MC08g2532 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2532 vs. ExPASy Swiss-Prot
Match: P27061 (Acid phosphatase 1 OS=Solanum lycopersicum OX=4081 GN=APS1 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-58
Identity = 113/257 (43.97%), Postives = 159/257 (61.87%), Query Frame = 0

Query: 32  LLLLLLAFASDSASS----RPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNLITNFTS 91
           L+LL +A  +++ +S    RP+I  +P K L        L C  W++  E NNL    T 
Sbjct: 7   LVLLTVAIGTENLNSHVFPRPLIIEYPEKQL-----RDELKCTTWRFVVETNNLSPWKTI 66

Query: 92  PKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVDDTLLST 151
           P+EC  ++K Y      G  Y +++    + A EYA++V L  DGRD WIF VD+TLLS 
Sbjct: 67  PEECADYVKEYM----VGPGYKMEIDRVSDEAGEYAKSVDLGDDGRDVWIFDVDETLLSN 126

Query: 152 LPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGRDEFTRG 211
           LPYY +  YG E F++ +F++W+    AP +  +  LY+ +  LGFK+ +LTGR E  R 
Sbjct: 127 LPYYSDHRYGLEVFDDVEFDKWVENGTAPALGSSLKLYQEVLKLGFKVFLLTGRSERHRS 186

Query: 212 FTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQWSDVLG 271
            T +NL+ AG+ +W KLI+RG  D GK A  YKSE R  +V EG+R+ GN+GDQWSD+LG
Sbjct: 187 VTVENLMNAGFHDWHKLILRGSDDHGKTATTYKSERRNAMVEEGFRIVGNSGDQWSDLLG 246

Query: 272 LTPAARSFKFPNPMYYV 285
            + + RSFK PNPMYY+
Sbjct: 247 SSMSYRSFKLPNPMYYI 254

BLAST of MC08g2532 vs. ExPASy Swiss-Prot
Match: P15490 (Stem 28 kDa glycoprotein OS=Glycine max OX=3847 GN=VSPA PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.9e-42
Identity = 100/266 (37.59%), Postives = 146/266 (54.89%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDT-NLLCEGWKYSFEVNN 81
           M  L  F+A +L+     A D         MFP +    +   T  + C  W+ + E +N
Sbjct: 3   MKVLVFFVATILVAWQCHAYD---------MFPLRMNTGYGARTPEVKCASWRLAVEAHN 62

Query: 82  LITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGV 141
           +    T P+ECV   K Y     +G +Y  D     + AY YA  +++    +D ++F +
Sbjct: 63  IFGFETIPEECVEATKEYI----HGEQYRSDSKTVNQQAYFYARDLEVHP--KDTFVFSI 122

Query: 142 DDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTG 201
           D T+LS +PYY++ GYG E FN T ++EW+ K +AP +  T   Y  L  LGFKII L+G
Sbjct: 123 DGTVLSNIPYYKKHGYGVEKFNSTLYDEWVNKGNAPALPETLKNYNKLVSLGFKIIFLSG 182

Query: 202 RDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQG-KDAIVYKSEMRAKLVGEGYRLHGNTG 261
           R    +  T+ NL KAGY  WEKLI++   D    +A+ YK+  R KL+ +GY + G  G
Sbjct: 183 RTLDKQAVTEANLKKAGYHTWEKLILKDPQDPSTPNAVSYKTAAREKLIRQGYNIVGIIG 242

Query: 262 DQWSDVL-GLTPAARSFKFPNPMYYV 285
           DQWSD+L G    +R+FK PNP+YY+
Sbjct: 243 DQWSDLLGGHRGESRTFKLPNPLYYI 253

BLAST of MC08g2532 vs. ExPASy Swiss-Prot
Match: P10743 (Stem 31 kDa glycoprotein OS=Glycine max OX=3847 GN=VSPB PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.1e-41
Identity = 92/217 (42.40%), Postives = 132/217 (60.83%), Query Frame = 0

Query: 69  CEGWKYSFEVNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKL 128
           C  ++ + E +N+    T P+ECV   K   DYI NG ++  D     + A+ YA   ++
Sbjct: 43  CASFRLAVEAHNIRAFKTIPEECVEPTK---DYI-NGEQFRSDSKTVNQQAFFYASEREV 102

Query: 129 SSDGRDAWIFGVDDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRIL 188
             +  D +IFG+D+T+LS +PYY++ GYG E FNET ++EW+ K DAP +  T   Y  L
Sbjct: 103 HHN--DIFIFGIDNTVLSNIPYYEKHGYGVEEFNETLYDEWVNKGDAPALPETLKNYNKL 162

Query: 189 QDLGFKIIILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLV 248
             LGFKI+ L+GR       T+ NL KAG+  WE+LI++       +A+ YKS MR  L+
Sbjct: 163 LSLGFKIVFLSGRYLDKMAVTEANLKKAGFHTWEQLILKDPHLITPNALSYKSAMRENLL 222

Query: 249 GEGYRLHGNTGDQWSDVLG-LTPAARSFKFPNPMYYV 285
            +GYR+ G  GDQWSD+LG     +R+FK PNPMYY+
Sbjct: 223 RQGYRIVGIIGDQWSDLLGDHRGESRTFKLPNPMYYI 253

BLAST of MC08g2532 vs. ExPASy Swiss-Prot
Match: P10742 (Stem 31 kDa glycoprotein (Fragment) OS=Glycine max OX=3847 GN=VSP25 PE=2 SV=2)

HSP 1 Score: 168.7 bits (426), Expect = 9.5e-41
Identity = 91/232 (39.22%), Postives = 132/232 (56.90%), Query Frame = 0

Query: 52  MFPRKNLLRFNDDT-NLLCEGWKYSFEVNNLITNFTSPKECVPFLKHYFDYIDNGGRYSV 111
           MFP +    +   T  + C  W+ + E +N+    T P+ECV   K Y     +G +Y  
Sbjct: 21  MFPLRMNTGYGARTPEVKCASWRLAVEAHNIFGFETIPEECVEATKEYI----HGEQYRS 80

Query: 112 DLYYAIEYAYEYAETVKLSSDGRDAWIFGVDDTLLSTLPYYQERGYGSETFNETDFNEWM 171
           D     + AY YA  +++    +D ++F +D T+LS +PYY++ GYG E FN T ++EW+
Sbjct: 81  DSKTVNQQAYFYARDLEVHP--KDTFVFSIDGTVLSNIPYYKKHGYGVEKFNSTLYDEWV 140

Query: 172 AKADAPPVEFTWALYRILQDLGFKIIILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGIS 231
            K +AP +  T   Y  L  LGFKII L+GR    +  T+ NL KAGY  WEKLI++   
Sbjct: 141 NKGNAPALPETLKNYNKLVSLGFKIIFLSGRTLDKQAVTEANLKKAGYHTWEKLILKDPQ 200

Query: 232 DQG-KDAIVYKSEMRAKLVGEGYRLHGNTGDQWSDVL-GLTPAARSFKFPNP 281
           D    +A+ YK+  R KL+ +GY + G  GDQWSD+L G    +R+FK PNP
Sbjct: 201 DPSTPNAVSYKTAAREKLIRQGYNIVGIIGDQWSDLLGGHRGESRTFKLPNP 246

BLAST of MC08g2532 vs. ExPASy Swiss-Prot
Match: O82122 (Vegetative storage protein 2 OS=Arabidopsis thaliana OX=3702 GN=VSP2 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.9e-37
Identity = 98/269 (36.43%), Postives = 146/269 (54.28%), Query Frame = 0

Query: 29  LALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLL-----------CEGWKYSFE 88
           L+L LLLLLA A+ SAS   +I +   K +  F +   LL           C  W    E
Sbjct: 4   LSLSLLLLLA-ATVSASVPGLIELVDSKTI--FGNVAELLEKEKLSINYANCRSWHLGVE 63

Query: 89  VNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWI 148
            +N+I   T P  C  +++   DY+    +Y  D     + AY YA+ + L +D  + WI
Sbjct: 64  TSNIIDFDTVPANCKDYVE---DYLITSKQYQYDSKTVCKEAYFYAKGLALKNDTVNVWI 123

Query: 149 FGVDDTLLSTLPYYQERGYGSETFNETDFNEWMAK-ADAPPVEFTWALYRILQDLGFKII 208
           F +DDTLLS++PYY + GYG+E  +   +  W+   A  P +     LY+ + +LG + I
Sbjct: 124 FDLDDTLLSSIPYYAKYGYGTEKTDPGAYWLWLGTGASTPGLPEALHLYQNIIELGIEPI 183

Query: 209 ILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHG 268
           IL+ R +  +  T  NL  AG + W+ LI++  +      +VYKS++R  LV +GY + G
Sbjct: 184 ILSDRWKLWKNVTLDNLEAAGVTYWKHLILKP-NGSNLRQVVYKSKVRKSLVKKGYNIVG 243

Query: 269 NTGDQWSDVLGLTPAARSFKFPNPMYYVP 286
           N GDQW+D++  TP  R FK PNP+YYVP
Sbjct: 244 NIGDQWADLVEDTP-GRVFKLPNPLYYVP 264

BLAST of MC08g2532 vs. NCBI nr
Match: XP_022154690.1 (acid phosphatase 1-like [Momordica charantia])

HSP 1 Score: 544 bits (1401), Expect = 5.63e-195
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL
Sbjct: 1   MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVD 141
           ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVD
Sbjct: 61  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVD 120

Query: 142 DTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGR 201
           DTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGR
Sbjct: 121 DTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGR 180

Query: 202 DEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQ 261
           DEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQ
Sbjct: 181 DEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQ 240

Query: 262 WSDVLGLTPAARSFKFPNPMYYVP 285
           WSDVLGLTPAARSFKFPNPMYYVP
Sbjct: 241 WSDVLGLTPAARSFKFPNPMYYVP 264

BLAST of MC08g2532 vs. NCBI nr
Match: XP_023546696.1 (acid phosphatase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271 bits (692), Expect = 3.56e-87
Identity = 137/265 (51.70%), Postives = 179/265 (67.55%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MAS  S L+LLLLLLL   + + SS   +R++PR++++R   D    CE WK++ EVN  
Sbjct: 1   MASPTSILSLLLLLLLLLVTAAVSSAAAVRIYPREHVVRAEGDPR--CESWKFAVEVNAA 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSD-GRDAWIFGV 141
            +    P+ C+ F++ YF    NG RY  D      Y+  +A +VKL  D GRDAW+F V
Sbjct: 61  GSWKAVPRPCIEFVREYF----NGHRYLSDSRAVANYSLTFAYSVKLGKDHGRDAWVFDV 120

Query: 142 DDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTG 201
           D+TLLS LPYY+  G+GS+ FN+T FNEW+    APP+  +  LY+ L+ LGFKI +LTG
Sbjct: 121 DETLLSNLPYYRANGFGSKPFNDTSFNEWVNTGSAPPLPMSLRLYKKLKSLGFKIFLLTG 180

Query: 202 RDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGD 261
           R E  R  TQQNLL+AGYS WEKLI+RG  D+GK A VYKSE RA+LV +GY + GN+GD
Sbjct: 181 RGESQRAVTQQNLLQAGYSGWEKLILRGPDDEGKKATVYKSEKRAELVKQGYIIQGNSGD 240

Query: 262 QWSDVLGLTPAARSFKFPNPMYYVP 285
           QWSD++G   A RSFK PN MYY+P
Sbjct: 241 QWSDLMGFALAKRSFKLPNSMYYIP 259

BLAST of MC08g2532 vs. NCBI nr
Match: XP_023547254.1 (acid phosphatase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268 bits (685), Expect = 4.10e-86
Identity = 136/265 (51.32%), Postives = 178/265 (67.17%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MAS  S L+LLL LLL   + + SS   +R++PR++++R   D    CE WK++ EVN  
Sbjct: 1   MASPTSILSLLLPLLLLLVTAAVSSAAAVRIYPREHVVRAEGDPR--CESWKFAVEVNAA 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSD-GRDAWIFGV 141
            +    P+ C+ F++ YF    NG RY  D      Y+  +A +VKL  D GRDAW+F V
Sbjct: 61  GSWKAVPRPCIEFVREYF----NGHRYLSDSRAVANYSLTFAYSVKLGKDHGRDAWVFDV 120

Query: 142 DDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTG 201
           D+TLLS LPYY+  G+GS+ FN+T FNEW+    APP+  +  LY+ L+ LGFKI +LTG
Sbjct: 121 DETLLSNLPYYRANGFGSKPFNDTSFNEWVNTGSAPPLPMSLRLYKKLKSLGFKIFLLTG 180

Query: 202 RDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGD 261
           R E  R  TQQNLL+AGYS WEKLI+RG  D+GK A VYKSE RA+LV +GY + GN+GD
Sbjct: 181 RGESQRAVTQQNLLQAGYSGWEKLILRGPDDEGKKATVYKSEKRAELVKQGYIIQGNSGD 240

Query: 262 QWSDVLGLTPAARSFKFPNPMYYVP 285
           QWSD++G   A RSFK PN MYY+P
Sbjct: 241 QWSDLMGFALAKRSFKLPNSMYYIP 259

BLAST of MC08g2532 vs. NCBI nr
Match: XP_038890795.1 (acid phosphatase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 268 bits (684), Expect = 6.20e-86
Identity = 135/270 (50.00%), Postives = 183/270 (67.78%), Query Frame = 0

Query: 18  SPPTMASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFE 77
           SPP++ SL     LL  ++ A  S + SS  ++RM+PRK+++R   D    CE WK++ E
Sbjct: 3   SPPSILSL-----LLHFVITATVSSADSSGAMVRMYPRKHVVRAEGDPR--CESWKFAVE 62

Query: 78  VNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLS--SDGRDA 137
           VN      + P+ C+ F++ YF    NGGRY  D    ++Y+  +A +V ++    GRDA
Sbjct: 63  VNAAGPWKSVPRPCIGFVREYF----NGGRYLSDSKVVVKYSLTFANSVNVAVKDSGRDA 122

Query: 138 WIFGVDDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKI 197
           W+F VD+TLLS LPYY+  GYGS+ +N+T FNEW+ K  APP+  +  LY+ LQ+LGFKI
Sbjct: 123 WVFDVDETLLSNLPYYKVNGYGSKPYNDTSFNEWVNKGLAPPLPMSLRLYKKLQNLGFKI 182

Query: 198 IILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLH 257
            +LTGR E  R  T++NLLKAGYS WEKLI+RG +D+GK A VYKSE  A+LV EGY + 
Sbjct: 183 FLLTGRGESQRNITEENLLKAGYSGWEKLILRGDADEGKKATVYKSEKTAELVKEGYIIQ 242

Query: 258 GNTGDQWSDVLGLTPAARSFKFPNPMYYVP 285
           G+ GDQWSD++G   A RSFK PNPMYY+P
Sbjct: 243 GSFGDQWSDLMGFPLANRSFKLPNPMYYIP 261

BLAST of MC08g2532 vs. NCBI nr
Match: XP_022999520.1 (acid phosphatase 1-like [Cucurbita maxima])

HSP 1 Score: 265 bits (678), Expect = 4.87e-85
Identity = 139/269 (51.67%), Postives = 183/269 (68.03%), Query Frame = 0

Query: 18  SPPTMASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFE 77
           + PT  S+ S L LLLLLLL  A+ SA++   IR++PR++++R   D    CE WK++ E
Sbjct: 2   ASPT--SILSLLLLLLLLLLVTAAVSAAAA--IRIYPREHVVRAEGDPR--CESWKFAVE 61

Query: 78  VNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSD-GRDAW 137
           VN   +  T P+ C+ F++ YF    NG RY  D      Y+  +A +VK+  D GRDAW
Sbjct: 62  VNAAGSWKTVPRPCIEFVREYF----NGHRYLSDSRAVANYSLTFANSVKVGKDHGRDAW 121

Query: 138 IFGVDDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKII 197
           +F VD+TLLS LPYY+  G+GS+ FN+T FNEW+    APP+  +  LY+ L+ LGFKI 
Sbjct: 122 VFDVDETLLSNLPYYRANGFGSKPFNDTSFNEWVNTGSAPPLPMSLRLYKKLKSLGFKIF 181

Query: 198 ILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHG 257
           +LTGR E  R  TQQNLL+AGYS WEKLI+RG  D+GK A VYKSE RA+LV +GY + G
Sbjct: 182 LLTGRGESQRVVTQQNLLQAGYSGWEKLILRGPYDEGKKATVYKSEKRAELVKQGYIIQG 241

Query: 258 NTGDQWSDVLGLTPAARSFKFPNPMYYVP 285
           N+GDQWSD++G   A RSFK PN MYY+P
Sbjct: 242 NSGDQWSDLMGFALAKRSFKLPNSMYYIP 260

BLAST of MC08g2532 vs. ExPASy TrEMBL
Match: A0A6J1DKC3 (acid phosphatase 1-like OS=Momordica charantia OX=3673 GN=LOC111021887 PE=4 SV=1)

HSP 1 Score: 544 bits (1401), Expect = 2.72e-195
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL
Sbjct: 1   MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVD 141
           ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVD
Sbjct: 61  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVD 120

Query: 142 DTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGR 201
           DTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGR
Sbjct: 121 DTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGR 180

Query: 202 DEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQ 261
           DEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQ
Sbjct: 181 DEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQ 240

Query: 262 WSDVLGLTPAARSFKFPNPMYYVP 285
           WSDVLGLTPAARSFKFPNPMYYVP
Sbjct: 241 WSDVLGLTPAARSFKFPNPMYYVP 264

BLAST of MC08g2532 vs. ExPASy TrEMBL
Match: A0A6J1KFL8 (acid phosphatase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493856 PE=4 SV=1)

HSP 1 Score: 265 bits (678), Expect = 2.36e-85
Identity = 139/269 (51.67%), Postives = 183/269 (68.03%), Query Frame = 0

Query: 18  SPPTMASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFE 77
           + PT  S+ S L LLLLLLL  A+ SA++   IR++PR++++R   D    CE WK++ E
Sbjct: 2   ASPT--SILSLLLLLLLLLLVTAAVSAAAA--IRIYPREHVVRAEGDPR--CESWKFAVE 61

Query: 78  VNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSD-GRDAW 137
           VN   +  T P+ C+ F++ YF    NG RY  D      Y+  +A +VK+  D GRDAW
Sbjct: 62  VNAAGSWKTVPRPCIEFVREYF----NGHRYLSDSRAVANYSLTFANSVKVGKDHGRDAW 121

Query: 138 IFGVDDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKII 197
           +F VD+TLLS LPYY+  G+GS+ FN+T FNEW+    APP+  +  LY+ L+ LGFKI 
Sbjct: 122 VFDVDETLLSNLPYYRANGFGSKPFNDTSFNEWVNTGSAPPLPMSLRLYKKLKSLGFKIF 181

Query: 198 ILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHG 257
           +LTGR E  R  TQQNLL+AGYS WEKLI+RG  D+GK A VYKSE RA+LV +GY + G
Sbjct: 182 LLTGRGESQRVVTQQNLLQAGYSGWEKLILRGPYDEGKKATVYKSEKRAELVKQGYIIQG 241

Query: 258 NTGDQWSDVLGLTPAARSFKFPNPMYYVP 285
           N+GDQWSD++G   A RSFK PN MYY+P
Sbjct: 242 NSGDQWSDLMGFALAKRSFKLPNSMYYIP 260

BLAST of MC08g2532 vs. ExPASy TrEMBL
Match: A0A6J1KB36 (acid phosphatase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493858 PE=4 SV=1)

HSP 1 Score: 263 bits (673), Expect = 1.07e-84
Identity = 137/264 (51.89%), Postives = 179/264 (67.80%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MAS  S L+LLLL      +   SS  +IRM+PR++L+R + D    CE WK+S EVN  
Sbjct: 1   MASPTSILSLLLL------AAVVSSAEVIRMYPREHLVRLDSDPE--CESWKFSVEVNAA 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSS-DGRDAWIFGV 141
            +  + P+ CV F++ YF    N GRY  D      Y+  +A +V ++  DG DAW+F V
Sbjct: 61  GSWSSIPRPCVSFVQDYF----NTGRYLSDSISVASYSENFALSVNVADGDGMDAWVFDV 120

Query: 142 DDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTG 201
           D+TLLS LPYY++ G+GSE +NET FNEW+ K  AP +  +  LY+ ++ LGFKI ILTG
Sbjct: 121 DETLLSNLPYYKDNGFGSEPYNETSFNEWVYKGLAPVLPASLTLYKRVKKLGFKIFILTG 180

Query: 202 RDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGD 261
           R EF R  T+QNL+ AGYS WEKLI+RG  D+GK AIVYKSE R +LV +GYR+ G++GD
Sbjct: 181 RAEFQRAVTEQNLIDAGYSGWEKLILRGREDEGKKAIVYKSEKRDELVKQGYRIQGSSGD 240

Query: 262 QWSDVLGLTPAARSFKFPNPMYYV 284
           QWSD++G   A RSFK PNPMYYV
Sbjct: 241 QWSDLMGFALAKRSFKLPNPMYYV 252

BLAST of MC08g2532 vs. ExPASy TrEMBL
Match: A0A6J1G2F8 (acid phosphatase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450211 PE=4 SV=1)

HSP 1 Score: 263 bits (671), Expect = 2.15e-84
Identity = 136/264 (51.52%), Postives = 178/264 (67.42%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MAS  S L+LLLL      +   SS  +IRM+PR++L+R + D    CE WK+S EVN  
Sbjct: 1   MASPTSILSLLLL------AAVVSSAEVIRMYPREHLVRLDSDPE--CESWKFSVEVNAA 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSS-DGRDAWIFGV 141
            +  + P+ CV F++ YF    N GRY  D      Y+  +A +V ++  DG DAW+F V
Sbjct: 61  GSWSSIPRPCVSFVQDYF----NTGRYLSDSISVASYSENFALSVNVADGDGMDAWVFDV 120

Query: 142 DDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTG 201
           D+TLLS LPYY++ G+GSE +NET FNEW+ K  AP +  +  LY+ ++ LGFKI ILTG
Sbjct: 121 DETLLSNLPYYKDNGFGSEPYNETSFNEWVYKGLAPVLPASLTLYKRVKKLGFKIFILTG 180

Query: 202 RDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGD 261
           R EF R  T+QNL+ AGYS WEKLI+RG  D+GK A +YKSE R +LV +GYR+ GN+GD
Sbjct: 181 RAEFQRAITEQNLIDAGYSGWEKLILRGHQDEGKKATLYKSEKRDELVKQGYRIQGNSGD 240

Query: 262 QWSDVLGLTPAARSFKFPNPMYYV 284
           QWSD++G   A RSFK PNPMYYV
Sbjct: 241 QWSDLMGFALAKRSFKLPNPMYYV 252

BLAST of MC08g2532 vs. ExPASy TrEMBL
Match: A0A6J1G2J9 (acid phosphatase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450213 PE=4 SV=1)

HSP 1 Score: 263 bits (671), Expect = 2.22e-84
Identity = 135/265 (50.94%), Postives = 179/265 (67.55%), Query Frame = 0

Query: 22  MASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNL 81
           MAS  S L+LLLL+  A +S +A     +R++PR++++R   D    CE WK++ EVN  
Sbjct: 1   MASPTSILSLLLLVTAAVSSAAA-----VRIYPREHVVRAEGDPR--CESWKFAVEVNAA 60

Query: 82  ITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSD-GRDAWIFGV 141
            +  T P+ C+ F++ YF    NG RY  D      Y+  +A +VKL  D GRDAW+F V
Sbjct: 61  GSWKTVPRPCIEFVREYF----NGHRYLSDSRAVANYSLTFAYSVKLGKDHGRDAWVFDV 120

Query: 142 DDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTG 201
           D+TLLS LPYY+  G+GS+ FN+T FNEW+    APP+  +  LY+ L+ LGFKI +LTG
Sbjct: 121 DETLLSNLPYYRANGFGSKPFNDTSFNEWVNTGSAPPLPMSLRLYKKLKSLGFKIFLLTG 180

Query: 202 RDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGD 261
           R E  R  TQ+NLL+AGYS WEKLI+RG  D+GK A VYKSE RA+LV +GY + GN+GD
Sbjct: 181 RGESQRVVTQKNLLRAGYSGWEKLILRGTDDEGKKATVYKSEKRAELVKQGYIIQGNSGD 240

Query: 262 QWSDVLGLTPAARSFKFPNPMYYVP 285
           QWSD++G   A RSFK PN MYY+P
Sbjct: 241 QWSDLMGFALAKRSFKLPNSMYYIP 254

BLAST of MC08g2532 vs. TAIR 10
Match: AT4G25150.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-61
Identity = 119/261 (45.59%), Postives = 165/261 (63.22%), Query Frame = 0

Query: 29  LALLLLLLLAFASDSASS----RPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNLITN 88
           L L  L+ LAF+++++SS    RP+I     KN+   ND+ NL C  W+++ E NNL   
Sbjct: 7   LILFSLIPLAFSNENSSSYLIARPLIFETQLKNI---NDNVNLHCTSWRFAAETNNLAPW 66

Query: 89  FTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVDDTL 148
            T P EC  ++K Y      G  Y VD+    E A  YA + + + DG+D WIF +D+TL
Sbjct: 67  KTIPAECADYVKDYL----MGEGYVVDVERVSEEAKVYASSFESNGDGKDIWIFDIDETL 126

Query: 149 LSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGRDEF 208
           LS LPYY E G G E F+ + F+ W+ K  AP +  +  LY+ +  LG+K+I+LTGR E 
Sbjct: 127 LSNLPYYMEHGCGLEVFDHSKFDMWVEKGIAPAIAPSLKLYQKVIHLGYKVILLTGRREN 186

Query: 209 TRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQWSD 268
            R  T +NL  AG+ NW+KLI+R + D+ K A +YKSE R ++V EGYR+ GN+GDQWSD
Sbjct: 187 HRVITVENLRNAGFHNWDKLILRSLDDRNKTATMYKSEKREEMVKEGYRIRGNSGDQWSD 246

Query: 269 VLGLTPAARSFKFPNPMYYVP 286
           +LG   + RSFK PNPMYY+P
Sbjct: 247 LLGSAMSERSFKLPNPMYYIP 260

BLAST of MC08g2532 vs. TAIR 10
Match: AT4G29270.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 233.4 bits (594), Expect = 2.2e-61
Identity = 115/262 (43.89%), Postives = 164/262 (62.60%), Query Frame = 0

Query: 23  ASLKSFLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNLI 82
           +S+  F+  L  +L+  A  S ++  I    PR ++  +       CE W+ + E NN+ 
Sbjct: 7   SSITFFIVALFTVLINPAISSRAASFI--KLPRSSIASY-------CESWRLAAETNNVG 66

Query: 83  TNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVDD 142
                P +C  ++K+Y     NGG++  D      YA +YA+TVK+  DG+DAW+F +D+
Sbjct: 67  PWKVIPSQCENYIKNYI----NGGQFDKDYDVVASYAIDYAKTVKVGGDGKDAWVFDIDE 126

Query: 143 TLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGRD 202
           TLLS + YY+  GYGSE ++   +NE + K   P  + +  LY+ L+ LGF II+LTGRD
Sbjct: 127 TLLSNIEYYKANGYGSEPYDSIKYNEVVEKGKDPGYDASLRLYKALKKLGFTIILLTGRD 186

Query: 203 EFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQW 262
           E  R  T++NL  AGY  W +L++RG +DQGK A  YKSE R+++V EGY +HGNTGDQW
Sbjct: 187 EGHRSVTEKNLRDAGYFGWNRLLLRGQNDQGKTATQYKSEQRSQVVKEGYTIHGNTGDQW 246

Query: 263 SDVLGLTPAARSFKFPNPMYYV 285
           SD+LG   A+RSFK PNPMYYV
Sbjct: 247 SDLLGFAVASRSFKVPNPMYYV 255

BLAST of MC08g2532 vs. TAIR 10
Match: AT4G29260.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 229.6 bits (584), Expect = 3.2e-60
Identity = 103/222 (46.40%), Postives = 147/222 (66.22%), Query Frame = 0

Query: 64  DTNLLCEGWKYSFEVNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYA 123
           D +  C+ W+ + E NN+ T    P  CV  +  Y     NG ++  D    ++YA  +A
Sbjct: 38  DGSRYCDSWRLAAETNNVGTWDLIPSICVDSVAEYL----NGDQFLSDYSVIVDYALAFA 97

Query: 124 ETVKLSSDGRDAWIFGVDDTLLSTLPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWA 183
           ++V++S DG+D WIF +D+TLL+ + YY+  GYGSE +++  F+EW+ +  AP  + +  
Sbjct: 98  KSVEISGDGKDVWIFDIDETLLTNIDYYKAHGYGSEPYDDNKFSEWVEQGTAPAFDASLR 157

Query: 184 LYRILQDLGFKIIILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEM 243
           LY  L+ LGF II+LTGRDE  R  T+ NL  AGYS WE+L++RG +DQGK A  YKSE 
Sbjct: 158 LYNALKKLGFTIILLTGRDEHQRTSTETNLRDAGYSGWERLLLRGPNDQGKSATNYKSEQ 217

Query: 244 RAKLVGEGYRLHGNTGDQWSDVLGLTPAARSFKFPNPMYYVP 286
           R+KL+ EG+++ GN+GDQWSD+ G   A RSFK PNPMYY+P
Sbjct: 218 RSKLIEEGFKIRGNSGDQWSDLQGFAVADRSFKVPNPMYYIP 255

BLAST of MC08g2532 vs. TAIR 10
Match: AT5G51260.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 222.2 bits (565), Expect = 5.2e-58
Identity = 112/258 (43.41%), Postives = 162/258 (62.79%), Query Frame = 0

Query: 28  FLALLLLLLLAFASDSASSRPIIRMFPRKNLLRFNDDTNLLCEGWKYSFEVNNLITNFTS 87
           FL ++ L   AF SDS    P       ++     +D NL C  W+++ E+NNL    T 
Sbjct: 7   FLVVVSLFTSAF-SDSILEYP--SEIESRHKKAAEEDVNLHCTTWRFAAEMNNLAPWKTI 66

Query: 88  PKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETVKLSSDGRDAWIFGVDDTLLST 147
           P EC  ++K   DY+   G Y  DL    E A  +A +++ S DG+D WIF +D+TLLS 
Sbjct: 67  PVECADYVK---DYVMGKG-YLTDLERVSEEALIFARSIEFSGDGKDIWIFDIDETLLSN 126

Query: 148 LPYYQERGYGSETFNETDFNEWMAKADAPPVEFTWALYRILQDLGFKIIILTGRDEFTRG 207
           LPYY + G+G E F+ ++F++W+ +  AP +  +  LY+ + DLG+K+ +LTGR E  R 
Sbjct: 127 LPYYIDHGFGLELFDHSEFDKWVERGVAPAIAPSLKLYQRVLDLGYKVFLLTGRKESHRL 186

Query: 208 FTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMRAKLVGEGYRLHGNTGDQWSDVLG 267
            T +NL+ AG+ NW+KLI+R   +Q K A +YKSE R ++V EGYR+ GN+GDQWSD+LG
Sbjct: 187 VTVENLINAGFQNWDKLILRSPEEQHKMATLYKSEKRDEMVKEGYRIRGNSGDQWSDLLG 246

Query: 268 LTPAARSFKFPNPMYYVP 286
            + + RSFK  NPMYY+P
Sbjct: 247 TSMSQRSFKLANPMYYIP 257

BLAST of MC08g2532 vs. TAIR 10
Match: AT1G04040.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-44
Identity = 87/220 (39.55%), Postives = 125/220 (56.82%), Query Frame = 0

Query: 69  CEGWKYSFEVNNLITNFTSPKECVPFLKHYFDYIDNGGRYSVDLYYAIEYAYEYAETV-- 128
           CE W+ + EV+N+      P+ECV  +K Y        +Y  D+   ++    +  ++  
Sbjct: 55  CESWRINVEVHNIRKFDVVPQECVSHIKDYM----TSSQYKDDVARTVDEVILHFGSMCC 114

Query: 129 -KLSSDGRDAWIFGVDDTLLSTLPYYQERG-YGSETFNETDFNEWMAKADAPPVEFTWAL 188
            K   DG DAWIF +DDTLLST+PY+++ G +G E  N T F +W+ K  AP V     L
Sbjct: 115 SKSKCDGMDAWIFDIDDTLLSTIPYHKKNGFFGGEKLNSTKFEDWIQKKKAPAVPHMKKL 174

Query: 189 YRILQDLGFKIIILTGRDEFTRGFTQQNLLKAGYSNWEKLIMRGISDQGKDAIVYKSEMR 248
           Y  +++ G KI +++ R E+ R  T  NL++AGY  W  L++RG+ DQ K+   YKSE R
Sbjct: 175 YHDIRERGIKIFLISSRKEYLRSATVDNLIQAGYYGWSNLMLRGLEDQQKEVKQYKSEKR 234

Query: 249 AKLVGEGYRLHGNTGDQWSDVLGLTPAARSFKFPNPMYYV 285
             L+  GYR+ G  GDQWS   G     R+FK PN +YYV
Sbjct: 235 KWLMSLGYRVWGVMGDQWSSFAGCPLPRRTFKLPNSIYYV 270