MC08g2519 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2519
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationMC08: 33969556 .. 33973069 (-)
RNA-Seq ExpressionMC08g2519
SyntenyMC08g2519
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATTCGGGCCGAAGATGATCCGGCTCCGAAATTTCTCGCTCTCAATCGTATCGACCTTTCGAGCTCAGATATTCATCAATCGGTTTCGTTACTCAAACAGGTAATCTTCAATTTGATAACGAAAGTAGTTGATATCTTCGATTTCGATTTTTGTTTCTACACAGAAATTGAAACCGAACTTTGTATACTGATTGAAGTAGCTCTTGATTCGTTCAGGCTTGTTTGGATACCGGATTCTTCTATGCTGTGAATCACGGAATCGCGGAGGAATTTATGGATGAGGTTTTTGCTCAGAGCAAAAAGCTTTTTAGTCTGCCGCTAAGCAATAAAATGAAGCTTCTGAGAAACGAGAAGCATAGAGGCTACACTCCTACACTCAAGTATGTTTTCTGCAATTCGCAATTCGTCTATGAGCACTGCAAAGAGAAATTAAAATTGCTGCACAGTTTTCTTCCTCAAATGCTTGGAATTTGATATTCTCAATTGTATATTGTTGTCTATTTAAGTAGGAGATTACAAGGAGGGGTATTACATAGGAATCGAGGAGAAGATTCCTGGAGATGATCCAGATGGAGAAAGGCCATTTTATGGTCCAAATATTTGGCCTGCAGAAGGTATCCCCTCAAAAACAAAGCAAAAAAAATGTACATGTATATTGGCTTTCGCTCAATATGTTTTTTTTGGTCTTTTAGATTTGTTACCTGGCTGGAGGGAGATCATGGAGAAGTTTCATACACAAGCACTGTATGTTATCGTTCTGGTGATTAGACAAATTTATTTAAATAGAGTTGTTATTCCAGTCTGCAAAGCGTCCGATCAATGGTAATAAGAGTTCTGCCAATCCCTCGTCTTGATTTATTAATATTTAGTTTAGGCACCCATATTTGATAAAAAAAAGTTGTGAGTTATAATCCCCACTCACATATTCAGCGTTTTCTTCTTGGCATAACCCAATAGATCAATTAAAAAATATTTATATCTTGATTTCTTTTATAAAAAATGTGAAATCAGAGAGGTTGCTAGAGCAATCTCAAGGATCATTGCTCTTGCGCTTGATCTTGAGATTGATTTTTTTGACAAGCCAGAAATGCTTGGTGAGCCAATTGCAGTCTTGCGCCTACTACACTATGAAGGTGTGAAACGAACATTTTTCTTCACTCTTGGTTCTGTGGCATATTATGGCTATATATAAATCATAAACTGTCCTGTTTCTGCATCAGCCCAAATTTCTGAACCCTCAAAAGGAATATTTGGAGCTGGAGCTCATTCAGACTATGGTTTAATCACACTCTTGGCGACAGACGAGAACCTCGGTCTCCAAGTAAGCAATTCCATTGTGTTCTCATCATCAGCATGCCATATGATTTGATTAGCCATTGAAAGTATTTGCAATTAGAACAGATTTGCAAGGACAAAGATGCTAAACCTCAGATATGGGAATATGTAGCACCACTCAAAGGGTGAGTTCTATGGAATTATTATGAACTAGTTGATCATATGCTTGATGATATCATTTTCATTTAGTATTCTTGCATGTATAAAGTTTGAAAAGACGATCATGAAAATGGCACAGAGTTGTTCAGTGTGCATCATAGATTATACTCGTGTTCTAAAATTCCACTCCAGAGTTTAAAAAATGACAAAAATACAGAGAAGGAAGCTATTTTTAGATGAGCAAACAAAAAATATTGTGTTTTTCTGGCTTCATGATTTCGGTCTGTATGGTATTTAACAGGGCGTTTGTGGTGAACCTTGGAGACATGCTGGAGCGATGGAGCAACTGCATATTCAAGTAAGATACAAAAATTTTCAACCTTTCATTTGTCTATTTGTCATTAACATTTAATCCAGATGTATGTTGAAGCATGATGAACTTTCAAATGTGATATTCTCTCAGATTTTATGAACTGATCATATGCTTTAAATTGTTTTTCAATATGCAATAATGATGTATTTATTTGCCTTCCCTAATACCTTCAAGAATCTTGAATGAGACTATATGAGTTGTTGCTTTTATTTGCATTCAATGTTACAATGAAAGTTGTAAATATTTAATTAGTCATACTTCACTTGAGCATCTTTTAAATATTTGTTTTTGGATGTAACATAACCTAACTCTTTTTCTTCTTATGTTCGATCTCAGGTCCACATTACACAGAGTTCTGGTGAATGGTCAAGATAGATATTCTGTAAGCCTTGCAAAACTTTGCAAAACTATTTAGTTCATCAATAACGGAGTCGTCTCCTCTCCTCAAAGAATAATACTTAGATACCTATCAATCAGCATCCATCTTTATGTTAACTAGGAATAGCAAAAAAACCCACATGACCCGTCGAGTCGGAAATCCCCGATTAAACTAGTGACGGGGTCAAATCGGGGATCCTAATCGGGGTCCCCGACGGGGATGGGGAGATCTCCTCACCTCGTCCCCGCTCTGTTTAATATTTTATTAATTTTTATTTTATATATATAAAATAATATTAATATATATTATTTTTTATAAATACTTTTTTATTTTTTTAAATATTGTAGTTGTATTTAATTAAATTTTACGTTATTCCATAAAAAAATGTTATATTTTCTAAAGAATTAAAATATTTATAAATTTTTTTAAAAAAAGATAAAATACAAATAAAAAAAAATCGGGGAATCCCAATCCCGTCCCCGCTCCGTTGCCATCCCTAATACCAACCATCGATAAAAATTTGATAGTTTTTTAGTTTTTTTAAGAGAAATCTGTTTCTCAATCTTATCTATCAAATAAAAATTTAATGGTAAATAGCAAAAAAATGGGAGCTGATTGGTCAAGCGATTTTCAAATATACAAAAAAGAAGAGAAAAACCATTGTCTATTAGTAGCAACGGTGCATGTTACATGAAATTCCTCTGAATATTGATTGTAAGATTTTTATTTTTTTTATGAGAGCAGATTGCATATTTTGTGGAACCTAGTCATGATTGTCTTGTGGAATGTTTGCCAACTTGCAAGTCAGAGAAAAACCCTCCCAAGTAAGTAGATATTTTGTGTAAAATTTAGTTTACCTCCTTTTGTTTTGTTCTTCATTTCATTCCATTGTTTCTGTTTCATGGAGGCATAATAATATTCCTCTTAATCTCTTTCTTTAATTTATAATTTGAGAATTTTTACTAAAAAAATATATACTCCTCTGTTGCAAACCAACTCGATCCTCCCTACGCTCAGTTTCTATTTTAAATTTGGCTTAGTTTCCTTCCTCTTCTTTCCTTTAATTTTATCTATTAAAATATTAAATTTATTCAAACTTATCAAGCTTTGAAATTTTACAGTTATGTTTACACATCTTAGTCACCTCTCCCTTCAAGATTTATTGCCTTGAACTACAACATTTTCGTAAATGAACATTGAAAATAATATTCTAAGTTTTCTTTCTTTTTCTTATGACAATATTCTATTGTTAGGTTTCCTCCGATTCTATGCAAGACATATTTAAGTCAGCGTTACCAAGAAACTCATGTTGATCTAAATACA

mRNA sequence

ATGGAGATTCGGGCCGAAGATGATCCGGCTCCGAAATTTCTCGCTCTCAATCGTATCGACCTTTCGAGCTCAGATATTCATCAATCGGTTTCGTTACTCAAACAGGCTTGTTTGGATACCGGATTCTTCTATGCTGTGAATCACGGAATCGCGGAGGAATTTATGGATGAGGTTTTTGCTCAGAGCAAAAAGCTTTTTAGTCTGCCGCTAAGCAATAAAATGAAGCTTCTGAGAAACGAGAAGCATAGAGGCTACACTCCTACACTCAAGTATGTTTTCTGCAATTCGCAATTCGTCTATGAGCACTGCAAAGAGAAATTAAAATTGCTGCACAGAGATTACAAGGAGGGGTATTACATAGGAATCGAGGAGAAGATTCCTGGAGATGATCCAGATGGAGAAAGGCCATTTTATGGTCCAAATATTTGGCCTGCAGAAGATTTGTTACCTGGCTGGAGGGAGATCATGGAGAAGTTTCATACACAAGCACTAGAGGTTGCTAGAGCAATCTCAAGGATCATTGCTCTTGCGCTTGATCTTGAGATTGATTTTTTTGACAAGCCAGAAATGCTTGGTGAGCCAATTGCAGTCTTGCGCCTACTACACTATGAAGCCCAAATTTCTGAACCCTCAAAAGGAATATTTGGAGCTGGAGCTCATTCAGACTATGGTTTAATCACACTCTTGGCGACAGACGAGAACCTCGGTCTCCAAATTTGCAAGGACAAAGATGCTAAACCTCAGATATGGGAATATGTAGCACCACTCAAAGGGGCGTTTGTGGTGAACCTTGGAGACATGCTGGAGCGATGGAGCAACTGCATATTCAAGTCCACATTACACAGAGTTCTGGTGAATGGTCAAGATAGATATTCTATTGCATATTTTGTGGAACCTAGTCATGATTGTCTTGTGGAATGTTTGCCAACTTGCAAGTCAGAGAAAAACCCTCCCAAGTTTCCTCCGATTCTATGCAAGACATATTTAAGTCAGCGTTACCAAGAAACTCATGTTGATCTAAATACA

Coding sequence (CDS)

ATGGAGATTCGGGCCGAAGATGATCCGGCTCCGAAATTTCTCGCTCTCAATCGTATCGACCTTTCGAGCTCAGATATTCATCAATCGGTTTCGTTACTCAAACAGGCTTGTTTGGATACCGGATTCTTCTATGCTGTGAATCACGGAATCGCGGAGGAATTTATGGATGAGGTTTTTGCTCAGAGCAAAAAGCTTTTTAGTCTGCCGCTAAGCAATAAAATGAAGCTTCTGAGAAACGAGAAGCATAGAGGCTACACTCCTACACTCAAGTATGTTTTCTGCAATTCGCAATTCGTCTATGAGCACTGCAAAGAGAAATTAAAATTGCTGCACAGAGATTACAAGGAGGGGTATTACATAGGAATCGAGGAGAAGATTCCTGGAGATGATCCAGATGGAGAAAGGCCATTTTATGGTCCAAATATTTGGCCTGCAGAAGATTTGTTACCTGGCTGGAGGGAGATCATGGAGAAGTTTCATACACAAGCACTAGAGGTTGCTAGAGCAATCTCAAGGATCATTGCTCTTGCGCTTGATCTTGAGATTGATTTTTTTGACAAGCCAGAAATGCTTGGTGAGCCAATTGCAGTCTTGCGCCTACTACACTATGAAGCCCAAATTTCTGAACCCTCAAAAGGAATATTTGGAGCTGGAGCTCATTCAGACTATGGTTTAATCACACTCTTGGCGACAGACGAGAACCTCGGTCTCCAAATTTGCAAGGACAAAGATGCTAAACCTCAGATATGGGAATATGTAGCACCACTCAAAGGGGCGTTTGTGGTGAACCTTGGAGACATGCTGGAGCGATGGAGCAACTGCATATTCAAGTCCACATTACACAGAGTTCTGGTGAATGGTCAAGATAGATATTCTATTGCATATTTTGTGGAACCTAGTCATGATTGTCTTGTGGAATGTTTGCCAACTTGCAAGTCAGAGAAAAACCCTCCCAAGTTTCCTCCGATTCTATGCAAGACATATTTAAGTCAGCGTTACCAAGAAACTCATGTTGATCTAAATACA

Protein sequence

MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT
Homology
BLAST of MC08g2519 vs. ExPASy Swiss-Prot
Match: C8VK14 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=hxnY PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.0e-40
Identity = 114/317 (35.96%), Postives = 167/317 (52.68%), Query Frame = 0

Query: 30  VSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKLFSLPLSNKMKLLR--NEKHRGYTP 89
           V  ++++C   GFF    H I  E    V   +K+ F+LPL  KM + +  N  +RGY  
Sbjct: 26  VQQVRESCEYNGFFQITGHRIPRELQVRVMDAAKRFFALPLEEKMAIDKNLNSFNRGYE- 85

Query: 90  TLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDP--DGERPFYGPNIWPA 149
                   SQ +      +L       KEG YIG  E+I  D P     R   GPN WPA
Sbjct: 86  -----LLRSQMLEVGTAPEL-------KEGLYIG--EEIGADHPYYINGRLNSGPNQWPA 145

Query: 150 EDLLPG---WREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGEPIAVLRLLH 209
              +P    +RE   +++    E+A+ +  ++AL LD+E  FFD     G  +A +R+LH
Sbjct: 146 --TVPDAQEFRETSMEYYHAVYELAKDVLAVLALTLDVEESFFDPLTEGG--VATMRMLH 205

Query: 210 YEAQ-ISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAFV 269
           Y +Q   E  K   G GAH+D+G ITLL  DE  GLQ+    DA    W  V P+ GA+V
Sbjct: 206 YPSQPKDEDEKLNRGIGAHTDFGCITLLLQDEVDGLQVL---DAPSGQWLDVQPVLGAYV 265

Query: 270 VNLGDMLERWSNCIFKSTLHRVL-VNGQDRYSIAYFVEPSHDCLVECLPTCKSEKNPPKF 329
           VNLGD++ R +N  +KS +HRV+  +G++RYSI +F   + D + +CLP C      PK+
Sbjct: 266 VNLGDLMMRMANDRYKSNIHRVINKSGRERYSIPFFFSGNPDHVCKCLPNCCKAGEQPKY 320

Query: 330 PPILCKTYLSQRYQETH 338
           PPI  +  +   Y++++
Sbjct: 326 PPITVEDMVRGAYKQSY 320

BLAST of MC08g2519 vs. ExPASy Swiss-Prot
Match: Q6K332 (2-oxoglutarate-dependent dioxygenase 33 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD33 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.8e-36
Identity = 102/298 (34.23%), Postives = 153/298 (51.34%), Query Frame = 0

Query: 21  LSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKLFSLPLSNKMKLLRNE 80
           ++  D+ Q V +L  AC + GFFY   HGIA+  M +V   ++K F LP   K+K+    
Sbjct: 26  VNDEDLLQVVQMLDDACREAGFFYVKGHGIADSLMKQVRDVTQKFFQLPYEEKLKIKMTP 85

Query: 81  K--HRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDPDGERPFY 140
           +  +RGY           Q V E+  +    +H       Y  IE   PG   D  +P  
Sbjct: 86  QSGYRGY-----------QRVGENITKGKPDMHEAI--DCYTPIE---PGKYGDLAKPMV 145

Query: 141 GPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGEPIAVL 200
           G N+WP       +  ++E + +   +++R I R IALAL   +D F+     G+P  V 
Sbjct: 146 GSNLWPKYP--SNFDVLLENYISLLRDLSRKIMRGIALALGAPVDAFE-GTTAGDPFWVC 205

Query: 201 RLLHY--EAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPL 260
           RL+ Y     I E  +   G GAH+DYGL+TL+  D+++     +++  +   W Y  P+
Sbjct: 206 RLIGYPVSTDIPEEQRTDTGCGAHTDYGLLTLVNQDDDICALEVRNQSGE---WIYAKPI 265

Query: 261 KGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRY--SIAYFVEPSHDCLVECLPTCK 313
            G FV N+GDML+ WSN I++ TLHRV VN   RY  S+A+F E + D  VE +  C+
Sbjct: 266 PGTFVCNIGDMLKVWSNGIYQPTLHRV-VNNSPRYRVSVAFFYESNFDAAVEPVEFCR 300

BLAST of MC08g2519 vs. ExPASy Swiss-Prot
Match: Q1ERI0 (2-oxoglutarate-dependent dioxygenase mpl2 OS=Monascus purpureus OX=5098 GN=mpl2 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-34
Identity = 101/290 (34.83%), Postives = 153/290 (52.76%), Query Frame = 0

Query: 20  DLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKLFSLPLSNKMKLLRN 79
           D +S    + +  ++ AC  TGFF  + HGI+      VFA + K F+LP   K +  RN
Sbjct: 22  DPNSDAARKVIDDVRAACTSTGFFQLLGHGISPALQQSVFAAAAKFFALPSDVKSR-CRN 81

Query: 80  EKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDP--DGERPF 139
              RGY P     +            +L +L  D KEG+  G  + IP DDP    +R F
Sbjct: 82  VGFRGYDPMASQSY------------ELGVL-PDLKEGFIAG--KDIPLDDPRVASQRFF 141

Query: 140 YGPNIWPAEDLLP--GWREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGEPI 199
            G N WP  +LLP   +R  +E+++   L++   +  ++A  L      FD+ +   +P 
Sbjct: 142 MGQNAWPPSELLPEANFRRPIEEYYQAMLKLCWVVLDLVAATLPYGPHVFDEFKE-NDPA 201

Query: 200 AVLRLLHY-EAQISEPSKG-IFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYV 259
             LRLLHY  A   + +KG   G+ AH+D+G ITLL  D++ GL++   +D +   W  V
Sbjct: 202 CPLRLLHYPPAPAPDVAKGRQLGSSAHTDFGAITLLLQDDHSGLEV---QDCETGEWIGV 261

Query: 260 APLKGAFVVNLGDMLERWSNCIFKSTLHRVL-VNGQDRYSIAYFVEPSHD 303
            P K A+VVNLGDM+ R +   +KS++HRV+  N  DRYS+ +F + + D
Sbjct: 262 PPNKDAYVVNLGDMMSRITRGHYKSSIHRVINQNLTDRYSVVFFFDGNLD 291

BLAST of MC08g2519 vs. ExPASy Swiss-Prot
Match: A0A159BP93 (2-oxoglutarate-dependent dioxygenase citB OS=Monascus ruber OX=89489 GN=citB PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-34
Identity = 101/290 (34.83%), Postives = 153/290 (52.76%), Query Frame = 0

Query: 20  DLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKLFSLPLSNKMKLLRN 79
           D +S    + +  ++ AC  TGFF  + HGI+      VFA + K F+LP   K +  RN
Sbjct: 22  DPNSDAARKVIDDVRAACTSTGFFQLLGHGISPALQQSVFAAAAKFFALPSDVKSR-CRN 81

Query: 80  EKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDP--DGERPF 139
              RGY P     +            +L +L  D KEG+  G  + IP DDP    +R F
Sbjct: 82  VGFRGYDPMASQSY------------ELGVL-PDLKEGFIAG--KDIPLDDPRVASQRFF 141

Query: 140 YGPNIWPAEDLLP--GWREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGEPI 199
            G N WP  +LLP   +R  +E+++   L++   +  ++A  L      FD+ +   +P 
Sbjct: 142 MGQNAWPPSELLPEANFRRPIEEYYQAMLKLCWVVLDLVAATLPYGPHVFDEFKE-NDPA 201

Query: 200 AVLRLLHY-EAQISEPSKG-IFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYV 259
             LRLLHY  A   + +KG   G+ AH+D+G ITLL  D++ GL++   +D +   W  V
Sbjct: 202 CPLRLLHYPPAPAPDVAKGRQLGSSAHTDFGAITLLLQDDHSGLEV---QDCETGEWIGV 261

Query: 260 APLKGAFVVNLGDMLERWSNCIFKSTLHRVL-VNGQDRYSIAYFVEPSHD 303
            P K A+VVNLGDM+ R +   +KS++HRV+  N  DRYS+ +F + + D
Sbjct: 262 PPNKDAYVVNLGDMMSRITRGHYKSSIHRVINQNLTDRYSVVFFFDGNLD 291

BLAST of MC08g2519 vs. ExPASy Swiss-Prot
Match: A0A5C1RDG7 (2-oxoglutarate-dependent dioxygenase OS=Didymella fabae OX=372025 GN=orf3 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.7e-33
Identity = 92/284 (32.39%), Postives = 149/284 (52.46%), Query Frame = 0

Query: 28  QSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKLFSLPLSNK--MKLLRNEKHRGY 87
           Q V  +++AC  TGFF    HG++++ ++++FA S K F+LP   K  +   +N   RGY
Sbjct: 30  QVVDQVRKACTSTGFFQLTGHGLSQDVVEDIFAASAKFFALPKDVKQGLNFTKNLGFRGY 89

Query: 88  TPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDPDGERPFYGPNIWPA 147
                     +Q VYE        +  D KEG+  GI+          +R F G N+WP 
Sbjct: 90  E------LIGAQ-VYE------SDVLPDLKEGFLAGIDLPFEDMRVQNKRFFAGQNVWPP 149

Query: 148 EDLLP--GWREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGEPIAVLRLLHY 207
            D+LP   +RE +EK++   +++   +  ++A  L    D FD+ +   EP   LRLLHY
Sbjct: 150 PDVLPYAEFREPVEKYYKSIMQLCFTVMDLVAATLPYGPDVFDEWKS-HEPACPLRLLHY 209

Query: 208 ---EAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYVAPLKGAF 267
               A ++  ++ + G+ AH+D+G +TLL  D + GL++          W  V P  GAF
Sbjct: 210 PPTPAHVAGKTRQL-GSSAHTDFGALTLLLQDSHEGLEVLNHDTGD---WVLVPPKPGAF 269

Query: 268 VVNLGDMLERWSNCIFKSTLHRVLVNG--QDRYSIAYFVEPSHD 303
           VVN+ DM+   +   +KS+ HRV+     +DRYS+ +F++ + D
Sbjct: 270 VVNIADMMTMVTVGEYKSSKHRVINRNETEDRYSVVFFMDGNVD 295

BLAST of MC08g2519 vs. NCBI nr
Match: XP_022154755.1 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 [Momordica charantia])

HSP 1 Score: 702 bits (1812), Expect = 2.96e-255
Identity = 341/343 (99.42%), Postives = 341/343 (99.42%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA
Sbjct: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLH-RDYKEGYY 120
           QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLH  DYKEGYY
Sbjct: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHIGDYKEGYY 120

Query: 121 IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD 180
           IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD
Sbjct: 121 IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD 180

Query: 181 LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI 240
           LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI
Sbjct: 181 LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI 240

Query: 241 CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP 300
           CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP
Sbjct: 241 CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP 300

Query: 301 SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT
Sbjct: 301 SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 343

BLAST of MC08g2519 vs. NCBI nr
Match: XP_038877416.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X2 [Benincasa hispida])

HSP 1 Score: 586 bits (1510), Expect = 4.87e-209
Identity = 284/342 (83.04%), Postives = 306/342 (89.47%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME   E+D  PKFLALN IDLSSSD+++SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 87

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPLS+KMKLLRNEKHRGYTP L           +   +    +  DYKEGYYI
Sbjct: 88  QSKRLFSLPLSDKMKLLRNEKHRGYTPIL-----------DELLDPDNQIRGDYKEGYYI 147

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEK PGDD DGERPFYGPNIWPAEDLLPGWR+IMEKFHTQALEVARAI+R+IALALDL
Sbjct: 148 GIEEKNPGDDSDGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDL 207

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK E LGEPIA+LRLLHYE Q+SEP+KGIFGAGAH+DYGLITLLATDENLGLQIC
Sbjct: 208 ENDFFDKAETLGEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQIC 267

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDKDAKPQIW+YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP+
Sbjct: 268 KDKDAKPQIWQYVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPN 327

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           HDCLVECLPTCKS+KNPPKFPPI C+TYLSQRY+ETHVDL+T
Sbjct: 328 HDCLVECLPTCKSDKNPPKFPPIRCRTYLSQRYKETHVDLST 358

BLAST of MC08g2519 vs. NCBI nr
Match: XP_038877418.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X4 [Benincasa hispida])

HSP 1 Score: 585 bits (1507), Expect = 5.35e-209
Identity = 284/342 (83.04%), Postives = 306/342 (89.47%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME   E+D  PKFLALN IDLSSSD+++SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 1   MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPLS+KMKLLRNEKHRGYTP L  +               ++   DYKEGYYI
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDN----------QIRVGDYKEGYYI 120

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEK PGDD DGERPFYGPNIWPAEDLLPGWR+IMEKFHTQALEVARAI+R+IALALDL
Sbjct: 121 GIEEKNPGDDSDGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDL 180

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK E LGEPIA+LRLLHYE Q+SEP+KGIFGAGAH+DYGLITLLATDENLGLQIC
Sbjct: 181 ENDFFDKAETLGEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQIC 240

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDKDAKPQIW+YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP+
Sbjct: 241 KDKDAKPQIWQYVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPN 300

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           HDCLVECLPTCKS+KNPPKFPPI C+TYLSQRY+ETHVDL+T
Sbjct: 301 HDCLVECLPTCKSDKNPPKFPPIRCRTYLSQRYKETHVDLST 332

BLAST of MC08g2519 vs. NCBI nr
Match: XP_038877415.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X1 [Benincasa hispida])

HSP 1 Score: 585 bits (1507), Expect = 1.45e-208
Identity = 284/342 (83.04%), Postives = 306/342 (89.47%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME   E+D  PKFLALN IDLSSSD+++SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 87

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPLS+KMKLLRNEKHRGYTP L  +               ++   DYKEGYYI
Sbjct: 88  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDN----------QIRVGDYKEGYYI 147

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEK PGDD DGERPFYGPNIWPAEDLLPGWR+IMEKFHTQALEVARAI+R+IALALDL
Sbjct: 148 GIEEKNPGDDSDGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDL 207

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK E LGEPIA+LRLLHYE Q+SEP+KGIFGAGAH+DYGLITLLATDENLGLQIC
Sbjct: 208 ENDFFDKAETLGEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQIC 267

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDKDAKPQIW+YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP+
Sbjct: 268 KDKDAKPQIWQYVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPN 327

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           HDCLVECLPTCKS+KNPPKFPPI C+TYLSQRY+ETHVDL+T
Sbjct: 328 HDCLVECLPTCKSDKNPPKFPPIRCRTYLSQRYKETHVDLST 359

BLAST of MC08g2519 vs. NCBI nr
Match: XP_023545999.1 (2-oxoglutarate-dependent dioxygenase mpl2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578 bits (1490), Expect = 3.36e-206
Identity = 283/342 (82.75%), Postives = 302/342 (88.30%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME R  +DP P F ALN IDLSSSDIH SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPLS+KMKLLRNEKHRGYTP L           +   +    +  DYKEGYYI
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPIL-----------DELLDPDNQIRGDYKEGYYI 120

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEKIPGDD  GE PFYGPNIWPAEDLLPGWR+IMEKFH +ALEVARAI+R+IALAL L
Sbjct: 121 GIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGL 180

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK EMLGEPIAVLRLLHYE QISEP+KGIFGAGAH+DYGLITLLATDEN GLQIC
Sbjct: 181 ENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQIC 240

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDK+AKPQIWEYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYSIAYFVEPS
Sbjct: 241 KDKEAKPQIWEYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPS 300

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           HDCLVECLPTCKS++NPPKFPPILCKTYLSQRY+ETHVDL+T
Sbjct: 301 HDCLVECLPTCKSDENPPKFPPILCKTYLSQRYKETHVDLDT 331

BLAST of MC08g2519 vs. ExPASy TrEMBL
Match: A0A6J1DKI9 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021931 PE=3 SV=1)

HSP 1 Score: 702 bits (1812), Expect = 1.44e-255
Identity = 341/343 (99.42%), Postives = 341/343 (99.42%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA
Sbjct: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLH-RDYKEGYY 120
           QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLH  DYKEGYY
Sbjct: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHIGDYKEGYY 120

Query: 121 IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD 180
           IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD
Sbjct: 121 IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD 180

Query: 181 LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI 240
           LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI
Sbjct: 181 LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI 240

Query: 241 CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP 300
           CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP
Sbjct: 241 CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP 300

Query: 301 SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT
Sbjct: 301 SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 343

BLAST of MC08g2519 vs. ExPASy TrEMBL
Match: A0A6J1G3R7 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 577 bits (1487), Expect = 4.66e-206
Identity = 281/342 (82.16%), Postives = 302/342 (88.30%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME R  +DP+P F ALN IDLSSSDIH SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPLS+KMKLLRNEKHRGYTP L           +   +    +  DYKEGYYI
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPIL-----------DELLDPDNQIRGDYKEGYYI 120

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEKIPGDD  GE PFYGPNIWPAEDLLPGWR+IMEKFH +ALEVARAI+R+IALAL L
Sbjct: 121 GIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGL 180

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK EMLGEPIAVLRLLHYE QISEP+KGIFGAGAH+DYGLITLLATDEN GLQIC
Sbjct: 181 ENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQIC 240

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDK+AKPQ+WEYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYSIAYFVEP 
Sbjct: 241 KDKEAKPQVWEYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPR 300

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           HDCLVECLPTCKS++NPPKFPPILCKTYLSQRY+ETHVDL+T
Sbjct: 301 HDCLVECLPTCKSDENPPKFPPILCKTYLSQRYKETHVDLDT 331

BLAST of MC08g2519 vs. ExPASy TrEMBL
Match: A0A6J1G3P3 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 1.38e-205
Identity = 281/342 (82.16%), Postives = 302/342 (88.30%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME R  +DP+P F ALN IDLSSSDIH SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPLS+KMKLLRNEKHRGYTP L  +               ++   DYKEGYYI
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDN----------QIRVGDYKEGYYI 120

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEKIPGDD  GE PFYGPNIWPAEDLLPGWR+IMEKFH +ALEVARAI+R+IALAL L
Sbjct: 121 GIEEKIPGDDSGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGL 180

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK EMLGEPIAVLRLLHYE QISEP+KGIFGAGAH+DYGLITLLATDEN GLQIC
Sbjct: 181 ENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQIC 240

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDK+AKPQ+WEYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYSIAYFVEP 
Sbjct: 241 KDKEAKPQVWEYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPR 300

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           HDCLVECLPTCKS++NPPKFPPILCKTYLSQRY+ETHVDL+T
Sbjct: 301 HDCLVECLPTCKSDENPPKFPPILCKTYLSQRYKETHVDLDT 332

BLAST of MC08g2519 vs. ExPASy TrEMBL
Match: A0A6J1KDA3 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 8.97e-204
Identity = 280/342 (81.87%), Postives = 300/342 (87.72%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME R  +DP P F ALN IDLSSSDIH SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPL +KMKLLRNEKHRGYTP L           +   +    +  DYKEGYYI
Sbjct: 61  QSKRLFSLPLCDKMKLLRNEKHRGYTPIL-----------DELLDPENQIRGDYKEGYYI 120

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEK PGDD  GE PFYGPNIWPAEDLLPGWR+IMEKFH +ALEVARAI+R+IALAL L
Sbjct: 121 GIEEKNPGDDSGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGL 180

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK EMLGEPIAVLRLLHYE QISEP+KGIFGAGAH+DYGLITLLATDEN GLQIC
Sbjct: 181 ENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQIC 240

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDKDAKP+IWEYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYSIAYFVEPS
Sbjct: 241 KDKDAKPRIWEYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPS 300

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           +DCLVECLPTCKS++NPPKFPPILCKTYLSQRY+ETHVDL+T
Sbjct: 301 NDCLVECLPTCKSDENPPKFPPILCKTYLSQRYKETHVDLDT 331

BLAST of MC08g2519 vs. ExPASy TrEMBL
Match: A0A6J1KFK8 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 2.66e-203
Identity = 281/342 (82.16%), Postives = 301/342 (88.01%), Query Frame = 0

Query: 1   MEIRAEDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFA 60
           ME R  +DP P F ALN IDLSSSDIH SVSLLKQACLDTGFFYAVNHGI EEFMDEVFA
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSK+LFSLPL +KMKLLRNEKHRGYTP L           E    + ++   DYKEGYYI
Sbjct: 61  QSKRLFSLPLCDKMKLLRNEKHRGYTPILD----------ELLDPENQIRVGDYKEGYYI 120

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIEEK PGDD  GE PFYGPNIWPAEDLLPGWR+IMEKFH +ALEVARAI+R+IALAL L
Sbjct: 121 GIEEKNPGDDSGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGL 180

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           E DFFDK EMLGEPIAVLRLLHYE QISEP+KGIFGAGAH+DYGLITLLATDEN GLQIC
Sbjct: 181 ENDFFDKSEMLGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQIC 240

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDKDAKP+IWEYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQDRYSIAYFVEPS
Sbjct: 241 KDKDAKPRIWEYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPS 300

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLNT 342
           +DCLVECLPTCKS++NPPKFPPILCKTYLSQRY+ETHVDL+T
Sbjct: 301 NDCLVECLPTCKSDENPPKFPPILCKTYLSQRYKETHVDLDT 332

BLAST of MC08g2519 vs. TAIR 10
Match: AT1G35190.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 3.9e-129
Identity = 215/336 (63.99%), Postives = 265/336 (78.87%), Query Frame = 0

Query: 6   EDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKL 65
           E+    K  +LN IDL++ D++ SV  LKQACLD GFFY +NHGI+EEFMD+VF QSKKL
Sbjct: 2   ENHTTMKVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKL 61

Query: 66  FSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEK 125
           F+LPL  KMK+LRNEKHRGYTP L           +   +    ++ D+KEGYYIGIE  
Sbjct: 62  FALPLEEKMKVLRNEKHRGYTPVL-----------DELLDPKNQINGDHKEGYYIGIE-- 121

Query: 126 IPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDLEIDFF 185
           +P DDP  ++PFYGPN WP  D+LPGWRE MEK+H +AL V+ AI+R++ALALDL++ +F
Sbjct: 122 VPKDDPHWDKPFYGPNPWPDADVLPGWRETMEKYHQEALRVSMAIARLLALALDLDVGYF 181

Query: 186 DKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDA 245
           D+ EMLG+PIA +RLL Y+  IS+PSKGI+  GAHSD+G++TLLATD  +GLQICKDK+A
Sbjct: 182 DRTEMLGKPIATMRLLRYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNA 241

Query: 246 KPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLV 305
            PQ WEYV P+KGAF+VNLGDMLERWSN  FKSTLHRVL NGQ+RYSI +FVEP+HDCLV
Sbjct: 242 MPQKWEYVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLV 301

Query: 306 ECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLN 342
           ECLPTCKSE   PK+PPI C TYL+QRY+ETH +L+
Sbjct: 302 ECLPTCKSESELPKYPPIKCSTYLTQRYEETHANLS 323

BLAST of MC08g2519 vs. TAIR 10
Match: AT1G35190.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 419.1 bits (1076), Expect = 3.5e-117
Identity = 203/336 (60.42%), Postives = 245/336 (72.92%), Query Frame = 0

Query: 6   EDDPAPKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVFAQSKKL 65
           E+    K  +LN IDL++ D++ SV  LKQACLD GFFY +NHGI+EEFMD+VF QSKKL
Sbjct: 2   ENHTTMKVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKL 61

Query: 66  FSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEK 125
           F+LPL  KMK+LRNEKHRGYTP L           +   +    ++ D+KEGYYIGIE  
Sbjct: 62  FALPLEEKMKVLRNEKHRGYTPVL-----------DELLDPKNQINGDHKEGYYIGIE-- 121

Query: 126 IPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDLEIDFF 185
           +P DDP  ++PFYGPN WP  D+LPGWRE MEK+H +AL                     
Sbjct: 122 VPKDDPHWDKPFYGPNPWPDADVLPGWRETMEKYHQEAL--------------------- 181

Query: 186 DKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDA 245
              EMLG+PIA +RLL Y+  IS+PSKGI+  GAHSD+G++TLLATD  +GLQICKDK+A
Sbjct: 182 --TEMLGKPIATMRLLRYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNA 241

Query: 246 KPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLV 305
            PQ WEYV P+KGAF+VNLGDMLERWSN  FKSTLHRVL NGQ+RYSI +FVEP+HDCLV
Sbjct: 242 MPQKWEYVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLV 300

Query: 306 ECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLN 342
           ECLPTCKSE   PK+PPI C TYL+QRY+ETH +L+
Sbjct: 302 ECLPTCKSESELPKYPPIKCSTYLTQRYEETHANLS 300

BLAST of MC08g2519 vs. TAIR 10
Match: AT3G46490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 415.6 bits (1067), Expect = 3.8e-116
Identity = 196/341 (57.48%), Postives = 252/341 (73.90%), Query Frame = 0

Query: 1   MEIRAEDDPA-PKFLALNRIDLSSSDIHQSVSLLKQACLDTGFFYAVNHGIAEEFMDEVF 60
           ME + E++ +  K  +L  IDL +SD+HQS  LLKQACLD+GFFY +NHGI+EE  DE F
Sbjct: 1   MENKTEEEVSIIKVSSLTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAF 60

Query: 61  AQSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYY 120
             SKK F+LPL  KMK+LRNEK+RGY P            ++   +    +  DYKEG+ 
Sbjct: 61  EHSKKFFALPLEEKMKVLRNEKYRGYAP-----------FHDSLLDPENQVRGDYKEGFT 120

Query: 121 IGIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALD 180
           IG E     D P  ++PF+ PNIWP  D+LPGWRE MEK++ +AL V ++I++I+ALALD
Sbjct: 121 IGFEGS--KDGPHWDKPFHSPNIWPNPDVLPGWRETMEKYYQEALRVCKSIAKIMALALD 180

Query: 181 LEIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQI 240
           L++D+F+ PEMLG PIA + L HYE + S+PSKGI+  GAHSD+G+++LLATD  +GLQI
Sbjct: 181 LDVDYFNTPEMLGNPIADMVLFHYEGK-SDPSKGIYACGAHSDFGMMSLLATDGVMGLQI 240

Query: 241 CKDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEP 300
           CKDKD KPQ WEY   +KGA++VNLGD+LERWSN  FKSTLHRVL NGQDRYSI +F++P
Sbjct: 241 CKDKDVKPQKWEYTPSIKGAYIVNLGDLLERWSNGYFKSTLHRVLGNGQDRYSIPFFLKP 300

Query: 301 SHDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDL 341
           SHDC++ECLPTC+SE N PK+P I C TY+SQRY+  H  L
Sbjct: 301 SHDCIIECLPTCQSENNLPKYPAIKCSTYISQRYKAVHAHL 327

BLAST of MC08g2519 vs. TAIR 10
Match: AT3G46480.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 2.8e-95
Identity = 167/281 (59.43%), Postives = 205/281 (72.95%), Query Frame = 0

Query: 61  QSKKLFSLPLSNKMKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYI 120
           QSKK F+LPL  KMK+LRNEKHRGY+P L  +            +    +  DYKE ++I
Sbjct: 36  QSKKFFALPLEEKMKVLRNEKHRGYSPVLDQIL-----------DPENQVDGDYKESFFI 95

Query: 121 GIEEKIPGDDPDGERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDL 180
           GIE                        +LPGWR  MEK+H +AL V +AI+R++ALALDL
Sbjct: 96  GIEV-----------------------VLPGWRATMEKYHQEALRVCKAIARLLALALDL 155

Query: 181 EIDFFDKPEMLGEPIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQIC 240
           + ++FDKPEMLG PIAV+RLL YE  +S+P KGIFG GAHSDYG++TLLATD   GLQIC
Sbjct: 156 DTNYFDKPEMLGNPIAVMRLLRYEG-MSDPLKGIFGCGAHSDYGMLTLLATDSVTGLQIC 215

Query: 241 KDKDAKPQIWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPS 300
           KDKD KP+ WEYV  +KGA++VNLGD+LERWSN IFKSTLHRVL NGQDRYSI +F+EPS
Sbjct: 216 KDKDVKPRKWEYVPSIKGAYIVNLGDLLERWSNGIFKSTLHRVLGNGQDRYSIPFFIEPS 275

Query: 301 HDCLVECLPTCKSEKNPPKFPPILCKTYLSQRYQETHVDLN 342
           HDCLVECLPTC+SE N PK+P I C T+L+QRYQ++HV+L+
Sbjct: 276 HDCLVECLPTCQSENNLPKYPAIKCSTFLTQRYQQSHVNLS 281

BLAST of MC08g2519 vs. TAIR 10
Match: AT3G46500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 9.2e-94
Identity = 154/268 (57.46%), Postives = 202/268 (75.37%), Query Frame = 0

Query: 74  MKLLRNEKHRGYTPTLKYVFCNSQFVYEHCKEKLKLLHRDYKEGYYIGIEEKIPGDDPDG 133
           MK+L+NEKH+GY+P L  +  N              +H DYKE ++IGIE          
Sbjct: 1   MKVLKNEKHQGYSPVLSQISDNQ-------------IHGDYKESFFIGIE-------GSN 60

Query: 134 ERPFYGPNIWPAEDLLPGWREIMEKFHTQALEVARAISRIIALALDLEIDFFDKPEMLGE 193
           + PF   NIWP  D+L GW+  MEK+H +AL V +AI+R++ALAL+++ D+FD PEMLG 
Sbjct: 61  DTPFCRANIWPNPDVLSGWQATMEKYHQEALRVCKAIARVLALALNVDGDYFDTPEMLGN 120

Query: 194 PIAVLRLLHYEAQISEPSKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQIWEYV 253
           P+  +RLLHYE  +S+PSKGI+G G HSD+G++TLL TD  +GLQICKD+D KP+ WEY+
Sbjct: 121 PLTFMRLLHYEG-MSDPSKGIYGCGPHSDFGMMTLLGTDSVMGLQICKDRDVKPRKWEYI 180

Query: 254 APLKGAFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPSHDCLVECLPTCKS 313
             +KGA++VN+GD+LERWSN IFKSTLHRVL NGQDRYSIA+F++PSHDC+VECLPTC+S
Sbjct: 181 LSIKGAYIVNIGDLLERWSNGIFKSTLHRVLGNGQDRYSIAFFLQPSHDCIVECLPTCQS 240

Query: 314 EKNPPKFPPILCKTYLSQRYQETHVDLN 342
           E NPPK+P I C TYL+QRYQ++ VDL+
Sbjct: 241 ENNPPKYPAIKCSTYLTQRYQDSQVDLS 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C8VK142.0e-4035.962-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FG... [more]
Q6K3323.8e-3634.232-oxoglutarate-dependent dioxygenase 33 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q1ERI01.6e-3434.832-oxoglutarate-dependent dioxygenase mpl2 OS=Monascus purpureus OX=5098 GN=mpl2 ... [more]
A0A159BP931.6e-3434.832-oxoglutarate-dependent dioxygenase citB OS=Monascus ruber OX=89489 GN=citB PE=... [more]
A0A5C1RDG76.7e-3332.392-oxoglutarate-dependent dioxygenase OS=Didymella fabae OX=372025 GN=orf3 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022154755.12.96e-25599.422-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 [Momordica charantia][more]
XP_038877416.14.87e-20983.042-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X2 [Benincasa hispid... [more]
XP_038877418.15.35e-20983.042-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X4 [Benincasa hispid... [more]
XP_038877415.11.45e-20883.042-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X1 [Benincasa hispid... [more]
XP_023545999.13.36e-20682.752-oxoglutarate-dependent dioxygenase mpl2 isoform X2 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
A0A6J1DKI91.44e-25599.422-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Momordica charantia... [more]
A0A6J1G3R74.66e-20682.162-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1G3P31.38e-20582.162-oxoglutarate-dependent dioxygenase mpl2-like isoform X1 OS=Cucurbita moschata ... [more]
A0A6J1KDA38.97e-20481.872-oxoglutarate-Fe(II) type oxidoreductase-like isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1KFK82.66e-20382.162-oxoglutarate-Fe(II) type oxidoreductase-like isoform X1 OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
AT1G35190.13.9e-12963.992-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G35190.23.5e-11760.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46490.13.8e-11657.482-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46480.12.8e-9559.432-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46500.19.2e-9457.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 33..50
score: 59.6
coord: 260..286
score: 29.97
coord: 287..305
score: 23.44
NoneNo IPR availablePANTHERPTHR10209:SF7682OG-FE(II) OXYGENASE FAMILY OXIDOREDUCTASEcoord: 15..333
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 15..333
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 16..337
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 19..146
e-value: 1.1E-26
score: 93.9
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 8..340
e-value: 1.3E-93
score: 316.1
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 196..299
e-value: 1.7E-22
score: 79.8
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 194..299
score: 11.574911

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2519.1MC08g2519.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding