MC08g2021 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g2021
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAuxin efflux carrier component
LocationMC08: 28706903 .. 28712033 (-)
RNA-Seq ExpressionMC08g2021
SyntenyMC08g2021
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACAGCTAATTCCTATTTCGCCATGATCGGGTGGGAAGATGCTTATAAGGTGGTGGCCGCCATGGCGCCATTGTACTTCGCCTTGGCGCTTGGCTATGGCTCTGTGAGGTGGTGGAAAATCTTCAGCACTGAACAGTGCGACGCCATTAACAAACTCGTCTGTTACTTCACTCTCCCTCTCTTCACCTTCGACTTCACTTCCCACATCGACCCTTTTCACCTGAACTTCCCTTTCATTGCCGCCGACGCCATCTCGAAGCTCCTCATCGTCGTCGTTTTGGCCTTCTGGGCCAGATGCACCCCCAAAGGGAGCTATTGTTGGTCCATCACTAGCTTCTCCCTCTCCACTCTCACGAACTCGCTCGTTGTTGGCGTCCCGCTCGCCAAGATCATGTATGGTCAGATGGCCGTGGACCTCGTCGTGCAGGCAATATTTTAAATATTGATGCAGATGAAAATGTTGAAGTTTCGTAAATGATATTTGTAAAAATGTTTTAATCATTTCACACTTTTTTTTTAGGAAATACTTAGATTGCTAATTGGTGAGTACCTGTGTTGACTATTGGTATTTTTGACAAATAAGAGAATGGTCAATCTATATAGCACCTAAATATATTTTTTTAATAAATTAAAATTTTTTTCACCTCTAAGATCTTTTTACAAGTGCTTGTTTGTTTTATTATTGGATAAAAAAATAGTAAATTTGTCTTCAAGGTGCTGTTCACATTAGTGATATTATGTTAACGATTTCTGAGTTTTGTTGATATTTTTATGGTAGTATAGAAATATTGATTAACTTTTCATATAAATGTCGAATTTATATATATGTAAAAATGTTGACATGTCAATGGTTTTTTAAAATCTTTGATGCAGGGTTCAGTGGTTCAGGCCATTGTTTGGCTGACAATACTGTTATTTGTGCTGGAACTTCGACGAACGAGGTTGGATTTGGTTGCGGGGGAAGCTTCTTCGAGCTTCGGCCCCGGGGACCATGGGAAAGCTCTACAAATGGTGGAAGAGGGAAGGAAGAATTTGGAGGGAGAGGCCATGGAGGTTGAGGGAAGAGAAACAAGCAACAGCCATAGGGCTTCTTTCAAGCCTTTGATGAAGAAAGTTTGGCTCAAATTGGCTGCAAATCCCAACTCTTATGCTTGTATCATTGGTTTTGCTTGGGCATTTGTTGCAAAAAGGTACTTTCATATTTCAAAATTTTCAAAAAAAAAAAAAAAAAGAATTTCATTTTTATCCTATATTCCAGTCTCTAAACTTCCAAACATTTCATCGAGTTTTCGACTTTTAAATATTATATTTTAGTTCTTAATCTTTACATTAAAGATTATTTTATTTTAATTTTTGAATTTTATTATAAACTATTCGACAGGAATCTCAAATATACTTTTAAAACTTGACATCTATTATTACCGCATGTGATTTTAATGTTTATTTTATATTTATAAAGTAAATGAACAAAACACATATTATGATTGAAATTTGCGTTAAATAATTTATTAAATTAATGCCAAGGACTAAAATGGTATTTTACGTAAAATTTAAGAACTAAATTATATAGTATTTTTAAAGTTTAGAGACATTAAATTAAATAGAACAAACATATAATCAGTTCAGACCAAAATAAAATTTAAACCATGATTATAATGTTTTTTTTTATATTTAGTTTACAATTTATAATGTTTGTTATTATACAAATATGTTTTGAAATTATGTATAGGTGGCATATTGAAATGCCTAGCATTATGGAAGGATCTATTTTAATCATGTCAAAAGCTGGGATTGGCACTGCCATGTTTAATATGGGTAAGATTACACAATTCTTCTTTTAATTTTTGTTTTGCAAAGTTTTTAATCGTTATAAATATATGAGAAAAATTTTCTTCTAAAACAGCAAAATATTAATTAATATTTAATACTATTTATCTTTTGAAGTCTCAAATTGATTACAAATAAATTAGTGTCCCTTAAAAGAGAGAATTTTTAATAATCAAACAATCTAAAATTTGATTTTGACTTGTCATATTAATTCTTCATAAAGTTTTTAAACCATACAGTATTAGTTAAATTTCTAATTGTCCAAATAATCCCCCAATAAGAGTCTTTAACAATCTCATTTTTTTTTTGTCTCGAAAAAGAAAAAAAAAATAGAAAAACAATCTGTGGATTAGTGGGTCTAATAGAAGATTTGAGTGGGAATCTATTCAACATAAATATGTCACAAGCTTAAACAAATCAATGTAAAAAAAATAATATAAATGGTTTTTGTTATTATTATTATTTTTTTTTAGTAAAACATGTTGAGGAGATTGAAGGTATATGCTAGTTACGAGCTAGGCTCGTTTTGACGGTTTTTGTTAAGTTTATTTAAACCAAAATAGTAAAATTAAGTTTAATATATAAGTTCCATATTTCATTCTCTTAATTAGTACTTTAAGCATTACACATCTAGTTTATTTGAATAATTATTCTTACCTTTAATGTCTATTATTAACATCCACCTAACAAAATCATCGACGTGACACTCAAAAATTAAAGTTTGGATATGAAACTAATTAAAAATTAGGAGATATATAAGAATAATATTGATTGATTGTGAGAAAGAACACAAATACAAATACGCTATCTAAAATTTAAGAATAGAGTACCATGTGGTCCATTCAACGGCCAATTCTAAATATACACCTACCTTATCTAAGTTTCATACGTCTCTATCTTTTTCGTTAACTAATCATGTTAACAACAAAGATCAAAAGTCGCTAATTTTAGACATCTATCTCCGATTAAAAGGACGTGAGTTCAAATCTCCACTCCTACATATTGTACCAAAAAATAAAGATCAAACGTAAAACGTAAACTTAATTCAAAGTATACGGATTTTCTACGTTTTCGTCGCTGAAGTCCTCTAGCGACCCAGTTCTAGCAATTGGGCTGGTGATAATATGTTTTCGTCACTGAATACCTTCAGCGACAAAAATCTCGCTTCAGTGACGAAATATTTCGTCGTTGAAGACATTTTTTCTTGTAGCATAGGGCGAATCATATTTCTTATGGATTTCTTTTAAGTGCATGCAGGAATCTTCATGGCACTGCAAGAGAAGTTGATAGCCTGTGGAGCTAGCCTTACCATAATAGGAATGGTTTTAAAGTTCATTGCTGGACCAGCTGCCATGGCTATTGGCTCCATGGCCATGGGCTTGCATGGTGATGTTCTACGCGTAGCTATCATCCAGGTAAAAAAAAAAAAAAAAGAATTAACAGAACTTTTGAATTGGACAGAACTCGTGAATATTGATACGACCTATCAGATCACACCAAATTAATGACCTAAATTTACTTAAGGTTTAAATCTTATAATCATGGTTTCTGGACGACTTTTATAATTATTATATTTTCGTTTTCTAAACTTTTATGTGATTTATGTTTTAGTCTCGTGGTCCATATATAGTCTTTTTTTAGTAAAATCGATTACTCAACAAAACCCTAAATCTAATTGGAAATATATAATATTTTAAAAACTATATATGTGACTTTAAATACTTGCCGATGTAGACATCTATCGATAAAGTTAATCAACAAAAATGTGTTGATGTTAGTAACCAAAATAGAATTAAATTATTATTGCTATCATTTTGGAATACAACATTACACAAACAAATTTTTGGGTGAGAATTCGACCTTCGACTTTAAGAGATTGTTATTAATGCTTTAATTACCATTAGCTAATCGCTAAAGTTAGAAAATTTGAGCTTTCTTTTTGTTTTCATAAAAGAAGATTTGAACTTTTTTGAATGAGCATATTTTTGTTATTGGTATTAATAATAAACAGAATTAAGCATAATCCAAAAGTTTAGTGTGTTTAATATACAATGCTTTAATATTTGTTGCAGGCAGCAGTACCACAGTCTATTACATCATTTATTTATGCCAAAGAATATGGCTTACATGCAGAAGTTCTTAGCACAGCGTAAGAATAATTACTTATTTTTCTTTCAAGTTTTTTTTTTTTAAATATGTTTGTTCCCTTGTAAAAAAAAAATATATTTATATTTTAGATATTATTATATAACAGGGGAAATTATTTCAAATGGAATTTTAATTTATCACAAAAAAAGTGGGTATAATTTGTCTCTAGCGAACATTTGACTCATAGGTTTTTGTTTTAAAAAAATGGGTTTCAATAATAAAAATCTAACAAAAGAAAAAAAAAGGAATTGTTAGTAAGCAAACCCTAATTAACAACATAAACTGAAGGCTAACATTCTATCGAGTGGAAAATAATCAGTATTAAAAATTTAATAAAAATAGAAAATAGAAAATAAAAATATTATCAAACAAGCAAGTTATTTTTTTCCAATCCAAAATTACTAAATACTCTTAAAAAGAATTAGTAGATAAGCTATAAATCTTATTAATTATTAGTATTTGACAATATTTTTTATTGATCATCTTCCTTTAACGGACTGGAACTAGAATCAAAGTATTAGTAGATTTGTTCAGTTCAAAATGGTAGCGGACGAACTTGACCATTAATATCACTTTGCCAAAATTTTCATTTATAAAAATATTAGAATGATTATAATATACATATATATTTTAATGCAGGGTGATTTTTGGGGCAATAGTTTCTCTTCCTGTGTTGGTTGCTTATTATGCAGCATTAGAGTTTGTACATTGACCCTTTTACGAGGTCTCATCCCCACATTTTTAAAGAATGAGAAAAAGGGAAGGTTAGAATTGGATTATTAGGACTCCATTTTAATTAATTAGTTCACTTAACTTTTTTTTTTTTCATTACTTTTTTTTTTTCATTTCTATTTATAAGTGTAATTTTGTTGGAGGTTTCTAGAGAAAATGTCTAAAGATGAAAATATTATTTTATCAGAGCCAGGTTTCAATCTTGGGACCTGTGAGTTATGGGCCCACCACACTTCCGCTGCGCCACTCTGATTTGTTGGTATAATAAGATCATAATTTTGAAAACTATGCTGAGCAAACATAGGTGAAAAATCAAACCGACGAATCGAAACCGACCAAATCCAAGTTGGTCAGTTGTTGTAAGTGGTCTATGGTAGGTGTTTCGGTTCACCTAGACCCCTAAAACTGATCAACCGACCGACC

mRNA sequence

ACACAGCTAATTCCTATTTCGCCATGATCGGGTGGGAAGATGCTTATAAGGTGGTGGCCGCCATGGCGCCATTGTACTTCGCCTTGGCGCTTGGCTATGGCTCTGTGAGGTGGTGGAAAATCTTCAGCACTGAACAGTGCGACGCCATTAACAAACTCGTCTGTTACTTCACTCTCCCTCTCTTCACCTTCGACTTCACTTCCCACATCGACCCTTTTCACCTGAACTTCCCTTTCATTGCCGCCGACGCCATCTCGAAGCTCCTCATCGTCGTCGTTTTGGCCTTCTGGGCCAGATGCACCCCCAAAGGGAGCTATTGTTGGTCCATCACTAGCTTCTCCCTCTCCACTCTCACGAACTCGCTCGTTGTTGGCGTCCCGCTCGCCAAGATCATGTATGGTCAGATGGCCGTGGACCTCGTCGTGCAGGGTTCAGTGGTTCAGGCCATTGTTTGGCTGACAATACTGTTATTTGTGCTGGAACTTCGACGAACGAGGTTGGATTTGGTTGCGGGGGAAGCTTCTTCGAGCTTCGGCCCCGGGGACCATGGGAAAGCTCTACAAATGGTGGAAGAGGGAAGGAAGAATTTGGAGGGAGAGGCCATGGAGGTTGAGGGAAGAGAAACAAGCAACAGCCATAGGGCTTCTTTCAAGCCTTTGATGAAGAAAGTTTGGCTCAAATTGGCTGCAAATCCCAACTCTTATGCTTGTATCATTGGTTTTGCTTGGGCATTTGTTGCAAAAAGGTGGCATATTGAAATGCCTAGCATTATGGAAGGATCTATTTTAATCATGTCAAAAGCTGGGATTGGCACTGCCATGTTTAATATGGGAATCTTCATGGCACTGCAAGAGAAGTTGATAGCCTGTGGAGCTAGCCTTACCATAATAGGAATGGTTTTAAAGTTCATTGCTGGACCAGCTGCCATGGCTATTGGCTCCATGGCCATGGGCTTGCATGGTGATGTTCTACGCGTAGCTATCATCCAGGCAGCAGTACCACAGTCTATTACATCATTTATTTATGCCAAAGAATATGGCTTACATGCAGAAGTTCTTAGCACAGCGGTGATTTTTGGGGCAATAGTTTCTCTTCCTGTGTTGGTTGCTTATTATGCAGCATTAGAGTTTGTACATTGACCCTTTTACGAGGTCTCATCCCCACATTTTTAAAGAATGAGAAAAAGGGAAGAGCCAGGTTTCAATCTTGGGACCTGTGAGTTATGGGCCCACCACACTTCCGCTGCGCCACTCTGATTTGTTGGTATAATAAGATCATAATTTTGAAAACTATGCTGAGCAAACATAGGTGAAAAATCAAACCGACGAATCGAAACCGACCAAATCCAAGTTGGTCAGTTGTTGTAAGTGGTCTATGGTAGGTGTTTCGGTTCACCTAGACCCCTAAAACTGATCAACCGACCGACC

Coding sequence (CDS)

ATGATCGGGTGGGAAGATGCTTATAAGGTGGTGGCCGCCATGGCGCCATTGTACTTCGCCTTGGCGCTTGGCTATGGCTCTGTGAGGTGGTGGAAAATCTTCAGCACTGAACAGTGCGACGCCATTAACAAACTCGTCTGTTACTTCACTCTCCCTCTCTTCACCTTCGACTTCACTTCCCACATCGACCCTTTTCACCTGAACTTCCCTTTCATTGCCGCCGACGCCATCTCGAAGCTCCTCATCGTCGTCGTTTTGGCCTTCTGGGCCAGATGCACCCCCAAAGGGAGCTATTGTTGGTCCATCACTAGCTTCTCCCTCTCCACTCTCACGAACTCGCTCGTTGTTGGCGTCCCGCTCGCCAAGATCATGTATGGTCAGATGGCCGTGGACCTCGTCGTGCAGGGTTCAGTGGTTCAGGCCATTGTTTGGCTGACAATACTGTTATTTGTGCTGGAACTTCGACGAACGAGGTTGGATTTGGTTGCGGGGGAAGCTTCTTCGAGCTTCGGCCCCGGGGACCATGGGAAAGCTCTACAAATGGTGGAAGAGGGAAGGAAGAATTTGGAGGGAGAGGCCATGGAGGTTGAGGGAAGAGAAACAAGCAACAGCCATAGGGCTTCTTTCAAGCCTTTGATGAAGAAAGTTTGGCTCAAATTGGCTGCAAATCCCAACTCTTATGCTTGTATCATTGGTTTTGCTTGGGCATTTGTTGCAAAAAGGTGGCATATTGAAATGCCTAGCATTATGGAAGGATCTATTTTAATCATGTCAAAAGCTGGGATTGGCACTGCCATGTTTAATATGGGAATCTTCATGGCACTGCAAGAGAAGTTGATAGCCTGTGGAGCTAGCCTTACCATAATAGGAATGGTTTTAAAGTTCATTGCTGGACCAGCTGCCATGGCTATTGGCTCCATGGCCATGGGCTTGCATGGTGATGTTCTACGCGTAGCTATCATCCAGGCAGCAGTACCACAGTCTATTACATCATTTATTTATGCCAAAGAATATGGCTTACATGCAGAAGTTCTTAGCACAGCGGTGATTTTTGGGGCAATAGTTTCTCTTCCTGTGTTGGTTGCTTATTATGCAGCATTAGAGTTTGTACATTGA

Protein sequence

MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTSHIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPLAKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQMVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAKRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPAAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVLVAYYAALEFVH
Homology
BLAST of MC08g2021 vs. ExPASy Swiss-Prot
Match: Q9FFD0 (Auxin efflux carrier component 5 OS=Arabidopsis thaliana OX=3702 GN=PIN5 PE=2 SV=2)

HSP 1 Score: 488.0 bits (1255), Expect = 9.2e-137
Identity = 257/371 (69.27%), Postives = 298/371 (80.32%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI   D YKV+ AM PLY AL LGYGSV+WW IF+ +QCDAIN+LVCYFTLPLFT +FT+
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           H+DPF++N+ FIAAD +SK++IV VLA WA+ + KGSYCWSITSFSL TLTNSLVVGVPL
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK MYGQ AVDLVVQ SV QAIVWLT+LLFVLE R+      AG +S++         + 
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEFRK------AGFSSNN---------IS 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
            V+    N+E        RET      SF  +M  VWLKLA NPN Y+CI+G AWAF++ 
Sbjct: 181 DVQVDNINIESGK-----RETVVVGEKSFLEVMSLVWLKLATNPNCYSCILGIAWAFISN 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWH+E+P I+EGSILIMSKAG GTAMFNMGIFMALQEKLI CG SLT++GMVLKFIAGPA
Sbjct: 241 RWHLELPGILEGSILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+ +GLHGDVLRVAIIQAA+PQSITSFI+AKEYGLHA+VLSTAVIFG +VSLPVL
Sbjct: 301 AMAIGSIVLGLHGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSLPVL 351

Query: 361 VAYYAALEFVH 372
           VAYYAALEF+H
Sbjct: 361 VAYYAALEFIH 351

BLAST of MC08g2021 vs. ExPASy Swiss-Prot
Match: Q5JLM1 (Probable auxin efflux carrier component 5a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5A PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.1e-112
Identity = 223/372 (59.95%), Postives = 271/372 (72.85%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW D YKVVAA  PLYFAL LGYGSVRWW+IF+ EQCDA+N+LV +F LP FTF+FT 
Sbjct: 1   MIGWGDVYKVVAATVPLYFALFLGYGSVRWWRIFTREQCDAVNRLVAFFALPFFTFEFTL 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKG--SYCWSITSFSLSTLTNSLVVGV 120
           H DPF +N+  +AAD ISK +IV V+  WAR   KG  +  WSITSFSLSTLTNSLVVGV
Sbjct: 61  HTDPFQVNYRAVAADVISKAVIVAVIGAWARFMSKGGCAVSWSITSFSLSTLTNSLVVGV 120

Query: 121 PLAKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKA 180
           P+A+ MYG+ A  LVVQ SV QAIVWLT+LLFVLE+R+  + +    A ++   G   +A
Sbjct: 121 PMARAMYGEWAQQLVVQLSVFQAIVWLTLLLFVLEVRKAAIGMYVDGAEAAAAAGKDVEA 180

Query: 181 LQMVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFV 240
                 G     G  +       + + + S   L+K V  KLA NPN+YA  +G  WA +
Sbjct: 181 -----AGAAAAAGTVV-----VAAAAGKPSLWALVKVVAHKLARNPNTYASFVGITWACL 240

Query: 241 AKRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAG 300
           A R HI +PS  EGS+LIMSK+G G AMF+MG+FMA QEK+IACG S   +G+VLKF  G
Sbjct: 241 ANRLHIALPSAFEGSVLIMSKSGTGMAMFSMGLFMAQQEKIIACGTSFAALGLVLKFALG 300

Query: 301 PAAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLP 360
           PAAMAIGS+A+GL GDVLRVAIIQAA+PQSITSFI+AKEYGLHA+VLSTAVIFG +VSLP
Sbjct: 301 PAAMAIGSIAVGLRGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSLP 360

Query: 361 VLVAYYAALEFV 371
           +LV +Y  LE +
Sbjct: 361 LLVGFYIVLELI 362

BLAST of MC08g2021 vs. ExPASy Swiss-Prot
Match: D5A7J3 (Probable auxin efflux carrier component 5b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5B PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.4e-108
Identity = 211/383 (55.09%), Postives = 270/383 (70.50%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW D YKVVAAMAPLYFAL LGYGSVRWW++F+ +QCDA+N+LV  F +P F FDF +
Sbjct: 1   MIGWGDVYKVVAAMAPLYFALGLGYGSVRWWRLFTADQCDAVNRLVACFAVPFFAFDFAA 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWA-----RCTPK--------GSYCWSITSFSL 120
            IDPF L++  +AADA+SKL + + LA  A     RC           G + W IT FSL
Sbjct: 61  RIDPFALSYRVLAADALSKLAVALALAACAAAASTRCCGSGGGKRGGGGGFSWCITGFSL 120

Query: 121 STLTNSLVVGVPLAKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEAS 180
           +TL N+LVVGVPL   MYG+ A DL+VQ SVVQ IV+  +LL   E+RR      A  A+
Sbjct: 121 ATLNNTLVVGVPLLDAMYGKWARDLIVQISVVQTIVYFPLLLLAFEVRR------ATTAA 180

Query: 181 SSFGPGDHGKALQMVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSY 240
           ++  P   G     VE+G               T+ + R S  PL++ VWLK+A NPN Y
Sbjct: 181 AAPPPPPTGTDDDDVEDG----------AAAAATAAAARRSLWPLVRAVWLKVARNPNVY 240

Query: 241 ACIIGFAWAFVAKRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLT 300
           A ++G AWA V  RWH+E PSI+EGS+LIMSK G+G +MF+MG+FMALQ+K+I CGA LT
Sbjct: 241 AGVLGVAWACVTNRWHVETPSIIEGSVLIMSKTGVGLSMFSMGLFMALQDKIIVCGAGLT 300

Query: 301 IIGMVLKFIAGPAAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLST 360
           ++GM L+F+AGPAA A+G+ A+GL GD+LR+AIIQAA+PQSIT+F++AKEYGLHAE+LST
Sbjct: 301 VLGMALRFVAGPAATAVGAFALGLRGDLLRLAIIQAALPQSITTFVFAKEYGLHAEILST 360

Query: 361 AVIFGAIVSLPVLVAYYAALEFV 371
           AVIFG + SLPVL+ YY  L F+
Sbjct: 361 AVIFGTLASLPVLIVYYIVLGFI 367

BLAST of MC08g2021 vs. ExPASy Swiss-Prot
Match: Q6ZIB5 (Probable auxin efflux carrier component 5c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN5C PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.3e-102
Identity = 198/376 (52.66%), Postives = 252/376 (67.02%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW D YKVV AMAPLYFAL LGYGSVRWW+ F+ EQC AIN +V YF++P FTFDF  
Sbjct: 1   MIGWGDVYKVVGAMAPLYFALGLGYGSVRWWRFFTAEQCAAINTMVVYFSMPFFTFDFVV 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWAR------CTPKGSYCWSITSFSLSTLTNSL 120
             DPF +N+  IAADA+SK + +  +A WAR          G+  WSIT FSL+ L N+L
Sbjct: 61  RTDPFAMNYRVIAADAVSKAIAIAAMAAWARTRCGCAAAKAGAQSWSITGFSLAALNNTL 120

Query: 121 VVGVPLAKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGD 180
           VVGVPL   MYG+ A DLVVQ +VVQ++VW  +LL   ELR+      A       G G 
Sbjct: 121 VVGVPLLDAMYGRWAQDLVVQIAVVQSMVWFPLLLMAFELRK------AWVVGGGGGVGP 180

Query: 181 HGKALQMVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFA 240
              +     E + ++E     V         R  F    + V LKLA NPN YA ++G  
Sbjct: 181 AVMSSSSPPEKQSDVEMNGAVVAAPGGGGGVRLPFWATARTVGLKLARNPNVYASVLGVV 240

Query: 241 WAFVAKRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLK 300
           WA +A RWH+ +P I+ GS+ +MS+ G G +MF+MG+FM  QE++IACGA LT +GM L+
Sbjct: 241 WACIAYRWHLSLPGIVTGSLQVMSRTGTGMSMFSMGLFMGQQERVIACGAGLTALGMALR 300

Query: 301 FIAGPAAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAI 360
           F+AGP A  +G+ A+GL GDVL +AIIQAA+PQSI SF++AKEYGLHA+VLSTAVIFG +
Sbjct: 301 FVAGPLATLVGAAALGLRGDVLHLAIIQAALPQSIASFVFAKEYGLHADVLSTAVIFGTL 360

Query: 361 VSLPVLVAYYAALEFV 371
           +SLP+L+AYYA L FV
Sbjct: 361 ISLPILIAYYAVLGFV 370

BLAST of MC08g2021 vs. ExPASy Swiss-Prot
Match: Q9LFP6 (Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 9.1e-68
Identity = 154/376 (40.96%), Postives = 219/376 (58.24%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D Y VV+A  PLY ++ LG+ S R  K+FS EQC  INK V  F++PL +F   S
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLA----FWARCTPKGSYC-WSITSFSLSTLTNSLV 120
             +PF ++   I +D + K L+VVVLA    FW     +G    W IT  S+S L N+L+
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 VGVPLAKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDH 180
           +G+P+   +YG  A  ++ Q  V+Q+++W TILLF+ EL   R  L +  AS      D 
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARA-LPSSGASLEHTGNDQ 180

Query: 181 GKALQMVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMK---KVWLKLAANPNSYACIIG 240
            +A         N+E E  E E  E     R      MK   K W KL  NPN+YA +IG
Sbjct: 181 EEA---------NIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIG 240

Query: 241 FAWAFVAKRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMV 300
             WA +  R    +P +++ SI ++S  G+G AMF++G+FMA Q  +IACG  + II M+
Sbjct: 241 IIWATLHFRLGWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITML 300

Query: 301 LKFIAGPAAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFG 360
           LKF+ GPA M   +  + L   + +VAI+QAA+PQ +  F++AKEY LH E++ST VIFG
Sbjct: 301 LKFVLGPALMIASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFG 360

Query: 361 AIVSLPVLVAYYAALE 369
            +++LP  +AYY  L+
Sbjct: 361 MLIALPTTLAYYFLLD 366

BLAST of MC08g2021 vs. NCBI nr
Match: XP_022149923.1 (auxin efflux carrier component 5 [Momordica charantia])

HSP 1 Score: 715 bits (1846), Expect = 1.44e-259
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ
Sbjct: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK
Sbjct: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA
Sbjct: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL
Sbjct: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360

Query: 361 VAYYAALEFVH 371
           VAYYAALEFVH
Sbjct: 361 VAYYAALEFVH 371

BLAST of MC08g2021 vs. NCBI nr
Match: XP_038889666.1 (auxin efflux carrier component 5 [Benincasa hispida])

HSP 1 Score: 634 bits (1635), Expect = 1.89e-227
Identity = 324/371 (87.33%), Postives = 348/371 (93.80%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWED YKVV+AMAPLYFAL LGYGSV+WWKIFST+QCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDVYKVVSAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFI ADAI KL+IV+VLAFWA+CT KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIVADAIGKLIIVLVLAFWAKCTSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLVVQGSV+QAIVWLT+LLFVLELRRTRLDL+A EAS  F   +  KA+Q
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIVWLTMLLFVLELRRTRLDLIAAEASPGFVSDE--KAVQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           +  EG K+LEGEAM+VE R +SNSHR S KPLMKKVWLKLA NPNSYAC IGFAWAFVAK
Sbjct: 181 LTGEGGKDLEGEAMKVEERRSSNSHRPSLKPLMKKVWLKLAGNPNSYACTIGFAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWH+EMP+IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG +LTIIGMVLKFIAGPA
Sbjct: 241 RWHVEMPNIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHA+VLSTAVIFGAIVSLPVL
Sbjct: 301 AMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPVL 360

Query: 361 VAYYAALEFVH 371
           VAYYAALEF+H
Sbjct: 361 VAYYAALEFIH 369

BLAST of MC08g2021 vs. NCBI nr
Match: XP_022937707.1 (auxin efflux carrier component 5 [Cucurbita moschata])

HSP 1 Score: 621 bits (1601), Expect = 3.08e-222
Identity = 320/370 (86.49%), Postives = 344/370 (92.97%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWED +KV++AMAPLYFAL LGYGSV+WWKIFSTEQCDAINKLVCYFTLPLFTF+FTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFEFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFIAADAISKL+IV+VLAFWA+C+ KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLVVQGSV+QAI+WLT+LLFVLELRRT L+LVA   SSS     HGKA Q
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESSSSDDDGHGKATQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           MV EG K++EG AMEVE R  SNSH+ SFK LMKKVWLKL ANPNSYACIIG AWAFVAK
Sbjct: 181 MVGEGGKDVEGGAMEVEERR-SNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWHIEMP IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGA+LTIIGMVLKFIAGPA
Sbjct: 241 RWHIEMPDIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH EV STAVIFGAI+SLP+L
Sbjct: 301 AMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPIL 360

Query: 361 VAYYAALEFV 370
           VAYYAALEF+
Sbjct: 361 VAYYAALEFI 369

BLAST of MC08g2021 vs. NCBI nr
Match: XP_004152830.1 (auxin efflux carrier component 5 [Cucumis sativus] >KAE8651707.1 hypothetical protein Csa_006543 [Cucumis sativus])

HSP 1 Score: 620 bits (1598), Expect = 8.81e-222
Identity = 322/371 (86.79%), Postives = 344/371 (92.72%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+D YKVVAAMAPLYFAL LGYGSV+WWKIFST+QCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFIAADAI KL+IV+VLAFWA+CT KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRT LDLVA EASS  G     K ++
Sbjct: 121 AKVMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTGLDLVAAEASS--GVVSEEKTVE 180

Query: 181 MVEEGRKNLEGEAMEVEG-RETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVA 240
           +  EG K+LEGE MEVE  R +S SHR S KPLMKKVW+K+A NPNSYAC IGFAWAFVA
Sbjct: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240

Query: 241 KRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGP 300
           KRWH+EMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG +LTI+GMVLKFIAGP
Sbjct: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300

Query: 301 AAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPV 360
           AAMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHA+VLSTAVIFGAIVSLPV
Sbjct: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360

Query: 361 LVAYYAALEFV 370
           LVAYYAALEF+
Sbjct: 361 LVAYYAALEFI 369

BLAST of MC08g2021 vs. NCBI nr
Match: XP_022966048.1 (auxin efflux carrier component 5 [Cucurbita maxima])

HSP 1 Score: 618 bits (1594), Expect = 3.58e-221
Identity = 318/370 (85.95%), Postives = 344/370 (92.97%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWED +KV++AMAPLYFAL LGYGSV+WWKIFSTEQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNF FIAADAISKL+IV+VLAFWA+C+ KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFHFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLVVQGSV+QAI+WLT+LLFVLELRRT L+LVA   S S    +HGKA+Q
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESLSSDDDEHGKAIQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           MV EG K++EG AMEVE R  SNSH+ SFK LMKKVWLKL ANPNSYACIIG AWAFVAK
Sbjct: 181 MVGEGGKDVEGGAMEVEERR-SNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWHIEMP +MEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGA+LTIIGMVLKFIAGPA
Sbjct: 241 RWHIEMPDLMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH EV STAVIFGAI+SLP+L
Sbjct: 301 AMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPIL 360

Query: 361 VAYYAALEFV 370
           VAYYAALEF+
Sbjct: 361 VAYYAALEFI 369

BLAST of MC08g2021 vs. ExPASy TrEMBL
Match: A0A6J1D820 (Auxin efflux carrier component OS=Momordica charantia OX=3673 GN=LOC111018220 PE=3 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 6.97e-260
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ
Sbjct: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK
Sbjct: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA
Sbjct: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL
Sbjct: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360

Query: 361 VAYYAALEFVH 371
           VAYYAALEFVH
Sbjct: 361 VAYYAALEFVH 371

BLAST of MC08g2021 vs. ExPASy TrEMBL
Match: A0A6J1FBZ5 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111444027 PE=3 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 1.49e-222
Identity = 320/370 (86.49%), Postives = 344/370 (92.97%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWED +KV++AMAPLYFAL LGYGSV+WWKIFSTEQCDAINKLVCYFTLPLFTF+FTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFEFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFIAADAISKL+IV+VLAFWA+C+ KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLVVQGSV+QAI+WLT+LLFVLELRRT L+LVA   SSS     HGKA Q
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESSSSDDDGHGKATQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           MV EG K++EG AMEVE R  SNSH+ SFK LMKKVWLKL ANPNSYACIIG AWAFVAK
Sbjct: 181 MVGEGGKDVEGGAMEVEERR-SNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWHIEMP IMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGA+LTIIGMVLKFIAGPA
Sbjct: 241 RWHIEMPDIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH EV STAVIFGAI+SLP+L
Sbjct: 301 AMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPIL 360

Query: 361 VAYYAALEFV 370
           VAYYAALEF+
Sbjct: 361 VAYYAALEFI 369

BLAST of MC08g2021 vs. ExPASy TrEMBL
Match: A0A6J1HQQ5 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465776 PE=3 SV=1)

HSP 1 Score: 618 bits (1594), Expect = 1.74e-221
Identity = 318/370 (85.95%), Postives = 344/370 (92.97%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWED +KV++AMAPLYFAL LGYGSV+WWKIFSTEQCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWEDVFKVLSAMAPLYFALVLGYGSVKWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNF FIAADAISKL+IV+VLAFWA+C+ KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFHFIAADAISKLVIVLVLAFWAKCSSKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLVVQGSV+QAI+WLT+LLFVLELRRT L+LVA   S S    +HGKA+Q
Sbjct: 121 AKVMYGQMAVDLVVQGSVIQAIIWLTMLLFVLELRRTGLNLVASRESLSSDDDEHGKAIQ 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
           MV EG K++EG AMEVE R  SNSH+ SFK LMKKVWLKL ANPNSYACIIG AWAFVAK
Sbjct: 181 MVGEGGKDVEGGAMEVEERR-SNSHKPSFKLLMKKVWLKLVANPNSYACIIGLAWAFVAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWHIEMP +MEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGA+LTIIGMVLKFIAGPA
Sbjct: 241 RWHIEMPDLMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGATLTIIGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLH EV STAVIFGAI+SLP+L
Sbjct: 301 AMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHPEVFSTAVIFGAIISLPIL 360

Query: 361 VAYYAALEFV 370
           VAYYAALEF+
Sbjct: 361 VAYYAALEFI 369

BLAST of MC08g2021 vs. ExPASy TrEMBL
Match: A0A0A0LH23 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_2G074170 PE=3 SV=1)

HSP 1 Score: 611 bits (1576), Expect = 8.89e-219
Identity = 320/371 (86.25%), Postives = 342/371 (92.18%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGW+D YKVVAAMAPLYFAL LGYGSV+WWKIFST+QCDAINKLVCYFTLPLFTFDFTS
Sbjct: 1   MIGWQDVYKVVAAMAPLYFALILGYGSVKWWKIFSTQQCDAINKLVCYFTLPLFTFDFTS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           HIDPFHLNFPFIAADAI KL+IV+VLAFWA+CT KGSYCWSITSFSLSTLTN+LV+GVPL
Sbjct: 61  HIDPFHLNFPFIAADAIGKLIIVLVLAFWAKCTTKGSYCWSITSFSLSTLTNALVIGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK+MYGQMAVDLV  GSVVQAIVWLTILLFVLELRRT LDLVA EASS  G     K ++
Sbjct: 121 AKVMYGQMAVDLV--GSVVQAIVWLTILLFVLELRRTGLDLVAAEASS--GVVSEEKTVE 180

Query: 181 MVEEGRKNLEGEAMEVEG-RETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVA 240
           +  EG K+LEGE MEVE  R +S SHR S KPLMKKVW+K+A NPNSYAC IGFAWAFVA
Sbjct: 181 VGGEGEKDLEGEGMEVEEMRRSSKSHRPSLKPLMKKVWVKMAGNPNSYACTIGFAWAFVA 240

Query: 241 KRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGP 300
           KRWH+EMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACG +LTI+GMVLKFIAGP
Sbjct: 241 KRWHVEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGPTLTIVGMVLKFIAGP 300

Query: 301 AAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPV 360
           AAMAIGS+AMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHA+VLSTAVIFGAIVSLPV
Sbjct: 301 AAMAIGSIAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHADVLSTAVIFGAIVSLPV 360

Query: 361 LVAYYAALEFV 370
           LVAYYAALEF+
Sbjct: 361 LVAYYAALEFI 367

BLAST of MC08g2021 vs. ExPASy TrEMBL
Match: A0A2C9VF50 (Auxin efflux carrier component OS=Manihot esculenta OX=3983 GN=MANES_08G104400 PE=3 SV=1)

HSP 1 Score: 521 bits (1342), Expect = 2.15e-183
Identity = 274/371 (73.85%), Postives = 310/371 (83.56%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MIGWED YKVV AM PLY AL LGYGSVRWWKIF+ EQ  A+N+ VCYFTLPLFTF+FT+
Sbjct: 1   MIGWEDVYKVVVAMVPLYVALVLGYGSVRWWKIFTPEQSGAVNRFVCYFTLPLFTFEFTA 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           H+DPF++N+ FI ADAISK +IVVVLAFWA+ + KGSY WSITSFSL TLTNSLVVGVPL
Sbjct: 61  HVDPFNMNYLFIGADAISKFIIVVVLAFWAKFSSKGSYTWSITSFSLCTLTNSLVVGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK MYGQMAVDLVVQ SV+QAI+WLTILLFVLE+RRT LD+ +           +G+ L 
Sbjct: 121 AKAMYGQMAVDLVVQSSVIQAIIWLTILLFVLEIRRTGLDISSNT---------NGENLD 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
                 K+LEG +  V+ +    S + SF  LMK VWLK+A NPNSYACIIG  WAF+AK
Sbjct: 181 ------KDLEGISRSVDHK--MESAKPSFWALMKVVWLKMAMNPNSYACIIGLVWAFIAK 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWH EMPSIMEGSILIMS+AG GTAMF+MGIFMALQEKLIACG  LT++GMVL+FIAGPA
Sbjct: 241 RWHFEMPSIMEGSILIMSRAGTGTAMFSMGIFMALQEKLIACGTGLTVVGMVLRFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+A+GLHGDVLRVAIIQAA+PQSITSFI+AKEYGLHAEVLSTAVIFG IVSLPVL
Sbjct: 301 AMAIGSIAVGLHGDVLRVAIIQAALPQSITSFIFAKEYGLHAEVLSTAVIFGMIVSLPVL 354

Query: 361 VAYYAALEFVH 371
           +AYYA LEFVH
Sbjct: 361 IAYYAVLEFVH 354

BLAST of MC08g2021 vs. TAIR 10
Match: AT5G16530.1 (Auxin efflux carrier family protein )

HSP 1 Score: 488.0 bits (1255), Expect = 6.6e-138
Identity = 257/371 (69.27%), Postives = 298/371 (80.32%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI   D YKV+ AM PLY AL LGYGSV+WW IF+ +QCDAIN+LVCYFTLPLFT +FT+
Sbjct: 1   MINCGDVYKVIEAMVPLYVALILGYGSVKWWHIFTRDQCDAINRLVCYFTLPLFTIEFTA 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
           H+DPF++N+ FIAAD +SK++IV VLA WA+ + KGSYCWSITSFSL TLTNSLVVGVPL
Sbjct: 61  HVDPFNMNYRFIAADVLSKVIIVTVLALWAKYSNKGSYCWSITSFSLCTLTNSLVVGVPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDHGKALQ 180
           AK MYGQ AVDLVVQ SV QAIVWLT+LLFVLE R+      AG +S++         + 
Sbjct: 121 AKAMYGQQAVDLVVQSSVFQAIVWLTLLLFVLEFRK------AGFSSNN---------IS 180

Query: 181 MVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMKKVWLKLAANPNSYACIIGFAWAFVAK 240
            V+    N+E        RET      SF  +M  VWLKLA NPN Y+CI+G AWAF++ 
Sbjct: 181 DVQVDNINIESGK-----RETVVVGEKSFLEVMSLVWLKLATNPNCYSCILGIAWAFISN 240

Query: 241 RWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMVLKFIAGPA 300
           RWH+E+P I+EGSILIMSKAG GTAMFNMGIFMALQEKLI CG SLT++GMVLKFIAGPA
Sbjct: 241 RWHLELPGILEGSILIMSKAGTGTAMFNMGIFMALQEKLIVCGTSLTVMGMVLKFIAGPA 300

Query: 301 AMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFGAIVSLPVL 360
           AMAIGS+ +GLHGDVLRVAIIQAA+PQSITSFI+AKEYGLHA+VLSTAVIFG +VSLPVL
Sbjct: 301 AMAIGSIVLGLHGDVLRVAIIQAALPQSITSFIFAKEYGLHADVLSTAVIFGMLVSLPVL 351

Query: 361 VAYYAALEFVH 372
           VAYYAALEF+H
Sbjct: 361 VAYYAALEFIH 351

BLAST of MC08g2021 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 259.2 bits (661), Expect = 5.0e-69
Identity = 185/610 (30.33%), Postives = 254/610 (41.64%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D Y V+ A+ PLY A+ L YGSV+WWKIFS +QC  IN+ V  F +PL +F F S
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
             DP+ +NF F+AAD + K++++V+LA WA  T  GS  W IT FSLSTL N+LV+G+PL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRL--------------------D 180
              MYG  A  L+VQ  V+Q I+W T+LLF+ E R  +L                    D
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 LVA--------------------------------------------------------- 240
           +V+                                                         
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 --------------GEASSSFGPGD----------------------------------- 300
                         G   S+FGP D                                   
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

Query: 361 ---------HGKALQMVEEGRKNL----------------------EGE----------- 368
                       A +  E+G K +                      EGE           
Sbjct: 361 SDVFGGGAGDNVATEQSEQGAKEIRMVVSDQPRKSGGDDIGGLDSGEGEREIEKATAGLN 420

BLAST of MC08g2021 vs. TAIR 10
Match: AT5G15100.1 (Auxin efflux carrier family protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.5e-69
Identity = 154/376 (40.96%), Postives = 219/376 (58.24%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D Y VV+A  PLY ++ LG+ S R  K+FS EQC  INK V  F++PL +F   S
Sbjct: 1   MISWLDIYHVVSATVPLYVSMTLGFLSARHLKLFSPEQCAGINKFVAKFSIPLLSFQIIS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLA----FWARCTPKGSYC-WSITSFSLSTLTNSLV 120
             +PF ++   I +D + K L+VVVLA    FW     +G    W IT  S+S L N+L+
Sbjct: 61  ENNPFKMSPKLILSDILQKFLVVVVLAMVLRFWHPTGGRGGKLGWVITGLSISVLPNTLI 120

Query: 121 VGVPLAKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRLDLVAGEASSSFGPGDH 180
           +G+P+   +YG  A  ++ Q  V+Q+++W TILLF+ EL   R  L +  AS      D 
Sbjct: 121 LGMPILSAIYGDEAASILEQIVVLQSLIWYTILLFLFELNAARA-LPSSGASLEHTGNDQ 180

Query: 181 GKALQMVEEGRKNLEGEAMEVEGRETSNSHRASFKPLMK---KVWLKLAANPNSYACIIG 240
            +A         N+E E  E E  E     R      MK   K W KL  NPN+YA +IG
Sbjct: 181 EEA---------NIEDEPKEEEDEEEVAIVRTRSVGTMKILLKAWRKLIINPNTYATLIG 240

Query: 241 FAWAFVAKRWHIEMPSIMEGSILIMSKAGIGTAMFNMGIFMALQEKLIACGASLTIIGMV 300
             WA +  R    +P +++ SI ++S  G+G AMF++G+FMA Q  +IACG  + II M+
Sbjct: 241 IIWATLHFRLGWNLPEMIDKSIHLLSDGGLGMAMFSLGLFMASQSSIIACGTKMAIITML 300

Query: 301 LKFIAGPAAMAIGSMAMGLHGDVLRVAIIQAAVPQSITSFIYAKEYGLHAEVLSTAVIFG 360
           LKF+ GPA M   +  + L   + +VAI+QAA+PQ +  F++AKEY LH E++ST VIFG
Sbjct: 301 LKFVLGPALMIASAYCIRLKSTLFKVAILQAALPQGVVPFVFAKEYNLHPEIISTGVIFG 360

Query: 361 AIVSLPVLVAYYAALE 369
            +++LP  +AYY  L+
Sbjct: 361 MLIALPTTLAYYFLLD 366

BLAST of MC08g2021 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.4e-68
Identity = 185/614 (30.13%), Postives = 254/614 (41.37%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D Y V+ A+ PLY A+ L YGSV+WWKIFS +QC  IN+ V  F +PL +F F S
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
             DP+ +NF F+AAD + K++++V+LA WA  T  GS  W IT FSLSTL N+LV+G+PL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRL--------------------D 180
              MYG  A  L+VQ  V+Q I+W T+LLF+ E R  +L                    D
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 LVA--------------------------------------------------------- 240
           +V+                                                         
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLMMTPRPSNLTGAEIYSLSSTPRGS 240

Query: 241 --------------GEASSSFGPGD----------------------------------- 300
                         G   S+FGP D                                   
Sbjct: 241 NFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENNAVKYGFYNNTNSSV 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 PAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSKASHDAKELHMFVWSSSASPV 360

Query: 361 ---------HGKALQMVEEGRKNL--------------------------EGE------- 368
                       A +  E+G K +                          EGE       
Sbjct: 361 SDVFGGGAGDNVATEQSEQGAKEIRMVVSDQPRKSNARGGGDDIGGLDSGEGEREIEKAT 420

BLAST of MC08g2021 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 245.4 bits (625), Expect = 7.4e-65
Identity = 185/613 (30.18%), Postives = 250/613 (40.78%), Query Frame = 0

Query: 1   MIGWEDAYKVVAAMAPLYFALALGYGSVRWWKIFSTEQCDAINKLVCYFTLPLFTFDFTS 60
           MI W D Y V+ A+ PLY A+ L YGSVRWWKIFS +QC  IN+ V  F +PL +F F S
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  HIDPFHLNFPFIAADAISKLLIVVVLAFWARCTPKGSYCWSITSFSLSTLTNSLVVGVPL 120
             +P+ +N  FIAAD + KL+++ +L  WA  T  GS  WSIT FSLSTL N+LV+G+PL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 AKIMYGQMAVDLVVQGSVVQAIVWLTILLFVLELRRTRL--------------------D 180
              MYG+ +  L+VQ  V+Q I+W T+LLF+ E R  ++                    D
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 LVA--------------------------------------------------------- 240
           +V+                                                         
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFYGGGGTNMTPRPSNLTGAEIYSLN 240

Query: 241 --------------------GEASSSFGPGD----------------------------- 300
                               G   S+FGP D                             
Sbjct: 241 TTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEESCAMASSPRF 300

Query: 301 ------------------------------------------HGKALQMV---------- 360
                                                       K L M           
Sbjct: 301 GYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELHMFVWGSNGSPVS 360

Query: 361 ------------EEGRKNLEGEAMEV---------------------------------- 368
                       E+  K+ +G A E+                                  
Sbjct: 361 DRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNAGPMNGDYGGEEESERVKEVPNGL 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFD09.2e-13769.27Auxin efflux carrier component 5 OS=Arabidopsis thaliana OX=3702 GN=PIN5 PE=2 SV... [more]
Q5JLM12.1e-11259.95Probable auxin efflux carrier component 5a OS=Oryza sativa subsp. japonica OX=39... [more]
D5A7J31.4e-10855.09Probable auxin efflux carrier component 5b OS=Oryza sativa subsp. japonica OX=39... [more]
Q6ZIB54.3e-10252.66Probable auxin efflux carrier component 5c OS=Oryza sativa subsp. japonica OX=39... [more]
Q9LFP69.1e-6840.96Auxin efflux carrier component 8 OS=Arabidopsis thaliana OX=3702 GN=PIN8 PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_022149923.11.44e-259100.00auxin efflux carrier component 5 [Momordica charantia][more]
XP_038889666.11.89e-22787.33auxin efflux carrier component 5 [Benincasa hispida][more]
XP_022937707.13.08e-22286.49auxin efflux carrier component 5 [Cucurbita moschata][more]
XP_004152830.18.81e-22286.79auxin efflux carrier component 5 [Cucumis sativus] >KAE8651707.1 hypothetical pr... [more]
XP_022966048.13.58e-22185.95auxin efflux carrier component 5 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1D8206.97e-260100.00Auxin efflux carrier component OS=Momordica charantia OX=3673 GN=LOC111018220 PE... [more]
A0A6J1FBZ51.49e-22286.49Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111444027 PE=... [more]
A0A6J1HQQ51.74e-22185.95Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465776 PE=3 ... [more]
A0A0A0LH238.89e-21986.25Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_2G074170 PE=3 S... [more]
A0A2C9VF502.15e-18373.85Auxin efflux carrier component OS=Manihot esculenta OX=3983 GN=MANES_08G104400 P... [more]
Match NameE-valueIdentityDescription
AT5G16530.16.6e-13869.27Auxin efflux carrier family protein [more]
AT2G01420.15.0e-6930.33Auxin efflux carrier family protein [more]
AT5G15100.16.5e-6940.96Auxin efflux carrier family protein [more]
AT2G01420.21.4e-6830.13Auxin efflux carrier family protein [more]
AT1G23080.37.4e-6530.18Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 179..199
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..370
NoneNo IPR availablePANTHERPTHR31752:SF2AUXIN EFFLUX CARRIER COMPONENT 5coord: 1..370
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 10..364
e-value: 3.2E-91
score: 305.6
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..367
e-value: 7.1E-89
score: 296.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g2021.1MC08g2021.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0080162 intracellular auxin transport
biological_process GO:0009555 pollen development
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity