MC08g2074 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2074 vs. ExPASy Swiss-Prot
Match: Q80XQ2 (TBC1 domain family member 5 OS=Mus musculus OX=10090 GN=Tbc1d5 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 1.5e-33
Identity = 114/420 (27.14%), Postives = 182/420 (43.33%), Query Frame = 0

Query: 39  RGVRWRINLGVLPSSSLASIDDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAID 98
           R + W++ L VLP      I  ++ +    R  Y+ ++   + +P   +  +   D+ I+
Sbjct: 87  RSICWKLFLCVLPQDKSQWISKIKEL----RAWYSSIKEIHITNP---RKAAGQQDLMIN 146

Query: 99  NPLSQNPDSMWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHP 158
           NPLSQ+  S+W +FF+  EL  M++QD+ R +PE   +FQ    + +L  +L  +  ++ 
Sbjct: 147 NPLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEM-QFFQQENVRKILTDVLFCYARENE 206

Query: 159 QFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNR 218
           Q  Y+QGMHELLAP+++ LH D                                      
Sbjct: 207 QLLYKQGMHELLAPIIFTLHCD-------------------------------------- 266

Query: 219 LDATEDEVGVHGNEENVKSLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMF 278
                 +  +H +E    S                      E+  +L+  ++EHDAYAMF
Sbjct: 267 -----HQAFLHASESAQPS---------------------EEMKTLLNPEYLEHDAYAMF 326

Query: 279 DALMSGAH---------GSVSMGDFFSPTPAGSSLSGLPP---VIEASAALYHLLSHVDL 338
             LM  A          G        +P P        P    V + +    HLL   D+
Sbjct: 327 SQLMETAEPWFSTFEHDGQKGKETLMAPIPFARPQDLGPTVAIVTKVNQIQDHLLKKHDI 386

Query: 339 SLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDLLTIWDEIFASDNGKFYRSDEPETSSS 398
            L+ HL  L + PQ + LRW+R+LFGREF L+DLL +WD +FA                 
Sbjct: 387 ELYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLVVWDALFA----------------- 412

Query: 399 SGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKNVDLKKLIEKAKSLQ 447
                S   + +  +  +MLLY+R +L+++ N   CL  L+++P   D+  LI KA  L+
Sbjct: 447 ----DSLNLSLVDYVFTAMLLYIRDALISS-NYQTCLGLLMHYPIIGDIHSLILKALFLR 412

BLAST of MC08g2074 vs. ExPASy Swiss-Prot
Match: Q92609 (TBC1 domain family member 5 OS=Homo sapiens OX=9606 GN=TBC1D5 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 5.6e-33
Identity = 119/423 (28.13%), Postives = 186/423 (43.97%), Query Frame = 0

Query: 39  RGVRWRINLGVLP---SSSLASIDDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDI 98
           R + W++ L VLP   S  ++ I++LR   ++ +  +    R+++             D+
Sbjct: 87  RSICWKLFLCVLPQDKSQWISRIEELRAWYSNIKEIHITNPRKVV----------GQQDL 146

Query: 99  AIDNPLSQNPDSMWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCL 158
            I+NPLSQ+  S+W +FF+  EL  M++QD+ R +PE   +FQ    + +L  +L  +  
Sbjct: 147 MINNPLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEM-QFFQQENVRKILTDVLFCYAR 206

Query: 159 QHPQFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDL 218
           ++ Q  Y+QGMHELLAP+++VLH D                                   
Sbjct: 207 ENEQLLYKQGMHELLAPIVFVLHCD----------------------------------- 266

Query: 219 KNRLDATEDEVGVHGNEENVKSLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAY 278
                    +  +H +E    S                      E+  VL+  ++EHDAY
Sbjct: 267 --------HQAFLHASESAQPS---------------------EEMKTVLNPEYLEHDAY 326

Query: 279 AMFDALMSGAH---------GSVSMGDFFSPTPAGSSLSGLPP---VIEASAALYHLLSH 338
           A+F  LM  A          G        +P P        P    V + +    HLL  
Sbjct: 327 AVFSQLMETAEPWFSTFEHDGQKGKETLMTPIPFARPQDLGPTIAIVTKVNQIQDHLLKK 386

Query: 339 VDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDLLTIWDEIFASDNGKFYRSDEPET 398
            D+ L+ HL  L + PQ + LRW+R+LFGREF L+DLL +WD +FA              
Sbjct: 387 HDIELYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLVVWDALFAD------------- 412

Query: 399 SSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKNVDLKKLIEKAK 447
                    S G  +  I V+MLLY+R +L+++ N   CL  L+++P   D+  LI KA 
Sbjct: 447 -------GLSLG-LVDYIFVAMLLYIRDALISS-NYQTCLGLLMHYPFIGDVHSLILKAL 412

BLAST of MC08g2074 vs. ExPASy Swiss-Prot
Match: Q54VM3 (TBC1 domain family member 5 homolog A OS=Dictyostelium discoideum OX=44689 GN=tbc1d5A PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 2.0e-30
Identity = 121/474 (25.53%), Postives = 211/474 (44.51%), Query Frame = 0

Query: 38  LRGVRWRINLGVLPSSSLASIDDLRRVTADSRRRYAILRRRLLVDPHISKD--------- 97
           +R + WRI LG L       ++     T  SR++Y   ++  +++P  SKD         
Sbjct: 379 VRSIFWRIALGTLSKDPTTWVER----TNSSRKKYETFKKNYIINPRNSKDQDADLQQQQ 438

Query: 98  -----GSNSPDIAIDNPLSQNPDSMWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQ 157
                    P   ID+PLSQ+ DS+W +FF +   ++ +  D+SR YP  G +F+    Q
Sbjct: 439 QQQQQQQRKPVSLIDDPLSQSEDSLWNQFFDNENAQREISHDISRTYPGLG-FFERLDIQ 498

Query: 158 GLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFA-DNFDGL 217
            ++ RIL ++  Q+P+  Y QGM+E+LAP+LY ++ D    +       D F+  N+D  
Sbjct: 499 DIMIRILFIFSKQYPKIKYLQGMNEILAPILYSVYNDSHWFNN-----RDVFSKKNYDKK 558

Query: 218 SFQDNSFKSNFDLKNRLDATED---EVGVHGNEENVKSLSELDPEIQTIILLTDAYGAEG 277
           + Q   F   FD + + D   D   +   + N  N    S     +            +G
Sbjct: 559 NKQYEHFDFVFDQQYQQDYYPDGPIQYPTNSNNFNGAGSSGSGGSVSR---------KDG 618

Query: 278 ELGIVLSE-RFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPAGSSLSGLPP--------- 337
            +G  L + ++ EHD+Y +F++LM+       +G +F+  P+    S  PP         
Sbjct: 619 GIGAFLRDPQYFEHDSYFIFESLMT------IVGKWFTSPPS----SPQPPPRVQGQFKK 678

Query: 338 -----------------VIEASAALYHLLSHVDLSLHAHL-VELGVEPQYFSLRWLRVLF 397
                            V++    ++  L  ++  L+++L  +LG+EP  +SLRW+R++ 
Sbjct: 679 LYDLSERDASDQAVNIVVVDQCLRMFEDLKFIEPQLYSYLKQDLGIEPHLYSLRWIRIIL 738

Query: 398 GREFSLEDLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRS 457
            + F L+ LL +WD IF                        S   F+  I ++ML+ ++ 
Sbjct: 739 AQVFPLDSLLILWDSIF----------------------KESVTEFLPYICLTMLIMIKD 798

Query: 458 SLLATENATLCLQRLLNFPKNVDLKKLIEKAKS----LQTLALQSNISSSPSLS 462
            ++  + +  CLQ L ++P   D+  L+  A S    +Q    Q NI  S  +S
Sbjct: 799 QIIEKDYSE-CLQVLFHYPVTQDMPMLLNTAYSVREKIQMAKQQYNIPISTPVS 800

BLAST of MC08g2074 vs. ExPASy Swiss-Prot
Match: Q54TA5 (TBC1 domain family member 5 homolog B OS=Dictyostelium discoideum OX=44689 GN=tbc1d5B PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.3e-26
Identity = 115/444 (25.90%), Postives = 186/444 (41.89%), Query Frame = 0

Query: 34  RFVDLRGVRWRINLGVLPSSSLASIDDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSP 93
           ++  LRG+ W++ LG L    +  +D   R     R+RY  L+     DP      SNS 
Sbjct: 281 KYSPLRGIAWKLFLGGL---DINRVDKWERDITQQRKRYEKLKEEHCFDPR----NSNS- 340

Query: 94  DIAIDNPLSQNPDSMWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLW 153
                +PLSQN DS W +FF++ + +K++  DL R +P++  +F  P  + ++  IL ++
Sbjct: 341 ---TYDPLSQNDDSPWNKFFKNLDTQKIIKIDLERTHPDN-DFFSNPVIREMMATILFVY 400

Query: 154 CLQHPQFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNF 213
              +    YRQGMHELLAP++Y+ + +     ++ +       D    + + ++   + F
Sbjct: 401 SKTNGIISYRQGMHELLAPIIYLYNQEYSSYKKLDENSSSTLVDFIYNIKYLEHDTFAIF 460

Query: 214 D--LKNRLD----ATEDEVGVHGNEENVK------SLSELDPEIQTIILLTDAYGAEGEL 273
           +  +K   D    A   +     N  N        S S       +      A       
Sbjct: 461 ERLMKFTSDWYAPAPTQQTNNSNNSNNTNNNNTTTSPSSSSSSSSSSTTTAAATTVSSST 520

Query: 274 GIVLSERFIEHDAYAMFDALMSGAHGSVS------MGDFFSPTPAGSSLSGLPP------ 333
               S   I   + +        +  S S      +G   SPT A SS S +        
Sbjct: 521 STSSSSSTITSSSSSTVSPPPPSSSSSPSPPPPPPLGS-NSPTVASSSSSSVQDNEEEEL 580

Query: 334 -------VIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDLLT 393
                  V++       LL   D  L+ HL  L +EPQ + LRW+R+LFGREF  +D+L 
Sbjct: 581 SSKCNDVVLKCKYIHSILLKQKDFELYQHLDSLDIEPQIYLLRWIRLLFGREFHFDDVLN 640

Query: 394 IWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLC 447
           IWD +FA                            I    +SML Y+R  LL ++ +   
Sbjct: 641 IWDALFAYGENLI---------------------LIDYFCISMLTYIREHLLKSD-SIYA 689

BLAST of MC08g2074 vs. ExPASy Swiss-Prot
Match: Q9NVG8 (TBC1 domain family member 13 OS=Homo sapiens OX=9606 GN=TBC1D13 PE=1 SV=3)

HSP 1 Score: 79.0 bits (193), Expect = 2.9e-13
Identity = 113/452 (25.00%), Postives = 188/452 (41.59%), Query Frame = 0

Query: 10  LSEPASATS-----SSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDDLRRV 69
           L EP+ A       S SG  C G          LR + W+I L  LP    +       +
Sbjct: 16  LKEPSIALEKLRELSFSGIPCEGG---------LRCLCWKILLNYLPLERAS----WTSI 75

Query: 70  TADSRRRYAILRRRLLVDPHISKD--GSNSPDIAI-DNPLSQNPDSMWGRFFRSAELEKM 129
            A  R  YA   R +++ P I+K   G +  D+   D+PL+ NPDS W  +F+  E+   
Sbjct: 76  LAKQRELYAQFLREMIIQPGIAKANMGVSREDVTFEDHPLNPNPDSRWNTYFKDNEVLLQ 135

Query: 130 VDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVDV 189
           +D+D+ RL P+  S+FQ       L        +  PQ  +              L   V
Sbjct: 136 IDKDVRRLCPDI-SFFQRATDYPCL-------LILDPQNEFE------------TLRKRV 195

Query: 190 ERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLK-NRLDATEDEVGVHGN--EENVKSL 249
           E+ +    L     A N  G++   +  K++     N  +   +    H    E  +   
Sbjct: 196 EQTT----LKSQTVARNRSGVTNMSSPHKNSVPSSLNEYEVLPNGCEAHWEVVERILFIY 255

Query: 250 SELDPEIQTIILLTDAYG-AEGELGIVLSERFIEH---DAYAMFDALMSGAHGSVSMGDF 309
           ++L+P I  +  + +  G          +  + EH   D +  F  LM+       + D 
Sbjct: 256 AKLNPGIAYVQGMNEIVGPLYYTFATDPNSEWKEHAEADTFFCFTNLMA------EIRDN 315

Query: 310 FSPTPAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGRE 369
           F  +    S  G+   +E    +Y  L   D+ L+  L E  ++PQ+F+ RWL +L  +E
Sbjct: 316 FIKS-LDDSQCGITYKME---KVYSTLKDKDVELYLKLQEQNIKPQFFAFRWLTLLLSQE 375

Query: 370 FSLEDLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLL 429
           F L D++ IWD +FA DN                     R  F+  +  +ML+ +R  LL
Sbjct: 376 FLLPDVIRIWDSLFADDN---------------------RFDFLLLVCCAMLMLIREQLL 397

Query: 430 ATENATLCLQRLLNFPKNVDLKKLIEKAKSLQ 447
             +  T+ ++ L ++P   D+ ++++KAK LQ
Sbjct: 436 EGD-FTVNMRLLQDYP-ITDVCQILQKAKELQ 397

BLAST of MC08g2074 vs. NCBI nr
Match: XP_022149856.1 (TBC domain-containing protein C1952.17c [Momordica charantia] >XP_022149857.1 TBC domain-containing protein C1952.17c [Momordica charantia])

HSP 1 Score: 1603 bits (4150), Expect = 0.0
Identity = 830/832 (99.76%), Postives = 830/832 (99.76%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD 60
           MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD
Sbjct: 1   MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD 60

Query: 61  LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK 120
           LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK
Sbjct: 61  LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK 120

Query: 121 MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD 180
           MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD
Sbjct: 121 MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD 180

Query: 181 VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE 240
           VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE
Sbjct: 181 VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE 240

Query: 241 LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA 300
           LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA
Sbjct: 241 LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA 300

Query: 301 GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL 360
           GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL
Sbjct: 301 GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL 360

Query: 361 LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 420
           LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT
Sbjct: 361 LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 420

Query: 421 LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS 480
           LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS
Sbjct: 421 LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS 480

Query: 481 GSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDP 540
           GSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDP
Sbjct: 481 GSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDP 540

Query: 541 SPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDS 600
           SPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDS
Sbjct: 541 SPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDS 600

Query: 601 CEKCLENAVDKRCESGIAGSENSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDEN 660
           CEKCLENAVDKRCESGIAGSENSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDEN
Sbjct: 601 CEKCLENAVDKRCESGIAGSENSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDEN 660

Query: 661 DDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGRNVV 720
           DDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGRNV 
Sbjct: 661 DDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGRNVA 720

Query: 721 SEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHSMLE 780
           SEGKGGTETSKSTGIESNPTKNIASPKIDGP SISVSSKGDGVDQNVMGTLKNLGHSMLE
Sbjct: 721 SEGKGGTETSKSTGIESNPTKNIASPKIDGPXSISVSSKGDGVDQNVMGTLKNLGHSMLE 780

Query: 781 HVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           HVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 HVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832

BLAST of MC08g2074 vs. NCBI nr
Match: XP_011648956.1 (TBC1 domain family member 5 homolog A [Cucumis sativus] >XP_031736806.1 TBC1 domain family member 5 homolog A [Cucumis sativus] >KGN61173.1 hypothetical protein Csa_021196 [Cucumis sativus])

HSP 1 Score: 1405 bits (3636), Expect = 0.0
Identity = 737/835 (88.26%), Postives = 763/835 (91.38%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKREFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKD S+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDESSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE GV GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGVDGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+S T
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSST 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+VAR NG 
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVARGNGR 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGS+  NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSRGNNAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP P KL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+EVV+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPGKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEVVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRCESGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCESGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSSRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC NDSEGNA VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDSEGNAAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N VSEGKG TE SK  G E+NP KNIA PKIDG  S SVS KGDGVDQN+MGTLKN+G S
Sbjct: 721 NAVSEGKGDTEASKLAGAENNPIKNIAPPKIDGACSTSVSGKGDGVDQNMMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. NCBI nr
Match: XP_038889834.1 (TBC1 domain family member 5-like [Benincasa hispida])

HSP 1 Score: 1403 bits (3632), Expect = 0.0
Identity = 739/835 (88.50%), Postives = 764/835 (91.50%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEPAS TSSSS  GSV H NSEDKRRFVDLRGVRWRINLGVLPS  LASI
Sbjct: 1   MALSEIVPALSEPASTTSSSSCSGSVSHRNSEDKRRFVDLRGVRWRINLGVLPS--LASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYA+LRRR LVDPH+SKDGS+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAMLRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD TEDE G  GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDPTEDEFGADGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAY M DALMSGAHG V+M DF+SPT
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYTMLDALMSGAHGEVAMADFYSPT 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS  FL SSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNRKFDRSDEPETSSSFDFLGSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISS+P LSGAYH+HSKSMVAR NG 
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSTPLLSGAYHNHSKSMVARGNGH 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGS++QNAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSRSQNAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DPSPAKL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+E VDNKDDLSVEGEVDGQ
Sbjct: 541 DPSPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDE-VDNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK +ENA DKRCESGIAGSE NSSIFSDPTSSFSG NDNE+DLN S RSSVASNLSL
Sbjct: 601 DGCEKYMENAEDKRCESGIAGSEENSSIFSDPTSSFSGANDNEHDLNDSSRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ S+VE  P   P+Q ENIPEKSGC ND EGNA VGTKDRKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSVVEGSPLPAPDQYENIPEKSGCTNDFEGNAAVGTKDRKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N  SEGK  TETSKSTG ESNP KNI  PKIDG  S SVS KGDGVDQNVMGTLKNLG S
Sbjct: 721 NAASEGKADTETSKSTGAESNPIKNIDPPKIDGACSTSVSGKGDGVDQNVMGTLKNLGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832

BLAST of MC08g2074 vs. NCBI nr
Match: XP_008451244.1 (PREDICTED: uncharacterized protein LOC103492589 [Cucumis melo] >KAA0053174.1 RabGAP-TBC domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 732/835 (87.66%), Postives = 763/835 (91.38%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR+FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKRQFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKDGS+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE G  GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGADGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+SPT
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSPT 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNTKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+V R NGL
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVGRGNGL 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGSK++NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSKSKNAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP PAKL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+E+V+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEIVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRC SGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCGSGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSNRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC ND EGN  VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDCEGNPAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N  SEGKG TE SK  G E+NP K+IA P+IDG  S S+S KGDGVDQNVMGTLKN+G S
Sbjct: 721 NAASEGKGDTEASKLAGAENNPIKHIAPPRIDGACSTSISGKGDGVDQNVMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKN LVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNHLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. NCBI nr
Match: TYK08892.1 (TBC1 domain family member 5-like protein A [Cucumis melo var. makuwa])

HSP 1 Score: 1397 bits (3615), Expect = 0.0
Identity = 731/835 (87.54%), Postives = 762/835 (91.26%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR+FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKRQFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKDGS+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE G  GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGADGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+SPT
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSPT 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNTKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+V R NGL
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVGRGNGL 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGSK++ AAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSKSKTAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP PAKL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+E+V+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEIVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRC SGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCGSGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSSRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC ND EGN  VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDCEGNPAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N  SEGKG TE SK  G E+NP K+IA P+IDG  S S+S KGDGVDQNVMGTLKN+G S
Sbjct: 721 NAASEGKGDTEASKLAGAENNPIKHIAPPRIDGACSTSISGKGDGVDQNVMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKN LVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNHLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. ExPASy TrEMBL
Match: A0A6J1D954 (TBC domain-containing protein C1952.17c OS=Momordica charantia OX=3673 GN=LOC111018184 PE=4 SV=1)

HSP 1 Score: 1603 bits (4150), Expect = 0.0
Identity = 830/832 (99.76%), Postives = 830/832 (99.76%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD 60
           MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD
Sbjct: 1   MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD 60

Query: 61  LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK 120
           LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK
Sbjct: 61  LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK 120

Query: 121 MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD 180
           MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD
Sbjct: 121 MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD 180

Query: 181 VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE 240
           VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE
Sbjct: 181 VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE 240

Query: 241 LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA 300
           LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA
Sbjct: 241 LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA 300

Query: 301 GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL 360
           GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL
Sbjct: 301 GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL 360

Query: 361 LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 420
           LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT
Sbjct: 361 LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 420

Query: 421 LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS 480
           LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS
Sbjct: 421 LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS 480

Query: 481 GSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDP 540
           GSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDP
Sbjct: 481 GSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDP 540

Query: 541 SPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDS 600
           SPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDS
Sbjct: 541 SPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDS 600

Query: 601 CEKCLENAVDKRCESGIAGSENSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDEN 660
           CEKCLENAVDKRCESGIAGSENSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDEN
Sbjct: 601 CEKCLENAVDKRCESGIAGSENSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDEN 660

Query: 661 DDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGRNVV 720
           DDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGRNV 
Sbjct: 661 DDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGRNVA 720

Query: 721 SEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHSMLE 780
           SEGKGGTETSKSTGIESNPTKNIASPKIDGP SISVSSKGDGVDQNVMGTLKNLGHSMLE
Sbjct: 721 SEGKGGTETSKSTGIESNPTKNIASPKIDGPXSISVSSKGDGVDQNVMGTLKNLGHSMLE 780

Query: 781 HVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           HVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 HVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832

BLAST of MC08g2074 vs. ExPASy TrEMBL
Match: A0A0A0LHC4 (Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G061030 PE=4 SV=1)

HSP 1 Score: 1405 bits (3636), Expect = 0.0
Identity = 737/835 (88.26%), Postives = 763/835 (91.38%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKREFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKD S+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDESSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE GV GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGVDGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+S T
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSST 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNSKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+VAR NG 
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVARGNGR 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGS+  NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSRGNNAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP P KL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+EVV+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPGKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEVVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRCESGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCESGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSSRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC NDSEGNA VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDSEGNAAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N VSEGKG TE SK  G E+NP KNIA PKIDG  S SVS KGDGVDQN+MGTLKN+G S
Sbjct: 721 NAVSEGKGDTEASKLAGAENNPIKNIAPPKIDGACSTSVSGKGDGVDQNMMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. ExPASy TrEMBL
Match: A0A5A7UD37 (RabGAP-TBC domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold751G00030 PE=4 SV=1)

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 732/835 (87.66%), Postives = 763/835 (91.38%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR+FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKRQFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKDGS+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE G  GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGADGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+SPT
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSPT 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNTKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+V R NGL
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVGRGNGL 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGSK++NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSKSKNAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP PAKL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+E+V+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEIVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRC SGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCGSGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSNRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC ND EGN  VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDCEGNPAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N  SEGKG TE SK  G E+NP K+IA P+IDG  S S+S KGDGVDQNVMGTLKN+G S
Sbjct: 721 NAASEGKGDTEASKLAGAENNPIKHIAPPRIDGACSTSISGKGDGVDQNVMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKN LVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNHLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. ExPASy TrEMBL
Match: A0A1S3BR33 (uncharacterized protein LOC103492589 OS=Cucumis melo OX=3656 GN=LOC103492589 PE=4 SV=1)

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 732/835 (87.66%), Postives = 763/835 (91.38%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR+FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKRQFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKDGS+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE G  GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGADGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+SPT
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSPT 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNTKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+V R NGL
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVGRGNGL 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGSK++NAAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSKSKNAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP PAKL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+E+V+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEIVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRC SGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCGSGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSNRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC ND EGN  VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDCEGNPAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N  SEGKG TE SK  G E+NP K+IA P+IDG  S S+S KGDGVDQNVMGTLKN+G S
Sbjct: 721 NAASEGKGDTEASKLAGAENNPIKHIAPPRIDGACSTSISGKGDGVDQNVMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKN LVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNHLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. ExPASy TrEMBL
Match: A0A5D3CC73 (TBC1 domain family member 5-like protein A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1411G00160 PE=4 SV=1)

HSP 1 Score: 1397 bits (3615), Expect = 0.0
Identity = 731/835 (87.54%), Postives = 762/835 (91.26%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSS--GSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASI 60
           MA SEIV ALSEP S TSSSS  GSV H  SEDKR+FVDLRGVRWRINLGVLPSSSLASI
Sbjct: 1   MAPSEIVPALSEPTSTTSSSSCSGSVFHRISEDKRQFVDLRGVRWRINLGVLPSSSLASI 60

Query: 61  DDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAEL 120
           DDLRRVTADSRRRYAILRRR LVDPH+SKDGS+SPDIA+DNPLSQNPDSMWGRFFRSAEL
Sbjct: 61  DDLRRVTADSRRRYAILRRRHLVDPHVSKDGSSSPDIAMDNPLSQNPDSMWGRFFRSAEL 120

Query: 121 EKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180
           EKMVDQDLSRLYPEHGSYFQTPGCQ LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH
Sbjct: 121 EKMVDQDLSRLYPEHGSYFQTPGCQSLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLH 180

Query: 181 VDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSL 240
           VDVERLSQVRKLYEDQFAD FDGLSFQD SFK NFD KNRLD+TEDE G  GN E+VKSL
Sbjct: 181 VDVERLSQVRKLYEDQFADKFDGLSFQDGSFKYNFDFKNRLDSTEDEFGADGNVESVKSL 240

Query: 241 SELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPT 300
           SELDPEIQTIILLTDAYGAEGELGIVLS+RFIEHDAY MFDALMSGAHG V+M DF+SPT
Sbjct: 241 SELDPEIQTIILLTDAYGAEGELGIVLSDRFIEHDAYTMFDALMSGAHGEVAMADFYSPT 300

Query: 301 PAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360
           PAG SLSGLPPVIEAS+ALYHLLSHVD SLHAHLVELGVEPQYFSLRWLRVLFGREFSLE
Sbjct: 301 PAGGSLSGLPPVIEASSALYHLLSHVDSSLHAHLVELGVEPQYFSLRWLRVLFGREFSLE 360

Query: 361 DLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420
           DLLTIWDEIFASDN KF RSDEPETSSS GFLSSSRGAFIAAIAVSMLLYLRSSLLATEN
Sbjct: 361 DLLTIWDEIFASDNTKFDRSDEPETSSSFGFLSSSRGAFIAAIAVSMLLYLRSSLLATEN 420

Query: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGL 480
           ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLA+ SNISSSP LSGAYHHHSKS+V R NGL
Sbjct: 421 ATLCLQRLLNFPKNVDLKKLIEKAKSLQTLAMHSNISSSPLLSGAYHHHSKSIVGRGNGL 480

Query: 481 SSGSVSPKTPLSLVPESYWEEKWRVLHREEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
           SSGSVSPKTPL+ VPESYWEEKWRVLH+E+EFKQSGSK++ AAQKKGWSEKVRFLYRTES
Sbjct: 481 SSGSVSPKTPLNHVPESYWEEKWRVLHKEQEFKQSGSKSKTAAQKKGWSEKVRFLYRTES 540

Query: 541 DPSPAKLAGGKKNTKSSVRRRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQ 600
           DP PAKL GGKKNTKSSVRRRLLADLSRELGA ED EKCGN+E+V+NKDDLSVEGEVDGQ
Sbjct: 541 DPFPAKLVGGKKNTKSSVRRRLLADLSRELGAEEDSEKCGNDEIVNNKDDLSVEGEVDGQ 600

Query: 601 DSCEKCLENAVDKRCESGIAGSE-NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSL 660
           D CEK LENA DKRC SGIAGSE NSSIFSDPTSSFSG NDNE DLN S RSSVASNLSL
Sbjct: 601 DGCEKYLENAEDKRCGSGIAGSEENSSIFSDPTSSFSGANDNEPDLNDSSRSSVASNLSL 660

Query: 661 DENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRKLLGKFQWFWKFGR 720
           DENDD++ SIVE     VP+QLENIPEKSGC ND EGN  VG K+RKLLGKF WFWKFGR
Sbjct: 661 DENDDQSQSIVEGSSLPVPDQLENIPEKSGCTNDCEGNPAVGAKERKLLGKFPWFWKFGR 720

Query: 721 NVVSEGKGGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHS 780
           N  SEGKG TE SK  G E+NP K+IA P+IDG  S S+S KGDGVDQNVMGTLKN+G S
Sbjct: 721 NAASEGKGDTEASKLAGAENNPIKHIAPPRIDGACSTSISGKGDGVDQNVMGTLKNIGQS 780

Query: 781 MLEHVQVIETVFQQERGQVGSLENLSKNPLVGKGQVTAMAALKELRKISNLLSEM 832
           ML+H+QVIETVFQQERGQVGSLENLSKN LVGKGQVTAMAALKELRKISNLLSEM
Sbjct: 781 MLDHIQVIETVFQQERGQVGSLENLSKNHLVGKGQVTAMAALKELRKISNLLSEM 835

BLAST of MC08g2074 vs. TAIR 10
Match: AT4G29950.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 805.4 bits (2079), Expect = 4.2e-233
Identity = 476/841 (56.60%), Postives = 585/841 (69.56%), Query Frame = 0

Query: 30  EDKRRFVDLRGVRWRINLGVLPSSSLASIDDLRRVTADSRRRYAILRRRLLVDPHISKDG 89
           E  RRF +LRG+RWR+NLGVLP  S +SIDDLR+ TA+SRRRYA LRRRLL+DPH+SKD 
Sbjct: 12  ESDRRFANLRGLRWRVNLGVLPFQS-SSIDDLRKATAESRRRYAALRRRLLIDPHLSKDV 71

Query: 90  SNSPDIAIDNPLSQNPDSMWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRI 149
            NSPD++IDNPLSQNPDS WGRFFR+AELEK +DQDLSRLYPEH SYFQ PGCQG+LRRI
Sbjct: 72  RNSPDLSIDNPLSQNPDSTWGRFFRNAELEKTLDQDLSRLYPEHWSYFQAPGCQGMLRRI 131

Query: 150 LLLWCLQHPQFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSFQDNSF 209
           LLLWCL+HP++GYRQGMHELLAPLLYVLHVDV+RLS+VRK YED F D FDGLSF++   
Sbjct: 132 LLLWCLKHPEYGYRQGMHELLAPLLYVLHVDVDRLSEVRKSYEDHFIDRFDGLSFEERDI 191

Query: 210 KSNFDLKNRL-DATEDEV-GVHGNEENVKSLSELDPEIQTIILLTDAYGAEGELGIVLSE 269
             NF+ K  L D T+DE+ G+ GN + +KSL ELDPEIQ+I+ L+DAYGAEGELGIVLSE
Sbjct: 192 TYNFEFKKFLEDFTDDEIGGIQGNSKKIKSLDELDPEIQSIVRLSDAYGAEGELGIVLSE 251

Query: 270 RFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPAGSSLSGLPPVIEASAALYHLLSHVDLS 329
           +F+EHDAY MFDALM+G HG V+M  FF+ +PA  S +GLPPV+EAS A YHLLS VD S
Sbjct: 252 KFMEHDAYCMFDALMNGVHGCVAMAGFFAYSPASGSHTGLPPVLEASTAFYHLLSFVDSS 311

Query: 330 LHAHLVELGVEPQYFSLRWLRVLFGREFSLEDLLTIWDEIFASDNGKFYRSDEPETSSSS 389
           LH+HLVELGVEPQYF LRWLRVLFGREF L+DLL +WDEIF++DN     +D   T+ S 
Sbjct: 312 LHSHLVELGVEPQYFGLRWLRVLFGREFLLQDLLIVWDEIFSADNTTRTDADN-NTNQSY 371

Query: 390 GFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKNVDLKKLIEKAKSLQT 449
               S RGA I+ +AVSM+L LRSSLLATENA  CLQRLLNFP+ +D++K+IEKAKSLQT
Sbjct: 372 KIFDSPRGALISGMAVSMILCLRSSLLATENAASCLQRLLNFPEKIDVRKIIEKAKSLQT 431

Query: 450 LALQSNI-SSSPSLSGAYHHH-SKSMVARSNGLSSGSVSPKTPLSLVPESYWEEKWRVLH 509
           LAL  ++ SS+ S++  +    S ++ AR+N   SGS SPK+PL + P+SYWE++WRVLH
Sbjct: 432 LALDDDVRSSALSINDGFDQSISPAVPARTNSFPSGSTSPKSPLIITPQSYWEDQWRVLH 491

Query: 510 R-EEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDPS-PAKLAGGKKNTK-SSVRRRLLA 569
           +  EE K+S S  Q   +KK W  +V+ L+R ES+P+  AK   GK   K SSV R LL 
Sbjct: 492 KAAEEEKKSPSPIQ---KKKPWF-RVKRLFRAESEPTHSAKSPNGKSEVKISSVARNLLE 551

Query: 570 DLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDSCEKCLENAVDKRCESGIAGSEN 629
           D +R+L  +E +E   N   V N +D S+    D     E   E       ES +    +
Sbjct: 552 DFNRQL-VSEPVE--ANPIDVVNNEDSSIRETEDINTDFETAAE-------ESIVMEENS 611

Query: 630 SSIFSDPTSSFSGVNDNENDLNVSGRS--------------------SVASNLSLD---- 689
           S +FSDP S     N  END + S  S                    S++S  S++    
Sbjct: 612 SDLFSDPNSPLRDSNYIENDSDSSNESNLFPDETVKDRETSVVDSPLSISSQPSMEFIVS 671

Query: 690 -ENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRK--LLGKFQWFWKF 749
              D  T  +   LP S    +E    +S   +++   +    K+R   L GKFQWFWKF
Sbjct: 672 LSKDQETSVVDSPLPVSSQPSIEFPVTQSNDEDNAADKSVANIKERSKVLPGKFQWFWKF 731

Query: 750 GRNVVSEGK--GGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKN 809
           GRNV +E     G E+SKS  + S+ + ++       P++ S  SKGD  DQNVM TLKN
Sbjct: 732 GRNVTAEETRCNGVESSKSDLVCSSESHSL-------PQASSSGSKGD-TDQNVMNTLKN 791

Query: 810 LGHSMLEHVQVIETVFQQERGQV--GSLENLSKNPLVGKGQVTAMAALKELRKISNLLSE 833
           LG+SMLEH+QVIE+VFQQERGQV  G +ENLSKN LV KGQVTAM ALKELRKISNLL E
Sbjct: 792 LGNSMLEHIQVIESVFQQERGQVQAGLIENLSKNNLVEKGQVTAMTALKELRKISNLLLE 828

BLAST of MC08g2074 vs. TAIR 10
Match: AT2G19240.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 662.9 bits (1709), Expect = 3.3e-190
Identity = 430/877 (49.03%), Postives = 541/877 (61.69%), Query Frame = 0

Query: 1   MASSEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVLPSSSLASIDD 60
           MA SEI  AL EP       SGS+    S + RRF +LRGVRWR+NLGVLPS + +SID+
Sbjct: 1   MAPSEIEPALQEP-------SGSLV--LSREDRRFANLRGVRWRVNLGVLPSLA-SSIDE 60

Query: 61  LRRVTADSRRRYAILRRRLLVDPHISKDGSNSPDIAIDNPLSQNPDSMWGRFFRSAELEK 120
            RR  A+SRR     RRRLL+DPH+ K   +SP+  IDNPLSQNP+S WG+FFR+AELEK
Sbjct: 61  FRRAAANSRR-----RRRLLMDPHVLKHEDSSPNFIIDNPLSQNPNSTWGQFFRNAELEK 120

Query: 121 MVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVD 180
            +DQDLSRLYPEH  YFQTP  QG+LRRILLLWCL+HP++GYRQGMHELLAPLLYVLHVD
Sbjct: 121 TLDQDLSRLYPEHWCYFQTPRYQGMLRRILLLWCLKHPEYGYRQGMHELLAPLLYVLHVD 180

Query: 181 VERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVHGNEENVKSLSE 240
           + RLS+VRK YED F D FD LSF +      FD    +D+ ++ +G  G+ +N  SL E
Sbjct: 181 IMRLSEVRKSYEDYFTDRFDSLSFMERDITYTFDFNKFMDSVDNGIGSQGHSKNFNSLDE 240

Query: 241 LDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPA 300
           LDPE+Q++++LTD+YG E ELGIVLSE+F+EHDAY MFDALMSG HG  +M  FFS +PA
Sbjct: 241 LDPEVQSLVMLTDSYGTESELGIVLSEKFMEHDAYCMFDALMSGIHGCFAMASFFSYSPA 300

Query: 301 GSSLSGLPPVIEASAALYHLLSHVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDL 360
             S +GL PV+EA +A Y +L+ VD SLH+HLVELGVEPQYF LRWLRVLFGREF L+DL
Sbjct: 301 SGSHTGLTPVLEACSAFYRILAVVDSSLHSHLVELGVEPQYFGLRWLRVLFGREFLLQDL 360

Query: 361 LTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENAT 420
           L +WDEI  +DN    R+DE   + +       RG  +  + VSM+LYLRSSLL+TENAT
Sbjct: 361 LLVWDEIILADNSA--RTDEDSRNQNFRIFDCPRGTLVLGMTVSMILYLRSSLLSTENAT 420

Query: 421 LCLQRLLNFPKNVDLKKLIEKAKSLQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSS 480
            CLQRLLNFP+N+DL K+I+KAK LQ L L +++ S+ S++G +   S  + AR+    S
Sbjct: 421 CCLQRLLNFPENIDLNKIIQKAKLLQALVLDTDMLSALSINGVF-DQSNFVPART---KS 480

Query: 481 GSVSPKTPLSLVPESYWEEKWRVLHR--EEEFKQSGSKNQNAAQKKGWSEKVRFLYRTES 540
            S SP++PL + PESYWE+KWRVLH+  EEE K    K     QKK     V  L+R   
Sbjct: 481 CSTSPRSPLIIAPESYWEKKWRVLHKAEEEENKICLEKQTPPTQKKKRWLNVTKLFRAVI 540

Query: 541 DPSPAKLAGGKKNTKSS-VRRRLLADLSRELGA-------AEDIEKCGNEEVV----DNK 600
           D S  KL  G++   SS V + LL D S +L          E+I     EE +       
Sbjct: 541 DLSHHKLGIGERKANSSPVTQSLLEDSSEQLNVDCHVTVNKENIHPQETEENIMEFHSAD 600

Query: 601 DDLSVEGEVDGQDSCEKCLENAVDKRCESGI-------AGS----------------ENS 660
           ++  V G    ++S    L+     RC + I       AGS                ENS
Sbjct: 601 EESIVSGSSPSEESSFVSLDPTSPVRCSTKIENDSVSSAGSNLLPDEDDKSIVSISEENS 660

Query: 661 SIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDENDDRTHSIVEDLPFSVPEQLENIP 720
           S+ SDP S          D N SG+       S ++ D +T     + P SV     N  
Sbjct: 661 SVSSDPISP-------AIDSNYSGKYLDCCTGSENDKDQQTS---VNSPLSVSPHRRNEY 720

Query: 721 EKSGCNNDSEGNATVG-TKDRKLL-GKFQWFWKFGRNVVSEGKGGTETSKSTGIESNPTK 780
             +  + D   + +VG TK+ KLL G  QWF K  R + SE     ETS     ++  T 
Sbjct: 721 PVTQSDEDVSTDKSVGITKEYKLLHGIVQWFRKLKRTLSSE-----ETSHRKASDATKTN 780

Query: 781 NIASPKID-GPRSIS---VSSKGDGVDQNVMGTLKNLGHSMLEHVQVIETVFQQERGQV- 833
           ++   K   G  S S     S GD   QN+  TLKNLG SML+H++ IE VFQQE   V 
Sbjct: 781 DVKIKKNQIGCYSESHPQALSSGDS-SQNLRKTLKNLGQSMLKHIEAIELVFQQEPCLVP 840

BLAST of MC08g2074 vs. TAIR 10
Match: AT5G57210.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 657.1 bits (1694), Expect = 1.8e-188
Identity = 420/814 (51.60%), Postives = 521/814 (64.00%), Query Frame = 0

Query: 34  RFVDLRGVRWRINLGVLPSSSLASIDDLRRVTADSRRRYAILRRRLLVDPHISKDGSNSP 93
           RF  LRGVRWRINLG+LPSS  ++ID+LRRVTADSRRRYA LRRRLL+DPH+ K G+NSP
Sbjct: 29  RFEKLRGVRWRINLGILPSSPSSTIDELRRVTADSRRRYAALRRRLLIDPHLPKKGTNSP 88

Query: 94  DIAIDNPLSQNPDSMWGRFFRSAELEKMVDQDLSRLYPEHGSYFQTPGCQGLLRRILLLW 153
           D+ IDNPLSQNPDS WGRFFR+AELEK +DQDLSRLYPEHGSYFQ+ GCQG+LRRILLLW
Sbjct: 89  DLTIDNPLSQNPDSTWGRFFRNAELEKTLDQDLSRLYPEHGSYFQSSGCQGMLRRILLLW 148

Query: 154 CLQHPQFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSFQD-NSFKSN 213
           CL+HP+ GYRQGMHELLAPLLYVL VDV+ L++VR  YEDQF D FD L+FQ+ +S   +
Sbjct: 149 CLKHPEIGYRQGMHELLAPLLYVLQVDVQYLTEVRSNYEDQFVDLFDELAFQERDSGAYD 208

Query: 214 FDLKNRLDATEDEVGVHG------NEENVKSLSELDPEIQTIILLTDAYGAEGELGIVLS 273
           FD+K  LD + ++    G       ++  KS  ELD E QT +LL+DAYG EGELGIVLS
Sbjct: 209 FDIKKVLDDSMEDEEEDGPPSGSTKKKKPKSFDELDTETQTAVLLSDAYGGEGELGIVLS 268

Query: 274 ERFIEHDAYAMFDALMSGAH--GSVSMGDFFSPTPAGSSLSGLPPVIEASAALYHLLSHV 333
           ++F+EHDAY MFDALM G    GSVS+ +FF  +    S++GLPPVIEAS ALYHLLS V
Sbjct: 269 DKFMEHDAYTMFDALMYGGSSLGSVSVANFFIYSAPNDSITGLPPVIEASGALYHLLSLV 328

Query: 334 DLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDLLTIWDEIFASDNGKFYRSDEPETS 393
           D SLH+HLVELGVEPQYF+LRWLRVLFGREF L +LL +WDEIF++DN +  R  E +  
Sbjct: 329 DASLHSHLVELGVEPQYFALRWLRVLFGREFPLSNLLIVWDEIFSADNSEVERGVEADLG 388

Query: 394 SSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKNVDLKKLIEKAKS 453
                LSS RGA +A +AVSM+LYLRSSLLATENAT  L++LLNFP+++DL K+IEKAK+
Sbjct: 389 CEFRILSSPRGALVAGMAVSMILYLRSSLLATENATSSLKKLLNFPEDIDLSKVIEKAKT 448

Query: 454 LQTLALQSNISSSPSLSGAYHHHSKSMVARSNGLSSGSVS-PKTPLSLVPESYWEEKWRV 513
           LQ+LAL+ N        G      K M  R + LS  S+S   +P+ + PESYWEEKWRV
Sbjct: 449 LQSLALEINARRDLIPKGP----RKPM--RGHSLSVDSISLGSSPVGIEPESYWEEKWRV 508

Query: 514 LH-REEEFKQSGSKNQNAAQKKGWSEKVRF-LYRTESDPSPAKLAGGKKNTKSSVRRRLL 573
           L+  EEE ++  +  +  A KK WSE+V+  L RTESDPSPA+    +   K  +RR LL
Sbjct: 509 LNSAEEEERRKKALQRPKAGKKSWSERVKLRLSRTESDPSPAE--AKRSGNKPHIRRSLL 568

Query: 574 ADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDSCEKCLENAVDKRCESGIAGSE 633
            DLSR+LG                                EK +E       ++ I   E
Sbjct: 569 DDLSRQLG--------------------------------EKEIEPPEFPNPDTDI---E 628

Query: 634 NSSIFSDPTSSFSGVNDNENDLNVSGRSSVASNLSLDENDDRTHSIVEDLPFSVPEQLEN 693
            SS  SD  S  +   DN +D    G+S         EN         DLP S+P   EN
Sbjct: 629 RSSTVSDTPS--AEYEDNSSD---KGKS---------ENH-------MDLPLSIP---EN 688

Query: 694 IPE-KSGCNNDSEGNATVGTKDRKLL-GKFQWFWKFGRNVVSEGKGGTETSKSTGIESNP 753
            PE KSG N           ++RK+L GKFQ  W+ GRN+  E    T+ +K    E   
Sbjct: 689 EPEAKSGMNI---------FRERKILSGKFQRLWRLGRNLSGEETSETKEAKQVDSEDGK 737

Query: 754 TKNIASPKIDGPRSISVSSKGDGVDQNVMGTLKNLGHSMLEHVQVIETVFQQERGQVGSL 813
           T            S S S  GD        +LKN G SMLEH++VIE+V      ++ S 
Sbjct: 749 TD-----------SDSTSGPGD--------SLKNTGRSMLEHIKVIESVL-----ELSSP 737

Query: 814 ENLSKNPLVGKGQVTAMAALKELRKISN-LLSEM 833
           EN+++N     G++T   AL+ELR++ N LLSEM
Sbjct: 809 ENMTEN-----GKLTVEEALRELRRLGNMLLSEM 737

BLAST of MC08g2074 vs. TAIR 10
Match: AT4G29950.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 617.1 bits (1590), Expect = 2.1e-176
Identity = 387/726 (53.31%), Postives = 484/726 (66.67%), Query Frame = 0

Query: 145 LLRRILLLWCLQHPQFGYRQGMHELLAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSF 204
           +LRRILLLWCL+HP++GYRQGMHELLAPLLYVLHVDV+RLS+VRK YED F D FDGLSF
Sbjct: 1   MLRRILLLWCLKHPEYGYRQGMHELLAPLLYVLHVDVDRLSEVRKSYEDHFIDRFDGLSF 60

Query: 205 QDNSFKSNFDLKNRL-DATEDEV-GVHGNEENVKSLSELDPEIQTIILLTDAYGAEGELG 264
           ++     NF+ K  L D T+DE+ G+ GN + +KSL ELDPEIQ+I+ L+DAYGAEGELG
Sbjct: 61  EERDITYNFEFKKFLEDFTDDEIGGIQGNSKKIKSLDELDPEIQSIVRLSDAYGAEGELG 120

Query: 265 IVLSERFIEHDAYAMFDALMSGAHGSVSMGDFFSPTPAGSSLSGLPPVIEASAALYHLLS 324
           IVLSE+F+EHDAY MFDALM+G HG V+M  FF+ +PA  S +GLPPV+EAS A YHLLS
Sbjct: 121 IVLSEKFMEHDAYCMFDALMNGVHGCVAMAGFFAYSPASGSHTGLPPVLEASTAFYHLLS 180

Query: 325 HVDLSLHAHLVELGVEPQYFSLRWLRVLFGREFSLEDLLTIWDEIFASDNGKFYRSDEPE 384
            VD SLH+HLVELGVEPQYF LRWLRVLFGREF L+DLL +WDEIF++DN     +D   
Sbjct: 181 FVDSSLHSHLVELGVEPQYFGLRWLRVLFGREFLLQDLLIVWDEIFSADNTTRTDADN-N 240

Query: 385 TSSSSGFLSSSRGAFIAAIAVSMLLYLRSSLLATENATLCLQRLLNFPKNVDLKKLIEKA 444
           T+ S     S RGA I+ +AVSM+L LRSSLLATENA  CLQRLLNFP+ +D++K+IEKA
Sbjct: 241 TNQSYKIFDSPRGALISGMAVSMILCLRSSLLATENAASCLQRLLNFPEKIDVRKIIEKA 300

Query: 445 KSLQTLALQSNI-SSSPSLSGAYHHH-SKSMVARSNGLSSGSVSPKTPLSLVPESYWEEK 504
           KSLQTLAL  ++ SS+ S++  +    S ++ AR+N   SGS SPK+PL + P+SYWE++
Sbjct: 301 KSLQTLALDDDVRSSALSINDGFDQSISPAVPARTNSFPSGSTSPKSPLIITPQSYWEDQ 360

Query: 505 WRVLHR-EEEFKQSGSKNQNAAQKKGWSEKVRFLYRTESDPS-PAKLAGGKKNTK-SSVR 564
           WRVLH+  EE K+S S  Q   +KK W  +V+ L+R ES+P+  AK   GK   K SSV 
Sbjct: 361 WRVLHKAAEEEKKSPSPIQ---KKKPWF-RVKRLFRAESEPTHSAKSPNGKSEVKISSVA 420

Query: 565 RRLLADLSRELGAAEDIEKCGNEEVVDNKDDLSVEGEVDGQDSCEKCLENAVDKRCESGI 624
           R LL D +R+L  +E +E   N   V N +D S+    D     E   E       ES +
Sbjct: 421 RNLLEDFNRQL-VSEPVE--ANPIDVVNNEDSSIRETEDINTDFETAAE-------ESIV 480

Query: 625 AGSENSSIFSDPTSSFSGVNDNENDLNVSGRS--------------------SVASNLSL 684
               +S +FSDP S     N  END + S  S                    S++S  S+
Sbjct: 481 MEENSSDLFSDPNSPLRDSNYIENDSDSSNESNLFPDETVKDRETSVVDSPLSISSQPSM 540

Query: 685 D-----ENDDRTHSIVEDLPFSVPEQLENIPEKSGCNNDSEGNATVGTKDRK--LLGKFQ 744
           +       D  T  +   LP S    +E    +S   +++   +    K+R   L GKFQ
Sbjct: 541 EFIVSLSKDQETSVVDSPLPVSSQPSIEFPVTQSNDEDNAADKSVANIKERSKVLPGKFQ 600

Query: 745 WFWKFGRNVVSEGK--GGTETSKSTGIESNPTKNIASPKIDGPRSISVSSKGDGVDQNVM 804
           WFWKFGRNV +E     G E+SKS  + S+ + ++       P++ S  SKGD  DQNVM
Sbjct: 601 WFWKFGRNVTAEETRCNGVESSKSDLVCSSESHSL-------PQASSSGSKGD-TDQNVM 660

Query: 805 GTLKNLGHSMLEHVQVIETVFQQERGQV--GSLENLSKNPLVGKGQVTAMAALKELRKIS 833
            TLKNLG+SMLEH+QVIE+VFQQERGQV  G +ENLSKN LV KGQVTAM ALKELRKIS
Sbjct: 661 NTLKNLGNSMLEHIQVIESVFQQERGQVQAGLIENLSKNNLVEKGQVTAMTALKELRKIS 703

BLAST of MC08g2074 vs. TAIR 10
Match: AT4G13730.3 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.8e-10
Identity = 104/459 (22.66%), Postives = 169/459 (36.82%), Query Frame = 0

Query: 4   SEIVAALSEPASATSSSSGSVCHGNSEDKRRFVDLRGVRWRINLGVL-PSSSLASIDDLR 63
           +++VA LS+              G  +D      +R + W++ L  L P  SL S +   
Sbjct: 101 AQVVAELSKKVIDLKELRKIASQGLPDD----AGIRSIVWKLLLDYLSPDRSLWSSE--- 160

Query: 64  RVTADSRRRYAILRRRLLV-------DPHISKDG--SNSPDIAIDNPLSQNPDSMWGRFF 123
              A  R +Y   +  LL+       DP I   G  S S     D+PLS    S+W  FF
Sbjct: 161 --LAKKRSQYKQFKEELLMNPGGDSNDPKIESPGALSRSEITHEDHPLSLGTTSLWNNFF 220

Query: 124 RSAELEKMVDQDLSRLYPE----HGSYFQTPGCQGLLRRILLLWCLQHPQFGYRQGMHEL 183
           +  E+ + +++D+ R +P+     G        Q  L+ IL ++   +P   Y QGM+E+
Sbjct: 221 KDTEVLEQIERDVMRTHPDMHFFSGDSAVAKSNQDALKNILTIFAKLNPGIRYVQGMNEI 280

Query: 184 LAPLLYVLHVDVERLSQVRKLYEDQFADNFDGLSFQDNSFKSNFDLKNRLDATEDEVGVH 243
           LAP+ Y+                                FK++ D  N            
Sbjct: 281 LAPIFYI--------------------------------FKNDPDKGN------------ 340

Query: 244 GNEENVKSLSELDPEIQTIILLTDAYGAEGELGIVLSERFIEHDAYAMFDALMSGAHGSV 303
                                                  + E DA+  F  LMSG   + 
Sbjct: 341 -------------------------------------AAYAESDAFFCFVELMSGFRDNF 400

Query: 304 SMGDFFSPTPAGSSLSGLPPVIEASAALYHLLSHVDLSLHAHL-VELGVEPQYFSLRWLR 363
                       +S+ G+   I     L  LL H D  L  HL V   + PQ+++ RW+ 
Sbjct: 401 CQ-------QLDNSVVGIRYTI---TRLSLLLKHHDEELWRHLEVTTKINPQFYAFRWIT 437

Query: 364 VLFGREFSLEDLLTIWDEIFASDNGKFYRSDEPETSSSSGFLSSSRGAFIAAIAVSMLLY 423
           +L  +EF+  + L IWD + +   G       P+ +             +  I  +ML+ 
Sbjct: 461 LLLTQEFNFVESLHIWDTLLSDPEG-------PQET-------------LLRICCAMLIL 437

Query: 424 LRSSLLATENATLCLQRLLNFPKNVDLKKLIEKAKSLQT 448
           +R  LLA +  T  L+ L N+P   ++  ++  A  L+T
Sbjct: 521 VRRRLLAGD-FTSNLKLLQNYPP-TNISHMLYVADKLRT 437