MC08g2198 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2198
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	nicotianamine synthase
Location	MC08: 30820127 .. 30820945 (-)
RNA-Seq Expression	MC08g2198
Synteny	MC08g2198

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTGCCAAGAAGTGCTTTTGGTCCAAAAAGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCCCAACTCGTCCTCACCTGCGCCCCACCGCCACCATCACCGCCACCCATCGACGTCTCGAAGCTCGGCTCCGCCGTCCAAAAAATGAGGTCCCACCTCATCAAGCTCTGCGGCGAGGCCGAGGCCCTCCTTGAGCACCATTACTCCTCCATTTTAGCTTCCCATGAAAACCCTCTTGAAAATCTCTCCATTTTCCCTTACTACTCCAACTACTTGAAACTTGCACACCTTGAATTCTCCATTTTGGAACGCCACGCGCAGCCGTCCAAGGTGGCTTTCGTTGGCTCTGGGGCGCTCCCGTTCACGTCGATCATCATGGCCTCCAAGTACCTCCCGGCCACCGAGTTCCACAACTTCGACGTCGACCCGTCAGCCAACGCGAAGGCTGCGGCCCTGGTGGCCGGGGACCGGGACCTGGAGCGGCGGATGGTGTTCCACACGGCGGACATCATGGAGGTGACCGGCGAGCTGAGGGGGTTCCAAGTGGTATTTTTGGCGGCGTTGGTGGGGATGGAGCGGGAGGAGAAGGTGAAGGTGATCGAGCACTTGGGGAAAAACATGGGCGGCGGCGCGTTGCTGATGCTGAGGAGCGCGTATGGAGCACGCGCCTTCCTTTATCCAGTTGTGGAAGCTCGTGATCTCCAAGGGTTTGAGATTCTTACAGTGTTTCATCCCACGGACGAGGTTATCAATTCTGTTGTGTTGGCTCGATTG

mRNA sequence

ATGTGTTGCCAAGAAGTGCTTTTGGTCCAAAAAGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCCCAACTCGTCCTCACCTGCGCCCCACCGCCACCATCACCGCCACCCATCGACGTCTCGAAGCTCGGCTCCGCCGTCCAAAAAATGAGGTCCCACCTCATCAAGCTCTGCGGCGAGGCCGAGGCCCTCCTTGAGCACCATTACTCCTCCATTTTAGCTTCCCATGAAAACCCTCTTGAAAATCTCTCCATTTTCCCTTACTACTCCAACTACTTGAAACTTGCACACCTTGAATTCTCCATTTTGGAACGCCACGCGCAGCCGTCCAAGGTGGCTTTCGTTGGCTCTGGGGCGCTCCCGTTCACGTCGATCATCATGGCCTCCAAGTACCTCCCGGCCACCGAGTTCCACAACTTCGACGTCGACCCGTCAGCCAACGCGAAGGCTGCGGCCCTGGTGGCCGGGGACCGGGACCTGGAGCGGCGGATGGTGTTCCACACGGCGGACATCATGGAGGTGACCGGCGAGCTGAGGGGGTTCCAAGTGGTATTTTTGGCGGCGTTGGTGGGGATGGAGCGGGAGGAGAAGGTGAAGGTGATCGAGCACTTGGGGAAAAACATGGGCGGCGGCGCGTTGCTGATGCTGAGGAGCGCGTATGGAGCACGCGCCTTCCTTTATCCAGTTGTGGAAGCTCGTGATCTCCAAGGGTTTGAGATTCTTACAGTGTTTCATCCCACGGACGAGGTTATCAATTCTGTTGTGTTGGCTCGATTG

Coding sequence (CDS)

ATGTGTTGCCAAGAAGTGCTTTTGGTCCAAAAAGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCCCAACTCGTCCTCACCTGCGCCCCACCGCCACCATCACCGCCACCCATCGACGTCTCGAAGCTCGGCTCCGCCGTCCAAAAAATGAGGTCCCACCTCATCAAGCTCTGCGGCGAGGCCGAGGCCCTCCTTGAGCACCATTACTCCTCCATTTTAGCTTCCCATGAAAACCCTCTTGAAAATCTCTCCATTTTCCCTTACTACTCCAACTACTTGAAACTTGCACACCTTGAATTCTCCATTTTGGAACGCCACGCGCAGCCGTCCAAGGTGGCTTTCGTTGGCTCTGGGGCGCTCCCGTTCACGTCGATCATCATGGCCTCCAAGTACCTCCCGGCCACCGAGTTCCACAACTTCGACGTCGACCCGTCAGCCAACGCGAAGGCTGCGGCCCTGGTGGCCGGGGACCGGGACCTGGAGCGGCGGATGGTGTTCCACACGGCGGACATCATGGAGGTGACCGGCGAGCTGAGGGGGTTCCAAGTGGTATTTTTGGCGGCGTTGGTGGGGATGGAGCGGGAGGAGAAGGTGAAGGTGATCGAGCACTTGGGGAAAAACATGGGCGGCGGCGCGTTGCTGATGCTGAGGAGCGCGTATGGAGCACGCGCCTTCCTTTATCCAGTTGTGGAAGCTCGTGATCTCCAAGGGTTTGAGATTCTTACAGTGTTTCATCCCACGGACGAGGTTATCAATTCTGTTGTGTTGGCTCGATTG

Protein sequence

MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERHAQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL

Homology

[Close]

BLAST of MC08g2198 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 1.9e-91
Identity = 170/274 (62.04%), Postives = 219/274 (79.93%), Query Frame = 0


Query: 3   CQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSAVQ 62
           CQ+  LV K+ +LY KIS L++LKP +DV++LF QLV TC PP P+   IDV+K+   +Q
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN---IDVTKMSENIQ 63

Query: 63  KMRSHLIKLCGEAEALLEHHYSSILASHE-NPLENLSIFPYYSNYLKLAHLEFSILERHA 122
           +MRS+LIK+CGEAE  LEHH+SSIL S E NPL +L++FPYY+NYLKL+ LEF +LE++ 
Sbjct: 64  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 123

Query: 123 Q---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRM 182
               P  VAF+GSG LP TS+++AS +L  + FHNFD+DPSAN  AA LV+ D DL +RM
Sbjct: 124 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 183

Query: 183 VFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARA 242
            FHT DIM+VT  L+GF VVFLAALVGM+++EKVKV+EHL K+M  GALLMLRSA+G RA
Sbjct: 184 FFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRA 243

Query: 243 FLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           FLYP+VE  DL+GFE+L+V+HPTDEVINS+V++R
Sbjct: 244 FLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of MC08g2198 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.1e-89
Identity = 165/274 (60.22%), Postives = 217/274 (79.20%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           +C     +V+KV ELY +IS L++L PSKDVN LF+ LV TC P    P PIDVSKL   
Sbjct: 2   VCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMP----PNPIDVSKLCQK 61

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120
           +Q++RSHLIKLCG+AE LLE H+S IL+S+ENPL++L IFPY+ NY+KL+ LE++IL ++
Sbjct: 62  IQEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKN 121

Query: 121 AQ--PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRM 180
               P K+AF+GSG LP TS+++A+K+L  T FHN+D+D  AN  A+ALVA D D+  RM
Sbjct: 122 TTNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRM 181

Query: 181 VFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARA 240
            FHTAD+M+VT  L+ + VVFLAALVGM++E+KVKV++HL K M  GA LMLRSA+GARA
Sbjct: 182 TFHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARA 241

Query: 241 FLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           FLYPV++ RDL+GFE+L+V+HPTDEVINSV++AR
Sbjct: 242 FLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of MC08g2198 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.2e-88
Identity = 170/276 (61.59%), Postives = 217/276 (78.62%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           M CQ+  LVQ + +LY KIS L+SLKPS+DVN LF QLV TC PP P+   IDV+K+   
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN---IDVTKMCDR 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASH-ENPLENLSIFPYYSNYLKLAHLEFSILER 120
           VQ++R +LIK+CG AE  LE+H+SSIL S+ +NPL +L+IFPYY+NYLKL  LEF +LE+
Sbjct: 61  VQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQ 120

Query: 121 HAQ---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLER 180
           +     P  VAF+GSG LP TSI++AS +L  T FHNFD+DPSAN+ A+ LV+ D D+ +
Sbjct: 121 NLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 181 RMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240
           RM FHT DIM+VT  L+ F VVFLAALVGM +EEKVKVIEHL K+M  GA+LMLRSA+G 
Sbjct: 181 RMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGP 240

Query: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           RAFLYP+VE  DLQGFE+L+++HPTD+VINSVV+++
Sbjct: 241 RAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of MC08g2198 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.7e-83
Identity = 157/277 (56.68%), Postives = 211/277 (76.17%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           M CQ  L+V+++ +LY +IS L+SLKPSK+V++LF QLV TC P   +   IDV+ +   
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTN---IDVTNMCEE 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILAS---HENPLENLSIFPYYSNYLKLAHLEFSIL 120
           V+ MR++LIKLCGEAE  LE H+S+IL S    +NPL++L IFPYYSNYLKL  LEF +L
Sbjct: 61  VKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL 120

Query: 121 ERHAQ--PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLE 180
            +H+   P+K+AFVGSG +P TSI++A  +LP T FHNFD+D  AN  A+ LV+ D DL 
Sbjct: 121 SQHSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLS 180

Query: 181 RRMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYG 240
           +RM+FHT D++  T  L  + VVFLAALVGM++E KVK IEHL K+M  GA+LMLRSA+ 
Sbjct: 181 KRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHA 240

Query: 241 ARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
            RAFLYP+V++ DL+GF++LT++HPTD+V+NSVV+AR
Sbjct: 241 LRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of MC08g2198 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.6e-82
Identity = 157/276 (56.88%), Postives = 211/276 (76.45%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKL-GS 60
           M C+  L+V+++ +LY++IS L+SLKPSK+V++LF QLV TC P   +   IDV+++   
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTN---IDVTEIHDE 60

Query: 61  AVQKMRSHLIKLCGEAEALLEHHYSSILASHE-NPLENLSIFPYYSNYLKLAHLEFSILE 120
            V+ MRSHLIKLCGEAE  LE H+S+IL S E NPL +L IFPYY+NYLKL  LEF +L 
Sbjct: 61  KVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLS 120

Query: 121 RHAQ--PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLER 180
           +H    P+KVAF+GSG +P TSI++A  +LP T FHNFD+D  AN  A+ LV+ D DL +
Sbjct: 121 QHTTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSK 180

Query: 181 RMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240
           RM+FHT D++     L  + VVFLAALVGM++E KVK IEHL K+M  GA++MLRSA+G 
Sbjct: 181 RMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGL 240

Query: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           RAFLYP+V++ DL+GFE+LT++HP+D+V+NSVV+AR
Sbjct: 241 RAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of MC08g2198 vs. NCBI nr
Match: XP_022156409.1 (probable nicotianamine synthase 4 [Momordica charantia])

HSP 1 Score: 530 bits (1366), Expect = 1.05e-189
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA
Sbjct: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120
           VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH
Sbjct: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120

Query: 121 AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVF 180
           AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVF
Sbjct: 121 AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVF 180

Query: 181 HTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFL 240
           HTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFL
Sbjct: 181 HTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFL 240

Query: 241 YPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           YPVVEARDLQGFEILTVFHPTDEVINSVVLARL
Sbjct: 241 YPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of MC08g2198 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 441 bits (1133), Expect = 3.86e-154
Identity = 230/277 (83.03%), Postives = 249/277 (89.89%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           MCCQ +LLVQK+SELYHKISTL+SLKPSKDVNSLF+QLVL C+PPP SP  IDVS L SA
Sbjct: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFTQLVLICSPPPSSPSSIDVSTLCSA 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120
           VQ MRSHLIKLCGEAEALLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFSIL  H
Sbjct: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120

Query: 121 AQ---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERR 180
           A    PSKVAFVGSGALPF+SIIMA KYLP TE HNFD+DPSANAKAA LVAGD +L +R
Sbjct: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPKTELHNFDIDPSANAKAAMLVAGDAELSKR 180

Query: 181 MVFHTADIMEVTG-ELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240
           MVFHT DIM+V   ELR F+VVFLAALVGMEREEKVKVIEHL K+MGGGALLMLRSAYGA
Sbjct: 181 MVFHTTDIMDVASDELREFEVVFLAALVGMEREEKVKVIEHLRKSMGGGALLMLRSAYGA 240

Query: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           RAFLYPVVEA DL+GFEILTVFHPTDEVINS+VLARL
Sbjct: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 277

[Close]

BLAST of MC08g2198 vs. NCBI nr
Match: XP_022965879.1 (nicotianamine synthase [Cucurbita maxima])

HSP 1 Score: 421 bits (1083), Expect = 1.90e-146
Identity = 223/282 (79.08%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPP---SPPPIDVS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC PPPP   SPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILERH---AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180
           IL R+   A PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA  LVA D 
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLERRMVFHTADIMEVTGE-LRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240
           DL RRM FHT DIM+VT + LR F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of MC08g2198 vs. NCBI nr
Match: XP_022938003.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 421 bits (1082), Expect = 2.70e-146
Identity = 222/282 (78.72%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPP---SPPPIDVS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC PPPP   SPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILERHAQ---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180
           IL R+     PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA ALVA D 
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLERRMVFHTADIMEVTGELRG-FQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240
           DL RRM FHT DIM+VT ++ G F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of MC08g2198 vs. NCBI nr
Match: KAG7021049.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 419 bits (1077), Expect = 1.56e-145
Identity = 220/282 (78.01%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPP---SPPPIDVS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC PPPP   SPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILERH---AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180
           IL R+   + PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA  LVA D 
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180

Query: 181 DLERRMVFHTADIMEVTGELRG-FQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240
           DL +RM+FHT DIM+VT  + G F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of MC08g2198 vs. ExPASy TrEMBL
Match: A0A6J1DTD8 (probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309 PE=3 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 5.06e-190
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA
Sbjct: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120
           VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH
Sbjct: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120

Query: 121 AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVF 180
           AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVF
Sbjct: 121 AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRMVF 180

Query: 181 HTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFL 240
           HTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFL
Sbjct: 181 HTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARAFL 240

Query: 241 YPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           YPVVEARDLQGFEILTVFHPTDEVINSVVLARL
Sbjct: 241 YPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of MC08g2198 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 421 bits (1083), Expect = 9.19e-147
Identity = 223/282 (79.08%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPP---SPPPIDVS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC PPPP   SPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILERH---AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180
           IL R+   A PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA  LVA D 
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLERRMVFHTADIMEVTGE-LRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240
           DL RRM FHT DIM+VT + LR F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of MC08g2198 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 1.31e-146
Identity = 222/282 (78.72%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPP---SPPPIDVS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC PPPP   SPP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILERHAQ---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180
           IL R+     PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA ALVA D 
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLERRMVFHTADIMEVTGELRG-FQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240
           DL RRM FHT DIM+VT ++ G F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of MC08g2198 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 418 bits (1075), Expect = 1.36e-145
Identity = 223/279 (79.93%), Postives = 244/279 (87.46%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPP---PSPPPIDVSKL 60
           MCCQ +LLVQK+SELYHKISTL+SLKPSK VNSLFSQLVL    PP    SP  IDVS L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  GSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSIL 120
            S++Q MRS+LIKLCGEAE+LLEHHYS+IL+SH+NPL++LS+FPYYSNYLKL HLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 ERHAQP--SKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLE 180
             H  P  SKVAFVGSGALPF+SIIMA KYLP TEFHNFD+DPSANAKA+ LVAGD +L 
Sbjct: 121 NHHITPPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVELS 180

Query: 181 RRMVFHTADIMEV-TGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAY 240
           RRMVFHT DIMEV + ELR F+VVFLAALVGMEREEK KVIEHL KNM GGALLMLRSAY
Sbjct: 181 RRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSAY 240

Query: 241 GARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           GARAFLYPVVEA DL+GFEILTVFHPTDEVINS+VLARL
Sbjct: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of MC08g2198 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 411 bits (1056), Expect = 1.03e-142
Identity = 219/280 (78.21%), Postives = 241/280 (86.07%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           MCCQ +LLVQK+SELYHKISTL+SLKPSK VNSLFSQLVL    P  S   IDVS L S 
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPSSSS--IDVSTLCST 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILERH 120
           +Q MRSHLIKLCGEAE+LLEHHYS+IL+SH+NPL++LS+FPYYSNYLKL HLEFSIL  H
Sbjct: 61  IQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120

Query: 121 AQ------PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDL 180
           A       PSKVAFVGSGALPF+SIIMA KY P TEFHNFD+DPSANAKA+ LVAGD +L
Sbjct: 121 APHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVEL 180

Query: 181 ERRMVFHTADIMEV-TGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSA 240
            RRMVFHT DIM+V + ELR F+VVFLAALVGMEREEK KVIEHL KNM GGALLMLRSA
Sbjct: 181 SRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRSA 240

Query: 241 YGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273
           YGARAF+YPVVEA DL+GF+ILTVFHPTDEVINS+VLARL
Sbjct: 241 YGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of MC08g2198 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 337.0 bits (863), Expect = 1.4e-92
Identity = 170/274 (62.04%), Postives = 219/274 (79.93%), Query Frame = 0


Query: 3   CQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSAVQ 62
           CQ+  LV K+ +LY KIS L++LKP +DV++LF QLV TC PP P+   IDV+K+   +Q
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN---IDVTKMSENIQ 63

Query: 63  KMRSHLIKLCGEAEALLEHHYSSILASHE-NPLENLSIFPYYSNYLKLAHLEFSILERHA 122
           +MRS+LIK+CGEAE  LEHH+SSIL S E NPL +L++FPYY+NYLKL+ LEF +LE++ 
Sbjct: 64  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 123

Query: 123 Q---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERRM 182
               P  VAF+GSG LP TS+++AS +L  + FHNFD+DPSAN  AA LV+ D DL +RM
Sbjct: 124 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 183

Query: 183 VFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGARA 242
            FHT DIM+VT  L+GF VVFLAALVGM+++EKVKV+EHL K+M  GALLMLRSA+G RA
Sbjct: 184 FFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRA 243

Query: 243 FLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           FLYP+VE  DL+GFE+L+V+HPTDEVINS+V++R
Sbjct: 244 FLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of MC08g2198 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 327.8 bits (839), Expect = 8.4e-90
Identity = 170/276 (61.59%), Postives = 217/276 (78.62%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           M CQ+  LVQ + +LY KIS L+SLKPS+DVN LF QLV TC PP P+   IDV+K+   
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN---IDVTKMCDR 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASH-ENPLENLSIFPYYSNYLKLAHLEFSILER 120
           VQ++R +LIK+CG AE  LE+H+SSIL S+ +NPL +L+IFPYY+NYLKL  LEF +LE+
Sbjct: 61  VQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQ 120

Query: 121 HAQ---PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLER 180
           +     P  VAF+GSG LP TSI++AS +L  T FHNFD+DPSAN+ A+ LV+ D D+ +
Sbjct: 121 NLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 181 RMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240
           RM FHT DIM+VT  L+ F VVFLAALVGM +EEKVKVIEHL K+M  GA+LMLRSA+G 
Sbjct: 181 RMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGP 240

Query: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           RAFLYP+VE  DLQGFE+L+++HPTD+VINSVV+++
Sbjct: 241 RAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of MC08g2198 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 308.9 bits (790), Expect = 4.0e-84
Identity = 157/277 (56.68%), Postives = 211/277 (76.17%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60
           M CQ  L+V+++ +LY +IS L+SLKPSK+V++LF QLV TC P   +   IDV+ +   
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTN---IDVTNMCEE 60

Query: 61  VQKMRSHLIKLCGEAEALLEHHYSSILAS---HENPLENLSIFPYYSNYLKLAHLEFSIL 120
           V+ MR++LIKLCGEAE  LE H+S+IL S    +NPL++L IFPYYSNYLKL  LEF +L
Sbjct: 61  VKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL 120

Query: 121 ERHAQ--PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLE 180
            +H+   P+K+AFVGSG +P TSI++A  +LP T FHNFD+D  AN  A+ LV+ D DL 
Sbjct: 121 SQHSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLS 180

Query: 181 RRMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYG 240
           +RM+FHT D++  T  L  + VVFLAALVGM++E KVK IEHL K+M  GA+LMLRSA+ 
Sbjct: 181 KRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHA 240

Query: 241 ARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
            RAFLYP+V++ DL+GF++LT++HPTD+V+NSVV+AR
Sbjct: 241 LRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of MC08g2198 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 157/276 (56.88%), Postives = 211/276 (76.45%), Query Frame = 0


Query: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKL-GS 60
           M C+  L+V+++ +LY++IS L+SLKPSK+V++LF QLV TC P   +   IDV+++   
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTN---IDVTEIHDE 60

Query: 61  AVQKMRSHLIKLCGEAEALLEHHYSSILASHE-NPLENLSIFPYYSNYLKLAHLEFSILE 120
            V+ MRSHLIKLCGEAE  LE H+S+IL S E NPL +L IFPYY+NYLKL  LEF +L 
Sbjct: 61  KVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLS 120

Query: 121 RHAQ--PSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLER 180
           +H    P+KVAF+GSG +P TSI++A  +LP T FHNFD+D  AN  A+ LV+ D DL +
Sbjct: 121 QHTTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSK 180

Query: 181 RMVFHTADIMEVTGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240
           RM+FHT D++     L  + VVFLAALVGM++E KVK IEHL K+M  GA++MLRSA+G 
Sbjct: 181 RMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGL 240

Query: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLAR 273
           RAFLYP+V++ DL+GFE+LT++HP+D+V+NSVV+AR
Sbjct: 241 RAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of MC08g2198 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-08
Identity = 30/53 (56.60%), Postives = 40/53 (75.47%), Query Frame = 0


Query: 222 MGGGALLMLRSAYGARAFLYPVVEARDLQGFEILTVFHP--TDEVINSVVLAR 273
           M  GALLMLRSA G R+FLY  V+  DL+GFE+L ++HP  +D  +NSV++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of MC08g2198 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	1.9e-91	62.04	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9XGI7	3.1e-89	60.22	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	1.2e-88	61.59	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	5.7e-83	56.68	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	1.6e-82	56.88	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of MC08g2198 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156409.1	1.05e-189	100.00	probable nicotianamine synthase 4 [Momordica charantia]	[more]
XP_038891237.1	3.86e-154	83.03	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_022965879.1	1.90e-146	79.08	nicotianamine synthase [Cucurbita maxima]	[more]
XP_022938003.1	2.70e-146	78.72	nicotianamine synthase-like [Cucurbita moschata]	[more]
KAG7021049.1	1.56e-145	78.01	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC08g2198 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DTD8	5.06e-190	100.00	probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309...	[more]
A0A6J1HS54	9.19e-147	79.08	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A6J1FHL8	1.31e-146	78.72	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]
A0A1S3BXJ6	1.36e-145	79.93	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A0A0LJ09	1.03e-142	78.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]

BLAST of MC08g2198 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	1.4e-92	62.04	nicotianamine synthase 4	[more]
AT1G09240.1	8.4e-90	61.59	nicotianamine synthase 3	[more]
AT5G04950.1	4.0e-84	56.68	nicotianamine synthase 1	[more]
AT5G56080.1	1.2e-83	56.88	nicotianamine synthase 2	[more]
AT4G26483.1	1.2e-08	56.60	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 6..272 e-value: 6.7E-112 score: 373.3
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..272
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..273 score: 53.37331
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 7..273 e-value: 8.1E-62 score: 210.7
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..272

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2198.1	MC08g2198.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity