MC08g2275 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2275
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	miraculin-like
Location	MC08: 31551214 .. 31551789 (+)
RNA-Seq Expression	MC08g2275
Synteny	MC08g2275

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTCTCTTGCCATCTTGAGCTCCCTTTGCCTGATCTTCATGGCCATATCTTCATCCACAGCTCAACCTCCTCCGGTGCTCGACACCAACGGCCAAGCTCTACGACGCGGTATTGAGTACTACATCAAGCCCGCGATCACCGACGTTGCAGGCAATCTCACCCTGAAAAGTCGTAGCCGTGCCCCTTGCCCGCTCTACGTTGGCCAGGAACCGGTTGCTTCGATGGAGATCGGTCTCCCAGTCATCTTCACCCCCTCCGCCGCGGACGAGGATATAATAAGAGAAGGCATGGATTTCAAGGTTGTGTTCCAGGCGTCGTCGACGTGCATAATGTTGACACAATGGAGAGTGGAGGAGACAGAGGCTGCCACAGGGAGGAGGCTGGTGGGGACCGGGGACGACAACGGCCCGACCGGGATGTTCAGGATCGACAGGAGCAACCTCGGAGTTTACAATATAGGATGGTGTCCCGAGATGTTTGGGAGACCAAGGTGTGGAAGTGCAGGCATTTTGGTTGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAAA

mRNA sequence

AAGTCTCTTGCCATCTTGAGCTCCCTTTGCCTGATCTTCATGGCCATATCTTCATCCACAGCTCAACCTCCTCCGGTGCTCGACACCAACGGCCAAGCTCTACGACGCGGTATTGAGTACTACATCAAGCCCGCGATCACCGACGTTGCAGGCAATCTCACCCTGAAAAGTCGTAGCCGTGCCCCTTGCCCGCTCTACGTTGGCCAGGAACCGGTTGCTTCGATGGAGATCGGTCTCCCAGTCATCTTCACCCCCTCCGCCGCGGACGAGGATATAATAAGAGAAGGCATGGATTTCAAGGTTGTGTTCCAGGCGTCGTCGACGTGCATAATGTTGACACAATGGAGAGTGGAGGAGACAGAGGCTGCCACAGGGAGGAGGCTGGTGGGGACCGGGGACGACAACGGCCCGACCGGGATGTTCAGGATCGACAGGAGCAACCTCGGAGTTTACAATATAGGATGGTGTCCCGAGATGTTTGGGAGACCAAGGTGTGGAAGTGCAGGCATTTTGGTTGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAAA

Coding sequence (CDS)

AAGTCTCTTGCCATCTTGAGCTCCCTTTGCCTGATCTTCATGGCCATATCTTCATCCACAGCTCAACCTCCTCCGGTGCTCGACACCAACGGCCAAGCTCTACGACGCGGTATTGAGTACTACATCAAGCCCGCGATCACCGACGTTGCAGGCAATCTCACCCTGAAAAGTCGTAGCCGTGCCCCTTGCCCGCTCTACGTTGGCCAGGAACCGGTTGCTTCGATGGAGATCGGTCTCCCAGTCATCTTCACCCCCTCCGCCGCGGACGAGGATATAATAAGAGAAGGCATGGATTTCAAGGTTGTGTTCCAGGCGTCGTCGACGTGCATAATGTTGACACAATGGAGAGTGGAGGAGACAGAGGCTGCCACAGGGAGGAGGCTGGTGGGGACCGGGGACGACAACGGCCCGACCGGGATGTTCAGGATCGACAGGAGCAACCTCGGAGTTTACAATATAGGATGGTGTCCCGAGATGTTTGGGAGACCAAGGTGTGGAAGTGCAGGCATTTTGGTTGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAAA

Protein sequence

KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGSAFPFEFVK

Homology

[Close]

BLAST of MC08g2275 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.3e-28
Identity = 81/202 (40.10%), Postives = 115/202 (56.93%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
           ++L IL+ +CL      ++T     VLDT G+ +    EY+I+P IT+  G  T+ SR+ 
Sbjct: 8   RTLIILAHVCLFI----TTTTIAQFVLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSRNE 67

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAA--DEDIIREGMDFKVVFQASSTCIMLTQWRVE 120
           + CPL+VG E +  +  GL V FTP A   D D +R   D ++ FQASS+C+  T+WR+ 
Sbjct: 68  S-CPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLG 127

Query: 121 ETEAATGRRLVGTGDDNGPTG----MFRIDRSNL-GVYNIGWCPEMF---GRPRCGSAGI 180
           E +  +GRRL+ TG D+   G     FRI  + L G+YNI WCP       +  CG+  +
Sbjct: 128 EKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDM 187

Query: 181 LVEDGVRLLALDGSAFPFEFVK 193
           L E+G  LLALDG   P  F K
Sbjct: 188 LNENGKILLALDGGPLPLVFQK 203

[Close]

BLAST of MC08g2275 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-28
Identity = 80/201 (39.80%), Postives = 122/201 (60.70%), Query Frame = 0


Query: 2   SLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRA 61
           ++ IL+++ L  + +++S AQ   V+DT+G+ +    +Y+I+PAIT   G+LTL +R+  
Sbjct: 7   TIFILANVWL--LVVTTSIAQ--FVIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRN-- 66

Query: 62  PCPLYVGQEPVASMEIGLPVIFTP--SAADEDIIREGMDFKVVFQASSTCIMLTQWRVEE 121
            CP  VG +P A    G  V+ +P  S  +ED +R G D +V+FQA ++C   T+WR+ E
Sbjct: 67  SCPFNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGE 126

Query: 122 TEAATGRRLVGTGDDNGPTG----MFRIDRS-NLGVYNIGWCPEMF---GRPRCGSAGIL 181
            +A TGRR + TG D+   G     FRI ++ + G++NI WCP       +  CG+ GI+
Sbjct: 127 RDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIV 186

Query: 182 VEDGVRLLALDGSAFPFEFVK 193
            E+G  LLALDGSA P  F K
Sbjct: 187 RENGKILLALDGSALPVAFQK 199

[Close]

BLAST of MC08g2275 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.4e-27
Identity = 79/201 (39.30%), Postives = 112/201 (55.72%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
           +SL I     +  +  ++S AQ   V+DT+G+ +    EY+I+PAIT   G  TL +   
Sbjct: 4   RSLTIFILAHVWLLMATTSIAQ--FVIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT-GN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAA--DEDIIREGMDFKVVFQASSTCIMLTQWRVE 120
            PCPL+VG +      +G+ V FTP A   D+D +R   D +V F  S++C   T WR+ 
Sbjct: 64  GPCPLHVGLDNTEG-TLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLG 123

Query: 121 ETEAATGRRLVGTGDDNG---PTGMFRIDRSNL-GVYNIGWCPE---MFGRPRCGSAGIL 180
           E +A +GRRL+ TG DNG       FRI ++   G YNI WCP       + +CG+ G++
Sbjct: 124 EKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVI 183

Query: 181 VEDGVRLLALDGSAFPFEFVK 193
            E+G  LLALDG A P  F K
Sbjct: 184 RENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of MC08g2275 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 92.4 bits (228), Expect = 5.9e-18
Identity = 62/192 (32.29%), Postives = 92/192 (47.92%), Query Frame = 0


Query: 16  ISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKS---RSRAPCPLYVGQEPV 75
           +S++ + P PVLD +G+ LR G  YYI P + D  G LT+ +        CP  V Q   
Sbjct: 26  LSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR- 85

Query: 76  ASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTC--IMLTQWRVEETEAATGRRLVG 135
             ++   P+ F P    ED++R   D  + F A   C     T WR+++ + +TG+  V 
Sbjct: 86  KEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVT 145

Query: 136 TGDDNGPTG------MFRIDR-SNLGVYNIGWCPEMFG--RPRCGSAGILVED-GVRLLA 193
            G   G  G       F+I+     G Y + +CP + G  + +CG  GI ++  G R LA
Sbjct: 146 IGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLA 205

[Close]

BLAST of MC08g2275 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 9.3e-16
Identity = 69/182 (37.91%), Postives = 88/182 (48.35%), Query Frame = 0


Query: 6   LSSLCLIFMAIS-SSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCP 65
           L  + L  +AIS S +A PPPV DT G  L     YY+ PA     G LT+  R   PCP
Sbjct: 6   LPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-LPCP 65

Query: 66  LYVGQEPVASMEIGLPVIFTP---SAADED-IIREGMDFKVVFQASSTCIMLTQWRVEET 125
           L V QE       G PV FTP   +AA ED  IR   D ++ F A++ C+  T+W V + 
Sbjct: 66  LLVAQE-TDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGD- 125

Query: 126 EAATGRRLVGTGDDNGPT-----GMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDG 178
           E  TG R V TG   GP+       FR+++   G Y +  C     R  C   G+   DG
Sbjct: 126 EPLTGARRVVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSC-----RDSCQDLGV-SRDG 177

[Close]

BLAST of MC08g2275 vs. NCBI nr
Match: XP_022150789.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 385 bits (989), Expect = 7.42e-135
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
           KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR
Sbjct: 5   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 64

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEPVASMEIGLPVIFTP AADEDIIREGMDFKVVFQASSTCIMLTQWRVEET
Sbjct: 65  APCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 124

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA
Sbjct: 125 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 184

Query: 181 LDGSAFPFEFVK 192
           LDGSAFPFEFVK
Sbjct: 185 LDGSAFPFEFVK 196

[Close]

BLAST of MC08g2275 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 303 bits (775), Expect = 2.67e-102
Identity = 155/192 (80.73%), Postives = 166/192 (86.46%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
            SLAI S L L+FMAI+S TAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSRS 
Sbjct: 4   NSLAIFSFL-LVFMAITS-TAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEP+AS  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+E 
Sbjct: 64  APCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEM 123

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLA
Sbjct: 124 ESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 183

Query: 181 LDGSAFPFEFVK 192
           LDG+AFPFEFVK
Sbjct: 184 LDGNAFPFEFVK 192

[Close]

BLAST of MC08g2275 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298 bits (762), Expect = 2.56e-100
Identity = 153/192 (79.69%), Postives = 164/192 (85.42%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
            SLAI S L L+FMAI+S TAQ PPVLDT+GQ L+RG+EYYIKPAITDVAGNLTLKSRS 
Sbjct: 4   NSLAIFSYL-LVFMAITS-TAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+E 
Sbjct: 64  APCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEM 123

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ATGRR VG G+DN PTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLA
Sbjct: 124 ESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 183

Query: 181 LDGSAFPFEFVK 192
           LDG+AFPFEFVK
Sbjct: 184 LDGNAFPFEFVK 192

[Close]

BLAST of MC08g2275 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 297 bits (761), Expect = 3.63e-100
Identity = 152/192 (79.17%), Postives = 165/192 (85.94%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
            SLAI S L L+F+AI+S TAQ PPVLDT+GQ LRRG+EYYIKPAIT+VAGNLTLK+RS 
Sbjct: 4   NSLAIFSYL-LVFLAITS-TAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+E 
Sbjct: 64  APCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEM 123

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLA
Sbjct: 124 ESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 183

Query: 181 LDGSAFPFEFVK 192
           LDG+AFPFEFVK
Sbjct: 184 LDGNAFPFEFVK 192

[Close]

BLAST of MC08g2275 vs. NCBI nr
Match: KAG7030262.1 (hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 296 bits (757), Expect = 8.79e-100
Identity = 147/179 (82.12%), Postives = 157/179 (87.71%), Query Frame = 0


Query: 14  MAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCPLYVGQEPVA 73
           MAI+S TAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSRS APCPLYVGQEP+A
Sbjct: 1   MAITS-TAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIA 60

Query: 74  SMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAATGRRLVGTGD 133
           S  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+E E+ATGRR VG G+
Sbjct: 61  STNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGN 120

Query: 134 DNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGSAFPFEFVK 192
           DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLALDG+AFPFEFVK
Sbjct: 121 DNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVK 177

[Close]

BLAST of MC08g2275 vs. ExPASy TrEMBL
Match: A0A6J1DB43 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1)

HSP 1 Score: 385 bits (989), Expect = 3.59e-135
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
           KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR
Sbjct: 5   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 64

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEPVASMEIGLPVIFTP AADEDIIREGMDFKVVFQASSTCIMLTQWRVEET
Sbjct: 65  APCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 124

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA
Sbjct: 125 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 184

Query: 181 LDGSAFPFEFVK 192
           LDGSAFPFEFVK
Sbjct: 185 LDGSAFPFEFVK 196

[Close]

BLAST of MC08g2275 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 303 bits (775), Expect = 1.29e-102
Identity = 155/192 (80.73%), Postives = 166/192 (86.46%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
            SLAI S L L+FMAI+S TAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSRS 
Sbjct: 4   NSLAIFSFL-LVFMAITS-TAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEP+AS  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+E 
Sbjct: 64  APCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEM 123

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLA
Sbjct: 124 ESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 183

Query: 181 LDGSAFPFEFVK 192
           LDG+AFPFEFVK
Sbjct: 184 LDGNAFPFEFVK 192

[Close]

BLAST of MC08g2275 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 297 bits (761), Expect = 1.76e-100
Identity = 152/192 (79.17%), Postives = 165/192 (85.94%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
            SLAI S L L+F+AI+S TAQ PPVLDT+GQ LRRG+EYYIKPAIT+VAGNLTLK+RS 
Sbjct: 4   NSLAIFSYL-LVFLAITS-TAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPLYVGQEPVAS  IGLPV FTP  A EDII E M F V FQASSTCI  TQWRV+E 
Sbjct: 64  APCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEM 123

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ATGRR VG G+DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLA
Sbjct: 124 ESATGRRFVGVGNDNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 183

Query: 181 LDGSAFPFEFVK 192
           LDG+AFPFEFVK
Sbjct: 184 LDGNAFPFEFVK 192

[Close]

BLAST of MC08g2275 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 268 bits (684), Expect = 9.32e-89
Identity = 135/192 (70.31%), Postives = 150/192 (78.12%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
           KSLAI    CL FMAI+S TAQ PPVLDTNGQ LRRG+EYYI PAITDV GNLTLKSRS 
Sbjct: 4   KSLAIFGYACL-FMAITS-TAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSN 63

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPL+VGQEPV S  IGLPV F P+ A +DII EG    +VF+A STC   TQWRV+ T
Sbjct: 64  APCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDAT 123

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ TGRR VG GD++GP G+F I R N G YNI WCP M GRPRCG AGILVE+GVRL+A
Sbjct: 124 ESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 183

Query: 181 LDGSAFPFEFVK 192
           LDG AFPFEF+K
Sbjct: 184 LDGDAFPFEFIK 192

[Close]

BLAST of MC08g2275 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 268 bits (684), Expect = 9.97e-89
Identity = 135/192 (70.31%), Postives = 150/192 (78.12%), Query Frame = 0


Query: 1   KSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSR 60
           KSLAI    CL FMAI+S TAQ PPVLDTNGQ LRRG+EYYI PAITDV GNLTLKSRS 
Sbjct: 6   KSLAIFGYACL-FMAITS-TAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSN 65

Query: 61  APCPLYVGQEPVASMEIGLPVIFTPSAADEDIIREGMDFKVVFQASSTCIMLTQWRVEET 120
           APCPL+VGQEPV S  IGLPV F P+ A +DII EG    +VF+A STC   TQWRV+ T
Sbjct: 66  APCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDAT 125

Query: 121 EAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLA 180
           E+ TGRR VG GD++GP G+F I R N G YNI WCP M GRPRCG AGILVE+GVRL+A
Sbjct: 126 ESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 185

Query: 181 LDGSAFPFEFVK 192
           LDG AFPFEF+K
Sbjct: 186 LDGDAFPFEFIK 194

The following BLAST results are available for this feature:

BLAST of MC08g2275 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV1	1.3e-28	40.10	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
G7LCV7	2.1e-28	39.80	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	2.4e-27	39.30	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	5.9e-18	32.29	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P29421	9.3e-16	37.91	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of MC08g2275 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150789.1	7.42e-135	99.48	miraculin-like [Momordica charantia]	[more]
XP_022946294.1	2.67e-102	80.73	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_023545477.1	2.56e-100	79.69	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022999136.1	3.63e-100	79.17	miraculin-like [Cucurbita maxima]	[more]
KAG7030262.1	8.79e-100	82.12	hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of MC08g2275 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DB43	3.59e-135	99.48	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1	[more]
A0A6J1G3G3	1.29e-102	80.73	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	1.76e-100	79.17	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A5A7VDV8	9.32e-89	70.31	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A5D3D468	9.97e-89	70.31	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]

BLAST of MC08g2275 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 25..192 e-value: 4.8E-26 score: 102.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 26..192 e-value: 8.8E-39 score: 133.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 6..192
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 26..42
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 25..192 e-value: 2.4917E-36 score: 122.836
None	No IPR available	GENE3D	2.80.10.50		coord: 20..192 e-value: 1.1E-42 score: 147.7
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 6..192
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 24..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2275.1	MC08g2275.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0050896	response to stimulus
molecular_function	GO:0004866	endopeptidase inhibitor activity