MC08g2278 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2278
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cytochrome P450 71A1-like
Location	MC08: 31564023 .. 31564385 (-)
RNA-Seq Expression	MC08g2278
Synteny	MC08g2278

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGAAGAGAAAGACATGGCTAATTTGACTTACATCAACGCGATTATAAAGGAGATGATGAGGCTGCTTCTTGTAGGACCAATGTTGGTGCCTAGATTGAGTCGAGAGGATTGTCAAATTGCAGGCTATGACATAGCTAAAGGAACCAGAGTGCTTGTAAATGTGTGGACGATTGGGAGAGATCATGTAGTATGGGAAAATTCAAATGAATTTATTCCAGAAAGGTTCTTTGGAAATAACATTGATGTGAAAGGTCAATACTTTGAGCTTCTCCCATTTAGGTCTCAAAGGAGGATGTGCCCTCGGTATAATCTTGGCGTTAAGGTTGTTCAATCAAGTTTGGCCAATTTATTGCATGGATTT

mRNA sequence

GTGGAAGAGAAAGACATGGCTAATTTGACTTACATCAACGCGATTATAAAGGAGATGATGAGGCTGCTTCTTGTAGGACCAATGTTGGTGCCTAGATTGAGTCGAGAGGATTGTCAAATTGCAGGCTATGACATAGCTAAAGGAACCAGAGTGCTTGTAAATGTGTGGACGATTGGGAGAGATCATGTAGTATGGGAAAATTCAAATGAATTTATTCCAGAAAGGTTCTTTGGAAATAACATTGATGTGAAAGGTCAATACTTTGAGCTTCTCCCATTTAGGTCTCAAAGGAGGATGTGCCCTCGGTATAATCTTGGCGTTAAGGTTGTTCAATCAAGTTTGGCCAATTTATTGCATGGATTT

Coding sequence (CDS)

GTGGAAGAGAAAGACATGGCTAATTTGACTTACATCAACGCGATTATAAAGGAGATGATGAGGCTGCTTCTTGTAGGACCAATGTTGGTGCCTAGATTGAGTCGAGAGGATTGTCAAATTGCAGGCTATGACATAGCTAAAGGAACCAGAGTGCTTGTAAATGTGTGGACGATTGGGAGAGATCATGTAGTATGGGAAAATTCAAATGAATTTATTCCAGAAAGGTTCTTTGGAAATAACATTGATGTGAAAGGTCAATACTTTGAGCTTCTCCCATTTAGGTCTCAAAGGAGGATGTGCCCTCGGTATAATCTTGGCGTTAAGGTTGTTCAATCAAGTTTGGCCAATTTATTGCATGGATTT

Protein sequence

VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRDHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHGF

Homology

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BLAST of MC08g2278 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.1e-33
Identity = 64/121 (52.89%), Postives = 88/121 (72.73%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           V+E D+  LTYI+AIIKE +RL   GP+L PR+  EDC IAGY +  GTR++VN W I R
Sbjct: 359 VDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQR 418

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D +VW   +E+ PERF   ++D+KGQ+FEL+PF S RR CP  +L ++V+  +LA++LHG
Sbjct: 419 DPLVWSQPHEYQPERFLERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHG 478

Query: 121 F 122
           F
Sbjct: 479 F 479

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BLAST of MC08g2278 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 4.5e-32
Identity = 63/121 (52.07%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEE D+  L Y+N ++KE  RL  V P+L+P  S EDC + GY I K +RVL+NVW IGR
Sbjct: 336 VEESDLEKLDYLNMVVKETFRLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGR 395

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D   W ++ +FIPERF G+++DV+G +F+L+PF S RR CP   LG+ VVQ  LA L+H 
Sbjct: 396 DPNAWTDAEKFIPERFEGSSVDVRGNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHC 455

Query: 121 F 122
           F
Sbjct: 456 F 456

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BLAST of MC08g2278 vs. ExPASy Swiss-Prot
Match: A0A1D6F9Y9 (Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 8.5e-31
Identity = 65/126 (51.59%), Postives = 89/126 (70.63%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           V E+D+ +L YI+A++KE MRL  VGP+LVP  +RE   +AGYD+  G RVLVNVW I R
Sbjct: 360 VTERDLPDLPYIDAVVKETMRLHPVGPLLVPHHAREHTVVAGYDVPAGARVLVNVWAIAR 419

Query: 61  DHVVWENS-NEFIPERFF----GNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLA 120
           D   W ++ + F PERF     G ++DV+G +FELLPF + RRMCP + L +K+V + +A
Sbjct: 420 DPASWPDAPDAFRPERFLNGSSGASVDVRGAHFELLPFGAGRRMCPAHGLAMKLVTAGVA 479

Query: 121 NLLHGF 122
           NL+HGF
Sbjct: 480 NLVHGF 485

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BLAST of MC08g2278 vs. ExPASy Swiss-Prot
Match: A0A1D6HSP4 (Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.5e-30
Identity = 64/124 (51.61%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           V E+D+  L YI+A++KE +RL  VGP+LVP  +RE   +AGYD+  G RVLVN W I R
Sbjct: 362 VAERDLPELHYIDAVVKETLRLHPVGPLLVPHYARERTVVAGYDVPAGARVLVNAWAIAR 421

Query: 61  DHVVWENS-NEFIPERFFG--NNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANL 120
           D   W ++ + F PERF G    +DV+G +FELLPF S RR+CP Y+L +K+V + +ANL
Sbjct: 422 DPASWPDAPDAFQPERFLGAAAAVDVRGAHFELLPFGSGRRICPAYDLAMKLVAAGVANL 481

Query: 121 LHGF 122
           +HGF
Sbjct: 482 VHGF 485

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BLAST of MC08g2278 vs. ExPASy Swiss-Prot
Match: B4FVP3 (Zealexin A1 synthase OS=Zea mays OX=4577 GN=CYP71Z18 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.2e-30
Identity = 63/120 (52.50%), Postives = 82/120 (68.33%), Query Frame = 0


Query: 2   EEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGRD 61
           ++   ANL Y+  ++KE +RL    P+L+PR  RE CQ+ GYDI KGT V VNVW I RD
Sbjct: 344 DDLSTANLRYLKLVVKEALRLHCPVPLLLPRKCREACQVMGYDIPKGTCVFVNVWAICRD 403

Query: 62  HVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHGF 121
              WE++ EF PERF  +N+D KG Y+E LPF S RRMCP  NLGV  ++ +LA+LL+ F
Sbjct: 404 PRYWEDAEEFKPERFENSNLDYKGTYYEYLPFGSGRRMCPGANLGVANLELALASLLYHF 463

[Close]

BLAST of MC08g2278 vs. NCBI nr
Match: XP_022150738.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 202 bits (514), Expect = 4.54e-60
Identity = 99/121 (81.82%), Postives = 106/121 (87.60%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+ANL YINAI+KE MRL  + PMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR
Sbjct: 347 VEEKDIANLPYINAIVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 406

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  +WEN N+FIPERF G +IDVKGQ FELLP  S RRMCP YNLG+KVVQSSLANLLHG
Sbjct: 407 DPAIWENPNDFIPERFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHG 466

[Close]

BLAST of MC08g2278 vs. NCBI nr
Match: KAG7018217.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 194 bits (494), Expect = 4.32e-57
Identity = 96/121 (79.34%), Postives = 104/121 (85.95%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+A+L YI+AI KE MRL  V PMLVPR+ REDCQIAGYDIAKGTRVLVNVWTIGR
Sbjct: 349 VEEKDIASLPYIDAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGR 408

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN NEF PERF G +IDVKGQ FELLPF S RRMCP Y+LG+KV+QSSLANLLHG
Sbjct: 409 DPAVWENPNEFSPERFMGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHG 468

[Close]

BLAST of MC08g2278 vs. NCBI nr
Match: XP_022956310.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 194 bits (494), Expect = 4.32e-57
Identity = 96/121 (79.34%), Postives = 104/121 (85.95%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+A+L YI+AI KE MRL  V PMLVPR+ REDCQIAGYDIAKGTRVLVNVWTIGR
Sbjct: 349 VEEKDIASLPYIDAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGR 408

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN NEF PERF G +IDVKGQ FELLPF S RRMCP Y+LG+KV+QSSLANLLHG
Sbjct: 409 DPAVWENPNEFSPERFMGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHG 468

[Close]

BLAST of MC08g2278 vs. NCBI nr
Match: XP_038876425.1 (trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 193 bits (490), Expect = 3.17e-56
Identity = 96/121 (79.34%), Postives = 102/121 (84.30%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+ NL YI+AI KE MRL  V PMLVPR+SREDCQIAGYDIAKGTRVLVNVWTIGR
Sbjct: 382 VEEKDVVNLPYIDAIAKETMRLHPVAPMLVPRMSREDCQIAGYDIAKGTRVLVNVWTIGR 441

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN  EF PERF G  IDVKGQ FELLPF S RRMCP Y+LG+KV+QSSLANLLHG
Sbjct: 442 DPTVWENPYEFNPERFMGKTIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHG 501

[Close]

BLAST of MC08g2278 vs. NCBI nr
Match: XP_023526994.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 192 bits (487), Expect = 4.68e-56
Identity = 94/121 (77.69%), Postives = 103/121 (85.12%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+A+L Y++AI KE MRL  V PMLVPR+ REDC+IAGYDIAKGTRVLVNVWTIGR
Sbjct: 349 VEEKDIASLPYMDAIAKETMRLHPVAPMLVPRMCREDCEIAGYDIAKGTRVLVNVWTIGR 408

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN NEF PERF G  IDVKGQ FELLPF S RRMCP Y+LG+KV+QSSLANLLHG
Sbjct: 409 DPAVWENPNEFSPERFMGKTIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHG 468

[Close]

BLAST of MC08g2278 vs. ExPASy TrEMBL
Match: A0A6J1DA87 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111018796 PE=3 SV=1)

HSP 1 Score: 202 bits (514), Expect = 2.20e-60
Identity = 99/121 (81.82%), Postives = 106/121 (87.60%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+ANL YINAI+KE MRL  + PMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR
Sbjct: 347 VEEKDIANLPYINAIVKETMRLHPIAPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 406

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  +WEN N+FIPERF G +IDVKGQ FELLP  S RRMCP YNLG+KVVQSSLANLLHG
Sbjct: 407 DPAIWENPNDFIPERFSGKSIDVKGQDFELLPLGSGRRMCPGYNLGLKVVQSSLANLLHG 466

[Close]

BLAST of MC08g2278 vs. ExPASy TrEMBL
Match: A0A0A0LCL6 (Flavonoid 3-hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_3G643770 PE=3 SV=1)

HSP 1 Score: 190 bits (482), Expect = 7.96e-58
Identity = 95/121 (78.51%), Postives = 102/121 (84.30%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+ NL YI+AI KE MRL  V PMLVPR++RED QIAGYDIAKGTRVLVNVWTIGR
Sbjct: 138 VEEKDIVNLPYIDAIAKETMRLHPVAPMLVPRMAREDSQIAGYDIAKGTRVLVNVWTIGR 197

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWE+  EF PERF G NIDVKGQ FELLPF S RRMCP YNLG+KV+QSSLANLLHG
Sbjct: 198 DPTVWEDPLEFKPERFMGKNIDVKGQDFELLPFGSGRRMCPGYNLGLKVIQSSLANLLHG 257

[Close]

BLAST of MC08g2278 vs. ExPASy TrEMBL
Match: A0A6J1GXG6 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=1)

HSP 1 Score: 194 bits (494), Expect = 2.09e-57
Identity = 96/121 (79.34%), Postives = 104/121 (85.95%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+A+L YI+AI KE MRL  V PMLVPR+ REDCQIAGYDIAKGTRVLVNVWTIGR
Sbjct: 349 VEEKDIASLPYIDAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGR 408

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN NEF PERF G +IDVKGQ FELLPF S RRMCP Y+LG+KV+QSSLANLLHG
Sbjct: 409 DPAVWENPNEFSPERFMGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHG 468

[Close]

BLAST of MC08g2278 vs. ExPASy TrEMBL
Match: A0A6J1IRR8 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1)

HSP 1 Score: 191 bits (486), Expect = 3.18e-56
Identity = 94/121 (77.69%), Postives = 103/121 (85.12%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKDMA+L YI+AI KE MRL  V PMLVPR+ REDCQIAGYDI KGTRVLVNVWTIGR
Sbjct: 349 VEEKDMASLPYIDAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIVKGTRVLVNVWTIGR 408

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN +EF PERF G +IDVKGQ FELLPF S RRMCP Y+LG+KV+Q+SLANLLHG
Sbjct: 409 DPAVWENPSEFSPERFMGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQTSLANLLHG 468

[Close]

BLAST of MC08g2278 vs. ExPASy TrEMBL
Match: A0A6J1DF57 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111020252 PE=3 SV=1)

HSP 1 Score: 191 bits (485), Expect = 4.14e-56
Identity = 94/121 (77.69%), Postives = 103/121 (85.12%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEEKD+ NL YI+AI KE MRL  V PMLVPR+SREDC+IAGYDIAK TR+LVNVWTIGR
Sbjct: 345 VEEKDITNLPYIDAIAKETMRLHPVAPMLVPRMSREDCKIAGYDIAKDTRILVNVWTIGR 404

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN NEF PERF G +IDVKGQ FELLPF S RRMCP Y+LG+KV+QSSLANLLHG
Sbjct: 405 DPEVWENPNEFNPERFIGKSIDVKGQDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHG 464

[Close]

BLAST of MC08g2278 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 131.7 bits (330), Expect = 3.9e-31
Identity = 61/121 (50.41%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           V+E D++ L Y+ A++KE  RL    P+LVPR +  D ++ G+ + K T+VLVNVW IGR
Sbjct: 351 VQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGR 410

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWEN ++F PERF G +IDVKG+ +EL PF   RR+CP   L VK V   LA+LL+ 
Sbjct: 411 DPSVWENPSQFEPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYS 470

Query: 121 F 122
           F
Sbjct: 471 F 471

[Close]

BLAST of MC08g2278 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 129.0 bits (323), Expect = 2.5e-30
Identity = 60/121 (49.59%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEE D++ L Y+ A++KE  RL    P+LVPR +  D ++ G+ + K T+V VNVW IGR
Sbjct: 352 VEESDISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGR 411

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VWENS+ F PERF G +ID++G+ +EL PF + RR+CP   L VK V   LA+LL+ 
Sbjct: 412 DPNVWENSSRFKPERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYS 471

Query: 121 F 122
           F
Sbjct: 472 F 472

[Close]

BLAST of MC08g2278 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 127.9 bits (320), Expect = 5.6e-30
Identity = 58/121 (47.93%), Postives = 83/121 (68.60%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VEE D++ L Y+ A++KE  RL    P+L+PR +  D +I G+ + K T+VLVNVW IGR
Sbjct: 351 VEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGR 410

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  VW+N ++F PERF G ++DV+G+ +EL PF + RR+CP   L +K V   LA+LL+ 
Sbjct: 411 DPSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYS 470

Query: 121 F 122
           F
Sbjct: 471 F 471

[Close]

BLAST of MC08g2278 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 126.7 bits (317), Expect = 1.3e-29
Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           VE+ D+ NL Y+ AIIKE +RL   GP+L PR + EDC +AGY +  GTR++VNVW I R
Sbjct: 361 VEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQR 420

Query: 61  DHVVWENSNEFIPERFF---GNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANL 120
           D  V+   NEF PERF        DV+GQ FEL+PF S RR CP  +L ++V+   LA  
Sbjct: 421 DPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARF 480

Query: 121 LHGF 122
           LH F
Sbjct: 481 LHSF 484

[Close]

BLAST of MC08g2278 vs. TAIR 10
Match: AT3G26150.1 (cytochrome P450, family 71, subfamily B, polypeptide 16 )

HSP 1 Score: 124.8 bits (312), Expect = 4.8e-29
Identity = 56/121 (46.28%), Postives = 79/121 (65.29%), Query Frame = 0


Query: 1   VEEKDMANLTYINAIIKEMMRLLLVGPMLVPRLSREDCQIAGYDIAKGTRVLVNVWTIGR 60
           + ++D+  + ++N +IKE  RL  V P+L+PR +    ++ GYDI    R+LVN W IGR
Sbjct: 345 ITKEDLNKVPFLNMVIKETFRLHPVAPLLLPRETMTHIKVQGYDIPPKRRILVNTWAIGR 404

Query: 61  DHVVWENSNEFIPERFFGNNIDVKGQYFELLPFRSQRRMCPRYNLGVKVVQSSLANLLHG 120
           D  +W N  EF PERF  N +D +GQ+FELLPF S RR+CP   LG+ +V+  L NLL+ 
Sbjct: 405 DPTLWINPEEFNPERFINNPVDYRGQHFELLPFGSGRRICPGMGLGITIVELGLLNLLYF 464

Query: 121 F 122
           F
Sbjct: 465 F 465

The following BLAST results are available for this feature:

BLAST of MC08g2278 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0N7F297	3.1e-33	52.89	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
A0A068Q5V6	4.5e-32	52.07	Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1	[more]
A0A1D6F9Y9	8.5e-31	51.59	Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1	[more]
A0A1D6HSP4	2.5e-30	51.61	Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1	[more]
B4FVP3	3.2e-30	52.50	Zealexin A1 synthase OS=Zea mays OX=4577 GN=CYP71Z18 PE=1 SV=1	[more]

BLAST of MC08g2278 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150738.1	4.54e-60	81.82	cytochrome P450 71A1-like [Momordica charantia]	[more]
KAG7018217.1	4.32e-57	79.34	Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022956310.1	4.32e-57	79.34	cytochrome P450 71A1-like [Cucurbita moschata]	[more]
XP_038876425.1	3.17e-56	79.34	trimethyltridecatetraene synthase-like [Benincasa hispida]	[more]
XP_023526994.1	4.68e-56	77.69	cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC08g2278 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DA87	2.20e-60	81.82	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111018796 PE=3 SV...	[more]
A0A0A0LCL6	7.96e-58	78.51	Flavonoid 3-hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_3G643770 PE=3 SV=1	[more]
A0A6J1GXG6	2.09e-57	79.34	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=...	[more]
A0A6J1IRR8	3.18e-56	77.69	cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1	[more]
A0A6J1DF57	4.14e-56	77.69	cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111020252 PE=3 SV...	[more]

BLAST of MC08g2278 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45550.1	3.9e-31	50.41	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]
AT2G45570.1	2.5e-30	49.59	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45560.1	5.6e-30	47.93	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT4G31940.1	1.3e-29	50.81	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT3G26150.1	4.8e-29	46.28	cytochrome P450, family 71, subfamily B, polypeptide 16	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 90..100 score: 42.07 coord: 100..121 score: 23.17 coord: 13..31 score: 36.08 coord: 54..78 score: 33.09
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..118 e-value: 3.8E-28 score: 98.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..121 e-value: 3.6E-34 score: 120.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..121
None	No IPR available	PANTHER	PTHR47944	CYTOCHROME P450 98A9	coord: 1..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2278.1	MC08g2278.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen