MC08g2353 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2353
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	VQ domain-containing protein
Location	MC08: 32388418 .. 32388813 (+)
RNA-Seq Expression	MC08g2353
Synteny	MC08g2353

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACCTTAGAAGAAGCCTTCCTGATCATCACCGTCATCACCAAAGAAGCCAAGCTCCCAATAGAGCAATAATGGAGAAGCCCATCAAAGTCAAGTACATTTCGAACCCGATGATGGTGAAGGCGAACTCCGAATCAGAGTTTCGAGAAATAGTTCAGAGGCTTACCGGCCAGAACTCGCCCGATGATGCTTTTGAATCACCAGCTTTTGGAGGAGAAGAAGAATTAAACCATGCTTTTTATCCTCCTCCGCCGTCTACGGCGGTGTCGACGGACCCTAAGGGTTATTATGGCTTGGTCTCCGGTAAGGTCGGCGAGCTCGGCGAAGGCTGCTTCCGGCAAGATGAAATGCAGGGTTTTTCTGGGTTTCAAGCTTCTTGCGTTAATACGTAT

mRNA sequence

ATGGATAACCTTAGAAGAAGCCTTCCTGATCATCACCGTCATCACCAAAGAAGCCAAGCTCCCAATAGAGCAATAATGGAGAAGCCCATCAAAGTCAAGTACATTTCGAACCCGATGATGGTGAAGGCGAACTCCGAATCAGAGTTTCGAGAAATAGTTCAGAGGCTTACCGGCCAGAACTCGCCCGATGATGCTTTTGAATCACCAGCTTTTGGAGGAGAAGAAGAATTAAACCATGCTTTTTATCCTCCTCCGCCGTCTACGGCGGTGTCGACGGACCCTAAGGGTTATTATGGCTTGGTCTCCGGTAAGGTCGGCGAGCTCGGCGAAGGCTGCTTCCGGCAAGATGAAATGCAGGGTTTTTCTGGGTTTCAAGCTTCTTGCGTTAATACGTAT

Coding sequence (CDS)

ATGGATAACCTTAGAAGAAGCCTTCCTGATCATCACCGTCATCACCAAAGAAGCCAAGCTCCCAATAGAGCAATAATGGAGAAGCCCATCAAAGTCAAGTACATTTCGAACCCGATGATGGTGAAGGCGAACTCCGAATCAGAGTTTCGAGAAATAGTTCAGAGGCTTACCGGCCAGAACTCGCCCGATGATGCTTTTGAATCACCAGCTTTTGGAGGAGAAGAAGAATTAAACCATGCTTTTTATCCTCCTCCGCCGTCTACGGCGGTGTCGACGGACCCTAAGGGTTATTATGGCTTGGTCTCCGGTAAGGTCGGCGAGCTCGGCGAAGGCTGCTTCCGGCAAGATGAAATGCAGGGTTTTTCTGGGTTTCAAGCTTCTTGCGTTAATACGTAT

Protein sequence

MDNLRRSLPDHHRHHQRSQAPNRAIMEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPPPSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY

Homology

[Close]

BLAST of MC08g2353 vs. ExPASy Swiss-Prot
Match: Q9LDH1 (Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 8.7e-05
Identity = 40/100 (40.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 28  KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPPPS 87
           KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D   E P +    + +H   PP  +
Sbjct: 38  KPIKVRYISNPMRVQ-TCASKFRELVQELTGQDAVDLQPE-PIYSPSSD-DHNLSPPAEN 97

Query: 88  TAVSTDPKGYYGLVSGKVGE--LGEGCFRQDEMQ-GFSGF 125
            A     +  +G       E    E  F  D+M  GFSGF
Sbjct: 98  LAPRVLHQEPFGERDSDCYEPLNAEDMFLPDQMSAGFSGF 134

[Close]

BLAST of MC08g2353 vs. ExPASy Swiss-Prot
Match: O80669 (Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB2 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.3e-04
Identity = 28/66 (42.42%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 28 KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPPPS 87
          KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D                   PP P+
Sbjct: 35 KPIKVRYISNPMRVE-TCPSKFRELVQELTGQDAAD------------------LPPSPT 81

Query: 88 TAVSTD 94
          T  + D
Sbjct: 95 TFTAVD 81

[Close]

BLAST of MC08g2353 vs. NCBI nr
Match: XP_022142960.1 (sigma factor binding protein 1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 222 bits (565), Expect = 1.39e-72
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = 0


Query: 26  MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPP 85
           MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPP
Sbjct: 1   MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPP 60

Query: 86  PSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 132
           PSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY
Sbjct: 61  PSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 107

[Close]

BLAST of MC08g2353 vs. NCBI nr
Match: KAA0063579.1 (VQ motif-containing protein [Cucumis melo var. makuwa] >TYJ97906.1 VQ motif-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 114 bits (285), Expect = 1.77e-29
Identity = 73/147 (49.66%), Postives = 95/147 (64.63%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAI----MEKPIKVKYISNPMMVKANSESEFREIVQRL 60
           M+NL  +LP    H + SQ  N+        KPIKVKYIS+PMMVKAN+ESEFR IVQ+L
Sbjct: 1   MENLITTLPTDFHHRKVSQNSNKPTTIDQQNKPIKVKYISSPMMVKANNESEFRAIVQKL 60

Query: 61  TGQNSPDDAFESPAFGGEEELNHAFYPPPPSTA---VSTDPKGYYGLVSG------KVGE 120
           TGQ+SPD   ++      ++ NHAFYPPPPS++   VS DP+  YG  SG      ++ +
Sbjct: 61  TGQHSPD--HDANFDQSVQDFNHAFYPPPPSSSTPGVSFDPRDCYGSASGDVLFDDRIED 120

Query: 121 LGEGCFRQDEMQG-FSGFQAS-CVNTY 132
             EG + + EM+G FSGFQAS CVN +
Sbjct: 121 GDEGRYWRGEMEGSFSGFQASSCVNIW 145

[Close]

BLAST of MC08g2353 vs. NCBI nr
Match: KAE8651551.1 (hypothetical protein Csa_019210 [Cucumis sativus])

HSP 1 Score: 110 bits (275), Expect = 6.10e-28
Identity = 69/140 (49.29%), Postives = 93/140 (66.43%), Query Frame = 0


Query: 7   SLPDHHRHHQRSQAPNRAIME---KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPD 66
           +LP +  H + SQ  N+A ++   KPIKVKYIS+PMMVKAN+ESEFR IVQ+LTGQ+SPD
Sbjct: 6   TLPSNFHHKKVSQVSNKATIDHQNKPIKVKYISSPMMVKANNESEFRAIVQKLTGQHSPD 65

Query: 67  DAFESPAFGGEEELNHAFYPPPPSTA--VSTDPKGYYG------LVSGKVGELGEGCFRQ 126
              +S      ++ NH FYPPPPS++  VS DPK  YG      ++  ++ +  EG + +
Sbjct: 66  HDDQS-----VQDFNHVFYPPPPSSSPGVSFDPKDCYGNSASGDVLFDRIEDGDEGRYWR 125

Query: 127 DEMQ--GFSGFQAS-CVNTY 132
            EM+   FSGFQAS CVN +
Sbjct: 126 GEMELGSFSGFQASSCVNIW 140

[Close]

BLAST of MC08g2353 vs. NCBI nr
Match: XP_038877668.1 (sigma factor binding protein 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 106 bits (264), Expect = 8.05e-27
Identity = 67/109 (61.47%), Postives = 75/109 (68.81%), Query Frame = 0


Query: 28  KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPPPS 87
           KPIKVKYIS+PMMVKAN+ESEFR IVQ+LTGQ+SP DAF+  A    E+ N  FYPPP S
Sbjct: 5   KPIKVKYISSPMMVKANNESEFRAIVQKLTGQHSPHDAFDQSA----EDFNQVFYPPPSS 64

Query: 88  TAV-STDPKGYYGLVSGKVGELGEGCFR-QDEMQG-FSGFQAS-CVNTY 132
            A  S DPK  Y   SG V       F  Q EM+G FSGFQAS CVNT+
Sbjct: 65  MAAPSFDPKDCYASASGDV------LFDWQGEMEGSFSGFQASSCVNTW 103

[Close]

BLAST of MC08g2353 vs. NCBI nr
Match: XP_022938305.1 (sigma factor binding protein 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 101 bits (252), Expect = 9.91e-25
Identity = 69/139 (49.64%), Postives = 85/139 (61.15%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAIM-EKPIKVKYISNPMMVKANSESEFREIVQRLTGQ 60
           M+NL+ SLP+H RH +   +  RA M  KPIKVKYIS+PMMVKAN+E EFR IVQ+LTGQ
Sbjct: 1   MENLK-SLPNHLRHEKLEASNYRATMANKPIKVKYISSPMMVKANNEFEFRAIVQKLTGQ 60

Query: 61  NSPDDAFESPAFGGEEELNHAFYPPPPSTAVSTDPKGYYGLVSGK------VGELGEGCF 120
           +S DDAF+       +E NHAF PP  ST  S D K  Y LVS        VGE+ +  +
Sbjct: 61  HSADDAFDR----SPQEFNHAFCPPS-STVTSFDSKDCYDLVSSDAYDPIGVGEMKDSLY 120

Query: 121 RQDEMQGFSGFQASCVNTY 132
                    GFQAS V+ +
Sbjct: 121 ---------GFQASYVDVW 124

[Close]

BLAST of MC08g2353 vs. ExPASy TrEMBL
Match: A0A6J1CME4 (sigma factor binding protein 1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111012954 PE=4 SV=1)

HSP 1 Score: 222 bits (565), Expect = 6.74e-73
Identity = 107/107 (100.00%), Postives = 107/107 (100.00%), Query Frame = 0


Query: 26  MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPP 85
           MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPP
Sbjct: 1   MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPP 60

Query: 86  PSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 132
           PSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY
Sbjct: 61  PSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 107

[Close]

BLAST of MC08g2353 vs. ExPASy TrEMBL
Match: A0A5A7VDH6 (VQ motif-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001220 PE=4 SV=1)

HSP 1 Score: 114 bits (285), Expect = 8.57e-30
Identity = 73/147 (49.66%), Postives = 95/147 (64.63%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAI----MEKPIKVKYISNPMMVKANSESEFREIVQRL 60
           M+NL  +LP    H + SQ  N+        KPIKVKYIS+PMMVKAN+ESEFR IVQ+L
Sbjct: 1   MENLITTLPTDFHHRKVSQNSNKPTTIDQQNKPIKVKYISSPMMVKANNESEFRAIVQKL 60

Query: 61  TGQNSPDDAFESPAFGGEEELNHAFYPPPPSTA---VSTDPKGYYGLVSG------KVGE 120
           TGQ+SPD   ++      ++ NHAFYPPPPS++   VS DP+  YG  SG      ++ +
Sbjct: 61  TGQHSPD--HDANFDQSVQDFNHAFYPPPPSSSTPGVSFDPRDCYGSASGDVLFDDRIED 120

Query: 121 LGEGCFRQDEMQG-FSGFQAS-CVNTY 132
             EG + + EM+G FSGFQAS CVN +
Sbjct: 121 GDEGRYWRGEMEGSFSGFQASSCVNIW 145

[Close]

BLAST of MC08g2353 vs. ExPASy TrEMBL
Match: A0A0A0LFF9 (VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010160 PE=4 SV=1)

HSP 1 Score: 110 bits (275), Expect = 2.42e-28
Identity = 69/140 (49.29%), Postives = 93/140 (66.43%), Query Frame = 0


Query: 7   SLPDHHRHHQRSQAPNRAIME---KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPD 66
           +LP +  H + SQ  N+A ++   KPIKVKYIS+PMMVKAN+ESEFR IVQ+LTGQ+SPD
Sbjct: 6   TLPSNFHHKKVSQVSNKATIDHQNKPIKVKYISSPMMVKANNESEFRAIVQKLTGQHSPD 65

Query: 67  DAFESPAFGGEEELNHAFYPPPPSTA--VSTDPKGYYG------LVSGKVGELGEGCFRQ 126
              +S      ++ NH FYPPPPS++  VS DPK  YG      ++  ++ +  EG + +
Sbjct: 66  HDDQS-----VQDFNHVFYPPPPSSSPGVSFDPKDCYGNSASGDVLFDRIEDGDEGRYWR 125

Query: 127 DEMQ--GFSGFQAS-CVNTY 132
            EM+   FSGFQAS CVN +
Sbjct: 126 GEMELGSFSGFQASSCVNIW 140

[Close]

BLAST of MC08g2353 vs. ExPASy TrEMBL
Match: A0A6J1FJE0 (sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111444440 PE=4 SV=1)

HSP 1 Score: 101 bits (252), Expect = 4.80e-25
Identity = 69/139 (49.64%), Postives = 85/139 (61.15%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAIM-EKPIKVKYISNPMMVKANSESEFREIVQRLTGQ 60
           M+NL+ SLP+H RH +   +  RA M  KPIKVKYIS+PMMVKAN+E EFR IVQ+LTGQ
Sbjct: 1   MENLK-SLPNHLRHEKLEASNYRATMANKPIKVKYISSPMMVKANNEFEFRAIVQKLTGQ 60

Query: 61  NSPDDAFESPAFGGEEELNHAFYPPPPSTAVSTDPKGYYGLVSGK------VGELGEGCF 120
           +S DDAF+       +E NHAF PP  ST  S D K  Y LVS        VGE+ +  +
Sbjct: 61  HSADDAFDR----SPQEFNHAFCPPS-STVTSFDSKDCYDLVSSDAYDPIGVGEMKDSLY 120

Query: 121 RQDEMQGFSGFQASCVNTY 132
                    GFQAS V+ +
Sbjct: 121 ---------GFQASYVDVW 124

[Close]

BLAST of MC08g2353 vs. ExPASy TrEMBL
Match: A0A0D3BXG3 (VQ domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 GN=106339955 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.07e-11
Identity = 48/109 (44.04%), Postives = 61/109 (55.96%), Query Frame = 0


Query: 19  QAPNRAIMEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELN 78
           Q   RA   KPIKV+YISNPM VK  + S+FRE+VQ LTGQ++ D   E P F      +
Sbjct: 26  QMKKRASRNKPIKVRYISNPMRVKTCA-SKFRELVQELTGQDAVDLELE-PEFSPSAVSD 85

Query: 79  HAFYPPPPSTAVSTDP--KGYYGLVSGKVGEL-GEGCFRQDEMQGFSGF 124
           H+  PPPP T     P  + +  LV+G    L GE  F     +GFSG+
Sbjct: 86  HSPTPPPPETFAPHIPHQEPFDDLVAGYYEPLDGEEMFLSQMSRGFSGY 132

[Close]

BLAST of MC08g2353 vs. TAIR 10
Match: AT3G56710.1 (sigma factor binding protein 1 )

HSP 1 Score: 48.1 bits (113), Expect = 6.2e-06
Identity = 40/100 (40.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 28  KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPPPS 87
           KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D   E P +    + +H   PP  +
Sbjct: 38  KPIKVRYISNPMRVQ-TCASKFRELVQELTGQDAVDLQPE-PIYSPSSD-DHNLSPPAEN 97

Query: 88  TAVSTDPKGYYGLVSGKVGE--LGEGCFRQDEMQ-GFSGF 125
            A     +  +G       E    E  F  D+M  GFSGF
Sbjct: 98  LAPRVLHQEPFGERDSDCYEPLNAEDMFLPDQMSAGFSGF 134

[Close]

BLAST of MC08g2353 vs. TAIR 10
Match: AT2G41180.1 (VQ motif-containing protein )

HSP 1 Score: 45.8 bits (107), Expect = 3.1e-05
Identity = 28/66 (42.42%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 28 KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEELNHAFYPPPPS 87
          KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D                   PP P+
Sbjct: 35 KPIKVRYISNPMRVE-TCPSKFRELVQELTGQDAAD------------------LPPSPT 81

Query: 88 TAVSTD 94
          T  + D
Sbjct: 95 TFTAVD 81

The following BLAST results are available for this feature:

BLAST of MC08g2353 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDH1	8.7e-05	40.00	Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O80669	4.3e-04	42.42	Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of MC08g2353 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142960.1	1.39e-72	100.00	sigma factor binding protein 1, chloroplastic-like [Momordica charantia]	[more]
KAA0063579.1	1.77e-29	49.66	VQ motif-containing protein [Cucumis melo var. makuwa] >TYJ97906.1 VQ motif-cont...	[more]
KAE8651551.1	6.10e-28	49.29	hypothetical protein Csa_019210 [Cucumis sativus]	[more]
XP_038877668.1	8.05e-27	61.47	sigma factor binding protein 1, chloroplastic-like [Benincasa hispida]	[more]
XP_022938305.1	9.91e-25	49.64	sigma factor binding protein 1, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of MC08g2353 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CME4	6.74e-73	100.00	sigma factor binding protein 1, chloroplastic-like OS=Momordica charantia OX=367...	[more]
A0A5A7VDH6	8.57e-30	49.66	VQ motif-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A0A0LFF9	2.42e-28	49.29	VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010160 PE=4 SV=...	[more]
A0A6J1FJE0	4.80e-25	49.64	sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662...	[more]
A0A0D3BXG3	2.07e-11	44.04	VQ domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 GN=106...	[more]

BLAST of MC08g2353 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56710.1	6.2e-06	40.00	sigma factor binding protein 1	[more]
AT2G41180.1	3.1e-05	42.42	VQ motif-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008889	VQ	PFAM	PF05678	VQ	coord: 35..63 e-value: 3.0E-9 score: 36.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..93
IPR039335	Sigma factor binding protein 1/2	PANTHER	PTHR33624	SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTIC	coord: 11..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2353.1	MC08g2353.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051091	positive regulation of DNA-binding transcription factor activity