MC08g2216 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2216
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionwall-associated receptor kinase-like 20
LocationMC08: 30951541 .. 30952158 (+)
RNA-Seq ExpressionMC08g2216
SyntenyMC08g2216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAATATCAATAAGACCATGCTTTTGATATTCGCTCTGTTTTTTCATGTATCTAAAGCATTAGTTTGTCATTCTTGTCCCAAATGTGGAAATCTTGAAGTTCCTTATCCGCTTAGCACAAATGACAACTGTGGAGACTCTAGGTACAGAATCTATTGCTATGACGGTATTCTTCAGTTCAAGTCAAGTGGTGGGTTTTACTATAATATCCATTCCATTGATCCCGATGCTTATAAGCTTATTATAAGGCCACCCCAAATAAAGAGAAAGAGTTGTTACTCTTCTGATCTCTCTTTAGGAGGTTTAAGGCTGGATGAACATTTGCCTTTCAACATTTCTACACAGAATACTGTCATGCTGCTGAATTGCTCAGATAATCTTATAAATTCACCCCTAAATTGTTCTACAAACAGCCTCTGTAGGCAGTTTGAGGAAAAAATGGAGGAATCAAGTGGGTGCAAGAACACCCTTTGCTGCACCTACTTGAAAGATTCAGCCATGACTTCCCACATGATAAGAGTCAGAATTGGAGGCTGCACTGCTTATACTGCTGTGGTCAATTTGAAGCCTGGAGATCCATTTGAAACCTGGAACTATGGAATTGAGTTGCAATGG

mRNA sequence

ATGAAGAATATCAATAAGACCATGCTTTTGATATTCGCTCTGTTTTTTCATGTATCTAAAGCATTAGTTTGTCATTCTTGTCCCAAATGTGGAAATCTTGAAGTTCCTTATCCGCTTAGCACAAATGACAACTGTGGAGACTCTAGGTACAGAATCTATTGCTATGACGGTATTCTTCAGTTCAAGTCAAGTGGTGGGTTTTACTATAATATCCATTCCATTGATCCCGATGCTTATAAGCTTATTATAAGGCCACCCCAAATAAAGAGAAAGAGTTGTTACTCTTCTGATCTCTCTTTAGGAGGTTTAAGGCTGGATGAACATTTGCCTTTCAACATTTCTACACAGAATACTGTCATGCTGCTGAATTGCTCAGATAATCTTATAAATTCACCCCTAAATTGTTCTACAAACAGCCTCTGTAGGCAGTTTGAGGAAAAAATGGAGGAATCAAGTGGGTGCAAGAACACCCTTTGCTGCACCTACTTGAAAGATTCAGCCATGACTTCCCACATGATAAGAGTCAGAATTGGAGGCTGCACTGCTTATACTGCTGTGGTCAATTTGAAGCCTGGAGATCCATTTGAAACCTGGAACTATGGAATTGAGTTGCAATGG

Coding sequence (CDS)

ATGAAGAATATCAATAAGACCATGCTTTTGATATTCGCTCTGTTTTTTCATGTATCTAAAGCATTAGTTTGTCATTCTTGTCCCAAATGTGGAAATCTTGAAGTTCCTTATCCGCTTAGCACAAATGACAACTGTGGAGACTCTAGGTACAGAATCTATTGCTATGACGGTATTCTTCAGTTCAAGTCAAGTGGTGGGTTTTACTATAATATCCATTCCATTGATCCCGATGCTTATAAGCTTATTATAAGGCCACCCCAAATAAAGAGAAAGAGTTGTTACTCTTCTGATCTCTCTTTAGGAGGTTTAAGGCTGGATGAACATTTGCCTTTCAACATTTCTACACAGAATACTGTCATGCTGCTGAATTGCTCAGATAATCTTATAAATTCACCCCTAAATTGTTCTACAAACAGCCTCTGTAGGCAGTTTGAGGAAAAAATGGAGGAATCAAGTGGGTGCAAGAACACCCTTTGCTGCACCTACTTGAAAGATTCAGCCATGACTTCCCACATGATAAGAGTCAGAATTGGAGGCTGCACTGCTTATACTGCTGTGGTCAATTTGAAGCCTGGAGATCCATTTGAAACCTGGAACTATGGAATTGAGTTGCAATGG

Protein sequence

MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVVNLKPGDPFETWNYGIELQW
Homology
BLAST of MC08g2216 vs. ExPASy Swiss-Prot
Match: Q9LZM4 (Wall-associated receptor kinase-like 20 OS=Arabidopsis thaliana OX=3702 GN=WAKL20 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.4e-38
Identity = 82/186 (44.09%), Postives = 112/186 (60.22%), Query Frame = 0

Query: 27  CPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGFYYNIHSIDPDAYKLIIRPP 86
           CP CG + VPYPLST   CGD  YRI C  G L F +  G  Y I SI+    ++++RPP
Sbjct: 43  CPNCGPMVVPYPLSTGPTCGDQAYRINCVGGKLYFGALHGSSYVITSINSVTQRIVLRPP 102

Query: 87  QIKRK-SCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDNLINSPLNCSTNSLCRQFE 146
            +    SC S+D+S  GL LD HLPF+I++ NT++LLNCS  ++ +P++CS  SLC  + 
Sbjct: 103 GLASSVSCISADVSKQGLELDPHLPFSITSSNTILLLNCSQAMLQAPIDCSPTSLCYSY- 162

Query: 147 EKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVVNLKPG----DPFETW-NY 206
            K   S   K  LCCT+  D + T++ IR+  GGC AY + V L P      P + W + 
Sbjct: 163 IKNNASPCSKAPLCCTFRTDGSQTAYTIRINGGGCLAYQSFVGLNPNKEVPPPGKKWPDT 222

BLAST of MC08g2216 vs. ExPASy Swiss-Prot
Match: Q9M342 (Wall-associated receptor kinase-like 15 OS=Arabidopsis thaliana OX=3702 GN=WAKL15 PE=2 SV=2)

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-30
Identity = 74/212 (34.91%), Postives = 112/212 (52.83%), Query Frame = 0

Query: 4   INKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYD-GILQFK 63
           ++ T   +  L +  S       CP CG+  VPYPLST  +CGD  YRI C + G L F 
Sbjct: 6   LSLTTFTLSLLIYFSSTTQAFKRCPNCGSTRVPYPLSTGLDCGDPGYRIRCDNYGSLWFD 65

Query: 64  SSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLL 123
           +  G    I +IDP   + ++RPP  ++  C S D+   G++LD +LPFN+S  NTV+++
Sbjct: 66  TLNGSTNPIKTIDPSGQRFVLRPPGFEQNKCVSVDIKYHGIQLDLNLPFNVSCSNTVIIM 125

Query: 124 NCS----DNLINSPLNCSTNSLCRQF-EEKMEESSGCKN-TLCCTYLKDSAMTSHMI-RV 183
           NC+    D   +   NCS NSLC +F    +E    C+  T CC Y   +++ ++ + R 
Sbjct: 126 NCTKDGLDAYSSQGFNCSDNSLCHKFLNANLEARGNCRGVTSCCWYKTGASVNTYKVYRA 185

Query: 184 RIGGCTAYTAVVNLKPGDPFETWNY-GIELQW 207
           R   C+AY + +NL    P   W    +E+ W
Sbjct: 186 RPDMCSAYQSFMNLDLTIPVSKWGEPAVEILW 217

BLAST of MC08g2216 vs. NCBI nr
Match: XP_022156422.1 (wall-associated receptor kinase-like 20 [Momordica charantia])

HSP 1 Score: 434 bits (1116), Expect = 9.00e-154
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0

Query: 1   MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ 60
           MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ
Sbjct: 1   MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ 60

Query: 61  FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM 120
           FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM
Sbjct: 61  FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM 120

Query: 121 LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC 180
           LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC
Sbjct: 121 LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC 180

Query: 181 TAYTAVVNLKPGDPFETWNYGIELQW 206
           TAYTAVVNLKPGDPFETWNYGIELQW
Sbjct: 181 TAYTAVVNLKPGDPFETWNYGIELQW 206

BLAST of MC08g2216 vs. NCBI nr
Match: KAA0048047.1 (wall-associated receptor kinase-like 20 [Cucumis melo var. makuwa])

HSP 1 Score: 346 bits (887), Expect = 5.72e-119
Identity = 162/199 (81.41%), Postives = 179/199 (89.95%), Query Frame = 0

Query: 8   MLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGF 67
           M  IFALFF +SKA+   +C KCGNLEVPYPLSTNDNCGDSRY++YC   ILQFKSS GF
Sbjct: 1   MFFIFALFFQLSKAIAFSACSKCGNLEVPYPLSTNDNCGDSRYKVYCNGDILQFKSSEGF 60

Query: 68  YYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDN 127
           YYNIHSIDP+AYKLII PP+I++ SC+SSDLSLGGLRLDE+LPFN+ST NTVMLLNCS+N
Sbjct: 61  YYNIHSIDPNAYKLIISPPEIQKGSCFSSDLSLGGLRLDENLPFNVSTHNTVMLLNCSNN 120

Query: 128 LINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVV 187
           LINSPLNCS NS CRQFEEK++ESSGCKNTLCCTYLKDSAMT+HMIRVRIGGCTAYT+VV
Sbjct: 121 LINSPLNCSINSPCRQFEEKIKESSGCKNTLCCTYLKDSAMTAHMIRVRIGGCTAYTSVV 180

Query: 188 NLKPGDPFETWNYGIELQW 206
           N KPGD  ETW YGIELQW
Sbjct: 181 NFKPGDSVETWKYGIELQW 199

BLAST of MC08g2216 vs. NCBI nr
Match: TYJ96489.1 (wall-associated receptor kinase-like 20 [Cucumis melo var. makuwa])

HSP 1 Score: 343 bits (881), Expect = 4.70e-118
Identity = 161/199 (80.90%), Postives = 178/199 (89.45%), Query Frame = 0

Query: 8   MLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGF 67
           M  IFALFF +SKA+   +C KCGNLEVPYPLSTNDNCGDSRY++YC   ILQFKSS GF
Sbjct: 1   MFFIFALFFQLSKAIAFSACSKCGNLEVPYPLSTNDNCGDSRYKVYCNGDILQFKSSEGF 60

Query: 68  YYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDN 127
           YYNIHSIDP+AYKLII PP+I++ SC+SSDLSLGGLRLDE+LPFN+ST  TVMLLNCS+N
Sbjct: 61  YYNIHSIDPNAYKLIISPPEIQKGSCFSSDLSLGGLRLDENLPFNVSTHKTVMLLNCSNN 120

Query: 128 LINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVV 187
           LINSPLNCS NS CRQFEEK++ESSGCKNTLCCTYLKDSAMT+HMIRVRIGGCTAYT+VV
Sbjct: 121 LINSPLNCSINSPCRQFEEKIKESSGCKNTLCCTYLKDSAMTAHMIRVRIGGCTAYTSVV 180

Query: 188 NLKPGDPFETWNYGIELQW 206
           N KPGD  ETW YGIELQW
Sbjct: 181 NFKPGDSVETWKYGIELQW 199

BLAST of MC08g2216 vs. NCBI nr
Match: KAG6599200.1 (Wall-associated receptor kinase-like 20, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030135.1 Wall-associated receptor kinase-like 20, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 335 bits (860), Expect = 9.85e-115
Identity = 159/207 (76.81%), Postives = 176/207 (85.02%), Query Frame = 0

Query: 1   MKNINKTMLLIFALFFHVSKALVCH-SCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGIL 60
           MKNI  T+  I  LFF  SKA +   +CPKCG+LEVP+PLSTN NCGDSRYR+YC+ G+L
Sbjct: 1   MKNIMNTLFFILPLFFQASKAAIASPACPKCGDLEVPFPLSTNANCGDSRYRVYCHHGVL 60

Query: 61  QFKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTV 120
           QFKSSGGFYYNIHSIDP+A KLII PPQI + SC SSDL LGGLRLDE LPFNIST NTV
Sbjct: 61  QFKSSGGFYYNIHSIDPNASKLIISPPQINKGSCSSSDLYLGGLRLDESLPFNISTHNTV 120

Query: 121 MLLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGG 180
           MLLNCSDNL+ SPLNCS NS CRQFEEK EE +GC+NTLCC+YLKDSAMT+HMIRVR+GG
Sbjct: 121 MLLNCSDNLVFSPLNCSVNSPCRQFEEKTEEGNGCRNTLCCSYLKDSAMTAHMIRVRVGG 180

Query: 181 CTAYTAVVNLKPGDPFETWNYGIELQW 206
           CTAYT+VVN K GD  ETWNYGIELQW
Sbjct: 181 CTAYTSVVNFKAGDSIETWNYGIELQW 207

BLAST of MC08g2216 vs. NCBI nr
Match: XP_022999379.1 (wall-associated receptor kinase-like 20 [Cucurbita maxima])

HSP 1 Score: 334 bits (856), Expect = 4.01e-114
Identity = 160/207 (77.29%), Postives = 176/207 (85.02%), Query Frame = 0

Query: 1   MKNINKTMLLIFALFFHVSKALVCH-SCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGIL 60
           MKNI  TM  IF LFF  SKA +   +CPKCG+LEVP+PLSTN NCGDSRY++YC  GIL
Sbjct: 1   MKNIMNTMFFIFPLFFEASKAAIAFPACPKCGDLEVPFPLSTNGNCGDSRYKVYCRHGIL 60

Query: 61  QFKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTV 120
           QFKSSGGFYYN+HSID +A KLII PPQI + SC SSDL LGGLRLDE LPFNIST NTV
Sbjct: 61  QFKSSGGFYYNVHSIDANASKLIISPPQINKGSCSSSDLYLGGLRLDESLPFNISTHNTV 120

Query: 121 MLLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGG 180
           MLLNCSDNL+ SPLNCS NS CRQFEEK EE++GCK+TLCCTYLKDSAMT+HMIRVR+GG
Sbjct: 121 MLLNCSDNLVFSPLNCSLNSPCRQFEEKTEEANGCKDTLCCTYLKDSAMTAHMIRVRVGG 180

Query: 181 CTAYTAVVNLKPGDPFETWNYGIELQW 206
           CTAYT+VVN K GD  ETWNYGIELQW
Sbjct: 181 CTAYTSVVNFKGGDSIETWNYGIELQW 207

BLAST of MC08g2216 vs. ExPASy TrEMBL
Match: A0A6J1DS11 (wall-associated receptor kinase-like 20 OS=Momordica charantia OX=3673 GN=LOC111023315 PE=4 SV=1)

HSP 1 Score: 434 bits (1116), Expect = 4.36e-154
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0

Query: 1   MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ 60
           MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ
Sbjct: 1   MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ 60

Query: 61  FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM 120
           FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM
Sbjct: 61  FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM 120

Query: 121 LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC 180
           LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC
Sbjct: 121 LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC 180

Query: 181 TAYTAVVNLKPGDPFETWNYGIELQW 206
           TAYTAVVNLKPGDPFETWNYGIELQW
Sbjct: 181 TAYTAVVNLKPGDPFETWNYGIELQW 206

BLAST of MC08g2216 vs. ExPASy TrEMBL
Match: A0A0A0LGH4 (GUB_WAK_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G033280 PE=4 SV=1)

HSP 1 Score: 358 bits (919), Expect = 4.71e-124
Identity = 168/206 (81.55%), Postives = 185/206 (89.81%), Query Frame = 0

Query: 1   MKNINKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQ 60
           MKNI KTM +IFALFF +SKA+   +C KCGNLEVPYPLSTNDNCGDSRY+++C D ILQ
Sbjct: 1   MKNIKKTMFIIFALFFQLSKAIAFSACSKCGNLEVPYPLSTNDNCGDSRYKVHCNDDILQ 60

Query: 61  FKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVM 120
           FKSS GFYYNIHSIDP+AYKLII PP+I+   C+SSDLSLGGLRLDE+LPFN+ST NTVM
Sbjct: 61  FKSSEGFYYNIHSIDPNAYKLIISPPEIQNGRCFSSDLSLGGLRLDENLPFNVSTHNTVM 120

Query: 121 LLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGC 180
           LLNCS+NLINSPLNCS NS CRQFEEKM E+SGCKNTLCCTYLKDSAMT+HMIRVRIGGC
Sbjct: 121 LLNCSNNLINSPLNCSINSPCRQFEEKMIEASGCKNTLCCTYLKDSAMTAHMIRVRIGGC 180

Query: 181 TAYTAVVNLKPGDPFETWNYGIELQW 206
           TAYT+VVN KPGDP ETW YGIELQW
Sbjct: 181 TAYTSVVNFKPGDPVETWKYGIELQW 206

BLAST of MC08g2216 vs. ExPASy TrEMBL
Match: A0A5A7U1E3 (Wall-associated receptor kinase-like 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001150 PE=4 SV=1)

HSP 1 Score: 346 bits (887), Expect = 2.77e-119
Identity = 162/199 (81.41%), Postives = 179/199 (89.95%), Query Frame = 0

Query: 8   MLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGF 67
           M  IFALFF +SKA+   +C KCGNLEVPYPLSTNDNCGDSRY++YC   ILQFKSS GF
Sbjct: 1   MFFIFALFFQLSKAIAFSACSKCGNLEVPYPLSTNDNCGDSRYKVYCNGDILQFKSSEGF 60

Query: 68  YYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDN 127
           YYNIHSIDP+AYKLII PP+I++ SC+SSDLSLGGLRLDE+LPFN+ST NTVMLLNCS+N
Sbjct: 61  YYNIHSIDPNAYKLIISPPEIQKGSCFSSDLSLGGLRLDENLPFNVSTHNTVMLLNCSNN 120

Query: 128 LINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVV 187
           LINSPLNCS NS CRQFEEK++ESSGCKNTLCCTYLKDSAMT+HMIRVRIGGCTAYT+VV
Sbjct: 121 LINSPLNCSINSPCRQFEEKIKESSGCKNTLCCTYLKDSAMTAHMIRVRIGGCTAYTSVV 180

Query: 188 NLKPGDPFETWNYGIELQW 206
           N KPGD  ETW YGIELQW
Sbjct: 181 NFKPGDSVETWKYGIELQW 199

BLAST of MC08g2216 vs. ExPASy TrEMBL
Match: A0A5D3BBU4 (Wall-associated receptor kinase-like 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001350 PE=4 SV=1)

HSP 1 Score: 343 bits (881), Expect = 2.27e-118
Identity = 161/199 (80.90%), Postives = 178/199 (89.45%), Query Frame = 0

Query: 8   MLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGF 67
           M  IFALFF +SKA+   +C KCGNLEVPYPLSTNDNCGDSRY++YC   ILQFKSS GF
Sbjct: 1   MFFIFALFFQLSKAIAFSACSKCGNLEVPYPLSTNDNCGDSRYKVYCNGDILQFKSSEGF 60

Query: 68  YYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDN 127
           YYNIHSIDP+AYKLII PP+I++ SC+SSDLSLGGLRLDE+LPFN+ST  TVMLLNCS+N
Sbjct: 61  YYNIHSIDPNAYKLIISPPEIQKGSCFSSDLSLGGLRLDENLPFNVSTHKTVMLLNCSNN 120

Query: 128 LINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVV 187
           LINSPLNCS NS CRQFEEK++ESSGCKNTLCCTYLKDSAMT+HMIRVRIGGCTAYT+VV
Sbjct: 121 LINSPLNCSINSPCRQFEEKIKESSGCKNTLCCTYLKDSAMTAHMIRVRIGGCTAYTSVV 180

Query: 188 NLKPGDPFETWNYGIELQW 206
           N KPGD  ETW YGIELQW
Sbjct: 181 NFKPGDSVETWKYGIELQW 199

BLAST of MC08g2216 vs. ExPASy TrEMBL
Match: A0A6J1KGX8 (wall-associated receptor kinase-like 20 OS=Cucurbita maxima OX=3661 GN=LOC111493768 PE=4 SV=1)

HSP 1 Score: 334 bits (856), Expect = 1.94e-114
Identity = 160/207 (77.29%), Postives = 176/207 (85.02%), Query Frame = 0

Query: 1   MKNINKTMLLIFALFFHVSKALVCH-SCPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGIL 60
           MKNI  TM  IF LFF  SKA +   +CPKCG+LEVP+PLSTN NCGDSRY++YC  GIL
Sbjct: 1   MKNIMNTMFFIFPLFFEASKAAIAFPACPKCGDLEVPFPLSTNGNCGDSRYKVYCRHGIL 60

Query: 61  QFKSSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTV 120
           QFKSSGGFYYN+HSID +A KLII PPQI + SC SSDL LGGLRLDE LPFNIST NTV
Sbjct: 61  QFKSSGGFYYNVHSIDANASKLIISPPQINKGSCSSSDLYLGGLRLDESLPFNISTHNTV 120

Query: 121 MLLNCSDNLINSPLNCSTNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGG 180
           MLLNCSDNL+ SPLNCS NS CRQFEEK EE++GCK+TLCCTYLKDSAMT+HMIRVR+GG
Sbjct: 121 MLLNCSDNLVFSPLNCSLNSPCRQFEEKTEEANGCKDTLCCTYLKDSAMTAHMIRVRVGG 180

Query: 181 CTAYTAVVNLKPGDPFETWNYGIELQW 206
           CTAYT+VVN K GD  ETWNYGIELQW
Sbjct: 181 CTAYTSVVNFKGGDSIETWNYGIELQW 207

BLAST of MC08g2216 vs. TAIR 10
Match: AT5G02070.1 (Protein kinase family protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.0e-39
Identity = 82/186 (44.09%), Postives = 112/186 (60.22%), Query Frame = 0

Query: 27  CPKCGNLEVPYPLSTNDNCGDSRYRIYCYDGILQFKSSGGFYYNIHSIDPDAYKLIIRPP 86
           CP CG + VPYPLST   CGD  YRI C  G L F +  G  Y I SI+    ++++RPP
Sbjct: 43  CPNCGPMVVPYPLSTGPTCGDQAYRINCVGGKLYFGALHGSSYVITSINSVTQRIVLRPP 102

Query: 87  QIKRK-SCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDNLINSPLNCSTNSLCRQFE 146
            +    SC S+D+S  GL LD HLPF+I++ NT++LLNCS  ++ +P++CS  SLC  + 
Sbjct: 103 GLASSVSCISADVSKQGLELDPHLPFSITSSNTILLLNCSQAMLQAPIDCSPTSLCYSY- 162

Query: 147 EKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGGCTAYTAVVNLKPG----DPFETW-NY 206
            K   S   K  LCCT+  D + T++ IR+  GGC AY + V L P      P + W + 
Sbjct: 163 IKNNASPCSKAPLCCTFRTDGSQTAYTIRINGGGCLAYQSFVGLNPNKEVPPPGKKWPDT 222

BLAST of MC08g2216 vs. TAIR 10
Match: AT3G53840.1 (Protein kinase superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.0e-31
Identity = 74/212 (34.91%), Postives = 112/212 (52.83%), Query Frame = 0

Query: 4   INKTMLLIFALFFHVSKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYRIYCYD-GILQFK 63
           ++ T   +  L +  S       CP CG+  VPYPLST  +CGD  YRI C + G L F 
Sbjct: 6   LSLTTFTLSLLIYFSSTTQAFKRCPNCGSTRVPYPLSTGLDCGDPGYRIRCDNYGSLWFD 65

Query: 64  SSGGFYYNIHSIDPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLL 123
           +  G    I +IDP   + ++RPP  ++  C S D+   G++LD +LPFN+S  NTV+++
Sbjct: 66  TLNGSTNPIKTIDPSGQRFVLRPPGFEQNKCVSVDIKYHGIQLDLNLPFNVSCSNTVIIM 125

Query: 124 NCS----DNLINSPLNCSTNSLCRQF-EEKMEESSGCKN-TLCCTYLKDSAMTSHMI-RV 183
           NC+    D   +   NCS NSLC +F    +E    C+  T CC Y   +++ ++ + R 
Sbjct: 126 NCTKDGLDAYSSQGFNCSDNSLCHKFLNANLEARGNCRGVTSCCWYKTGASVNTYKVYRA 185

Query: 184 RIGGCTAYTAVVNLKPGDPFETWNY-GIELQW 207
           R   C+AY + +NL    P   W    +E+ W
Sbjct: 186 RPDMCSAYQSFMNLDLTIPVSKWGEPAVEILW 217

BLAST of MC08g2216 vs. TAIR 10
Match: AT1G11915.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: root; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G17350.1); Has 261 Blast hits to 261 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 261; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.0 bits (136), Expect = 2.1e-08
Identity = 54/213 (25.35%), Postives = 87/213 (40.85%), Query Frame = 0

Query: 19  SKALVCHSCPKCGNLEVPYPLSTNDNCGDSRYR--IYCYDG--ILQFKSSGGFYYNIHSI 78
           S++ +C S   CGN+ + YP S +D CG   YR  + C D    L+ ++  G  Y + SI
Sbjct: 26  SQSNLCRS--SCGNIPINYPFSIDDGCGSPYYRHMLICSDNDTKLELRTPSG-KYPVKSI 85

Query: 79  DPDAYKLIIRPPQIKRKSCYSSDLSLGGLRLDEHLPFNISTQNTVMLLNCSDNLI---NS 138
                 L++  P +       +        +D    F +S QN  +  NC+ + +     
Sbjct: 86  SYSDPHLLVSDPFMWNCQDRDNFRPTRSFSIDSSTHFTVSPQNDYLFFNCNTDKVIVEPK 145

Query: 139 PLNC------------STNSLCRQFEEKMEESSGCKNTLCCTYLKDSAMTSHMIRVRIGG 198
           PL C            S++ LCR   E    S+      CC+Y   +  +   +R+ +  
Sbjct: 146 PLFCERFPDRCDSSCDSSSYLCRHLPEC--GSALGSRVSCCSYYPKATQS---LRLMLQD 205

Query: 199 CTAYTAV------VNLKPGDPFETWNYGIELQW 207
           C  YT+V      V   P D F    YGI + +
Sbjct: 206 CATYTSVYWRSTGVENAPYDQFP--EYGIRVDY 228

BLAST of MC08g2216 vs. TAIR 10
Match: AT4G03230.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 48.1 bits (113), Expect = 9.6e-06
Identity = 26/61 (42.62%), Postives = 33/61 (54.10%), Query Frame = 0

Query: 27  CPKCGNLEVPYPLSTNDNCGDSRYRIY---CYDGILQFKSSGGFYYNIHSIDPDAYKLII 85
           C  CG   +PYPLST   CGDS Y  +      G + FK S    YNI SI+PD  + +I
Sbjct: 436 CVTCGTNIIPYPLSTAPGCGDSNYLSFNCNMSTGQVIFKGSNS-SYNITSINPDTRRFLI 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZM41.4e-3844.09Wall-associated receptor kinase-like 20 OS=Arabidopsis thaliana OX=3702 GN=WAKL2... [more]
Q9M3421.4e-3034.91Wall-associated receptor kinase-like 15 OS=Arabidopsis thaliana OX=3702 GN=WAKL1... [more]
Match NameE-valueIdentityDescription
XP_022156422.19.00e-154100.00wall-associated receptor kinase-like 20 [Momordica charantia][more]
KAA0048047.15.72e-11981.41wall-associated receptor kinase-like 20 [Cucumis melo var. makuwa][more]
TYJ96489.14.70e-11880.90wall-associated receptor kinase-like 20 [Cucumis melo var. makuwa][more]
KAG6599200.19.85e-11576.81Wall-associated receptor kinase-like 20, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022999379.14.01e-11477.29wall-associated receptor kinase-like 20 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DS114.36e-154100.00wall-associated receptor kinase-like 20 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0LGH44.71e-12481.55GUB_WAK_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G03328... [more]
A0A5A7U1E32.77e-11981.41Wall-associated receptor kinase-like 20 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A5D3BBU42.27e-11880.90Wall-associated receptor kinase-like 20 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1KGX81.94e-11477.29wall-associated receptor kinase-like 20 OS=Cucurbita maxima OX=3661 GN=LOC111493... [more]
Match NameE-valueIdentityDescription
AT5G02070.11.0e-3944.09Protein kinase family protein [more]
AT3G53840.11.0e-3134.91Protein kinase superfamily protein [more]
AT1G11915.12.1e-0825.35unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT4G03230.19.6e-0642.62S-locus lectin protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 24..81
e-value: 2.5E-7
score: 31.1
NoneNo IPR availablePANTHERPTHR33355:SF14WALL-ASSOCIATED RECEPTOR KINASE-LIKE PROTEINcoord: 8..206
NoneNo IPR availablePANTHERPTHR33355WALL-ASSOCIATED RECEPTOR KINASE CARBOXY-TERMINAL PROTEIN-RELATEDcoord: 8..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2216.1MC08g2216.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0030247 polysaccharide binding