MC08g2423 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2423
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	calmodulin-like protein 7
Location	MC08: 33075155 .. 33075742 (+)
RNA-Seq Expression	MC08g2423
Synteny	MC08g2423

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATCCATGATGAACATTCTGTTATTGGCAGTTTTGTTCGTTGCAGGCCTCGTCAATTTCCTCGTGTATTTCCCCACCAAGCGATTCTCCGCCTGGCTCAAACAGAGCCCTACAAAGCCCGCCGCCGTCGCCGCCGCCGTCGCCCCCCCACAGCCGCCGACGGAGCTGAGGAAGATATTCCGGACATTTGACAAGAACAACGACGGCTACATAACCAGAAAGGAGCTGACGGAATCGCTGAGAAACTGGATCGCAGAGAGGGAGGCAGAGGAATTGTTCGAAGGGGTGGACGAAAATGGAGATGGGCTGATCGATTTCGAGGAGTTCTGTGTTCTGGCGGAGAAATTGAGGAAGGAGAAAGAGGAGGAAGAAGAAGATGATGATGAGGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGGGTGATTTCTGTGGAGGAATTGGGATTGGTTCTGTCATGTTTGGGGCTCAAGGAAGGGAAAATGGCTGAGAATTGCAGGGAGATGATCAGGAAAGTGGATTTGGATGGAGATGGAATGGTCAATTTTGAGGAGTTTAAGAGGATGATGAGAAATGGG

mRNA sequence

CAATCCATGATGAACATTCTGTTATTGGCAGTTTTGTTCGTTGCAGGCCTCGTCAATTTCCTCGTGTATTTCCCCACCAAGCGATTCTCCGCCTGGCTCAAACAGAGCCCTACAAAGCCCGCCGCCGTCGCCGCCGCCGTCGCCCCCCCACAGCCGCCGACGGAGCTGAGGAAGATATTCCGGACATTTGACAAGAACAACGACGGCTACATAACCAGAAAGGAGCTGACGGAATCGCTGAGAAACTGGATCGCAGAGAGGGAGGCAGAGGAATTGTTCGAAGGGGTGGACGAAAATGGAGATGGGCTGATCGATTTCGAGGAGTTCTGTGTTCTGGCGGAGAAATTGAGGAAGGAGAAAGAGGAGGAAGAAGAAGATGATGATGAGGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGGGTGATTTCTGTGGAGGAATTGGGATTGGTTCTGTCATGTTTGGGGCTCAAGGAAGGGAAAATGGCTGAGAATTGCAGGGAGATGATCAGGAAAGTGGATTTGGATGGAGATGGAATGGTCAATTTTGAGGAGTTTAAGAGGATGATGAGAAATGGG

Coding sequence (CDS)

CAATCCATGATGAACATTCTGTTATTGGCAGTTTTGTTCGTTGCAGGCCTCGTCAATTTCCTCGTGTATTTCCCCACCAAGCGATTCTCCGCCTGGCTCAAACAGAGCCCTACAAAGCCCGCCGCCGTCGCCGCCGCCGTCGCCCCCCCACAGCCGCCGACGGAGCTGAGGAAGATATTCCGGACATTTGACAAGAACAACGACGGCTACATAACCAGAAAGGAGCTGACGGAATCGCTGAGAAACTGGATCGCAGAGAGGGAGGCAGAGGAATTGTTCGAAGGGGTGGACGAAAATGGAGATGGGCTGATCGATTTCGAGGAGTTCTGTGTTCTGGCGGAGAAATTGAGGAAGGAGAAAGAGGAGGAAGAAGAAGATGATGATGAGGAACTGAGAGAGGCTTTTGGAGTGTTTGATAAGGACAAAGATGGGGTGATTTCTGTGGAGGAATTGGGATTGGTTCTGTCATGTTTGGGGCTCAAGGAAGGGAAAATGGCTGAGAATTGCAGGGAGATGATCAGGAAAGTGGATTTGGATGGAGATGGAATGGTCAATTTTGAGGAGTTTAAGAGGATGATGAGAAATGGG

Protein sequence

QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIFRTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG

Homology

[Close]

BLAST of MC08g2423 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.4e-35
Identity = 75/147 (51.02%), Postives = 112/147 (76.19%), Query Frame = 0


Query: 53  PTELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEF 112
           PTEL+++F+ FDKN DG IT KEL+E+LR+   +I ++E  ++ E +D NGDG +D +EF
Sbjct: 3   PTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF 62

Query: 113 CVLAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENC 172
             L + +  E++EEEED    ++EAF VFD++ DG I+V+EL  VLS LGLK+GK  ++C
Sbjct: 63  GELYKTIMDEEDEEEED----MKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDC 122

Query: 173 REMIRKVDLDGDGMVNFEEFKRMMRNG 197
           ++MI+KVD+DGDG VN++EF++MM+ G
Sbjct: 123 KKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of MC08g2423 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 7.8e-34
Identity = 74/145 (51.03%), Postives = 107/145 (73.79%), Query Frame = 0


Query: 55  ELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEFCV 114
           EL +IF+ FD+N DG IT++EL +SL N   +I +++  ++ E +D NGDG +D EEF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 115 LAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCRE 174
           L + +      EE D++E++REAF VFD+++DG I+VEEL  VL+ LGLK+G+  E+C+ 
Sbjct: 65  LYQTIM-----EERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKR 124

Query: 175 MIRKVDLDGDGMVNFEEFKRMMRNG 197
           MI KVD+DGDGMVNF+EFK+MM+ G
Sbjct: 125 MISKVDVDGDGMVNFKEFKQMMKGG 144

[Close]

BLAST of MC08g2423 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 5.6e-32
Identity = 76/183 (41.53%), Postives = 120/183 (65.57%), Query Frame = 0


Query: 24  FPTKRFSAWL-KQSPTKPAAV----AAAVAPPQ--PPTELRKIFRTFDKNNDGYITRKEL 83
           FP   F   L K SP  P+ +    +++ AP +   P+EL+++F+ FDKN DG IT++EL
Sbjct: 27  FPLSWFDKTLHKNSPPSPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEEL 86

Query: 84  TESLRN---WIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEKEEEEEDDDEELRE 143
            +SL N   +I +++  ++   +D NGDG +D +EF  L   +  E   + E ++E++++
Sbjct: 87  NDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMKD 146

Query: 144 AFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDGDGMVNFEEFKRMM 197
           AF VFD+D DG I+VEEL  V++ LGLK+GK  + C++MI +VD DGDG VN++EF +MM
Sbjct: 147 AFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

[Close]

BLAST of MC08g2423 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-31
Identity = 73/146 (50.00%), Postives = 103/146 (70.55%), Query Frame = 0


Query: 54  TELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEFC 113
           TEL ++F+ FDK+ DG IT KEL ES +N    I E E  ++ + +D NGDG +D EEF 
Sbjct: 4   TELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFG 63

Query: 114 VLAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCR 173
            L + +    E+E+E  +E+++EAF VFD++ DG I+V+EL  VLS LGLK+GK  E CR
Sbjct: 64  ELYKTIM--VEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 123

Query: 174 EMIRKVDLDGDGMVNFEEFKRMMRNG 197
           +MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 124 KMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of MC08g2423 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.0e-30
Identity = 71/145 (48.97%), Postives = 102/145 (70.34%), Query Frame = 0


Query: 55  ELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEFCV 114
           EL ++F+ FDKN DG I + EL +  ++    + E E  E+   +D NGDG +D +EF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 115 LAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCRE 174
           L +++ +EKEEE     E++REAF VFD++ DG I+ EEL  VL+ +GLK+G+  E+C++
Sbjct: 65  LYQEMVEEKEEE-----EDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKK 124

Query: 175 MIRKVDLDGDGMVNFEEFKRMMRNG 197
           MI KVD+DGDGMVNF+EFK+MMR G
Sbjct: 125 MISKVDVDGDGMVNFKEFKQMMRGG 144

[Close]

BLAST of MC08g2423 vs. NCBI nr
Match: XP_022143005.1 (calmodulin-like protein 7 [Momordica charantia])

HSP 1 Score: 379 bits (974), Expect = 2.42e-131
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIF 60
           QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIF
Sbjct: 67  QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIF 126

Query: 61  RTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEK 120
           RTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEK
Sbjct: 127 RTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEK 186

Query: 121 EEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDG 180
           EEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDG
Sbjct: 187 EEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDG 246

Query: 181 DGMVNFEEFKRMMRNG 196
           DGMVNFEEFKRMMRNG
Sbjct: 247 DGMVNFEEFKRMMRNG 262

[Close]

BLAST of MC08g2423 vs. NCBI nr
Match: XP_023545557.1 (calmodulin-like protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265 bits (677), Expect = 6.51e-87
Identity = 144/213 (67.61%), Postives = 174/213 (81.69%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAW---LKQSPT----KPAAVAA-AVAPPQP 60
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW   +K SP     KP AV   + AP + 
Sbjct: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKK 61

Query: 61  PTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 120
            +E++K+F TFDKNNDG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121

Query: 121 CVLAEKL------RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEG 180
           CVL EKL      ++ + E+E++D++ELREAFGVFDKDKDGVISVEELGLVLSCLG+ EG
Sbjct: 122 CVLGEKLVMGFEVKQRRVEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMNEG 181

Query: 181 KMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
           K  ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G
Sbjct: 182 KKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 214

[Close]

BLAST of MC08g2423 vs. NCBI nr
Match: XP_022999398.1 (calmodulin-like protein 7 [Cucurbita maxima])

HSP 1 Score: 264 bits (674), Expect = 1.74e-86
Identity = 144/212 (67.92%), Postives = 169/212 (79.72%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAV---AAAVAPPQPP---- 60
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW +     P +V     AV PP P     
Sbjct: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEKK 61

Query: 61  -TELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 120
            +E++K+F TFDKNNDG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121

Query: 121 CVLAEKL-----RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGK 180
           CVL EKL      K++  E+ED+DE LREAFGVFDKDKDGVISVEELGLVLSCLG+ EGK
Sbjct: 122 CVLGEKLVVGFEEKQRRIEDEDEDE-LREAFGVFDKDKDGVISVEELGLVLSCLGMNEGK 181

Query: 181 MAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
             ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G
Sbjct: 182 KLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 212

[Close]

BLAST of MC08g2423 vs. NCBI nr
Match: XP_022946838.1 (calmodulin-like protein 7 [Cucurbita moschata])

HSP 1 Score: 264 bits (674), Expect = 1.99e-86
Identity = 144/215 (66.98%), Postives = 174/215 (80.93%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAW---LKQSPT----KPAAVAA-AVAPPQP 60
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW   +K SP     KP AV   + AP + 
Sbjct: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKK 61

Query: 61  PTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 120
            +E++K+F TFDKN DG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121

Query: 121 CVLAEKL--------RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLK 180
           CVL EKL        R+ ++E+E++D++ELREAFGVFDKDKDGVISVEELGLVLSCLG+ 
Sbjct: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMN 181

Query: 181 EGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
           EGK  ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G
Sbjct: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 216

[Close]

BLAST of MC08g2423 vs. NCBI nr
Match: KAG6599380.1 (Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263 bits (671), Expect = 5.69e-86
Identity = 143/215 (66.51%), Postives = 174/215 (80.93%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAW---LKQSPT----KPAAVAA-AVAPPQP 60
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW   +K SP     KP AV   + AP + 
Sbjct: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKK 61

Query: 61  PTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 120
            +E++K+F TFDKN DG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121

Query: 121 CVLAEKL--------RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLK 180
           CVL EKL        R+ ++E+E++D++ELREAFGVFDKDKDG+ISVEELGLVLSCLG+ 
Sbjct: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGMISVEELGLVLSCLGMN 181

Query: 181 EGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
           EGK  ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G
Sbjct: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 216

[Close]

BLAST of MC08g2423 vs. ExPASy TrEMBL
Match: A0A6J1CMI5 (calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV=1)

HSP 1 Score: 379 bits (974), Expect = 1.17e-131
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIF 60
           QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIF
Sbjct: 67  QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAVAAAVAPPQPPTELRKIF 126

Query: 61  RTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEK 120
           RTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEK
Sbjct: 127 RTFDKNNDGYITRKELTESLRNWIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEK 186

Query: 121 EEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDG 180
           EEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDG
Sbjct: 187 EEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDG 246

Query: 181 DGMVNFEEFKRMMRNG 196
           DGMVNFEEFKRMMRNG
Sbjct: 247 DGMVNFEEFKRMMRNG 262

[Close]

BLAST of MC08g2423 vs. ExPASy TrEMBL
Match: A0A6J1KJL5 (calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1)

HSP 1 Score: 264 bits (674), Expect = 8.43e-87
Identity = 144/212 (67.92%), Postives = 169/212 (79.72%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQSPTKPAAV---AAAVAPPQPP---- 60
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW +     P +V     AV PP P     
Sbjct: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEKK 61

Query: 61  -TELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 120
            +E++K+F TFDKNNDG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 62  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121

Query: 121 CVLAEKL-----RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGK 180
           CVL EKL      K++  E+ED+DE LREAFGVFDKDKDGVISVEELGLVLSCLG+ EGK
Sbjct: 122 CVLGEKLVVGFEEKQRRIEDEDEDE-LREAFGVFDKDKDGVISVEELGLVLSCLGMNEGK 181

Query: 181 MAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
             ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G
Sbjct: 182 KLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 212

[Close]

BLAST of MC08g2423 vs. ExPASy TrEMBL
Match: A0A6J1G4Z1 (calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=1)

HSP 1 Score: 264 bits (674), Expect = 9.64e-87
Identity = 144/215 (66.98%), Postives = 174/215 (80.93%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAW---LKQSPT----KPAAVAA-AVAPPQP 60
           +S+MN+L LAVLFVAG VNFL+YFPTKRF+AW   +K SP     KP AV   + AP + 
Sbjct: 2   ESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEKK 61

Query: 61  PTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEF 120
            +E++K+F TFDKN DG+IT+KEL ESL+N    I EREAEE+ +GVDENGDGLIDFEEF
Sbjct: 62  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 121

Query: 121 CVLAEKL--------RKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLK 180
           CVL EKL        R+ ++E+E++D++ELREAFGVFDKDKDGVISVEELGLVLSCLG+ 
Sbjct: 122 CVLGEKLVMGFEEKQRRVEDEDEDEDEDELREAFGVFDKDKDGVISVEELGLVLSCLGMN 181

Query: 181 EGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
           EGK  ENC+EMI+KVDLDGDGMVNF+EFKRMMR+G
Sbjct: 182 EGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSG 216

[Close]

BLAST of MC08g2423 vs. ExPASy TrEMBL
Match: A0A1S3C5X4 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1)

HSP 1 Score: 238 bits (607), Expect = 1.62e-76
Identity = 131/217 (60.37%), Postives = 161/217 (74.19%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWL------------KQSPTKPAAVAAAVA 60
           + + N LLL+VLFVAG VNFL+YFPTKRF+AW             K +P +P        
Sbjct: 2   EPIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIPHFKSTPLQPPPPPPPPP 61

Query: 61  PPQPPT--ELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGL 120
           PP PP+  EL+K+F TFDKN+DG+IT+KEL ESL++    I E++AEE+ + VDENGDGL
Sbjct: 62  PPPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDGL 121

Query: 121 IDFEEFCVLAEKLRKEKEEEEE----DDDEELREAFGVFDKDKDGVISVEELGLVLSCLG 180
           IDFEEFCVL EKL    EE ++    DD+E L++AFGVFDKD DG+ISVEEL LVL  LG
Sbjct: 122 IDFEEFCVLGEKLLMGFEENKKTSVGDDEEGLKDAFGVFDKDSDGLISVEELSLVLCSLG 181

Query: 181 LKEGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
           + EGK+ ENC+EMIRKVDLDGDGMVNF+EFK+MMRNG
Sbjct: 182 MNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNG 218

[Close]

BLAST of MC08g2423 vs. ExPASy TrEMBL
Match: A0A0A0LL19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1)

HSP 1 Score: 236 bits (601), Expect = 1.41e-75
Identity = 127/219 (57.99%), Postives = 160/219 (73.06%), Query Frame = 0


Query: 1   QSMMNILLLAVLFVAGLVNFLVYFPTKRFSAWLK----------------QSPTKPAAVA 60
           + + N LLL+VLFVAG +NFL+YFP+KRFSAW +                Q P  P+   
Sbjct: 2   EPIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQITHFKSTPLQPPPPPSPSP 61

Query: 61  AAVAPPQPPTELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGD 120
           +   PP    E++K+F TFDKN+DG+IT+KEL ESL++    I E++AEE+ +GVDENGD
Sbjct: 62  SPSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENGD 121

Query: 121 GLIDFEEFCVLAEKLRKEKEEEE----EDDDEELREAFGVFDKDKDGVISVEELGLVLSC 180
           GLIDFEEFCVL  KL    EE +    ED+++EL++AFGVFDKD DG+ISVEEL LVL  
Sbjct: 122 GLIDFEEFCVLGGKLMMGFEENKKTSVEDEEDELKDAFGVFDKDSDGLISVEELSLVLCS 181

Query: 181 LGLKEGKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNG 196
           LG+ EGK+ ENC+EMIRKVDLDGDGMVNF+EFK+MMRNG
Sbjct: 182 LGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNG 220

[Close]

BLAST of MC08g2423 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 150.2 bits (378), Expect = 1.7e-36
Identity = 75/147 (51.02%), Postives = 112/147 (76.19%), Query Frame = 0


Query: 53  PTELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEF 112
           PTEL+++F+ FDKN DG IT KEL+E+LR+   +I ++E  ++ E +D NGDG +D +EF
Sbjct: 3   PTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF 62

Query: 113 CVLAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENC 172
             L + +  E++EEEED    ++EAF VFD++ DG I+V+EL  VLS LGLK+GK  ++C
Sbjct: 63  GELYKTIMDEEDEEEED----MKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDC 122

Query: 173 REMIRKVDLDGDGMVNFEEFKRMMRNG 197
           ++MI+KVD+DGDG VN++EF++MM+ G
Sbjct: 123 KKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of MC08g2423 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 145.2 bits (365), Expect = 5.5e-35
Identity = 74/145 (51.03%), Postives = 107/145 (73.79%), Query Frame = 0


Query: 55  ELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEFCV 114
           EL +IF+ FD+N DG IT++EL +SL N   +I +++  ++ E +D NGDG +D EEF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 115 LAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCRE 174
           L + +      EE D++E++REAF VFD+++DG I+VEEL  VL+ LGLK+G+  E+C+ 
Sbjct: 65  LYQTIM-----EERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKR 124

Query: 175 MIRKVDLDGDGMVNFEEFKRMMRNG 197
           MI KVD+DGDGMVNF+EFK+MM+ G
Sbjct: 125 MISKVDVDGDGMVNFKEFKQMMKGG 144

[Close]

BLAST of MC08g2423 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.0 bits (349), Expect = 4.0e-33
Identity = 76/183 (41.53%), Postives = 120/183 (65.57%), Query Frame = 0


Query: 24  FPTKRFSAWL-KQSPTKPAAV----AAAVAPPQ--PPTELRKIFRTFDKNNDGYITRKEL 83
           FP   F   L K SP  P+ +    +++ AP +   P+EL+++F+ FDKN DG IT++EL
Sbjct: 27  FPLSWFDKTLHKNSPPSPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEEL 86

Query: 84  TESLRN---WIAEREAEELFEGVDENGDGLIDFEEFCVLAEKLRKEKEEEEEDDDEELRE 143
            +SL N   +I +++  ++   +D NGDG +D +EF  L   +  E   + E ++E++++
Sbjct: 87  NDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMKD 146

Query: 144 AFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCREMIRKVDLDGDGMVNFEEFKRMM 197
           AF VFD+D DG I+VEEL  V++ LGLK+GK  + C++MI +VD DGDG VN++EF +MM
Sbjct: 147 AFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

[Close]

BLAST of MC08g2423 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 137.1 bits (344), Expect = 1.5e-32
Identity = 73/146 (50.00%), Postives = 103/146 (70.55%), Query Frame = 0


Query: 54  TELRKIFRTFDKNNDGYITRKELTESLRNW---IAEREAEELFEGVDENGDGLIDFEEFC 113
           TEL ++F+ FDK+ DG IT KEL ES +N    I E E  ++ + +D NGDG +D EEF 
Sbjct: 4   TELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFG 63

Query: 114 VLAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCR 173
            L + +    E+E+E  +E+++EAF VFD++ DG I+V+EL  VLS LGLK+GK  E CR
Sbjct: 64  ELYKTIM--VEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 123

Query: 174 EMIRKVDLDGDGMVNFEEFKRMMRNG 197
           +MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 124 KMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of MC08g2423 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 134.8 bits (338), Expect = 7.5e-32
Identity = 71/145 (48.97%), Postives = 102/145 (70.34%), Query Frame = 0


Query: 55  ELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEFCV 114
           EL ++F+ FDKN DG I + EL +  ++    + E E  E+   +D NGDG +D +EF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 115 LAEKLRKEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKEGKMAENCRE 174
           L +++ +EKEEE     E++REAF VFD++ DG I+ EEL  VL+ +GLK+G+  E+C++
Sbjct: 65  LYQEMVEEKEEE-----EDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKK 124

Query: 175 MIRKVDLDGDGMVNFEEFKRMMRNG 197
           MI KVD+DGDGMVNF+EFK+MMR G
Sbjct: 125 MISKVDVDGDGMVNFKEFKQMMRGG 144

The following BLAST results are available for this feature:

BLAST of MC08g2423 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNE7	2.4e-35	51.02	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SRR7	7.8e-34	51.03	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
O22845	5.6e-32	41.53	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q9ZR02	2.1e-31	50.00	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
Q9SU00	1.0e-30	48.97	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

BLAST of MC08g2423 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143005.1	2.42e-131	100.00	calmodulin-like protein 7 [Momordica charantia]	[more]
XP_023545557.1	6.51e-87	67.61	calmodulin-like protein 7 [Cucurbita pepo subsp. pepo]	[more]
XP_022999398.1	1.74e-86	67.92	calmodulin-like protein 7 [Cucurbita maxima]	[more]
XP_022946838.1	1.99e-86	66.98	calmodulin-like protein 7 [Cucurbita moschata]	[more]
KAG6599380.1	5.69e-86	66.51	Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC08g2423 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMI5	1.17e-131	100.00	calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV...	[more]
A0A6J1KJL5	8.43e-87	67.92	calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1	[more]
A0A6J1G4Z1	9.64e-87	66.98	calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=...	[more]
A0A1S3C5X4	1.62e-76	60.37	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1	[more]
A0A0A0LL19	1.41e-75	57.99	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1	[more]

BLAST of MC08g2423 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	1.7e-36	51.02	EF hand calcium-binding protein family	[more]
AT3G07490.1	5.5e-35	51.03	ARF-GAP domain 11	[more]
AT2G43290.1	4.0e-33	41.53	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	1.5e-32	50.00	EF hand calcium-binding protein family	[more]
AT4G12860.1	7.5e-32	48.97	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 106..133
None	No IPR available	GENE3D	1.10.238.10		coord: 28..128 e-value: 8.7E-21 score: 76.4
None	No IPR available	GENE3D	1.10.238.10		coord: 129..195 e-value: 6.3E-22 score: 79.6
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 4..196
None	No IPR available	PANTHER	PTHR10891:SF796	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 4..196
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 168..196 e-value: 4.2E-6 score: 36.3 coord: 88..116 e-value: 3.8E-5 score: 33.1 coord: 130..158 e-value: 1.8E-5 score: 34.2 coord: 55..83 e-value: 0.003 score: 26.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 55..112 e-value: 3.1E-12 score: 46.8 coord: 129..194 e-value: 6.3E-12 score: 45.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 126..161 score: 15.88028
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 164..196 score: 14.373928
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 51..86 score: 14.178661
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..119 score: 11.33333
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 55..110 e-value: 2.49049E-13 score: 60.2541
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 134..194 e-value: 1.58473E-15 score: 66.0321
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 139..151
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 97..109
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 177..189
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 54..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2423.1	MC08g2423.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding