MC08g1921 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1921
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	MC08: 27402573 .. 27405416 (+)
RNA-Seq Expression	MC08g1921
Synteny	MC08g1921

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GACGAATCAAAACAAGAGCCAAAGGAAATGGCGAAGGGAGAATGGACCGATATAGACATAAAAATGGCTTGTATTTATTTCTTTGTGCACGTGCCTTGTATTTTTGCACCCTTTCACTTTAATTGGGGAGCGTTTTTTGTTGCTTTTGCATTATATTTTATCACGGGTCTCTTCGGTTTGAGTATTTCTTATCATAGAAACCTTTCGCACAAGAGTTTCAAACTTCCTAAATGGTTTGAATATTTGTTAGCTTACTGCGGACTTCATGCGCTCCAGGTTTGATATGTGATACTTTAGAGCCTGTTTCGTTATAATCCTCGTTATAAATTTAATGTTTTTGAATTTTGTGATAAAAAGTTTGAAAACAACATTTTATGTTTTCATTGTTTTCAAAATTTAAATTTGAAATTAGAGAAATAAAAATATTGATAGATATACTATTTCGTTTTATAAACTCATTTCTTTTTTCCTTTTTCCTTTTTTTAATTAAAACATGTATAATATAATATATAATATCGTAAATAATAATTATACAAAATTTATAACATATATGTTAATAAATTAAACTAATAATACTTTAGATTATGCTCAACATTTAGTTGGTAGCTACTGCTGATTTTCAAATGTTATTACTTAAATAAATTTTTAAAATGAGATTCAATTTCCAGATTTGCTATCAAACACATATATTAAAACACTAAAAACATGGAATACAACTATTTTTCAATGAATCCCTTGTTTTTTCATATTTCAATTTTCACATCACCCGTCAAACACGTTTTACCTTTTACTTTTATAATGAATAGAGAAATTTTGAAGTATTTATCTTTTGAATTTTTTCTTTTTTGGATTTTAGGGCGATCCAATTGATTGGGTGAGCACGCATAGATATCATCACCAATTTGTTGATACTGAAAGAGACCCGAATAGCCCCACCCAAGGATTGTTGTTTAGTTATATAACTTGGGCTTTTGATTCCTATGCTTTGACTAAAAAAGTCTGCCCGAAGTACTTTAGTGACTTTGAAGAAGAAGAAAGAGGCATCTTTGTAATGGCTAGAAAACATGGGAGACCAAATAATGTCGGTGACTTAGAGAAACAAGCTTTCTATCGATTTATTCACAAAACGTATCTTCTTCATGTAATTGCTCTTGCAGTCATTCTCTACGTCGTTGGAGGAATTCCTTTTCTTATATGGGGAACGGTAAAATAAAACCTAATATTCTGTTTTATTTGCTTTCAAGTACCAATCAAATGGATAATATATATATATATTTTACATTCTCTTTATTTGAACACCAAAGACTTTAAAAAAATTAATTTAAATTGAATTAAATAAGACTCCATCTCAAAATAAATTTATACTTCAGTTTTTTTTTTTTTTTGTTAGAAATTGGGTTGAATCATTAATAATCTCTTTACTAGTTTTTAAGCATATGAAACCTAAATAGGAGTAGTTTAATTAATTATAAGCAAAATATATAGAATATACAAACCATTTCTTTTTTTAGCTATAATAATATACCAACTAAATGAAGGACAAGTATATCACCTAAATTACAATTATACGTTACATTTTTTAAATTAATAATCTAGGATATAGTATAGTTAATAAAAATTAAAACAAAGATCAAAATATGATATATTGATCTACTCCTTTTCACGCAGTTTGTGAGGACTGTAATGCTTTTACATTCGACATTTATGGTGAGCTCAATATGCCATAAATGGGGCCATCAACAATGGGGTAATACTGGTTTGTCTAAAAATAATTGGTGAGTGTGTATTTTTTTAGAGTGAAAATAATTTAAATAGTTGATTGTAATTGTGATCTTTATTCCAATTATATTTATTAAATGGTAAGTTAATTGAATGATTTTTAGGTCAAATTTAAAACTGACAATTAATTTATTTATCATATATATATATATATATATTAAAATGTAGCCGTAGTTGCAAATATGACAAAAATATTTAGTAAGCTAAGATTCGCAAAATAGCCAACCATATTTAGTTTTTTAAATATAGCACTTTTGAAAAAAAAAATAGAGATCAAATTTTAAAATGACAACTTACCCAAATGATATATATCTATAATATATTTATAATATATCTGTAATATATATAACTACCGATTTATTTATGTGATATATATATGATATACTATTGACATGAGTGTCTGATAATATACTTGCAATATATATGTGATATACATCTGATGTACTACTGATATGAGTGTATGTGCAATATATATGTGATATACATTTGATATACTACTGATATGAGTGTCTGTACAATATGTATGTGATATACATTTGATATATTGTTGATGTGAGTTTGTGATATACTTAACTCATATAAGTGAAAATTTGTTTTATATTTGAAAATTTTAAAATACATGTAATATATTTTGAAATACATAAAAGATTTTTTATTTAAATGACTGATATGATTATTTTTTTTCAGTCACTTTATTTATATATATAGTGATAACTCAATGTAGCATTTATTCAATGTTGCATTGTAATATTATGTGATAATTTAATTCATAGAGCTATGAAATGTTGCATTTATAAAATAATCAAATACTTTTTAAAGAAAAATAAAAGAGTGTATTTTGCAGTTTACTGTAGTTCTTTTTGTATACTTCTTAGGTGGATGACTTTTATTTCATTTGGAGAAAATTGGCACAACAATCACCATGCCTTTGAGTATTCAGCTAGGATTGGGTTTGAATGGTGGCAGATTGACTTCGGTTGGTATATTATTTTGTTTCTCAAGACTATTGGAGTGGCCACTGACGTGAAGTTACCTCGTATGAGTTTGACTATAGAAGCTTTCAAC

mRNA sequence

GACGAATCAAAACAAGAGCCAAAGGAAATGGCGAAGGGAGAATGGACCGATATAGACATAAAAATGGCTTGTATTTATTTCTTTGTGCACGTGCCTTGTATTTTTGCACCCTTTCACTTTAATTGGGGAGCGTTTTTTGTTGCTTTTGCATTATATTTTATCACGGGTCTCTTCGGTTTGAGTATTTCTTATCATAGAAACCTTTCGCACAAGAGTTTCAAACTTCCTAAATGGTTTGAATATTTGTTAGCTTACTGCGGACTTCATGCGCTCCAGGGCGATCCAATTGATTGGGTGAGCACGCATAGATATCATCACCAATTTGTTGATACTGAAAGAGACCCGAATAGCCCCACCCAAGGATTGTTGTTTAGTTATATAACTTGGGCTTTTGATTCCTATGCTTTGACTAAAAAAGTCTGCCCGAAGTACTTTAGTGACTTTGAAGAAGAAGAAAGAGGCATCTTTGTAATGGCTAGAAAACATGGGAGACCAAATAATGTCGGTGACTTAGAGAAACAAGCTTTCTATCGATTTATTCACAAAACGTATCTTCTTCATGTAATTGCTCTTGCAGTCATTCTCTACGTCGTTGGAGGAATTCCTTTTCTTATATGGGGAACGTTTGTGAGGACTGTAATGCTTTTACATTCGACATTTATGGTGAGCTCAATATGCCATAAATGGGGCCATCAACAATGGGGTAATACTGGTTTGTCTAAAAATAATTGGTGGATGACTTTTATTTCATTTGGAGAAAATTGGCACAACAATCACCATGCCTTTGAGTATTCAGCTAGGATTGGGTTTGAATGGTGGCAGATTGACTTCGGTTGGTATATTATTTTGTTTCTCAAGACTATTGGAGTGGCCACTGACGTGAAGTTACCTCGTATGAGTTTGACTATAGAAGCTTTCAAC

Coding sequence (CDS)

GACGAATCAAAACAAGAGCCAAAGGAAATGGCGAAGGGAGAATGGACCGATATAGACATAAAAATGGCTTGTATTTATTTCTTTGTGCACGTGCCTTGTATTTTTGCACCCTTTCACTTTAATTGGGGAGCGTTTTTTGTTGCTTTTGCATTATATTTTATCACGGGTCTCTTCGGTTTGAGTATTTCTTATCATAGAAACCTTTCGCACAAGAGTTTCAAACTTCCTAAATGGTTTGAATATTTGTTAGCTTACTGCGGACTTCATGCGCTCCAGGGCGATCCAATTGATTGGGTGAGCACGCATAGATATCATCACCAATTTGTTGATACTGAAAGAGACCCGAATAGCCCCACCCAAGGATTGTTGTTTAGTTATATAACTTGGGCTTTTGATTCCTATGCTTTGACTAAAAAAGTCTGCCCGAAGTACTTTAGTGACTTTGAAGAAGAAGAAAGAGGCATCTTTGTAATGGCTAGAAAACATGGGAGACCAAATAATGTCGGTGACTTAGAGAAACAAGCTTTCTATCGATTTATTCACAAAACGTATCTTCTTCATGTAATTGCTCTTGCAGTCATTCTCTACGTCGTTGGAGGAATTCCTTTTCTTATATGGGGAACGTTTGTGAGGACTGTAATGCTTTTACATTCGACATTTATGGTGAGCTCAATATGCCATAAATGGGGCCATCAACAATGGGGTAATACTGGTTTGTCTAAAAATAATTGGTGGATGACTTTTATTTCATTTGGAGAAAATTGGCACAACAATCACCATGCCTTTGAGTATTCAGCTAGGATTGGGTTTGAATGGTGGCAGATTGACTTCGGTTGGTATATTATTTTGTTTCTCAAGACTATTGGAGTGGCCACTGACGTGAAGTTACCTCGTATGAGTTTGACTATAGAAGCTTTCAAC

Protein sequence

DESKQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN

Homology

[Close]

BLAST of MC08g1921 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-95
Identity = 159/294 (54.08%), Postives = 201/294 (68.37%), Query Frame = 0


Query: 4   KQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSIS 63
           K+E     + EW  +D     +   +H+  + APF FNW A  VAF LY +TGL G+++S
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 64  YHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLL 123
           +HRNLSHK+FKLPKW EYL AYCG  ALQG+PIDWVSTHRYHHQF D++RDP+SP  G  
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFW 206

Query: 124 FSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKT 183
           FS++ W FD+  +T++                       G PNNVGDLEKQ FYRF+  T
Sbjct: 207 FSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTT 266

Query: 184 YLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNN 243
           Y+LH +ALAV LY +GG PF++WG  VR V + H T++V+S CH WG Q W    LSKNN
Sbjct: 267 YILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNN 326

Query: 244 WWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
           WW+  ++FGE WHNNHHAFE+SAR G EWWQ+D  WY++ FL+ IG+ATDVKLP
Sbjct: 327 WWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

[Close]

BLAST of MC08g1921 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.8e-84
Identity = 145/299 (48.49%), Postives = 193/299 (64.55%), Query Frame = 0


Query: 2   ESKQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLS 61
           E  ++ + M + +W  +DI  A    FVH  C+ APF+F W A  VA  +Y + GL G++
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GIT 80

Query: 62  ISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQG 121
           +SYHRNL+H+SFK+PKW EY  AYCGL A+QGDPIDWVSTHRYHHQF D++RDP+SP +G
Sbjct: 81  VSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEG 140

Query: 122 LLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIH 181
             FS++ W FD+  L +K                       GR  NV DL++Q +Y+F+ 
Sbjct: 141 FWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQ 200

Query: 182 KTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSK 241
           +T L H++    +LY  GG+ FL WG  +   M  H T +++S+CH WG + W     S+
Sbjct: 201 RTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSR 260

Query: 242 NNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMS 301
           N WW++  SFGE+WHNNHHAFE SAR G EWWQID  WYI+ FL+ IG+ATDVKLP  S
Sbjct: 261 NVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSES 296

[Close]

BLAST of MC08g1921 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.8e-84
Identity = 144/283 (50.88%), Postives = 192/283 (67.84%), Query Frame = 0


Query: 15  WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFK 74
           W   DI    +   +HV C+ APF F+W AF+++  LY + G+FG ++SYHRNL+H+SFK
Sbjct: 37  WNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFK 96

Query: 75  LPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITWAFDSY 134
           LPK+ EY  AY GLHALQGDP+ WVSTHRYHH+F DT  DP+SP +G  FS+I W FDS 
Sbjct: 97  LPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDS- 156

Query: 135 ALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVI 194
                   KY             +  + GR  N GDL KQ++YRF+ +T++ HV   A +
Sbjct: 157 --------KY-------------ILEECGRYENAGDLLKQSYYRFLERTFVFHVYLQAAL 216

Query: 195 LYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGEN 254
           LY+ GG PF++WG  VRTV   H++++V+S+CH++GHQ W    LS NNW++  ++ GE 
Sbjct: 217 LYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEG 276

Query: 255 WHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
           WHNNHHAFEYSAR G EWWQID  WY+I  L+ +G+ATDVK+P
Sbjct: 277 WHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVP 297

[Close]

BLAST of MC08g1921 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.9e-83
Identity = 142/284 (50.00%), Postives = 192/284 (67.61%), Query Frame = 0


Query: 14  EWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSF 73
           +W   D+        +HV C+FAP  F+W +F+V FALY I G+FG ++S+HRNL+H+SF
Sbjct: 39  KWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRSF 98

Query: 74  KLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITWAFDS 133
           KLPK+ EY  AY GLHALQGDP+ WVSTHRYHH++ DT  DP+SP +G  F +I W FDS
Sbjct: 99  KLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFDS 158

Query: 134 YALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAV 193
                    KY             +  K GR  N GDL KQ++YRF+ +T++ HV   A 
Sbjct: 159 ---------KY-------------IIEKCGRYENAGDLMKQSYYRFLERTFVYHVYLQAA 218

Query: 194 ILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGE 253
           +LY+ GG PF++WG  VRT++  H +++V+S+CH+WG++ W    LS NNW++  ++ GE
Sbjct: 219 LLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGE 278

Query: 254 NWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
            WHNNHHAFEYSAR G EWWQID  WYII  L+ +G+ATD+K+P
Sbjct: 279 GWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVP 300

[Close]

BLAST of MC08g1921 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 305.1 bits (780), Expect = 9.2e-82
Identity = 142/283 (50.18%), Postives = 183/283 (64.66%), Query Frame = 0


Query: 15  WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFK 74
           W  +D       F VH   + APF+F W A +V F  Y I GL G+++SYHRNL+H+SFK
Sbjct: 36  WRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLAHRSFK 95

Query: 75  LPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITWAFDSY 134
           +PKW EYLLAYC L A+QGDPIDWVSTHRYHHQF D+ERDP+SP +G  FS++ W +DS 
Sbjct: 96  VPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSA 155

Query: 135 ALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVI 194
            L  K                       GR  NV DL++Q FYRF+ KT L H++ L   
Sbjct: 156 YLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHILGLGFF 215

Query: 195 LYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGEN 254
           L+ +GG+ F+ WG  V   + +H T +++S+CH WG + W     S+N WW++  SFGE+
Sbjct: 216 LFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGES 275

Query: 255 WHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
           WHNNHHAFE SAR G EWWQID  WYI+ F + IG+ATDVK+P
Sbjct: 276 WHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVP 295

[Close]

BLAST of MC08g1921 vs. NCBI nr
Match: XP_022144458.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 635 bits (1637), Expect = 7.93e-230
Identity = 297/298 (99.66%), Postives = 298/298 (100.00%), Query Frame = 0


Query: 10  MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 69
           MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS
Sbjct: 1   MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 60

Query: 70  HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITW 129
           HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP+SPTQGLLFSYITW
Sbjct: 61  HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPDSPTQGLLFSYITW 120

Query: 130 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 189
           AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI
Sbjct: 121 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 180

Query: 190 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 249
           ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI
Sbjct: 181 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 240

Query: 250 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN 307
           SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN
Sbjct: 241 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN 298

[Close]

BLAST of MC08g1921 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 462 bits (1188), Expect = 2.93e-161
Identity = 212/305 (69.51%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 3   SKQEPKEMAKG-------EWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFIT 62
           SKQEPKEM K        +WT +D  +AC  F VH+ CI APF FNW AF+VAF LY +T
Sbjct: 4   SKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVT 63

Query: 63  GLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP 122
           GLFG+++SYHRNL+HKSFKLPKW EYL AYCG+ ALQGDP+DWVSTHR HHQFVDTERDP
Sbjct: 64  GLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDP 123

Query: 123 NSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQA 182
           +SP +G  FS+I W FDSY LTKKVCPKYFS+F++ ER IFV+  KHG+P NV DLEKQA
Sbjct: 124 HSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQA 183

Query: 183 FYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWG 242
           FYRFIH+TYLLH I LAVILYVVGGIPFL+WG  VR V++LH T MV+S+CH WG Q W 
Sbjct: 184 FYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWT 243

Query: 243 NTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVK 300
              +SKNNWW++ I+FGE WHNNHHAFEYSA+ G EWWQ+DFGWY+ILFL+ IGVAT VK
Sbjct: 244 TGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVK 303

[Close]

BLAST of MC08g1921 vs. NCBI nr
Match: XP_038904662.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 457 bits (1176), Expect = 1.76e-159
Identity = 209/307 (68.08%), Postives = 243/307 (79.15%), Query Frame = 0


Query: 1   DESKQEPKEMAKG-------EWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYF 60
           D SKQE  E  K        EWT+ID  +AC+ F +H  CIFAPF F WGAF++A  LY 
Sbjct: 2   DVSKQELNERVKPTMSPRRREWTNIDKTIACLLFILHTLCIFAPFQFTWGAFWIALVLYV 61

Query: 61  ITGLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTER 120
           ITGLFG++ISYHRNLSH+SFKLPKW EYL AYCG HALQGDPIDWVSTHR HHQFVDT+ 
Sbjct: 62  ITGLFGITISYHRNLSHQSFKLPKWLEYLFAYCGAHALQGDPIDWVSTHRCHHQFVDTDN 121

Query: 121 DPNSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEK 180
           DP+SP QG  +S+ITW  DSY LTKKVC KYF+D +E ER +FV+  KH RP+NV DLEK
Sbjct: 122 DPHSPIQGFWYSHITWVLDSYNLTKKVCRKYFNDSKEAERNLFVLVMKHERPDNVKDLEK 181

Query: 181 QAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQ 240
           Q FY FIHKTY+LH IALAV+L +VGG+PF++WG  VR VML H  FMV+S+CHKWG QQ
Sbjct: 182 QIFYTFIHKTYILHPIALAVLLSMVGGVPFVLWGMCVRIVMLYHVIFMVNSVCHKWGKQQ 241

Query: 241 WGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATD 300
           W    LS+NNWW++F+SFGE WHNNHHAFEYSA+ G EWWQ+DFGWY+I+FLK IGVATD
Sbjct: 242 WNTNDLSRNNWWVSFLSFGEGWHNNHHAFEYSAKYGLEWWQLDFGWYVIMFLKVIGVATD 301

[Close]

BLAST of MC08g1921 vs. NCBI nr
Match: XP_022153632.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia] >XP_022153633.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 457 bits (1175), Expect = 6.32e-159
Identity = 206/305 (67.54%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 4   KQEPKEMA--------KGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFIT 63
           KQEPKEMA        + +WT +D  +AC  FFVH+  +FAPF+FNW AF++AFALY  T
Sbjct: 25  KQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVAT 84

Query: 64  GLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP 123
           GLFG+S+SYHRNL+HKSF LPKW EYL AYCG HALQGDPIDWV+THR HH+FVDTERDP
Sbjct: 85  GLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDP 144

Query: 124 NSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQA 183
           +SP QG  FS+ITW  DSY+LTK+VCPKYF+DF++ ER IFVM  KHGRP+NVGDLEKQ 
Sbjct: 145 HSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQT 204

Query: 184 FYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWG 243
           FYRFIHKTY LH I LA+ILY  GG PFLIWG  VR V++LH T M++S+CH WG Q W 
Sbjct: 205 FYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWR 264

Query: 244 NTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVK 300
              LS+NNWW++ +SFGE WHNNHHAFEYSAR G EWWQIDFGWY+++FL+ IG+AT+VK
Sbjct: 265 TGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVK 324

[Close]

BLAST of MC08g1921 vs. NCBI nr
Match: XP_038903746.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 455 bits (1171), Expect = 1.30e-158
Identity = 206/307 (67.10%), Postives = 243/307 (79.15%), Query Frame = 0


Query: 1   DESKQEPKEMAK-------GEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYF 60
           D SKQEP E+ K       G+W +ID   A + FF+H+PCIF PF F WGAF+VAF LY 
Sbjct: 2   DVSKQEPSELVKPLTPFERGKWNNIDKTTAFVIFFIHIPCIFGPFQFTWGAFWVAFVLYV 61

Query: 61  ITGLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTER 120
           ITGLFG++ISYHR+LSHKSFKLPKW EYL AYCG+HALQGDP+DWVSTHR HHQFVDTE+
Sbjct: 62  ITGLFGITISYHRHLSHKSFKLPKWLEYLFAYCGVHALQGDPMDWVSTHRCHHQFVDTEK 121

Query: 121 DPNSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEK 180
           DP+SP +G  FS+I W  DSY LTKKVCPKYF DF+  ER + ++  KHGRP NV DLEK
Sbjct: 122 DPHSPIEGFWFSHIAWTLDSYLLTKKVCPKYFRDFKGMERNMLMLNLKHGRPTNVVDLEK 181

Query: 181 QAFYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQ 240
           Q+FYRFI KTY LH IALAV+LY +GGIPF+IWG FVR  M +H  FMV+SI H+WG +Q
Sbjct: 182 QSFYRFIQKTYFLHPIALAVVLYAIGGIPFVIWGMFVRVTMFVHVMFMVNSIGHRWGTRQ 241

Query: 241 WGNTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATD 300
           W  + LS NNWW++ +SFG+ WHNNHHAFEYSAR G EWWQIDF WY+I+FL+ IGVAT+
Sbjct: 242 WNTSDLSTNNWWLSLLSFGDGWHNNHHAFEYSARHGLEWWQIDFSWYVIMFLQAIGVATE 301

[Close]

BLAST of MC08g1921 vs. ExPASy TrEMBL
Match: A0A6J1CTB0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014139 PE=3 SV=1)

HSP 1 Score: 635 bits (1637), Expect = 3.84e-230
Identity = 297/298 (99.66%), Postives = 298/298 (100.00%), Query Frame = 0


Query: 10  MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 69
           MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS
Sbjct: 1   MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLS 60

Query: 70  HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITW 129
           HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP+SPTQGLLFSYITW
Sbjct: 61  HKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPDSPTQGLLFSYITW 120

Query: 130 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 189
           AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI
Sbjct: 121 AFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVI 180

Query: 190 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 249
           ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI
Sbjct: 181 ALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFI 240

Query: 250 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN 307
           SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN
Sbjct: 241 SFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMSLTIEAFN 298

[Close]

BLAST of MC08g1921 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 462 bits (1188), Expect = 1.42e-161
Identity = 212/305 (69.51%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 3   SKQEPKEMAKG-------EWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFIT 62
           SKQEPKEM K        +WT +D  +AC  F VH+ CI APF FNW AF+VAF LY +T
Sbjct: 4   SKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLYIVT 63

Query: 63  GLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP 122
           GLFG+++SYHRNL+HKSFKLPKW EYL AYCG+ ALQGDP+DWVSTHR HHQFVDTERDP
Sbjct: 64  GLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTERDP 123

Query: 123 NSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQA 182
           +SP +G  FS+I W FDSY LTKKVCPKYFS+F++ ER IFV+  KHG+P NV DLEKQA
Sbjct: 124 HSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLEKQA 183

Query: 183 FYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWG 242
           FYRFIH+TYLLH I LAVILYVVGGIPFL+WG  VR V++LH T MV+S+CH WG Q W 
Sbjct: 184 FYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQSWT 243

Query: 243 NTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVK 300
              +SKNNWW++ I+FGE WHNNHHAFEYSA+ G EWWQ+DFGWY+ILFL+ IGVAT VK
Sbjct: 244 TGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVATHVK 303

[Close]

BLAST of MC08g1921 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 457 bits (1175), Expect = 3.06e-159
Identity = 206/305 (67.54%), Postives = 246/305 (80.66%), Query Frame = 0


Query: 4   KQEPKEMA--------KGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFIT 63
           KQEPKEMA        + +WT +D  +AC  FFVH+  +FAPF+FNW AF++AFALY  T
Sbjct: 25  KQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFALYVAT 84

Query: 64  GLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP 123
           GLFG+S+SYHRNL+HKSF LPKW EYL AYCG HALQGDPIDWV+THR HH+FVDTERDP
Sbjct: 85  GLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDTERDP 144

Query: 124 NSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQA 183
           +SP QG  FS+ITW  DSY+LTK+VCPKYF+DF++ ER IFVM  KHGRP+NVGDLEKQ 
Sbjct: 145 HSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQKVERNIFVMFSKHGRPDNVGDLEKQT 204

Query: 184 FYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWG 243
           FYRFIHKTY LH I LA+ILY  GG PFLIWG  VR V++LH T M++S+CH WG Q W 
Sbjct: 205 FYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWGKQPWR 264

Query: 244 NTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVK 300
              LS+NNWW++ +SFGE WHNNHHAFEYSAR G EWWQIDFGWY+++FL+ IG+AT+VK
Sbjct: 265 TGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGIATNVK 324

[Close]

BLAST of MC08g1921 vs. ExPASy TrEMBL
Match: A0A6J1E0E4 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111024778 PE=3 SV=1)

HSP 1 Score: 444 bits (1142), Expect = 1.37e-154
Identity = 206/301 (68.44%), Postives = 241/301 (80.07%), Query Frame = 0


Query: 3   SKQEPKEMAK---GEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFG 62
           SK+EP +M K    EW+ ID +MA  Y F+HV CIFAPFHFNW AF+VAFAL+ IT LFG
Sbjct: 4   SKEEPTKMVKLFKREWSMIDKEMAFFYVFIHVVCIFAPFHFNWSAFWVAFALHIITCLFG 63

Query: 63  LSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPT 122
           +SISYHRNLSH+SF+LP+W EYL AYCG+HALQGDPI WVSTHR HHQFVDTE+DP+SP 
Sbjct: 64  ISISYHRNLSHRSFQLPRWLEYLFAYCGVHALQGDPIGWVSTHRSHHQFVDTEKDPHSPI 123

Query: 123 QGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRF 182
           QG  FS++TW FDS +LTKKVCPKYF +FEE +R   V+  K+GRP+NV DLEKQ FYRF
Sbjct: 124 QGFWFSHLTWMFDSNSLTKKVCPKYFDNFEEVKRSGCVIVGKYGRPDNVSDLEKQTFYRF 183

Query: 183 IHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGL 242
           I KTYLLH IALA +LY +GG+PFLIWG  VRT+  LH TFMV+S+CH WG Q W N  L
Sbjct: 184 IQKTYLLHPIALAFLLYALGGLPFLIWGMCVRTITCLHITFMVNSVCHIWGKQPWKNGDL 243

Query: 243 SKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRM 300
           S+NN W+  + FGE WHNNHHAFEYSAR G EWWQIDFGWY+I  L+ IGVAT+VK+P  
Sbjct: 244 SRNNGWVGLLGFGEGWHNNHHAFEYSARFGLEWWQIDFGWYVIRLLQAIGVATNVKVPSQ 303

[Close]

BLAST of MC08g1921 vs. ExPASy TrEMBL
Match: A0A6J1GXQ0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457788 PE=3 SV=1)

HSP 1 Score: 444 bits (1142), Expect = 1.82e-154
Identity = 205/305 (67.21%), Postives = 238/305 (78.03%), Query Frame = 0


Query: 3   SKQEPKE-------MAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFIT 62
           SKQE K+           EWT  +  +A   F VH+ CIFAP  F W AF+VA ALY IT
Sbjct: 4   SKQESKDNMEPAMQFCSREWTIREKFVAFYIFIVHILCIFAPSQFTWNAFWVAIALYVIT 63

Query: 63  GLFGLSISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDP 122
           GLFG+SISYHRNLSH+SF LPKW EYL AYCG+HA QGDPIDWVSTHR HHQ+ DTE DP
Sbjct: 64  GLFGISISYHRNLSHRSFNLPKWLEYLFAYCGVHAFQGDPIDWVSTHRCHHQYADTEGDP 123

Query: 123 NSPTQGLLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQA 182
           +SPTQG  FSYITW FDS  LTKKVCP+YF D ++++R +F +  K+GRP+NVGDLEKQ 
Sbjct: 124 HSPTQGFWFSYITWLFDSITLTKKVCPEYFIDHKDKKRSVFTLVLKYGRPDNVGDLEKQT 183

Query: 183 FYRFIHKTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWG 242
           FYRFIH TY+LH+IALA++LYVVGG PFL+WG  VR VM LHSTFMV+SICH WG QQW 
Sbjct: 184 FYRFIHDTYVLHLIALAILLYVVGGTPFLVWGMGVRIVMSLHSTFMVNSICHSWGKQQWQ 243

Query: 243 NTGLSKNNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVK 300
              L++NNWW++ ISFGE WHNNHHAFEYSAR+G EWWQ+D GWY+I FL+ IGVATDVK
Sbjct: 244 TKDLTRNNWWLSLISFGEAWHNNHHAFEYSARVGIEWWQLDLGWYVIWFLQAIGVATDVK 303

[Close]

BLAST of MC08g1921 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 351.3 bits (900), Expect = 7.9e-97
Identity = 159/294 (54.08%), Postives = 201/294 (68.37%), Query Frame = 0


Query: 4   KQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSIS 63
           K+E     + EW  +D     +   +H+  + APF FNW A  VAF LY +TGL G+++S
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 64  YHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLL 123
           +HRNLSHK+FKLPKW EYL AYCG  ALQG+PIDWVSTHRYHHQF D++RDP+SP  G  
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFW 206

Query: 124 FSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKT 183
           FS++ W FD+  +T++                       G PNNVGDLEKQ FYRF+  T
Sbjct: 207 FSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTT 266

Query: 184 YLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNN 243
           Y+LH +ALAV LY +GG PF++WG  VR V + H T++V+S CH WG Q W    LSKNN
Sbjct: 267 YILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNN 326

Query: 244 WWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
           WW+  ++FGE WHNNHHAFE+SAR G EWWQ+D  WY++ FL+ IG+ATDVKLP
Sbjct: 327 WWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLP 358

[Close]

BLAST of MC08g1921 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 312.4 bits (799), Expect = 4.1e-85
Identity = 145/299 (48.49%), Postives = 193/299 (64.55%), Query Frame = 0


Query: 2   ESKQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLS 61
           E  ++ + M + +W  +DI  A    FVH  C+ APF+F W A  VA  +Y + GL G++
Sbjct: 21  EMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GIT 80

Query: 62  ISYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQG 121
           +SYHRNL+H+SFK+PKW EY  AYCGL A+QGDPIDWVSTHRYHHQF D++RDP+SP +G
Sbjct: 81  VSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEG 140

Query: 122 LLFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIH 181
             FS++ W FD+  L +K                       GR  NV DL++Q +Y+F+ 
Sbjct: 141 FWFSHLLWLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQ 200

Query: 182 KTYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSK 241
           +T L H++    +LY  GG+ FL WG  +   M  H T +++S+CH WG + W     S+
Sbjct: 201 RTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSR 260

Query: 242 NNWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLPRMS 301
           N WW++  SFGE+WHNNHHAFE SAR G EWWQID  WYI+ FL+ IG+ATDVKLP  S
Sbjct: 261 NVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSES 296

[Close]

BLAST of MC08g1921 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 305.1 bits (780), Expect = 6.5e-83
Identity = 142/283 (50.18%), Postives = 183/283 (64.66%), Query Frame = 0


Query: 15  WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFK 74
           W  +D       F VH   + APF+F W A +V F  Y I GL G+++SYHRNL+H+SFK
Sbjct: 36  WRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLAHRSFK 95

Query: 75  LPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITWAFDSY 134
           +PKW EYLLAYC L A+QGDPIDWVSTHRYHHQF D+ERDP+SP +G  FS++ W +DS 
Sbjct: 96  VPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSA 155

Query: 135 ALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVI 194
            L  K                       GR  NV DL++Q FYRF+ KT L H++ L   
Sbjct: 156 YLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHILGLGFF 215

Query: 195 LYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGEN 254
           L+ +GG+ F+ WG  V   + +H T +++S+CH WG + W     S+N WW++  SFGE+
Sbjct: 216 LFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGES 275

Query: 255 WHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
           WHNNHHAFE SAR G EWWQID  WYI+ F + IG+ATDVK+P
Sbjct: 276 WHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVP 295

[Close]

BLAST of MC08g1921 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 295.8 bits (756), Expect = 4.0e-80
Identity = 136/283 (48.06%), Postives = 185/283 (65.37%), Query Frame = 0


Query: 15  WTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSISYHRNLSHKSFK 74
           WT  D+    I+   H+  + APF+F+W AF+V   L FI G+  +++SYHRNLSH+SF 
Sbjct: 91  WTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLSYHRNLSHRSFD 150

Query: 75  LPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGLLFSYITWAFDSY 134
           LPKW EYL AY G+ A QGDPI+WVS HRYHH+  +T+RDP+SPTQG  FS++ W FD+ 
Sbjct: 151 LPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWIFDTS 210

Query: 135 ALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHKTYLLHVIALAVI 194
           ++ +                        G   NV DL +Q FYRF+ +T LLH++A + +
Sbjct: 211 SILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHMMAYSFL 270

Query: 195 LYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKNNWWMTFISFGEN 254
            Y  GG+P L+WG  +   + LH TF+V+S+CH WG + W  +  SKNNWW+  ++ GE 
Sbjct: 271 FYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEG 330

Query: 255 WHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
           WHNNHHAFE+SAR G EWWQ+D  W +I FL+ IG+AT+VKLP
Sbjct: 331 WHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLP 350

[Close]

BLAST of MC08g1921 vs. TAIR 10
Match: AT1G06350.1 (Fatty acid desaturase family protein )

HSP 1 Score: 278.5 bits (711), Expect = 6.5e-75
Identity = 133/295 (45.08%), Postives = 184/295 (62.37%), Query Frame = 0


Query: 3   SKQEPKEMAKGEWTDIDIKMACIYFFVHVPCIFAPFHFNWGAFFVAFALYFITGLFGLSI 62
           S  + +   + +W  +D+  A +   VH  C+ APF+F W A      L+ +T L  ++ 
Sbjct: 17  STMQKRAYFQRQWPLVDVVRASVVVIVHFLCLLAPFNFKWEALRFGLVLFALTTL-SITF 76

Query: 63  SYHRNLSHKSFKLPKWFEYLLAYCGLHALQGDPIDWVSTHRYHHQFVDTERDPNSPTQGL 122
           S+HRNLSH+SFK+PKW EY  AY  + ALQGDP+DWVS HR+HHQF D++RDP+SP +GL
Sbjct: 77  SFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDWVSIHRFHHQFTDSDRDPHSPKEGL 136

Query: 123 LFSYITWAFDSYALTKKVCPKYFSDFEEEERGIFVMARKHGRPNNVGDLEKQAFYRFIHK 182
           LFS+I W FD+  +      KY                K G  +NV DL+KQ FY+F+ +
Sbjct: 137 LFSHILWIFDTQYI------KY----------------KCGGRDNVLDLKKQWFYKFLRR 196

Query: 183 TYLLHVIALAVILYVVGGIPFLIWGTFVRTVMLLHSTFMVSSICHKWGHQQWGNTGLSKN 242
           T  +H++    ILY+ GG+P+L  G  V   +  H T++V+S CH WG + W     S+N
Sbjct: 197 TIAVHILMFWTILYLYGGLPYLTCGGGVGIFIGYHVTWLVNSACHIWGSRSWNTKDTSRN 256

Query: 243 NWWMTFISFGENWHNNHHAFEYSARIGFEWWQIDFGWYIILFLKTIGVATDVKLP 298
            WW++  + GE+WHNNHHAFE SAR G EWWQID  WY+I   + +G+ATDVKLP
Sbjct: 257 VWWLSLFTMGESWHNNHHAFESSARQGLEWWQIDITWYLIRLFEVLGIATDVKLP 288

The following BLAST results are available for this feature:

BLAST of MC08g1921 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	1.1e-95	54.08	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
O65797	5.8e-84	48.49	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
P0DOW3	9.8e-84	50.88	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]
P0DOW2	2.9e-83	50.00	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9SID2	9.2e-82	50.18	Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=...	[more]

BLAST of MC08g1921 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144458.1	7.93e-230	99.66	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022153644.1	2.93e-161	69.51	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_038904662.1	1.76e-159	68.08	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]
XP_022153632.1	6.32e-159	67.54	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_038903746.1	1.30e-158	67.10	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]

BLAST of MC08g1921 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTB0	3.84e-230	99.66	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DJP0	1.42e-161	69.51	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DHZ7	3.06e-159	67.54	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1E0E4	1.37e-154	68.44	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GXQ0	1.82e-154	67.21	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of MC08g1921 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	7.9e-97	54.08	fatty acid desaturase 5	[more]
AT1G06080.1	4.1e-85	48.49	delta 9 desaturase 1	[more]
AT2G31360.1	6.5e-83	50.18	16:0delta9 desaturase 2	[more]
AT3G15870.1	4.0e-80	48.06	Fatty acid desaturase family protein	[more]
AT1G06350.1	6.5e-75	45.08	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 102..131 score: 39.82 coord: 65..85 score: 33.08
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 11..298
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 41..295 e-value: 2.02465E-61 score: 191.23
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 47..277 e-value: 9.7E-18 score: 64.9
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 11..298

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g1921.1	MC08g1921.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water