MC08g2239 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2239
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionheavy metal-associated isoprenylated plant protein 36
LocationMC08: 31156850 .. 31158549 (-)
RNA-Seq ExpressionMC08g2239
SyntenyMC08g2239
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGCATCTCCCATCTCTCTCTCTCAAGAACAGAGCTCGAAGCTTCAGTGCATTTAATGGCAGAACCAGAAGCCAAGCCAGAGCCTAAAGAAGTAGAAGAAATCGCGGAACCTCCTCTCATATACAAGGTAGTTTCGATTTCGGCTACTCTTTTTTGCAAATCCATAACTCATCCATTCTTCAACCTCTCGCTATACGGTTTTTCTGTTGACGATTCTGTGATTTTTTTGTTCCTGTTTCATCAGACGTGGATTCTGAAAGTCTCTATCCACTGCGAAGCCTGCAAGAGGAAGGTGAAACGAGTTTTGAAGGACGTTGAAGGTCGATTTCGAAGTTTGATTTCATTTATTTAATCTTTCTTCCCGGTTGTTCGTCGATGAAGTTTTTTCTCACTCCTGTTCGTATTGCGATCTGTTTTCTTCGAGTTCAGTTCTTCAATGGCGTTTTTAACAGTCGCCTCTCTCTCTTGATCGAGTATCACTCTTCGGAACTTGCTATTATTCCTTTCTTAGCATATTCATTTCACAATAACTTGAAAAATGATATGCAATGGCGTTTTTCACAGTTTACCTCTCTATAAATCGCTGTTTTCTCTGGAATATGGATTAGAAAATATCTGCTGATTTTGAATCCGACTATTCAGGAGTGTATGAAACGGATATCGATTTAAAGCAACAGAAAGTGGTTGTGAAAGGGAACGTCGAATCGGAAACTTTGATAAAGAAATTGTTGAAGACAGGGAAGCACGCGGAGCTGTGGCCGGAGAAGTCGGATTCAAAAGGAAAGAAGAAAGGAAAATCGAAGAAGAAAGAGAAACAGAGCGACTCCGAAAGCAGCGGCAATGAAGACGAAAAAGAGAAGGTGAAATTCGAGGTTCAAGTCACGGACCCGACGAAAAATGGAGAGGCTGCCGGAAAAATCAACGACGACGGTGGCCAAGCGAAGCAACCTGAAACTTCAACGACCGGTGGCGGCCAGACAAGCGGCGGAGGTAAGAAGAAGAAAAAGAAGAAGAAGAAGAAGAGCAGCGGCAATGCCGGCGGTGAATCGGCCGGTGATGCTCCGGCGAACGGAGGCGCACCGCCTGGTCCAGCTCCGGGTCATCCAGAACCCGGGCCACAACCCGCACCCGGCCCGTCTCCAATTCTAGCCAACGAGAGCCGTCCACGTCACTTCGTGACTCAATACCCACCGCATTACACTACACACCCACTTTATTCCCTGCGGTACGGTACAGCATACGCAACATGTTCTCAACCTCATAGCGAATCACATTACGCCACACCGCAGCAGTATTCCTATGCGTACGTTCATCCAGGTGTACAGACGGAGTTTGATCCTCCACCGTCCGATTACGAGCCATATTTATCTCAGCCGTCCGGTTCTCTCGAGATATTCAGCGACGAAAATCCTAATGGCTGCTCAGTCGTGTGAGGGAATTTTCCTTTTTTGCTAAGAAATTACCATATTGGGCAACCTAAATGCGAAATCTTAAACTACTTGTAAGAGTATGTGGAGGGGTAGTTTTGTAATGTACAGGTGGCTTCTATTTTGATATCAGCGCTTTTTGGTGGCGATGGTACATTTTGAAATGTTGAAACTCTCTTTCTCTCCTTCCCAGCCTCTCGTGTAAATAAATGAGGTTTCCTTTCCAATAAAAATAAACAGATGAGATTTTTATACTAAAATATATGTCATT

mRNA sequence

CAAAGCATCTCCCATCTCTCTCTCTCAAGAACAGAGCTCGAAGCTTCAGTGCATTTAATGGCAGAACCAGAAGCCAAGCCAGAGCCTAAAGAAGTAGAAGAAATCGCGGAACCTCCTCTCATATACAAGACGTGGATTCTGAAAGTCTCTATCCACTGCGAAGCCTGCAAGAGGAAGGTGAAACGAGTTTTGAAGGACGTTGAAGGAGTGTATGAAACGGATATCGATTTAAAGCAACAGAAAGTGGTTGTGAAAGGGAACGTCGAATCGGAAACTTTGATAAAGAAATTGTTGAAGACAGGGAAGCACGCGGAGCTGTGGCCGGAGAAGTCGGATTCAAAAGGAAAGAAGAAAGGAAAATCGAAGAAGAAAGAGAAACAGAGCGACTCCGAAAGCAGCGGCAATGAAGACGAAAAAGAGAAGGTGAAATTCGAGGTTCAAGTCACGGACCCGACGAAAAATGGAGAGGCTGCCGGAAAAATCAACGACGACGGTGGCCAAGCGAAGCAACCTGAAACTTCAACGACCGGTGGCGGCCAGACAAGCGGCGGAGGTAAGAAGAAGAAAAAGAAGAAGAAGAAGAAGAGCAGCGGCAATGCCGGCGGTGAATCGGCCGGTGATGCTCCGGCGAACGGAGGCGCACCGCCTGGTCCAGCTCCGGGTCATCCAGAACCCGGGCCACAACCCGCACCCGGCCCGTCTCCAATTCTAGCCAACGAGAGCCGTCCACGTCACTTCGTGACTCAATACCCACCGCATTACACTACACACCCACTTTATTCCCTGCGGTACGGTACAGCATACGCAACATGTTCTCAACCTCATAGCGAATCACATTACGCCACACCGCAGCAGTATTCCTATGCGTACGTTCATCCAGGTGTACAGACGGAGTTTGATCCTCCACCGTCCGATTACGAGCCATATTTATCTCAGCCGTCCGGTTCTCTCGAGATATTCAGCGACGAAAATCCTAATGGCTGCTCAGTCGTGTGAGGGAATTTTCCTTTTTTGCTAAGAAATTACCATATTGGGCAACCTAAATGCGAAATCTTAAACTACTTGTAAGAGTATGTGGAGGGGTAGTTTTGTAATGTACAGGTGGCTTCTATTTTGATATCAGCGCTTTTTGGTGGCGATGGTACATTTTGAAATGTTGAAACTCTCTTTCTCTCCTTCCCAGCCTCTCGTGTAAATAAATGAGGTTTCCTTTCCAATAAAAATAAACAGATGAGATTTTTATACTAAAATATATGTCATT

Coding sequence (CDS)

CAAAGCATCTCCCATCTCTCTCTCTCAAGAACAGAGCTCGAAGCTTCAGTGCATTTAATGGCAGAACCAGAAGCCAAGCCAGAGCCTAAAGAAGTAGAAGAAATCGCGGAACCTCCTCTCATATACAAGACGTGGATTCTGAAAGTCTCTATCCACTGCGAAGCCTGCAAGAGGAAGGTGAAACGAGTTTTGAAGGACGTTGAAGGAGTGTATGAAACGGATATCGATTTAAAGCAACAGAAAGTGGTTGTGAAAGGGAACGTCGAATCGGAAACTTTGATAAAGAAATTGTTGAAGACAGGGAAGCACGCGGAGCTGTGGCCGGAGAAGTCGGATTCAAAAGGAAAGAAGAAAGGAAAATCGAAGAAGAAAGAGAAACAGAGCGACTCCGAAAGCAGCGGCAATGAAGACGAAAAAGAGAAGGTGAAATTCGAGGTTCAAGTCACGGACCCGACGAAAAATGGAGAGGCTGCCGGAAAAATCAACGACGACGGTGGCCAAGCGAAGCAACCTGAAACTTCAACGACCGGTGGCGGCCAGACAAGCGGCGGAGGTAAGAAGAAGAAAAAGAAGAAGAAGAAGAAGAGCAGCGGCAATGCCGGCGGTGAATCGGCCGGTGATGCTCCGGCGAACGGAGGCGCACCGCCTGGTCCAGCTCCGGGTCATCCAGAACCCGGGCCACAACCCGCACCCGGCCCGTCTCCAATTCTAGCCAACGAGAGCCGTCCACGTCACTTCGTGACTCAATACCCACCGCATTACACTACACACCCACTTTATTCCCTGCGGTACGGTACAGCATACGCAACATGTTCTCAACCTCATAGCGAATCACATTACGCCACACCGCAGCAGTATTCCTATGCGTACGTTCATCCAGGTGTACAGACGGAGTTTGATCCTCCACCGTCCGATTACGAGCCATATTTATCTCAGCCGTCCGGTTCTCTCGAGATATTCAGCGACGAAAATCCTAATGGCTGCTCAGTCGTGTGA

Protein sequence

QSISHLSLSRTELEASVHLMAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKNGEAAGKINDDGGQAKQPETSTTGGGQTSGGGKKKKKKKKKKSSGNAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQYPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV
Homology
BLAST of MC08g2239 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-33
Identity = 134/352 (38.07%), Postives = 170/352 (48.30%), Query Frame = 0

Query: 28  EPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGN 87
           E + V E    PL Y TW+L+VSIHCE CKRK+K++L  ++GVY T+ID+KQQKV V GN
Sbjct: 15  EQRHVFEDYPEPLRYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGN 74

Query: 88  VESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQ 147
           VE E LIKK++K G+HAELWP   ++        +KK K+ + E+SG+ED+ E       
Sbjct: 75  VEPEILIKKIMKAGRHAELWPTSMENNINNDCNYQKKPKKDNEETSGDEDDDEN------ 134

Query: 148 VTDPTKNGEAAGKINDDGG-------QAKQPETSTTGGGQTSGGG---KKKKKKKKKKSS 207
                 N    G +   GG       Q KQ  T   G  Q  G G   KKKKKKKKKKS 
Sbjct: 135 ----NNNNNGGGDVGGGGGGGGGNFDQVKQVVTFVNGQLQPQGDGAPKKKKKKKKKKKSL 194

Query: 208 GNAGGESAGDAPANGGAPPGPAPGHPEPGPQPAP-------GPSPILAN----------- 267
           GN      G     GG  P    G PE     AP       GP P L +           
Sbjct: 195 GNTTVVMEGGGGGGGGGGPPQNDGPPETVIYSAPQDHHIIHGPPPHLHHHQQQNHPYPTV 254

Query: 268 ESRPRHFVTQY--------PPHY-----TTHPLYSLRYGTAYATCSQPHSE--SHYATP- 327
            S PRH   Q         PP +      T P Y++ Y T +   +   +E  S+Y  P 
Sbjct: 255 HSPPRHHPQQMYGPGPGLPPPSFYHTQPQTAPSYTVSYNTVHGPINNGGNETASYYTAPP 314

Query: 328 ----QQYSYAYVHPGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV 332
                 YSY YV  G    ++ PP ++  Y SQPS S E  S+ NPN C V+
Sbjct: 315 SHPTSYYSYEYVDTG----YESPPPEFYSYRSQPSESFESLSEGNPNSCCVM 352

BLAST of MC08g2239 vs. ExPASy Swiss-Prot
Match: Q9C7J6 (Heavy metal-associated isoprenylated plant protein 35 OS=Arabidopsis thaliana OX=3702 GN=HIPP35 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-24
Identity = 126/374 (33.69%), Postives = 169/374 (45.19%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEE----------------IAEPPLIYKTWILKVSIHCEACKRKVKRV 79
           MA  E K E K+ E                 I   PL YK+  LKVSIHCE CKRKVK++
Sbjct: 1   MATDEMKSETKKTEHKQKQSTQIKQDLPPPTIPPLPLPYKSCTLKVSIHCEGCKRKVKKI 60

Query: 80  LKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSD----------- 139
           L  +EGV++ DID+KQ KV V G +  E L+KKL K GK+AE  PE  D           
Sbjct: 61  LTSIEGVFKVDIDVKQHKVTVIGIISPEILLKKLNKAGKNAEQLPEIPDPVDNKPKPVDP 120

Query: 140 -SKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTK----------NGEAAGKI 199
             K KKK K +K +  +++ SSG ++ ++    E    +  K           GE +G +
Sbjct: 121 KEKNKKKKKEEKVQITNEATSSGIDNPEKPGSGECDKPESEKPVDEKCLSGDGGETSGPV 180

Query: 200 ND---------DGGQAKQP-------------ETSTTGGGQTSGGGKKKKKKKKKKSSGN 259
            +         D G+ + P             +   TGG     G   KKKKKK +S   
Sbjct: 181 KEEKKDVLKEKDSGKEESPSPPADSSAPAAEKKAEDTGGAVPDNGKVGKKKKKKGQSLAT 240

Query: 260 AGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQYPP--HYTTH 319
               + G A      PP  A  +  P  Q       I    + PRH +  YP   +Y   
Sbjct: 241 TNNPTDGPARTQ-SLPPPTATDYDRPINQ--INDHHITTTNNPPRHDLYPYPAQGYYAPQ 300

Query: 320 PLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPYLSQPSGSL 332
            +Y + Y  A    S   + S+Y  P  YSYAY+H G Q      PSD  P   +PS S 
Sbjct: 301 VMYGVSYNVAQPPVS-VDAASYYTPPPPYSYAYMHNGYQ------PSDQNPCQPRPSDSF 360

BLAST of MC08g2239 vs. ExPASy Swiss-Prot
Match: F4JZL7 (Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX=3702 GN=HIPP33 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.5e-13
Identity = 56/143 (39.16%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 43  KTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGK 102
           +T +LKV+IHC+ CK+KVK++L+ +EGV+ T ID +  KV V GNV+   LIKKLLK+GK
Sbjct: 10  QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 103 HAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKNGEAAGKIN 162
           HAE+W       G  KG S   + Q +  +          +F+    D    G   G   
Sbjct: 70  HAEIW-------GAPKGGSNNNQNQPNLAN----------QFKAMQIDHGGKGGGGG--- 129

Query: 163 DDGGQAKQPETSTTGGGQTSGGG 186
             GG A   +    GGG   GGG
Sbjct: 130 -GGGPANNNKGQKIGGGGGGGGG 131

BLAST of MC08g2239 vs. ExPASy Swiss-Prot
Match: Q9M8K5 (Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX=3702 GN=HIPP32 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.8e-11
Identity = 53/149 (35.57%), Postives = 80/149 (53.69%), Query Frame = 0

Query: 43  KTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGK 102
           +T +LKV+IHC+ CK+KVK++L+ +EGV+ T ID +Q KV V G+V+   LIKKL K+GK
Sbjct: 10  QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGK 69

Query: 103 HAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKNGEAAGKIN 162
           HAE+W       G  KG +   + Q  ++  G + +               +G+A G   
Sbjct: 70  HAEIW-------GAPKGNNNPNQSQMANQFKGMQID---------------HGKAGGGGG 129

Query: 163 DDGGQAKQPETSTTGGGQTSGGGKKKKKK 192
            +    K+ + +  GGG   GGG     K
Sbjct: 130 GNNNNNKKGQKNGGGGGGGGGGGNSNAPK 136

BLAST of MC08g2239 vs. ExPASy Swiss-Prot
Match: A2RVM8 (Heavy metal-associated isoprenylated plant protein 37 OS=Arabidopsis thaliana OX=3702 GN=HIPP37 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-08
Identity = 52/166 (31.33%), Postives = 86/166 (51.81%), Query Frame = 0

Query: 43  KTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGK 102
           +T+ L+V+IHCE C +KVK++L+ +EGV    I+ + QKV V G+V+S TLI KL+K GK
Sbjct: 13  QTFSLRVNIHCEGCNKKVKKLLQRIEGVCHVKIEAEHQKVTVSGSVDSATLINKLVKAGK 72

Query: 103 HAELW--------PEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKN 162
           HAELW        P+K  +    K  ++K +KQ  ++S             ++   P   
Sbjct: 73  HAELWSPNPNQNQPQKPKTNDFIKNVNQKGQKQGSAKSG------------IEACKPKNG 132

Query: 163 GEAAGKINDDGGQAKQPETSTTGGGQTSGGGKKKKKKKKKKSSGNA 201
            + A  + ++ G   + E       + +   +++     KK+SG A
Sbjct: 133 PKGAAFVAEEDGDGSEEEDGDVQFPKPANQQQQQNVVNAKKNSGGA 166

BLAST of MC08g2239 vs. NCBI nr
Match: XP_023537434.1 (heavy metal-associated isoprenylated plant protein 36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458 bits (1178), Expect = 2.66e-159
Identity = 264/324 (81.48%), Postives = 277/324 (85.49%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQ 79
           MAEPEAKPE KE+EE AEPPL+YKTWILKVSIHCEACKRKVKRVLKDVEGVYETDID +Q
Sbjct: 1   MAEPEAKPELKEIEEHAEPPLVYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDWRQ 60

Query: 80  QKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESS----GN 139
           QKVVVKGNVESETLIKKLLKTGKHAELWPE SDSKGKKKGKSKKKEKQSDSESS    G+
Sbjct: 61  QKVVVKGNVESETLIKKLLKTGKHAELWPENSDSKGKKKGKSKKKEKQSDSESSDESSGH 120

Query: 140 EDEKEKVKFEVQVTDPTKNGEAAGKINDDG--GQAKQPETSTTGGGQTSG-GGKKKKKKK 199
           EDEKEKVK EVQ  DPTK G+ AGKI DDG  GQAKQP ++  G GQTSG GGKKKKKKK
Sbjct: 121 EDEKEKVKLEVQ--DPTKIGDTAGKITDDGSGGQAKQPGSAPVGDGQTSGSGGKKKKKKK 180

Query: 200 KKKSSG-----NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFV 259
           KKKSSG     NAGGE+ GDA A G  PPGP PG PEPGPQP  GP PILANES PRHFV
Sbjct: 181 KKKSSGGGGGGNAGGEAPGDASAIGAPPPGPTPGLPEPGPQPLTGPLPILANESSPRHFV 240

Query: 260 TQYPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYE 319
           TQY PH  THPLYS+RY TAYATCSQP  ES+YATPQQYSYA+VHPGV+ EFD PPSDYE
Sbjct: 241 TQYAPHSFTHPLYSVRYSTAYATCSQPQPESYYATPQQYSYAHVHPGVRHEFDIPPSDYE 300

Query: 320 PYLSQPSGSLEIFSDENPNGCSVV 331
           PY SQPS SLEIFSDENPNGCS+V
Sbjct: 301 PYSSQPSDSLEIFSDENPNGCSIV 322

BLAST of MC08g2239 vs. NCBI nr
Match: XP_022937460.1 (heavy metal-associated isoprenylated plant protein 36 [Cucurbita moschata])

HSP 1 Score: 454 bits (1168), Expect = 8.23e-158
Identity = 261/322 (81.06%), Postives = 276/322 (85.71%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQ 79
           MAEPEAKPE KE+EE AEPPL+YKTWILKVSIHCEACKRKVKRVLKDVEGVYETDID +Q
Sbjct: 1   MAEPEAKPELKEIEENAEPPLVYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDWRQ 60

Query: 80  QKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESS----GN 139
           QKVVVKGNVESETLIKKLLKTGKHAELWPE SDSKGKKKGKSKKKEKQSDSESS    G+
Sbjct: 61  QKVVVKGNVESETLIKKLLKTGKHAELWPENSDSKGKKKGKSKKKEKQSDSESSDESSGH 120

Query: 140 EDEKEKVKFEVQVTDPTKNGEAAGKINDDG--GQAKQPETSTTGGGQTSG-GGKKKKKKK 199
           EDEKEKVK EVQ  DPTKNG+ AGKI DDG  GQAKQP ++    GQTSG GGKKKKKKK
Sbjct: 121 EDEKEKVKLEVQ--DPTKNGDTAGKITDDGSGGQAKQPSSAPVCDGQTSGSGGKKKKKKK 180

Query: 200 KKKSSG---NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQ 259
           KKKSSG   NAGGE+ G+A A G  PPGP  G PEPGPQP  GP PI+ANES PRHFVTQ
Sbjct: 181 KKKSSGGGGNAGGEAPGEASAIGAPPPGPTQGLPEPGPQPLTGPLPIIANESSPRHFVTQ 240

Query: 260 YPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPY 319
           Y PH  THPLYS+RY TAYATCSQP  ES+YATPQQYSYA+VHPGV+ EFD PPSDYEPY
Sbjct: 241 YAPHSFTHPLYSVRYSTAYATCSQPQPESYYATPQQYSYAHVHPGVRHEFDIPPSDYEPY 300

Query: 320 LSQPSGSLEIFSDENPNGCSVV 331
            SQPS SLEIFSDENPNGCS+V
Sbjct: 301 SSQPSDSLEIFSDENPNGCSIV 320

BLAST of MC08g2239 vs. NCBI nr
Match: KAG6586249.1 (Heavy metal-associated isoprenylated plant protein 36, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021092.1 Heavy metal-associated isoprenylated plant protein 36 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 450 bits (1158), Expect = 2.73e-156
Identity = 260/322 (80.75%), Postives = 275/322 (85.40%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQ 79
           MAEPEAKPE KE+EE AEPPL+YKTWILKVSIHCEACKRKVKRVLKDVEGVYETDID +Q
Sbjct: 1   MAEPEAKPELKEIEENAEPPLVYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDWRQ 60

Query: 80  QKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESS----GN 139
           QKVVVKGNVESETLIKKLLKTGKHAELWPE SDSKGKKKGKSKKKEKQSDSESS    G+
Sbjct: 61  QKVVVKGNVESETLIKKLLKTGKHAELWPENSDSKGKKKGKSKKKEKQSDSESSDESSGH 120

Query: 140 EDEKEKVKFEVQVTDPTKNGEAAGKINDDG--GQAKQPETSTTGGGQTSG-GGKKKKKKK 199
           EDEKEKVK EVQ  DPTK G+ AGKI DDG  GQAKQ  ++  G GQTSG GGKKKKKKK
Sbjct: 121 EDEKEKVKLEVQ--DPTKIGDTAGKITDDGSGGQAKQTGSAPVGDGQTSGSGGKKKKKKK 180

Query: 200 KKKSSG---NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQ 259
           KKKSSG   NAGGE+ G+A A G  PPGP  G PEPGPQP  GP PI+ANES PRHFVTQ
Sbjct: 181 KKKSSGGGGNAGGEAPGEASAIGAPPPGPTQGLPEPGPQPLTGPLPIIANESSPRHFVTQ 240

Query: 260 YPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPY 319
           Y PH  THPLYS+RY TAYATCSQP  ES+YATPQQYSYA+VHPGV+ EFD PPSDYEPY
Sbjct: 241 YAPHSFTHPLYSVRYSTAYATCSQPQPESYYATPQQYSYAHVHPGVRHEFDIPPSDYEPY 300

Query: 320 LSQPSGSLEIFSDENPNGCSVV 331
            SQPS SLEIFSDENPNGCS+V
Sbjct: 301 SSQPSDSLEIFSDENPNGCSIV 320

BLAST of MC08g2239 vs. NCBI nr
Match: XP_022965847.1 (heavy metal-associated isoprenylated plant protein 36 [Cucurbita maxima])

HSP 1 Score: 449 bits (1155), Expect = 7.81e-156
Identity = 259/322 (80.43%), Postives = 274/322 (85.09%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQ 79
           MAEPEAK E KE+EE AEPPL+YKTWILKVSIHCEACKRKVKRVLKDVEGVYETDID +Q
Sbjct: 1   MAEPEAKLELKEIEENAEPPLVYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDWRQ 60

Query: 80  QKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESS----GN 139
           QKVVVKGNVESETLIKKLLKTGKHAELWPE SDSK KKKGKSKKKEKQSDSESS    G+
Sbjct: 61  QKVVVKGNVESETLIKKLLKTGKHAELWPENSDSKVKKKGKSKKKEKQSDSESSDESSGH 120

Query: 140 EDEKEKVKFEVQVTDPTKNGEAAGKINDDG--GQAKQPETSTTGGGQTSG-GGKKKKKKK 199
           EDEKEKVK EVQ  DPTKNG+ AGK+ DDG  GQAKQP ++  G GQT G GGKKKKKKK
Sbjct: 121 EDEKEKVKLEVQ--DPTKNGDTAGKVTDDGSGGQAKQPGSAPVGDGQTGGSGGKKKKKKK 180

Query: 200 KKKSSG---NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQ 259
           KKKSSG   NAGGE+ GDA A G  PPGP  G PEPGPQP  GP PI+ANES PRHFVTQ
Sbjct: 181 KKKSSGGGGNAGGEAPGDASAIGAPPPGPTQGPPEPGPQPLTGPLPIIANESSPRHFVTQ 240

Query: 260 YPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPY 319
           Y PH  THPLYS+RY TAYATCSQP  ES+YATPQQYSYA+VHPGV+ EFD PPSDYEPY
Sbjct: 241 YVPHSFTHPLYSVRYSTAYATCSQPQPESYYATPQQYSYAHVHPGVRHEFDIPPSDYEPY 300

Query: 320 LSQPSGSLEIFSDENPNGCSVV 331
            SQPS SLEIFSDENPNGCS+V
Sbjct: 301 SSQPSDSLEIFSDENPNGCSIV 320

BLAST of MC08g2239 vs. NCBI nr
Match: XP_038889488.1 (heavy metal-associated isoprenylated plant protein 36-like [Benincasa hispida])

HSP 1 Score: 449 bits (1156), Expect = 1.60e-155
Identity = 262/341 (76.83%), Postives = 276/341 (80.94%), Query Frame = 0

Query: 2   SISHLSLSRTELEASVHLMAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVK 61
           S SH     T+L+ASV LMAEPE KPEPKEVEE  EPPLIYKTWILKVSIHCEACKRKVK
Sbjct: 16  SHSHSHSLSTDLKASVSLMAEPEGKPEPKEVEENPEPPLIYKTWILKVSIHCEACKRKVK 75

Query: 62  RVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKS 121
           RVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK    GK KGKS
Sbjct: 76  RVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK----GKSKGKS 135

Query: 122 KKKEKQSDSESS----GNEDEKEKVKFEVQVTDPTKNGEAAGKINDDGG--QAKQPETST 181
           KKKEK SDSESS    G+EDEKEKVKFEVQ  DPTKNG+ AGKI    G  QAKQP T+ 
Sbjct: 136 KKKEKNSDSESSDESSGHEDEKEKVKFEVQ--DPTKNGDPAGKIIGGSGDSQAKQPGTAP 195

Query: 182 TGGGQTSGGGKKKKKKKKKKSSGNAGG-----ESAGDAPANGGAPPGPAPGHPEPGPQPA 241
            G GQTSGGG KKKKKKKKK SG  GG     E+ G  PANG  P G  PGH EPGPQPA
Sbjct: 196 AGDGQTSGGGGKKKKKKKKKKSGGGGGGNASVEAPGGHPANGVPPAGADPGHLEPGPQPA 255

Query: 242 PGPSPILANESRPRHFVTQYPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAY 301
           PGP PI+ANES PRHFVTQY PHY  HP+YS+RY TAYATCSQP  ES+YATPQQYSYAY
Sbjct: 256 PGPLPIIANESPPRHFVTQYAPHYIAHPVYSVRYSTAYATCSQPQRESYYATPQQYSYAY 315

Query: 302 VHPGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV 331
           VHPGV+ + D PPSD+EPY S PS SLEIFSDENPNGCS+V
Sbjct: 316 VHPGVRPQLDSPPSDFEPYSSHPSDSLEIFSDENPNGCSIV 350

BLAST of MC08g2239 vs. ExPASy TrEMBL
Match: A0A6J1FGQ1 (heavy metal-associated isoprenylated plant protein 36 OS=Cucurbita moschata OX=3662 GN=LOC111443865 PE=4 SV=1)

HSP 1 Score: 454 bits (1168), Expect = 3.98e-158
Identity = 261/322 (81.06%), Postives = 276/322 (85.71%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQ 79
           MAEPEAKPE KE+EE AEPPL+YKTWILKVSIHCEACKRKVKRVLKDVEGVYETDID +Q
Sbjct: 1   MAEPEAKPELKEIEENAEPPLVYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDWRQ 60

Query: 80  QKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESS----GN 139
           QKVVVKGNVESETLIKKLLKTGKHAELWPE SDSKGKKKGKSKKKEKQSDSESS    G+
Sbjct: 61  QKVVVKGNVESETLIKKLLKTGKHAELWPENSDSKGKKKGKSKKKEKQSDSESSDESSGH 120

Query: 140 EDEKEKVKFEVQVTDPTKNGEAAGKINDDG--GQAKQPETSTTGGGQTSG-GGKKKKKKK 199
           EDEKEKVK EVQ  DPTKNG+ AGKI DDG  GQAKQP ++    GQTSG GGKKKKKKK
Sbjct: 121 EDEKEKVKLEVQ--DPTKNGDTAGKITDDGSGGQAKQPSSAPVCDGQTSGSGGKKKKKKK 180

Query: 200 KKKSSG---NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQ 259
           KKKSSG   NAGGE+ G+A A G  PPGP  G PEPGPQP  GP PI+ANES PRHFVTQ
Sbjct: 181 KKKSSGGGGNAGGEAPGEASAIGAPPPGPTQGLPEPGPQPLTGPLPIIANESSPRHFVTQ 240

Query: 260 YPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPY 319
           Y PH  THPLYS+RY TAYATCSQP  ES+YATPQQYSYA+VHPGV+ EFD PPSDYEPY
Sbjct: 241 YAPHSFTHPLYSVRYSTAYATCSQPQPESYYATPQQYSYAHVHPGVRHEFDIPPSDYEPY 300

Query: 320 LSQPSGSLEIFSDENPNGCSVV 331
            SQPS SLEIFSDENPNGCS+V
Sbjct: 301 SSQPSDSLEIFSDENPNGCSIV 320

BLAST of MC08g2239 vs. ExPASy TrEMBL
Match: A0A6J1HLE7 (heavy metal-associated isoprenylated plant protein 36 OS=Cucurbita maxima OX=3661 GN=LOC111465607 PE=4 SV=1)

HSP 1 Score: 449 bits (1155), Expect = 3.78e-156
Identity = 259/322 (80.43%), Postives = 274/322 (85.09%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQ 79
           MAEPEAK E KE+EE AEPPL+YKTWILKVSIHCEACKRKVKRVLKDVEGVYETDID +Q
Sbjct: 1   MAEPEAKLELKEIEENAEPPLVYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDWRQ 60

Query: 80  QKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESS----GN 139
           QKVVVKGNVESETLIKKLLKTGKHAELWPE SDSK KKKGKSKKKEKQSDSESS    G+
Sbjct: 61  QKVVVKGNVESETLIKKLLKTGKHAELWPENSDSKVKKKGKSKKKEKQSDSESSDESSGH 120

Query: 140 EDEKEKVKFEVQVTDPTKNGEAAGKINDDG--GQAKQPETSTTGGGQTSG-GGKKKKKKK 199
           EDEKEKVK EVQ  DPTKNG+ AGK+ DDG  GQAKQP ++  G GQT G GGKKKKKKK
Sbjct: 121 EDEKEKVKLEVQ--DPTKNGDTAGKVTDDGSGGQAKQPGSAPVGDGQTGGSGGKKKKKKK 180

Query: 200 KKKSSG---NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQ 259
           KKKSSG   NAGGE+ GDA A G  PPGP  G PEPGPQP  GP PI+ANES PRHFVTQ
Sbjct: 181 KKKSSGGGGNAGGEAPGDASAIGAPPPGPTQGPPEPGPQPLTGPLPIIANESSPRHFVTQ 240

Query: 260 YPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPY 319
           Y PH  THPLYS+RY TAYATCSQP  ES+YATPQQYSYA+VHPGV+ EFD PPSDYEPY
Sbjct: 241 YVPHSFTHPLYSVRYSTAYATCSQPQPESYYATPQQYSYAHVHPGVRHEFDIPPSDYEPY 300

Query: 320 LSQPSGSLEIFSDENPNGCSVV 331
            SQPS SLEIFSDENPNGCS+V
Sbjct: 301 SSQPSDSLEIFSDENPNGCSIV 320

BLAST of MC08g2239 vs. ExPASy TrEMBL
Match: A0A1S3BYY5 (uncharacterized protein LOC103494708 OS=Cucumis melo OX=3656 GN=LOC103494708 PE=4 SV=1)

HSP 1 Score: 428 bits (1101), Expect = 1.54e-147
Identity = 258/339 (76.11%), Postives = 272/339 (80.24%), Query Frame = 0

Query: 7   SLSRTE-LEASVHLMAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLK 66
           SLSRTE L+ SV LMAE E KPEPKEVEE  EPPLIYKTWILKVSIHCEACKRKVKRVLK
Sbjct: 14  SLSRTEQLKPSVPLMAEQEPKPEPKEVEENLEPPLIYKTWILKVSIHCEACKRKVKRVLK 73

Query: 67  DVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKE 126
           D+EGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK    GK KGKSKKKE
Sbjct: 74  DIEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK----GKSKGKSKKKE 133

Query: 127 KQSDSESSG----NEDEKEKVKFEVQVTDPTKNGEAAGKINDDGG--QAKQPETSTTGGG 186
           K SDSESSG    +EDEKEKVKFEVQ  DPTKNG+  GKI D GG  QAKQ  T+ TG G
Sbjct: 134 KHSDSESSGESSGHEDEKEKVKFEVQ--DPTKNGDPTGKIIDGGGDSQAKQAGTAPTGDG 193

Query: 187 QTSGG-GKKKKKKKKKKSSG----NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPS 246
           QT GG GKKKKKKKKKKS G    NA GE+ GD PANG  P  P PGH + GPQP  GP 
Sbjct: 194 QTGGGAGKKKKKKKKKKSGGGGGGNASGEAPGDPPANGAPPSEPNPGHSDTGPQPVAGPV 253

Query: 247 --PILANESRPRHFVTQYPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVH 306
             PI+ NES  RH+VTQY PHY  HP+YS+RY TAYA  SQP  ES+YATPQQYSYAYVH
Sbjct: 254 QVPIVVNESPTRHYVTQYQPHYIPHPVYSVRYSTAYAMSSQPRRESYYATPQQYSYAYVH 313

Query: 307 PGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV 331
           PGV+ E D PPSDYEPY SQPS SLEIFSDENPNGCS+V
Sbjct: 314 PGVRPEIDSPPSDYEPYSSQPSDSLEIFSDENPNGCSIV 346

BLAST of MC08g2239 vs. ExPASy TrEMBL
Match: A0A5A7U1B6 (Heavy metal-associated isoprenylated plant protein 36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00940 PE=4 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 4.40e-147
Identity = 258/339 (76.11%), Postives = 272/339 (80.24%), Query Frame = 0

Query: 7   SLSRTE-LEASVHLMAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLK 66
           SLSRTE L+ SV LMAE E KPEPKEVEE  EPPLIYKTWILKVSIHCEACKRKVKRVLK
Sbjct: 14  SLSRTEQLKPSVPLMAEQEPKPEPKEVEENLEPPLIYKTWILKVSIHCEACKRKVKRVLK 73

Query: 67  DVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKE 126
           D+EGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK    GK KGKSKKKE
Sbjct: 74  DIEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK----GKSKGKSKKKE 133

Query: 127 KQSDSESSG----NEDEKEKVKFEVQVTDPTKNGEAAGKINDDGG--QAKQPETSTTGGG 186
           K SDSESSG    +EDEKEKVKFEVQ  DPTKNG+  GKI D GG  QAKQ  T+ TG G
Sbjct: 134 KHSDSESSGESSGHEDEKEKVKFEVQ--DPTKNGDPTGKIIDGGGDSQAKQAGTAPTGDG 193

Query: 187 QTSGG-GKKKKKKKKKKSSG----NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPS 246
           QT GG GKKKKKKKKKKS G    NA GE+ GD PANG  P  P PGH + GPQP  GP 
Sbjct: 194 QTVGGAGKKKKKKKKKKSGGGGGGNASGEAPGDPPANGAPPSEPNPGHSDTGPQPMAGPV 253

Query: 247 --PILANESRPRHFVTQYPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVH 306
             PI+ NES  RH+VTQY PHY  HP+YS+RY TAYA  SQP  ES+YATPQQYSYAYVH
Sbjct: 254 QVPIVVNESPTRHYVTQYQPHYIPHPVYSVRYSTAYAMSSQPRRESYYATPQQYSYAYVH 313

Query: 307 PGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV 331
           PGV+ E D PPSDYEPY SQPS SLEIFSDENPNGCS+V
Sbjct: 314 PGVRPEIDSPPSDYEPYSSQPSDSLEIFSDENPNGCSIV 346

BLAST of MC08g2239 vs. ExPASy TrEMBL
Match: A0A5D3CPH0 (Heavy metal-associated isoprenylated plant protein 36 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00550 PE=4 SV=1)

HSP 1 Score: 426 bits (1095), Expect = 1.26e-146
Identity = 257/339 (75.81%), Postives = 271/339 (79.94%), Query Frame = 0

Query: 7   SLSRTE-LEASVHLMAEPEAKPEPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLK 66
           SLSRTE L+ SV LMAE E KPEPKEVEE  EPPLIYKTWILKVSIHCEACKRKVKRVLK
Sbjct: 14  SLSRTEQLKPSVPLMAEQEPKPEPKEVEENLEPPLIYKTWILKVSIHCEACKRKVKRVLK 73

Query: 67  DVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKE 126
           D+EGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK    GK KGKSKKKE
Sbjct: 74  DIEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEK----GKSKGKSKKKE 133

Query: 127 KQSDSESSG----NEDEKEKVKFEVQVTDPTKNGEAAGKINDDGG--QAKQPETSTTGGG 186
           K SDSESSG    +EDEKEKVKFEVQ  DPTKNG+  GKI D GG  QAKQ  T+ TG G
Sbjct: 134 KHSDSESSGESSGHEDEKEKVKFEVQ--DPTKNGDPTGKIIDGGGDSQAKQAGTAPTGDG 193

Query: 187 QTSGG-GKKKKKKKKKKSSG----NAGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPS 246
            T GG GKKKKKKKKKKS G    NA GE+ GD PANG  P  P PGH + GPQP  GP 
Sbjct: 194 HTGGGAGKKKKKKKKKKSGGGGGGNASGEAPGDPPANGAPPSEPNPGHSDTGPQPMAGPV 253

Query: 247 --PILANESRPRHFVTQYPPHYTTHPLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVH 306
             PI+ NES  RH+VTQY PHY  HP+YS+RY TAYA  SQP  ES+YATPQQYSYAYVH
Sbjct: 254 QVPIVVNESPTRHYVTQYQPHYIPHPVYSVRYSTAYAMSSQPRRESYYATPQQYSYAYVH 313

Query: 307 PGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV 331
           PGV+ E D PPSDYEPY SQPS SLEIFSDENPNGCS+V
Sbjct: 314 PGVRPEIDSPPSDYEPYSSQPSDSLEIFSDENPNGCSIV 346

BLAST of MC08g2239 vs. TAIR 10
Match: AT5G27690.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.2e-34
Identity = 134/352 (38.07%), Postives = 170/352 (48.30%), Query Frame = 0

Query: 28  EPKEVEEIAEPPLIYKTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGN 87
           E + V E    PL Y TW+L+VSIHCE CKRK+K++L  ++GVY T+ID+KQQKV V GN
Sbjct: 15  EQRHVFEDYPEPLRYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGN 74

Query: 88  VESETLIKKLLKTGKHAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQ 147
           VE E LIKK++K G+HAELWP   ++        +KK K+ + E+SG+ED+ E       
Sbjct: 75  VEPEILIKKIMKAGRHAELWPTSMENNINNDCNYQKKPKKDNEETSGDEDDDEN------ 134

Query: 148 VTDPTKNGEAAGKINDDGG-------QAKQPETSTTGGGQTSGGG---KKKKKKKKKKSS 207
                 N    G +   GG       Q KQ  T   G  Q  G G   KKKKKKKKKKS 
Sbjct: 135 ----NNNNNGGGDVGGGGGGGGGNFDQVKQVVTFVNGQLQPQGDGAPKKKKKKKKKKKSL 194

Query: 208 GNAGGESAGDAPANGGAPPGPAPGHPEPGPQPAP-------GPSPILAN----------- 267
           GN      G     GG  P    G PE     AP       GP P L +           
Sbjct: 195 GNTTVVMEGGGGGGGGGGPPQNDGPPETVIYSAPQDHHIIHGPPPHLHHHQQQNHPYPTV 254

Query: 268 ESRPRHFVTQY--------PPHY-----TTHPLYSLRYGTAYATCSQPHSE--SHYATP- 327
            S PRH   Q         PP +      T P Y++ Y T +   +   +E  S+Y  P 
Sbjct: 255 HSPPRHHPQQMYGPGPGLPPPSFYHTQPQTAPSYTVSYNTVHGPINNGGNETASYYTAPP 314

Query: 328 ----QQYSYAYVHPGVQTEFDPPPSDYEPYLSQPSGSLEIFSDENPNGCSVV 332
                 YSY YV  G    ++ PP ++  Y SQPS S E  S+ NPN C V+
Sbjct: 315 SHPTSYYSYEYVDTG----YESPPPEFYSYRSQPSESFESLSEGNPNSCCVM 352

BLAST of MC08g2239 vs. TAIR 10
Match: AT1G56210.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-25
Identity = 126/374 (33.69%), Postives = 169/374 (45.19%), Query Frame = 0

Query: 20  MAEPEAKPEPKEVEE----------------IAEPPLIYKTWILKVSIHCEACKRKVKRV 79
           MA  E K E K+ E                 I   PL YK+  LKVSIHCE CKRKVK++
Sbjct: 1   MATDEMKSETKKTEHKQKQSTQIKQDLPPPTIPPLPLPYKSCTLKVSIHCEGCKRKVKKI 60

Query: 80  LKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGKHAELWPEKSD----------- 139
           L  +EGV++ DID+KQ KV V G +  E L+KKL K GK+AE  PE  D           
Sbjct: 61  LTSIEGVFKVDIDVKQHKVTVIGIISPEILLKKLNKAGKNAEQLPEIPDPVDNKPKPVDP 120

Query: 140 -SKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTK----------NGEAAGKI 199
             K KKK K +K +  +++ SSG ++ ++    E    +  K           GE +G +
Sbjct: 121 KEKNKKKKKEEKVQITNEATSSGIDNPEKPGSGECDKPESEKPVDEKCLSGDGGETSGPV 180

Query: 200 ND---------DGGQAKQP-------------ETSTTGGGQTSGGGKKKKKKKKKKSSGN 259
            +         D G+ + P             +   TGG     G   KKKKKK +S   
Sbjct: 181 KEEKKDVLKEKDSGKEESPSPPADSSAPAAEKKAEDTGGAVPDNGKVGKKKKKKGQSLAT 240

Query: 260 AGGESAGDAPANGGAPPGPAPGHPEPGPQPAPGPSPILANESRPRHFVTQYPP--HYTTH 319
               + G A      PP  A  +  P  Q       I    + PRH +  YP   +Y   
Sbjct: 241 TNNPTDGPARTQ-SLPPPTATDYDRPINQ--INDHHITTTNNPPRHDLYPYPAQGYYAPQ 300

Query: 320 PLYSLRYGTAYATCSQPHSESHYATPQQYSYAYVHPGVQTEFDPPPSDYEPYLSQPSGSL 332
            +Y + Y  A    S   + S+Y  P  YSYAY+H G Q      PSD  P   +PS S 
Sbjct: 301 VMYGVSYNVAQPPVS-VDAASYYTPPPPYSYAYMHNGYQ------PSDQNPCQPRPSDSF 360

BLAST of MC08g2239 vs. TAIR 10
Match: AT5G19090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 5.3e-14
Identity = 56/143 (39.16%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 43  KTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGK 102
           +T +LKV+IHC+ CK+KVK++L+ +EGV+ T ID +  KV V GNV+   LIKKLLK+GK
Sbjct: 10  QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 103 HAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKNGEAAGKIN 162
           HAE+W       G  KG S   + Q +  +          +F+    D    G   G   
Sbjct: 70  HAEIW-------GAPKGGSNNNQNQPNLAN----------QFKAMQIDHGGKGGGGG--- 129

Query: 163 DDGGQAKQPETSTTGGGQTSGGG 186
             GG A   +    GGG   GGG
Sbjct: 130 -GGGPANNNKGQKIGGGGGGGGG 131

BLAST of MC08g2239 vs. TAIR 10
Match: AT5G19090.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 5.3e-14
Identity = 56/143 (39.16%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 43  KTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGK 102
           +T +LKV+IHC+ CK+KVK++L+ +EGV+ T ID +  KV V GNV+   LIKKLLK+GK
Sbjct: 10  QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 103 HAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKNGEAAGKIN 162
           HAE+W       G  KG S   + Q +  +          +F+    D    G   G   
Sbjct: 70  HAEIW-------GAPKGGSNNNQNQPNLAN----------QFKAMQIDHGGKGGGGG--- 129

Query: 163 DDGGQAKQPETSTTGGGQTSGGG 186
             GG A   +    GGG   GGG
Sbjct: 130 -GGGPANNNKGQKIGGGGGGGGG 131

BLAST of MC08g2239 vs. TAIR 10
Match: AT5G19090.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 5.3e-14
Identity = 56/143 (39.16%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 43  KTWILKVSIHCEACKRKVKRVLKDVEGVYETDIDLKQQKVVVKGNVESETLIKKLLKTGK 102
           +T +LKV+IHC+ CK+KVK++L+ +EGV+ T ID +  KV V GNV+   LIKKLLK+GK
Sbjct: 10  QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGK 69

Query: 103 HAELWPEKSDSKGKKKGKSKKKEKQSDSESSGNEDEKEKVKFEVQVTDPTKNGEAAGKIN 162
           HAE+W       G  KG S   + Q +  +          +F+    D    G   G   
Sbjct: 70  HAEIW-------GAPKGGSNNNQNQPNLAN----------QFKAMQIDHGGKGGGGG--- 129

Query: 163 DDGGQAKQPETSTTGGGQTSGGG 186
             GG A   +    GGG   GGG
Sbjct: 130 -GGGPANNNKGQKIGGGGGGGGG 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84J881.7e-3338.07Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX... [more]
Q9C7J61.5e-2433.69Heavy metal-associated isoprenylated plant protein 35 OS=Arabidopsis thaliana OX... [more]
F4JZL77.5e-1339.16Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX... [more]
Q9M8K51.8e-1135.57Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX... [more]
A2RVM81.1e-0831.33Heavy metal-associated isoprenylated plant protein 37 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_023537434.12.66e-15981.48heavy metal-associated isoprenylated plant protein 36 [Cucurbita pepo subsp. pep... [more]
XP_022937460.18.23e-15881.06heavy metal-associated isoprenylated plant protein 36 [Cucurbita moschata][more]
KAG6586249.12.73e-15680.75Heavy metal-associated isoprenylated plant protein 36, partial [Cucurbita argyro... [more]
XP_022965847.17.81e-15680.43heavy metal-associated isoprenylated plant protein 36 [Cucurbita maxima][more]
XP_038889488.11.60e-15576.83heavy metal-associated isoprenylated plant protein 36-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FGQ13.98e-15881.06heavy metal-associated isoprenylated plant protein 36 OS=Cucurbita moschata OX=3... [more]
A0A6J1HLE73.78e-15680.43heavy metal-associated isoprenylated plant protein 36 OS=Cucurbita maxima OX=366... [more]
A0A1S3BYY51.54e-14776.11uncharacterized protein LOC103494708 OS=Cucumis melo OX=3656 GN=LOC103494708 PE=... [more]
A0A5A7U1B64.40e-14776.11Heavy metal-associated isoprenylated plant protein 36 OS=Cucumis melo var. makuw... [more]
A0A5D3CPH01.26e-14675.81Heavy metal-associated isoprenylated plant protein 36 OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT5G27690.11.2e-3438.07Heavy metal transport/detoxification superfamily protein [more]
AT1G56210.11.0e-2533.69Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.15.3e-1439.16Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.25.3e-1439.16Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.35.3e-1439.16Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 42..106
e-value: 4.7E-18
score: 67.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 105..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 167..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..236
NoneNo IPR availablePANTHERPTHR45868:SF46HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 35coord: 20..331
NoneNo IPR availablePANTHERPTHR45868HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATEDcoord: 20..331
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 49..103
e-value: 1.3E-14
score: 54.3
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 50..106
score: 12.311873
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 47..105
e-value: 4.43537E-13
score: 61.4677
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 40..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2239.1MC08g2239.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding