MC08g2201 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2201
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiontranscription termination factor MTERF4, chloroplastic
LocationMC08: 30843353 .. 30844921 (+)
RNA-Seq ExpressionMC08g2201
SyntenyMC08g2201
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAAGACGCCATGCTGGTGTGGTAAAACCTAATTTTTCCATCATAGTACCTAAGATGCCTACTCTTAAATGTTGTAAGTTCACATTTACTACCACATCAATTTTCCAAACGCCTTCTAATCGTGATAGAAGAAGGGGTTTGATTACCCGGCTCCAATACTCTGATGGTGATAGAAGAACAGTATCCAAATCACTGCATTCAACTATTACATCAAAGCCATCCAGCACTAGAGTGGGTAGAAAAGAAGGAAGTGCTTCATCATTATACAGTCACCCTAGCTTGGTGGAGATGAAGAATGTGAGGTTGGAAAATCGTGCAAAGGTTTACGAGTTCTTATGTGGAATTGGTATTGTTCCTGATGAACTTGATGGTTTGGAGCTTCCTGTAACAGTTGATGTTATGAAGGAACGAGTTGATTTTCTTCTCAAATTGGGACTTACAATTGAAGATATAAATAACTACCCACTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGATACCAGTGCTTGATTATCTTGGTAAATTGGGTGTTAGGAAATCTACTTTCACCGAGTTCTTAAGGAGGTATCCACAAGTCCTCCATGCTAGTGTTGTTGTTGACCTTGCTCCAGTAGTTAAGTATCTTCAGGGCATGGATATTAAGCCAAATGATATCCCACACGTTCTTGAGAGATATCCAGAAGTACTGGGCTTCAAGCTTGAGGGAACCATGAGCACTTCAGTGGCATACTTGGTAGGAATTGGAGTGGCAAGAAAAGAAATTGGAGGGGTTTTGACCAAATACCCTGACATTTTAGGAATGAGGGTTGCTCGTGTAATCAAGCCTTTTGTTGAATATCTCGAAAGTTTGGGCATTCCGAGATTAGCAGTGGCTAGGTTAATAGAGAAGCGACCACACATTCTGGGTTTTGGATTGGAAAAAAAGGTCAAACCCAATATCCAAATGCTCTTGAAGTTTAATGTAAGAGAAGAATACCTTTCTTCTATAGTAGCACAGTATGCCGAGATCATAGGAATGGACCTTGAAACTAAGCTTCTAAGTCAAAGGAATTTACTGAAATCGATAATTGAATTGGATGATGATAATTTTGGCACTATTATCGGAAAGATGCCACAAATTGTTAGCCTTAGCAGTTCAGCTGTAATCAACCATGTGGATTTCTTAAAGAGTTGTGGGTTTTCCTTGGTGCAAGTAAAGAACATGGTTATAGGTTGCCCCCAATTGCTTGCTTTGAATCTTGACATAATGAAACACAGCTTCCAGTTCTTCCAAATGGAGATGGGAAGGCCTCTTGATGACTTGGTCACTTTTCCTGCATTCTTTACTTATGGTTTGGAGTCCACAATAAAGCCCAGACATAGGAAGGTTGTGCAGAAGGGAATTACCTGCTCTCTTTCATGGCTCCTCAATTGCTCTGATGAAAAGTTTGCGGAAAGGATGACCTATGACACCATTGATATGGAGGAGATGGAAACTGCACCAACATTCGATATGAATTCTCTTATACAACCCCGGAATGATGATTCAGATTCCGACTATGAAGAGGACAGTGAAGAGGAGACT

mRNA sequence

ATGATAAGACGCCATGCTGGTGTGGTAAAACCTAATTTTTCCATCATAGTACCTAAGATGCCTACTCTTAAATGTTGTAAGTTCACATTTACTACCACATCAATTTTCCAAACGCCTTCTAATCGTGATAGAAGAAGGGGTTTGATTACCCGGCTCCAATACTCTGATGGTGATAGAAGAACAGTATCCAAATCACTGCATTCAACTATTACATCAAAGCCATCCAGCACTAGAGTGGGTAGAAAAGAAGGAAGTGCTTCATCATTATACAGTCACCCTAGCTTGGTGGAGATGAAGAATGTGAGGTTGGAAAATCGTGCAAAGGTTTACGAGTTCTTATGTGGAATTGGTATTGTTCCTGATGAACTTGATGGTTTGGAGCTTCCTGTAACAGTTGATGTTATGAAGGAACGAGTTGATTTTCTTCTCAAATTGGGACTTACAATTGAAGATATAAATAACTACCCACTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGATACCAGTGCTTGATTATCTTGGTAAATTGGGTGTTAGGAAATCTACTTTCACCGAGTTCTTAAGGAGGTATCCACAAGTCCTCCATGCTAGTGTTGTTGTTGACCTTGCTCCAGTAGTTAAGTATCTTCAGGGCATGGATATTAAGCCAAATGATATCCCACACGTTCTTGAGAGATATCCAGAAGTACTGGGCTTCAAGCTTGAGGGAACCATGAGCACTTCAGTGGCATACTTGGTAGGAATTGGAGTGGCAAGAAAAGAAATTGGAGGGGTTTTGACCAAATACCCTGACATTTTAGGAATGAGGGTTGCTCGTGTAATCAAGCCTTTTGTTGAATATCTCGAAAGTTTGGGCATTCCGAGATTAGCAGTGGCTAGGTTAATAGAGAAGCGACCACACATTCTGGGTTTTGGATTGGAAAAAAAGGTCAAACCCAATATCCAAATGCTCTTGAAGTTTAATGTAAGAGAAGAATACCTTTCTTCTATAGTAGCACAGTATGCCGAGATCATAGGAATGGACCTTGAAACTAAGCTTCTAAGTCAAAGGAATTTACTGAAATCGATAATTGAATTGGATGATGATAATTTTGGCACTATTATCGGAAAGATGCCACAAATTGTTAGCCTTAGCAGTTCAGCTGTAATCAACCATGTGGATTTCTTAAAGAGTTGTGGGTTTTCCTTGGTGCAAGTAAAGAACATGGTTATAGGTTGCCCCCAATTGCTTGCTTTGAATCTTGACATAATGAAACACAGCTTCCAGTTCTTCCAAATGGAGATGGGAAGGCCTCTTGATGACTTGGTCACTTTTCCTGCATTCTTTACTTATGGTTTGGAGTCCACAATAAAGCCCAGACATAGGAAGGTTGTGCAGAAGGGAATTACCTGCTCTCTTTCATGGCTCCTCAATTGCTCTGATGAAAAGTTTGCGGAAAGGATGACCTATGACACCATTGATATGGAGGAGATGGAAACTGCACCAACATTCGATATGAATTCTCTTATACAACCCCGGAATGATGATTCAGATTCCGACTATGAAGAGGACAGTGAAGAGGAGACT

Coding sequence (CDS)

ATGATAAGACGCCATGCTGGTGTGGTAAAACCTAATTTTTCCATCATAGTACCTAAGATGCCTACTCTTAAATGTTGTAAGTTCACATTTACTACCACATCAATTTTCCAAACGCCTTCTAATCGTGATAGAAGAAGGGGTTTGATTACCCGGCTCCAATACTCTGATGGTGATAGAAGAACAGTATCCAAATCACTGCATTCAACTATTACATCAAAGCCATCCAGCACTAGAGTGGGTAGAAAAGAAGGAAGTGCTTCATCATTATACAGTCACCCTAGCTTGGTGGAGATGAAGAATGTGAGGTTGGAAAATCGTGCAAAGGTTTACGAGTTCTTATGTGGAATTGGTATTGTTCCTGATGAACTTGATGGTTTGGAGCTTCCTGTAACAGTTGATGTTATGAAGGAACGAGTTGATTTTCTTCTCAAATTGGGACTTACAATTGAAGATATAAATAACTACCCACTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGATACCAGTGCTTGATTATCTTGGTAAATTGGGTGTTAGGAAATCTACTTTCACCGAGTTCTTAAGGAGGTATCCACAAGTCCTCCATGCTAGTGTTGTTGTTGACCTTGCTCCAGTAGTTAAGTATCTTCAGGGCATGGATATTAAGCCAAATGATATCCCACACGTTCTTGAGAGATATCCAGAAGTACTGGGCTTCAAGCTTGAGGGAACCATGAGCACTTCAGTGGCATACTTGGTAGGAATTGGAGTGGCAAGAAAAGAAATTGGAGGGGTTTTGACCAAATACCCTGACATTTTAGGAATGAGGGTTGCTCGTGTAATCAAGCCTTTTGTTGAATATCTCGAAAGTTTGGGCATTCCGAGATTAGCAGTGGCTAGGTTAATAGAGAAGCGACCACACATTCTGGGTTTTGGATTGGAAAAAAAGGTCAAACCCAATATCCAAATGCTCTTGAAGTTTAATGTAAGAGAAGAATACCTTTCTTCTATAGTAGCACAGTATGCCGAGATCATAGGAATGGACCTTGAAACTAAGCTTCTAAGTCAAAGGAATTTACTGAAATCGATAATTGAATTGGATGATGATAATTTTGGCACTATTATCGGAAAGATGCCACAAATTGTTAGCCTTAGCAGTTCAGCTGTAATCAACCATGTGGATTTCTTAAAGAGTTGTGGGTTTTCCTTGGTGCAAGTAAAGAACATGGTTATAGGTTGCCCCCAATTGCTTGCTTTGAATCTTGACATAATGAAACACAGCTTCCAGTTCTTCCAAATGGAGATGGGAAGGCCTCTTGATGACTTGGTCACTTTTCCTGCATTCTTTACTTATGGTTTGGAGTCCACAATAAAGCCCAGACATAGGAAGGTTGTGCAGAAGGGAATTACCTGCTCTCTTTCATGGCTCCTCAATTGCTCTGATGAAAAGTTTGCGGAAAGGATGACCTATGACACCATTGATATGGAGGAGATGGAAACTGCACCAACATTCGATATGAATTCTCTTATACAACCCCGGAATGATGATTCAGATTCCGACTATGAAGAGGACAGTGAAGAGGAGACT

Protein sequence

MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRRTVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET
Homology
BLAST of MC08g2201 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 1.6e-190
Identity = 351/534 (65.73%), Postives = 423/534 (79.21%), Query Frame = 0

Query: 2   IRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRR--RGLITRLQYSDGDR 61
           IR   G  KP F ++  + P+    +    + S +    N  +R   G+   +Q +  +R
Sbjct: 3   IRFCNGFTKPGFLLVHFEPPSFFAVRSRSLSDSTYGNLCNHKKRPGTGIGLTVQCAIANR 62

Query: 62  RTVSKSLHS--TITSKPSSTRVGR-------KEGSASSLYSHPSLVEMKNVRLENRAKVY 121
           R  S+SL S     S  SS+  GR       K   + SLYS PSL++M   +  NRAKVY
Sbjct: 63  RFSSRSLDSPRRERSSRSSSSSGRDRDRDKDKGRDSKSLYSRPSLLDMNKEKAANRAKVY 122

Query: 122 EFLCGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPV 181
           EFL GIGIVPDELDGLELPVT DVMKERV+FL KLGLTIEDINNYPLVLGCSVKKNM+PV
Sbjct: 123 EFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVLGCSVKKNMVPV 182

Query: 182 LDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEV 241
           LDYLGKLGVRKSTFTEFLRRYPQVLH+SVV+DLAPVVKYLQG+DIKP+D+P VLERYPEV
Sbjct: 183 LDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSDVPRVLERYPEV 242

Query: 242 LGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRL 301
           LGFKLEGTMSTSVAYLVGIGVAR+EIGG+LT+YP+ILGMRVAR+IKP VEYLE LGIPRL
Sbjct: 243 LGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLVEYLEVLGIPRL 302

Query: 302 AVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQ 361
           A ARLIEKRPHILGF L+  VKPN+Q+L  FNVRE  L SI+AQY EIIG+DL+ KL +Q
Sbjct: 303 AAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEIIGIDLKPKLDTQ 362

Query: 362 RNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQL 421
           R LL S I L+ ++ G++I +MPQ VSLS S ++ H+DFL  CGFS+ Q + MVIGCPQ+
Sbjct: 363 RKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQTREMVIGCPQV 422

Query: 422 LALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWL 481
           LALNL IMK SF++FQ EM RPL DLV FPAFFTYGLEST+KPRH+K+++KGI CSL+W+
Sbjct: 423 LALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKIIKKGIKCSLAWM 482

Query: 482 LNCSDEKFAERMTYDTIDMEEMETAP-TFDMNSLIQP-RNDDSDSDYEEDSEEE 523
           LNCSDEKF +RM+YDTID+EE+ET P +FDMN+L+QP R ++SDS+YEE+ +++
Sbjct: 483 LNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEEEEDDD 536

BLAST of MC08g2201 vs. ExPASy Swiss-Prot
Match: Q6AUK6 (Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0404000 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 8.4e-160
Identity = 284/470 (60.43%), Postives = 362/470 (77.02%), Query Frame = 0

Query: 68  STITSKPSSTRVGRKEGSA-------------SSLYSHPSLVEMKNVRLENRAKVYEFLC 127
           S  TS  S  R G   G A             SSLY+ PSL++M+  R   RA V  FL 
Sbjct: 32  SASTSASSPPRAGCSRGPAHARPRPSPRPSPSSSLYARPSLLDMERGRAARRADVDAFLA 91

Query: 128 GIGIVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYL 187
            +G+ P EL GLELP TVDVM+ERV+FL  L L+ ED+  YPL LGCSV+KNM+PVLDYL
Sbjct: 92  SLGVDPGELAGLELPATVDVMRERVEFLHSLDLSNEDLAAYPLALGCSVRKNMVPVLDYL 151

Query: 188 GKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFK 247
           GKLGVR+    + LRRYPQVLHASVVVDLAPVVKYLQGMD++P+D+P VLERYPE+LGFK
Sbjct: 152 GKLGVRQDALPDLLRRYPQVLHASVVVDLAPVVKYLQGMDVRPHDVPRVLERYPELLGFK 211

Query: 248 LEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVAR 307
           LEGTMSTS+AYLVGIGVAR+++G V+T++P++LGMRV ++IKPFVE+LE +G+ RLA+AR
Sbjct: 212 LEGTMSTSIAYLVGIGVARRQVGSVITRFPEVLGMRVGKIIKPFVEHLEGIGLQRLAIAR 271

Query: 308 LIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLL 367
           +IEK+P++LGFGLE KVKPNI+ LL+F VR+E L+ IVAQY +I+G++L  KL +Q++L 
Sbjct: 272 IIEKKPYVLGFGLEDKVKPNIEALLEFGVRKEALAFIVAQYPDILGIELRDKLATQQSLF 331

Query: 368 KSIIELDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALN 427
           +S I +  ++FG +I +MPQ +SL  +AV+ HV+FL SCGF L QV  MV+ CPQLLALN
Sbjct: 332 ESSILVSSEDFGRVIERMPQAISLGRTAVLKHVNFLTSCGFLLSQVSKMVVACPQLLALN 391

Query: 428 LDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCS 487
           +DIMK SF++FQ EM R L++LV FPAFFTYGLEST++PRH  V +KG TCSL+WLLNCS
Sbjct: 392 MDIMKMSFEYFQNEMERDLEELVEFPAFFTYGLESTVRPRHEMVAKKGFTCSLAWLLNCS 451

Query: 488 DEKFAERMTYDTIDMEEMETAPTFDMNSLIQPRNDD-SDSDYEEDSEEET 524
           D KF ERM YDTI +EEME   +FD N+L +   D+  D D +EDS+ ++
Sbjct: 452 DAKFDERMKYDTIGIEEMEVDNSFDTNTLSERVEDEVEDEDLDEDSDYDS 501

BLAST of MC08g2201 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.5e-156
Identity = 266/438 (60.73%), Postives = 351/438 (80.14%), Query Frame = 0

Query: 86  ASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVPDELDGLELPVTVDVMKERVDFLLKL 145
           +SSLY+ PSL++M+  R   RA V  FL  +G+ P EL GLELPVTVDVM+ER +FL  L
Sbjct: 54  SSSLYARPSLLDMERDRATRRADVDAFLTSLGVDPGELAGLELPVTVDVMRERAEFLGSL 113

Query: 146 GLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAP 205
           GLT ED+  YPL LGCSV+KNM+PVLDYLGKLGVR+    + LRRYPQVLHASVVVDLAP
Sbjct: 114 GLTQEDLAAYPLALGCSVRKNMVPVLDYLGKLGVRRDALPDLLRRYPQVLHASVVVDLAP 173

Query: 206 VVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPD 265
           VVKYLQGMD++P D+P VLERYPE+LGFKLEGTMSTSVAYLVGIGV R+++G V+T++P+
Sbjct: 174 VVKYLQGMDVRPTDVPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFPE 233

Query: 266 ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVRE 325
           +LGMRV ++IKPFVE+LE +G+ RLA+AR+IEK+P++LGFGL++KVKPNI+ L+   VR+
Sbjct: 234 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVRK 293

Query: 326 EYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVIN 385
           E L+SIV QY +++G++L  KL++Q++L +S I +  ++FG ++ +MPQ +SL  +AV+ 
Sbjct: 294 EALASIVMQYPDVLGLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAISLGRAAVLK 353

Query: 386 HVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTY 445
           HV+FL +CGF L QV  MV+ CPQLLALN+DIM+ +F++F+ EM R L++LV FPAFFTY
Sbjct: 354 HVNFLTACGFMLSQVSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPAFFTY 413

Query: 446 GLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPTFDMNSLIQ 505
           G+EST++PRH  V +KG+TCSL+WLLNCSD KF ERM YDTI +EEME   + DMN+ + 
Sbjct: 414 GIESTVRPRHEMVSRKGLTCSLAWLLNCSDAKFDERMKYDTIGVEEMEREDSSDMNASVD 473

Query: 506 PRNDD--SDSDYEEDSEE 522
               +   DSDY +  +E
Sbjct: 474 EVESEEYEDSDYGDSDDE 491

BLAST of MC08g2201 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.2e-34
Identity = 93/361 (25.76%), Postives = 179/361 (49.58%), Query Frame = 0

Query: 164 KKNMIPVLDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHV 223
           ++ +  +++YL   GVR+      + R P++L  S + ++   V +   M +  ND   +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 224 LERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLE 283
           +  YP+++GF     M   + YL   G++ +E+G +L   P ++G  +    KP V+Y  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 284 SLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDL 343
            LGIP+  + R++  +P +    LEK + P ++ L +  +  E + +++ ++  ++   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 344 ETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVIN-HVDFLKSCGFSLVQVKN 403
             K+      L +   +   + G +I   P ++  S    +  ++ +  S G    Q+  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 404 MVIGCPQLLALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKG 463
           M+   P LL  N+D ++  +++ +  M RPL DL+ FP FF+Y LE  I PRH  +V+  
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 464 ITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPTFDMNSLIQPRN-DDSDSDYEEDSEE 523
           +   L ++L C+DE+F ER   D ++  E   A     +S     N  D +  + +++EE
Sbjct: 578 VNFKLRYMLACTDEEF-ERRVRDKVERRERFEAGLDSEDSQPSDENISDQEIAFSDEAEE 636

BLAST of MC08g2201 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.3e-31
Identity = 67/276 (24.28%), Postives = 148/276 (53.62%), Query Frame = 0

Query: 215 IKPNDIPHVLERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARV 274
           I+   +P+++ R P++L  +L+  +   V  L  +G   +E+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 275 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREE-YLSSIVA 334
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 335 QYAEIIGMDLETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVIN-HVDFLKS 394
           +   ++G  ++ +L      LKS + L +D   +++   PQ++    + ++  + D+LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 395 CGFSLVQVKNMVIGCPQLLALNL-DIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTI 454
           CGF   Q+  MV G PQ+L  ++ + ++   +F    MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 455 KPRHRKVVQKGITCSLSWLLNCSDEKFAERMTYDTI 488
           + R + V +  I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

BLAST of MC08g2201 vs. NCBI nr
Match: XP_022156431.1 (transcription termination factor MTERF4, chloroplastic [Momordica charantia])

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR
Sbjct: 2   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP
Sbjct: 62  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL
Sbjct: 302 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET
Sbjct: 482 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 524

BLAST of MC08g2201 vs. NCBI nr
Match: XP_023538106.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023538107.1 transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 474/523 (90.63%), Postives = 496/523 (94.84%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR H GVVKPNFSI++ +MPTLKC KF F TTS FQT  N ++RRGLIT+LQYS GDRR
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPTLKCQKFKFITTSTFQTSPNHEKRRGLITKLQYSRGDRR 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKP S++V RKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIVP
Sbjct: 62  TVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEK+VKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIEL
Sbjct: 302 HILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           D+DNFGTII KMPQ+VSLSSSAV +HVDFLKSCGFSL QVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFFQ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVVQKG+TCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDM+SL QPR D+SDSDYEEDSEEET
Sbjct: 482 RMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of MC08g2201 vs. NCBI nr
Match: KAG7021053.1 (Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 472/523 (90.25%), Postives = 497/523 (95.03%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR H GV+KPNFSI++ +MPTLKC KF F TTS FQT  N ++RRGLIT+LQYS GDRR
Sbjct: 15  MIRGHTGVLKPNFSIMLLEMPTLKCHKFKFGTTSTFQTSPNHEKRRGLITKLQYSRGDRR 74

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKP S++VGRKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIVP
Sbjct: 75  TVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVP 134

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGVR
Sbjct: 135 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 194

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTMS
Sbjct: 195 KSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMS 254

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 255 TSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 314

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEK+VKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIEL
Sbjct: 315 HILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIEL 374

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           D++NFGTII KMPQ+VSLSSSAV +HVDFLKSCGFSL QVKNMVIGCPQLLALNLDIMKH
Sbjct: 375 DNNNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKH 434

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFF+ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVVQKG+TCSLSWLLNCSDEKFAE
Sbjct: 435 SFQFFRKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFAE 494

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDM+SL QPR D+SDSDYEEDSEEET
Sbjct: 495 RMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 537

BLAST of MC08g2201 vs. NCBI nr
Match: KAG6586221.1 (Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 915 bits (2366), Expect = 0.0
Identity = 472/523 (90.25%), Postives = 497/523 (95.03%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR H GV+KPNFSI++ +MPTLKC KF F TTS FQT  N ++RRGLIT+LQYS GDRR
Sbjct: 2   MIRGHTGVLKPNFSIMLLEMPTLKCHKFKFGTTSTFQTSPNHEKRRGLITKLQYSRGDRR 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKP S++VGRKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIVP
Sbjct: 62  TVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEK+VKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIEL
Sbjct: 302 HILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           D++NFGTII KMPQ+VSLSSSAV +HVDFLKSCGFSL QVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DNNNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFF+ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVVQKG+TCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFRKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGMTCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDM+SL QPR D+SDSDYEEDSEEET
Sbjct: 482 RMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of MC08g2201 vs. NCBI nr
Match: XP_022938209.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_022938210.1 transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_022938211.1 transcription termination factor MTERF4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 473/523 (90.44%), Postives = 495/523 (94.65%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR H GVVKPNFSI++ +MPTLKC KF F TTS FQT  N ++RRGLIT+LQYS GD R
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPTLKCHKFKFDTTSTFQTSPNHEKRRGLITKLQYSRGDWR 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKP S++VGRKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIVP
Sbjct: 62  TVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEK+VKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIEL
Sbjct: 302 HILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           D+DNFGTII KMPQ+VSLSSSAV +HVD LKSCGFSL QVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DNDNFGTIIEKMPQVVSLSSSAVNSHVDCLKSCGFSLQQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFFQ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVVQKG+TCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDM+SL QPR D+SDSDYEEDSEEET
Sbjct: 482 RMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of MC08g2201 vs. ExPASy TrEMBL
Match: A0A6J1DUY1 (transcription termination factor MTERF4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023323 PE=3 SV=1)

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR
Sbjct: 2   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP
Sbjct: 62  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL
Sbjct: 302 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET
Sbjct: 482 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 524

BLAST of MC08g2201 vs. ExPASy TrEMBL
Match: A0A6J1FDE7 (transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111444359 PE=3 SV=1)

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 473/523 (90.44%), Postives = 495/523 (94.65%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR H GVVKPNFSI++ +MPTLKC KF F TTS FQT  N ++RRGLIT+LQYS GD R
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPTLKCHKFKFDTTSTFQTSPNHEKRRGLITKLQYSRGDWR 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKP S++VGRKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIVP
Sbjct: 62  TVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNM+PVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEK+VKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIEL
Sbjct: 302 HILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           D+DNFGTII KMPQ+VSLSSSAV +HVD LKSCGFSL QVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DNDNFGTIIEKMPQVVSLSSSAVNSHVDCLKSCGFSLQQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFFQ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVVQKG+TCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAPTFDM+SL QPR D+SDSDYEEDSEEET
Sbjct: 482 RMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of MC08g2201 vs. ExPASy TrEMBL
Match: A0A6J1HPD6 (transcription termination factor MTERF4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111465502 PE=3 SV=1)

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 468/523 (89.48%), Postives = 490/523 (93.69%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR H GVVKPNFSI++ +MPTLKC KF F TTS FQ   N ++R  LIT+LQYS GDR+
Sbjct: 2   MIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDRK 61

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIVP 120
           TVSKSLHSTITSKP S++V RKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIVP
Sbjct: 62  TVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVP 121

Query: 121 DELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGVR 180
           DELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNM+PVLDYLGKLGVR
Sbjct: 122 DELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVR 181

Query: 181 KSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTMS 240
           KSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTMS
Sbjct: 182 KSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMS 241

Query: 241 TSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 300
           TSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP
Sbjct: 242 TSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRP 301

Query: 301 HILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIEL 360
           HILGFGLEKKVKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIEL
Sbjct: 302 HILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIEL 361

Query: 361 DDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMKH 420
           D+DNFGTII KMPQ+VSLSSSAV +HVDFLKSCGFSL QVKNMVIGCPQLLALNLDIMKH
Sbjct: 362 DNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKH 421

Query: 421 SFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFAE 480
           SFQFFQ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVV KGITCSLSWLLNCSDEKFAE
Sbjct: 422 SFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFAE 481

Query: 481 RMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           RMTYDTIDMEEMETAP+FDM+SL QPR D+SDSDYEED EEET
Sbjct: 482 RMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 524

BLAST of MC08g2201 vs. ExPASy TrEMBL
Match: A0A0A0KRV7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G466880 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 488/524 (93.13%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR HAGV KPNFS+++ +M TL+C  F FT T  FQ P N ++R+GLIT+LQYS GDRR
Sbjct: 1   MIRGHAGVAKPNFSLMLFEMSTLECHNFKFTATRTFQMPYNHEKRKGLITQLQYSHGDRR 60

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGS-ASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIV 120
           TV KSLHSTI+SK  S++V RKEGS  SSLYS PSLVEMKNVRLENR+KVYEFL GIG+V
Sbjct: 61  TVFKSLHSTISSKSYSSKVDRKEGSIGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPL+LGCSVKKNMIPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLILGCSVKKNMIPVLDYLGKLGV 180

Query: 181 RKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTM 240
           RKSTFTEFL+RYPQVLH+SVVVDLAPV+KYLQGMDIKPNDIP VLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPNDIPRVLERYPEVLGFKLEGTM 240

Query: 241 STSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGV R+EIGGVLTKYP+ILGMRVARVIKPFVEYLE LGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVTRREIGGVLTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEKR 300

Query: 301 PHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIE 360
           PHILGFGLEKKVKPNIQ+LL+F VRE YL SIVAQYAEIIG+DLE  L +QRNLLKS+IE
Sbjct: 301 PHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGIDLEANLQTQRNLLKSLIE 360

Query: 361 LDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMK 420
           LD+DNFGTII KMPQ+VSLS SAVINHVDFLK+CGFSL+QVKNMVIGCPQLLALN+DIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIMK 420

Query: 421 HSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFA 480
           HSF+FFQMEMGRPL+DL TFPAFFTYGLESTIKPRHRKVVQKGI+CSLSWLLNC+DEKF 
Sbjct: 421 HSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKFM 480

Query: 481 ERMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           ERM+YDTIDMEEMET PTFDMNSLIQPRND+SDS+YEEDSEEE+
Sbjct: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDSEEES 524

BLAST of MC08g2201 vs. ExPASy TrEMBL
Match: A0A1S3CR33 (transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503367 PE=3 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 452/524 (86.26%), Postives = 486/524 (92.75%), Query Frame = 0

Query: 1   MIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDRR 60
           MIR HAGVVKPN S ++ +M TL+C  F FT T  F T    ++RR L T+LQYS GDRR
Sbjct: 1   MIRGHAGVVKPNVSFMLFEMSTLECHNFKFTATPTFLTSCIHEKRRCLTTQLQYSHGDRR 60

Query: 61  TVSKSLHSTITSKPSSTRVGRKEGSA-SSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIV 120
           TV KSLHSTI+SKP S++VGRK GS+ SSLYS PSLVEMKNVRLENR+KVYEFL GIG+V
Sbjct: 61  TVFKSLHSTISSKPYSSKVGRKGGSSGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120

Query: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180
           PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180

Query: 181 RKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTM 240
           RKSTFTEFL+RYPQVLH+SVVVDLAPVVKYLQGMDIKPNDIP VLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVVKYLQGMDIKPNDIPRVLERYPEVLGFKLEGTM 240

Query: 241 STSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300
           STSVAYLVGIGVAR+EIGG+LTKYP+ILGMRVARVIKPFVEYLE LGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGILTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEKR 300

Query: 301 PHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIE 360
           PHILGFGLEKKVKPNIQ+LL+F VRE YL SIVAQYAEIIGMDLE  L +QRNLLKS+IE
Sbjct: 301 PHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGMDLEANLQTQRNLLKSLIE 360

Query: 361 LDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMK 420
           LD+DNFGTII KMPQIVSLS SAVINHVDFLK+CGFSL+QVKNMVIGCPQLLALN+DIMK
Sbjct: 361 LDNDNFGTIIEKMPQIVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIMK 420

Query: 421 HSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFA 480
           HSF+FFQMEMGRPL+DL TFPAFFTYGLESTIKPRHRKVVQKGI+CSLSWLLNC+DEKF 
Sbjct: 421 HSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKFV 480

Query: 481 ERMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 523
           ERM+YDTIDMEEMET PTFDMNSL+QPRND+SDS+YEEDSEEET
Sbjct: 481 ERMSYDTIDMEEMETTPTFDMNSLVQPRNDESDSEYEEDSEEET 524

BLAST of MC08g2201 vs. TAIR 10
Match: AT4G02990.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 667.2 bits (1720), Expect = 1.1e-191
Identity = 351/534 (65.73%), Postives = 423/534 (79.21%), Query Frame = 0

Query: 2   IRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRR--RGLITRLQYSDGDR 61
           IR   G  KP F ++  + P+    +    + S +    N  +R   G+   +Q +  +R
Sbjct: 3   IRFCNGFTKPGFLLVHFEPPSFFAVRSRSLSDSTYGNLCNHKKRPGTGIGLTVQCAIANR 62

Query: 62  RTVSKSLHS--TITSKPSSTRVGR-------KEGSASSLYSHPSLVEMKNVRLENRAKVY 121
           R  S+SL S     S  SS+  GR       K   + SLYS PSL++M   +  NRAKVY
Sbjct: 63  RFSSRSLDSPRRERSSRSSSSSGRDRDRDKDKGRDSKSLYSRPSLLDMNKEKAANRAKVY 122

Query: 122 EFLCGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPV 181
           EFL GIGIVPDELDGLELPVT DVMKERV+FL KLGLTIEDINNYPLVLGCSVKKNM+PV
Sbjct: 123 EFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVLGCSVKKNMVPV 182

Query: 182 LDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEV 241
           LDYLGKLGVRKSTFTEFLRRYPQVLH+SVV+DLAPVVKYLQG+DIKP+D+P VLERYPEV
Sbjct: 183 LDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSDVPRVLERYPEV 242

Query: 242 LGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRL 301
           LGFKLEGTMSTSVAYLVGIGVAR+EIGG+LT+YP+ILGMRVAR+IKP VEYLE LGIPRL
Sbjct: 243 LGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLVEYLEVLGIPRL 302

Query: 302 AVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQ 361
           A ARLIEKRPHILGF L+  VKPN+Q+L  FNVRE  L SI+AQY EIIG+DL+ KL +Q
Sbjct: 303 AAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEIIGIDLKPKLDTQ 362

Query: 362 RNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQL 421
           R LL S I L+ ++ G++I +MPQ VSLS S ++ H+DFL  CGFS+ Q + MVIGCPQ+
Sbjct: 363 RKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQTREMVIGCPQV 422

Query: 422 LALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWL 481
           LALNL IMK SF++FQ EM RPL DLV FPAFFTYGLEST+KPRH+K+++KGI CSL+W+
Sbjct: 423 LALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKIIKKGIKCSLAWM 482

Query: 482 LNCSDEKFAERMTYDTIDMEEMETAP-TFDMNSLIQP-RNDDSDSDYEEDSEEE 523
           LNCSDEKF +RM+YDTID+EE+ET P +FDMN+L+QP R ++SDS+YEE+ +++
Sbjct: 483 LNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEEEEDDD 536

BLAST of MC08g2201 vs. TAIR 10
Match: AT2G44020.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 446.8 bits (1148), Expect = 2.4e-125
Identity = 211/428 (49.30%), Postives = 317/428 (74.07%), Query Frame = 0

Query: 95  LVEMKNVRLENRAKVYEFLCGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINN 154
           +++ K  +L NR K+ ++L G+GI+ DEL+ +ELP T++VM ERV+FL KLGLTI+DIN 
Sbjct: 72  VIQGKKEKLVNRVKICDYLKGLGIITDELESIELPSTIEVMCERVEFLQKLGLTIDDINE 131

Query: 155 YPLVLGCSVKKNMIPVLDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMD 214
           YPL+LGCSV+KN+IPVL YL K+G+ +S   EF++ YPQVLHASVVV+LAPVVK+L+G+D
Sbjct: 132 YPLMLGCSVRKNLIPVLAYLEKIGISRSKLGEFVKNYPQVLHASVVVELAPVVKFLRGLD 191

Query: 215 IKPNDIPHVLERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARV 274
           ++  D+ +VL +YPE+LGFKLEGTMSTSVAYLV IGV+ ++IG ++T+YP +LGMRV  +
Sbjct: 192 VEKQDLGYVLMKYPELLGFKLEGTMSTSVAYLVSIGVSPRDIGPMVTQYPYLLGMRVGTM 251

Query: 275 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQ 334
           IKP V+YL S+G+P+  VAR++EKR +I+G+ LE+ VKPN+  L+ F V++E L  ++AQ
Sbjct: 252 IKPLVDYLISIGLPKKIVARMLEKRSYIVGYNLEETVKPNVDCLISFGVKKELLPLLIAQ 311

Query: 335 YAEIIGMDLETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCG 394
           Y +I+G+ ++ K+ +Q+      +++D + F  ++ KMPQIVSL  + ++  ++FL    
Sbjct: 312 YPQILGLPVKAKMSTQQYFFSLKLKIDPEGFARVVEKMPQIVSLKQNVIMKPIEFLLGRA 371

Query: 395 FSLVQVKNMVIGCPQLLALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPR 454
           F +  +  MV+ CPQ+L   +++MK+S+ F++ EMGRP+ +LV +P +FTY LES IKPR
Sbjct: 372 FQVEDIAKMVVRCPQILCSRVELMKNSYYFYKTEMGRPMKELVEYPEYFTYSLESRIKPR 431

Query: 455 HRKVVQKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSD 514
           ++K+  KGI  SL+W LNCSD++F ER+  + ID +     PTFDM   ++    +  +D
Sbjct: 432 YQKLQSKGIRSSLNWFLNCSDQRFEERLQGNFIDPD--TEGPTFDMGGKLEMPGGEIVTD 491

Query: 515 YEEDSEEE 523
            EED  ++
Sbjct: 492 EEEDESDD 497

BLAST of MC08g2201 vs. TAIR 10
Match: AT2G21710.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 147.5 bits (371), Expect = 3.0e-35
Identity = 93/361 (25.76%), Postives = 179/361 (49.58%), Query Frame = 0

Query: 164 KKNMIPVLDYLGKLGVRKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHV 223
           ++ +  +++YL   GVR+      + R P++L  S + ++   V +   M +  ND   +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 224 LERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLE 283
           +  YP+++GF     M   + YL   G++ +E+G +L   P ++G  +    KP V+Y  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 284 SLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDL 343
            LGIP+  + R++  +P +    LEK + P ++ L +  +  E + +++ ++  ++   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 344 ETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVIN-HVDFLKSCGFSLVQVKN 403
             K+      L +   +   + G +I   P ++  S    +  ++ +  S G    Q+  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 404 MVIGCPQLLALNLDIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKG 463
           M+   P LL  N+D ++  +++ +  M RPL DL+ FP FF+Y LE  I PRH  +V+  
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 464 ITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPTFDMNSLIQPRN-DDSDSDYEEDSEE 523
           +   L ++L C+DE+F ER   D ++  E   A     +S     N  D +  + +++EE
Sbjct: 578 VNFKLRYMLACTDEEF-ERRVRDKVERRERFEAGLDSEDSQPSDENISDQEIAFSDEAEE 636

BLAST of MC08g2201 vs. TAIR 10
Match: AT4G38160.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 137.9 bits (346), Expect = 2.4e-32
Identity = 67/276 (24.28%), Postives = 148/276 (53.62%), Query Frame = 0

Query: 215 IKPNDIPHVLERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARV 274
           I+   +P+++ R P++L  +L+  +   V  L  +G   +E+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 275 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREE-YLSSIVA 334
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 335 QYAEIIGMDLETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVIN-HVDFLKS 394
           +   ++G  ++ +L      LKS + L +D   +++   PQ++    + ++  + D+LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 395 CGFSLVQVKNMVIGCPQLLALNL-DIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTI 454
           CGF   Q+  MV G PQ+L  ++ + ++   +F    MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 455 KPRHRKVVQKGITCSLSWLLNCSDEKFAERMTYDTI 488
           + R + V +  I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

BLAST of MC08g2201 vs. TAIR 10
Match: AT4G38160.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 137.9 bits (346), Expect = 2.4e-32
Identity = 67/276 (24.28%), Postives = 148/276 (53.62%), Query Frame = 0

Query: 215 IKPNDIPHVLERYPEVLGFKLEGTMSTSVAYLVGIGVARKEIGGVLTKYPDILGMRVARV 274
           I+   +P+++ R P++L  +L+  +   V  L  +G   +E+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 275 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQMLLKFNVREE-YLSSIVA 334
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 335 QYAEIIGMDLETKLLSQRNLLKSIIELDDDNFGTIIGKMPQIVSLSSSAVIN-HVDFLKS 394
           +   ++G  ++ +L      LKS + L +D   +++   PQ++    + ++  + D+LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 395 CGFSLVQVKNMVIGCPQLLALNL-DIMKHSFQFFQMEMGRPLDDLVTFPAFFTYGLESTI 454
           CGF   Q+  MV G PQ+L  ++ + ++   +F    MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 455 KPRHRKVVQKGITCSLSWLLNCSDEKFAERMTYDTI 488
           + R + V +  I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZT961.6e-19065.73Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q6AUK68.4e-16060.43Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. ja... [more]
B6TGN41.5e-15660.73Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
F4IHL34.2e-3425.76Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL63.3e-3124.28Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
XP_022156431.10.0100.00transcription termination factor MTERF4, chloroplastic [Momordica charantia][more]
XP_023538106.10.090.63transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pe... [more]
KAG7021053.10.090.25Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyr... [more]
KAG6586221.10.090.25Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyr... [more]
XP_022938209.10.090.44transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_... [more]
Match NameE-valueIdentityDescription
A0A6J1DUY10.0100.00transcription termination factor MTERF4, chloroplastic OS=Momordica charantia OX... [more]
A0A6J1FDE70.090.44transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=... [more]
A0A6J1HPD60.089.48transcription termination factor MTERF4, chloroplastic OS=Cucurbita maxima OX=36... [more]
A0A0A0KRV70.085.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G466880 PE=3 SV=1[more]
A0A1S3CR330.086.26transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT4G02990.11.1e-19165.73Mitochondrial transcription termination factor family protein [more]
AT2G44020.12.4e-12549.30Mitochondrial transcription termination factor family protein [more]
AT2G21710.13.0e-3525.76Mitochondrial transcription termination factor family protein [more]
AT4G38160.12.4e-3224.28Mitochondrial transcription termination factor family protein [more]
AT4G38160.22.4e-3224.28Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 86..119
e-value: 410.0
score: 6.9
coord: 401..432
e-value: 0.11
score: 21.6
coord: 257..288
e-value: 6.2E-4
score: 29.1
coord: 221..252
e-value: 0.011
score: 24.9
coord: 293..324
e-value: 7.3E-4
score: 28.8
coord: 433..464
e-value: 0.073
score: 22.2
coord: 366..396
e-value: 0.022
score: 23.9
coord: 185..216
e-value: 0.0016
score: 27.7
coord: 329..361
e-value: 160.0
score: 10.0
coord: 149..180
e-value: 0.0039
score: 26.4
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 171..479
e-value: 5.9E-104
score: 347.4
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 31..512
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 134..274
e-value: 6.1E-25
score: 89.8
coord: 275..488
e-value: 2.3E-48
score: 166.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 498..523
NoneNo IPR availablePANTHERPTHR13068:SF109TRANSCRIPTION TERMINATION FACTOR MTERF4, CHLOROPLASTICcoord: 31..512

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2201.1MC08g2201.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0042794 plastid rRNA transcription
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0008380 RNA splicing
cellular_component GO:0009536 plastid
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0003729 mRNA binding