MC08g2097 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2097
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Auxin-responsive protein
Location	MC08: 29793483 .. 29793794 (+)
RNA-Seq Expression	MC08g2097
Synteny	MC08g2097

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTGCGGCGGAGTACTTGAAGAAGAGGAGGTGGCCGGAGAGGAGAGCGGTGGCGGCGGAGGAGGATGTGTCGGCGGTGACGGTGGTGCTGGAGGGGCGGGCGATCTGCCAGCGGATCAGCCTCCACAAACATGCAAGCTACGAGAGCCTGGCCAAAGCCCTCAGAGGGATGTTCGTGCCGGAGGACGACGGCGGCGGAGGAGACTCCGGCTCCGGCGAGCGGCGGCTTGACCTTTCGAACGCGGTTCCGGGTCATCTTGTTGCTTATGAAGACATTGAAAACGATCTTCTTCTTGCTGGTGACCTCAATTGG

mRNA sequence

GCTGCGGCGGAGTACTTGAAGAAGAGGAGGTGGCCGGAGAGGAGAGCGGTGGCGGCGGAGGAGGATGTGTCGGCGGTGACGGTGGTGCTGGAGGGGCGGGCGATCTGCCAGCGGATCAGCCTCCACAAACATGCAAGCTACGAGAGCCTGGCCAAAGCCCTCAGAGGGATGTTCGTGCCGGAGGACGACGGCGGCGGAGGAGACTCCGGCTCCGGCGAGCGGCGGCTTGACCTTTCGAACGCGGTTCCGGGTCATCTTGTTGCTTATGAAGACATTGAAAACGATCTTCTTCTTGCTGGTGACCTCAATTGG

Coding sequence (CDS)

GCTGCGGCGGAGTACTTGAAGAAGAGGAGGTGGCCGGAGAGGAGAGCGGTGGCGGCGGAGGAGGATGTGTCGGCGGTGACGGTGGTGCTGGAGGGGCGGGCGATCTGCCAGCGGATCAGCCTCCACAAACATGCAAGCTACGAGAGCCTGGCCAAAGCCCTCAGAGGGATGTTCGTGCCGGAGGACGACGGCGGCGGAGGAGACTCCGGCTCCGGCGAGCGGCGGCTTGACCTTTCGAACGCGGTTCCGGGTCATCTTGTTGCTTATGAAGACATTGAAAACGATCTTCTTCTTGCTGGTGACCTCAATTGG

Protein sequence

AAAEYLKKRRWPERRAVAAEEDVSAVTVVLEGRAICQRISLHKHASYESLAKALRGMFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW

Homology

[Close]

BLAST of MC08g2097 vs. ExPASy Swiss-Prot
Match: Q9FKM7 (Auxin-responsive protein IAA33 OS=Arabidopsis thaliana OX=3702 GN=IAA33 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.2e-22
Identity = 55/82 (67.07%), Postives = 60/82 (73.17%), Query Frame = 0


Query: 23  VSAVTVVLEGRAICQRISLHKHASYESLAKALRGMFVPEDDGGGGDSGSGERRLDLSNAV 82
           V  VTVVLEGR+ICQRISL KH SY+SLA ALR MFV        D       LDLSNA+
Sbjct: 72  VPPVTVVLEGRSICQRISLDKHGSYQSLASALRQMFV--------DGADSTDDLDLSNAI 131

Query: 83  PGHLVAYEDIENDLLLAGDLNW 105
           PGHL+AYED+ENDLLLAGDL W
Sbjct: 132 PGHLIAYEDMENDLLLAGDLTW 145

[Close]

BLAST of MC08g2097 vs. ExPASy Swiss-Prot
Match: Q69VE0 (Auxin-responsive protein IAA23 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA23 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.3e-07
Identity = 36/102 (35.29%), Postives = 49/102 (48.04%), Query Frame = 0


Query: 11  WPERRAV---AAEED---VSAVTVVLEGRAICQRISLHKHASYESLAKALRGMFVP--ED 70
           WP  RA    A  ED      V V ++G    +++ L  HA Y  L +AL GMF      
Sbjct: 65  WPPVRAYRRNALREDSARAKLVKVAVDGAPYLRKVDLAAHAGYAPLLRALHGMFASCLAV 124

Query: 71  DGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 105
            GGGG  G G + +DL      ++  YED + D +L GD+ W
Sbjct: 125 RGGGGGDGEGTKLVDLVTGAE-YVPTYEDKDGDWMLVGDVPW 165

[Close]

BLAST of MC08g2097 vs. NCBI nr
Match: XP_022153356.1 (auxin-responsive protein IAA33 [Momordica charantia])

HSP 1 Score: 202 bits (514), Expect = 9.74e-65
Identity = 104/108 (96.30%), Postives = 104/108 (96.30%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRAVAAEEDVSAV----TVVLEGRAICQRISLHKHASYESLAKALRG 60
           AAAEYLKKRRWPERRAVAAEEDVSAV    TVVLEGRAICQRISLHKHASYESLAKALRG
Sbjct: 13  AAAEYLKKRRWPERRAVAAEEDVSAVIPAVTVVLEGRAICQRISLHKHASYESLAKALRG 72

Query: 61  MFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 104
           MFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW
Sbjct: 73  MFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 120

[Close]

BLAST of MC08g2097 vs. NCBI nr
Match: XP_023538318.1 (auxin-responsive protein IAA33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 154 bits (389), Expect = 1.59e-45
Identity = 86/121 (71.07%), Postives = 92/121 (76.03%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRA-------------VAAEEDVSAV----TVVLEGRAICQRISLHK 60
           AAAEY K RRWPE +                 EED +AV    TVVLEGRAICQRISLHK
Sbjct: 16  AAAEYFKMRRWPETQLGPPPLQQQQMEEEEEEEEDFAAVIPPVTVVLEGRAICQRISLHK 75

Query: 61  HASYESLAKALRGMFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLN 104
           HASYESLAKALR MFV +DDGGGG+  SGER+LDL+NAVPGHLVAYEDIENDLLLAGDLN
Sbjct: 76  HASYESLAKALRRMFVDDDDGGGGEP-SGERQLDLANAVPGHLVAYEDIENDLLLAGDLN 135

[Close]

BLAST of MC08g2097 vs. NCBI nr
Match: XP_038890972.1 (auxin-responsive protein IAA33 [Benincasa hispida])

HSP 1 Score: 154 bits (388), Expect = 1.93e-45
Identity = 85/116 (73.28%), Postives = 91/116 (78.45%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRAVA-------AEEDVSAV----TVVLEGRAICQRISLHKHASYES 60
           AAAE  K RRWPERR           EED +AV    TVVLEGRAICQRISLHKHASYES
Sbjct: 16  AAAECFKMRRWPERRVGPPQQAEGEGEEDFAAVIPPVTVVLEGRAICQRISLHKHASYES 75

Query: 61  LAKALRGMFVPEDDGGGGDS-GSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 104
           LAKALR MFV ++DGGGG    SGER+LDL+NAVPGH+VAYEDIENDLLLAGDLNW
Sbjct: 76  LAKALRRMFVDDEDGGGGGGENSGERQLDLTNAVPGHIVAYEDIENDLLLAGDLNW 131

[Close]

BLAST of MC08g2097 vs. NCBI nr
Match: XP_022969694.1 (auxin-responsive protein IAA33 [Cucurbita maxima])

HSP 1 Score: 152 bits (384), Expect = 1.00e-44
Identity = 85/124 (68.55%), Postives = 93/124 (75.00%), Query Frame = 0


Query: 1   AAAEYLKKRRWPE------------RRAVAAEEDVSAV----TVVLEGRAICQRISLHKH 60
           AAAEY K RRWPE            ++ +  EED +AV    TVVLEGRAICQRISLHKH
Sbjct: 15  AAAEYFKMRRWPETQLGPPPPLQQQQQQMEEEEDFAAVIPPVTVVLEGRAICQRISLHKH 74

Query: 61  ASYESLAKALRGMFVPEDDGGGGDSG----SGERRLDLSNAVPGHLVAYEDIENDLLLAG 104
           ASYESLAKALR MFV +DDGG G  G    SG+R+LDL+NAVPGHLVAYEDIENDLLLAG
Sbjct: 75  ASYESLAKALRRMFVDDDDGGCGSGGGGEPSGDRQLDLANAVPGHLVAYEDIENDLLLAG 134

[Close]

BLAST of MC08g2097 vs. NCBI nr
Match: KAG7020902.1 (Auxin-responsive protein IAA33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 149 bits (377), Expect = 7.33e-44
Identity = 85/126 (67.46%), Postives = 91/126 (72.22%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRA--------------VAAEEDVSAV----TVVLEGRAICQRISLH 60
           AAAEY K RRWPE +                  EED +AV    TVVLEGRAICQRISLH
Sbjct: 17  AAAEYFKMRRWPETQLGPPPLQQQQQRMEEEEEEEDFAAVIPPVTVVLEGRAICQRISLH 76

Query: 61  KHASYESLAKALRGMFVPEDDGG----GGDSGSGERRLDLSNAVPGHLVAYEDIENDLLL 104
           KHASYESLAKALR MFV +DDGG    GG   SG+R+LDL+NAVPGHLVAYEDIENDLLL
Sbjct: 77  KHASYESLAKALRRMFVDDDDGGCGGGGGGEPSGDRQLDLANAVPGHLVAYEDIENDLLL 136

[Close]

BLAST of MC08g2097 vs. ExPASy TrEMBL
Match: A0A6J1DKE3 (Auxin-responsive protein OS=Momordica charantia OX=3673 GN=LOC111020871 PE=3 SV=1)

HSP 1 Score: 202 bits (514), Expect = 4.71e-65
Identity = 104/108 (96.30%), Postives = 104/108 (96.30%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRAVAAEEDVSAV----TVVLEGRAICQRISLHKHASYESLAKALRG 60
           AAAEYLKKRRWPERRAVAAEEDVSAV    TVVLEGRAICQRISLHKHASYESLAKALRG
Sbjct: 13  AAAEYLKKRRWPERRAVAAEEDVSAVIPAVTVVLEGRAICQRISLHKHASYESLAKALRG 72

Query: 61  MFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 104
           MFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW
Sbjct: 73  MFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 120

[Close]

BLAST of MC08g2097 vs. ExPASy TrEMBL
Match: A0A6J1I3D9 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111468644 PE=3 SV=1)

HSP 1 Score: 152 bits (384), Expect = 4.85e-45
Identity = 85/124 (68.55%), Postives = 93/124 (75.00%), Query Frame = 0


Query: 1   AAAEYLKKRRWPE------------RRAVAAEEDVSAV----TVVLEGRAICQRISLHKH 60
           AAAEY K RRWPE            ++ +  EED +AV    TVVLEGRAICQRISLHKH
Sbjct: 15  AAAEYFKMRRWPETQLGPPPPLQQQQQQMEEEEDFAAVIPPVTVVLEGRAICQRISLHKH 74

Query: 61  ASYESLAKALRGMFVPEDDGGGGDSG----SGERRLDLSNAVPGHLVAYEDIENDLLLAG 104
           ASYESLAKALR MFV +DDGG G  G    SG+R+LDL+NAVPGHLVAYEDIENDLLLAG
Sbjct: 75  ASYESLAKALRRMFVDDDDGGCGSGGGGEPSGDRQLDLANAVPGHLVAYEDIENDLLLAG 134

[Close]

BLAST of MC08g2097 vs. ExPASy TrEMBL
Match: A0A5A7UPU6 (Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold222G00510 PE=3 SV=1)

HSP 1 Score: 147 bits (372), Expect = 1.04e-43
Identity = 83/111 (74.77%), Postives = 87/111 (78.38%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRA---VAAEEDVSAV----TVVLEGRAICQRISLHKHASYESLAKA 60
           AAAEY K  RW ERR      AEED SAV    TVVLEGRAICQRISLHKH SYESLAKA
Sbjct: 11  AAAEYFKMARWAERRVGPPQQAEEDFSAVIPPVTVVLEGRAICQRISLHKHGSYESLAKA 70

Query: 61  LRGMFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 104
           LR MFV +DD G     SGERRLDL+NAVPG++VAYEDIENDLLLAGDLNW
Sbjct: 71  LRRMFVDDDDEG---EESGERRLDLTNAVPGYIVAYEDIENDLLLAGDLNW 118

[Close]

BLAST of MC08g2097 vs. ExPASy TrEMBL
Match: A0A1S3BRI6 (Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103492920 PE=3 SV=1)

HSP 1 Score: 147 bits (372), Expect = 1.70e-43
Identity = 83/111 (74.77%), Postives = 87/111 (78.38%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRA---VAAEEDVSAV----TVVLEGRAICQRISLHKHASYESLAKA 60
           AAAEY K  RW ERR      AEED SAV    TVVLEGRAICQRISLHKH SYESLAKA
Sbjct: 11  AAAEYFKMARWAERRVGPPQQAEEDFSAVIPPVTVVLEGRAICQRISLHKHGSYESLAKA 70

Query: 61  LRGMFVPEDDGGGGDSGSGERRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 104
           LR MFV +DD G     SGERRLDL+NAVPG++VAYEDIENDLLLAGDLNW
Sbjct: 71  LRRMFVDDDDEG---EESGERRLDLTNAVPGYIVAYEDIENDLLLAGDLNW 118

[Close]

BLAST of MC08g2097 vs. ExPASy TrEMBL
Match: A0A6J1FIF6 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111444417 PE=3 SV=1)

HSP 1 Score: 148 bits (373), Expect = 2.49e-43
Identity = 85/129 (65.89%), Postives = 91/129 (70.54%), Query Frame = 0


Query: 1   AAAEYLKKRRWPERRAVAA-----------------EEDVSAV----TVVLEGRAICQRI 60
           AAAEY K RRWPE +  +                  EED +AV    TVVLEGRAICQRI
Sbjct: 13  AAAEYFKMRRWPETQLGSPPLQQQQQQMEEEEEEEEEEDFAAVIPPVTVVLEGRAICQRI 72

Query: 61  SLHKHASYESLAKALRGMFVPEDDGGGGDSGSGE----RRLDLSNAVPGHLVAYEDIEND 104
           SLHKHASYESLAKALR MFV +DDGG G  G GE    R+LDL+NAVPGHLVAYEDIEND
Sbjct: 73  SLHKHASYESLAKALRRMFVDDDDGGCGGGGGGEPSCDRQLDLANAVPGHLVAYEDIEND 132

[Close]

BLAST of MC08g2097 vs. TAIR 10
Match: AT5G57420.1 (indole-3-acetic acid inducible 33 )

HSP 1 Score: 107.1 bits (266), Expect = 8.8e-24
Identity = 55/82 (67.07%), Postives = 60/82 (73.17%), Query Frame = 0


Query: 23  VSAVTVVLEGRAICQRISLHKHASYESLAKALRGMFVPEDDGGGGDSGSGERRLDLSNAV 82
           V  VTVVLEGR+ICQRISL KH SY+SLA ALR MFV        D       LDLSNA+
Sbjct: 72  VPPVTVVLEGRSICQRISLDKHGSYQSLASALRQMFV--------DGADSTDDLDLSNAI 131

Query: 83  PGHLVAYEDIENDLLLAGDLNW 105
           PGHL+AYED+ENDLLLAGDL W
Sbjct: 132 PGHLIAYEDMENDLLLAGDLTW 145

[Close]

BLAST of MC08g2097 vs. TAIR 10
Match: AT4G29080.1 (phytochrome-associated protein 2 )

HSP 1 Score: 43.1 bits (100), Expect = 1.6e-04
Identity = 30/91 (32.97%), Postives = 42/91 (46.15%), Query Frame = 0


Query: 20  EEDVSAVTVVLEGRAICQRISLHKHASYESLAKALRGMFVP------EDDGGGGDSGSGE 79
           E+    V V +EG    ++I L  + SY  L+ AL  MF           GG G  G  E
Sbjct: 182 EQPCLYVKVSMEGAPYLRKIDLKTYKSYLELSSALEKMFSCFTIGQFGSHGGCGRDGLNE 241

Query: 80  RRLDLSNAVPGHLVAYEDIENDLLLAGDLNW 105
            RL        ++V YED ++D +L GD+ W
Sbjct: 242 SRLTDLLRGSEYVVTYEDKDSDWMLVGDVPW 272

The following BLAST results are available for this feature:

BLAST of MC08g2097 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKM7	1.2e-22	67.07	Auxin-responsive protein IAA33 OS=Arabidopsis thaliana OX=3702 GN=IAA33 PE=1 SV=...	[more]
Q69VE0	4.3e-07	35.29	Auxin-responsive protein IAA23 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA23...	[more]

BLAST of MC08g2097 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153356.1	9.74e-65	96.30	auxin-responsive protein IAA33 [Momordica charantia]	[more]
XP_023538318.1	1.59e-45	71.07	auxin-responsive protein IAA33 [Cucurbita pepo subsp. pepo]	[more]
XP_038890972.1	1.93e-45	73.28	auxin-responsive protein IAA33 [Benincasa hispida]	[more]
XP_022969694.1	1.00e-44	68.55	auxin-responsive protein IAA33 [Cucurbita maxima]	[more]
KAG7020902.1	7.33e-44	67.46	Auxin-responsive protein IAA33, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of MC08g2097 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKE3	4.71e-65	96.30	Auxin-responsive protein OS=Momordica charantia OX=3673 GN=LOC111020871 PE=3 SV=...	[more]
A0A6J1I3D9	4.85e-45	68.55	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111468644 PE=3 SV=1	[more]
A0A5A7UPU6	1.04e-43	74.77	Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A1S3BRI6	1.70e-43	74.77	Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103492920 PE=3 SV=1	[more]
A0A6J1FIF6	2.49e-43	65.89	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111444417 PE=3 SV=1	[more]

BLAST of MC08g2097 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57420.1	8.8e-24	67.07	indole-3-acetic acid inducible 33	[more]
AT4G29080.1	1.6e-04	32.97	phytochrome-associated protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 19..104 e-value: 5.1E-9 score: 36.4
None	No IPR available	GENE3D	3.10.20.90		coord: 18..104 e-value: 1.9E-13 score: 52.2
None	No IPR available	PANTHER	PTHR31734:SF7	AUXIN-RESPONSIVE PROTEIN IAA33	coord: 15..104
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 15..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g2097.1	MC08g2097.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus