MC08g2367 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2367
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProlyl endopeptidase
LocationMC08: 32489644 .. 32495126 (+)
RNA-Seq ExpressionMC08g2367
SyntenyMC08g2367
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACCAATTTCGCGTAGCCCTCCGGCACCGCTGCAATCATCTTCACGGCGCTCTGCGGCGATGCCTCCACTACAAGGCACCGAAGCCACCGCAGCCACCGTCACCGGCGGCGCCACCGAAGCCCCCAAAGAAGCCGCAGAGCTTCACAATGCACGAGGTCACATGGGAGGACCCGTACAGTTGGATGTCGAGCTTGAACGACAAGGTGGCGATGCGGCATATGGACGTCTATATGGAGCAGGAGGAGAAGTATACGGAAGCTGTAATGGCCGGCACAGAACGCCTCCAGAGTAAGCTCCAGTCGGAAATGGCTTCTCGCTTGGATTTCGATCTCTCAACCCCTCCCATTCGCTGGGGCCCTTGGTATGTGTTTTTTCTTGGTGAACTATTTGCGTTAGTTAATGTTTTTGTTGCCGATGAGAAGCGCTCTCTTTACCTTGATTACTGTTGATCTCTTTGAAGGCGAATGTCAATCGCCGCAGGTTGTACTATCGAAGAGTCGAAGAAGGAAAGCAATATCCTGTTCTCTGCCGCAGATTAGCGAGCTTACATGAAGAATTTATTTCTAACAGATCTCCTTCCGCTGGATTTGATTATGTGTCGGGCAAGAAAATTGAGCAGAAGTTGCTTGATTACAATCAAGAAGCTGAGAGATTCGGAGGTGTTGTTACTTTCTCTGGCATTCCTCTTTTTTCCTTTGGTTTAAGATGTTCTTTTGTGGGGATTCAGGAATATCATCGAGTTTGGGATGACTATGAGAGGGTAAAACGTGCTTTAAGCCGATTAAAGGTACTTTAAAAAATATTATGGTGTTTGGTTTTATAATTAAAAGTGATTTTTAAAATTAAAAAAACACTTAAAAGTCACTTCTAAAGTGTTTGGGGAAGAAGTGTATTCACTTAAAAATACTTTTTTTAAAAGTTGTCTCAAACTCGCTATTAGGCTTTATACATGGGGCAATGAAATAAGTCTATTTCTTGCTGCAGCATGATAGATTTGGTTTGATCTCATATATGAAATGAAGTAATCATCTGCAAGAAGAAGAGATTAGGTTTCTGATTCTTGTTTTCGTGTGACCATATTTAATGCCCACATGTATTTGGAATACGTTGAAACGCATCCTCTTAATGATTATTGCAGGTTATGCCTACGAGGAGCTATCAGAAGTATCTCCAGATCATCGCTTTCTTGCATACACTATGTATGACAAGGACAATGACTACTTCAGATTATCTGTAAAGAATTTGAATTCCGGTTCTTTGTGTAGTAAGCCTCAAGTGGATCGAGTTTCTAATTTGGCATGGGCCAAAGGTGGCCAGGCATTGCTATATGTTGTTACTGATCAAAATAAAAGACCATGTAGGTTGGTAAATTAGTGTCTCCGTGCAGTTGCTCCTAATTAGTGTATTATTTAAAATTTGTTTTTTCCCATTTTGGCTATGGTTGTGAAAATGAAATGGAATGTGCTTGTTTCTGTCCGTTTAGGTTGTATTGTAGCATGATTGGATCAACCGATGACGATATTTTGCTTCTGGAAGAACTGGATGACGATATCCATGTTTATATTAGACACACAAAAGATTTTCATTTTGTTACTGTTAATCGATTCTCTCCCACGTCTTCTAAGGTGTGTATGACATCTGAAGAACTTCATAGTCGATACTTATGTTAGTTGAAATTATGTATTTGGCTCCTAAATATTCTTATATTTACATTTAGTGAATGTTTTTCGCCACCAATTATAACTTGCTTATATTTGTTCTCCCAATTTAGATTGAGTTCGTTTGTGTTGTTTTCTGAACCCGCATTTTAGGTCTTTCTGATTGATGCTGCTGATCCATTATCTGGTATGGTGTTAATTTGGGAGTGTGGAGGTTTGGCCCATTGCATAATGGAACATCACCTGGGAGATCTTTACTTGTTTACGGATGCTGATAAAGGTCATGAAGCAGTAGATTCTCATTACCTTCTTCGTAGTCCCCTTAAGGTTGATTCTACTGCAAGAACATGGGAGGTTGGTTGTGTTCGTTGGGATTTGGTGTCCCTGTTACACTTTATGATCCAAAAACTTCACCATTTCCACGAACATTGTTTTTCGTCTCTCCATCTTGATAAGTCTTATTTCCACACATTTTCTTAAGCAACCGATTTTATGATGTGGTGCAGCATGTATTTATTGAAGACCAAGACTTGGTGATGTTGGATGTTGATTTCAGTCACACACATTTGGTGCTTATACTTAGGGAAGGCCAGAAATTTAGACTCTGTGCTGTTCGTCTACCCTTGCCTGTTGGAAAGGTCTGGTTTAAGTAATGTCAGGACTTTCTATTGCGCTGGTTGCATTATAATATGTGGCAAAGCCCTAGCCTACTGTTATATATTCCTTGACATCTGTCTCTCTCTATGTACCAGGGACCAATCCATCTCAAAGAACTTGAACTACAATTCCTACCTCTTCCTGAGAATGTATCACAAATTTCCCCAGGACCAAATTATGACTTTTATTCATCAACAATGCGATTTACTATTTCATCACCTGTGGTACGTGGATTTCTATTGGTTTCGTCTTCTTAAGATTGTTTCAATGCACATGGTTGGTTACCATTACTGTATAATGGTTGAGGCACGAAGCTTGATGAATATGAATTCTTAATTGCTTTTAATGTTAAAACTTTTGTCTATTATCATATCTTACGTGAGTTTGGGTGGCAGTTTACTTTTTCTTTATGGTTTAAACTAGAAGATAAGAGAACTGCCCTAGTTTACATTTTAAAATTAATCCAGTACATTTGAAACTTGTAACGAGGGTATAGGGAACTTTTCACCATGTACCTTTTTGTCCTGCAGTAAGCATTTGAATACTCGGATTTTATACCAGGATACGACTACTATTCTATTGGTAATGTGCCAGCAAAACTTGGCACAAACATTTATTTTACAGTCGCCTATTATGGTCTATCCTCTATCTTTAGTTATATTATCTTAGCCGCCCTTCTTTTTTAACTCATTACTTCTGGATTTGTGAATTTGTTTTATTATCCCAATTTATGGGTTGTTGTTACCCTTTTGCCTATGTAAAGTTGTCAAGTTCGCTTCTCAACTTGTTTCCTTATACTAAACTTGGGTCTGACTCTATGATATTGAGCTATTATCCTTGATAGCTTTAGCTTTAACAACCTATCCTTTGGAATGTAATGGTTATAGAACTTAACCTCCTTAAACTTCAAGATAAATGTAATAGTAATACTGTCTGACCATTTGCCGCCCATTAATTGTTTTCCGAATTTCAAACAGATGCCTGATGCTGTGGTTGATTATCACCTATCAGATGGAAAATGGAATATCATTCAACAGCAAAGCATTCTTCATGAAAGAACACGAATTCTGTATGGAACAACCTCTACTACAGGAGCATCAGGAAAAATATCTAACGTGTTGGAGGACTCTGTGGGTGAAGGCAACTTGAATGATGATCAGATGTGGAACAGCCTCTCTGAATTTTATGCTTGTGAACGCTTCAATGTGCCATCAAATGATGGGGTTTTGGTTCCTTTAACAGTTGTATACTCTTACAAGTGTAAAAGAGAAAATGACAACCCTGGATTACTACATGTACATGGAGCATATGGTGAGCTACTTGACAAACGGTGGCGCAGTGAGTTGAAAAGCCTTCTCGATCGTGGCTGGGTCATTGCATATGCTGATGTGAGGTTTGTATGCATTTGTACTTCTCTGAATTCCAAATACCTTTGTAGCTGAACTTTTTATTGCTTTGGTGATGATACACAGCTTTCTCCGGTAGAAATTGTTTTAATTCAACATATGATAGTTATCACTTATTTCAGTTACCGTAAAAACTTCTATTTCTCAATTTTGGCATCTGATTTCTTTTTGTTTGCCCCTGTCATATAATTTGATGCTTTGGTTCTAGAGGTGGAGGCGGTGGGGGTAAGAAGTGGCATCATGATGGTAGGCGTGCAAAGAAGTTTAATTCAATTCAAGACTATATTTCATGTGCTAAATTTCTTGCTGAGAGAAATATTATAAATGAGGAGAAGCTTGCTGGTTGGGGCTATAGTGCTGGAGGACTTTTGGTTGCTTCTGCCATCAATCAATGCCCAGAATTATTTCGAGCTGCTATTTTGAAAGTATGCTTCCATCTTTTGCATTATTTCTCTTTCATACTTGTTGCATCTTATGTTACTTCTGAAGTCGGATGAGGGCTTCCCTTTTCTAAGACTTTATTCTTTTTGTATCATCACTTTGTATGTATGTATATGTGTGTGTGTATATATATCTCTCTGCTTGTTTTGAAATAACTTCTCTGTGAACAGTGTGGCTAATTTTAGTGCTTGGAGGCATGTTTGGGAGTGAAAAGTCAAAATCATTTTCTCCATTTGTAAACACTAGGATATGTACTTTTGTCATTGTCAAAATCACACCTATTTAAAATAATAATGATATACAAATGATTTTAGAAAATGGTGAAAGGTTTTTGGCTCAAACATGCCTTTTATCCATTTTGTTTGACCAAATCTTTCTTTTTGTCGCAGGTTCCATTTCTAGATCCAATAAGCACACTCCTTCATCCCATTATACCACTAACAGCTGCTGACTATGAAGAATTTGGATATCCTGGGAATGTAGATGATTTTCATGCAATACGCAGGTATTCTCCCTACGATAACATACAGAAGGATGTCGCCTACCCTGCTGTTTTGATAACCTCAACCTTCAATACCCGGTAATTGTCATTCTTTTTCTGCTTGTCTAAAGTTCATGTCATTGTAAAGTCCATGCTTCTTTCTTAAACAAGAAAAATGTTCTGCATAGGCGTTCAGGATGTTGCTTTTGGCTGAACTAGAATTAGAAGACTAGAAGTTAGCTGATAATAAAAGAAAAGAGGAAGCCAAGTATTTATCACTCAGGACATTTCAAATTTCAGCCCATTCTAGTTTTCTAGCTTTTAAATAGATCAACTAGTCGTTTCATTTACTCATCAGGAAATATTGTTCCTTGCCCAGTCATTATGTTATGTTCAATCTTCTTATTTGTGTTATACAGATTTGGAGTATGGGAAGCTGCAAAATGGATTGCTCGAGTCCGTGATTTCAGTATTTACGATCCGAAGCGTCCAGTAATTCTCAATTTAACAACAGACATAGTAGAGGAAAACAGGTATTTGCACTGTAAAGAATCAGCTTTAGAGACTGCATTTCTTATGAAGGTGATGGAATCCTAGGCTGCACTACCTAGAACTGCAGTTAAAATTAAGATCTATACATGCAAGTTGTGGAGACGATAATTAGGATAGATTTAGATTAGATTAGGTTAGGCGAGGGTAAAATTCATCAAGGGTTTTAAATAGTCAAGAAATCTACCCTCGTTTAGGTAAATTATTAGAATGGGTTATTTATTTTTCAAAATAATGGAACGGAAATAATGTATTGTAATGAATCGTTGTGATAAGTTTTTCTTTGT

mRNA sequence

ATGAACCAATTTCGCGTAGCCCTCCGGCACCGCTGCAATCATCTTCACGGCGCTCTGCGGCGATGCCTCCACTACAAGGCACCGAAGCCACCGCAGCCACCGTCACCGGCGGCGCCACCGAAGCCCCCAAAGAAGCCGCAGAGCTTCACAATGCACGAGGTCACATGGGAGGACCCGTACAGTTGGATGTCGAGCTTGAACGACAAGGTGGCGATGCGGCATATGGACGTCTATATGGAGCAGGAGGAGAAGTATACGGAAGCTGTAATGGCCGGCACAGAACGCCTCCAGAGTAAGCTCCAGTCGGAAATGGCTTCTCGCTTGGATTTCGATCTCTCAACCCCTCCCATTCGCTGGGGCCCTTGGTTGTACTATCGAAGAGTCGAAGAAGGAAAGCAATATCCTGTTCTCTGCCGCAGATTAGCGAGCTTACATGAAGAATTTATTTCTAACAGATCTCCTTCCGCTGGATTTGATTATGTGTCGGGCAAGAAAATTGAGCAGAAGTTGCTTGATTACAATCAAGAAGCTGAGAGATTCGGAGGTTATGCCTACGAGGAGCTATCAGAAGTATCTCCAGATCATCGCTTTCTTGCATACACTATGTATGACAAGGACAATGACTACTTCAGATTATCTGTAAAGAATTTGAATTCCGGTTCTTTGTGTAGTAAGCCTCAAGTGGATCGAGTTTCTAATTTGGCATGGGCCAAAGGTGGCCAGGCATTGCTATATGTTGTTACTGATCAAAATAAAAGACCATGTAGGTTGTATTGTAGCATGATTGGATCAACCGATGACGATATTTTGCTTCTGGAAGAACTGGATGACGATATCCATGTTTATATTAGACACACAAAAGATTTTCATTTTGTTACTGTTAATCGATTCTCTCCCACGTCTTCTAAGGTCTTTCTGATTGATGCTGCTGATCCATTATCTGGTATGGTGTTAATTTGGGAGTGTGGAGGTTTGGCCCATTGCATAATGGAACATCACCTGGGAGATCTTTACTTGTTTACGGATGCTGATAAAGGTCATGAAGCAGTAGATTCTCATTACCTTCTTCGTAGTCCCCTTAAGGTTGATTCTACTGCAAGAACATGGGAGCATGTATTTATTGAAGACCAAGACTTGGTGATGTTGGATGTTGATTTCAGTCACACACATTTGGTGCTTATACTTAGGGAAGGCCAGAAATTTAGACTCTGTGCTGTTCGTCTACCCTTGCCTGTTGGAAAGGGACCAATCCATCTCAAAGAACTTGAACTACAATTCCTACCTCTTCCTGAGAATGTATCACAAATTTCCCCAGGACCAAATTATGACTTTTATTCATCAACAATGCGATTTACTATTTCATCACCTGTGATGCCTGATGCTGTGGTTGATTATCACCTATCAGATGGAAAATGGAATATCATTCAACAGCAAAGCATTCTTCATGAAAGAACACGAATTCTGTATGGAACAACCTCTACTACAGGAGCATCAGGAAAAATATCTAACGTGTTGGAGGACTCTGTGGGTGAAGGCAACTTGAATGATGATCAGATGTGGAACAGCCTCTCTGAATTTTATGCTTGTGAACGCTTCAATGTGCCATCAAATGATGGGGTTTTGGTTCCTTTAACAGTTGTATACTCTTACAAGTGTAAAAGAGAAAATGACAACCCTGGATTACTACATGTACATGGAGCATATGGTGAGCTACTTGACAAACGGTGGCGCAGTGAGTTGAAAAGCCTTCTCGATCGTGGCTGGGTCATTGCATATGCTGATGTGAGAGGTGGAGGCGGTGGGGGTAAGAAGTGGCATCATGATGGTAGGCGTGCAAAGAAGTTTAATTCAATTCAAGACTATATTTCATGTGCTAAATTTCTTGCTGAGAGAAATATTATAAATGAGGAGAAGCTTGCTGGTTGGGGCTATAGTGCTGGAGGACTTTTGGTTGCTTCTGCCATCAATCAATGCCCAGAATTATTTCGAGCTGCTATTTTGAAAGTTCCATTTCTAGATCCAATAAGCACACTCCTTCATCCCATTATACCACTAACAGCTGCTGACTATGAAGAATTTGGATATCCTGGGAATGTAGATGATTTTCATGCAATACGCAGGTATTCTCCCTACGATAACATACAGAAGGATGTCGCCTACCCTGCTGTTTTGATAACCTCAACCTTCAATACCCGATTTGGAGTATGGGAAGCTGCAAAATGGATTGCTCGAGTCCGTGATTTCAGTATTTACGATCCGAAGCGTCCAGTAATTCTCAATTTAACAACAGACATAGTAGAGGAAAACAGGTATTTGCACTGTAAAGAATCAGCTTTAGAGACTGCATTTCTTATGAAGGTGATGGAATCCTAGGCTGCACTACCTAGAACTGCAGTTAAAATTAAGATCTATACATGCAAGTTGTGGAGACGATAATTAGGATAGATTTAGATTAGATTAGGTTAGGCGAGGGTAAAATTCATCAAGGGTTTTAAATAGTCAAGAAATCTACCCTCGTTTAGGTAAATTATTAGAATGGGTTATTTATTTTTCAAAATAATGGAACGGAAATAATGTATTGTAATGAATCGTTGTGATAAGTTTTTCTTTGT

Coding sequence (CDS)

ATGAACCAATTTCGCGTAGCCCTCCGGCACCGCTGCAATCATCTTCACGGCGCTCTGCGGCGATGCCTCCACTACAAGGCACCGAAGCCACCGCAGCCACCGTCACCGGCGGCGCCACCGAAGCCCCCAAAGAAGCCGCAGAGCTTCACAATGCACGAGGTCACATGGGAGGACCCGTACAGTTGGATGTCGAGCTTGAACGACAAGGTGGCGATGCGGCATATGGACGTCTATATGGAGCAGGAGGAGAAGTATACGGAAGCTGTAATGGCCGGCACAGAACGCCTCCAGAGTAAGCTCCAGTCGGAAATGGCTTCTCGCTTGGATTTCGATCTCTCAACCCCTCCCATTCGCTGGGGCCCTTGGTTGTACTATCGAAGAGTCGAAGAAGGAAAGCAATATCCTGTTCTCTGCCGCAGATTAGCGAGCTTACATGAAGAATTTATTTCTAACAGATCTCCTTCCGCTGGATTTGATTATGTGTCGGGCAAGAAAATTGAGCAGAAGTTGCTTGATTACAATCAAGAAGCTGAGAGATTCGGAGGTTATGCCTACGAGGAGCTATCAGAAGTATCTCCAGATCATCGCTTTCTTGCATACACTATGTATGACAAGGACAATGACTACTTCAGATTATCTGTAAAGAATTTGAATTCCGGTTCTTTGTGTAGTAAGCCTCAAGTGGATCGAGTTTCTAATTTGGCATGGGCCAAAGGTGGCCAGGCATTGCTATATGTTGTTACTGATCAAAATAAAAGACCATGTAGGTTGTATTGTAGCATGATTGGATCAACCGATGACGATATTTTGCTTCTGGAAGAACTGGATGACGATATCCATGTTTATATTAGACACACAAAAGATTTTCATTTTGTTACTGTTAATCGATTCTCTCCCACGTCTTCTAAGGTCTTTCTGATTGATGCTGCTGATCCATTATCTGGTATGGTGTTAATTTGGGAGTGTGGAGGTTTGGCCCATTGCATAATGGAACATCACCTGGGAGATCTTTACTTGTTTACGGATGCTGATAAAGGTCATGAAGCAGTAGATTCTCATTACCTTCTTCGTAGTCCCCTTAAGGTTGATTCTACTGCAAGAACATGGGAGCATGTATTTATTGAAGACCAAGACTTGGTGATGTTGGATGTTGATTTCAGTCACACACATTTGGTGCTTATACTTAGGGAAGGCCAGAAATTTAGACTCTGTGCTGTTCGTCTACCCTTGCCTGTTGGAAAGGGACCAATCCATCTCAAAGAACTTGAACTACAATTCCTACCTCTTCCTGAGAATGTATCACAAATTTCCCCAGGACCAAATTATGACTTTTATTCATCAACAATGCGATTTACTATTTCATCACCTGTGATGCCTGATGCTGTGGTTGATTATCACCTATCAGATGGAAAATGGAATATCATTCAACAGCAAAGCATTCTTCATGAAAGAACACGAATTCTGTATGGAACAACCTCTACTACAGGAGCATCAGGAAAAATATCTAACGTGTTGGAGGACTCTGTGGGTGAAGGCAACTTGAATGATGATCAGATGTGGAACAGCCTCTCTGAATTTTATGCTTGTGAACGCTTCAATGTGCCATCAAATGATGGGGTTTTGGTTCCTTTAACAGTTGTATACTCTTACAAGTGTAAAAGAGAAAATGACAACCCTGGATTACTACATGTACATGGAGCATATGGTGAGCTACTTGACAAACGGTGGCGCAGTGAGTTGAAAAGCCTTCTCGATCGTGGCTGGGTCATTGCATATGCTGATGTGAGAGGTGGAGGCGGTGGGGGTAAGAAGTGGCATCATGATGGTAGGCGTGCAAAGAAGTTTAATTCAATTCAAGACTATATTTCATGTGCTAAATTTCTTGCTGAGAGAAATATTATAAATGAGGAGAAGCTTGCTGGTTGGGGCTATAGTGCTGGAGGACTTTTGGTTGCTTCTGCCATCAATCAATGCCCAGAATTATTTCGAGCTGCTATTTTGAAAGTTCCATTTCTAGATCCAATAAGCACACTCCTTCATCCCATTATACCACTAACAGCTGCTGACTATGAAGAATTTGGATATCCTGGGAATGTAGATGATTTTCATGCAATACGCAGGTATTCTCCCTACGATAACATACAGAAGGATGTCGCCTACCCTGCTGTTTTGATAACCTCAACCTTCAATACCCGATTTGGAGTATGGGAAGCTGCAAAATGGATTGCTCGAGTCCGTGATTTCAGTATTTACGATCCGAAGCGTCCAGTAATTCTCAATTTAACAACAGACATAGTAGAGGAAAACAGGTATTTGCACTGTAAAGAATCAGCTTTAGAGACTGCATTTCTTATGAAGGTGATGGAATCCTAG

Protein sequence

MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPYSWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGGQALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPLKVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLKELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGVLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAYPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALETAFLMKVMES
Homology
BLAST of MC08g2367 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 7.1e-70
Identity = 202/753 (26.83%), Postives = 341/753 (45.29%), Query Frame = 0

Query: 40  PKPPKKPQSFTMHEVTWEDPYSWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSK 99
           P   + P    +H    ED Y W+   ++   ++    Y+E+E +Y   +M   +    +
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKDRDNTEVIQ----YLEEENRYYHEIMRPLQEQTEQ 63

Query: 100 LQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFD 159
           +   M  R+       P++ G + YY R+++ KQYP+  R+ A+                
Sbjct: 64  IYESMVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYARKQAASRA------------- 123

Query: 160 YVSGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNS 219
            +     E+ +LD N+ AE    Y    +  ++ DH  LAY       D + + +K+LN+
Sbjct: 124 -LLQDATEEVVLDLNELAEE-DDYLSVTVQRMTTDHSRLAYLENRDGTDRYTIYIKDLNT 183

Query: 220 GSLCSK--PQVDRVSNLAWAKGGQALLYVVTDQNKRPCRLYCSMIGS-TDDDILLLEELD 279
           G L S   P V    ++ W + G  + Y   D+++RPC+L+   +GS  + D L+ EE D
Sbjct: 184 GELLSDRVPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVESDELIFEEKD 243

Query: 280 DDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGD 339
           D   ++I  ++   F+ V   S T+S++ +ID   PLS + L+ E        +EH   D
Sbjct: 244 DTFTLFISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGILYDVEHWEDD 303

Query: 340 LYLFTDADKGHEAVDSHYLLRSPLKVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILR 399
           L + T     +E   +  LLR PL  D +++   +V   +++  + ++      L++  R
Sbjct: 304 LLILT-----NEGALNFQLLRCPLN-DLSSKV--NVVEYNEERYLQEMYPFRDKLLIAGR 363

Query: 400 EGQKFRLCAVRLPLPVGKGPIHLKELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSP 459
           E    ++  V           H  EL+                          + +   P
Sbjct: 364 ENGLTQIWVV-----------HDGELQ--------------------------QISWDEP 423

Query: 460 VMPDAVVDYHLSDGKWNIIQQQSILHERTRILYGTTSTTGASGKISNV-LEDSVGEGNLN 519
           +   AV+     D    +IQ +S+L  +T   +G    TG    +    +          
Sbjct: 424 LYTVAVLSEQSYDTNEVLIQYESLLTPKT--TFGLNLQTGEKQCLQVAPVSGEYDRSQFR 483

Query: 520 DDQMWNSLSEFYACERFNVPSNDGVLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKR 579
            +Q+W                  GV VP+T VY          P +L+ +G+YG   D R
Sbjct: 484 QEQLW-------------ATGRSGVKVPMTAVYLEGALDNGPAPLILYGYGSYGSNSDPR 543

Query: 580 WRSELKSLLDRGWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIIN 639
           +      LL++G V   A VRGG   G+ W+ DG+   K N+  D+I+ AK L ++N  +
Sbjct: 544 FDPYRLPLLEKGIVFVTAQVRGGSEMGRGWYEDGKMQNKRNTFTDFIAAAKHLIDQNYTS 603

Query: 640 EEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGY 699
             K+A  G SAGGLLV +  N   ELF+  +  VPF+D ++T+L   IPLT  +++E+G 
Sbjct: 604 PTKMAARGGSAGGLLVGAVANMAGELFKVIVPAVPFVDVVTTMLDTSIPLTTLEWDEWGD 663

Query: 700 PGNVDDFHAIRRYSPYDNIQ-KDVAYPAVLITSTFN-TRFGVWEAAKWIARVRDFSIYDP 759
           P   +D+  ++ YSPYDN++ KD  YP + IT+  N  R G +E AKW+AR+R     + 
Sbjct: 664 PRKQEDYFYMKSYSPYDNVEAKD--YPHMYITTGINDPRVGYFEPAKWVARLRAVKTDNN 675

Query: 760 KRPVILNL-TTDIVEENRYLHCKESALETAFLM 786
              +  N+      +  R+ H KE+A   AF++
Sbjct: 724 TLVMKTNMGAGHFGKSGRFNHLKEAAESYAFIL 675

BLAST of MC08g2367 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.5e-69
Identity = 209/759 (27.54%), Postives = 339/759 (44.66%), Query Frame = 0

Query: 37  AAPPKPPKKPQSF-TMHEVTWEDPYSWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTER 96
           A PP   KKP      H     D Y W+   +DK   + M  Y+  E  YT+AVMA  + 
Sbjct: 26  ATPPDVAKKPHVVKAPHGAERNDEYYWLR--DDKRENKEMLAYLNAENAYTDAVMAPLKP 85

Query: 97  LQSKLQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPS 156
           L+ KL  E+ +R+  D ++ P R   W YY R   GK YPV  RR      + +S ++ +
Sbjct: 86  LEDKLYDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARRKDGPGVDAVSIQAAN 145

Query: 157 AGFDYVSGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVK 216
           A  D+      EQ LLD N        Y   +  EVS D+R LAY         + +  K
Sbjct: 146 AAGDFAG----EQVLLDVNALGAGKDYYNVGDY-EVSQDNRLLAYADDTNGRRQYTIRFK 205

Query: 217 NLNSGSLCSKPQVDRVSNLAWAKGGQALLYVVTD-QNKRPCRLYCSMIGS-TDDDILLLE 276
           NL++G L      +   NL W+  G+ L YV  D +     R+   ++G+    D L+ E
Sbjct: 206 NLDTGELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTPASQDALVYE 265

Query: 277 ELDDDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSGMVLIWECGGLAHCIMEHH 336
           E DD  ++ I  ++D  F+ ++  S  SS++    AA P    VL      + +     H
Sbjct: 266 EEDDSFYMGIGRSRDDKFICISVESTVSSEMRCTPAASPGVFTVLAPRERDVEY--QADH 325

Query: 337 LGDLYLF-TDADKGHEAVDSHYLLRSPLKVDSTAR-TWEHVFIEDQDLVMLDVDFSHTHL 396
           LGD ++  T+AD       +  ++ +P   DST+R  W+       D+ +   +      
Sbjct: 326 LGDRWVIRTNAD----GATNFKIVTAP--TDSTSRKDWKDWVAHRDDVFVEGFELFDGFS 385

Query: 397 VLILREGQKFRLCAVRLPLPVGKGPIHLKELELQFLPLPENVSQISPGPNYDFYSSTMRF 456
           V+  R      L  ++             +    ++   E+   +    N +  +  +R+
Sbjct: 386 VVAERANALESLRVIK------------ADGSSDYVKADESAYSMGLSANPETGTDWLRY 445

Query: 457 TISSPVMPDAVVDYHLSDGKWNIIQQQSILHERTRILYGTTSTTGASGKISNVLEDSVGE 516
           + +S   P    + +   G+   ++QQ +        Y  +                   
Sbjct: 446 SYTSMTTPATTYEINTKTGERRQLKQQPVPG------YDASK------------------ 505

Query: 517 GNLNDDQMWNSLSEFYACERFNVPSNDG-VLVPLTVVYSYKCKRENDNPGLLHVHGAYGE 576
                          Y  ER   P+ DG   +P+T+VY     R+   P L + +G+YG 
Sbjct: 506 ---------------YVTERVWAPARDGKTKIPVTLVYRKDVARDGKAPMLQYAYGSYGA 565

Query: 577 LLDKRWRSELKSLLDRGWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLAE 636
            +D  +     SLLDRG V A A +RGG   G+ W+ DG+   K N+  D+I    +L +
Sbjct: 566 SMDPNFSITNVSLLDRGVVYALAHIRGGQEMGRAWYDDGKLYNKINTFTDFIDVTDYLVK 625

Query: 637 RNIINEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPLTAADY 696
                ++++A  G SAGGLL+ +  N  PE ++  +  VPF+D ++T+L P IPLT  +Y
Sbjct: 626 EGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVILTLVPFVDVVTTMLDPTIPLTTNEY 685

Query: 697 EEFGYPGNVDDFHAIRRYSPYDNIQKDVAYPAVLI-TSTFNTRFGVWEAAKWIARVRDFS 756
           +E+G P     +  I  YSPYDN+Q   AYPA+ + T  ++++   WE AK++AR+RD +
Sbjct: 686 DEWGNPEEKGYYDYILTYSPYDNLQAK-AYPAMFVGTGLWDSQVQYWEPAKYVARLRDLN 715

Query: 757 IYDPKRPVILNLTTDI---VEENRYLHCKESALETAFLM 786
               K PV+     +     +  R+   +E A   AF++
Sbjct: 746 --TGKGPVVFRTNMEAGHGGKSGRFRQYRERAEMFAFML 715

BLAST of MC08g2367 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 217.6 bits (553), Expect = 5.0e-55
Identity = 188/735 (25.58%), Postives = 325/735 (44.22%), Query Frame = 0

Query: 78  YMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVL 137
           Y++QE  Y   VMA  + LQ ++  E+  R+     + P     + Y    E G +Y + 
Sbjct: 39  YLQQENSYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIY 98

Query: 138 CRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRF 197
            +R ++  EE+                   + LLD N+ A     Y+   ++ ++PD+  
Sbjct: 99  -QRQSAFSEEWDE----------------WETLLDANKRAAHSEFYSMGGMA-ITPDNTI 158

Query: 198 LAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGGQALLYVVTDQ-NKRPCR 257
           +A          + +  +NL +G+   +   +   +  WA       YV        P +
Sbjct: 159 MALAEDFLSRRQYGIRFRNLETGNWYPELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQ 218

Query: 258 LYCSMIGS-TDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSG 317
           ++   IG+    D L+ EE DD  +V +  T   H+V ++  S T+S+V L+DA      
Sbjct: 219 VWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDA------ 278

Query: 318 MVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPLKVDSTART------- 377
                          E    + ++F    K HE    HY  R  L+ +   +        
Sbjct: 279 ---------------EMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTR 338

Query: 378 ------WEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLKEL 437
                 WE + I  ++ +ML+  F+     L++ E Q+  L ++R         I+ K  
Sbjct: 339 MRDEQQWEEL-IPPRENIMLE-GFTLFTDWLVVEERQR-GLTSLR--------QINRKTR 398

Query: 438 ELQFLPL--PENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI 497
           E+  +    P  V+ I+  P  +  ++ +R+  SS   PD + +  +  G+  +++Q  +
Sbjct: 399 EVIGIAFDDPAYVTWIAYNPEPE--TARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEV 458

Query: 498 LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV 557
                                           N   + +W             + + DGV
Sbjct: 459 --------------------------PGFYAANYRSEHLW-------------IVARDGV 518

Query: 558 LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG 617
            VP+++VY  K  R+  NP L++ +G+YG  +D  +     SLLDRG+V A   VRGGG 
Sbjct: 519 EVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGE 578

Query: 618 GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE 677
            G++W+ DG+  KK N+  DY+     L +    +       G SAGG+L+  AINQ PE
Sbjct: 579 LGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE 638

Query: 678 LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY 737
           LF   I +VPF+D ++T+L   IPLT  ++EE+G P +   +  ++ YSPYDN+    AY
Sbjct: 639 LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQ-AY 677

Query: 738 PAVLITS-TFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDI-----VEENRYLHC 790
           P +L+T+   +++   WE AKW+A++R+    D     +L L TD+      +  R+   
Sbjct: 699 PHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDH----LLLLCTDMDSGHGGKSGRFKSY 677

BLAST of MC08g2367 vs. ExPASy Swiss-Prot
Match: Q4J6C6 (Prolyl endopeptidase-like OS=Homo sapiens OX=9606 GN=PREPL PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.5e-51
Identity = 173/655 (26.41%), Postives = 279/655 (42.60%), Query Frame = 0

Query: 80  EQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVLCR 139
           ++ EK     M   E++++KL+++   + ++++    ++ G ++YY   +EG      C 
Sbjct: 80  QENEKPLPENMDAFEKVRTKLETQ--PQEEYEIINVEVKHGGFVYY---QEG------CC 139

Query: 140 RLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRFLA 199
            + S  EE   N +    F+ +   K++Q  +D                  V+PD +++A
Sbjct: 140 LVRSKDEE-ADNDNYEVLFN-LEELKLDQPFID---------------CIRVAPDEKYVA 199

Query: 200 YTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKG--GQALLYVVTDQNKRPCRL 259
             +  +D++     +  L S     +     VS+  W K    + +L+    +N R   +
Sbjct: 200 AKIRTEDSEASTCVIIKL-SDQPVMEASFPNVSSFEWVKDEEDEDVLFYTFQRNLRCHDV 259

Query: 260 YCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSGMV 319
           Y +  G    +     E D    V++  TKD  F+T+N  + T+S+V+LID   P    V
Sbjct: 260 YRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTINIMNKTTSEVWLIDGLSPWDPPV 319

Query: 320 LIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPLKVDSTARTWEHVFIEDQ 379
           LI +        +EH   +LY+ T+  +  E     + L            W+  F   +
Sbjct: 320 LIQKRIHGVLYYVEHRDDELYILTNVGEPTE-----FKLMRTAADTPAIMNWDLFFTMKR 379

Query: 380 DLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLKELELQFLPLPENVSQIS 439
           +  ++D+D    H VL L+      +             I L +  ++ L LP       
Sbjct: 380 NTKVIDLDMFKDHCVLFLKHSNLLYV-----------NVIGLADDSVRSLKLPPWACGFI 439

Query: 440 PGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSILHERTRILYGTTSTTGA 499
              N D       F + SP+ P     Y  ++GK                L+  T     
Sbjct: 440 MDTNSD--PKNCPFQLCSPIRPPKYYTYKFAEGK----------------LFEETGHEDP 499

Query: 500 SGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGVLVPLTVVYSYKCKREND 559
             K S VL                         R    S DG LVP+TV +    +    
Sbjct: 500 ITKTSRVL-------------------------RLEAKSKDGKLVPMTVFHKTDSEDLQK 559

Query: 560 NPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGGGGKKWHHDGRRAKKFNS 619
            P L+HV+GAYG  L   +R E + L+D GW++AY  VRGGG  G +WH DGR  KK N 
Sbjct: 560 KPLLVHVYGAYGMDLKMNFRPERRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNG 619

Query: 620 IQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPIST 679
           + D  +C K L  +            +SAGG+L  +  N  PEL RA  L+ PFLD ++T
Sbjct: 620 LADLEACIKTLHGQGFSQPSLTTLTAFSAGGVLAGALCNSNPELVRAVTLEAPFLDVLNT 644

Query: 680 LLHPIIPLTAADYEEFGYPGNVDDFHA--IRRYSPYDNIQKDVAYPAVLITSTFN 731
           ++   +PLT  + EE+G P + D+ H   I+RY PY NI K   YP++ IT+  N
Sbjct: 680 MMDTTLPLTLEELEEWGNPSS-DEKHKNYIKRYCPYQNI-KPQHYPSIHITAYEN 644

BLAST of MC08g2367 vs. ExPASy Swiss-Prot
Match: Q8C167 (Prolyl endopeptidase-like OS=Mus musculus OX=10090 GN=Prepl PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.5e-51
Identity = 159/545 (29.17%), Postives = 241/545 (44.22%), Query Frame = 0

Query: 191 VSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKG--GQALLYVVT 250
           V+PD +++A  +  +D++   L V  L S     +     VS+  W K    + +L+   
Sbjct: 161 VAPDEKYVAAKIRTEDSETSTLVVVKL-SDQPVMEASFPNVSSFEWVKDEEDEDVLFYTF 220

Query: 251 DQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPTSSKVFLID 310
            +N R   +Y +  G    +     E D    V++  TKD  F+T+N  + T+S+V+LID
Sbjct: 221 QRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTLNIMNKTTSEVWLID 280

Query: 311 AADPLSGMVLIWE-CGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPLKVDSTAR 370
              P    VLI +   G+ + + EH   +LY+ T+  +  E      L+R+     +   
Sbjct: 281 GLSPWDPPVLIQKRIHGMLYYV-EHRDDELYILTNVGEPTE----FKLMRTAADAPA-IM 340

Query: 371 TWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLKELELQFL 430
            W+  F   ++  ++D+D    H VL L+      +             I L +  ++ L
Sbjct: 341 NWDLFFTMKRNTKVVDLDMFKDHCVLFLKHSNLLYV-----------NVIGLADDSVRSL 400

Query: 431 PLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSILHERTRI 490
            LP          N D       F + SP+ P     Y  ++GK                
Sbjct: 401 KLPPWACGFIMDTNSD--PKNCPFQLCSPIRPPKYYTYKFAEGK---------------- 460

Query: 491 LYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGVLVPLTVV 550
           L+  T       K S VL                         R    S DG LVP+TV 
Sbjct: 461 LFEETGHEDPITKTSRVL-------------------------RIEAKSKDGKLVPMTVF 520

Query: 551 YSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGGGGKKWHH 610
           +    +     P L+HV+GAYG  L   +R E + L+D GW++AY  VRGGG  G +WH 
Sbjct: 521 HKTDSEDLQRKPLLVHVYGAYGMDLKMNFRPEKRVLVDDGWILAYCHVRGGGELGLQWHA 580

Query: 611 DGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPELFRAAIL 670
           DGR  KK N + D ++C K L  +            +SAGG+LV +  N  PEL RA  L
Sbjct: 581 DGRLTKKLNGLADLVACIKTLHSQGFSQPSLTTLSAFSAGGVLVGALCNSKPELLRAVTL 640

Query: 671 KVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHA--IRRYSPYDNIQKDVAYPAVLI 730
           + PFLD ++T+L   +PLT  + EE+G P + D+ H   I+RY P  NI K   YP+V I
Sbjct: 641 EAPFLDVLNTMLDTTLPLTLEELEEWGNPSS-DEKHKNYIKRYCPCQNI-KPQHYPSVHI 642

BLAST of MC08g2367 vs. NCBI nr
Match: XP_022142957.1 (uncharacterized protein LOC111012951 [Momordica charantia])

HSP 1 Score: 1628 bits (4215), Expect = 0.0
Identity = 790/790 (100.00%), Postives = 790/790 (100.00%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY
Sbjct: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT
Sbjct: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL
Sbjct: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK 420
           KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK
Sbjct: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK 420

Query: 421 ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI 480
           ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI
Sbjct: 421 ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI 480

Query: 481 LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV 540
           LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV
Sbjct: 481 LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV 540

Query: 541 LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG 600
           LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG
Sbjct: 541 LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG 600

Query: 601 GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE 660
           GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE
Sbjct: 601 GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE 660

Query: 661 LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY 720
           LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY
Sbjct: 661 LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY 720

Query: 721 PAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALE 780
           PAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALE
Sbjct: 721 PAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALE 780

Query: 781 TAFLMKVMES 790
           TAFLMKVMES
Sbjct: 781 TAFLMKVMES 790

BLAST of MC08g2367 vs. NCBI nr
Match: XP_038889448.1 (dipeptidyl aminopeptidase BI [Benincasa hispida])

HSP 1 Score: 1491 bits (3859), Expect = 0.0
Identity = 718/791 (90.77%), Postives = 757/791 (95.70%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MN+ R ALRHR  +LHGALRRCLHYKAPK PQPPSPAAPPKPPKKPQSF+MHE+TWEDPY
Sbjct: 1   MNRLRAALRHRRTNLHGALRRCLHYKAPKSPQPPSPAAPPKPPKKPQSFSMHELTWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRL FDLSTPP+RWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLAFDLSTPPLRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQYPVLCRRLASLHEEFISN+SPSAGFDYVSGKKIEQKL+DYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNKSPSAGFDYVSGKKIEQKLIDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAY+MYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYSMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           QALLYVVTDQNKRPCRLYCS+IGSTD+DILLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QALLYVVTDQNKRPCRLYCSLIGSTDEDILLLEEPDDDVHVYIRHTKDFRFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLI+AADP+SGM LIWEC GLAHCI+EHHLGDLYLFTDA KGHE VDSHYLLRSPL
Sbjct: 301 SSKVFLINAADPVSGMKLIWECEGLAHCIVEHHLGDLYLFTDASKGHEPVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
           KVDS  RTWEHVF++D DLV++DVDFSH HLVLILR G KFRLCAVRLPLPVG KGPI+L
Sbjct: 361 KVDSIPRTWEHVFVDDPDLVIVDVDFSHMHLVLILRGGGKFRLCAVRLPLPVGGKGPINL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELELQFLPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELELQFLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGT S+ G SGK+SN LE+SV E N +DDQMWNSLSE+YACE FNV S+DG
Sbjct: 481 ILHERTRILYGTASSAGGSGKVSNALENSVDEANFDDDQMWNSLSEYYACEHFNVSSDDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VL+PLT+VYSYKCK+EN+NPGLL+VHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLIPLTIVYSYKCKKENENPGLLNVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWH DGRR KKFNS+QDYISCAKFLAER I+NEEKLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHQDGRRLKKFNSVQDYISCAKFLAERKIVNEEKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA 720
           ELFRAAILKVPFLDPI+TLLHPIIPLT ADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA
Sbjct: 661 ELFRAAILKVPFLDPINTLLHPIIPLTPADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA 720

Query: 721 YPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESAL 780
           YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILNLTTDIVEENRYLHCKESAL
Sbjct: 721 YPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNLTTDIVEENRYLHCKESAL 780

Query: 781 ETAFLMKVMES 790
           ETAFLMK MES
Sbjct: 781 ETAFLMKAMES 791

BLAST of MC08g2367 vs. NCBI nr
Match: XP_008456457.1 (PREDICTED: protease 2 [Cucumis melo])

HSP 1 Score: 1463 bits (3788), Expect = 0.0
Identity = 703/791 (88.87%), Postives = 748/791 (94.56%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MN+ R ALRHR  ++H ALRRCLHYK PK P PPSP APPKPPKKPQSFTMH +TWEDPY
Sbjct: 1   MNRLRAALRHRRTNIHFALRRCLHYKVPKTPAPPSPPAPPKPPKKPQSFTMHGITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVM GTERLQSKLQSEMASRL F+LSTPP+RWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMGGTERLQSKLQSEMASRLAFELSTPPLRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEE KQYPVLCRRLASLH+EFISN+SPSAGFDYVSG+KIEQKL+DYNQEAERF
Sbjct: 121 PWLYYRRVEEEKQYPVLCRRLASLHDEFISNKSPSAGFDYVSGQKIEQKLIDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRF+AYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFIAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           Q+LLYVVTDQNKRPCRLYCSMIGS D+D LLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QSLLYVVTDQNKRPCRLYCSMIGSIDEDTLLLEEQDDDVHVYIRHTKDFRFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAADPLSGM LIWEC  L HCI+EHHLGDLYLFTDA KGHE VDSHYLLRSPL
Sbjct: 301 SSKVFLIDAADPLSGMELIWECEKLTHCIVEHHLGDLYLFTDASKGHEPVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
           KVDST+RTWEHVF++D DLV++DVDFSHTHLVLILREG+KFRLCAVRLPLPVG KGPI+L
Sbjct: 361 KVDSTSRTWEHVFVDDPDLVIVDVDFSHTHLVLILREGRKFRLCAVRLPLPVGRKGPINL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELELQ+LPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELELQYLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTT + G S +ISN LE+S+GE N +D+QMWNSLSE+YACE +NV S+DG
Sbjct: 481 ILHERTRILYGTTFSAGGSREISNALENSMGEANFDDEQMWNSLSEYYACEHYNVSSDDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VLVPLTV+YSYKCK+EN+NPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLVPLTVIYSYKCKKENENPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWH DGRR KKFNS+QDYISCAKFLAER I+NEEKLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHQDGRRIKKFNSVQDYISCAKFLAERKIVNEEKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA 720
           ELFRAA+LKVPFLDPISTL +PIIPLT ADYEEFGYPGN DDFHAIRRYSPYDNIQKDVA
Sbjct: 661 ELFRAAVLKVPFLDPISTLRNPIIPLTPADYEEFGYPGNEDDFHAIRRYSPYDNIQKDVA 720

Query: 721 YPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESAL 780
           YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILNLT DIVEENRYLHCKESAL
Sbjct: 721 YPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNLTIDIVEENRYLHCKESAL 780

Query: 781 ETAFLMKVMES 790
           ETAFLMK MES
Sbjct: 781 ETAFLMKAMES 791

BLAST of MC08g2367 vs. NCBI nr
Match: XP_004139255.1 (uncharacterized protein LOC101207722 [Cucumis sativus] >KGN60766.1 hypothetical protein Csa_019455 [Cucumis sativus])

HSP 1 Score: 1461 bits (3782), Expect = 0.0
Identity = 705/791 (89.13%), Postives = 743/791 (93.93%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MN+ R  LRHR  HLHG   RCLHYK PK PQPP+P APPKPPKKPQSFT+HE+TWEDPY
Sbjct: 1   MNRLRAVLRHRRTHLHGDFGRCLHYKVPKTPQPPAPPAPPKPPKKPQSFTLHEITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVM GTERLQSKLQSEMASRL F+LSTPP+RWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMGGTERLQSKLQSEMASRLAFELSTPPLRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQYPVLCRRLASLHEEFISN+SPSAGFDYVSG+KIEQKL+DYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNKSPSAGFDYVSGQKIEQKLIDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           Q+LLYVVTDQNKRPCRLYCS IGS D+D LLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QSLLYVVTDQNKRPCRLYCSTIGSIDEDTLLLEEKDDDVHVYIRHTKDFRFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAADPLSGM LIWEC  LAHCI+EHHLGDLYLFTDA KGHE VDSHYLLRSPL
Sbjct: 301 SSKVFLIDAADPLSGMKLIWECEELAHCIVEHHLGDLYLFTDASKGHERVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
           KVDST RTWEHVF++D D V++DVDF HTHLVLILREG+KF LCAVRLPLPVG KGPI L
Sbjct: 361 KVDSTLRTWEHVFVDDPDFVIVDVDFCHTHLVLILREGRKFSLCAVRLPLPVGGKGPISL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELELQ+LPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELELQYLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTTS+ G S +ISN LE+SVGE N  D+QMWNSLSE+YACE +NV S+DG
Sbjct: 481 ILHERTRILYGTTSSAGGSREISNALENSVGEANF-DEQMWNSLSEYYACEHYNVSSDDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VLVPLTVVYSYKCK+EN+NPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLVPLTVVYSYKCKKENENPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWH DGRR KKFNS+QDYISCAKFLAER I+NE+KLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHQDGRRIKKFNSVQDYISCAKFLAERQIVNEDKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA 720
           ELFRAAILKVPFLDPISTLL+PIIPLT ADYEEFGYPGN DDFHAIRRYSPYDNIQKD A
Sbjct: 661 ELFRAAILKVPFLDPISTLLNPIIPLTPADYEEFGYPGNEDDFHAIRRYSPYDNIQKDAA 720

Query: 721 YPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESAL 780
           YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILNLT DIVEENRYLHCKESAL
Sbjct: 721 YPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNLTIDIVEENRYLHCKESAL 780

Query: 781 ETAFLMKVMES 790
           ETAFLMK MES
Sbjct: 781 ETAFLMKAMES 790

BLAST of MC08g2367 vs. NCBI nr
Match: KAG6586337.1 (Protease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1442 bits (3733), Expect = 0.0
Identity = 701/792 (88.51%), Postives = 745/792 (94.07%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MNQFR ALRH  ++LHGALRRCLHYK PK PQPPSP  PPKPPKKPQSFTMH++TWEDPY
Sbjct: 1   MNQFRAALRHCRSNLHGALRRCLHYKTPKTPQPPSPPGPPKPPKKPQSFTMHDITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMS LNDKVAMRHMDVYMEQEEKY EAVMA TERLQSKLQSEMASRL FDLSTP +R G
Sbjct: 61  SWMSRLNDKVAMRHMDVYMEQEEKYVEAVMADTERLQSKLQSEMASRLAFDLSTPSLRLG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQY VLCRRLASLHE+FISN+SPSAGFDYVSGKKIEQKLLDYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYQVLCRRLASLHEKFISNKSPSAGFDYVSGKKIEQKLLDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           QALLYVVTDQNKRPCRLYCSMIGSTD+D LLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QALLYVVTDQNKRPCRLYCSMIGSTDEDTLLLEEPDDDVHVYIRHTKDFSFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAA+PLSGM LIWEC GLAHCIMEHHLG LYLFTDA+KGHEAVDSHYLLRSPL
Sbjct: 301 SSKVFLIDAANPLSGMELIWECEGLAHCIMEHHLGVLYLFTDANKGHEAVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
            V+ST+RTWE+VF++D DLV++DVDFSHTHLVLILREG K RLCAVRLPLPVG KG I+L
Sbjct: 361 SVESTSRTWENVFVDDPDLVIVDVDFSHTHLVLILREGPKLRLCAVRLPLPVGGKGSINL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELE  FLPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELEPHFLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTTS+  ASG+ISN  E S+GE N +DDQMWN+LSEFYACERFNV S+DG
Sbjct: 481 ILHERTRILYGTTSSAAASGEISNESEISMGEANSDDDQMWNTLSEFYACERFNVSSHDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VL+PLTVVYSYK KREN+NPGLLHVHGAYGE LDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLIPLTVVYSYKSKRENENPGLLHVHGAYGEPLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWHHDGRR KKFNS+QDYISCAKFL ER I+NEEKLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHHDGRRTKKFNSVQDYISCAKFLVERKIVNEEKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPG-NVDDFHAIRRYSPYDNIQKDV 720
           ELFR+AILKVPFLDPI+TLLHPIIPLT ADYEEFGYP  ++DDFHA+RRYSPYDNIQKDV
Sbjct: 661 ELFRSAILKVPFLDPINTLLHPIIPLTPADYEEFGYPEEDIDDFHAVRRYSPYDNIQKDV 720

Query: 721 AYPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESA 780
           +YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILN+TTDIVEENRYLHCKESA
Sbjct: 721 SYPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNITTDIVEENRYLHCKESA 780

Query: 781 LETAFLMKVMES 790
           LETAFL+K + S
Sbjct: 781 LETAFLLKFIGS 792

BLAST of MC08g2367 vs. ExPASy TrEMBL
Match: A0A6J1CPD8 (Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111012951 PE=3 SV=1)

HSP 1 Score: 1628 bits (4215), Expect = 0.0
Identity = 790/790 (100.00%), Postives = 790/790 (100.00%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY
Sbjct: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT
Sbjct: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL
Sbjct: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK 420
           KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK
Sbjct: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK 420

Query: 421 ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI 480
           ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI
Sbjct: 421 ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI 480

Query: 481 LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV 540
           LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV
Sbjct: 481 LHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGV 540

Query: 541 LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG 600
           LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG
Sbjct: 541 LVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGG 600

Query: 601 GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE 660
           GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE
Sbjct: 601 GGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCPE 660

Query: 661 LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY 720
           LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY
Sbjct: 661 LFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAY 720

Query: 721 PAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALE 780
           PAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALE
Sbjct: 721 PAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESALE 780

Query: 781 TAFLMKVMES 790
           TAFLMKVMES
Sbjct: 781 TAFLMKVMES 790

BLAST of MC08g2367 vs. ExPASy TrEMBL
Match: A0A1S3C3D5 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103496398 PE=3 SV=1)

HSP 1 Score: 1463 bits (3788), Expect = 0.0
Identity = 703/791 (88.87%), Postives = 748/791 (94.56%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MN+ R ALRHR  ++H ALRRCLHYK PK P PPSP APPKPPKKPQSFTMH +TWEDPY
Sbjct: 1   MNRLRAALRHRRTNIHFALRRCLHYKVPKTPAPPSPPAPPKPPKKPQSFTMHGITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVM GTERLQSKLQSEMASRL F+LSTPP+RWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMGGTERLQSKLQSEMASRLAFELSTPPLRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEE KQYPVLCRRLASLH+EFISN+SPSAGFDYVSG+KIEQKL+DYNQEAERF
Sbjct: 121 PWLYYRRVEEEKQYPVLCRRLASLHDEFISNKSPSAGFDYVSGQKIEQKLIDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRF+AYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFIAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           Q+LLYVVTDQNKRPCRLYCSMIGS D+D LLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QSLLYVVTDQNKRPCRLYCSMIGSIDEDTLLLEEQDDDVHVYIRHTKDFRFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAADPLSGM LIWEC  L HCI+EHHLGDLYLFTDA KGHE VDSHYLLRSPL
Sbjct: 301 SSKVFLIDAADPLSGMELIWECEKLTHCIVEHHLGDLYLFTDASKGHEPVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
           KVDST+RTWEHVF++D DLV++DVDFSHTHLVLILREG+KFRLCAVRLPLPVG KGPI+L
Sbjct: 361 KVDSTSRTWEHVFVDDPDLVIVDVDFSHTHLVLILREGRKFRLCAVRLPLPVGRKGPINL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELELQ+LPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELELQYLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTT + G S +ISN LE+S+GE N +D+QMWNSLSE+YACE +NV S+DG
Sbjct: 481 ILHERTRILYGTTFSAGGSREISNALENSMGEANFDDEQMWNSLSEYYACEHYNVSSDDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VLVPLTV+YSYKCK+EN+NPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLVPLTVIYSYKCKKENENPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWH DGRR KKFNS+QDYISCAKFLAER I+NEEKLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHQDGRRIKKFNSVQDYISCAKFLAERKIVNEEKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA 720
           ELFRAA+LKVPFLDPISTL +PIIPLT ADYEEFGYPGN DDFHAIRRYSPYDNIQKDVA
Sbjct: 661 ELFRAAVLKVPFLDPISTLRNPIIPLTPADYEEFGYPGNEDDFHAIRRYSPYDNIQKDVA 720

Query: 721 YPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESAL 780
           YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILNLT DIVEENRYLHCKESAL
Sbjct: 721 YPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNLTIDIVEENRYLHCKESAL 780

Query: 781 ETAFLMKVMES 790
           ETAFLMK MES
Sbjct: 781 ETAFLMKAMES 791

BLAST of MC08g2367 vs. ExPASy TrEMBL
Match: A0A0A0LL77 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_2G009530 PE=3 SV=1)

HSP 1 Score: 1461 bits (3782), Expect = 0.0
Identity = 705/791 (89.13%), Postives = 743/791 (93.93%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MN+ R  LRHR  HLHG   RCLHYK PK PQPP+P APPKPPKKPQSFT+HE+TWEDPY
Sbjct: 1   MNRLRAVLRHRRTHLHGDFGRCLHYKVPKTPQPPAPPAPPKPPKKPQSFTLHEITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKYTEAVM GTERLQSKLQSEMASRL F+LSTPP+RWG
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMGGTERLQSKLQSEMASRLAFELSTPPLRWG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQYPVLCRRLASLHEEFISN+SPSAGFDYVSG+KIEQKL+DYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNKSPSAGFDYVSGQKIEQKLIDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           Q+LLYVVTDQNKRPCRLYCS IGS D+D LLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QSLLYVVTDQNKRPCRLYCSTIGSIDEDTLLLEEKDDDVHVYIRHTKDFRFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAADPLSGM LIWEC  LAHCI+EHHLGDLYLFTDA KGHE VDSHYLLRSPL
Sbjct: 301 SSKVFLIDAADPLSGMKLIWECEELAHCIVEHHLGDLYLFTDASKGHERVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
           KVDST RTWEHVF++D D V++DVDF HTHLVLILREG+KF LCAVRLPLPVG KGPI L
Sbjct: 361 KVDSTLRTWEHVFVDDPDFVIVDVDFCHTHLVLILREGRKFSLCAVRLPLPVGGKGPISL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELELQ+LPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELELQYLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTTS+ G S +ISN LE+SVGE N  D+QMWNSLSE+YACE +NV S+DG
Sbjct: 481 ILHERTRILYGTTSSAGGSREISNALENSVGEANF-DEQMWNSLSEYYACEHYNVSSDDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VLVPLTVVYSYKCK+EN+NPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLVPLTVVYSYKCKKENENPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWH DGRR KKFNS+QDYISCAKFLAER I+NE+KLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHQDGRRIKKFNSVQDYISCAKFLAERQIVNEDKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVA 720
           ELFRAAILKVPFLDPISTLL+PIIPLT ADYEEFGYPGN DDFHAIRRYSPYDNIQKD A
Sbjct: 661 ELFRAAILKVPFLDPISTLLNPIIPLTPADYEEFGYPGNEDDFHAIRRYSPYDNIQKDAA 720

Query: 721 YPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESAL 780
           YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILNLT DIVEENRYLHCKESAL
Sbjct: 721 YPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNLTIDIVEENRYLHCKESAL 780

Query: 781 ETAFLMKVMES 790
           ETAFLMK MES
Sbjct: 781 ETAFLMKAMES 790

BLAST of MC08g2367 vs. ExPASy TrEMBL
Match: A0A6J1HM32 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111465437 PE=3 SV=1)

HSP 1 Score: 1442 bits (3732), Expect = 0.0
Identity = 699/792 (88.26%), Postives = 745/792 (94.07%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           M+QFR ALRH  ++LHGALRRCLHYKAPK PQPPSP  PPKPPKKPQSFTMH++TWEDPY
Sbjct: 1   MSQFRAALRHCRSNLHGALRRCLHYKAPKTPQPPSPPGPPKPPKKPQSFTMHDITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMSSLNDKVAMRHMDVYMEQEEKY EAVMA TERLQSKLQSEMASRLDFDLSTPP+R G
Sbjct: 61  SWMSSLNDKVAMRHMDVYMEQEEKYVEAVMADTERLQSKLQSEMASRLDFDLSTPPLRLG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQY VLCRRLASLHEEFISN+SPSAGFDYVSGKKIEQKLLDYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYQVLCRRLASLHEEFISNKSPSAGFDYVSGKKIEQKLLDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           QAL YVVTDQNKRPCRLYCSMIGSTD+D LLLEE DDD+HVYIRHTKDF+FVTVNRF+PT
Sbjct: 241 QALFYVVTDQNKRPCRLYCSMIGSTDEDTLLLEEPDDDVHVYIRHTKDFNFVTVNRFTPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDA+DPLS M LIWEC GLAHCIMEHHLG LYLFTDA+KGHEAVDSHYLLRSPL
Sbjct: 301 SSKVFLIDASDPLSRMELIWECEGLAHCIMEHHLGVLYLFTDANKGHEAVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
            V+ST+RTWE+VF++D +LV+LDVDFSHTHLVLILREGQK RLCAVRLPLPVG KG I+L
Sbjct: 361 SVESTSRTWENVFVDDPNLVILDVDFSHTHLVLILREGQKLRLCAVRLPLPVGGKGSINL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELE  FLPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELEPHFLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTTS+  AS KISN  E S GE N +DDQMWN+LSEFYACERFNV S+DG
Sbjct: 481 ILHERTRILYGTTSSAAASAKISNESEISTGEANFDDDQMWNTLSEFYACERFNVSSHDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VL+PLTVVYSYK KREN+NPGLLHVHGAYGE LDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLIPLTVVYSYKSKRENENPGLLHVHGAYGEPLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWHHDGRR KKFNS+QDYISCAKFL ER I++EEKLAGWGYSAGG +VASAINQCP
Sbjct: 601 GGGKKWHHDGRRTKKFNSVQDYISCAKFLVERKIVHEEKLAGWGYSAGGFMVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPG-NVDDFHAIRRYSPYDNIQKDV 720
           ELFR+AILKVPFLDP++TLLHPIIPLT ADYEEFGYP  ++DDFHA+ RYSPYDNIQKDV
Sbjct: 661 ELFRSAILKVPFLDPVNTLLHPIIPLTPADYEEFGYPEEDIDDFHAVCRYSPYDNIQKDV 720

Query: 721 AYPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESA 780
           AYPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILN+TTDIVEENRYLHCKESA
Sbjct: 721 AYPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNITTDIVEENRYLHCKESA 780

Query: 781 LETAFLMKVMES 790
           LETAFL+K + S
Sbjct: 781 LETAFLLKFIGS 792

BLAST of MC08g2367 vs. ExPASy TrEMBL
Match: A0A6J1FJB8 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111444419 PE=3 SV=1)

HSP 1 Score: 1439 bits (3725), Expect = 0.0
Identity = 700/792 (88.38%), Postives = 744/792 (93.94%), Query Frame = 0

Query: 1   MNQFRVALRHRCNHLHGALRRCLHYKAPKPPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 60
           MNQFR ALRH  ++LHGALRRCLHYK PK PQ PSP  PPKPPKKPQSFTMH++TWEDPY
Sbjct: 1   MNQFRAALRHCRSNLHGALRRCLHYKTPKTPQSPSPPGPPKPPKKPQSFTMHDITWEDPY 60

Query: 61  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 120
           SWMS LNDKVAMRHMDVYMEQEEKY EAVMA TERLQSKLQSEMASRL FDLSTP +R G
Sbjct: 61  SWMSRLNDKVAMRHMDVYMEQEEKYVEAVMADTERLQSKLQSEMASRLAFDLSTPSLRLG 120

Query: 121 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 180
           PWLYYRRVEEGKQY VLCRRLASLHE+FISN+SPSAGFDYVSGKKIEQKLLDYNQEAERF
Sbjct: 121 PWLYYRRVEEGKQYQVLCRRLASLHEKFISNKSPSAGFDYVSGKKIEQKLLDYNQEAERF 180

Query: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 240
           GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNL+SGSLCSKPQVDRVSNLAWAKGG
Sbjct: 181 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLSSGSLCSKPQVDRVSNLAWAKGG 240

Query: 241 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 300
           QALLYVVTDQNKRPCRLYCSMIGSTD+D LLLEE DDD+HVYIRHTKDF FVTVNRFSPT
Sbjct: 241 QALLYVVTDQNKRPCRLYCSMIGSTDEDTLLLEEPDDDVHVYIRHTKDFSFVTVNRFSPT 300

Query: 301 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 360
           SSKVFLIDAA+PLSGM LIWEC GLAHCIMEHHLG LYLFTDA+KGHEAVDSHYLLRSPL
Sbjct: 301 SSKVFLIDAANPLSGMELIWECEGLAHCIMEHHLGVLYLFTDANKGHEAVDSHYLLRSPL 360

Query: 361 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVG-KGPIHL 420
            V+ST+RTWE+VF++D DLV++DVDFSHTHLVLILREG K RLCAVRLPLPVG KG I+L
Sbjct: 361 SVESTSRTWENVFVDDPDLVIVDVDFSHTHLVLILREGPKLRLCAVRLPLPVGGKGSINL 420

Query: 421 KELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQS 480
           KELE  FLPLP++VSQIS GPNYDFYSSTMRFTISSPVMPDAVVDY+LSDGKWNIIQQQS
Sbjct: 421 KELEPHFLPLPKHVSQISSGPNYDFYSSTMRFTISSPVMPDAVVDYNLSDGKWNIIQQQS 480

Query: 481 ILHERTRILYGTTSTTGASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNVPSNDG 540
           ILHERTRILYGTTS+  ASG+ISN  E S+GE N +DDQMWN+LSEFYACERFNV S+DG
Sbjct: 481 ILHERTRILYGTTSSAAASGEISNESEISMGEANSDDDQMWNTLSEFYACERFNVSSHDG 540

Query: 541 VLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYADVRGGG 600
           VL+PLTVVYSYK KREN+NPGLLHVHGAYGE LDKRWRSELKSLLDRGWVIAYADVRGGG
Sbjct: 541 VLIPLTVVYSYKSKRENENPGLLHVHGAYGEPLDKRWRSELKSLLDRGWVIAYADVRGGG 600

Query: 601 GGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASAINQCP 660
           GGGKKWHHDGRR KKFNS+QDYISCAKFL ER I+NEEKLAGWGYSAGGLLVASAINQCP
Sbjct: 601 GGGKKWHHDGRRTKKFNSVQDYISCAKFLVERKIVNEEKLAGWGYSAGGLLVASAINQCP 660

Query: 661 ELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPG-NVDDFHAIRRYSPYDNIQKDV 720
           ELFR+AILKVPFLDPI+TLLHPIIPLT ADYEEFGYP  ++DDFHA+RRYSPYDNIQKDV
Sbjct: 661 ELFRSAILKVPFLDPINTLLHPIIPLTPADYEEFGYPEEDIDDFHAVRRYSPYDNIQKDV 720

Query: 721 AYPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHCKESA 780
           +YPAVLITS+FNTRFGVWEAAKWIARVRD+SIYDPKRPVILN+TTDIVEENRYLHCKESA
Sbjct: 721 SYPAVLITSSFNTRFGVWEAAKWIARVRDYSIYDPKRPVILNITTDIVEENRYLHCKESA 780

Query: 781 LETAFLMKVMES 790
           LETAFL+K + S
Sbjct: 781 LETAFLLKFIGS 792

BLAST of MC08g2367 vs. TAIR 10
Match: AT5G66960.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 563/796 (70.73%), Postives = 655/796 (82.29%), Query Frame = 0

Query: 2   NQFRVALRHRCNHLHGALRRCLHYKAPK-PPQPPSPAAPPKPPKKPQSFTMHEVTWEDPY 61
           N  R   RH C       ++C  YK PK PP PP P A PKPPKKPQSFT H+ TWEDPY
Sbjct: 4   NLSRTFSRHNCRFRR---QQCRCYKPPKSPPPPPPPPALPKPPKKPQSFTFHDATWEDPY 63

Query: 62  SWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQSKLQSEMASRLDFDLSTPPIRWG 121
           SWMS L DKVAMRHMD+YMEQEEKYTEAV+A T+R+Q+KLQSEMASRL F+LSTPP+RWG
Sbjct: 64  SWMSKLEDKVAMRHMDIYMEQEEKYTEAVLADTDRIQTKLQSEMASRLSFELSTPPLRWG 123

Query: 122 PWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAGFDYVSGKKIEQKLLDYNQEAERF 181
           PWLYYRRVEEGKQYPVLCRRLASLHEEFIS++SP+AGFDY SGK+IEQKLLDYNQEAERF
Sbjct: 124 PWLYYRRVEEGKQYPVLCRRLASLHEEFISHKSPAAGFDYTSGKRIEQKLLDYNQEAERF 183

Query: 182 GGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNLNSGSLCSKPQVDRVSNLAWAKGG 241
           GGYAYEE+SE+SPDH+FLAYTMYDKDNDYF+L V+NLNSG+LCSKP  DRVSN+AWAK G
Sbjct: 184 GGYAYEEMSEISPDHKFLAYTMYDKDNDYFKLCVRNLNSGALCSKPHADRVSNIAWAKNG 243

Query: 242 QALLYVVTDQNKRPCRLYCSMIGSTDDDILLLEELDDDIHVYIRHTKDFHFVTVNRFSPT 301
           QALLYVVTDQ KRPCR+YCS IGSTD+D+LL EE + ++HV IRHTKDFHFVTVN FS T
Sbjct: 244 QALLYVVTDQKKRPCRIYCSTIGSTDEDVLLHEEFEGNVHVNIRHTKDFHFVTVNTFSTT 303

Query: 302 SSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGDLYLFTDADKGHEAVDSHYLLRSPL 361
            SKVFLI+AADP SG+ L+WE    AHCI+EHH G LYLFT+A      VD HYLLRSP+
Sbjct: 304 FSKVFLINAADPFSGLALVWEHNAPAHCIIEHHQGFLYLFTNASNDGGTVDHHYLLRSPV 363

Query: 362 KVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLILREGQKFRLCAVRLPLPVGKGPIHLK 421
              S  R WE VFI D +L++ DVDF   HL LI++E Q F++C V LPL   + P+HL+
Sbjct: 364 HFSSCQRIWETVFINDPELIIEDVDFCKKHLSLIVKEMQSFKICVVDLPLKTKRVPVHLR 423

Query: 422 ELELQFLPLPENVSQISPGPNYDFYSSTMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSI 481
           +++ ++LPLP++VSQI PG NYDF S TMRFTISS VMPDAVVDY L +GKWNI+QQQ++
Sbjct: 424 DIKPRYLPLPKHVSQIFPGTNYDFNSPTMRFTISSLVMPDAVVDYDLLNGKWNIVQQQNM 483

Query: 482 LHERTRILYGTTSTT------GASGKISNVLEDSVGEGNLNDDQMWNSLSEFYACERFNV 541
           LHERTR+LYGT ++T        +  +S   ED+  E    +D +WN L+EFYAC+   V
Sbjct: 484 LHERTRVLYGTANSTESPNIPSGTRTVSFDTEDTTAE----NDNLWNDLTEFYACDYHEV 543

Query: 542 PSNDGVLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKRWRSELKSLLDRGWVIAYAD 601
            S+DG +VPL++VYS   K EN  PGLLHVHGAYGE+LDKRWRSELKSLLDRGWV+AYAD
Sbjct: 544 SSHDGAMVPLSIVYSRAQKEENQKPGLLHVHGAYGEMLDKRWRSELKSLLDRGWVLAYAD 603

Query: 602 VRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNIINEEKLAGWGYSAGGLLVASA 661
           VRGGGG GKKWH DGR AKK NSI+DYI CAK+L E NI+ E KLAGWGYSAGGL+VASA
Sbjct: 604 VRGGGGKGKKWHQDGRGAKKLNSIKDYIQCAKYLVENNIVEENKLAGWGYSAGGLVVASA 663

Query: 662 INQCPELFRAAILKVPFLDPISTLLHPIIPLTAADYEEFGYPGNVDDFHAIRRYSPYDNI 721
           IN CP+LF+AA+LKVPFLDP  TL++PI+PLTA DYEEFGYPG+++DFHAIR YSPYDNI
Sbjct: 664 INHCPDLFQAAVLKVPFLDPTHTLIYPILPLTAEDYEEFGYPGDINDFHAIREYSPYDNI 723

Query: 722 QKDVAYPAVLITSTFNTRFGVWEAAKWIARVRDFSIYDPKRPVILNLTTDIVEENRYLHC 781
            KDV YPAVL+TS+FNTRFGVWEAAKW+ARVRD +  DP+RPV+LNLTTDIVEENR+L  
Sbjct: 724 PKDVLYPAVLVTSSFNTRFGVWEAAKWVARVRDNTFNDPERPVLLNLTTDIVEENRFLQT 783

Query: 782 KESALETAFLMKVMES 791
           KESALE AFL+K+MES
Sbjct: 784 KESALEIAFLIKMMES 792

BLAST of MC08g2367 vs. TAIR 10
Match: AT1G69020.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 535.4 bits (1378), Expect = 7.6e-152
Identity = 303/763 (39.71%), Postives = 448/763 (58.72%), Query Frame = 0

Query: 38  APPKPPKKPQSFTMHEVTWEDPYSWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQ 97
           APP P K P + + H +T +DP+ WM + +D   +     ++++E  Y++A MA TE L+
Sbjct: 39  APPVPKKIPFAISSHGITRQDPFHWMKNTDDTDFVD----FLKRENSYSQAFMADTETLR 98

Query: 98  SKLQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAG 157
             L SEM +R+  ++ TPP RWG WLY + + +GK+YP+LCRRL      ++S       
Sbjct: 99  RDLFSEMKTRIPEEIFTPPERWGQWLYRQYIPKGKEYPLLCRRLEKGKTNWLSG------ 158

Query: 158 FDYVSGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSVKNL 217
                G++ E+ +LD+NQ AE+F GY +  +  VSPDH +LAYT+ D + D         
Sbjct: 159 --LFRGEE-EEVVLDWNQIAEQF-GYVHVGVCRVSPDHNYLAYTV-DPEGD--------- 218

Query: 218 NSGSLCSKPQVDRVSNLAWAKGGQALLYVVTDQNKRPCRLYCSMIGSTD-DDILLLEELD 277
                                 G  L Y VTD+N+RP R+  + + S   DD ++  E D
Sbjct: 219 ----------------------GITLFYTVTDENQRPHRVVVTNVESDGRDDAVVFTERD 278

Query: 278 DDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSGMVLIWECGGLAHCIMEHHLGD 337
               V I  TKD  FVT+N  S TSS+V++++A  P++G+    E      C +EHH G 
Sbjct: 279 SSFCVDITTTKDGKFVTINSNSRTSSEVYIVNADKPMAGLQRTRERVPGVQCFLEHHNGF 338

Query: 338 LYLFTDADKG--HEAVDSHYLLRSPLKVDSTARTWEHVFIEDQDLVMLDVDFSHTHLVLI 397
            Y+ T++      E     Y L   L  +  A  W+ VF  D D+V+ D+D  + +LVL 
Sbjct: 339 FYILTNSPSNAISEWSGEGYYLTRCLVEEIEASDWQTVFRPDDDVVIQDMDMFNDYLVLY 398

Query: 398 LREGQKFRLCAVRLPLPVGKGPIHLKELELQFLPLPENVSQISPGPNYDFYSSTMRFTIS 457
           L +     LC++ +P+       H+ +L   + PLP +   ++PG N+DF SS  R  +S
Sbjct: 399 LNKKGLPMLCSIDMPIKANTK--HMDDLVPWYFPLPVDSCSVAPGSNHDFQSSIYRVVLS 458

Query: 458 SPVMPDAVVDYHLSDGKWNIIQQQSIL---HERTRILYGTTSTTGASGKISNVLEDSVGE 517
           SPV+PD +VDY +S   ++I+QQ+  +    + ++  Y    +T  +G++++    S GE
Sbjct: 459 SPVIPDTIVDYDVSRRLFSIVQQEGGVVDNSDSSKPWYTADRSTENNGQLND--RTSEGE 518

Query: 518 GNLNDDQM--WNSLSEFYACERFNVPSNDGVLVPLTVVYSYKCKRENDNPGLLHVHGAYG 577
               D +M  W  LS+ Y CER  V S+DGV VPLT++YS +  +++++PG+L  +GAYG
Sbjct: 519 DGQLDSRMPKWEDLSDTYVCERQEVSSHDGVEVPLTILYSREAWKKSESPGMLIGYGAYG 578

Query: 578 ELLDKRWRSELKSLLDRGWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLA 637
           E+LDK W +   S+LDRGWVIA+ADVRGGG G   WH  G R+ K NSIQD+I  AK+L 
Sbjct: 579 EVLDKSWCTNRLSMLDRGWVIAFADVRGGGSGEFSWHKSGTRSLKQNSIQDFIYSAKYLV 638

Query: 638 ERNIINEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPLTAAD 697
           E+  ++   LA  GYSAG +L A+A+N  P LF+A ILKVPF+D ++TL  P +PLT  D
Sbjct: 639 EKGYVHRHHLAAVGYSAGAILPAAAMNMHPSLFQAVILKVPFVDVLNTLSDPNLPLTLLD 698

Query: 698 YEEFGYPGNVDDFHAIRRYSPYDNIQKDVAYPAVLITSTF-NTRFGVWEAAKWIARVRDF 757
           +EEFG P N  DF +I  YSPYD I+KDV YP++L+T++F ++R GVWE AKW+A++RD 
Sbjct: 699 HEEFGNPDNQTDFGSILSYSPYDKIRKDVCYPSMLVTTSFHDSRVGVWEGAKWVAKIRDS 751

Query: 758 SIYDPKRPVILNLTTD---IVEENRYLHCKESALETAFLMKVM 789
           + +D  R VIL    +     E  RY  C+E+A + AFL+KVM
Sbjct: 759 TCHDCSRAVILKTNMNGGHFGEGGRYAQCEETAFDYAFLLKVM 751

BLAST of MC08g2367 vs. TAIR 10
Match: AT1G50380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 271.2 bits (692), Expect = 2.7e-72
Identity = 216/766 (28.20%), Postives = 362/766 (47.26%), Query Frame = 0

Query: 38  APPKPPKKPQSFTMHEVTWEDPYSWMSSLNDKVAMRHMDVYMEQEEKYTEAVMAGTERLQ 97
           +PP   K      M      D Y W+   +D      M  Y+ +E  YT+ VM+GT++ +
Sbjct: 6   SPPVAKKVEHVMEMFGDVRVDNYYWLR--DDSRTNPDMLSYLREENHYTDFVMSGTKQFE 65

Query: 98  SKLQSEMASRLDFDLSTPPIRWGPWLYYRRVEEGKQYPVLCRRLASLHEEFISNRSPSAG 157
           ++L +E+  R+  D  + P+R GP+ YY +  +GK+Y   CRRL +       N++  + 
Sbjct: 66  NQLFAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLIT------DNKAEPSV 125

Query: 158 FDYV---SGKKIEQKLLDYNQEAERFGGYAYEELSEVSPDHRFLAYTMYDKDNDYFRLSV 217
           +D +        E  +LD N +A+    Y      + SPDH+ +AY    K ++ + ++V
Sbjct: 126 YDTMPTGPDAPPEHVILDENTKAQEHDYYRIGAF-KASPDHKLVAYAEDTKGDEIYTVNV 185

Query: 218 KNLNSGSLCSKPQVDRVSNLAWAKGGQALLYVVTDQNKRPCRLYCSMIGS-TDDDILLLE 277
            +  +     +      S L WA G  ALLY+  D+  RP +++   +G+    D+ L  
Sbjct: 186 IDSEALKPVGQQLKGLTSYLEWA-GNDALLYITMDEILRPDKVWLHKLGTEQSSDVCLYH 245

Query: 278 ELDDDIHVYIRHTKDFHFVTVNRFSPTSSKVFLIDAADPLSGM-VLIWECGGLAHCIMEH 337
           E DD   + +  ++   ++ V   S T+  VF +D +    G+ VL     G+   +   
Sbjct: 246 EKDDMFSLELHASESHKYLFVASESKTTRFVFSLDVSKTQDGLRVLTPRVDGIDSSV--S 305

Query: 338 HLGDLYLFTDADKGHEAVDSHYLLRSPLKVDSTARTWEHVFIEDQDLVML-DVDFSHTHL 397
           H G+ +      +  E  +S  L+  P  VD T++T   V +  ++ V + ++     HL
Sbjct: 306 HRGNHFFI--QRRSTEFYNSE-LIACP--VDDTSKT--TVLLPHRESVKIQEIQLFRDHL 365

Query: 398 VLILREGQKFRLCAVRLPLPVGKGPIHLKELELQFLPLPENVSQISPGPNYD-----FYS 457
            +  RE    ++   RLP          +   L+ L    NVS + P  + D     F S
Sbjct: 366 AVFERENGLQKITVHRLP---------AEGQPLEGLQGGRNVSFVDPVYSVDSTESEFSS 425

Query: 458 STMRFTISSPVMPDAVVDYHLSDGKWNIIQQQSILHERTRILYGTTSTTGASGKISNVLE 517
             +RF   S   P +V DY +  G                        T    KI  VL 
Sbjct: 426 RVLRFKYCSMKTPPSVYDYDMDSG------------------------TSVVKKIDTVL- 485

Query: 518 DSVGEGNLNDDQMWNSLSEFYACERFNVPSNDGVLVPLTVVYSYK-CKRENDNPGLLHVH 577
                G  +        +  Y  ER  V ++DG  +P+++VY+ K  K +  +P LL+ +
Sbjct: 486 -----GGFD--------ASNYVTERKWVAASDGTQIPMSIVYNKKLAKLDGSDPLLLYGY 545

Query: 578 GAYGELLDKRWRSELKSLLDRGWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCA 637
           G+Y   +D  +++   SLLDRG+    A VRGGG  G++W+ +G+  KK N+  D+I+CA
Sbjct: 546 GSYEISVDPYFKASRLSLLDRGFTFVIAHVRGGGEMGRQWYENGKLLKKKNTFTDFIACA 605

Query: 638 KFLAERNIINEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPL 697
           + L E    ++EKL   G SAGGLL+ + +N  P+LF+  I  VPF+D ++T+L P IPL
Sbjct: 606 ERLIELKYCSKEKLCMEGRSAGGLLMGAVVNMRPDLFKVVIAGVPFVDVLTTMLDPTIPL 665

Query: 698 TAADYEEFGYPGNVDDFHAIRRYSPYDNIQKDVAYPAVLITSTFN-TRFGVWEAAKWIAR 757
           T +++EE+G P   + +  ++ YSP DN+     YP +L+T+  N  R    E  KW+A+
Sbjct: 666 TTSEWEEWGDPRKEEFYFYMKSYSPVDNVTAQ-NYPNMLVTAGLNDPRVMYSEPGKWVAK 704

Query: 758 VRDFSIYDPKRPVILNL-TTDIVEENRYLHCKESALETAFLMKVME 790
           +R+    +        L      +  R+   +E A   AF+MKV++
Sbjct: 726 LREMKTDNNVLLFKCELGAGHFSKSGRFEKLQEDAFTFAFMMKVLD 704

BLAST of MC08g2367 vs. TAIR 10
Match: AT1G76140.2 (Prolyl oligopeptidase family protein )

HSP 1 Score: 109.8 bits (273), Expect = 1.0e-23
Identity = 73/241 (30.29%), Postives = 119/241 (49.38%), Query Frame = 0

Query: 516 DDQMWNSLSEFYACERFNVPSNDGVLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKR 575
           D + + ++  FY       PS DG  +P+ +V     K +  +P LL+ +G +   +   
Sbjct: 509 DREAFQAIQVFY-------PSKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPS 568

Query: 576 WRSELKSLLDR--GWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNI 635
           + S  + +L +  G V  +A++RGGG  G++WH  G  AKK N   D+IS A++L     
Sbjct: 569 F-SASRIVLSKHLGVVFCFANIRGGGEYGEEWHKAGSLAKKQNCFDDFISGAEYLVSAGY 628

Query: 636 INEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPLTAADYEEF 695
               KL   G S GGLLV + INQ P+L+  A+  V  +D +      I     +DY   
Sbjct: 629 TQPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDY--- 688

Query: 696 GYPGNVDDFHAIRRYSPYDNIQKD--------VAYPA-VLITSTFNTRFGVWEAAKWIAR 746
           G   N ++FH + +YSP  N+++         V YP+ +L+T+  + R     + K +A 
Sbjct: 689 GCSENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLAH 738

BLAST of MC08g2367 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 108.6 bits (270), Expect = 2.3e-23
Identity = 72/242 (29.75%), Postives = 120/242 (49.59%), Query Frame = 0

Query: 516 DDQMWNSLSEFYACERFNVPSNDGVLVPLTVVYSYKCKRENDNPGLLHVHGAYGELLDKR 575
           D + + ++  FY       PS DG  +P+ +V     K +  +P LL+ +G +   +   
Sbjct: 509 DREAFQAIQVFY-------PSKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPS 568

Query: 576 WRSELKSLLDR--GWVIAYADVRGGGGGGKKWHHDGRRAKKFNSIQDYISCAKFLAERNI 635
           + S  + +L +  G V  +A++RGGG  G++WH  G  AKK N   D+IS A++L     
Sbjct: 569 F-SASRIVLSKHLGVVFCFANIRGGGEYGEEWHKAGSLAKKQNCFDDFISGAEYLVSAGY 628

Query: 636 INEEKLAGWGYSAGGLLVASAINQCPELFRAAILKVPFLDPISTLLHPIIPLTAADYEEF 695
               KL   G S GGLLV + INQ P+L+  A+  V  +D +      I     +DY   
Sbjct: 629 TQPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDY--- 688

Query: 696 GYPGNVDDFHAIRRYSPYDNIQKD--------VAYPA-VLITSTFNTRFGVWEAAKWIAR 747
           G   N ++FH + +YSP  N+++         V YP+ +L+T+  + R     + K +A 
Sbjct: 689 GCSENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLAT 739

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q595367.1e-7026.83Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
O078343.5e-6927.54Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
P245555.0e-5525.58Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
Q4J6C61.5e-5126.41Prolyl endopeptidase-like OS=Homo sapiens OX=9606 GN=PREPL PE=1 SV=1[more]
Q8C1671.5e-5129.17Prolyl endopeptidase-like OS=Mus musculus OX=10090 GN=Prepl PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022142957.10.0100.00uncharacterized protein LOC111012951 [Momordica charantia][more]
XP_038889448.10.090.77dipeptidyl aminopeptidase BI [Benincasa hispida][more]
XP_008456457.10.088.87PREDICTED: protease 2 [Cucumis melo][more]
XP_004139255.10.089.13uncharacterized protein LOC101207722 [Cucumis sativus] >KGN60766.1 hypothetical ... [more]
KAG6586337.10.088.51Protease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1CPD80.0100.00Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111012951 PE=3 SV=1[more]
A0A1S3C3D50.088.87Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103496398 PE=3 SV=1[more]
A0A0A0LL770.089.13Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_2G009530 PE=3 SV=1[more]
A0A6J1HM320.088.26Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111465437 PE=3 SV=1[more]
A0A6J1FJB80.088.38Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111444419 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G66960.10.0e+0070.73Prolyl oligopeptidase family protein [more]
AT1G69020.17.6e-15239.71Prolyl oligopeptidase family protein [more]
AT1G50380.12.7e-7228.20Prolyl oligopeptidase family protein [more]
AT1G76140.21.0e-2330.29Prolyl oligopeptidase family protein [more]
AT1G76140.12.3e-2329.75Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002470Peptidase S9A, prolyl oligopeptidasePRINTSPR00862PROLIGOPTASEcoord: 613..632
score: 28.68
coord: 559..577
score: 24.65
coord: 585..609
score: 47.16
coord: 701..716
score: 35.86
coord: 643..663
score: 51.63
coord: 719..741
score: 22.43
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 42..477
e-value: 3.0E-42
score: 144.9
NoneNo IPR availableGENE3D2.130.10.120coord: 111..479
e-value: 2.2E-69
score: 235.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..48
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 20..790
NoneNo IPR availablePANTHERPTHR11757:SF6PROLYL OLIGOPEPTIDASE FAMILY PROTEINcoord: 20..790
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 35..480
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 578..746
e-value: 1.2E-38
score: 132.8
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 497..790
e-value: 2.8E-78
score: 265.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 528..748

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2367.1MC08g2367.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity