MC08g2204 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2204
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionH(+)-exporting diphosphatase
LocationMC08: 30864579 .. 30869557 (+)
RNA-Seq ExpressionMC08g2204
SyntenyMC08g2204
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCAGACTGCTGCACATCAAAACCCGAATTTGCTGTTGTTATATATGCCAGGAAACCTCAGGGAACATTGTGATCTTTGAAAATGGGTGGTTTGAGTGAGGGTCTGACACAGTTGCTGATACCTGCAGCTGCCTTGCTTGGTATTGGGTTTGCCCTTCTTCAATGGCTTTTGGTCTCAAGAGTTAAGATTTCTGACTACTCTGATATGGATGAAAGCCAAGAAAATAACTTGATTGAAGGAGGAGGAGAGGAAGGTATTGACGATTTTGAGGTAGTCCTAAAGTGTGCTGAAATTCAGAAGGCCATTTCTGTTGGTGAGCTTTTTTTTTCTTCTGTTGAAAATTTATTGCTTTTCCTTTTCTTTTCTTCTAAAATACATTTTTGACAATTACTTGATTAGGATCTCTGCTCTCAACTTTTAAAAGCTTTATGCATCTCATTTGGAGAAATTCATTTTTTGTTTGGATATCAACTCTCAAGAAATGATAAATTTGTTCTAGGACAGGACATCCTTGTCTTCCAAACAAACATTGAAGAATAATCTCTTTCATTGCCATTTTTAAAGATGAAAAAACACAAAAAGGTGAATTTGAAAGGATGCAAAAGTACTCTCTCCCTTACCCCACGGAAAAAATATAGCATTATCAAGTGATAATCAAGGTAGAAATGACATAATTTTTGCTCCTATGACATTGTAGAGTTGAATCCCCTATGTCATAATATGTCCTTGAGTTTATATCCGAAAATATCTTCGATTTGGTGCAATGCACAATATGCTGCAGAAAGATTGGATAGAAGATTGTTGCAGTGTATAAGATGAACTAAAAATTTCATCCTTGCGAAGCATTATAAAAAGTATAGTAGTATATTGATCTGAATTATTTGTTTTTTTGCTTTCAGGGGCAACATCATTTTTATTCACCCAGTATAAGTACCTAGTTGTCTTCATGGTAGCATTTGGAGCCATCATTTTCCTGTTCCTTGGCTCAGTTAAGAGCTTCAGCACCAAAAGTGAACCTTGCACATACAAGAAGGGAAATATGTGTAAACCAGCTCTAGCCAATGCATTCTTTAGCACTATTGCCTTTTTGCTAGGTGGTCTTACCTCGGTTCTTTCTGGTTTCCTTGGAATGAAGATTGCAACTTATGCAAATGCCCGAACGAGTCTAGAAGCACGAAAAGGCATTGGAAAAGCATTTGTTATCGCTTTTCGCTCGGGTGCTGTGATGGGATTCCTTCTAGCTGCCAATGGCCTCCTTGTATTATATGTCTCCATCAATTTGTTTAAGCTGTATTACGGTGACGACTGGGAAGGACTGTATGAATCTATCACGGGTTATGGTCTTGGAGGTTCTTCTATGGCTCTATTTGGAAGAGTGGGGGGAGGCATATACACAAAAGCAGCTGATGTTGGAGCTGATCTTGTAGGGAAAATTGAAAAGAATATCCCTGAAGACGATCCACGAAACCCTGCTGTAATTTTCTATACATTTCTTTTGTCACTCTAATTTCTATGGTACTTTTCAATACTTGAGGAATTAAAGAGAGATTATTCACTCGTATTCGACTCTATGAGATTATCGAGGCGTTCAGTCTGATGATATATAATTTGTTTTCCAGGTTATAGCTGACAATGTGGGTGACAATGTAGGAGACATAGCAGGAATGGGCTCTGACTTATTTGGATCTTATGCTGAATCAACCTGTGCAGCACTTTTTGTAGCATCAATATCATCCTTCGGCTTCACACATAACTATGCAGCCATGTTGTACCCTCTACTTATAAGCTCAATGGGCATTGTGATTTGCTTAATAACTACACTTTTCGCAACCGATCTCATTGAGATTAAGAAAGTGAGTGGAGTTGAACCATCATTAAAGCGACAGCTTCTTTTCTCCACTGTCCTGATGACAGCAGGGGTAGCTTTGGTCAGCTTGGTTGCTTTACCTTCGAAATTCACTCTCTACGATTTCGGCAACGACAAGGTCGTAAAGAACTGGTACGACTTTGCTGATTACAAGAATCTCTTTTCCCCCGTAAACATTAGTCTTTATGCTGACTAAAATTATATCCAAATGCAGGCACCTTTTCTTCTGTGTTGTGACAGGATTATGGGCAGGACTTGTGATTGGATATACAACTGAATACTACACTAGTAATGCTTATAGGTAAAATATCTCTTGGAATAAATATATTCTAACCTATCGGCGGAAGCTTTTGAGTTTTCTAGTAGTTTTAATATGATATCGAAGCAGGAGGCTTTGAGTTGAACTCGTATAACGTTATGGGAGTGTAAATGTTAAGAATACATATAAAAGAATAACTTTATCCTAATCTAATTTAAACATTTGGCATTTGGGTTTAGTGGTTTAACAATATCTTTCTTGAAATTGAAAAAGGCATTCGTATAAAATCTGATATATGGCATGTCTCATTTGGCTGAAACATGAATTGTGATGAGATCATTTGTGCAGTCCGGTGCAGGATGTGGCTGATTCCTGTAGAACCGGTGCCGCAACAAATGTGATATTTGGATTAGCTCTTGGTTACAAATCCGTCATAATTCCCATATTTTCGATCGCCTTATCCATTTACGTGAGCTTCCGCTTGGCAGCCATGTATGGAATTGCGATGGCTGCTTTAGGAATGCTTAGCACCATTGCCACTGGTCTAGCCATTGATGCATATGGACCCATTAGTGACAATGCAGGTGGAATAGCCGAAATGGCTGGAATGAGCCACGAGGTACGCGAGAGAACCGACGCTTTAGACGCTGCAGGAAATACAACTGCTGCCATAGGCAAGGTGCCACTTCTTCAACAATCATGTTTCTGTTCTAAACATTCTGAGAGCTAGCTGCATCATGTTGCTCGCAACTTGCTGTTCTTATATTGTGCAGGGCTTTGCCATTGGATCTGCTGCTCTTGTTTCCCTCGCCTTGTTCGGTGCCTTTGTGAGCCGAGCAGGCATCGAAACAGTCGACGTCATGGACCCGAAAGTTTTCATTGGACTGATAGTAGGTGCCATGCTTCCATATTGGTTCTCAGCAATGACAATGAAGAGTGTTGGCAGTGCAGCGCTGAAGATGGTCGAGGAAGTTCGACGACAATTCAACTCGATCCCGGGGCTGATGGAAGGCAGGACAAAGCCGGACTACGCGAATTGTGTGAAGATATCGACGGATGCTTCACTTAGAGAGATGATCCCACCTGGAGCTCTGGTTTTGGTAACACCACTTATTGCTGGAACATTCTTTGGAGTTGAAACTCTTGCTGGGCTGCTGGCTGGTTCCCTTGTTTCTGGTGTGCAGGTATGTCAATCTAACTGTCTCCCCACATTTTAAACAAATTATTCTGTTACAAAACTCCGGAGAATTTCATATTACTTTAGGCTTGTTTCATGACGATATCGATATTCTTTTCAGTTTTCTGTTTTTAAAATTTGTGTTGGCTTCCGACACCATCGTTTTTTCACATTTTTTTTCCTTTTACATGTTTGAATTGACAATTTTTAAGAACATAAATCAGTTTTTTTTTTCAATTTTTCGCTTAGATAACAAAACAAATAAATACAGAGGTAGAAGTAGTGGTTTTAATCTTAAAAAACAAGATAGGAAAACATTAATGAAAAAAACAATAAAAAATAAACAAGAAATGAAAAAGAAAAATGAAAAAAAGTTGGCCTTTAGTTTTTCTATCCTCATTTCTCATAGGTCCCCTCCACTTCTTTCTTTCCTTTTACTACTACAAGGGTATGGACTCCTCTTTTTTCGACAAGGACATATTTATCGTTGACTTCTTGCTCTTTTTCCCGATCCTTAACTTTTAACAAGATTGATACACGTGGAATTGATGCATAGAAAGTGGTAGTAATATTTTCCCATGAACAATACTTAGATGCTTATTGATCAGCACCCACCAATCAAAATTTGATGGCTCCGAAAAATAGAAAAAAAATATCTCTTTTTCTACATTTCTTTCTTCTACTTATTAAAAACTTAACGGTGGTCGGTAAAATATGTGGGTGCTAATAGGTAAGTACTTAAGTATTGTTATTTTCTCTCTCCTCCCGGTTTCTTTGCCAGCTTTTCTTCTTCTTCATTGCTTTTGTCATGCTTTAACCTTGCTTGAAACACAACCATTGTAAGTGGCAATGATGATGAGCTTTCTTTACCATAACTTTACTTCTTCAAATAAATGGCTCTAAGTTGTCTCTCTTCAAACTTCTTAGGTTGCTATCTCTGCTTCAAACACAGGTGGGGCTTGGGATAATGCCAAAAAGTACATAGAGGTAACATCTCCTCCACACTCTTTTTAGCATTCTTTTTCTCAGTTCGAATGTGTTCGGGAGTGTTTTTTCAATATCTCGTAGTGCTTTTGACTCGCTTTTTAAATCTTTCGTAGCTCTTGGACTATGTATGAGAAAAACGCTTTTTAAAACTTCTAAAATACTGACAGTACTTCAACATAGTGATATATTATTGTTTTGTAAGAACTATGATTGGGTGGATCAAGTCATTTTGATTAATCTAATACGACAATAATGGTTTCGATCGAGAAAATGCAGGCAGGAGCAAGTGAACATGCGAAAGCGCTCGGTCCGAAAGGGTCGGAGGCTCACAAGGCAGCTGTGATTGGTGACACAGTTGGGGACCCTCTCAAAGACACTTCTGGTCCTTCACTCAATATCCTCATCAAGCTCATGGCTGTTGAGTCTTTGGTTTTTGCCCCATTCTTTGCTGCTCATGGTGGCCTCATCTTCAAGCATATATGACAACAATAACCAAACTTGTTCCTTTGGTAGCTAAACTAAATAATGTAGATTACACATCATTTGCCCATTTTGATGGTTTTGTTTGGGTGGATGCTCAAAAATCTAAGTGTCTTATAATGTTAACCGTTTTTCTCCTTTGAGCTGGTGGGAGAGAGAACAAGAGTGTCAGTAATTCAACAACATATGTCAACTAAAACATAGCCC

mRNA sequence

CTTCAGACTGCTGCACATCAAAACCCGAATTTGCTGTTGTTATATATGCCAGGAAACCTCAGGGAACATTGTGATCTTTGAAAATGGGTGGTTTGAGTGAGGGTCTGACACAGTTGCTGATACCTGCAGCTGCCTTGCTTGGTATTGGGTTTGCCCTTCTTCAATGGCTTTTGGTCTCAAGAGTTAAGATTTCTGACTACTCTGATATGGATGAAAGCCAAGAAAATAACTTGATTGAAGGAGGAGGAGAGGAAGGTATTGACGATTTTGAGGTAGTCCTAAAGTGTGCTGAAATTCAGAAGGCCATTTCTGTTGGGGCAACATCATTTTTATTCACCCAGTATAAGTACCTAGTTGTCTTCATGGTAGCATTTGGAGCCATCATTTTCCTGTTCCTTGGCTCAGTTAAGAGCTTCAGCACCAAAAGTGAACCTTGCACATACAAGAAGGGAAATATGTGTAAACCAGCTCTAGCCAATGCATTCTTTAGCACTATTGCCTTTTTGCTAGGTGGTCTTACCTCGGTTCTTTCTGGTTTCCTTGGAATGAAGATTGCAACTTATGCAAATGCCCGAACGAGTCTAGAAGCACGAAAAGGCATTGGAAAAGCATTTGTTATCGCTTTTCGCTCGGGTGCTGTGATGGGATTCCTTCTAGCTGCCAATGGCCTCCTTGTATTATATGTCTCCATCAATTTGTTTAAGCTGTATTACGGTGACGACTGGGAAGGACTGTATGAATCTATCACGGGTTATGGTCTTGGAGGTTCTTCTATGGCTCTATTTGGAAGAGTGGGGGGAGGCATATACACAAAAGCAGCTGATGTTGGAGCTGATCTTGTAGGGAAAATTGAAAAGAATATCCCTGAAGACGATCCACGAAACCCTGCTGTTATAGCTGACAATGTGGGTGACAATGTAGGAGACATAGCAGGAATGGGCTCTGACTTATTTGGATCTTATGCTGAATCAACCTGTGCAGCACTTTTTGTAGCATCAATATCATCCTTCGGCTTCACACATAACTATGCAGCCATGTTGTACCCTCTACTTATAAGCTCAATGGGCATTGTGATTTGCTTAATAACTACACTTTTCGCAACCGATCTCATTGAGATTAAGAAAGTGAGTGGAGTTGAACCATCATTAAAGCGACAGCTTCTTTTCTCCACTGTCCTGATGACAGCAGGGGTAGCTTTGGTCAGCTTGGTTGCTTTACCTTCGAAATTCACTCTCTACGATTTCGGCAACGACAAGGTCGTAAAGAACTGGCACCTTTTCTTCTGTGTTGTGACAGGATTATGGGCAGGACTTGTGATTGGATATACAACTGAATACTACACTAGTAATGCTTATAGTCCGGTGCAGGATGTGGCTGATTCCTGTAGAACCGGTGCCGCAACAAATGTGATATTTGGATTAGCTCTTGGTTACAAATCCGTCATAATTCCCATATTTTCGATCGCCTTATCCATTTACGTGAGCTTCCGCTTGGCAGCCATGTATGGAATTGCGATGGCTGCTTTAGGAATGCTTAGCACCATTGCCACTGGTCTAGCCATTGATGCATATGGACCCATTAGTGACAATGCAGGTGGAATAGCCGAAATGGCTGGAATGAGCCACGAGGTACGCGAGAGAACCGACGCTTTAGACGCTGCAGGAAATACAACTGCTGCCATAGGCAAGGGCTTTGCCATTGGATCTGCTGCTCTTGTTTCCCTCGCCTTGTTCGGTGCCTTTGTGAGCCGAGCAGGCATCGAAACAGTCGACGTCATGGACCCGAAAGTTTTCATTGGACTGATAGTAGGTGCCATGCTTCCATATTGGTTCTCAGCAATGACAATGAAGAGTGTTGGCAGTGCAGCGCTGAAGATGGTCGAGGAAGTTCGACGACAATTCAACTCGATCCCGGGGCTGATGGAAGGCAGGACAAAGCCGGACTACGCGAATTGTGTGAAGATATCGACGGATGCTTCACTTAGAGAGATGATCCCACCTGGAGCTCTGGTTTTGGTAACACCACTTATTGCTGGAACATTCTTTGGAGTTGAAACTCTTGCTGGGCTGCTGGCTGGTTCCCTTGTTTCTGGTGTGCAGGTTGCTATCTCTGCTTCAAACACAGGTGGGGCTTGGGATAATGCCAAAAAGTACATAGAGGCAGGAGCAAGTGAACATGCGAAAGCGCTCGGTCCGAAAGGGTCGGAGGCTCACAAGGCAGCTGTGATTGGTGACACAGTTGGGGACCCTCTCAAAGACACTTCTGGTCCTTCACTCAATATCCTCATCAAGCTCATGGCTGTTGAGTCTTTGGTTTTTGCCCCATTCTTTGCTGCTCATGGTGGCCTCATCTTCAAGCATATATGACAACAATAACCAAACTTGTTCCTTTGGTAGCTAAACTAAATAATGTAGATTACACATCATTTGCCCATTTTGATGGTTTTGTTTGGGTGGATGCTCAAAAATCTAAGTGTCTTATAATGTTAACCGTTTTTCTCCTTTGAGCTGGTGGGAGAGAGAACAAGAGTGTCAGTAATTCAACAACATATGTCAACTAAAACATAGCCC

Coding sequence (CDS)

ATGGGTGGTTTGAGTGAGGGTCTGACACAGTTGCTGATACCTGCAGCTGCCTTGCTTGGTATTGGGTTTGCCCTTCTTCAATGGCTTTTGGTCTCAAGAGTTAAGATTTCTGACTACTCTGATATGGATGAAAGCCAAGAAAATAACTTGATTGAAGGAGGAGGAGAGGAAGGTATTGACGATTTTGAGGTAGTCCTAAAGTGTGCTGAAATTCAGAAGGCCATTTCTGTTGGGGCAACATCATTTTTATTCACCCAGTATAAGTACCTAGTTGTCTTCATGGTAGCATTTGGAGCCATCATTTTCCTGTTCCTTGGCTCAGTTAAGAGCTTCAGCACCAAAAGTGAACCTTGCACATACAAGAAGGGAAATATGTGTAAACCAGCTCTAGCCAATGCATTCTTTAGCACTATTGCCTTTTTGCTAGGTGGTCTTACCTCGGTTCTTTCTGGTTTCCTTGGAATGAAGATTGCAACTTATGCAAATGCCCGAACGAGTCTAGAAGCACGAAAAGGCATTGGAAAAGCATTTGTTATCGCTTTTCGCTCGGGTGCTGTGATGGGATTCCTTCTAGCTGCCAATGGCCTCCTTGTATTATATGTCTCCATCAATTTGTTTAAGCTGTATTACGGTGACGACTGGGAAGGACTGTATGAATCTATCACGGGTTATGGTCTTGGAGGTTCTTCTATGGCTCTATTTGGAAGAGTGGGGGGAGGCATATACACAAAAGCAGCTGATGTTGGAGCTGATCTTGTAGGGAAAATTGAAAAGAATATCCCTGAAGACGATCCACGAAACCCTGCTGTTATAGCTGACAATGTGGGTGACAATGTAGGAGACATAGCAGGAATGGGCTCTGACTTATTTGGATCTTATGCTGAATCAACCTGTGCAGCACTTTTTGTAGCATCAATATCATCCTTCGGCTTCACACATAACTATGCAGCCATGTTGTACCCTCTACTTATAAGCTCAATGGGCATTGTGATTTGCTTAATAACTACACTTTTCGCAACCGATCTCATTGAGATTAAGAAAGTGAGTGGAGTTGAACCATCATTAAAGCGACAGCTTCTTTTCTCCACTGTCCTGATGACAGCAGGGGTAGCTTTGGTCAGCTTGGTTGCTTTACCTTCGAAATTCACTCTCTACGATTTCGGCAACGACAAGGTCGTAAAGAACTGGCACCTTTTCTTCTGTGTTGTGACAGGATTATGGGCAGGACTTGTGATTGGATATACAACTGAATACTACACTAGTAATGCTTATAGTCCGGTGCAGGATGTGGCTGATTCCTGTAGAACCGGTGCCGCAACAAATGTGATATTTGGATTAGCTCTTGGTTACAAATCCGTCATAATTCCCATATTTTCGATCGCCTTATCCATTTACGTGAGCTTCCGCTTGGCAGCCATGTATGGAATTGCGATGGCTGCTTTAGGAATGCTTAGCACCATTGCCACTGGTCTAGCCATTGATGCATATGGACCCATTAGTGACAATGCAGGTGGAATAGCCGAAATGGCTGGAATGAGCCACGAGGTACGCGAGAGAACCGACGCTTTAGACGCTGCAGGAAATACAACTGCTGCCATAGGCAAGGGCTTTGCCATTGGATCTGCTGCTCTTGTTTCCCTCGCCTTGTTCGGTGCCTTTGTGAGCCGAGCAGGCATCGAAACAGTCGACGTCATGGACCCGAAAGTTTTCATTGGACTGATAGTAGGTGCCATGCTTCCATATTGGTTCTCAGCAATGACAATGAAGAGTGTTGGCAGTGCAGCGCTGAAGATGGTCGAGGAAGTTCGACGACAATTCAACTCGATCCCGGGGCTGATGGAAGGCAGGACAAAGCCGGACTACGCGAATTGTGTGAAGATATCGACGGATGCTTCACTTAGAGAGATGATCCCACCTGGAGCTCTGGTTTTGGTAACACCACTTATTGCTGGAACATTCTTTGGAGTTGAAACTCTTGCTGGGCTGCTGGCTGGTTCCCTTGTTTCTGGTGTGCAGGTTGCTATCTCTGCTTCAAACACAGGTGGGGCTTGGGATAATGCCAAAAAGTACATAGAGGCAGGAGCAAGTGAACATGCGAAAGCGCTCGGTCCGAAAGGGTCGGAGGCTCACAAGGCAGCTGTGATTGGTGACACAGTTGGGGACCCTCTCAAAGACACTTCTGGTCCTTCACTCAATATCCTCATCAAGCTCATGGCTGTTGAGTCTTTGGTTTTTGCCCCATTCTTTGCTGCTCATGGTGGCCTCATCTTCAAGCATATATGA

Protein sequence

MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGIDDFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTYKKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAALFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLLFSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLAGLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI
Homology
BLAST of MC08g2204 vs. ExPASy Swiss-Prot
Match: P31414 (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 608/763 (79.69%), Postives = 679/763 (88.99%), Query Frame = 0

Query: 4   LSEGLTQLLIPAAALLGIGFALLQWLLVSRVKI-SDYSDMDESQENNLIEGGG------E 63
           L E  T++L+P  A++GI F+L QW +VSRVK+ SD         NN   G G      E
Sbjct: 7   LPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEEE 66

Query: 64  EGIDDFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSE 123
           EG++D  VV KCAEIQ AIS GATSFLFT+YKY+ VFM+ F A+IF+FLGSV+ FST ++
Sbjct: 67  EGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNK 126

Query: 124 PCTYKKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKA 183
           PCTY     CKPALA A FSTIAF+LG +TSVLSGFLGMKIATYANART+LEARKG+GKA
Sbjct: 127 PCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKA 186

Query: 184 FVIAFRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGR 243
           F++AFRSGAVMGFLLAA+GLLVLY++IN+FK+YYGDDWEGL+E+ITGYGLGGSSMALFGR
Sbjct: 187 FIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFGR 246

Query: 244 VGGGIYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 303
           VGGGIYTKAADVGADLVGKIE+NIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE+
Sbjct: 247 VGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAEA 306

Query: 304 TCAALFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLK 363
           +CAAL VASISSFG  H++ AM YPLLISSMGI++CLITTLFATD  EIK V  +EP+LK
Sbjct: 307 SCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPALK 366

Query: 364 RQLLFSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTT 423
            QL+ STV+MT G+A+VS V LP+ FT+++FG  KVVKNW LF CV  GLWAGL+IG+ T
Sbjct: 367 NQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFVT 426

Query: 424 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGI 483
           EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIF+IA+SI+VSF  AAMYG+
Sbjct: 427 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYGV 486

Query: 484 AMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKG 543
           A+AALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSH +RERTDALDAAGNTTAAIGKG
Sbjct: 487 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 546

Query: 544 FAIGSAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 603
           FAIGSAALVSLALFGAFVSRAGI TVDV+ PKV IGL+VGAMLPYWFSAMTMKSVGSAAL
Sbjct: 547 FAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAAL 606

Query: 604 KMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGV 663
           KMVEEVRRQFN+IPGLMEG  KPDYA CVKISTDAS++EMIPPG LV++TPLI G FFGV
Sbjct: 607 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFGV 666

Query: 664 ETLAGLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIG 723
           ETL+G+LAGSLVSGVQ+AISASNTGGAWDNAKKYIEAG SEHAK+LGPKGSE HKAAVIG
Sbjct: 667 ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVIG 726

Query: 724 DTVGDPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKH 760
           DT+GDPLKDTSGPSLNILIKLMAVESLVFAPFFA HGG++FK+
Sbjct: 727 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKY 769

BLAST of MC08g2204 vs. ExPASy Swiss-Prot
Match: P21616 (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 607/756 (80.29%), Postives = 677/756 (89.55%), Query Frame = 0

Query: 9   TQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMD------ESQENNLIEGGGEEGIDDF 68
           T++LIP  A++GI FAL QWLLVS+VK+S   D            + LIE   EEGI+D 
Sbjct: 11  TEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIE--EEEGINDH 70

Query: 69  EVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTYKK 128
            VV+KCAEIQ AIS GATSFLFT+YKY+ +FMVAF  +IFLFLGSV+ FST  + C+Y K
Sbjct: 71  NVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQACSYDK 130

Query: 129 GNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFR 188
              CKPALA A FST++FLLGG+TS++SGFLGMKIATYANART+LEARKG+GKAF+ AFR
Sbjct: 131 TKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKGVGKAFITAFR 190

Query: 189 SGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGGIY 248
           SGAVMGFLLAANGLLVLY++INLFK+YYGDDW GL+E+ITGYGLGGSSMALFGRVGGGIY
Sbjct: 191 SGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSSMALFGRVGGGIY 250

Query: 249 TKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAALF 308
           TKAADVGADLVGK+E+NIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES+CAAL 
Sbjct: 251 TKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALV 310

Query: 309 VASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLLFS 368
           VASISSFG  H   AMLYPL++SS+GI++CL+TTLFATD  EIK V  +EP+LK+QL+ S
Sbjct: 311 VASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPALKKQLVIS 370

Query: 369 TVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYTSN 428
           TVLMT GVA+VS VALP+ FT+++FG  K VK+W LF CV  GLWAGL+IG+ TEYYTSN
Sbjct: 371 TVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGLIIGFVTEYYTSN 430

Query: 429 AYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAALG 488
           AYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIF+IA+SI+VSF  AAMYGIA+AALG
Sbjct: 431 AYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTFAAMYGIAVAALG 490

Query: 489 MLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIGSA 548
           MLSTIATGLAIDAYGPISDNAGGIAEMAGMSH +RERTDALDAAGNTTAAIGKGFAIGSA
Sbjct: 491 MLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSA 550

Query: 549 ALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEV 608
           ALVSLALFGAFVSRA I TVDV+ PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEV
Sbjct: 551 ALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEV 610

Query: 609 RRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLAGL 668
           RRQFN+IPGLMEG  KPDYA CVKISTDAS++EMIPPGALV++TPL+ G  FGVETL+G+
Sbjct: 611 RRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVVGILFGVETLSGV 670

Query: 669 LAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDP 728
           LAGSLVSGVQ+AISASNTGGAWDNAKKYIEAGASEHA++LGPKGS+ HKAAVIGDT+GDP
Sbjct: 671 LAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCHKAAVIGDTIGDP 730

Query: 729 LKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFK 759
           LKDTSGPSLNILIKLMAVESLVFAPFFA HGGL+FK
Sbjct: 731 LKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFK 764

BLAST of MC08g2204 vs. ExPASy Swiss-Prot
Match: Q06572 (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 600/765 (78.43%), Postives = 684/765 (89.41%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENN-----LIEGGG 60
           M  L E  T++LIP   ++GI FA+ QW +VS+VK++  +    +   N     LIE   
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIE--E 60

Query: 61  EEGIDDFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKS 120
           EEG++D  VV+KCAEIQ AIS GATSFLFT Y+Y+ +FMV F AIIFLFLGS++ FSTK 
Sbjct: 61  EEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKG 120

Query: 121 EPCTYKKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGK 180
           +PCTY KG  CKPAL  A FST +FLLG +TS++SGFLGMKIATYANART+LEARKG+GK
Sbjct: 121 QPCTYSKGT-CKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVGK 180

Query: 181 AFVIAFRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFG 240
           AF+ AFRSGAVMGFLL+++GL+VLY++IN+FK+YYGDDWEGL+ESITGYGLGGSSMALFG
Sbjct: 181 AFITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMALFG 240

Query: 241 RVGGGIYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 300
           RVGGGIYTKAADVGADLVGK+E+NIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 241 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 300

Query: 301 STCAALFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSL 360
           S+CAAL VASISSFG  H++ AM YPLL+SS+GI++CL+TTLFATD  EIK  + +EP+L
Sbjct: 301 SSCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPAL 360

Query: 361 KRQLLFSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYT 420
           K+QL+ ST LMT GVA++S +ALP+KFT+++FG  K V NW LFFCV  GLWAGL+IG+ 
Sbjct: 361 KKQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLIIGFV 420

Query: 421 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYG 480
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIF+IA+SIYVSF +AAMYG
Sbjct: 421 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAAMYG 480

Query: 481 IAMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGK 540
           IAMAALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSH +RERTDALDAAGNTTAAIGK
Sbjct: 481 IAMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 540

Query: 541 GFAIGSAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 600
           GFAIGSAALVSLALFGAFVSRAG++ VDV+ PKVFIGLIVGAMLPYWFSAMTMKSVGSAA
Sbjct: 541 GFAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 600

Query: 601 LKMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFG 660
           LKMVEEVRRQFN+IPGLMEG  KPDYA CVKISTDAS++EMIPPGALV++TPLI GT FG
Sbjct: 601 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGTLFG 660

Query: 661 VETLAGLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVI 720
           VETL+G+LAG+LVSGVQ+AISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+ HKAAVI
Sbjct: 661 VETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKAAVI 720

Query: 721 GDTVGDPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 761
           GDT+GDPLKDTSGPSLNILIKLMAVESLVFAPFFA +GGL+FK+I
Sbjct: 721 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYGGLLFKYI 762

BLAST of MC08g2204 vs. ExPASy Swiss-Prot
Match: Q72Q29 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 2.5e-197
Identity = 390/693 (56.28%), Postives = 495/693 (71.43%), Query Frame = 0

Query: 67  KCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTYKKGNMC 126
           K  EI  AIS GA +FL  +YK + +F+     +I L L                  N  
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLL-----------------DNPG 101

Query: 127 KPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFRSGAV 186
                +  ++ IAF+ G L S +SGF+GMKIAT  N RT+  A+  + KAF +AF SGAV
Sbjct: 102 SEGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 187 MGFLLAANGLLVLYVSINLFK-LYYGDDWEGLYESITGYGLGGSSMALFGRVGGGIYTKA 246
           MGF L    +L + V   +F  +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYTKA
Sbjct: 162 MGFGLVGLAILGMIVLFLVFTGMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYTKA 221

Query: 247 ADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAALFVAS 306
           ADVGADLVGK+EK IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE+TCAAL + +
Sbjct: 222 ADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVIGA 281

Query: 307 ISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLLFSTVL 366
            +S   + +  A+LYPLLIS+ GI   ++T+  A     +K+   VE +LK QL  ST+L
Sbjct: 282 TAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVSTLL 341

Query: 367 MTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYTSNAYS 426
           +   +  V+   +   F +      K +  W ++  +V GL++G+ IG  TEYYTS++Y 
Sbjct: 342 VAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHSYK 401

Query: 427 PVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAALGMLS 486
           PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +  LA MYGIA+AALGM+S
Sbjct: 402 PVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGMIS 461

Query: 487 TIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIGSAALV 546
           TIA GL IDAYGP+SDNAGGIAEMA +  EVR+RTD LDAAGNTTAAIGKGFAIGSAAL 
Sbjct: 462 TIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAALT 521

Query: 547 SLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQ 606
           SLALF AF++R    +++V++ +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR+Q
Sbjct: 522 SLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVRKQ 581

Query: 607 FNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLAGLLAG 666
           F  IPG+MEG+ KPDY  CV IST A+LREMI PG LVL+TP++ G  FGV+TLAG+LAG
Sbjct: 582 FKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVLAG 641

Query: 667 SLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDPLKD 726
           +LV+GV +AISA+N+GG WDNAKKYIE       K  G KGS+ HKAAV+GDTVGDP KD
Sbjct: 642 ALVAGVVLAISAANSGGGWDNAKKYIE-------KKAGGKGSDQHKAAVVGDTVGDPFKD 701

Query: 727 TSGPSLNILIKLMAVESLVFAPFFAAHGGLIFK 759
           TSGPS+NILIKLMA+ SLVFA FF   GGLIFK
Sbjct: 702 TSGPSINILIKLMAITSLVFAEFFVQQGGLIFK 701

BLAST of MC08g2204 vs. ExPASy Swiss-Prot
Match: Q8F641 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 2.5e-197
Identity = 390/693 (56.28%), Postives = 495/693 (71.43%), Query Frame = 0

Query: 67  KCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTYKKGNMC 126
           K  EI  AIS GA +FL  +YK + +F+     +I L L                  N  
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLL-----------------DNPG 101

Query: 127 KPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFRSGAV 186
                +  ++ IAF+ G L S +SGF+GMKIAT  N RT+  A+  + KAF +AF SGAV
Sbjct: 102 SEGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 187 MGFLLAANGLLVLYVSINLFK-LYYGDDWEGLYESITGYGLGGSSMALFGRVGGGIYTKA 246
           MGF L    +L + V   +F  +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYTKA
Sbjct: 162 MGFGLVGLAILGMIVLFLVFTGMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYTKA 221

Query: 247 ADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAALFVAS 306
           ADVGADLVGK+EK IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE+TCAAL + +
Sbjct: 222 ADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVIGA 281

Query: 307 ISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLLFSTVL 366
            +S   + +  A+LYPLLIS+ GI   ++T+  A     +K+   VE +LK QL  ST+L
Sbjct: 282 TAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVSTLL 341

Query: 367 MTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYTSNAYS 426
           +   +  V+   +   F +      K +  W ++  +V GL++G+ IG  TEYYTS++Y 
Sbjct: 342 VAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHSYK 401

Query: 427 PVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAALGMLS 486
           PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +  LA MYGIA+AALGM+S
Sbjct: 402 PVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGMIS 461

Query: 487 TIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIGSAALV 546
           TIA GL IDAYGP+SDNAGGIAEMA +  EVR+RTD LDAAGNTTAAIGKGFAIGSAAL 
Sbjct: 462 TIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAALT 521

Query: 547 SLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQ 606
           SLALF AF++R    +++V++ +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR+Q
Sbjct: 522 SLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVRKQ 581

Query: 607 FNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLAGLLAG 666
           F  IPG+MEG+ KPDY  CV IST A+LREMI PG LVL+TP++ G  FGV+TLAG+LAG
Sbjct: 582 FKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVLAG 641

Query: 667 SLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDPLKD 726
           +LV+GV +AISA+N+GG WDNAKKYIE       K  G KGS+ HKAAV+GDTVGDP KD
Sbjct: 642 ALVAGVVLAISAANSGGGWDNAKKYIE-------KKAGGKGSDQHKAAVVGDTVGDPFKD 701

Query: 727 TSGPSLNILIKLMAVESLVFAPFFAAHGGLIFK 759
           TSGPS+NILIKLMA+ SLVFA FF   GGLIFK
Sbjct: 702 TSGPSINILIKLMAITSLVFAEFFVQQGGLIFK 701

BLAST of MC08g2204 vs. NCBI nr
Match: XP_022156348.1 (pyrophosphate-energized vacuolar membrane proton pump 1-like [Momordica charantia])

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 760/760 (100.00%), Postives = 760/760 (100.00%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID
Sbjct: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
           KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA
Sbjct: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
           FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760

BLAST of MC08g2204 vs. NCBI nr
Match: KAG6586225.1 (Pyrophosphate-energized vacuolar membrane proton pump 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1362 bits (3526), Expect = 0.0
Identity = 726/760 (95.53%), Postives = 739/760 (97.24%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGL Q+LIPAAALLGIGFA LQW+LVSRVK+S Y+D DESQENNLIE G EEG +
Sbjct: 1   MGGLSEGLIQVLIPAAALLGIGFAFLQWVLVSRVKVSGYAD-DESQENNLIESGHEEGFE 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGA+IFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAVIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAF LG LTSVLSGFLGMKIATYANARTSLEARKGIGKAF IA
Sbjct: 121 NKGQMCKPALANAIFSTIAFFLGALTSVLSGFLGMKIATYANARTSLEARKGIGKAFTIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAM YPLLISSMGIVICLITTLFATDL+E+KKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMSYPLLISSMGIVICLITTLFATDLVEVKKVSEIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMTAG ALVS VALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 ISTVLMTAGAALVSFVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVI+PIFSIALSIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIVPIFSIALSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETV+VM+PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVNVMNPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVE+LVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVEALVFAPFFAAHGGLIFKHI 759

BLAST of MC08g2204 vs. NCBI nr
Match: XP_022938207.1 (pyrophosphate-energized vacuolar membrane proton pump 1-like [Cucurbita moschata])

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 724/760 (95.26%), Postives = 737/760 (96.97%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MG LSEGL Q+LIPAAALLGIGFA LQW+LVS VK+S Y+D DESQENNLIE G EEG +
Sbjct: 1   MGALSEGLIQVLIPAAALLGIGFAFLQWVLVSGVKVSGYAD-DESQENNLIESGHEEGFE 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGA+IFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAVIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAF LG LTSVLSGFLGMKIATYANARTSLEARKGIGKAF IA
Sbjct: 121 NKGQMCKPALANAIFSTIAFFLGALTSVLSGFLGMKIATYANARTSLEARKGIGKAFTIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAM YPLLISSMGIVICLITTLFATDL+E+KKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMSYPLLISSMGIVICLITTLFATDLVEVKKVSEIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMTAG ALVS VALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 ISTVLMTAGAALVSFVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVI+PIFSIALSIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIVPIFSIALSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETV+VM+PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVNVMNPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVE+LVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVEALVFAPFFAAHGGLIFKHI 759

BLAST of MC08g2204 vs. NCBI nr
Match: XP_008453661.1 (PREDICTED: pyrophosphate-energized vacuolar membrane proton pump 1-like [Cucumis melo])

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 726/760 (95.53%), Postives = 737/760 (96.97%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVK+  Y D DESQENNLIEGG EEGID
Sbjct: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKVYSYPD-DESQENNLIEGGQEEGID 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           D EVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DVEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAFLLG LTSVLSGFLGMKIATYANARTSLEAR+GIG+AFVIA
Sbjct: 121 NKGQMCKPALANAIFSTIAFLLGALTSVLSGFLGMKIATYANARTSLEARRGIGRAFVIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFG THNYAAM YPLLISSMGIVICLITTLFATDLIEIKKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGSTHNYAAMSYPLLISSMGIVICLITTLFATDLIEIKKVSDIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMT GVALVS VALPSKFTLYDFGNDKVVKNWH+FFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 ISTVLMTVGVALVSFVALPSKFTLYDFGNDKVVKNWHIFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRL AMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLGAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHE+RERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEIRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRA IETV+VM+PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRADIETVNVMNPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASL+EMIPPG LVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLKEMIPPGGLVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAG SEHAKALGPKGS+AHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGRSEHAKALGPKGSDAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGG+IFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGIIFKHI 759

BLAST of MC08g2204 vs. NCBI nr
Match: XP_022965594.1 (pyrophosphate-energized vacuolar membrane proton pump 1-like [Cucurbita maxima])

HSP 1 Score: 1357 bits (3512), Expect = 0.0
Identity = 723/760 (95.13%), Postives = 738/760 (97.11%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGL QLLIPAAALLGIGFA LQW+LVSRVK+S Y+D DESQENNLIE G EEG +
Sbjct: 1   MGGLSEGLIQLLIPAAALLGIGFAFLQWVLVSRVKVSGYAD-DESQENNLIETGHEEGFE 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAF LG LTSVLSGFLGMKIATYANARTSLEARKGIGKAF IA
Sbjct: 121 NKGQMCKPALANAIFSTIAFFLGALTSVLSGFLGMKIATYANARTSLEARKGIGKAFTIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAM YPLLISSMGIVICLITTLFATDL+E+KKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMSYPLLISSMGIVICLITTLFATDLVEVKKVSEIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMTAG ALVS VALPS+F LYDFGNDKVVKNWHLFFCVVTGLWAGL+IGYTTEYYT
Sbjct: 361 ISTVLMTAGAALVSFVALPSRFKLYDFGNDKVVKNWHLFFCVVTGLWAGLIIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIA+SIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIAVSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETV+VM+P+VFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVNVMNPRVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVL+TPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLITPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVE+LVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVEALVFAPFFAAHGGLIFKHI 759

BLAST of MC08g2204 vs. ExPASy TrEMBL
Match: A0A6J1DQD8 (H(+)-exporting diphosphatase OS=Momordica charantia OX=3673 GN=LOC111023264 PE=3 SV=1)

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 760/760 (100.00%), Postives = 760/760 (100.00%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID
Sbjct: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
           KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA
Sbjct: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
           FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760

BLAST of MC08g2204 vs. ExPASy TrEMBL
Match: A0A6J1FDE8 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111444356 PE=3 SV=1)

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 724/760 (95.26%), Postives = 737/760 (96.97%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MG LSEGL Q+LIPAAALLGIGFA LQW+LVS VK+S Y+D DESQENNLIE G EEG +
Sbjct: 1   MGALSEGLIQVLIPAAALLGIGFAFLQWVLVSGVKVSGYAD-DESQENNLIESGHEEGFE 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGA+IFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAVIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAF LG LTSVLSGFLGMKIATYANARTSLEARKGIGKAF IA
Sbjct: 121 NKGQMCKPALANAIFSTIAFFLGALTSVLSGFLGMKIATYANARTSLEARKGIGKAFTIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAM YPLLISSMGIVICLITTLFATDL+E+KKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMSYPLLISSMGIVICLITTLFATDLVEVKKVSEIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMTAG ALVS VALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 ISTVLMTAGAALVSFVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVI+PIFSIALSIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIVPIFSIALSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETV+VM+PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVNVMNPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVE+LVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVEALVFAPFFAAHGGLIFKHI 759

BLAST of MC08g2204 vs. ExPASy TrEMBL
Match: A0A1S3BXZ5 (H(+)-exporting diphosphatase OS=Cucumis melo OX=3656 GN=LOC103494311 PE=3 SV=1)

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 726/760 (95.53%), Postives = 737/760 (96.97%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVK+  Y D DESQENNLIEGG EEGID
Sbjct: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKVYSYPD-DESQENNLIEGGQEEGID 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           D EVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DVEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAFLLG LTSVLSGFLGMKIATYANARTSLEAR+GIG+AFVIA
Sbjct: 121 NKGQMCKPALANAIFSTIAFLLGALTSVLSGFLGMKIATYANARTSLEARRGIGRAFVIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFG THNYAAM YPLLISSMGIVICLITTLFATDLIEIKKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGSTHNYAAMSYPLLISSMGIVICLITTLFATDLIEIKKVSDIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMT GVALVS VALPSKFTLYDFGNDKVVKNWH+FFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 ISTVLMTVGVALVSFVALPSKFTLYDFGNDKVVKNWHIFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRL AMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLGAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHE+RERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEIRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRA IETV+VM+PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRADIETVNVMNPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASL+EMIPPG LVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLKEMIPPGGLVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAG SEHAKALGPKGS+AHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGRSEHAKALGPKGSDAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGG+IFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGIIFKHI 759

BLAST of MC08g2204 vs. ExPASy TrEMBL
Match: A0A6J1HRF4 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111465445 PE=3 SV=1)

HSP 1 Score: 1357 bits (3512), Expect = 0.0
Identity = 723/760 (95.13%), Postives = 738/760 (97.11%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGL QLLIPAAALLGIGFA LQW+LVSRVK+S Y+D DESQENNLIE G EEG +
Sbjct: 1   MGGLSEGLIQLLIPAAALLGIGFAFLQWVLVSRVKVSGYAD-DESQENNLIETGHEEGFE 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           DFEVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DFEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAF LG LTSVLSGFLGMKIATYANARTSLEARKGIGKAF IA
Sbjct: 121 NKGQMCKPALANAIFSTIAFFLGALTSVLSGFLGMKIATYANARTSLEARKGIGKAFTIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFGFTHNYAAM YPLLISSMGIVICLITTLFATDL+E+KKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGFTHNYAAMSYPLLISSMGIVICLITTLFATDLVEVKKVSEIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMTAG ALVS VALPS+F LYDFGNDKVVKNWHLFFCVVTGLWAGL+IGYTTEYYT
Sbjct: 361 ISTVLMTAGAALVSFVALPSRFKLYDFGNDKVVKNWHLFFCVVTGLWAGLIIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIA+SIYVSFRLAAMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIAVSIYVSFRLAAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRAGIETV+VM+P+VFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRAGIETVNVMNPRVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVL+TPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLITPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVE+LVFAPFFAAHGGLIFKHI
Sbjct: 721 DPLKDTSGPSLNILIKLMAVEALVFAPFFAAHGGLIFKHI 759

BLAST of MC08g2204 vs. ExPASy TrEMBL
Match: A0A0A0LM24 (H(+)-exporting diphosphatase OS=Cucumis sativus OX=3659 GN=Csa_2G033950 PE=3 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 721/760 (94.87%), Postives = 735/760 (96.71%), Query Frame = 0

Query: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKISDYSDMDESQENNLIEGGGEEGID 60
           MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVK+  Y D DESQENNLIEGG EEGID
Sbjct: 1   MGGLSEGLTQLLIPAAALLGIGFALLQWLLVSRVKVYSYPD-DESQENNLIEGGQEEGID 60

Query: 61  DFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTY 120
           D EVV KCAEIQKAISVGATSFLFTQYKYLVVFM AFGAIIFLFLGSVKSFSTKSEPCTY
Sbjct: 61  DVEVVAKCAEIQKAISVGATSFLFTQYKYLVVFMGAFGAIIFLFLGSVKSFSTKSEPCTY 120

Query: 121 KKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIA 180
            KG MCKPALANA FSTIAFLLG LTSVLSGFLGMKIATYANARTSLEAR+GIG+AFVIA
Sbjct: 121 NKGQMCKPALANAIFSTIAFLLGALTSVLSGFLGMKIATYANARTSLEARRGIGRAFVIA 180

Query: 181 FRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240
           FRSGAVMGFLLAANGLLVLY SINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG
Sbjct: 181 FRSGAVMGFLLAANGLLVLYASINLFKLYYGDDWEGLYESITGYGLGGSSMALFGRVGGG 240

Query: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300
           IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA
Sbjct: 241 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 300

Query: 301 LFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQLL 360
           LFVASISSFG THNY AM YPLLISSMGIVICLITTLFATD IEIKKVS +EPSLKRQLL
Sbjct: 301 LFVASISSFGSTHNYVAMSYPLLISSMGIVICLITTLFATDFIEIKKVSQIEPSLKRQLL 360

Query: 361 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTTEYYT 420
            STVLMT GVALVS VALPSKFTLYDFG+DKVVKNWH+FFCVVTGLWAGLVIGYTTEYYT
Sbjct: 361 ISTVLMTVGVALVSFVALPSKFTLYDFGSDKVVKNWHIFFCVVTGLWAGLVIGYTTEYYT 420

Query: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGIAMAA 480
           SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRL AMYGIAMAA
Sbjct: 421 SNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLGAMYGIAMAA 480

Query: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKGFAIG 540
           LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHE+RERTDALDAAGNTTAAIGKGFAIG
Sbjct: 481 LGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEIRERTDALDAAGNTTAAIGKGFAIG 540

Query: 541 SAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600
           SAALVSLALFGAFVSRA IETV+VM+PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE
Sbjct: 541 SAALVSLALFGAFVSRADIETVNVMNPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVE 600

Query: 601 EVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGVETLA 660
           EVRRQFN+IPGLMEGRTKPDYANCVKISTDASL+EMIPPG LVLVTPLIAGTFFGVETLA
Sbjct: 601 EVRRQFNTIPGLMEGRTKPDYANCVKISTDASLKEMIPPGGLVLVTPLIAGTFFGVETLA 660

Query: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVG 720
           GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAG SEHAKALGPKGS+AHKAAVIGDTVG
Sbjct: 661 GLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGRSEHAKALGPKGSDAHKAAVIGDTVG 720

Query: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKHI 760
           DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGG+IFK+I
Sbjct: 721 DPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGIIFKYI 759

BLAST of MC08g2204 vs. TAIR 10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 608/763 (79.69%), Postives = 679/763 (88.99%), Query Frame = 0

Query: 4   LSEGLTQLLIPAAALLGIGFALLQWLLVSRVKI-SDYSDMDESQENNLIEGGG------E 63
           L E  T++L+P  A++GI F+L QW +VSRVK+ SD         NN   G G      E
Sbjct: 7   LPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEEE 66

Query: 64  EGIDDFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSE 123
           EG++D  VV KCAEIQ AIS GATSFLFT+YKY+ VFM+ F A+IF+FLGSV+ FST ++
Sbjct: 67  EGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNK 126

Query: 124 PCTYKKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKA 183
           PCTY     CKPALA A FSTIAF+LG +TSVLSGFLGMKIATYANART+LEARKG+GKA
Sbjct: 127 PCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKA 186

Query: 184 FVIAFRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGR 243
           F++AFRSGAVMGFLLAA+GLLVLY++IN+FK+YYGDDWEGL+E+ITGYGLGGSSMALFGR
Sbjct: 187 FIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFGR 246

Query: 244 VGGGIYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 303
           VGGGIYTKAADVGADLVGKIE+NIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE+
Sbjct: 247 VGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAEA 306

Query: 304 TCAALFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLK 363
           +CAAL VASISSFG  H++ AM YPLLISSMGI++CLITTLFATD  EIK V  +EP+LK
Sbjct: 307 SCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPALK 366

Query: 364 RQLLFSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTT 423
            QL+ STV+MT G+A+VS V LP+ FT+++FG  KVVKNW LF CV  GLWAGL+IG+ T
Sbjct: 367 NQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFVT 426

Query: 424 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGI 483
           EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIF+IA+SI+VSF  AAMYG+
Sbjct: 427 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYGV 486

Query: 484 AMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKG 543
           A+AALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSH +RERTDALDAAGNTTAAIGKG
Sbjct: 487 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 546

Query: 544 FAIGSAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 603
           FAIGSAALVSLALFGAFVSRAGI TVDV+ PKV IGL+VGAMLPYWFSAMTMKSVGSAAL
Sbjct: 547 FAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAAL 606

Query: 604 KMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPPGALVLVTPLIAGTFFGV 663
           KMVEEVRRQFN+IPGLMEG  KPDYA CVKISTDAS++EMIPPG LV++TPLI G FFGV
Sbjct: 607 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFGV 666

Query: 664 ETLAGLLAGSLVSGVQVAISASNTGGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIG 723
           ETL+G+LAGSLVSGVQ+AISASNTGGAWDNAKKYIEAG SEHAK+LGPKGSE HKAAVIG
Sbjct: 667 ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVIG 726

Query: 724 DTVGDPLKDTSGPSLNILIKLMAVESLVFAPFFAAHGGLIFKH 760
           DT+GDPLKDTSGPSLNILIKLMAVESLVFAPFFA HGG++FK+
Sbjct: 727 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKY 769

BLAST of MC08g2204 vs. TAIR 10
Match: AT1G15690.2 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 937.6 bits (2422), Expect = 6.4e-273
Identity = 490/626 (78.27%), Postives = 549/626 (87.70%), Query Frame = 0

Query: 4   LSEGLTQLLIPAAALLGIGFALLQWLLVSRVKI-SDYSDMDESQENNLIEGGG------E 63
           L E  T++L+P  A++GI F+L QW +VSRVK+ SD         NN   G G      E
Sbjct: 7   LPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEEE 66

Query: 64  EGIDDFEVVLKCAEIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSE 123
           EG++D  VV KCAEIQ AIS GATSFLFT+YKY+ VFM+ F A+IF+FLGSV+ FST ++
Sbjct: 67  EGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDNK 126

Query: 124 PCTYKKGNMCKPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKA 183
           PCTY     CKPALA A FSTIAF+LG +TSVLSGFLGMKIATYANART+LEARKG+GKA
Sbjct: 127 PCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGKA 186

Query: 184 FVIAFRSGAVMGFLLAANGLLVLYVSINLFKLYYGDDWEGLYESITGYGLGGSSMALFGR 243
           F++AFRSGAVMGFLLAA+GLLVLY++IN+FK+YYGDDWEGL+E+ITGYGLGGSSMALFGR
Sbjct: 187 FIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFGR 246

Query: 244 VGGGIYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 303
           VGGGIYTKAADVGADLVGKIE+NIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE+
Sbjct: 247 VGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAEA 306

Query: 304 TCAALFVASISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLK 363
           +CAAL VASISSFG  H++ AM YPLLISSMGI++CLITTLFATD  EIK V  +EP+LK
Sbjct: 307 SCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPALK 366

Query: 364 RQLLFSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLFFCVVTGLWAGLVIGYTT 423
            QL+ STV+MT G+A+VS V LP+ FT+++FG  KVVKNW LF CV  GLWAGL+IG+ T
Sbjct: 367 NQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFVT 426

Query: 424 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRLAAMYGI 483
           EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIF+IA+SI+VSF  AAMYG+
Sbjct: 427 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYGV 486

Query: 484 AMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDAAGNTTAAIGKG 543
           A+AALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSH +RERTDALDAAGNTTAAIGKG
Sbjct: 487 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 546

Query: 544 FAIGSAALVSLALFGAFVSRAGIETVDVMDPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 603
           FAIGSAALVSLALFGAFVSRAGI TVDV+ PKV IGL+VGAMLPYWFSAMTMKSVGSAAL
Sbjct: 547 FAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAAL 606

Query: 604 KMVEEVRRQFNSIPGLMEGRTKPDYA 623
           KMVEEVRRQFN+IPGLMEG  KPDYA
Sbjct: 607 KMVEEVRRQFNTIPGLMEGTAKPDYA 632

BLAST of MC08g2204 vs. TAIR 10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 425.2 bits (1092), Expect = 1.1e-118
Identity = 286/722 (39.61%), Postives = 405/722 (56.09%), Query Frame = 0

Query: 70  EIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTYKKGNMCKPA 129
           EI  AI  GA  F  TQY   +  M    A + L +   +S + + E     +       
Sbjct: 101 EISDAIRDGAEGFFRTQYS-TISKMAILLAFVILCIYLFRSLTPQQEAAGLGRA------ 160

Query: 130 LANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFRSGAVMGF 189
             +A+ +  AFLLG L S ++G++GM ++  AN R S  AR+   +A  IA R+G     
Sbjct: 161 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 220

Query: 190 LLAANGLLVLYVSINLFKLYYGDDWEG------LYESITGYGLGGSSMALFGRVGGGIYT 249
           ++    ++ + +  + F ++ G    G      L   + GYG G S +ALF ++GGGIYT
Sbjct: 221 VVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIYT 280

Query: 250 KAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAALFV 309
           K ADVGADLVGK+E+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A+ +
Sbjct: 281 KGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 340

Query: 310 AS--ISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVE-PSLKRQLL 369
                           +L+PL++ S  ++I  I  L      +    S VE P    Q  
Sbjct: 341 GGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQKG 400

Query: 370 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLF-FCVVTGLWAGLVIGYTTEYY 429
           +S  ++ A    V      +++ LY    ++    W  F  C + G+    +  + ++YY
Sbjct: 401 YSLTIILA----VITFGASTRWLLY---TEQAPSAWFNFALCGLVGIITAYIFVWISKYY 460

Query: 430 TSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRL--------- 489
           T   + PV+ +A +  TG  TN+I G++LG +S  +P+ +I+++I  ++ L         
Sbjct: 461 TDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSGLVDE 520

Query: 490 -----AAMYGIAMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDA 549
                  ++G A+A +GMLST A  L +D +GPI+DNAGGI EM+     VRE TD LDA
Sbjct: 521 NGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITDLLDA 580

Query: 550 AGNTTAAIGKGFAIGSAALVSLALFGAFV------SRAGIETVDVMDPKVFIGLIVGAML 609
            GNTT A  KGFAIGSAAL S  LF A++      +    + VD+  P+VF+G ++GAML
Sbjct: 581 VGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLLGAML 640

Query: 610 PYWFSAMTMKSVGSAALKMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPP 669
            + FSA    +VG  A ++V EVRRQF   PG+ME + KPDY+ CV I   A+LREMI P
Sbjct: 641 IFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALREMIKP 700

Query: 670 GALVLVTPLIAGTFF------------GVETLAGLLAGSLVSGVQVAISASNTGGAWDNA 729
           GAL + +P++ G  F            G + +A +L  + V G+ +A+  +  GGAWDNA
Sbjct: 701 GALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGAWDNA 760

Query: 730 KKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAP 750
           KKYIE G      ALG KGSEAHKAAV GDTVGDP KDT+GPS+++LIK++A  +LV AP
Sbjct: 761 KKYIETG------ALGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITLVMAP 801

BLAST of MC08g2204 vs. TAIR 10
Match: AT1G78920.2 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 425.2 bits (1092), Expect = 1.1e-118
Identity = 286/722 (39.61%), Postives = 405/722 (56.09%), Query Frame = 0

Query: 70  EIQKAISVGATSFLFTQYKYLVVFMVAFGAIIFLFLGSVKSFSTKSEPCTYKKGNMCKPA 129
           EI  AI  GA  F  TQY   +  M    A + L +   +S + + E     +       
Sbjct: 101 EISDAIRDGAEGFFRTQYS-TISKMAILLAFVILCIYLFRSLTPQQEAAGLGRA------ 160

Query: 130 LANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFRSGAVMGF 189
             +A+ +  AFLLG L S ++G++GM ++  AN R S  AR+   +A  IA R+G     
Sbjct: 161 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 220

Query: 190 LLAANGLLVLYVSINLFKLYYGDDWEG------LYESITGYGLGGSSMALFGRVGGGIYT 249
           ++    ++ + +  + F ++ G    G      L   + GYG G S +ALF ++GGGIYT
Sbjct: 221 VVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIYT 280

Query: 250 KAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAALFV 309
           K ADVGADLVGK+E+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A+ +
Sbjct: 281 KGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 340

Query: 310 AS--ISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVE-PSLKRQLL 369
                           +L+PL++ S  ++I  I  L      +    S VE P    Q  
Sbjct: 341 GGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQKG 400

Query: 370 FSTVLMTAGVALVSLVALPSKFTLYDFGNDKVVKNWHLF-FCVVTGLWAGLVIGYTTEYY 429
           +S  ++ A    V      +++ LY    ++    W  F  C + G+    +  + ++YY
Sbjct: 401 YSLTIILA----VITFGASTRWLLY---TEQAPSAWFNFALCGLVGIITAYIFVWISKYY 460

Query: 430 TSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRL--------- 489
           T   + PV+ +A +  TG  TN+I G++LG +S  +P+ +I+++I  ++ L         
Sbjct: 461 TDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSGLVDE 520

Query: 490 -----AAMYGIAMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRERTDALDA 549
                  ++G A+A +GMLST A  L +D +GPI+DNAGGI EM+     VRE TD LDA
Sbjct: 521 NGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITDLLDA 580

Query: 550 AGNTTAAIGKGFAIGSAALVSLALFGAFV------SRAGIETVDVMDPKVFIGLIVGAML 609
            GNTT A  KGFAIGSAAL S  LF A++      +    + VD+  P+VF+G ++GAML
Sbjct: 581 VGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLLGAML 640

Query: 610 PYWFSAMTMKSVGSAALKMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDASLREMIPP 669
            + FSA    +VG  A ++V EVRRQF   PG+ME + KPDY+ CV I   A+LREMI P
Sbjct: 641 IFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALREMIKP 700

Query: 670 GALVLVTPLIAGTFF------------GVETLAGLLAGSLVSGVQVAISASNTGGAWDNA 729
           GAL + +P++ G  F            G + +A +L  + V G+ +A+  +  GGAWDNA
Sbjct: 701 GALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGAWDNA 760

Query: 730 KKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAVESLVFAP 750
           KKYIE G      ALG KGSEAHKAAV GDTVGDP KDT+GPS+++LIK++A  +LV AP
Sbjct: 761 KKYIETG------ALGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITLVMAP 801

BLAST of MC08g2204 vs. TAIR 10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 4.1e-118
Identity = 288/729 (39.51%), Postives = 414/729 (56.79%), Query Frame = 0

Query: 70  EIQKAISVGATSFLFTQYKYL--VVFMVAFGAI-IFLFLGSVKSFSTKSEPCTYKKGNMC 129
           +I  AI  GA  FL TQY  +  + F++AF  + I+LF    ++ + + E     +    
Sbjct: 150 QISDAIRDGAEGFLRTQYGTISKMAFLLAFVILCIYLF----RNLTPQQEASGLGR---- 209

Query: 130 KPALANAFFSTIAFLLGGLTSVLSGFLGMKIATYANARTSLEARKGIGKAFVIAFRSGAV 189
                +A+ +  AFLLG L S ++G++GM ++  AN R S  AR+   +A  IA R+G  
Sbjct: 210 ---TMSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 269

Query: 190 MGFLLAANGLLVLYVSINLFKLYYGDDWEG------LYESITGYGLGGSSMALFGRVGGG 249
              ++    ++ + +  + F ++   D  G      L   + GYG G S +ALF ++GGG
Sbjct: 270 SALVVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGG 329

Query: 250 IYTKAADVGADLVGKIEKNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESTCAA 309
           IYTK ADVGADLVGK+E  IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 330 IYTKGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 389

Query: 310 LFVAS--ISSFGFTHNYAAMLYPLLISSMGIVICLITTLFATDLIEIKKVSGVEPSLKRQ 369
           + +                +L+PL++ S  +VI  I       ++ IK       S+K  
Sbjct: 390 MILGGTMAQKCKIEDPSGFILFPLVVHSFDLVISSI------GILSIKGTRNA--SVKSP 449

Query: 370 LLFSTVLMTAGVALVSLVAL-----PSKFTLYDFGNDKVVKNW-HLFFCVVTGLWAGLVI 429
           +    V++  G +L  ++A+      +++ LY    ++    W + F C + G+    V 
Sbjct: 450 VEDPMVVLQKGYSLTIILAVLTFGASTRWLLY---TEQAPSAWLNFFMCGLVGIITAYVF 509

Query: 430 GYTTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFSIALSIYVSFRL-- 489
            + + YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+++I  +F L  
Sbjct: 510 VWISRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIISAFWLGN 569

Query: 490 ------------AAMYGIAMAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHEVRE 549
                         ++G A+A +GMLST A  L +D +GPI+DNAGGI EM+     VRE
Sbjct: 570 TSGLIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVRE 629

Query: 550 RTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFV------SRAGIETVDVMDPKVFIG 609
            TD LDA GNTT A  KGFAIGSAAL S  LF A++      +    + VD+  P+VFIG
Sbjct: 630 ITDVLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFIG 689

Query: 610 LIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNSIPGLMEGRTKPDYANCVKISTDAS 669
            ++GAML + FSA    +VG  A ++V EVRRQF   PG+M+ + KPDY  CV I   ++
Sbjct: 690 GLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVAIVASSA 749

Query: 670 LREMIPPGALVLVTPLIAGTFF------------GVETLAGLLAGSLVSGVQVAISASNT 729
           LREMI PGAL +++P+  G  F            G + +A +L  + V G+ +A+  +  
Sbjct: 750 LREMIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMALFLNTA 809

Query: 730 GGAWDNAKKYIEAGASEHAKALGPKGSEAHKAAVIGDTVGDPLKDTSGPSLNILIKLMAV 750
           GGAWDNAKKYIE G      ALG KGS++HKAAV GDTVGDP KDT+GPS+++LIK++A 
Sbjct: 810 GGAWDNAKKYIETG------ALGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVLIKMLAT 850

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P314140.0e+0079.69Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana ... [more]
P216160.0e+0080.29Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radi... [more]
Q065720.0e+0078.43Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513... [more]
Q72Q292.5e-19756.28Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Q8F6412.5e-19756.28Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Match NameE-valueIdentityDescription
XP_022156348.10.0100.00pyrophosphate-energized vacuolar membrane proton pump 1-like [Momordica charanti... [more]
KAG6586225.10.095.53Pyrophosphate-energized vacuolar membrane proton pump 1, partial [Cucurbita argy... [more]
XP_022938207.10.095.26pyrophosphate-energized vacuolar membrane proton pump 1-like [Cucurbita moschata... [more]
XP_008453661.10.095.53PREDICTED: pyrophosphate-energized vacuolar membrane proton pump 1-like [Cucumis... [more]
XP_022965594.10.095.13pyrophosphate-energized vacuolar membrane proton pump 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DQD80.0100.00H(+)-exporting diphosphatase OS=Momordica charantia OX=3673 GN=LOC111023264 PE=3... [more]
A0A6J1FDE80.095.26H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111444356 PE=3 ... [more]
A0A1S3BXZ50.095.53H(+)-exporting diphosphatase OS=Cucumis melo OX=3656 GN=LOC103494311 PE=3 SV=1[more]
A0A6J1HRF40.095.13H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111465445 PE=3 SV... [more]
A0A0A0LM240.094.87H(+)-exporting diphosphatase OS=Cucumis sativus OX=3659 GN=Csa_2G033950 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G15690.10.0e+0079.69Inorganic H pyrophosphatase family protein [more]
AT1G15690.26.4e-27378.27Inorganic H pyrophosphatase family protein [more]
AT1G78920.11.1e-11839.61vacuolar H+-pyrophosphatase 2 [more]
AT1G78920.21.1e-11839.61vacuolar H+-pyrophosphatase 2 [more]
AT1G16780.14.1e-11839.51Inorganic H pyrophosphatase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004131Pyrophosphate-energised proton pumpPIRSFPIRSF001265H+-PPtasecoord: 22..758
e-value: 1.7E-279
score: 927.3
IPR004131Pyrophosphate-energised proton pumpTIGRFAMTIGR01104TIGR01104coord: 9..755
e-value: 0.0
score: 1169.6
IPR004131Pyrophosphate-energised proton pumpPFAMPF03030H_PPasecoord: 49..745
e-value: 5.2E-257
score: 854.7
IPR004131Pyrophosphate-energised proton pumpPANTHERPTHR31998K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMPcoord: 5..758
IPR004131Pyrophosphate-energised proton pumpHAMAPMF_01129PPase_energized_pumpcoord: 7..750
score: 23.041866
NoneNo IPR availablePANTHERPTHR31998:SF47PYROPHOSPHATE-ENERGIZED VACUOLAR MEMBRANE PROTON PUMP-LIKEcoord: 5..758

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2204.1MC08g2204.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004427 inorganic diphosphatase activity
molecular_function GO:0009678 pyrophosphate hydrolysis-driven proton transmembrane transporter activity