA+S  IG+VIC +IM FH
Sbjct: 303 MSINGWEMMIPIAFFAGIGVRVANELGAGNGKGAKFATIVSVAQSILIGVVICVVIMTFH 362

Query: 362 GKIALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG--------------------- 421
            KIALIFTSSS VVEAVD+LSNLLAITILLNS+QPVLSG                     
Sbjct: 363 DKIALIFTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYI 422

Query: 422 -----------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWS 444
                                  MLYGGTALQTIILIIITIRTDWEKEAEKAQMHV+ WS
Sbjct: 423 IGLPLGFIMEWVFHSGVSGIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVDNWS 482

[Close]

BLAST of MC08g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1KAJ6 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111492126 PE=3 SV=1)

HSP 1 Score: 697 bits (1800), Expect = 1.24e-249
Identity = 365/489 (74.64%), Postives = 406/489 (83.03%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK--YSKVWVETQQLWAIVGPTIFARVANYSMNVITQV 61
           +DLN+PL+       G+D   RS+  +SK+WVETQ+LW IVGPTIFARVA +SMNVITQ 
Sbjct: 11  SDLNQPLIRR-----GEDRTRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMNVITQA 70

Query: 62  FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI 121
           FAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSWI
Sbjct: 71  FAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWI 130

Query: 122 VLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQ 181
           +LF CCFLLLPFYVYA PVLK LGQ ++VAE SG+VAIWLIPLHFSFAFQFPLQ FLQCQ
Sbjct: 131 MLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQ 190

Query: 182 QKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCP 241
            KTLVIAWVSL GL++NVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWCP
Sbjct: 191 HKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCP 250

Query: 242 LTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 301
           LTWTGFS++AFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS
Sbjct: 251 LTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 310

Query: 302 INGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGK 361
           INGWEMMIP+AF AGVGVRVANELGAGNGKAAKFATIVSVA+ST IGI IC +I++FHGK
Sbjct: 311 INGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGK 370

Query: 362 IALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG----------------------- 421
           IALIF+SS  VVEAVD+LSNLLAITILLNS+QPVLSG                       
Sbjct: 371 IALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIG 430

Query: 422 ---------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSP 444
                                ML+GG+A QT+IL+IITIRTDWEKEAEKA  HVE WS+P
Sbjct: 431 LPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILMIITIRTDWEKEAEKALKHVEEWSTP 490

[Close]

BLAST of MC08g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1HEV9 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111462892 PE=3 SV=1)

HSP 1 Score: 692 bits (1786), Expect = 1.68e-247
Identity = 365/489 (74.64%), Postives = 403/489 (82.41%), Query Frame = 0


Query: 2   ADLNRPLLISTSNNGGDDENGRSK--YSKVWVETQQLWAIVGPTIFARVANYSMNVITQV 61
           ADLN+ L+        +D   RS+  +SK+WVETQ+LW IVGPTIFARVA +SMNVITQ 
Sbjct: 11  ADLNQALIRRD-----EDRPRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMNVITQA 70

Query: 62  FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI 121
           FAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSWI
Sbjct: 71  FAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWI 130

Query: 122 VLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQ 181
           +LF CCFLLLPFYVYA PVLK LGQ ++VAE SG+VAIWLIPLHFSFAFQFPLQ FLQCQ
Sbjct: 131 MLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQ 190

Query: 182 QKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCP 241
            KTLVIAWVSL GL++NVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWCP
Sbjct: 191 HKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCP 250

Query: 242 LTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 301
           LTWTGFS++AFHGLWEFLKLS AAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS
Sbjct: 251 LTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 310

Query: 302 INGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGK 361
           INGWEMMIP+AF AGVGVRVANELGAGNGKAAKFATIVSVA+ST IGI IC +I++FHGK
Sbjct: 311 INGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICVVIVLFHGK 370

Query: 362 IALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG----------------------- 421
           IALIF+SS  VVEAVD+LSNLLAITILLNS+QPVLSG                       
Sbjct: 371 IALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIG 430

Query: 422 ---------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSP 444
                                ML+GG+A QT+ILIIITIRTDWEKEA KA  HVE WS+P
Sbjct: 431 LPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAVKALKHVEEWSTP 490

[Close]

BLAST of MC08g_new0020 vs. ExPASy TrEMBL
Match: A0A6J1BQ65 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004794 PE=3 SV=1)

HSP 1 Score: 680 bits (1754), Expect = 1.54e-242
Identity = 352/489 (71.98%), Postives = 400/489 (81.80%), Query Frame = 0


Query: 4   LNRPLLISTSNNGGDDENGRSK----YSKVWVETQQLWAIVGPTIFARVANYSMNVITQV 63
           LN+PL+IS+ +     E+G  +     SK+WVETQQLW IVGP+IF+RVA YSMN+ITQ 
Sbjct: 12  LNQPLIISSKDKSRATESGEDRREDFLSKLWVETQQLWLIVGPSIFSRVAGYSMNIITQA 71

Query: 64  FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI 123
           FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI
Sbjct: 72  FAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWI 131

Query: 124 VLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQ 183
           VL  CCFLLLPFY YA PVLK LGQ D+VAE+SG+VA+WLIPLHFSFAFQFPLQ FLQ Q
Sbjct: 132 VLCLCCFLLLPFYFYATPVLKLLGQPDDVAEQSGVVALWLIPLHFSFAFQFPLQRFLQSQ 191

Query: 184 QKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCP 243
            KT VIAWVSL GL++N+V+SWVL+Y WE GVIGAAIALD+SWWVL FGLY YTVGGWCP
Sbjct: 192 LKTQVIAWVSLLGLLVNIVTSWVLMYVWEFGVIGAAIALDISWWVLVFGLYIYTVGGWCP 251

Query: 244 LTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMS 303
           LTWTGFS+QAF GLW+F KLSAAAGLMLC ENWY+RILVLMTGNL++AT+AVDALS+CMS
Sbjct: 252 LTWTGFSVQAFQGLWDFTKLSAAAGLMLCSENWYYRILVLMTGNLKDATVAVDALSICMS 311

Query: 304 INGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGK 363
           INGWEMM+P+AFFAG+GVRVANELGAGNGK AKFATIVSVA+ST IG+VIC +IMI H K
Sbjct: 312 INGWEMMVPLAFFAGIGVRVANELGAGNGKGAKFATIVSVAQSTVIGVVICVVIMILHDK 371

Query: 364 IALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSG----------------------- 423
           IALIFTSSS VVEAVD+LSNLLAITILLNS+QPVLSG                       
Sbjct: 372 IALIFTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYLIG 431

Query: 424 ---------------------MLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSSP 444
                                M++GGTA+QTIIL+IIT+RT+WE+EA+ A  +VE WSSP
Sbjct: 432 LPLGFVMEWVFHSGVLGIWGGMIFGGTAIQTIILVIITMRTNWEQEAQNALKNVEDWSSP 491

[Close]

BLAST of MC08g_new0020 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.4e-147
Identity = 268/463 (57.88%), Postives = 335/463 (72.35%), Query Frame = 0


Query: 19  DENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGDVQLASISIANTV 78
           +E+G     ++ VET++LW IVGP IF+RV  YSM VITQ FAGHLGD++LA+ISI N V
Sbjct: 24  EEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLGDLELAAISIVNNV 83

Query: 79  IVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCFLLLPFYVYAAPV 138
            VGFNFGLLLGMASALETLCGQA+GAKKYHMLG+Y+QRSWIVLFFCC LLLP Y++  PV
Sbjct: 84  TVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCCVLLLPTYIFTTPV 143

Query: 139 LKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIAWVSLAGLILNVV 198
           LK+LGQ D++AE SG+VAIW+IPLHF+F   FPLQ FLQCQ K  V A+ +   L+++++
Sbjct: 144 LKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVTAYAAAVALVVHIL 203

Query: 199 SSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFSIQAFHGLWEFLK 258
             W+ +   +LGV+G    + +SWWV    L  Y+  G CPLTWTG S +A  GLWEFLK
Sbjct: 204 VCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTWTGLSSEALTGLWEFLK 263

Query: 259 LSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMMIPMAFFAGVGVR 318
           LSA++G+MLCLENWY+RIL++MTGNL+NA IAVD+LS+CM+INGWEMMIP+AFFAG GVR
Sbjct: 264 LSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAINGWEMMIPLAFFAGTGVR 323

Query: 319 VANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTSSSRVVEAVDTLS 378
           VANELGAGNGK A+FATIVSV +S  IG+    +IM+ H +IA IF+SS  V++AV+ LS
Sbjct: 324 VANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFSSSVAVLDAVNKLS 383

Query: 379 NLLAITILLNSVQPVLS------------------------------------------- 438
            LLA T+LLNSVQPVLS                                           
Sbjct: 384 LLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFLMGWGFKLGVMGIW 443

[Close]

BLAST of MC08g_new0020 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 492.7 bits (1267), Expect = 3.2e-139
Identity = 248/457 (54.27%), Postives = 328/457 (71.77%), Query Frame = 0


Query: 20  ENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGDVQLASISIANTVI 79
           E G     ++W+ET+++W IVGP+IF  +A YS+ +ITQ FAGHLGD++LA+ISI N   
Sbjct: 26  EEGGGMKREIWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFT 85

Query: 80  VGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCFLLLPFYVYAAPVL 139
           +GFN+GLLLGMASALETLCGQA+GA++Y+MLG+Y+QR WI+LF CC LLLP Y++A P+L
Sbjct: 86  LGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPIL 145

Query: 140 KWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIAWVSLAGLILNVVS 199
           K++GQ D++AE +G +A+W+IP+HF+FAF FPL  FLQCQ K  VIA  +   L ++++ 
Sbjct: 146 KFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILV 205

Query: 200 SWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFSIQAFHGLWEFLKL 259
            W  +Y ++LG+IG   ++++ WW+  F L+ Y+  G C LTWTGFS +AF GL E  KL
Sbjct: 206 CWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKL 265

Query: 260 SAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMMIPMAFFAGVGVRV 319
           SA++G+MLCLENWY++IL+LMTGNL NA IAVD+LS+CMS+NGWEMMIP+AFFAG GVRV
Sbjct: 266 SASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRV 325

Query: 320 ANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTSSSRVVEAVDTLSN 379
           ANELGAGNGK A+FATIVS+  S  IG+    II+IFH +I  IF+SS  V+ AVD LS 
Sbjct: 326 ANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSV 385

Query: 380 LLAITILLNSVQPVLS-------------------------------------------- 433
           LLA T+LLNSVQPVLS                                            
Sbjct: 386 LLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVKGIWA 445

[Close]

BLAST of MC08g_new0020 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 478.0 bits (1229), Expect = 8.1e-135
Identity = 250/471 (53.08%), Postives = 328/471 (69.64%), Query Frame = 0


Query: 7   PLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAGHLGD 66
           PLL     N  ++ENG  K  ++W+ET++LW IVGP IF RV    + VITQ FAGHLG+
Sbjct: 18  PLL--KDQNVAEEENGEIK-KEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGE 77

Query: 67  VQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFFCCF 126
           ++LA+ISI N VI+GFN+ L +GMA+ALETLCGQA+GAKKY M G+YLQRSWIVLF    
Sbjct: 78  LELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSI 137

Query: 127 LLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKTLVIA 186
           LLLP Y++A P+LK++GQ D++AE SG++++W IP HFSFAF FP+  FLQCQ K  VIA
Sbjct: 138 LLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIA 197

Query: 187 WVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTWTGFS 246
             S   L++++   W+ +Y  ELGVIG     ++SWW+  F L+ YT  G CPLTWTGFS
Sbjct: 198 ISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFS 257

Query: 247 IQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSINGWEMM 306
           +++F  LWEF KLSA++G+M+CLENWY+R+L++MTGNLE+A I VD++S+CMSING EMM
Sbjct: 258 MESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMM 317

Query: 307 IPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIALIFTS 366
           +P+AFFAG  VRVANELGAGNGK A+FA I+SV +S  IGI+I  +I     +I  +F+S
Sbjct: 318 VPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSS 377

Query: 367 SSRVVEAVDTLSNLLAITILLNSVQPVLS------------------------------- 426
           S  V++AV+ LS LL+  ILLNSVQPVLS                               
Sbjct: 378 SETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVM 437

Query: 427 -------------GMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWS 434
                        GM++GGT +QT+ILI IT+R DWEKEA+ A++ V +WS
Sbjct: 438 GWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

[Close]

BLAST of MC08g_new0020 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 376.3 bits (965), Expect = 3.3e-104
Identity = 201/477 (42.14%), Postives = 293/477 (61.43%), Query Frame = 0


Query: 3   DLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAG 62
           +L   LL  T+ NGG++++      KVW+E+++LW +  P IF R + + +++I+Q F G
Sbjct: 7   ELTAALLKKTAENGGEEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIG 66

Query: 63  HLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLF 122
           HLG ++LA+ SI  TV++ F+ G+LLGMASALETLCGQAYGAK+ HMLGIYLQRSWIVL 
Sbjct: 67  HLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLT 126

Query: 123 FCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKT 182
            C   L P Y+++ P+L  LGQ++ +   + ++A+W+I ++FSF   F  Q FLQ Q K 
Sbjct: 127 GCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKN 186

Query: 183 LVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTW 242
            +IA+V+   L ++V  SW+L+  +  G+ GA  +  +++W+       +   G C  TW
Sbjct: 187 KIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTW 246

Query: 243 TGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSING 302
            GFS+ AF  LW   KLS ++G MLCLE WY  ILVL+TGNL+NA +A+DAL++C++ING
Sbjct: 247 RGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLNING 306

Query: 303 WEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIAL 362
            EMMI + F A   VRV+NELG+GN K AKFAT+ +V  S  +GIV+  + +   G+++ 
Sbjct: 307 LEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSY 366

Query: 363 IFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGMLYG---------------------- 422
           IFT+S  V   V  LS LLA +IL+NSVQPVLSG+  G                      
Sbjct: 367 IFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPI 426

Query: 423 ---------------------GTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPK 437
                                G  +QT +L ++T+RTDW+++   +   + RW  P+
Sbjct: 427 GIILGYVVGLQVKGVWIGMLFGIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRWVVPE 483

[Close]

BLAST of MC08g_new0020 vs. TAIR 10
Match: AT1G33090.1 (MATE efflux family protein )

HSP 1 Score: 375.6 bits (963), Expect = 5.7e-104
Identity = 202/485 (41.65%), Postives = 297/485 (61.24%), Query Frame = 0


Query: 3   DLNRPLLISTSNNGGDDENGRSKYSKVWVETQQLWAIVGPTIFARVANYSMNVITQVFAG 62
           +L   LL  T+ NGG++ +      KVW+E+++LW +  P+IF + + Y ++++TQ F G
Sbjct: 7   ELTAALLKKTTENGGEENDELGLKEKVWIESKKLWVVAAPSIFTKFSTYGVSLVTQGFVG 66

Query: 63  HLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLF 122
           H+G  +LA+ SI  TV++ F+ G+LLGMASAL TLCGQAYGAK+YHMLGI+LQRSWIVL 
Sbjct: 67  HIGPTELAAYSITFTVLLRFSNGILLGMASALGTLCGQAYGAKQYHMLGIHLQRSWIVLT 126

Query: 123 FCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQCQQKT 182
            C   ++P ++++ P+L  LGQ+D +   + ++A+WLI ++F+F   F  Q FLQ Q K 
Sbjct: 127 GCTICIMPIFIFSGPILLALGQEDHIVRVARVIALWLIAINFTFVPAFTCQIFLQSQSKN 186

Query: 183 LVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWCPLTW 242
            +IA+VS   L L+V  SW+L+  +  G+ GA  +  +++W+       Y   G C  TW
Sbjct: 187 KIIAYVSAVTLGLHVFFSWLLVVHFNFGITGAMTSTLVAFWMPNIVQLLYVTSGGCKDTW 246

Query: 243 TGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCMSING 302
            GF++ AF  LW   KLS ++G M+CLE WY  ILVL+TGNL+NA +A+DAL++C+++N 
Sbjct: 247 RGFTMLAFKDLWPVFKLSLSSGGMVCLELWYNSILVLLTGNLKNAEVAIDALAICINVNA 306

Query: 303 WEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHGKIAL 362
            +MMI + F A V VRV+NELG GN + AKFATIV+V  S  IG+V+  + +   G+I+ 
Sbjct: 307 LQMMIALGFLAAVSVRVSNELGRGNPEGAKFATIVAVFTSLSIGLVLFFVFLFLRGRISY 366

Query: 363 IFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGMLYG---------------------- 422
           IFT+S  V   V  LS LLA +ILLNSVQPVLSG+  G                      
Sbjct: 367 IFTTSEAVAAEVADLSPLLAFSILLNSVQPVLSGVAVGAGWQGYVAYINLACYYLLGIPV 426

Query: 423 ---------------------GTALQTIILIIITIRTDWEKEAEKAQMHVERWSSPKKDE 445
                                G  +QT +L I+T+RTDW+++   +  ++ RW  P+  +
Sbjct: 427 GLVLGYVVGLQVKGVWIGMLFGIFVQTCVLTIMTLRTDWDQQVSTSLKNINRWVVPESRD 486

The following BLAST results are available for this feature:

BLAST of MC08g_new0020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKQ1	2.0e-146	57.88	Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1	[more]
Q1PDX9	4.5e-138	54.27	Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1	[more]
Q9FNC1	1.1e-133	53.08	Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1	[more]
Q8W488	4.7e-103	42.14	Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1	[more]
F4HPH1	8.0e-103	41.65	Protein DETOXIFICATION 22 OS=Arabidopsis thaliana OX=3702 GN=DTX22 PE=2 SV=1	[more]

BLAST of MC08g_new0020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131672.1	1.71e-308	90.98	protein DETOXIFICATION 27-like [Momordica charantia] >XP_022131673.1 protein DET...	[more]
XP_022131671.1	6.91e-264	78.62	protein DETOXIFICATION 27-like [Momordica charantia]	[more]
XP_022997119.1	2.57e-249	74.64	protein DETOXIFICATION 27-like [Cucurbita maxima]	[more]
KAG7029651.1	5.18e-249	75.05	Protein DETOXIFICATION 27 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023545612.1	1.04e-248	74.49	protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC08g_new0020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQC7	8.26e-309	90.98	Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004792 PE=3 SV=1	[more]
A0A6J1BQ61	3.34e-264	78.62	Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004791 PE=3 SV=1	[more]
A0A6J1KAJ6	1.24e-249	74.64	Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111492126 PE=3 SV=1	[more]
A0A6J1HEV9	1.68e-247	74.64	Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111462892 PE=3 SV=1	[more]
A0A6J1BQ65	1.54e-242	71.98	Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004794 PE=3 SV=1	[more]

BLAST of MC08g_new0020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65380.1	1.4e-147	57.88	MATE efflux family protein	[more]
AT5G10420.1	3.2e-139	54.27	MATE efflux family protein	[more]
AT5G44050.1	8.1e-135	53.08	MATE efflux family protein	[more]
AT1G33110.1	3.3e-104	42.14	MATE efflux family protein	[more]
AT1G33090.1	5.7e-104	41.65	MATE efflux family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002528	Multi antimicrobial extrusion protein	TIGRFAM	TIGR00797	TIGR00797	coord: 42..412 e-value: 6.5E-60 score: 201.0
IPR002528	Multi antimicrobial extrusion protein	PFAM	PF01554	MatE	coord: 264..411 e-value: 5.8E-21 score: 74.8 coord: 42..202 e-value: 2.4E-31 score: 108.6
None	No IPR available	PANTHER	PTHR11206	MULTIDRUG RESISTANCE PROTEIN	coord: 394..430 coord: 18..397
None	No IPR available	PANTHER	PTHR11206:SF194	PROTEIN DETOXIFICATION	coord: 394..430 coord: 18..397
IPR045069	Multidrug and toxic compound extrusion family, eukaryotic	CDD	cd13132	MATE_eukaryotic	coord: 32..400 e-value: 1.83562E-128 score: 376.913

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g_new0020.1	MC08g_new0020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990961	xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015297	antiporter activity
molecular_function	GO:0042910	xenobiotic transmembrane transporter activity