MC08g1970 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1970 vs. ExPASy Swiss-Prot
Match: Q8W468 (Probable E3 ubiquitin-protein ligase ARI8 OS=Arabidopsis thaliana OX=3702 GN=ARI8 PE=2 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 2.4e-264
Identity = 435/576 (75.52%), Postives = 495/576 (85.94%), Query Frame = 0

Query: 21  EDAFYSGGDD--DAAAIDSDDADVGDYEFIDNDSDDSDDMVSYRHQQNYIILAEADIRQR 80
           +D FYSG ++  D A  D DDAD G+YEF+D+ +DDSDD++  R QQNY +L+EADI + 
Sbjct: 4   DDDFYSGTENYSDYADSDEDDAD-GEYEFVDDAADDSDDLIFRRRQQNYSVLSEADICKL 63

Query: 81  QEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEEKVRRAVGLLEKPVLQSSN 140
           QE+DI R+STVLSIS+ +++ILLR+YNW VS+VHDEWFADEEKVR AVGLLEKPV+    
Sbjct: 64  QEDDISRISTVLSISRNSSAILLRHYNWCVSRVHDEWFADEEKVRDAVGLLEKPVVDFPT 123

Query: 141 ELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSINDGPGCLMLRCPDPSCGAAV 200
           + EL CGICFE +  DK+ +AACGHPFC++CW GYI+T+INDGPGCL LRCPDPSC AAV
Sbjct: 124 DGELDCGICFETFLSDKLHAAACGHPFCDSCWEGYITTAINDGPGCLTLRCPDPSCRAAV 183

Query: 201 GQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDYAVDFIVGSGSYDVTCRCS 260
           GQDMINLLA D DK+KY  YFVRSYVEDNRKTKWCPAPGCDYAV+F+VGSG+YDV CRC 
Sbjct: 184 GQDMINLLAPDKDKQKYTSYFVRSYVEDNRKTKWCPAPGCDYAVNFVVGSGNYDVNCRCC 243

Query: 261 YSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI 320
           YSFCWNC EEAHRPVDC TV+KW+LKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI
Sbjct: 244 YSFCWNCAEEAHRPVDCDTVSKWVLKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI 303

Query: 321 TCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGVYDDAEKRREMAKNSLERY 380
           TCTPPCKFEFCWLCLG W++HGE+TGGFYACNRYE AKQ+G+YD+ EKRREMAKNSLERY
Sbjct: 304 TCTPPCKFEFCWLCLGAWTEHGEKTGGFYACNRYEAAKQDGIYDETEKRREMAKNSLERY 363

Query: 381 THYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQLKFISEAWLQIVECRRVL 440
           THYYERWATNQSSRQKAL DL +MQT  +EKLSDIQCQPESQLKFI EAWLQIVECRRVL
Sbjct: 364 THYYERWATNQSSRQKALLDLKKMQTDDIEKLSDIQCQPESQLKFIIEAWLQIVECRRVL 423

Query: 441 KWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKELQVYLNADGPSKDFNEFR 500
           KWTYAYG+Y+P++EH KR FFEYLQGEAESGLERLHQCAEKEL  YL+A GPS+DFNEFR
Sbjct: 424 KWTYAYGFYIPDQEHGKRVFFEYLQGEAESGLERLHQCAEKELLPYLDAKGPSEDFNEFR 483

Query: 501 TKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKSTGSCRSKGGKGKMSAFRA 560
           TKLAGLTSVT+NYFENLVRALENGL+DV+SH    RT+SSKS       GGK K S+ +A
Sbjct: 484 TKLAGLTSVTKNYFENLVRALENGLSDVNSHDAYDRTSSSKSL------GGKTKGSSSKA 543

Query: 561 SGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 595
           S S     D+ HW CE+CT+ N +S TICQMC+  R
Sbjct: 544 SSS-----DSSHWPCEYCTYVNPRSTTICQMCEHGR 567

BLAST of MC08g1970 vs. ExPASy Swiss-Prot
Match: Q84RR0 (Probable E3 ubiquitin-protein ligase ARI7 OS=Arabidopsis thaliana OX=3702 GN=ARI7 PE=2 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 1.3e-249
Identity = 420/568 (73.94%), Postives = 483/568 (85.04%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           M+SE+  DM DAH  D E  ED FYSGG DD    DSDD +  DY F++ D+DDS  + S
Sbjct: 1   MDSEE--DMLDAH--DMESGEDDFYSGGTDDCN--DSDDGE-PDYGFVEEDADDSAMIAS 60

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           +R Q+N+ +L E DIR+ Q ++I RVS VLSI++V ASILLR+++WSV +VHDEWFADEE
Sbjct: 61  HRSQKNFCVLREEDIRRHQMDNIERVSVVLSITEVEASILLRHFHWSVGRVHDEWFADEE 120

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           +VR+ VG+LE  V+  S++ EL CGICF+ Y  +KI S +CGHPFC TCWTGYIST+IND
Sbjct: 121 RVRKTVGILESHVVPPSDDSELTCGICFDSYPPEKIASVSCGHPFCTTCWTGYISTTIND 180

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLMLRCPDPSC AAVG DM++ LAS+D+K+KY RYF+RSY+EDNRK KWCPAPGCD+
Sbjct: 181 GPGCLMLRCPDPSCLAAVGHDMVDKLASEDEKEKYNRYFLRSYIEDNRKMKWCPAPGCDF 240

Query: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300
           A+DF+ GSG+YDV+C CS+SFCWNCTEEAHRPVDC TV+KWILKNSAESENMNWILANSK
Sbjct: 241 AIDFVAGSGNYDVSCLCSFSFCWNCTEEAHRPVDCSTVSKWILKNSAESENMNWILANSK 300

Query: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360
           PCP+CKRPIEKNQGCMH+TCTPPCK+EFCWLCLG W DHGERTGGFYACNRYE AKQEG 
Sbjct: 301 PCPRCKRPIEKNQGCMHMTCTPPCKYEFCWLCLGAWMDHGERTGGFYACNRYEVAKQEGQ 360

Query: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420
           YD+ E+RREMAKNSLERYTHYYERWA+NQ+SRQKA+ADL Q Q  +LEKLSD QC PESQ
Sbjct: 361 YDETERRREMAKNSLERYTHYYERWASNQTSRQKAMADLQQAQMQNLEKLSDKQCTPESQ 420

Query: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480
           LKFI EAWLQI+ECRRVLKWTYAYGYYLPE EHAKRQFFEYLQGEAESGLERLHQC EK+
Sbjct: 421 LKFILEAWLQIIECRRVLKWTYAYGYYLPEHEHAKRQFFEYLQGEAESGLERLHQCVEKD 480

Query: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTC-SRTASSK 540
           L  +L A+GPSKDFN+FRTKLAGLTSVT+NYFENLV+ALENGLADVDSH  C S++ SSK
Sbjct: 481 LVQFLIAEGPSKDFNDFRTKLAGLTSVTKNYFENLVKALENGLADVDSHAACSSKSTSSK 540

Query: 541 STGSCRSKGGKGKMSAFRASGSSRNIDD 568
           STG      GKGK S+ R  GSSRN DD
Sbjct: 541 STGCSSKTRGKGKGSS-RTGGSSRNPDD 560

BLAST of MC08g1970 vs. ExPASy Swiss-Prot
Match: Q8L829 (Probable E3 ubiquitin-protein ligase ARI5 OS=Arabidopsis thaliana OX=3702 GN=ARI5 PE=2 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 5.1e-230
Identity = 386/566 (68.20%), Postives = 466/566 (82.33%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           M+S+D     D HD D  D +  +YSGG       D +D+D  D+ F + D+DD+  + S
Sbjct: 1   MDSDD-----DMHDMDSVDYD--YYSGG-----TYDDNDSDETDFGFGEADTDDAAIIAS 60

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           YR + NY++L E DIR+ Q +D+ RVS VLSI+ V AS LL +Y+WSVSKV+DEWFADEE
Sbjct: 61  YRSKSNYVVLKEEDIRRHQNDDVGRVSAVLSITDVEASTLLLHYHWSVSKVNDEWFADEE 120

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           +VRR VG+LE PV+ + +  E  CGICF+ Y+ ++I S +CGHPFC TCWTGYIST+IND
Sbjct: 121 RVRRTVGILEGPVVTTPDGREFTCGICFDSYTLEEIVSVSCGHPFCATCWTGYISTTIND 180

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLML+CPDPSC AA+G+DMI+ LAS +DK+KY+RYF+RSYVE NR+ KWCPAPGC++
Sbjct: 181 GPGCLMLKCPDPSCPAAIGRDMIDKLASKEDKEKYYRYFLRSYVEVNREMKWCPAPGCEH 240

Query: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300
           A+DF  G+ SYDV+C CS+SFCWNCTEEAHRPVDC TV KWILKNSAESENMNWILANSK
Sbjct: 241 AIDFAGGTESYDVSCLCSHSFCWNCTEEAHRPVDCDTVGKWILKNSAESENMNWILANSK 300

Query: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360
           PCPKCKRPIEKN GCMH+TCTPPCKFEFCWLCL  W++HGERTGGFYACNRYE AKQEG+
Sbjct: 301 PCPKCKRPIEKNHGCMHMTCTPPCKFEFCWLCLNAWTEHGERTGGFYACNRYEAAKQEGL 360

Query: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420
           YD+AE+RREMAKNSLERYTHYYERWA+NQ SRQKA+ DL +MQ+  L KLSDIQC PESQ
Sbjct: 361 YDEAERRREMAKNSLERYTHYYERWASNQVSRQKAMGDLQKMQSEKLGKLSDIQCTPESQ 420

Query: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480
           LKFI+EAWLQI+ECRRVLKWTYAYGYYL  ++HAK+ FFEYLQGEAESGLERLH+C EK+
Sbjct: 421 LKFIAEAWLQIIECRRVLKWTYAYGYYL--QDHAKKPFFEYLQGEAESGLERLHKCVEKD 480

Query: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS 540
           ++V+  A+GPS++FN FRTKL GLTS+T+ +FENLV+ALENGLADVDS    S+ A+SK 
Sbjct: 481 IEVFELAEGPSEEFNHFRTKLTGLTSITKTFFENLVKALENGLADVDSQAASSKPANSKP 540

Query: 541 TGSCRSKGGKGKMSAFRASGSSRNID 567
           +   +  GGKGK S+ +  GSSRN D
Sbjct: 541 SSKTKG-GGKGKGSS-KNGGSSRNPD 550

BLAST of MC08g1970 vs. ExPASy Swiss-Prot
Match: P0C8K8 (Putative E3 ubiquitin-protein ligase ARI6 OS=Arabidopsis thaliana OX=3702 GN=ARI6 PE=5 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 9.4e-216
Identity = 365/563 (64.83%), Postives = 452/563 (80.28%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           M+S+D  DM DAHD D  D +  F SGG D     D +D D  DY F + D+DD+  +  
Sbjct: 2   MDSDD--DMLDAHDMDSVDYD--FDSGGTD-----DDNDIDETDYVFGEADTDDAAIIAY 61

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           +R Q NY++L E DIR+ Q++D+ RVS VLSI+ V AS+LL +Y+WSVSKV+DEWFADE+
Sbjct: 62  HRSQINYVVLKEEDIRRHQKDDVGRVSVVLSITDVQASLLLLHYHWSVSKVNDEWFADED 121

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           +VRR VG+LE P   + +  E  CGICFE Y  ++  S +CGHPFC TCWTGYISTSIND
Sbjct: 122 RVRRTVGILEGP---APDGREFTCGICFESYPLEETISVSCGHPFCATCWTGYISTSIND 181

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLML+CP P C AA+G+DMI+ L S +DK++Y+RYF+RSYVE NR+ K CPAPGC++
Sbjct: 182 GPGCLMLKCPYPCCPAAIGRDMIDNLCSKEDKERYYRYFLRSYVEVNREMKCCPAPGCEH 241

Query: 241 AVDFIVGSGS-YDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANS 300
           A+ F  G+ S YDV+C CS+SFCWNC+EEAHRPVDC TV KWILKNS ESENMNWILANS
Sbjct: 242 AISFAAGTESNYDVSCLCSHSFCWNCSEEAHRPVDCDTVGKWILKNSTESENMNWILANS 301

Query: 301 KPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEG 360
           KPCPKCKRPIEKN GCMH+TCTPPCKFEFCWLCL  W++HGE +GG+YACNRYE AK++G
Sbjct: 302 KPCPKCKRPIEKNHGCMHMTCTPPCKFEFCWLCLNAWTEHGESSGGYYACNRYEAAKKQG 361

Query: 361 VYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPES 420
           +YD+AE+RREMAKNSLE+YTHYY+RWA+NQ SRQKA+ DL +MQ+  L KLSDIQC  ES
Sbjct: 362 LYDEAERRREMAKNSLEKYTHYYKRWASNQVSRQKAMGDLQKMQSEKLRKLSDIQCTSES 421

Query: 421 QLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEK 480
           QLKFI+EAWLQI+ECRRVLKWTYAYGYY+P+ +H K+QFFEYLQGEAESGLERLH+C E 
Sbjct: 422 QLKFIAEAWLQIIECRRVLKWTYAYGYYVPD-DHTKKQFFEYLQGEAESGLERLHECIEN 481

Query: 481 ELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSK 540
           +++V+   +GPS++FN FRTKL  LTS+T+ +F+NLV+ALENGLADVDSH   S+ A+ K
Sbjct: 482 DIEVFEFGEGPSEEFNHFRTKLTDLTSITKTFFQNLVKALENGLADVDSHAASSKPANCK 541

Query: 541 STGSCRSKGGKGKMSAFRASGSS 563
            + + +  GGKGK  A   +GS+
Sbjct: 542 PSSNTKD-GGKGKKEALTMAGSA 550

BLAST of MC08g1970 vs. ExPASy Swiss-Prot
Match: Q9SKC2 (Probable E3 ubiquitin-protein ligase ARI11 OS=Arabidopsis thaliana OX=3702 GN=ARI11 PE=2 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.5e-168
Identity = 286/520 (55.00%), Postives = 379/520 (72.88%), Query Frame = 0

Query: 16  DDEDNEDAFYSGGDDDAAAIDSDDADV-GDYEFIDNDSDDSDDMVSYRHQQNYIILAEAD 75
           D E  E+  YS G +D   I+S + D+  D   + +D +  DD +S R +++Y+++ E D
Sbjct: 10  DIESGEEDLYSDGGNDIIDIESGEEDLYSDGGNVSDDYNPVDDTIS-RSEKSYVVVKEED 69

Query: 76  IRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEEKVRRAVGLLEKPVL 135
           I + Q +DI +VSTVLS+S+V + +LL +Y+W VSK+ DEWF DEE++R+ VG+L++PV+
Sbjct: 70  ILKLQRDDIEQVSTVLSVSQVESIVLLLHYHWCVSKLEDEWFTDEERIRKTVGILKEPVV 129

Query: 136 Q-SSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSINDGPGCLMLRCPDPS 195
             +  E+++ CGICFE Y+R +I   +CGHP+C TCWTGYI+T I DGPGCL ++CP+PS
Sbjct: 130 DVNGTEVDIQCGICFESYTRKEIARVSCGHPYCKTCWTGYITTKIEDGPGCLRVKCPEPS 189

Query: 196 CGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDYAVDF-IVGSGSYD 255
           C A VGQDMI+ +    DK KY+RYF+RSYVED +K KWCP+PGC+YAV+F + GS SYD
Sbjct: 190 CYAVVGQDMIDEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGCEYAVEFGVNGSSSYD 249

Query: 256 VTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSKPCPKCKRPIEKN 315
           V+C CSY FCWNC E+AH PVDC TV+KW+LKN  ESENMNWILA +KPCPKCKRPIEKN
Sbjct: 250 VSCLCSYKFCWNCCEDAHSPVDCETVSKWLLKNKDESENMNWILAKTKPCPKCKRPIEKN 309

Query: 316 QGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGVYDDAEKRREMAK 375
            GC H++C+ PC+  FCW CL P SDH        ACN ++   ++      E +R+ AK
Sbjct: 310 TGCNHMSCSAPCRHYFCWACLQPLSDH-------KACNAFKADNED------ETKRKRAK 369

Query: 376 NSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQLKFISEAWLQIV 435
           ++++RYTH+YERWA NQSSR KA++DL + Q+V L++LSDIQ  PE+QL F  +AWLQI+
Sbjct: 370 DAIDRYTHFYERWAFNQSSRLKAMSDLEKWQSVELKQLSDIQSTPETQLSFTVDAWLQII 429

Query: 436 ECRRVLKWTYAYGYYLPEREHAKRQF-----FEYLQGEAESGLERLHQCAEKELQVYL-N 495
           ECRRVLKWTYAYGYY+  +E  KR F           EAE+GLERLH CAE+EL+ ++  
Sbjct: 430 ECRRVLKWTYAYGYYILSQERNKRVFARTFSLSCCSAEAENGLERLHHCAEEELKQFIGK 489

Query: 496 ADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADV 527
            + PSK+F E R KL  LT  T+ YFENLV+ALENGL DV
Sbjct: 490 IEDPSKNFGELRAKLIDLTKATKTYFENLVKALENGLVDV 515

BLAST of MC08g1970 vs. NCBI nr
Match: XP_022144490.1 (probable E3 ubiquitin-protein ligase ARI8 [Momordica charantia])

HSP 1 Score: 1221 bits (3160), Expect = 0.0
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS
Sbjct: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE
Sbjct: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND
Sbjct: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY
Sbjct: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240

Query: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300
           AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK
Sbjct: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300

Query: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360
           PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV
Sbjct: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360

Query: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420
           YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ
Sbjct: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420

Query: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480
           LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE
Sbjct: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480

Query: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS 540
           LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS
Sbjct: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS 540

Query: 541 TGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           TGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR
Sbjct: 541 TGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594

BLAST of MC08g1970 vs. NCBI nr
Match: XP_022965653.1 (probable E3 ubiquitin-protein ligase ARI8 [Cucurbita maxima])

HSP 1 Score: 1165 bits (3015), Expect = 0.0
Identity = 568/597 (95.14%), Postives = 577/597 (96.65%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDED---NEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDD 60
           MESEDELDMHDAHDDDD+D   NE+ FYSGG+DDAAAIDSDDADVGDYEF+DNDSDDSDD
Sbjct: 1   MESEDELDMHDAHDDDDDDSVDNEEDFYSGGEDDAAAIDSDDADVGDYEFVDNDSDDSDD 60

Query: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120
           MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA
Sbjct: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120

Query: 121 DEEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTS 180
           DEEKVRRAVG+LEKPVL  SN LELPCGICFEIYSRD IQSAACGHPFCNTCWTGYI TS
Sbjct: 121 DEEKVRRAVGILEKPVLPHSNALELPCGICFEIYSRDNIQSAACGHPFCNTCWTGYIRTS 180

Query: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPG 240
           INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKY RYFVRSYVEDNRKTKWCPAPG
Sbjct: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYSRYFVRSYVEDNRKTKWCPAPG 240

Query: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300
           CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA
Sbjct: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300

Query: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360
           NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ
Sbjct: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360

Query: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQP 420
           EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSD QCQP
Sbjct: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDTQCQP 420

Query: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCA 480
           ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKR FFEYLQGEAESGLERLHQCA
Sbjct: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRLFFEYLQGEAESGLERLHQCA 480

Query: 481 EKELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTAS 540
           EKELQ Y+ ADGPS++FNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDS GTCSRTAS
Sbjct: 481 EKELQAYITADGPSREFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSQGTCSRTAS 540

Query: 541 SKSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           SKS G  RSKGGKGKMSAFRASGSSR IDDTDHWSCEHCTFANVKS TICQMCQQRR
Sbjct: 541 SKSIGGGRSKGGKGKMSAFRASGSSRTIDDTDHWSCEHCTFANVKSFTICQMCQQRR 597

BLAST of MC08g1970 vs. NCBI nr
Match: KAG6586185.1 (putative E3 ubiquitin-protein ligase ARI8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1165 bits (3015), Expect = 0.0
Identity = 568/597 (95.14%), Postives = 577/597 (96.65%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDED---NEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDD 60
           MESEDELDMHDAHDDDD+D   NE+ FYSGG+DDAAAIDSDDADVGDYEF+DNDSDDSDD
Sbjct: 1   MESEDELDMHDAHDDDDDDSVDNEEDFYSGGEDDAAAIDSDDADVGDYEFVDNDSDDSDD 60

Query: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120
           MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA
Sbjct: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120

Query: 121 DEEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTS 180
           DEEKVRRAVG+LEKPVL  SN LELPCGICFEIYSRD IQSAACGHPFCNTCWTGYI TS
Sbjct: 121 DEEKVRRAVGILEKPVLPPSNALELPCGICFEIYSRDNIQSAACGHPFCNTCWTGYIRTS 180

Query: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPG 240
           INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKY RYFVRSYVEDNRKTKWCPAPG
Sbjct: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYSRYFVRSYVEDNRKTKWCPAPG 240

Query: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300
           CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA
Sbjct: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300

Query: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360
           NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ
Sbjct: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360

Query: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQP 420
           EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSD QCQP
Sbjct: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDTQCQP 420

Query: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCA 480
           ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKR FFEYLQGEAESGLERLHQCA
Sbjct: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRLFFEYLQGEAESGLERLHQCA 480

Query: 481 EKELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTAS 540
           EKELQ Y+ ADGPS++FNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDS GTCSRTAS
Sbjct: 481 EKELQAYITADGPSREFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSQGTCSRTAS 540

Query: 541 SKSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           SKS G  RSKGGKGKMSAFRASGSSR IDDTDHWSCEHCTFANVKS TICQMCQQRR
Sbjct: 541 SKSIGGGRSKGGKGKMSAFRASGSSRTIDDTDHWSCEHCTFANVKSFTICQMCQQRR 597

BLAST of MC08g1970 vs. NCBI nr
Match: XP_022938050.1 (probable E3 ubiquitin-protein ligase ARI8 [Cucurbita moschata])

HSP 1 Score: 1165 bits (3013), Expect = 0.0
Identity = 567/596 (95.13%), Postives = 577/596 (96.81%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDE--DNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDM 60
           MESEDELDMHDAHDDDD+  DNE+ FYSGG+DDAAAIDSDDADVGDYEF+DNDSDDSDDM
Sbjct: 1   MESEDELDMHDAHDDDDDSVDNEEDFYSGGEDDAAAIDSDDADVGDYEFVDNDSDDSDDM 60

Query: 61  VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD 120
           VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLR+YNWSVSKVHDEWFAD
Sbjct: 61  VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRHYNWSVSKVHDEWFAD 120

Query: 121 EEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSI 180
           EEKVRRAVG+LEKPVL  SN LELPCGICFEIYSRD IQSAACGHPFCNTCWTGYI TSI
Sbjct: 121 EEKVRRAVGILEKPVLPPSNALELPCGICFEIYSRDNIQSAACGHPFCNTCWTGYIRTSI 180

Query: 181 NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGC 240
           NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKY RYFVRSYVEDNRKTKWCPAPGC
Sbjct: 181 NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYSRYFVRSYVEDNRKTKWCPAPGC 240

Query: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN 300
           DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN
Sbjct: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN 300

Query: 301 SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360
           SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE
Sbjct: 301 SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360

Query: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPE 420
           GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSD QCQPE
Sbjct: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDTQCQPE 420

Query: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE 480
           SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKR FFEYLQGEAESGLERLHQCAE
Sbjct: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRLFFEYLQGEAESGLERLHQCAE 480

Query: 481 KELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASS 540
           KELQ Y+ ADGPS++FNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDS GTCSRTASS
Sbjct: 481 KELQAYITADGPSREFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSQGTCSRTASS 540

Query: 541 KSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           KS G  RSKGGKGKMSAFRASGSSR IDDTDHWSCEHCTFANVKS TICQMCQQRR
Sbjct: 541 KSIGGSRSKGGKGKMSAFRASGSSRTIDDTDHWSCEHCTFANVKSFTICQMCQQRR 596

BLAST of MC08g1970 vs. NCBI nr
Match: XP_023537577.1 (probable E3 ubiquitin-protein ligase ARI8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1164 bits (3011), Expect = 0.0
Identity = 567/597 (94.97%), Postives = 576/597 (96.48%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDED---NEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDD 60
           MESEDELDMHDAHDDDD+D   NE+ FYSGG+DDAAAIDSDDADVGDYEF+DNDSDDSDD
Sbjct: 1   MESEDELDMHDAHDDDDDDSVDNEEDFYSGGEDDAAAIDSDDADVGDYEFVDNDSDDSDD 60

Query: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120
           MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA
Sbjct: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120

Query: 121 DEEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTS 180
           DEEKVRRAVG+LEKPVL  SN LELPCGICFEIYSRD IQSAACGHPFCNTCWTGYI TS
Sbjct: 121 DEEKVRRAVGILEKPVLPHSNALELPCGICFEIYSRDNIQSAACGHPFCNTCWTGYIRTS 180

Query: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPG 240
           INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKY RYFVRSYVEDNRKTKWCPAPG
Sbjct: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYSRYFVRSYVEDNRKTKWCPAPG 240

Query: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300
           CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA
Sbjct: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300

Query: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360
           NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ
Sbjct: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360

Query: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQP 420
           EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSD QCQP
Sbjct: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDTQCQP 420

Query: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCA 480
           ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKR FFEYLQGEAESGLERLHQCA
Sbjct: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRLFFEYLQGEAESGLERLHQCA 480

Query: 481 EKELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTAS 540
           EKELQ Y+ ADGPS++FNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDS GTCSRTAS
Sbjct: 481 EKELQAYITADGPSREFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSQGTCSRTAS 540

Query: 541 SKSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           SKS G  RSKGGKGKMSAFR SGSSR IDDTDHWSCEHCTFANVKS TICQMCQQRR
Sbjct: 541 SKSIGGGRSKGGKGKMSAFRGSGSSRTIDDTDHWSCEHCTFANVKSFTICQMCQQRR 597

BLAST of MC08g1970 vs. ExPASy TrEMBL
Match: A0A6J1CRR9 (RBR-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111014168 PE=3 SV=1)

HSP 1 Score: 1221 bits (3160), Expect = 0.0
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS
Sbjct: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE
Sbjct: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND
Sbjct: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY
Sbjct: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240

Query: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300
           AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK
Sbjct: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300

Query: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360
           PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV
Sbjct: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360

Query: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420
           YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ
Sbjct: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420

Query: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480
           LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE
Sbjct: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480

Query: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS 540
           LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS
Sbjct: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS 540

Query: 541 TGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           TGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR
Sbjct: 541 TGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594

BLAST of MC08g1970 vs. ExPASy TrEMBL
Match: A0A6J1HPL6 (RBR-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465486 PE=3 SV=1)

HSP 1 Score: 1165 bits (3015), Expect = 0.0
Identity = 568/597 (95.14%), Postives = 577/597 (96.65%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDED---NEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDD 60
           MESEDELDMHDAHDDDD+D   NE+ FYSGG+DDAAAIDSDDADVGDYEF+DNDSDDSDD
Sbjct: 1   MESEDELDMHDAHDDDDDDSVDNEEDFYSGGEDDAAAIDSDDADVGDYEFVDNDSDDSDD 60

Query: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120
           MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA
Sbjct: 61  MVSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFA 120

Query: 121 DEEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTS 180
           DEEKVRRAVG+LEKPVL  SN LELPCGICFEIYSRD IQSAACGHPFCNTCWTGYI TS
Sbjct: 121 DEEKVRRAVGILEKPVLPHSNALELPCGICFEIYSRDNIQSAACGHPFCNTCWTGYIRTS 180

Query: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPG 240
           INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKY RYFVRSYVEDNRKTKWCPAPG
Sbjct: 181 INDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYSRYFVRSYVEDNRKTKWCPAPG 240

Query: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300
           CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA
Sbjct: 241 CDYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILA 300

Query: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360
           NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ
Sbjct: 301 NSKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQ 360

Query: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQP 420
           EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSD QCQP
Sbjct: 361 EGVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDTQCQP 420

Query: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCA 480
           ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKR FFEYLQGEAESGLERLHQCA
Sbjct: 421 ESQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRLFFEYLQGEAESGLERLHQCA 480

Query: 481 EKELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTAS 540
           EKELQ Y+ ADGPS++FNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDS GTCSRTAS
Sbjct: 481 EKELQAYITADGPSREFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSQGTCSRTAS 540

Query: 541 SKSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           SKS G  RSKGGKGKMSAFRASGSSR IDDTDHWSCEHCTFANVKS TICQMCQQRR
Sbjct: 541 SKSIGGGRSKGGKGKMSAFRASGSSRTIDDTDHWSCEHCTFANVKSFTICQMCQQRR 597

BLAST of MC08g1970 vs. ExPASy TrEMBL
Match: A0A6J1FIK3 (RBR-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444256 PE=3 SV=1)

HSP 1 Score: 1165 bits (3013), Expect = 0.0
Identity = 567/596 (95.13%), Postives = 577/596 (96.81%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDE--DNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDM 60
           MESEDELDMHDAHDDDD+  DNE+ FYSGG+DDAAAIDSDDADVGDYEF+DNDSDDSDDM
Sbjct: 1   MESEDELDMHDAHDDDDDSVDNEEDFYSGGEDDAAAIDSDDADVGDYEFVDNDSDDSDDM 60

Query: 61  VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD 120
           VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLR+YNWSVSKVHDEWFAD
Sbjct: 61  VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRHYNWSVSKVHDEWFAD 120

Query: 121 EEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSI 180
           EEKVRRAVG+LEKPVL  SN LELPCGICFEIYSRD IQSAACGHPFCNTCWTGYI TSI
Sbjct: 121 EEKVRRAVGILEKPVLPPSNALELPCGICFEIYSRDNIQSAACGHPFCNTCWTGYIRTSI 180

Query: 181 NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGC 240
           NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKY RYFVRSYVEDNRKTKWCPAPGC
Sbjct: 181 NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYSRYFVRSYVEDNRKTKWCPAPGC 240

Query: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN 300
           DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN
Sbjct: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN 300

Query: 301 SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360
           SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE
Sbjct: 301 SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360

Query: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPE 420
           GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSD QCQPE
Sbjct: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDTQCQPE 420

Query: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE 480
           SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKR FFEYLQGEAESGLERLHQCAE
Sbjct: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRLFFEYLQGEAESGLERLHQCAE 480

Query: 481 KELQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASS 540
           KELQ Y+ ADGPS++FNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDS GTCSRTASS
Sbjct: 481 KELQAYITADGPSREFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSQGTCSRTASS 540

Query: 541 KSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           KS G  RSKGGKGKMSAFRASGSSR IDDTDHWSCEHCTFANVKS TICQMCQQRR
Sbjct: 541 KSIGGSRSKGGKGKMSAFRASGSSRTIDDTDHWSCEHCTFANVKSFTICQMCQQRR 596

BLAST of MC08g1970 vs. ExPASy TrEMBL
Match: A0A1S3B4U8 (RBR-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485827 PE=3 SV=1)

HSP 1 Score: 1161 bits (3004), Expect = 0.0
Identity = 563/597 (94.30%), Postives = 578/597 (96.82%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDE--DNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDM 60
           MESEDELDMHDAHDDDD+  DNE+ FYSGGDDDAA IDSDDADVGDYEF+DNDSDDSDDM
Sbjct: 1   MESEDELDMHDAHDDDDDSVDNEEDFYSGGDDDAAGIDSDDADVGDYEFVDNDSDDSDDM 60

Query: 61  VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD 120
           VSYRHQQNYIILAEADI+Q QEEDI RVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD
Sbjct: 61  VSYRHQQNYIILAEADIQQCQEEDITRVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD 120

Query: 121 EEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSI 180
           EEKVRR+VGLL+KPVL+ SNELELPCGICFEIY  DKIQSAACGHPFCN CWTGYISTSI
Sbjct: 121 EEKVRRSVGLLQKPVLRHSNELELPCGICFEIYPLDKIQSAACGHPFCNACWTGYISTSI 180

Query: 181 NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGC 240
           NDGPGCLMLRCPDPSCGAA+ QDMINLL S+DDKKKYFRYFVRSYVEDNRKTKWCPAPGC
Sbjct: 181 NDGPGCLMLRCPDPSCGAAIDQDMINLLVSEDDKKKYFRYFVRSYVEDNRKTKWCPAPGC 240

Query: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN 300
           DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDC TVA+WILKNSAESENMNWILAN
Sbjct: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCVTVARWILKNSAESENMNWILAN 300

Query: 301 SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360
           SKPCPKCKRPIEKNQGCMH+TCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE
Sbjct: 301 SKPCPKCKRPIEKNQGCMHLTCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360

Query: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPE 420
           GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQP+
Sbjct: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPQ 420

Query: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE 480
           SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE
Sbjct: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE 480

Query: 481 KELQVYLNA-DGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTAS 540
           KEL  YLNA DGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGL+DVDSHGTCS+TAS
Sbjct: 481 KELHAYLNAADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLSDVDSHGTCSKTAS 540

Query: 541 SKSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           SKSTG CRSKGGKGKMSAFR SGSSRNIDDTDHWSCEHCTFAN+KSAT CQMCQQRR
Sbjct: 541 SKSTGGCRSKGGKGKMSAFRGSGSSRNIDDTDHWSCEHCTFANLKSATFCQMCQQRR 597

BLAST of MC08g1970 vs. ExPASy TrEMBL
Match: A0A1S3B4Y9 (RBR-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485827 PE=3 SV=1)

HSP 1 Score: 1094 bits (2830), Expect = 0.0
Identity = 537/597 (89.95%), Postives = 551/597 (92.29%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDE--DNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDM 60
           MESEDELDMHDAHDDDD+  DNE+ FYSGGDDDAA IDSDDADVGDYEF+DNDSDDSDDM
Sbjct: 1   MESEDELDMHDAHDDDDDSVDNEEDFYSGGDDDAAGIDSDDADVGDYEFVDNDSDDSDDM 60

Query: 61  VSYRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD 120
           VSYRHQQNYIILAEADI+Q QEEDI RVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD
Sbjct: 61  VSYRHQQNYIILAEADIQQCQEEDITRVSTVLSISKVAASILLRYYNWSVSKVHDEWFAD 120

Query: 121 EEKVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSI 180
           EEKVRR+VGLL+KPVL+ SNELELPCGICFEIY  DKIQSAACGHPFCN CWTGYISTSI
Sbjct: 121 EEKVRRSVGLLQKPVLRHSNELELPCGICFEIYPLDKIQSAACGHPFCNACWTGYISTSI 180

Query: 181 NDGPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGC 240
           NDGPGCLMLRCPDPSCGAA+ QDMINLL S+DDKKKYFRYFVRSYVEDNRKTKWCPAPGC
Sbjct: 181 NDGPGCLMLRCPDPSCGAAIDQDMINLLVSEDDKKKYFRYFVRSYVEDNRKTKWCPAPGC 240

Query: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILAN 300
           DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDC TVA+WILKNSAESENMNWILAN
Sbjct: 241 DYAVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCVTVARWILKNSAESENMNWILAN 300

Query: 301 SKPCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360
           SKPCPKCKRPIEKNQGCMH+TCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE
Sbjct: 301 SKPCPKCKRPIEKNQGCMHLTCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQE 360

Query: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPE 420
           GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQP+
Sbjct: 361 GVYDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPQ 420

Query: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE 480
           SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE
Sbjct: 421 SQLKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAE 480

Query: 481 KELQVYLNA-DGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTAS 540
           KEL  YLNA DGPSKDFNEFRTKLAGLTS                           +TAS
Sbjct: 481 KELHAYLNAADGPSKDFNEFRTKLAGLTS---------------------------KTAS 540

Query: 541 SKSTGSCRSKGGKGKMSAFRASGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 594
           SKSTG CRSKGGKGKMSAFR SGSSRNIDDTDHWSCEHCTFAN+KSAT CQMCQQRR
Sbjct: 541 SKSTGGCRSKGGKGKMSAFRGSGSSRNIDDTDHWSCEHCTFANLKSATFCQMCQQRR 570

BLAST of MC08g1970 vs. TAIR 10
Match: AT1G65430.1 (IBR domain-containing protein )

HSP 1 Score: 912.5 bits (2357), Expect = 1.7e-265
Identity = 435/576 (75.52%), Postives = 495/576 (85.94%), Query Frame = 0

Query: 21  EDAFYSGGDD--DAAAIDSDDADVGDYEFIDNDSDDSDDMVSYRHQQNYIILAEADIRQR 80
           +D FYSG ++  D A  D DDAD G+YEF+D+ +DDSDD++  R QQNY +L+EADI + 
Sbjct: 4   DDDFYSGTENYSDYADSDEDDAD-GEYEFVDDAADDSDDLIFRRRQQNYSVLSEADICKL 63

Query: 81  QEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEEKVRRAVGLLEKPVLQSSN 140
           QE+DI R+STVLSIS+ +++ILLR+YNW VS+VHDEWFADEEKVR AVGLLEKPV+    
Sbjct: 64  QEDDISRISTVLSISRNSSAILLRHYNWCVSRVHDEWFADEEKVRDAVGLLEKPVVDFPT 123

Query: 141 ELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSINDGPGCLMLRCPDPSCGAAV 200
           + EL CGICFE +  DK+ +AACGHPFC++CW GYI+T+INDGPGCL LRCPDPSC AAV
Sbjct: 124 DGELDCGICFETFLSDKLHAAACGHPFCDSCWEGYITTAINDGPGCLTLRCPDPSCRAAV 183

Query: 201 GQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDYAVDFIVGSGSYDVTCRCS 260
           GQDMINLLA D DK+KY  YFVRSYVEDNRKTKWCPAPGCDYAV+F+VGSG+YDV CRC 
Sbjct: 184 GQDMINLLAPDKDKQKYTSYFVRSYVEDNRKTKWCPAPGCDYAVNFVVGSGNYDVNCRCC 243

Query: 261 YSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI 320
           YSFCWNC EEAHRPVDC TV+KW+LKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI
Sbjct: 244 YSFCWNCAEEAHRPVDCDTVSKWVLKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI 303

Query: 321 TCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGVYDDAEKRREMAKNSLERY 380
           TCTPPCKFEFCWLCLG W++HGE+TGGFYACNRYE AKQ+G+YD+ EKRREMAKNSLERY
Sbjct: 304 TCTPPCKFEFCWLCLGAWTEHGEKTGGFYACNRYEAAKQDGIYDETEKRREMAKNSLERY 363

Query: 381 THYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQLKFISEAWLQIVECRRVL 440
           THYYERWATNQSSRQKAL DL +MQT  +EKLSDIQCQPESQLKFI EAWLQIVECRRVL
Sbjct: 364 THYYERWATNQSSRQKALLDLKKMQTDDIEKLSDIQCQPESQLKFIIEAWLQIVECRRVL 423

Query: 441 KWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKELQVYLNADGPSKDFNEFR 500
           KWTYAYG+Y+P++EH KR FFEYLQGEAESGLERLHQCAEKEL  YL+A GPS+DFNEFR
Sbjct: 424 KWTYAYGFYIPDQEHGKRVFFEYLQGEAESGLERLHQCAEKELLPYLDAKGPSEDFNEFR 483

Query: 501 TKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKSTGSCRSKGGKGKMSAFRA 560
           TKLAGLTSVT+NYFENLVRALENGL+DV+SH    RT+SSKS       GGK K S+ +A
Sbjct: 484 TKLAGLTSVTKNYFENLVRALENGLSDVNSHDAYDRTSSSKSL------GGKTKGSSSKA 543

Query: 561 SGSSRNIDDTDHWSCEHCTFANVKSATICQMCQQRR 595
           S S     D+ HW CE+CT+ N +S TICQMC+  R
Sbjct: 544 SSS-----DSSHWPCEYCTYVNPRSTTICQMCEHGR 567

BLAST of MC08g1970 vs. TAIR 10
Match: AT2G31510.1 (IBR domain-containing protein )

HSP 1 Score: 863.6 bits (2230), Expect = 9.2e-251
Identity = 420/568 (73.94%), Postives = 483/568 (85.04%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           M+SE+  DM DAH  D E  ED FYSGG DD    DSDD +  DY F++ D+DDS  + S
Sbjct: 1   MDSEE--DMLDAH--DMESGEDDFYSGGTDDCN--DSDDGE-PDYGFVEEDADDSAMIAS 60

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           +R Q+N+ +L E DIR+ Q ++I RVS VLSI++V ASILLR+++WSV +VHDEWFADEE
Sbjct: 61  HRSQKNFCVLREEDIRRHQMDNIERVSVVLSITEVEASILLRHFHWSVGRVHDEWFADEE 120

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           +VR+ VG+LE  V+  S++ EL CGICF+ Y  +KI S +CGHPFC TCWTGYIST+IND
Sbjct: 121 RVRKTVGILESHVVPPSDDSELTCGICFDSYPPEKIASVSCGHPFCTTCWTGYISTTIND 180

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLMLRCPDPSC AAVG DM++ LAS+D+K+KY RYF+RSY+EDNRK KWCPAPGCD+
Sbjct: 181 GPGCLMLRCPDPSCLAAVGHDMVDKLASEDEKEKYNRYFLRSYIEDNRKMKWCPAPGCDF 240

Query: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300
           A+DF+ GSG+YDV+C CS+SFCWNCTEEAHRPVDC TV+KWILKNSAESENMNWILANSK
Sbjct: 241 AIDFVAGSGNYDVSCLCSFSFCWNCTEEAHRPVDCSTVSKWILKNSAESENMNWILANSK 300

Query: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360
           PCP+CKRPIEKNQGCMH+TCTPPCK+EFCWLCLG W DHGERTGGFYACNRYE AKQEG 
Sbjct: 301 PCPRCKRPIEKNQGCMHMTCTPPCKYEFCWLCLGAWMDHGERTGGFYACNRYEVAKQEGQ 360

Query: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420
           YD+ E+RREMAKNSLERYTHYYERWA+NQ+SRQKA+ADL Q Q  +LEKLSD QC PESQ
Sbjct: 361 YDETERRREMAKNSLERYTHYYERWASNQTSRQKAMADLQQAQMQNLEKLSDKQCTPESQ 420

Query: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480
           LKFI EAWLQI+ECRRVLKWTYAYGYYLPE EHAKRQFFEYLQGEAESGLERLHQC EK+
Sbjct: 421 LKFILEAWLQIIECRRVLKWTYAYGYYLPEHEHAKRQFFEYLQGEAESGLERLHQCVEKD 480

Query: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTC-SRTASSK 540
           L  +L A+GPSKDFN+FRTKLAGLTSVT+NYFENLV+ALENGLADVDSH  C S++ SSK
Sbjct: 481 LVQFLIAEGPSKDFNDFRTKLAGLTSVTKNYFENLVKALENGLADVDSHAACSSKSTSSK 540

Query: 541 STGSCRSKGGKGKMSAFRASGSSRNIDD 568
           STG      GKGK S+ R  GSSRN DD
Sbjct: 541 STGCSSKTRGKGKGSS-RTGGSSRNPDD 560

BLAST of MC08g1970 vs. TAIR 10
Match: AT1G05890.1 (RING/U-box superfamily protein )

HSP 1 Score: 798.5 bits (2061), Expect = 3.6e-231
Identity = 386/566 (68.20%), Postives = 466/566 (82.33%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           M+S+D     D HD D  D +  +YSGG       D +D+D  D+ F + D+DD+  + S
Sbjct: 1   MDSDD-----DMHDMDSVDYD--YYSGG-----TYDDNDSDETDFGFGEADTDDAAIIAS 60

Query: 61  YRHQQNYIILAEADIRQRQEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEE 120
           YR + NY++L E DIR+ Q +D+ RVS VLSI+ V AS LL +Y+WSVSKV+DEWFADEE
Sbjct: 61  YRSKSNYVVLKEEDIRRHQNDDVGRVSAVLSITDVEASTLLLHYHWSVSKVNDEWFADEE 120

Query: 121 KVRRAVGLLEKPVLQSSNELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSIND 180
           +VRR VG+LE PV+ + +  E  CGICF+ Y+ ++I S +CGHPFC TCWTGYIST+IND
Sbjct: 121 RVRRTVGILEGPVVTTPDGREFTCGICFDSYTLEEIVSVSCGHPFCATCWTGYISTTIND 180

Query: 181 GPGCLMLRCPDPSCGAAVGQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDY 240
           GPGCLML+CPDPSC AA+G+DMI+ LAS +DK+KY+RYF+RSYVE NR+ KWCPAPGC++
Sbjct: 181 GPGCLMLKCPDPSCPAAIGRDMIDKLASKEDKEKYYRYFLRSYVEVNREMKWCPAPGCEH 240

Query: 241 AVDFIVGSGSYDVTCRCSYSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSK 300
           A+DF  G+ SYDV+C CS+SFCWNCTEEAHRPVDC TV KWILKNSAESENMNWILANSK
Sbjct: 241 AIDFAGGTESYDVSCLCSHSFCWNCTEEAHRPVDCDTVGKWILKNSAESENMNWILANSK 300

Query: 301 PCPKCKRPIEKNQGCMHITCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGV 360
           PCPKCKRPIEKN GCMH+TCTPPCKFEFCWLCL  W++HGERTGGFYACNRYE AKQEG+
Sbjct: 301 PCPKCKRPIEKNHGCMHMTCTPPCKFEFCWLCLNAWTEHGERTGGFYACNRYEAAKQEGL 360

Query: 361 YDDAEKRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQ 420
           YD+AE+RREMAKNSLERYTHYYERWA+NQ SRQKA+ DL +MQ+  L KLSDIQC PESQ
Sbjct: 361 YDEAERRREMAKNSLERYTHYYERWASNQVSRQKAMGDLQKMQSEKLGKLSDIQCTPESQ 420

Query: 421 LKFISEAWLQIVECRRVLKWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKE 480
           LKFI+EAWLQI+ECRRVLKWTYAYGYYL  ++HAK+ FFEYLQGEAESGLERLH+C EK+
Sbjct: 421 LKFIAEAWLQIIECRRVLKWTYAYGYYL--QDHAKKPFFEYLQGEAESGLERLHKCVEKD 480

Query: 481 LQVYLNADGPSKDFNEFRTKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKS 540
           ++V+  A+GPS++FN FRTKL GLTS+T+ +FENLV+ALENGLADVDS    S+ A+SK 
Sbjct: 481 IEVFELAEGPSEEFNHFRTKLTGLTSITKTFFENLVKALENGLADVDSQAASSKPANSKP 540

Query: 541 TGSCRSKGGKGKMSAFRASGSSRNID 567
           +   +  GGKGK S+ +  GSSRN D
Sbjct: 541 SSKTKG-GGKGKGSS-KNGGSSRNPD 550

BLAST of MC08g1970 vs. TAIR 10
Match: AT1G05890.2 (RING/U-box superfamily protein )

HSP 1 Score: 778.1 bits (2008), Expect = 5.1e-225
Identity = 386/608 (63.49%), Postives = 466/608 (76.64%), Query Frame = 0

Query: 1   MESEDELDMHDAHDDDDEDNEDAFYSGGDDDAAAIDSDDADVGDYEFIDNDSDDSDDMVS 60
           M+S+D     D HD D  D +  +YSGG       D +D+D  D+ F + D+DD+  + S
Sbjct: 1   MDSDD-----DMHDMDSVDYD--YYSGG-----TYDDNDSDETDFGFGEADTDDAAIIAS 60

Query: 61  YRHQ------------------------------------------QNYIILAEADIRQR 120
           YR +                                           NY++L E DIR+ 
Sbjct: 61  YRSKVASILVSCFGLLLQRWRMLEAVFCLFVCFFFFGLRLLLYSCNSNYVVLKEEDIRRH 120

Query: 121 QEEDIMRVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEEKVRRAVGLLEKPVLQSSN 180
           Q +D+ RVS VLSI+ V AS LL +Y+WSVSKV+DEWFADEE+VRR VG+LE PV+ + +
Sbjct: 121 QNDDVGRVSAVLSITDVEASTLLLHYHWSVSKVNDEWFADEERVRRTVGILEGPVVTTPD 180

Query: 181 ELELPCGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSINDGPGCLMLRCPDPSCGAAV 240
             E  CGICF+ Y+ ++I S +CGHPFC TCWTGYIST+INDGPGCLML+CPDPSC AA+
Sbjct: 181 GREFTCGICFDSYTLEEIVSVSCGHPFCATCWTGYISTTINDGPGCLMLKCPDPSCPAAI 240

Query: 241 GQDMINLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDYAVDFIVGSGSYDVTCRCS 300
           G+DMI+ LAS +DK+KY+RYF+RSYVE NR+ KWCPAPGC++A+DF  G+ SYDV+C CS
Sbjct: 241 GRDMIDKLASKEDKEKYYRYFLRSYVEVNREMKWCPAPGCEHAIDFAGGTESYDVSCLCS 300

Query: 301 YSFCWNCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHI 360
           +SFCWNCTEEAHRPVDC TV KWILKNSAESENMNWILANSKPCPKCKRPIEKN GCMH+
Sbjct: 301 HSFCWNCTEEAHRPVDCDTVGKWILKNSAESENMNWILANSKPCPKCKRPIEKNHGCMHM 360

Query: 361 TCTPPCKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGVYDDAEKRREMAKNSLERY 420
           TCTPPCKFEFCWLCL  W++HGERTGGFYACNRYE AKQEG+YD+AE+RREMAKNSLERY
Sbjct: 361 TCTPPCKFEFCWLCLNAWTEHGERTGGFYACNRYEAAKQEGLYDEAERRREMAKNSLERY 420

Query: 421 THYYERWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQLKFISEAWLQIVECRRVL 480
           THYYERWA+NQ SRQKA+ DL +MQ+  L KLSDIQC PESQLKFI+EAWLQI+ECRRVL
Sbjct: 421 THYYERWASNQVSRQKAMGDLQKMQSEKLGKLSDIQCTPESQLKFIAEAWLQIIECRRVL 480

Query: 481 KWTYAYGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKELQVYLNADGPSKDFNEFR 540
           KWTYAYGYYL  ++HAK+ FFEYLQGEAESGLERLH+C EK+++V+  A+GPS++FN FR
Sbjct: 481 KWTYAYGYYL--QDHAKKPFFEYLQGEAESGLERLHKCVEKDIEVFELAEGPSEEFNHFR 540

Query: 541 TKLAGLTSVTRNYFENLVRALENGLADVDSHGTCSRTASSKSTGSCRSKGGKGKMSAFRA 567
           TKL GLTS+T+ +FENLV+ALENGLADVDS    S+ A+SK +   +  GGKGK S+ + 
Sbjct: 541 TKLTGLTSITKTFFENLVKALENGLADVDSQAASSKPANSKPSSKTKG-GGKGKGSS-KN 592

BLAST of MC08g1970 vs. TAIR 10
Match: AT2G31760.1 (RING/U-box superfamily protein )

HSP 1 Score: 592.0 bits (1525), Expect = 5.2e-169
Identity = 283/504 (56.15%), Postives = 364/504 (72.22%), Query Frame = 0

Query: 35  IDSDDADVGDYEFIDNDSDDSDDMVSYRH----------QQNYIILAEADIRQRQEEDIM 94
           +D  D D+ D E  + ++ D     SY +          +++Y+I+ E +I + Q +DI 
Sbjct: 1   MDYSDDDMIDNESGEENNSDGGGNESYNYNAAVDTIILSEKSYVIIKEEEILKLQRDDIE 60

Query: 95  RVSTVLSISKVAASILLRYYNWSVSKVHDEWFADEEKVRRAVGLLEKPVLQ-SSNELELP 154
           RVST+L +S+V A +LL +Y+W VSK+ DEWF DEE++R+ VG+L++PV+  +  E+++ 
Sbjct: 61  RVSTILFLSQVEAIVLLLHYHWCVSKLEDEWFTDEERIRKTVGILKEPVVDVNGTEVDIQ 120

Query: 155 CGICFEIYSRDKIQSAACGHPFCNTCWTGYISTSINDGPGCLMLRCPDPSCGAAVGQDMI 214
           CGICFE Y+R +I S +CGHP+C TCWTGYI+T I DGPGCL ++CP+PSC A VGQDMI
Sbjct: 121 CGICFESYTRKEIASVSCGHPYCKTCWTGYITTKIEDGPGCLRVKCPEPSCYAVVGQDMI 180

Query: 215 NLLASDDDKKKYFRYFVRSYVEDNRKTKWCPAPGCDYAVDFIVGSGSYDVTCRCSYSFCW 274
           + +    DK KY+RYF+RSYVED +K KWCP+PGC+ AV+F   SG YDV C CSY FCW
Sbjct: 181 DEVTEKKDKDKYYRYFLRSYVEDGKKMKWCPSPGCECAVEFGESSG-YDVACLCSYRFCW 240

Query: 275 NCTEEAHRPVDCGTVAKWILKNSAESENMNWILANSKPCPKCKRPIEKNQGCMHITCTPP 334
           NC+E+AH PVDC TV+KWI KN  ESEN NWILANSKPCPKCKRPIEK+ GC H+TC+  
Sbjct: 241 NCSEDAHSPVDCETVSKWIFKNQDESENKNWILANSKPCPKCKRPIEKSHGCNHMTCSAS 300

Query: 335 CKFEFCWLCLGPWSDHGERTGGFYACNRYETAKQEGVYDDAEKRREMAKNSLERYTHYYE 394
           C   FCW+C   +SDH       YACN Y       V D    +R + ++ ++RYTHYY 
Sbjct: 301 CGHRFCWICGKSYSDH-------YACNNY-------VEDADHDKRTLLQSEIKRYTHYYV 360

Query: 395 RWATNQSSRQKALADLHQMQTVHLEKLSDIQCQPESQLKFISEAWLQIVECRRVLKWTYA 454
           RW  NQSSR KA++DL + Q+V L++LSD QC+P+  L+FI +AWLQI+ECRRVLKWTYA
Sbjct: 361 RWVENQSSRLKAMSDLEKFQSVQLKQLSDNQCKPKIDLQFIVDAWLQIIECRRVLKWTYA 420

Query: 455 YGYYLPEREHAKRQFFEYLQGEAESGLERLHQCAEKEL-QVYLNADGPSKDFNEFRTKLA 514
           YGYYL     AKR  FEYLQGEAE+GLERLH CAE EL Q ++ ++ PS  FN FR KL 
Sbjct: 421 YGYYLDNL--AKRPLFEYLQGEAETGLERLHHCAENELKQFFIKSEDPSDTFNAFRMKLT 480

Query: 515 GLTSVTRNYFENLVRALENGLADV 527
           GLT VT+ YF+NLV+ALENGLADV
Sbjct: 481 GLTKVTKTYFDNLVKALENGLADV 487